LOCUS       NC_015166               6277 bp    DNA     circular CON 06-JUL-2013
DEFINITION  Bacteroides salanitronis DSM 18170 plasmid pBACSA03, complete
            sequence.
ACCESSION   NC_015166
VERSION     NC_015166.1  GI:324959701
DBLINK      Project: 63269
            BioProject: PRJNA63269
KEYWORDS    .
SOURCE      Bacteroides salanitronis DSM 18170
  ORGANISM  Bacteroides salanitronis DSM 18170
            Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales;
            Bacteroidaceae; Bacteroides.
REFERENCE   1  (bases 1 to 6277)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Goodwin,L., Pitluck,S.,
            Kyrpides,N., Mavromatis,K., Ivanova,N., Mikhailova,N., Teshima,H.,
            Misra,M., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L.,
            Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D.,
            Gronow,S., Wellnitz,S., Brambilla,E., Klenk,H.-P. and Eisen,J.A.
  CONSRTM   US DOE Joint Genome Institute (JGI-PGF)
  TITLE     The complete sequence of plasmid3 of Bacteroides salanitronis DSM
            18170
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6277)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2011) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 6277)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Goodwin,L., Pitluck,S.,
            Kyrpides,N., Mavromatis,K., Ivanova,N., Mikhailova,N., Teshima,H.,
            Misra,M., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L.,
            Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D.,
            Gronow,S., Wellnitz,S., Brambilla,E., Klenk,H.-P. and Eisen,J.A.
  CONSRTM   US DOE Joint Genome Institute (JGI-PGF)
  TITLE     Direct Submission
  JOURNAL   Submitted (17-FEB-2011) US DOE Joint Genome Institute, 2800
            Mitchell Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence is identical to CP002533.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 4087335
            Source DNA and organism available from Hans-Peter Klenk at the
            German Collection of Microorganisms and Cell Cultures (DSMZ)
            (hans-peter.klenk@dsmz.de)
            Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu)
                      David Bruce (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Finishing done by JGI-LANL
            Annotation by JGI-ORNL and JGI-PGF
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            
            ##MIGS-Data-START##
            investigation_type  :: bacteria_archaea
            project_name        :: Bacteroides salanitronis BL78, DSM 18170
            collection_date     :: isolated by  P. T. N. Lan
            lat_lon             :: Missing
            depth               :: Missing
            alt_elev            :: Missing
            country             :: Japan
            environment         :: Host
            num_replicons       :: 4
            ref_biomaterial     :: DSM 18170, CCUG 54637, JCM 13657
            biotic_relationship :: Free living
            trophic_level       :: Missing
            rel_to_oxygen       :: Anaerobe
            isol_growth_condt   :: 17158987
            sequencing_meth     :: WGS
            assembly            :: Newbler v. 2.3 (pre-release)
            finishing_strategy  :: Finished
            GOLD Stamp ID       :: Gi04003
            Type Strain         :: Yes
            Funding Program     :: DOE-GEBA 2009
            Gene Calling Method :: Prodigal 1.4, GenePRIMP
            Isolation Site      :: caecum of chicken
            Host Name           :: caecum of healthy chicken
            Cell Shape          :: Rod-shaped
            Motility            :: Nonmotile
            Sporulation         :: Nonsporulating
            Temperature Range   :: Mesophile
            Temperature Optimum :: 37CC
            Gram Staining       :: Gram-
            Diseases            :: None
            ##MIGS-Data-END##
            
            ##Genome-Assembly-Data-START##
            Finishing Goal           :: Finished
            Current Finishing Status :: Finished
            Assembly Method          :: Newbler v. 2.3
            Genome Coverage          :: 30x
            Sequencing Technology    :: 454/Illumina
            ##Genome-Assembly-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6277
                     /organism="Bacteroides salanitronis DSM 18170"
                     /mol_type="genomic DNA"
                     /strain="DSM 18170"
                     /isolation_source="cecum"
                     /host="chicken"
                     /culture_collection="DSMZ:DSM 18170"
                     /db_xref="taxon:667015"
                     /plasmid="pBACSA03"
                     /country="Japan"
                     /collected_by="P.T.N. Lan"
     gene            complement(108..1007)
                     /locus_tag="Bacsa_3730"
                     /db_xref="GeneID:10228987"
     CDS             complement(108..1007)
                     /locus_tag="Bacsa_3730"
                     /note="KEGG: bfr:BF0643 clindamycin resistance transfer
                     factor BtgB;
                     SPTR: MobB"
                     /codon_start=1
                     /transl_table=11
                     /product="clindamycin resistance transfer factor BtgB"
                     /protein_id="YP_004260699.1"
                     /db_xref="GI:324959702"
                     /db_xref="GeneID:10228987"
                     /translation="MNIKIQGGGSGTYANTGSCTEVTTYLQHEDLERMKNGREVQPFF
                     NNSRDYISAQEVTFKIDNNKAKLSRNDAKFYVITVSPSSRELEKMGKTEKEQAEAMRR
                     YVRDDVMQHYAEGFGKGLNKEDIEYYGKIHFERKGADRYDMHAHIIVSRKDRSNTRKL
                     SPKTNHTGKKNCGNVKGGFDRTDFFRKCETSFDKRTGYDRAPEQTFDYLNTMKNGTPK
                     EIFQKKEWAERVNHERLEKMKAEWSRDLQEQHQEQGREEIQQQGNNISQVPEINQASQ
                     RKKQQEEELNQPRKRSRGFGMGM"
     gene            complement(1004..1606)
                     /locus_tag="Bacsa_3731"
                     /db_xref="GeneID:10228980"
     CDS             complement(1004..1606)
                     /locus_tag="Bacsa_3731"
                     /note="KEGG: bfr:BF0644 clindamycin resistance transfer
                     factor BtgA;
                     SPTR: MobA"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_004260700.1"
                     /db_xref="GI:324959703"
                     /db_xref="GeneID:10228980"
                     /translation="MEKDSKTTVAVERTTFAKLDRLAKANSVSKMEYITHAINYFEKY
                     GINPVEHESPAQEMQRLIKRMDQVFAFLKKQETDLVRPACEALAGASTQITISLSSLL
                     SEEKFRRFLKDNKELLAGIINSSDNNGRAIGRAEKAVRDADSTIRKNQDIIFKRIEAS
                     ETAQLKAMQHIVSFLDAKGKTGLLDDINKVYKKEKERRGL"
     gene            complement(2097..2318)
                     /locus_tag="Bacsa_3732"
                     /db_xref="GeneID:10228981"
     CDS             complement(2097..2318)
                     /locus_tag="Bacsa_3732"
                     /note="InterPro IPR009614;
                     KEGG: cli:Clim_0726 addiction module toxin, Txe/YoeB
                     family;
                     PFAM: Addiction module toxin, Txe/YoeB;
                     SPTR: Predicted protein;
                     TIGRFAM: Addiction module toxin, Txe/YoeB;
                     PFAM: Plasmid encoded toxin Txe;
                     TIGRFAM: toxin-antitoxin system, toxin component, Txe/YoeB
                     family"
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, Txe/YoeB family"
                     /protein_id="YP_004260701.1"
                     /db_xref="GI:324959704"
                     /db_xref="GeneID:10228981"
                     /translation="MRKSSPQLFRKLEKLLDELREHPYTGTGHPEQLRYLEGVWSRQL
                     DKKNRIRYMVNETTVIVFIISAWGHYDDK"
     misc_feature    complement(2109..>2252)
                     /locus_tag="Bacsa_3732"
                     /note="Plasmid encoded toxin Txe; Region: Plasmid_Txe;
                     cl17389"
                     /db_xref="CDD:247943"
     gene            complement(2359..2625)
                     /locus_tag="Bacsa_3733"
                     /db_xref="GeneID:10228982"
     CDS             complement(2359..2625)
                     /locus_tag="Bacsa_3733"
                     /note="KEGG: slp:Slip_0182 prevent-host-death family
                     protein;
                     SPTR: Predicted protein"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_004260702.1"
                     /db_xref="GI:324959705"
                     /db_xref="GeneID:10228982"
                     /translation="MQIVTTREFRANQKKYFEMAETETILVSRRNAAPIMVCAVREGD
                     FPSKEELAAIQRGIEDIRNGNTFRMAKNESLDDFLNRIEGEGNV"
     gene            3078..4058
                     /locus_tag="Bacsa_3734"
                     /db_xref="GeneID:10228983"
     CDS             3078..4058
                     /locus_tag="Bacsa_3734"
                     /note="COGs: COG5527 Protein involved in initiation of
                     plasmid replication;
                     InterPro IPR000525;
                     KEGG: aps:CFPG_P1-1 replication protein A;
                     PFAM: Initiator Rep protein;
                     SPTR: RepA protein;
                     PFAM: Initiator Replication protein"
                     /codon_start=1
                     /transl_table=11
                     /product="initiator RepB protein"
                     /protein_id="YP_004260703.1"
                     /db_xref="GI:324959706"
                     /db_xref="GeneID:10228983"
                     /translation="MAIVNKDLIQSYIMTTAKYDFNVYEKRIIYRLVEMAQGELQGIK
                     FADNCQKIEHDLFSCVNITMPIACLLNGEDDKNYARVKEALQSLQRKIFEYEDDEIWQ
                     SISIIALPYIRKRSSVLSFQVHPRVWDCILDFSKGFRKYELKAAMSFKSQFSMRFYEL
                     LSNQKTPLIYSIEQLKEMFCVTDKYERINDFIRYVVDAAKKELDEISPYTFEYTPLKT
                     GRKITAIKFYPVYQPEHRDSDLEKHDLQKQTALSWSLAPEIRSYLKNSIEFSDKEIKN
                     NLDLFISAQTLLPDFLNELAILKGKSREKKNPKGYIINAIKGKIKDRKKL"
     misc_feature    3102..3761
                     /locus_tag="Bacsa_3734"
                     /note="Initiator Replication protein; Region: Rep_3;
                     pfam01051"
                     /db_xref="CDD:216266"
     gene            4088..4288
                     /locus_tag="Bacsa_3735"
                     /db_xref="GeneID:10228984"
     CDS             4088..4288
                     /locus_tag="Bacsa_3735"
                     /note="KEGG: hvo:HVO_A0463 ribonuclease H I;
                     SPTR: Predicted protein"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_004260704.1"
                     /db_xref="GI:324959707"
                     /db_xref="GeneID:10228984"
                     /translation="MKEGDLILVSAEATGLGKPMEAIIDKIETFMGQTLVTVTYTQPD
                     ALSGFGGCFVDSHITLSEKKAK"
     gene            4402..4944
                     /locus_tag="Bacsa_3736"
                     /db_xref="GeneID:10228985"
     CDS             4402..4944
                     /locus_tag="Bacsa_3736"
                     /note="KEGG: hmg:100200897 similar to predicted protein;
                     SPTR: Predicted protein"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_004260705.1"
                     /db_xref="GI:324959708"
                     /db_xref="GeneID:10228985"
                     /translation="MTDKATIQPKSKPEKTTISISGETSERLEIYCRANGILRKEFVS
                     LALEYFERTGFDLKSNALDYSPLEKIIGELNEVKATMQTSNEGTEAIRQLLQAVREQT
                     AKQLPAPELIAQATEAKAKAESLATERAKDLQLLQEENNRLRNEIKVLQEYKEKAHRE
                     LCRVRDEQKTFGKIKVNTEL"
     gene            complement(5220..6206)
                     /locus_tag="Bacsa_3737"
                     /db_xref="GeneID:10228986"
     CDS             complement(5220..6206)
                     /locus_tag="Bacsa_3737"
                     /note="KEGG: dal:Dalk_2441 hypothetical protein;
                     SPTR: Putative uncharacterized protein"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_004260706.1"
                     /db_xref="GI:324959709"
                     /db_xref="GeneID:10228986"
                     /translation="MKKLFYSLMLCSFFVACSNEDDIVMQDDQSFSNEEVLTRSASMR
                     RSLTPQEADMLKKAFPNLKTDNVTVTGEATYTYNCIAYSMGITSKWINPEELYNDFIN
                     QYKNAKSLYGASSNYEQTSTQGSGADVDGWGLNTYKMTHGSVIYSGNTWESKLGPNIR
                     ITHGRTELNGSEYGSILVSFVKQNSKISDLKEVAEEVSKEDIVLSDLEKNKVLERSKK
                     VDSEIVSKFESLIAAWKNEINTNPKTMFSSSTLDYKQLPQFKSLQEMGSVIIPLIMEK
                     LLDEENFFMLPLYDTLQLDSNLKIVYNKNDRKCLEGEQNRARRTVRMWVNSL"
     sig_peptide     complement(6153..6206)
                     /locus_tag="Bacsa_3737"
                     /note="Signal predicted by SignalP 3.0 HMM"
ORIGIN      
        1 ttaactattc cggtgggctt ttttcttctt caaggtagat ttcctgaaga agttcatctt
       61 tactcgttac ctcataacga cacaaaaact atactctcag aataaaatca catacccatt
      121 ccaaaacccc ggcttctttt cctgggctgg ttgagttctt cctcctgttg ttttttcctt
      181 tgcgatgcct ggttgatttc cggaacctgg gaaatattgt ttccctgttg ttggatttct
      241 tcccgacctt gttcctggtg ctgttcctga agatcccggc tccattcggc tttcatcttc
      301 tcaagccgtt catggttcac acgttccgcc cactcctttt tctggaagat ttctttcgga
      361 gttccgttct tcatggtatt gagatagtcg aaggtctgtt ccggtgcccg gtcataccct
      421 gtgcgcttgt caaatgaggt ttcacacttt cggaaaaaat ctgttctgtc aaaaccgcct
      481 ttcacgtttc cgcagttctt cttcccggtg tggttggtct ttgggcttag cttcctggtg
      541 ttgctcctgt ccttccggct gacgatgata tgtgcgtgca tgtcgtaccg gtcggctccc
      601 ttcctctcga aatggatctt tccgtaatac tcgatatcct ctttgttcag gccttttccg
      661 aacccttccg catagtgctg catcacatca tcacggacat accttctcat ggcttcagcc
      721 tgttccttct ctgtcttgcc cattttctcc agctcccggc tggaagggct taccgtgatc
      781 acatagaact tcgcatcgtt ccggcttaat tttgccttgt tgttgtctat cttgaaggtt
      841 acttcctgtg cgcttatgta gtcacgtgag ttattgaaaa acggctgcac ttcccggccg
      901 ttcttcatgc gctccagatc ctcatgctgg agataggtcg ttacttctgt gcagctccct
      961 gtattggcat aagttccgga accgccaccc tgaattttta tattcatagc cccctccttt
     1021 ccttttcctt cttatagacc ttgtttatgt catcgagcag cccggtcttt ccttttgcat
     1081 cgaggaagga aacgatatgt tgcatggcct tgagttgtgc cgtttcgctg gcttctatcc
     1141 gcttgaagat aatatcctgg ttcttcctga tagttgaatc tgcatcccgc accgcttttt
     1201 ctgccctgcc tatggccctg ccgttgttgt cggaactgtt gattatcccg gcaagcagtt
     1261 ccttgttgtc cttcaggaac cgcctgaact tttcttccga aagtaaggat gaaaggctta
     1321 tggttatctg tgtgctggct ccggccagag cttcacaagc cggacgcaca agatccgttt
     1381 cctgtttctt caggaatgcg aacacctggt ccatgcgctt gataagtctc tgcatttcct
     1441 gtgccggact ttcatgctcg accgggttta taccgtactt ctcaaagtag tttatggcat
     1501 gagtaatata ctccattttt gacacactgt ttgccttggc cagacggtca agtttagcaa
     1561 aagtagtacg ttctaccgct accgttgttt tactgtcttt ttccatataa ttataaggct
     1621 tattataacg aattaatata ctaactagtt ggtttacaag atagtatatt tattcattat
     1681 aaccaaatta tattggtata attttctaaa tagctgattt tcagctaaag accccgtatg
     1741 gcaagaggaa aaaacagggc ttgcccggtt tcctcttgct gttctatcga acaccctccc
     1801 cagcgcgaaa ttagcaaagg ggacggggag agatagaacc tatcccacac acgtaaacac
     1861 ccacgaaaac cgcacgcccg gtacacaaca gaaataccca acaaaaccgc acaacagaac
     1921 gtgtccggaa caccacaaaa aactcatcgg tcggggagcg ccgcacggca agggggtgta
     1981 ccaacactcc cgacgagtgc agccgtttgc ttggatatat gatataagtt attttgattc
     2041 tgtcacaaat tcaaaaggat tgcagtatgt gccgtttcgg aacttactgc aatccttcat
     2101 ttgtcgtcat aatgccccca ggctgaaatg ataaacacaa ttacggtagt ctcgttgacc
     2161 atatacctga tacggttctt cttgtccagc tgccttgacc agacaccctc caggtatctc
     2221 aactgttccg gatgccctgt acccgtgtac gggtgttccc ggagttcatc aagcagcttc
     2281 tccagcttcc tgaaaagctg cggagaggat ttcctcatca acaggacatg ttttaccgct
     2341 tctttggaaa attctatctc atacattgcc ttcgccctct atacggttaa gaaaatcatc
     2401 cagagactcg tttttagcca ttctgaaggt atttccgttc cggatatctt caatacctct
     2461 ctggatggcc gcaagttctt cctttgaagg gaaatcacct tctctgactg cacacaccat
     2521 aataggggct gcattgcgtc tggagaccag gatcgtttcc gtctctgcca tttcgaagta
     2581 tttcttctga ttggcacgaa attcgcgtgt tgtaacaatc tgcatagttc taatatattg
     2641 gttttcaccg caaaaatagg gataaaaatc aataaaacaa aagttttgtg tacttatttg
     2701 tgtacacgcg cggaaaattc gttcctcaaa gaacgctgcg ctggcttttg ctttgctttc
     2761 acaaacattc aaaaactcgt ttttcacgga tttggaggtc tggaagccac attcaggcta
     2821 aaattttatg acttgttttt aagcattact tgttatatgc ttatcaactt ttaaaaaatg
     2881 cactttaaca atctgacaaa cagaatatta caatcatcag cagcgatcta actacgagtt
     2941 tttgaatact aactgcgagt ttttgaatac tatttcaaag tttaactacg agtttttgaa
     3001 tactaatcac acaatctacg agttttattt gtaaaactcg tttttaatac ttatatttgc
     3061 gcaaaacact gataattatg gcaatagtaa acaaagacct tatccaaagt tatattatga
     3121 ctactgcaaa atatgacttt aatgtgtacg aaaagcgtat catttatcgt ctcgtagaaa
     3181 tggctcaagg agagctgcaa ggtataaagt tcgctgataa ctgtcagaag atagagcatg
     3241 atcttttcag ttgcgtaaat ataaccatgc ctatcgcctg cctgcttaat ggagaggacg
     3301 acaagaatta tgcgagagta aaagaagccc tacagtcgct tcagcgaaag atctttgagt
     3361 atgaagatga tgagatctgg caaagtatct ctatcatagc cttgccttat atacggaagc
     3421 gttcttcagt attatccttt caggtacacc cacgtgtatg ggactgcata cttgatttct
     3481 caaagggttt taggaaatac gaactgaaag cagccatgag ctttaagagc cagttttcaa
     3541 tgcgctttta tgagttgttg agcaaccaga aaactcctct gatatactct atagaacagc
     3601 taaaggaaat gttttgcgta acagacaaat acgagcgcat caacgatttt atacgttatg
     3661 ttgtggatgc tgcaaaaaaa gagcttgatg aaatcagccc atataccttt gagtacacac
     3721 cgctcaaaac agggcgaaag ataacggcca taaagtttta tccggtatat caaccggaac
     3781 accgtgacag tgatttggaa aaacatgacc ttcaaaaaca gactgcttta agctggtcgt
     3841 tagctccgga aataagaagt tatcttaaaa actcgattga gttttcagat aaagagatta
     3901 aaaacaatct agatctgttt atatcagcac aaaccttact tccagacttc cttaatgaac
     3961 tcgccattct aaaagggaaa tcaagggaaa agaagaaccc aaaaggttat ataatcaatg
     4021 ctatcaaggg aaaaattaaa gacagaaaga aactttgatt ttgtcacaca atcaaaaaaa
     4081 caaagacatg aaagaaggag atttgatatt ggtatcagct gaagctacag gcttaggaaa
     4141 gccaatggaa gccattatag acaagattga gacattcatg ggacaaaccc ttgtaacagt
     4201 cacttacacg caaccagacg ccttatccgg cttcggaggg tgctttgtgg attctcatat
     4261 cactttaagc gaaaaaaagg ccaagtaacc tgaatatatt tcaggaatat tcggtatatt
     4321 tacagcatat tcaattaaaa agaagcataa aaccagcata tcaagcatat tataagcata
     4381 tttatatcaa tcggttaagg tatgacagat aaagcaacca tacaacccaa aagcaagccg
     4441 gaaaagacta caatctcaat aagcggagaa acatctgaac ggctggagat atactgccgg
     4501 gcaaacggca tattacggaa agagttcgtt tctctcgccc tggaatactt tgaacgcaca
     4561 ggtttcgacc tgaagagtaa cgcacttgat tactccccct tggaaaagat catcggagaa
     4621 ttgaatgaag taaaagcgac catgcagaca agcaatgagg gaacagaagc gattcgccaa
     4681 ctactacagg cagtccggga acagaccgca aaacagcttc cagctccaga acttatagcc
     4741 caagcaacag aagcaaaggc gaaggcagaa agtctggcta cagaacgtgc caaagacctt
     4801 cagctccttc aggaagagaa caaccgattg cgtaacgaaa tcaaagttct tcaggagtat
     4861 aaggaaaaag cacaccggga actttgccgg gtacgtgatg aacagaaaac attcgggaaa
     4921 ataaaagtaa atacagaact gtagtttggg tatatgctta tatcatagga tgaacggcac
     4981 atttcaaaac attcttccgg gaactatgga acaagttaca gagagcatta ggacttaata
     5041 acggcagggg atttcattta tagcataggg aactgcgagt gagtattgta ataaaagcgg
     5101 taaaacatga aaaaacagct ataatacttc aaataaaact catgccttaa tataaaaaag
     5161 aggagagaac aataagtcct ctcctctttt ttatattaag gcatgaactg tattcagcac
     5221 tacaaactat ttacccacat acgaacagtt cttcttgctc tattttgttc accttctaaa
     5281 catttacgat cattcttatt ataaactatt tttagattac tatccaattg caatgtatca
     5341 tacaaaggaa gcataaagaa gttttcttca tcaagcagtt tttccattat taatggtata
     5401 atcacacttc ccatttcttg taaagatttg aattgaggta actgtttgta atccaaagta
     5461 gatgaactaa acattgtttt gggatttgta ttaatttcat ttttccatgc agcaattaaa
     5521 gactcgaatt tgctaacaat ttcgctatct acttttttac ttctttctaa tactttattc
     5581 ttctctaagt cggataaaac aatatcttct ttagagacct cttctgctac ttcttttaaa
     5641 tcagatattt tactgttctg ttttacaaaa gaaactaaaa tactaccata ttcagaacca
     5701 tttaattcag ttcttccatg agtgatgcga atatttggtc ctaattttga ctcccatgta
     5761 tttccactat aaataacact tccatgagtc attttataag tgttaagccc ccatccatca
     5821 acatctgcac cacttccttg agtcgatgtt tgttcataat ttgaactcgc tccgtataaa
     5881 gatttcgcat ttttatactg attaataaaa tcattataaa gctcttcagg attaatccac
     5941 ttactcgtta tacccataga atacgctata caattatatg tatacgtagc ttcaccagta
     6001 actgtaacat tatcggtctt taaattagga aatgcttttt tcaacatatc agcttcttgt
     6061 ggagtcaagc ttctcctcat tgaagcagaa cgagttaata cctcttcatt cgaaaatgat
     6121 tggtcatctt gcataacaat gtcatcttca ttagaacatg ctacaaagaa agaacataac
     6181 atcaatgaat aaaataactt tttcataact ttcaattttc aattaacaac cataaaatta
     6241 caagaaatta tgattcattg ttctcaaaat ctaatat
//
