LOCUS       NC_020989               7500 bp    DNA     circular CON 06-SEP-2013
DEFINITION  Chlamydia trachomatis Ia/SotonIa3 plasmid pSotonIa3, complete
            sequence.
ACCESSION   NC_020989
VERSION     NC_020989.1  GI:478475664
DBLINK      BioProject: PRJNA196781
KEYWORDS    complete replicon.
SOURCE      Chlamydia trachomatis Ia/SotonIa3
  ORGANISM  Chlamydia trachomatis Ia/SotonIa3
            Bacteria; Chlamydiae; Chlamydiales; Chlamydiaceae;
            Chlamydia/Chlamydophila group; Chlamydia.
REFERENCE   1
  AUTHORS   Harris,S.R., Clarke,I.N., Seth-Smith,H.M., Solomon,A.W.,
            Cutcliffe,L.T., Marsh,P., Skilton,R.J., Holland,M.J., Mabey,D.,
            Peeling,R.W., Lewis,D.A., Spratt,B.G., Unemo,M., Persson,K.,
            Bjartling,C., Brunham,R., de Vries,H.J., Morre,S.A.,
            Speksnijder,A., Bebear,C.M., Clerc,M., de Barbeyrac,B., Parkhill,J.
            and Thomson,N.R.
  TITLE     Whole-genome analysis of diverse Chlamydia trachomatis strains
            identifies phylogenetic relationships masked by current clinical
            typing
  JOURNAL   Nat. Genet. 44 (4), 413-419 (2012)
   PUBMED   22406642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7500)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2013) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 7500)
  AUTHORS   Seth-Smith,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2011) Wellcome Trust Sanger Institute, Pathogen
            Genomics, Wellcome Trust Genome Campus, Hinxton, CB10 1SA, UNITED
            KINGDOM
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence is identical to HE603237.
            Data release policy http://www.sanger.ac.uk/legal/#t_2.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7500
                     /organism="Chlamydia trachomatis Ia/SotonIa3"
                     /mol_type="genomic DNA"
                     /strain="Ia/SotonIa3"
                     /db_xref="taxon:1071767"
                     /plasmid="pSotonIa3"
     rep_origin      1..88
                     /note="putative origin of replication; contains 4 copies
                     of a 22bp repeat"
     gene            165..1082
                     /locus_tag="SOTONIa3_p1"
                     /db_xref="GeneID:16809195"
     CDS             165..1082
                     /locus_tag="SOTONIa3_p1"
                     /codon_start=1
                     /transl_table=11
                     /product="putative virulence plasmid integrase"
                     /protein_id="YP_007743404.1"
                     /db_xref="GI:478475665"
                     /db_xref="GOA:H8WQL2"
                     /db_xref="InterPro:IPR002104"
                     /db_xref="InterPro:IPR011010"
                     /db_xref="InterPro:IPR013762"
                     /db_xref="UniProtKB/TrEMBL:H8WQL2"
                     /db_xref="GeneID:16809195"
                     /translation="MGSMAFHKSRLFLTFGDASEIWLSTLSHLTRKNYASGINFLVSL
                     EILDLSETLIKAISLDHSESLFKIKSLDVFNGKVVSEASKQARAACYISFTKFLYRLT
                     KGYIKPAIPLKDFGNTTFFKIRDKIKTESISKQEWTVFFEALRIVNYRDYLIGKLIVQ
                     GIRKLDEILSLRTDDLFFASNQISFRIKKRQNKETKILITFPISLMEELQKYTCGRNG
                     RIFVSKIGIPVTTSQVAHNFRLAEFHSAMKIKITPRVLRASALIHLKQIGLKDEEIMR
                     ISCLSSRQSVCSYCSGEEVSPLVQTPTIL"
     misc_feature    177..977
                     /locus_tag="SOTONIa3_p1"
                     /note="Integrase [DNA replication, recombination, and
                     repair]; Region: XerC; COG0582"
                     /db_xref="CDD:223655"
     misc_feature    570..995
                     /locus_tag="SOTONIa3_p1"
                     /note="DNA breaking-rejoining enzymes,
                     intergrase/recombinases, C-terminal catalytic domain. The
                     tyrosine recombinase/integrase family share the same
                     catalytic domain containing six conserved active site
                     residues. The best-studied members of this diverse
                     family...; Region: INT_REC_C; cd01182"
                     /db_xref="CDD:238587"
     misc_feature    order(651..653,924..926,933..935)
                     /locus_tag="SOTONIa3_p1"
                     /note="active site"
                     /db_xref="CDD:238587"
     misc_feature    order(651..653,933..935)
                     /locus_tag="SOTONIa3_p1"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:238587"
     misc_feature    order(651..653,729..731,924..926,933..935)
                     /locus_tag="SOTONIa3_p1"
                     /note="Int/Topo IB signature motif; other site"
                     /db_xref="CDD:238587"
     misc_feature    order(651..653,657..659,729..731,921..926)
                     /locus_tag="SOTONIa3_p1"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238587"
     gene            complement(1133..2125)
                     /locus_tag="SOTONIa3_p2"
                     /db_xref="GeneID:16809199"
     CDS             complement(1133..2125)
                     /locus_tag="SOTONIa3_p2"
                     /codon_start=1
                     /transl_table=11
                     /product="putative virulence plasmid integrase"
                     /protein_id="YP_007743405.1"
                     /db_xref="GI:478475666"
                     /db_xref="GOA:H8WQL3"
                     /db_xref="InterPro:IPR002104"
                     /db_xref="InterPro:IPR011010"
                     /db_xref="InterPro:IPR013762"
                     /db_xref="UniProtKB/TrEMBL:H8WQL3"
                     /db_xref="GeneID:16809199"
                     /translation="MGKGILSLQQEMSLEYSEKSYQEVLKIRQESYWKRMKSFSLFEV
                     IMHWTASLNKHTCRSYRGSFLSLEKIGLLSLDMNLQEFSLLNHNLILDAIKKVSSAKT
                     SWTEGTKQVRAASYISLTRFLNRMTQGIVAIAQPSKQENSRTFFKTREIVKTDAMNSL
                     QTASFLKELKKINARDWLIAQTMLQGGKRSSEVLSLEISQICFQQATISFSQLKNRQT
                     EKRIIITYPQKFMHFLQEYIGQRRGFVFVTRSGKMVGLRQIARTFSQAGLQAAIPFKI
                     TPHVLRATAVTEYKRLGCSDSDIMKVTGHATAKMIFAYDKSSREDNASKKTALI"
     misc_feature    complement(1178..1645)
                     /locus_tag="SOTONIa3_p2"
                     /note="DNA breaking-rejoining enzymes,
                     intergrase/recombinases, C-terminal catalytic domain. The
                     tyrosine recombinase/integrase family share the same
                     catalytic domain containing six conserved active site
                     residues. The best-studied members of this diverse
                     family...; Region: INT_REC_C; cd01182"
                     /db_xref="CDD:238587"
     misc_feature    complement(order(1184..1186,1211..1213,1280..1282,
                     1289..1291,1559..1561))
                     /locus_tag="SOTONIa3_p2"
                     /note="active site"
                     /db_xref="CDD:238587"
     misc_feature    complement(order(1184..1186,1280..1282,1559..1561))
                     /locus_tag="SOTONIa3_p2"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:238587"
     misc_feature    complement(order(1184..1186,1289..1294,1484..1486,
                     1556..1561))
                     /locus_tag="SOTONIa3_p2"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238587"
     misc_feature    complement(order(1184..1186,1211..1213,1280..1282,
                     1289..1291,1484..1486,1559..1561))
                     /locus_tag="SOTONIa3_p2"
                     /note="Int/Topo IB signature motif; other site"
                     /db_xref="CDD:238587"
     gene            2224..3579
                     /locus_tag="SOTONIa3_p3"
                     /db_xref="GeneID:16809200"
     CDS             2224..3579
                     /locus_tag="SOTONIa3_p3"
                     /codon_start=1
                     /transl_table=11
                     /product="putative helicase, DnaB family"
                     /protein_id="YP_007743406.1"
                     /db_xref="GI:478475667"
                     /db_xref="GOA:H8WQL4"
                     /db_xref="InterPro:IPR003593"
                     /db_xref="InterPro:IPR007693"
                     /db_xref="InterPro:IPR007694"
                     /db_xref="UniProtKB/TrEMBL:H8WQL4"
                     /db_xref="GeneID:16809200"
                     /translation="MKTRSEIENRMQDIEYALLGKALIFEDSTEYILRQLANYEFKCS
                     HHKNIFIVFKYLKDNGLPITVDSAWEELLRRRIKDMDKSYLGLMLHDALSNDKLRSVS
                     HTVFLDDLSVCSAEENLSNFIFRSFNEYNENPLRRSPFLLLERIKGRLDSAIAKTFSI
                     RSARGRSIYDIFSQSEIGVLARIKKRRAAFSENQNSFFDGFPTGYKDIDDKGVILAKG
                     NFVIIAARPSIGKTALAIDMAINLAVTQQRRVGFLSLEMSAGQIVERIIANLTGISGE
                     KLQRGDLSKEELFRVEEAGETVRESHFYICSDSQYKLNLIANQIRLLRKEDRVDVIFI
                     DYLQLINSSVGENRQNEIADISRTLRGLASELNIPIVCLSQLSRKVEDRANKVPMLSD
                     LRDSGQIEQDADVILFINRKESSSNCEITVGKNRHGSVFSSVLHFDPKISKFSAIKKV
                     W"
     misc_feature    2242..2505
                     /locus_tag="SOTONIa3_p3"
                     /note="DnaB-like helicase N terminal domain; Region: DnaB;
                     pfam00772"
                     /db_xref="CDD:216111"
     misc_feature    2821..3570
                     /locus_tag="SOTONIa3_p3"
                     /note="DnaB-like helicase C terminal domain; Region:
                     DnaB_C; pfam03796"
                     /db_xref="CDD:217733"
     misc_feature    2839..3540
                     /locus_tag="SOTONIa3_p3"
                     /note="DnaB helicase C terminal domain. The hexameric
                     helicase DnaB unwinds the DNA duplex at the  chromosome
                     replication fork. Although the mechanism by which DnaB
                     both couples ATP hydrolysis to translocation along DNA and
                     denatures the duplex is unknown, a...; Region: DnaB_C;
                     cd00984"
                     /db_xref="CDD:238484"
     misc_feature    2899..2919
                     /locus_tag="SOTONIa3_p3"
                     /note="Walker A motif; other site"
                     /db_xref="CDD:238484"
     misc_feature    order(2914..2919,3226..3228,3346..3348,3454..3456,
                     3499..3501,3532..3534)
                     /locus_tag="SOTONIa3_p3"
                     /note="ATP binding site [chemical binding]; other site"
                     /db_xref="CDD:238484"
     misc_feature    3217..3228
                     /locus_tag="SOTONIa3_p3"
                     /note="Walker B motif; other site"
                     /db_xref="CDD:238484"
     misc_feature    order(3241..3246,3262..3270,3343..3369,3451..3456,
                     3469..3474)
                     /locus_tag="SOTONIa3_p3"
                     /note="DNA binding loops [nucleotide binding]"
                     /db_xref="CDD:238484"
     gene            3573..4625
                     /locus_tag="SOTONIa3_p4"
                     /db_xref="GeneID:16809196"
     CDS             3573..4625
                     /locus_tag="SOTONIa3_p4"
                     /codon_start=1
                     /transl_table=11
                     /product="conserved hypothetical virulence plasmid
                     protein"
                     /protein_id="YP_007743407.1"
                     /db_xref="GI:478475668"
                     /db_xref="UniProtKB/TrEMBL:H8WQL5"
                     /db_xref="GeneID:16809196"
                     /translation="MVNYSNCHFIKSPIHLENQKFGRRPGQSIKISPKLAQNGMVEVI
                     GLDFLSSHYHALAAIQRLLTATNYKGNTKGVVLSRESNSFQFEGWIPRIRFTKTEFLE
                     AYGVKRYKTSRNKYEFSGKEAETALEALYHLGHQPFLIVATRTRWTNGTQIVDRYQTL
                     SPIIRIYEGWEGLTDEENIDIDLTPFNSPSTRKHKGFVVEPCPILVDQIESYFVIKPA
                     NVYQEIKMRFPNASKYAYTFIDWVITAAAKKRRKLTKDNSWPENLLLNVNVKSLAYIL
                     RMNRYICTRNWKKIELAIDKCIEIAIQLGWLSRRKRIEFLDSSKLSKKEILYLNKERF
                     EEITKKSKEQMEQLEQ"
     gene            4699..5493
                     /locus_tag="SOTONIa3_p5"
                     /db_xref="GeneID:16809194"
     CDS             4699..5493
                     /locus_tag="SOTONIa3_p5"
                     /codon_start=1
                     /transl_table=11
                     /product="conserved hypothetical virulence plasmid
                     protein"
                     /protein_id="YP_007743408.1"
                     /db_xref="GI:478475669"
                     /db_xref="InterPro:IPR008444"
                     /db_xref="UniProtKB/TrEMBL:H8WQK8"
                     /db_xref="GeneID:16809194"
                     /translation="MGNSGFYLYNTENCVFADNIKVGQMTEPLKDQQIILGTTSTPVA
                     AKMTASDGISLTVSNNSSTNASITIGLDAEKAYQLILEKLGNQILDGIADTIVDSTVQ
                     DILDKITTDPSLGLLKAFNNFPITNKIQCNGLFTPSNIETLLGGTEIGKFTVTPKSSG
                     SMFLVSADIIASRMEGSVVLALVREGDSKPCAISYGYSSGVPNLCSLRTSITNTGLTP
                     TTYSLRVGGLESGVVWVNALSNGNDILGITNTSNVSFLEVIPQTNA"
     misc_feature    4699..5490
                     /locus_tag="SOTONIa3_p5"
                     /note="Chlamydia virulence protein PGP3-D; Region:
                     Chlam_vir; pfam05475"
                     /db_xref="CDD:147581"
     gene            5563..5871
                     /locus_tag="SOTONIa3_p6"
                     /db_xref="GeneID:16809198"
     CDS             5563..5871
                     /locus_tag="SOTONIa3_p6"
                     /codon_start=1
                     /transl_table=11
                     /product="conserved hypothetical virulence plasmid
                     protein"
                     /protein_id="YP_007743409.1"
                     /db_xref="GI:478475670"
                     /db_xref="UniProtKB/TrEMBL:C3W7Z0"
                     /db_xref="GeneID:16809198"
                     /translation="MQNKRKVRDDFIKIVKDVKKDFPELDLKIRVNKEKVTFLNSPLE
                     LYHKSVSLILGLLQQIENSLGLFPDSPVLEKLEDNSLKLKKALIMLILSRKDMFSKAE
                     "
     gene            5899..6693
                     /locus_tag="SOTONIa3_p7"
                     /db_xref="GeneID:16809193"
     CDS             5899..6693
                     /locus_tag="SOTONIa3_p7"
                     /codon_start=1
                     /transl_table=11
                     /product="putative plasmid partitioning protein, ParA
                     family"
                     /protein_id="YP_007743410.1"
                     /db_xref="GI:478475671"
                     /db_xref="InterPro:IPR002586"
                     /db_xref="UniProtKB/TrEMBL:H8WQ66"
                     /db_xref="GeneID:16809193"
                     /translation="MHTLVFCSFKGGTGKTTLSLNVGCNLAQFLGKKVLLADLDPQSN
                     LSSGLGASVRSNQKGLHDIVYTSNDLKSIICETKKDSVDLIPASFLSEQFRELDIHRG
                     PSNNLKLFLNEYCAPFYDICIIDTPPSLGGLTKEAFVAGDKLIACLTPEPFSILGLQK
                     IREFLSSVGKPEEEHILGIALSFWDDRNSTNQMYIDIIESIYKNKLFSTKIRRDISLS
                     RSLLKEDSVANVYPNSRAAEDILKLTHEIANILHIEYERDYSQRTT"
     misc_feature    5899..6666
                     /locus_tag="SOTONIa3_p7"
                     /note="ATPases involved in chromosome partitioning [Cell
                     division and chromosome partitioning]; Region: Soj;
                     COG1192"
                     /db_xref="CDD:224113"
     misc_feature    5905..>6024
                     /locus_tag="SOTONIa3_p7"
                     /note="ParA and ParB of Caulobacter crescentus belong to a
                     conserved family of bacterial proteins implicated in
                     chromosome segregation. ParB binds to DNA sequences
                     adjacent to the origin of replication and localizes to
                     opposite cell poles shortly following the...; Region:
                     ParA; cd02042"
                     /db_xref="CDD:238997"
     misc_feature    5926..5946
                     /locus_tag="SOTONIa3_p7"
                     /note="P-loop; other site"
                     /db_xref="CDD:238997"
     misc_feature    5944..5946
                     /locus_tag="SOTONIa3_p7"
                     /note="Magnesium ion binding site [ion binding]; other
                     site"
                     /db_xref="CDD:238997"
     misc_feature    <6253..6393
                     /locus_tag="SOTONIa3_p7"
                     /note="ParA and ParB of Caulobacter crescentus belong to a
                     conserved family of bacterial proteins implicated in
                     chromosome segregation. ParB binds to DNA sequences
                     adjacent to the origin of replication and localizes to
                     opposite cell poles shortly following the...; Region:
                     ParA; cd02042"
                     /db_xref="CDD:238997"
     misc_feature    6271..6273
                     /locus_tag="SOTONIa3_p7"
                     /note="Magnesium ion binding site [ion binding]; other
                     site"
                     /db_xref="CDD:238997"
     gene            6690..7433
                     /locus_tag="SOTONIa3_p8"
                     /db_xref="GeneID:16809197"
     CDS             6690..7433
                     /locus_tag="SOTONIa3_p8"
                     /codon_start=1
                     /transl_table=11
                     /product="conserved hypothetical virulence plasmid
                     protein"
                     /protein_id="YP_007743411.1"
                     /db_xref="GI:478475672"
                     /db_xref="InterPro:IPR005350"
                     /db_xref="UniProtKB/TrEMBL:C3W7Z2"
                     /db_xref="GeneID:16809197"
                     /translation="MNKLKKEADVFFKKNQTAASLDFKKTLPSIELFSATLNSEESQS
                     LDRLFLSESQNYSDEEFYQEDILAVKLLTGQIKSIQKQHVLLLGEKIYNARKILSKDH
                     FSSTTFSSWIELVFRTKSSAYNALAYYELFINLPNQTLQKEFQSIPYKSAYILAARKG
                     DLKTKVDVIGKVCGMSNSSAIRVLDQFLPSSRNKDVRETIDKSDSEKNRQLSDFLIEI
                     LRIMCSGVSLSSYNENLLQQLFELFKQKS"
     misc_feature    6690..7382
                     /locus_tag="SOTONIa3_p8"
                     /note="Uncharacterized protein family (UPF0137); Region:
                     UPF0137; pfam03677"
                     /db_xref="CDD:146355"
ORIGIN      
        1 tttgcaactc ttggtggtag actttgcaac tcttggtggt agactttgca actcttggtg
       61 gtagactttg caactcttgg tggtagactt ggtcataatg gacttttgtt aaaaaatttc
      121 ttaaaatctt agagctccga ttttgaatag ctttggttaa gaaaatgggc tcgatggctt
      181 tccataaaag tagattgttc ttaacttttg gggacgcgtc ggaaatttgg ttatctactt
      241 tatctcatct aactagaaaa aattatgcgt ctgggattaa ctttcttgtt tctttagaga
      301 ttctggattt atcagaaacc ttgataaagg ctatttctct tgaccacagc gaatctttgt
      361 ttaaaatcaa gtctctagat gtttttaatg gaaaagtcgt ttcagaggcc tctaaacagg
      421 ctagagcggc atgctacata tctttcacaa agtttttgta tagattgacc aagggatata
      481 ttaaacccgc tattccattg aaagattttg gaaacactac attttttaaa atccgagaca
      541 aaatcaaaac agaatcgatt tctaagcagg aatggacagt tttttttgaa gcgctccgga
      601 tagtgaatta tagagactat ttaatcggta aattgattgt acaagggatc cgtaagttag
      661 acgaaatttt gtctttgcgc acagacgatc tattttttgc atccaatcag atttcctttc
      721 gcattaaaaa aagacagaat aaagaaacca aaattctaat cacatttcct atcagcttaa
      781 tggaagagtt gcaaaaatac acttgtggga gaaatgggag aatatttgtt tctaaaatag
      841 ggattcctgt aacaacaagt caggttgcgc ataattttag gcttgcagag ttccatagtg
      901 ctatgaaaat aaaaattact cccagagtac ttcgtgcaag cgctttgatt catttaaagc
      961 aaataggatt aaaagatgag gaaatcatgc gtatttcctg tctttcatcg agacaaagtg
     1021 tgtgttctta ttgttctggg gaagaggtaa gtcctctagt acaaacaccc acaatattgt
     1081 gatataatta aaattatatt catattctgt tgccagaaaa aacaccttta ggctatatta
     1141 gagccgtctt ctttgaagcg ttgtcttctc gagaagattt atcgtacgca aatatcatct
     1201 ttgcggttgc gtgtcctgtg accttcatta tgtcggagtc tgagcaccct aggcgtttgt
     1261 actccgtcac agcggttgct cgaagcacgt gcggggttat cttaaaaggg attgcagctt
     1321 gtagtcctgc ttgagagaac gtgcgggcga tttgccttaa ccccaccatt tttccggagc
     1381 gagttacgaa gacaaaacct cttcgttgac cgatgtactc ttgtagaaag tgcataaact
     1441 tctgaggata agttataata atcctctttt ctgtctgacg gttcttaagc tgggagaaag
     1501 aaatggtagc ttgttggaaa caaatctgac taatctccaa gcttaagact tcagaggagc
     1561 gtttgcctcc ttggagcatt gtctgggcga tcaaccaatc ccgggcattg atttttttta
     1621 gctcttttag gaaggatgct gtttgcaaac tgttcatcgc atccgttttt actatttccc
     1681 tggttttaaa aaatgttcga ctattttctt gtttagaagg ttgcgctata gcgactattc
     1741 cttgagtcat cctgtttagg aatcttgtta aggaaatata gcttgctgct cgaacttgtt
     1801 tagtaccttc ggtccaggaa gtcttggcag aggaaacttt tttaatcgca tctaggatta
     1861 gattatgatt taaaagggaa aactcttgca gattcatatc caaggacaat agaccaatct
     1921 tttctaaaga caaaaaagat cctcgatatg atctacaagt atgtttgttg agtgatgcgg
     1981 tccaatgcat aataacttcg aataaggaga agcttttcat gcgtttccaa taggattctt
     2041 ggcgaatttt taaaacttcc tgataagact tttcgctata ttctaacgac atttcttgct
     2101 gcaaagataa aatcccttta cccatgaaat ccctcgtagt ataacctatc cgtaaaatgt
     2161 cctgattatt tcactaatca ggttgttaac aggatagcac gctcggtatt tttttatata
     2221 aacatgaaaa ctcgttccga aatagaaaat cgcatgcaag atatcgagta tgcgttgtta
     2281 ggtaaagctc tgatatttga agactctact gagtatattc tgaggcagct tgctaattat
     2341 gagtttaagt gttctcatca taaaaacata ttcatagtat ttaaatactt aaaagacaat
     2401 ggattaccta taactgtaga ctcggcttgg gaagagcttt tgcggcgtcg tatcaaagat
     2461 atggacaaat cgtatctcgg gttaatgttg catgatgctt tatcaaatga caagcttaga
     2521 tccgtttctc atacggtttt cctcgatgat ttgagcgtgt gtagcgctga agaaaatttg
     2581 agtaatttca ttttccgctc gtttaatgag tacaatgaaa atccattgcg tagatctccg
     2641 tttctattgc ttgagcgtat aaagggaagg cttgatagtg ctatagcaaa gactttttct
     2701 attcgcagcg ctagaggccg gtctatttat gatatattct cacagtcaga aattggagtg
     2761 ctggctcgta taaaaaaaag acgagcagcg ttctctgaga atcaaaattc tttctttgat
     2821 ggcttcccaa caggatacaa ggatattgat gataaaggag ttatcttagc taaaggtaat
     2881 ttcgtgatta tagcagctag gccatctata gggaaaacag ctttagctat agacatggcg
     2941 ataaatcttg cggttactca acagcgtaga gttggtttcc tatctctaga aatgagcgca
     3001 ggtcaaattg ttgagcggat tattgctaat ttaacaggaa tatctggtga aaaattacaa
     3061 agaggggatc tctctaaaga agaattattc cgagtggaag aagctggaga aacagttaga
     3121 gaatcacatt tttatatctg cagtgatagt cagtataagc ttaatttaat cgcgaatcag
     3181 atccggttgc tgagaaaaga agatcgagta gacgtaatat ttatcgatta cttgcagttg
     3241 atcaactcat cggttggaga aaatcgtcaa aatgaaatag cagatatatc tagaacctta
     3301 agaggtttag cctcagagct aaacattcct atagtttgtt tatcccaact atctagaaaa
     3361 gttgaggata gagcaaataa agttcccatg ctttcagatt tgcgagacag cggtcaaata
     3421 gagcaagacg cagatgtgat tttgtttatc aataggaagg aatcgtcttc taattgtgag
     3481 ataactgttg ggaaaaatag acatggatcg gttttctctt cggtattaca tttcgatcca
     3541 aaaattagta aattctccgc tattaaaaaa gtatggtaaa ttatagtaac tgccacttca
     3601 tcaaaagtcc tatccacctt gaaaatcaga agtttggaag aagacctggt caatctatta
     3661 agatatctcc caaattggct caaaatggga tggtagaagt tataggtctt gattttcttt
     3721 catctcatta ccatgcatta gcagctatcc aaagattact gactgcaacg aattacaagg
     3781 ggaacacaaa aggggttgtt ttatccagag aatcaaatag ttttcaattt gaaggatgga
     3841 taccaagaat ccgttttaca aaaactgaat tcttagaggc ttatggagtt aagcggtata
     3901 aaacatccag aaataagtat gagtttagtg gaaaagaagc tgaaactgct ttagaagcct
     3961 tgtaccattt aggacatcaa ccgtttttaa tagtggcaac tagaactcga tggactaatg
     4021 gaacacaaat agtagaccgt taccaaactc tttctccgat cattaggatt tacgaaggat
     4081 gggaaggttt aactgacgaa gaaaatatag atatagactt aacacctttt aattcaccat
     4141 ctacacggaa acataaaggg ttcgttgtag agccatgtcc tatcttggta gatcaaatag
     4201 aatcctactt tgtaatcaag cctgcaaatg tataccaaga aataaaaatg cgtttcccaa
     4261 acgcatcaaa gtatgcttac acatttatcg actgggtgat tacagcagct gcgaaaaaga
     4321 gacgaaaatt aactaaggat aattcttggc cagaaaactt gttattaaac gttaacgtta
     4381 aaagtcttgc atatatttta aggatgaatc ggtacatctg tacaaggaac tggaaaaaaa
     4441 tcgagttagc tatcgataaa tgtatagaaa tcgccattca gcttggctgg ttatctagaa
     4501 gaaaacgcat tgaatttctg gattcttcta aactctctaa aaaagaaatt ctatatctaa
     4561 ataaagagcg ctttgaagaa ataactaaga aatctaaaga acaaatggaa caattagaac
     4621 aataatctat taattaatag caggcttgaa actaaaaacc taatttattt aaagctcaaa
     4681 ataaaaaaga gttttaaaat gggaaattct ggtttttatt tgtataacac tgaaaactgc
     4741 gtctttgctg ataatatcaa agttgggcaa atgacagagc cgctcaagga ccagcaaata
     4801 atccttggga caacatcaac acctgtcgca gccaaaatga cagcttctga tggaatatct
     4861 ttaacagtct ccaataattc atcaaccaat gcttctatta caattggttt ggatgcggaa
     4921 aaagcttacc agcttattct agaaaagttg ggaaatcaaa ttcttgatgg aattgctgat
     4981 actattgttg atagtacagt ccaagatatt ttagacaaaa tcacaacaga cccttctcta
     5041 ggtttgttga aagcttttaa caactttcca atcactaata aaattcaatg caacgggtta
     5101 ttcactccca gtaacattga aactttatta ggaggaactg aaataggaaa attcacagtc
     5161 acacccaaaa gctctgggag catgttctta gtctcagcag atattattgc atcaagaatg
     5221 gaaggcagcg ttgttctagc tttggtacga gaaggtgatt ctaagccctg cgcgattagt
     5281 tatggatact catcaggcgt tcctaattta tgtagtctaa gaaccagcat tactaataca
     5341 ggattgactc cgacaacgta ttcattacgt gtaggcggtt tagaaagcgg tgtggtatgg
     5401 gttaatgccc tttctaatgg caatgatatt ttaggaataa caaatacttc taatgtatct
     5461 tttttggagg taatacctca aacaaacgct taaacaattt ttattggatt tttcttatag
     5521 gttttatatt tagagaaaac agttcgaatt acggggtttg ttatgcaaaa taaaagaaaa
     5581 gtgagggacg attttattaa aattgttaaa gatgtgaaaa aagatttccc cgaattagac
     5641 ctaaaaatac gagtaaacaa ggaaaaagta actttcttaa attctccctt agaactctac
     5701 cataaaagtg tctcactaat tctaggactg cttcaacaaa tagaaaactc tttaggatta
     5761 ttcccagact ctcctgttct tgaaaaatta gaggataaca gtttaaagct aaaaaaggct
     5821 ttgattatgc ttatcttgtc tagaaaagac atgttttcca aggctgaata gacaacttac
     5881 tctaacgttg gagttgattt gcacacctta gttttttgct cttttaaggg gggaactgga
     5941 aaaacaacac tttctctaaa cgtgggatgc aacttggccc aatttttagg gaaaaaagtg
     6001 ttacttgctg acctagaccc acaatccaat ttatcttctg gattgggggc tagtgtcaga
     6061 agtaaccaaa aaggcttgca cgacatagta tacacatcaa acgatttaaa atcaatcatt
     6121 tgcgaaacaa aaaaagatag tgtggaccta attcctgcat catttttatc tgaacagttt
     6181 agagaattgg atattcatag aggacctagt aacaacttaa agttatttct gaatgagtac
     6241 tgcgctcctt tttatgacat ctgcataata gacactccac ctagcctagg agggttaacg
     6301 aaagaagctt ttgttgcagg agacaaatta attgcttgtt taactccaga acctttttct
     6361 attctagggt tacaaaagat acgtgaattc ttaagttcgg tcggaaaacc tgaagaagaa
     6421 cacattcttg gaatagcttt gtctttttgg gatgatcgta actcgactaa ccaaatgtat
     6481 atagacatta tcgagtctat ttacaaaaac aagctttttt caacaaaaat tcgtcgagat
     6541 atttctctta gccgttctct tcttaaagaa gattctgtag ctaatgtcta tccaaattct
     6601 agggccgcag aagatattct gaagttaacg catgaaatag caaatatttt gcatatcgaa
     6661 tatgaacgag attactctca gaggacaacg tgaacaaact aaaaaaagaa gcggatgttt
     6721 tttttaaaaa aaatcaaact gccgcttctc tagattttaa gaagacactt ccttccattg
     6781 aactattctc agcaactttg aattctgagg aaagtcagag tttggatcga ttatttttgt
     6841 cagagtccca aaactattcg gatgaagaat tttatcaaga agacatccta gcggtaaaac
     6901 tgcttactgg tcagataaaa tccatacaga agcaacacgt acttctttta ggagaaaaaa
     6961 tctataatgc tagaaaaatc ctgagtaagg atcacttctc ctcgacaact ttttcatctt
     7021 ggatagagtt agtttttaga actaagtctt ctgcttacaa tgctcttgca tattacgagc
     7081 tttttataaa cctccccaac caaactctac aaaaagagtt tcaatcgatc ccctataaat
     7141 ccgcatatat tttggccgct agaaaaggcg atttaaaaac caaggtcgat gtgataggga
     7201 aagtatgtgg aatgtcgaac tcatcggcga taagggtgtt ggatcaattt cttccttcat
     7261 ctagaaacaa agacgttaga gaaacgatag ataagtctga ttcagagaag aatcgccaat
     7321 tatctgattt cttaatagag atacttcgca tcatgtgttc cggagtttct ttgtcctcct
     7381 ataacgaaaa tcttctacaa cagctttttg aactttttaa gcaaaagagc tgatcctccg
     7441 tcagctcata tatatatcta ttatatatat atatttaggg atttgatttt acgagagagc
//
