LOCUS       NC_012790              10287 bp    DNA     circular CON 08-JUN-2013
DEFINITION  Geobacillus sp. WCH70 plasmid pWCH7002, complete sequence.
ACCESSION   NC_012790
VERSION     NC_012790.1  GI:239812973
DBLINK      Project: 59045
            BioProject: PRJNA59045
KEYWORDS    .
SOURCE      Geobacillus sp. WCH70
  ORGANISM  Geobacillus sp. WCH70
            Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae;
            Geobacillus.
REFERENCE   1  (bases 1 to 10287)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
            Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Brettin,T.,
            Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N.,
            Mikhailova,N., Brumm,P., Mead,D.A. and Richardson,P.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Complete sequence of plasmid 2 of Geopacillus sp. WCH70
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10287)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUN-2009) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 10287)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
            Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Brettin,T.,
            Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N.,
            Mikhailova,N., Brumm,P., Mead,D.A. and Richardson,P.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUN-2009) US DOE Joint Genome Institute, 2800
            Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CP001640.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 4082513
            Source DNA and bacteria available from David A. Mead
            (dmead@lucigen.com)
            Contacts: David A. Mead (dmead@lucigen.com)
                      David Bruce (microbe@cuba.jgi-psf.org)
            Annotation done by JGI-ORNL and JGI-PGF
            Finishing done by JGI-LANL
            Finished microbial genomes have been curated to close all gaps with
            greater than 98% coverage of at least two independent clones. Each
            base pair has a minimum q (quality) value of 30 and the total error
            rate is less than one per 50000.
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. it is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            
            ##Metadata-START##
            Organism Display Name :: Geobacillus sp. WCH70
            GOLD Stamp ID         :: Gi02009
            Oxygen Requirement    :: Facultative
            Cell Shape            :: Rod-shaped
            Motility              :: Motile
            Sporulation           :: Sporulating
            Temperature Range     :: Thermophile
            Biotic Relationship   :: Free living
            Diseases              :: None
            Energy Source         :: Chemoorganotroph
            ##Metadata-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10287
                     /organism="Geobacillus sp. WCH70"
                     /mol_type="genomic DNA"
                     /strain="WCH70"
                     /db_xref="taxon:471223"
                     /plasmid="pWCH7002"
     gene            62..214
                     /locus_tag="GWCH70_3469"
                     /db_xref="GeneID:7969495"
     CDS             62..214
                     /locus_tag="GWCH70_3469"
                     /inference="ab initio prediction:Prodigal:1.4"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_002941921.1"
                     /db_xref="GI:239812974"
                     /db_xref="GeneID:7969495"
                     /translation="MQRGNWNTGQGGKEDISEGGDGTNTTQSNCVVLRIRGKTLFLQR
                     FSFFLK"
     gene            276..824
                     /locus_tag="GWCH70_3470"
                     /db_xref="GeneID:7969487"
     CDS             276..824
                     /locus_tag="GWCH70_3470"
                     /inference="protein motif:PFAM:PF00589"
                     /note="PFAM: integrase family protein;
                     KEGG: bay:RBAM_018520 phage integrase family site specific
                     recombinase"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="YP_002941922.1"
                     /db_xref="GI:239812975"
                     /db_xref="InterPro:IPR002104"
                     /db_xref="GeneID:7969487"
                     /translation="MNRTQPIKDLAVIEKLGRYLKKTNTRDYMLFRCMLYFALRTQDI
                     LSLKVKDVRGKYQIYFKEQKTGKTKIIEIHPDLRPELEEYTQNMADDDWLFPSRIKGR
                     PLSREQAFRRLQAAGKKCGLPSLSGHVLRKTFCYHAYKNGVPLHILSELLNHSSEAVT
                     RRYIGLQQEDIKRAYFSVQFNC"
     misc_feature    288..815
                     /locus_tag="GWCH70_3470"
                     /note="DNA breaking-rejoining enzymes, C-terminal
                     catalytic domain. The DNA breaking-rejoining enzyme
                     superfamily includes type IB topoisomerases and tyrosine
                     recombinases that share the same fold in their catalytic
                     domain containing six conserved active site...; Region:
                     DNA_BRE_C; cl00213"
                     /db_xref="CDD:241691"
     misc_feature    order(393..395,657..659,666..668,735..737,762..764)
                     /locus_tag="GWCH70_3470"
                     /note="active site"
                     /db_xref="CDD:238231"
     misc_feature    order(393..398,465..467,651..659,762..764)
                     /locus_tag="GWCH70_3470"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238231"
     misc_feature    order(393..395,465..467,657..659,666..668,735..737,
                     762..764)
                     /locus_tag="GWCH70_3470"
                     /note="Int/Topo IB signature motif; other site"
                     /db_xref="CDD:238231"
     gene            858..2204
                     /locus_tag="GWCH70_3471"
                     /db_xref="GeneID:7969488"
     CDS             858..2204
                     /locus_tag="GWCH70_3471"
                     /inference="similar to AA sequence:KEGG:GKP04"
                     /note="KEGG: gka:GKP04 replication protein"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="YP_002941923.1"
                     /db_xref="GI:239812976"
                     /db_xref="GeneID:7969488"
                     /translation="MALQEYKYSQNPCEFRFEMDLYTYLKKLYKTKLKNAYFFICENE
                     NKNWVQKTYKSTEIDKAFADIVHSNAIDVYGSTCLHSSAKRVTENVIQIPALLVDLDY
                     YKTPYRDLTPKELVKKLEEVYFKPQKIPYPNAIISSGNGVYLIWFLKFTPGTDGTLLK
                     RRVIVKILYEMLKEFGADAKALDAAHVFRLPNTYNSKVTDRHKREVKAFVNELDDYTL
                     SKLSKKLPSLWEVWKKKHGIRTEQEKRRKGKIISVPQFKTRTLAYDHIKSILQLIELR
                     KGECTGYREMMLFFLRDRYHTMHAKRFYQQDETLFAESYAFIKEVNQKFTKPLSDKEI
                     RTNTLNKTKLYKFKTKTIIEWFDITLEEQIQLKVKTREAKNEKSKRQMRELRRKRGAL
                     TRREYLKQQHSITDDKLFKLKELLERYPNAKRKELAEMLGVSVYRVDQLKRELKSL"
     gene            2912..4024
                     /locus_tag="GWCH70_3472"
                     /db_xref="GeneID:7969489"
     CDS             2912..4024
                     /locus_tag="GWCH70_3472"
                     /inference="protein motif:TFAM:TIGR00675"
                     /note="TIGRFAM: DNA-cytosine methyltransferase;
                     PFAM: C-5 cytosine-specific DNA methylase;
                     KEGG: bca:BCE_4605 DNA-cytosine methyltransferase family
                     protein"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="YP_002941924.1"
                     /db_xref="GI:239812977"
                     /db_xref="InterPro:IPR001525"
                     /db_xref="GeneID:7969489"
                     /translation="MANAISFFAGAGGLDLGIKRAGFNILLSVEIEETYCQTLRQNFE
                     GLNVVCGDIMEYSRERVYQEAGLAEHEEVDLMIGGSPCQSFSTAGKRQAFADPRGQAM
                     LKYADLVNEVRPRFFLLENVKGLLSAALKHRPLNQRGPGHPPLTEEEQPGSALEYLLE
                     RFGDYDVKIKLINAADYGVPQKRERVFIIGVRNDLNIQYEFPEPTHNEDGSDGKLPWV
                     TVGEVLENLQVEEHHFIPYSKERLRFMKLIPKGGGNWRDLRAYGEEVVREAMGGAYYS
                     GGGKVGFFRRIDSNKPSPTLLTSPAQKSTNLGHPFEDRPLSIEEYLAIQGFPPGFRVA
                     GTLMQQYTQIGNAVPVPLAEILGASILERLRTLQTV"
     misc_feature    2912..4006
                     /locus_tag="GWCH70_3472"
                     /note="Site-specific DNA methylase [DNA replication,
                     recombination, and repair]; Region: Dcm; COG0270"
                     /db_xref="CDD:223348"
     misc_feature    2924..>3538
                     /locus_tag="GWCH70_3472"
                     /note="Cytosine-C5 specific DNA methylases; Methyl
                     transfer reactions play an important role in many aspects
                     of biology. Cytosine-specific DNA methylases are found
                     both in prokaryotes and eukaryotes. DNA methylation, or
                     the covalent addition of a methyl group...; Region:
                     Cyt_C5_DNA_methylase; cd00315"
                     /db_xref="CDD:238192"
     misc_feature    order(2933..2950,2996..3007,3062..3070,3086..3088,
                     3146..3148,3152..3154)
                     /locus_tag="GWCH70_3472"
                     /note="cofactor binding site; other site"
                     /db_xref="CDD:238192"
     misc_feature    order(3146..3148,3155..3160,3167..3178,3185..3187,
                     3203..3205,3269..3271,3275..3280,3458..3460,3464..3466)
                     /locus_tag="GWCH70_3472"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238192"
     misc_feature    order(3146..3148,3155..3157,3167..3169,3269..3271,
                     3275..3277,3458..3460,3464..3466)
                     /locus_tag="GWCH70_3472"
                     /note="substrate interaction site [chemical binding];
                     other site"
                     /db_xref="CDD:238192"
     misc_feature    <3758..3997
                     /locus_tag="GWCH70_3472"
                     /note="Cytosine-C5 specific DNA methylases; Methyl
                     transfer reactions play an important role in many aspects
                     of biology. Cytosine-specific DNA methylases are found
                     both in prokaryotes and eukaryotes. DNA methylation, or
                     the covalent addition of a methyl group...; Region:
                     Cyt_C5_DNA_methylase; cd00315"
                     /db_xref="CDD:238192"
     gene            4105..4911
                     /locus_tag="GWCH70_3473"
                     /db_xref="GeneID:7969490"
     CDS             4105..4911
                     /locus_tag="GWCH70_3473"
                     /inference="similar to AA sequence:KEGG:BCE_4604"
                     /note="KEGG: bca:BCE_4604 type II restriction
                     endonuclease, putative"
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease, putative"
                     /protein_id="YP_002941925.1"
                     /db_xref="GI:239812978"
                     /db_xref="GeneID:7969490"
                     /translation="MDEKTKERVRQHVKAVMERLIAKRTVEEPFSAKEIEEKNPFGYL
                     LVPIEVWKGSKMERSFVTTLGQGIFEQLGKIIAEGTGAFAANQYDKTLTINTFRTEKI
                     DSIIKKQRSKPKKGEKRVPPDLSKELDDIARLENEKYEIITVKSDLYIRRPDGKEEYY
                     SLKTVKPNLDQTAEAKKNLLLLRTGDPNCEAYFALPYNPAGEGNPYSKAGHSIPNRLF
                     NMDDERFVLIGSALWNKIGNDENTYEELLEIFREVGKESAERIRKEYFGL"
     misc_feature    4117..4866
                     /locus_tag="GWCH70_3473"
                     /note="MjaII restriction endonuclease; Region: RE_MjaII;
                     pfam09520"
                     /db_xref="CDD:150256"
     gene            5834..6202
                     /locus_tag="GWCH70_3474"
                     /db_xref="GeneID:7969491"
     CDS             5834..6202
                     /locus_tag="GWCH70_3474"
                     /inference="ab initio prediction:Prodigal:1.4"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_002941926.1"
                     /db_xref="GI:239812979"
                     /db_xref="GeneID:7969491"
                     /translation="MYFVNAEHKRNYDYLMGLYRLEPGQNVEYEASIYIAAYPSVFAC
                     FGKTISTEFGPLAYLSDEELAEEFGHNFGALTGTTRRMVACGMSLYNGLEVSLSDVFS
                     SLGEEEFNVLIQAIKIRAKR"
     misc_feature    5996..>6079
                     /locus_tag="GWCH70_3474"
                     /note="LEM (Lap2/Emerin/Man1) domain found in emerin,
                     lamina-associated polypeptide 2 (LAP2), inner nuclear
                     membrane protein Man1 and similar proteins; Region: LEM;
                     cl02649"
                     /db_xref="CDD:243125"
     misc_feature    order(6044..6061,6065..6070,6077..6079)
                     /locus_tag="GWCH70_3474"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]; other site"
                     /db_xref="CDD:240585"
     gene            7609..8322
                     /locus_tag="GWCH70_3475"
                     /db_xref="GeneID:7969492"
     CDS             7609..8322
                     /locus_tag="GWCH70_3475"
                     /inference="ab initio prediction:Prodigal:1.4"
                     /note="KEGG: hypothetical protein"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_002941927.1"
                     /db_xref="GI:239812980"
                     /db_xref="GeneID:7969492"
                     /translation="MCTKYLCKRWQGLLEAIINFAGQGYVYYHVTYLPAKKLNKADKI
                     DQKILQKYNLINDTKDKRYHRKKRGLANFRYLRWEHIIVILHTEGTIIQRGQEEKHLT
                     RFKLEQAKKHAVTYDDSFSDIRVKPMVLAISEQLELEVRYLPKKNVGQKLTVRLTKKM
                     FRDKKAELAALAEKRAKDKIVYVFNALNNLPAWRGIIIQKRQLYRFVMTQARKHNFTF
                     QKGELMVGDYRPKEKDIFV"
     gene            9118..9528
                     /locus_tag="GWCH70_3476"
                     /db_xref="GeneID:7969493"
     CDS             9118..9528
                     /locus_tag="GWCH70_3476"
                     /inference="ab initio prediction:Prodigal:1.4"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_002941928.1"
                     /db_xref="GI:239812981"
                     /db_xref="GeneID:7969493"
                     /translation="MIRMEEISISIPKEVLEKVEKSMGGWDDRNRVITNHLVYFYRLL
                     ETEKRTLTKIFSEEELLCIYAAFNGTIVDFRMTPGMFKATVRDALKYGELDFLISEHR
                     IDAGAFLQKIDGLSDLQTFVLLVDAQEYWAEKSR"
     gene            9673..10224
                     /locus_tag="GWCH70_3477"
                     /db_xref="GeneID:7969494"
     CDS             9673..10224
                     /locus_tag="GWCH70_3477"
                     /inference="similar to AA sequence:KEGG:GTNG_3462"
                     /note="KEGG: gtn:GTNG_3462 putative DNA binding protein"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding protein"
                     /protein_id="YP_002941929.1"
                     /db_xref="GI:239812982"
                     /db_xref="GeneID:7969494"
                     /translation="MNGNIGEFGALAKEVSLFLDINPNTLRRWSIELEKCGYVFQRDE
                     KDRRIYYKRDILALTEFKKFLEKTQSLENAAKAVVESVKDRENAEKTLSVIVQNSDKI
                     TFTKEELEAYTKRIVEETAQKTAEQILHKFNQTLEQRDRQLVQSLQQTMEQRRLELAA
                     ATEEKQSFWSRLFGKKTKALDKL"
     misc_feature    9709..9948
                     /locus_tag="GWCH70_3477"
                     /note="Helix-Turn-Helix DNA binding domain of
                     transcription regulators from the MerR superfamily;
                     Region: HTH_MerR-SF; cl02600"
                     /db_xref="CDD:243103"
     misc_feature    order(9709..9711,9751..9753,9811..9816)
                     /locus_tag="GWCH70_3477"
                     /note="DNA binding residues [nucleotide binding]"
                     /db_xref="CDD:133389"
ORIGIN      
        1 gtcagggtgt tttttccctg gcgggttcgt ccttcggggt gccagggggc aggggttccc
       61 aatgcaaaga ggaaattgga ataccggaca agggggaaaa gaggatattt cggaaggagg
      121 agacgggacc aatacaacac aatctaattg tgttgtgtta cgtattcgcg gaaaaacgtt
      181 gtttttacag cgtttttcat tttttttaaa ataaaaaaag gttaatgcaa cattttatag
      241 aatagagtga tagaatcagg atggaggtgt taggtttgaa tagaacacaa ccaataaaag
      301 atttggctgt cattgaaaaa ctagggagat acctaaaaaa gacaaatacg cgtgattata
      361 tgttgttccg ttgtatgttg tattttgcat tgcggacaca ggatatatta tcacttaaag
      421 taaaagatgt tcgggggaaa taccaaattt actttaagga acaaaaaaca gggaaaacaa
      481 aaatcatcga aattcaccca gatttgcggc cggaattgga ggaatacacg caaaacatgg
      541 cggatgatga ttggttgttt ccttctcgca tcaaagggcg tccgttgtcg cgagaacaag
      601 catttagacg gttacaggct gcaggaaaaa aatgtgggct tccgtctttg tctgggcacg
      661 ttttgagaaa aaccttttgc tatcatgcct ataaaaatgg cgttccattg catatcctgt
      721 cggaattact aaaccactcg tctgaggcgg ttacgcgccg ttatattggt ttgcaacaag
      781 aagatattaa aagggcttat ttttctgttc aattcaactg ttaaaactct agtaatcttt
      841 ttataggggg tgaaaaagtg gcactacaag aatataaata cagtcaaaat ccgtgcgaat
      901 ttcgctttga aatggatttg tatacctatc tcaaaaagtt atataaaaca aaattaaaaa
      961 acgcttattt tttcatttgc gaaaatgaaa ataaaaattg ggtgcaaaaa acttataaat
     1021 ctacagaaat cgacaaagct tttgcggata tagtacatag caatgcaata gatgtgtatg
     1081 gctctacttg cttacatagt tctgccaaac gtgtaactga aaatgtaatt cagattcctg
     1141 cacttctggt tgacttggat tactataaaa caccttatcg cgatttaact ccaaaagaat
     1201 tagtaaaaaa actagaagaa gtgtatttta aaccgcaaaa aataccttat cccaatgcca
     1261 ttattagttc tgggaatggc gtttatttga tttggttttt aaaatttacg cctggaacag
     1321 acggaaccct gttaaaacga agagtgattg taaaaatact ttatgaaatg ctgaaagaat
     1381 ttggggcgga tgcgaaagcg ttggatgcag cgcatgtgtt tcggttgccg aatacataca
     1441 atagcaaggt tactgataga cacaaacgcg aggtaaaagc gtttgttaat gagctagatg
     1501 actatactct ttcgaagttg tctaaaaagc tgccgagttt gtgggaggta tggaagaaaa
     1561 aacatggtat cagaacggaa caagaaaaaa ggcggaaagg aaaaatcatt tccgttccgc
     1621 aattcaaaac tcgaacacta gcttatgacc atattaaaag tatactgcaa ttaatcgagc
     1681 tacgtaaagg agaatgtaca gggtatcggg aaatgatgtt gttctttttg cgtgacagat
     1741 accatacgat gcacgcaaaa cgtttttatc agcaggatga aacattgttc gcagaaagct
     1801 acgccttcat aaaagaagtt aatcaaaaat ttacaaaacc cttgtcggat aaagaaattc
     1861 gcacaaatac gctcaataaa acaaaactgt ataaatttaa aaccaaaacg attatcgagt
     1921 ggtttgatat tacgctggaa gaacaaattc aactcaaagt gaaaacaaga gaagcgaaaa
     1981 atgagaaatc caaacggcaa atgagagaac tgagaagaaa acgtggagct ttaacacgac
     2041 gtgaatatct taaacaacag cattctataa cagatgataa attgtttaaa ttaaaagagt
     2101 tgctagaacg ttatccaaac gccaaacgca aagaattagc agaaatgctt ggcgtttcag
     2161 tttatagagt agaccaactc aaaagggaat taaaaagttt gtagtggttc gttacttatg
     2221 tattattaat aaataatatg acttgtgtcc ctgtggcatt agctctgcta atgccttttt
     2281 tattttgaat taaaaagttt gtagtggttc gttacttatg tattattaat aaataatatg
     2341 acttgtgtcc ctgtggcatt agctctgcta atgccttttt tattttgaat taaaaagttt
     2401 gtagtggttc gttacttatg tattattaat aaataatatg acttgtgtcc ctgtggcatt
     2461 agctctgcta atgccttttt tattttgaat taaaaagttt gtagtggttc gttacttatg
     2521 tattattaat aaataatatg acttgtgtcc ctgtggcatt agctctgcta atgccttttt
     2581 tattttgaat taaaaagttt gtagtggttc gttacttatg tattattaat aaataatatg
     2641 acttgtgtcc ctgtggcatt agctctgcta atgccttttt tattttgaat taaaaagttt
     2701 gtagtggttc gttacttatg tattattaat aaataatatg acttgtgtcc ctgtggcatt
     2761 agctctgcta atgcctccgt atttcttttt tatttgttga tttgtctgtg aggggattgg
     2821 ttctatccat tccctctctc tttttgtaaa aaccttccca gtggacctgg tgtaagattc
     2881 atgctatatt tagtgttagt gaggtgttat tatggctaat gcgatatcgt tttttgcagg
     2941 agctggcggt ttggatttag gaataaaaag agctggcttt aatatattat tgagtgttga
     3001 aatagaggaa acttactgtc agacattgcg ccaaaatttt gaaggcttga atgtggtttg
     3061 tggagatatt atggaatata gtcgtgaacg tgtttatcaa gaagcgggat tagcagaaca
     3121 tgaagaagtg gatttgatga taggaggaag tccttgccag tctttcagta cagcaggaaa
     3181 gaggcaggca tttgcagacc ctaggggaca agcaatgtta aagtatgcgg atttggttaa
     3241 tgaggttcga ccacgtttct ttttattgga gaatgtgaaa ggattgcttt cggctgcttt
     3301 aaaacatcgt ccactgaatc aaagagggcc ggggcatccg cctttgacgg aggaggagca
     3361 accgggtagt gctttagaat acttattgga acggtttggg gattatgatg taaaaatcaa
     3421 attgattaat gcagctgatt atggcgttcc tcaaaaaagg gaacgtgtct tcattattgg
     3481 tgttcgtaat gatttaaata ttcaatatga atttccagaa ccaacgcata atgaagatgg
     3541 ttcggatgga aaacttcctt gggtgacagt aggggaggtt ttggagaact tacaggtgga
     3601 agagcatcat tttatacctt actcaaaaga acgactccgt ttcatgaaac tgattcccaa
     3661 aggtgggggc aattggagag atttaagagc atatggggaa gaagtggttc gagaagcgat
     3721 gggcggtgct tactattctg ggggtggaaa agtaggtttc ttccgtcgta ttgacagtaa
     3781 taagccatct cctactcttt tgacatcacc agcacaaaag agtacaaatt tagggcatcc
     3841 atttgaggat agaccgttaa gtattgaaga atatttagcc attcagggat ttcctcctgg
     3901 ttttcgggtg gctggaacgt taatgcaaca atatacccaa ataggaaatg cggttccggt
     3961 tcctcttgct gaaatactag gagcatccat tttagagcga ttacgcaccc ttcaaacggt
     4021 ataagactta ttgtaaaaaa ctagattctc tcgtaaactg ggatgtagag aatctagttt
     4081 ttttattgag ggagttgttg ggggttggat gaaaagacaa aagaaagagt tcgccagcat
     4141 gtaaaggctg tcatggagcg attgattgca aaaaggactg tggaagagcc gttctctgcg
     4201 aaagaaattg aagaaaaaaa tccatttggt tatctattgg ttcccatcga agtgtggaaa
     4261 gggtctaaaa tggagcgttc gtttgtaacg acattaggac agggaatctt tgaacagtta
     4321 ggaaaaatta ttgcggaggg aacgggagct tttgctgcca atcagtacga taaaacgttg
     4381 acgattaata catttcgtac tgaaaagatt gattcgatta ttaagaaaca gcgttcgaaa
     4441 ccgaaaaaag gcgagaaaag agtgcctcct gatttatcaa aagaattaga cgatattgct
     4501 aggctggaga atgagaagta tgaaattata actgtaaaat ctgatttata catccgcaga
     4561 ccagatggaa aagaagaata ttattctctt aaaacggtca agccgaacct agaccaaacg
     4621 gcggaagcta agaaaaatct attgctttta agaactgggg accctaattg tgaggcatat
     4681 tttgctttac catataatcc tgctggtgaa ggaaatcctt attccaaagc aggacactcc
     4741 attcctaata ggctgtttaa tatggatgat gagcgatttg tattaattgg ctcggcgtta
     4801 tggaataaaa tcggaaacga tgaaaataca tatgaagagc tgctagagat tttccgagaa
     4861 gttgggaaag agtctgctga aagaatacga aaagaatatt ttggattata gaaaaagcgt
     4921 tttttaacat aaattagcca gaagtccccc acctctacag gtgggggatg aatggctttt
     4981 gttgatgcga aaaacatttt ttgcattctt ctcttgcttt tggatttggg acggagtaca
     5041 ctaaaaataa aaaagcaaac atgacctgtt tgcaagaaga agaatgattc ttcttctaga
     5101 aatgaggttt ttacgtatcg acggactgcg taagcaggaa gtcgtagatg gcgagttccc
     5161 gcccttccgc atccaagatg cggatgttgc ctgtcgttcc caacgggacc gatgccaggc
     5221 ttgcctgcag ataggctgcc aaccagccta tcatgcatcc accccgaagg gaaaacagga
     5281 aactcccact tcgataagtg ggagaggttc atttttagtt tagaagacaa tgaatgatag
     5341 attcattgtc tttttttgtt tgttcatcat acgaacagac cgcaatgttc caattcagaa
     5401 cattttattt tttgttcata acttgaacat taaggtgcct aaaagattaa atctcccgtt
     5461 ttggatggga gattttttat tttttgaccg ctagaaatgg gtctattttt tatttttttg
     5521 tgtgggggag tatttttata ctctcttttt ttggaacgaa ttttgacggt tttttttggt
     5581 cctaatgata ggaatattaa aaagtttgta gtggttcgtt acttatgtat tattaataaa
     5641 taatatgact tgtgtccctg tggcattagc tttgctaatg ccttttttat tttgtttgat
     5701 tttctttctc ttggtggggg gacggttctc tccttcattg gaggaggggg atgtccttgg
     5761 tatttttatg ttttttgttc ttgtgggaag aaattgagtt ataattaagg aaacctttct
     5821 aggaggggaa attatgtatt ttgtgaatgc tgagcataag aggaactatg attatctgat
     5881 ggggttgtat cgattggaac caggtcagaa tgtggagtat gaagctagta tttatattgc
     5941 tgcttatcca agtgtttttg cttgttttgg gaaaacgata agcactgaat ttggtcctct
     6001 tgcttatttg agcgatgaag aacttgcgga ggagttcgga cacaattttg gggctttgac
     6061 gggaacgacg cgacgtatgg ttgcttgtgg gatgagtttg tacaacggat tggaagtatc
     6121 tctttcggat gttttttcgt ctttggggga agaagaattt aatgtgttga ttcaagcgat
     6181 taaaattcgt gcaaaaagat aaaaagagct aatctcccat tttggatggg agatttttta
     6241 ttttttgacc gctagaaatg ggtctatttt ttattttttt gtgtggggga gtatttttat
     6301 actctctttt tttggaacga attttgacgg ttttttttgg tcctaatgat aggaatgcaa
     6361 acatagattg atagtcgaaa aaaaatacac ttttttcacc acttttccag aaattggatt
     6421 acgataataa tgggttttgc gaaccctagt caatgaatat gggcggggaa ggtttggaaa
     6481 cgagtgcaac ggcgtttcca gcacttccga aggaagtgag ttgggaaaat gataagcaac
     6541 gtaatttgaa taaaaaaatt ggaaggaggg gttcgtgaaa cgagatatgt tacgtttctg
     6601 ttctgcggaa atttgcttct aaaaatttaa tattgccttt gccgagatgg tggctgccaa
     6661 ccacctgcac gactgccaag tttttaaatt agtttgtgta gggatggctg ccaaccatct
     6721 cctacacaaa tatttaactt atgccctatt ttttgcggct caccatatcg ccttgccaga
     6781 ggtgatagag gtgggtggag tggcgtgaaa acaggaccgc caatcctgga accatagcca
     6841 cttcggagga cgctgcgtgc atcgagaggt gctggcgcgg tgtcaacggg ggctgcgagt
     6901 aacatcgctt ctacccgatg ggttatgcgt atatgggcga cacagaaata gtgattccga
     6961 ccatactcgg aattgctcgt cacattcgaa gtgggtgcaa atcccaccta gggggagttg
     7021 ggaaaccgct caacctagcg cggcggggga ggttgcggca gtaatgcttg caactaaata
     7081 gctcaacccg ttaaggctca caggtggctg tgagtaaagc gccctacccg tttaggcggg
     7141 cgcaaccgcg taagagattg caaagacgcg aggctaaacc gcgtagaagg cgtagcgtag
     7201 ccgaaagtct tgccataagg caagatgagt aaggggatgc ttccatattc ggtcccgtgc
     7261 ctattttggc acacaagcta cggctggagc gacgtcagtg caatctcgtc gaatgtgact
     7321 aatgtggcaa ggaccgaacg gtctgggtgg caccagatgc gaggtctgag agcttaactg
     7381 agtaggttcc ctgtgggggc attagagctg cagggcagtg gctcttgtgt tagcggaatc
     7441 gggtggcgcc gattcaataa cgctagaccg aaaggttaac tcccatgcaa aaaagggcat
     7501 aagcagtacc tttggcaaag gtgctggact tggcagtcgt gcagaaggtt ggcggaaaac
     7561 ctttactttt aaagaaaccg ccatataatc accgggaggt gatggaatat gtgcactaaa
     7621 tatttgtgta aacgttggca agggttacta gaagcaatta ttaatttcgc agggcaggga
     7681 tatgtgtatt atcacgtaac atatctacca gcaaaaaaac taaataaagc agacaaaata
     7741 gaccaaaaaa tactgcaaaa atataatcta ataaacgata caaaagataa acgttaccat
     7801 cgcaaaaaac ggggtctggc aaacttccgt tatttgcgct gggagcatat tattgtcatc
     7861 ctccacacag aaggaacaat tattcaacga ggacaagaag aaaaacatct aacacgtttt
     7921 aaactagaac aagcaaaaaa acatgctgtg acttatgatg attcgtttag tgatattcgt
     7981 gtgaagccaa tggtgcttgc tatttcggag cagctggaac tagaagtgcg ttatttaccc
     8041 aaaaaaaatg tcggacaaaa attaacagtt cgtttaacca aaaaaatgtt tcgcgacaaa
     8101 aaagccgaat tggccgcatt ggcagaaaaa cgcgcgaaag acaaaattgt gtatgtcttt
     8161 aatgccctta ataacctccc tgcgtggcgt ggcattatca ttcaaaaacg gcagttgtat
     8221 cgttttgtga tgacgcaagc cagaaaacac aattttacgt ttcaaaaagg tgaattaatg
     8281 gttggcgatt atcgcccaaa agagaaggac attttcgttt aacataaatt agccagaagt
     8341 cccccacctc tacaggtggg ggatgaatgg cttttgttga tgcgaaaaac attttttgca
     8401 ttcttctctt gcttttggat ttgggacgga gtacactaaa aataaaaaag caaacatgac
     8461 ctgtttgcaa gaagaagaat gattcttctt ctagaaatga ggtttttacg tatcgacgga
     8521 ctgcgtaagc aggaagtcgt agatggcgag ttcccgccct tccgcatcca agatgcggat
     8581 gttgcctgtc gttcccaacg ggaccgatgc caggcttgcc tgcagatagg ctgccaacca
     8641 gcctatcatg catccacccc gaagggaaaa caggaaactc ccacttcgat aagtgggaga
     8701 ggttcatacc aagacaataa agagcaaaaa agcccattat gtgggctttt ttatttgtct
     8761 taaaaggaaa tgaacgcgac acaccttgtc gcgttatatg tgcaattaga ggacacatgc
     8821 gacatgtgac gcggttatgg gaactttttt gattggttta cgaaacgatg tcggcagatg
     8881 cgatatatta gcgttatcgg attttaaaca gtggctaaga aaaacatata gtttggaaaa
     8941 cgcaataaaa acagttgtat caacgtttaa agaaaaagaa aacgcagtaa aaacgctaag
     9001 cgttatcacg caatataacg ttcaaaaaag gagaatttga aaaacacacg aaaaaaatta
     9061 tagaactaat ttccaaaaag gcgatggaag caagggatta gagaggggtg gatttttgtg
     9121 atacgtatgg aagaaatctc aatttcaatt cccaaagagg ttttagaaaa agtcgagaaa
     9181 agtatgggtg gttgggacga ccgtaatcga gtcattacga atcacttggt gtacttttat
     9241 cgtttgttag aaactgaaaa aaggacgctt accaaaatct tttcggaaga agagctgttg
     9301 tgtatttatg ctgcctttaa cgggacaatc gtcgattttc ggatgactcc aggaatgttt
     9361 aaagccactg ttagagatgc tttaaaatac ggcgaattgg attttcttat aagcgaacat
     9421 cgcattgacg cgggtgcttt tctacaaaaa attgatggtt tatccgattt gcaaactttc
     9481 gttctcttgg tggatgccca agaatattgg gcagagaaat ctagataaaa ttaatatggg
     9541 gatagtgtgg tacataaagg tagatggaat aaaaaagccc gcataatggg ctttttttta
     9601 ttttttttat tggataatag gatttatata tgtcacgata gaaaagaata aaataaaggt
     9661 ggtgaacgcg ttgtgaacgg taatataggc gaatttggtg ctttggcaaa agaagtgtcg
     9721 ctgtttttag atattaatcc aaatacgctc cgtagatggt ctattgagtt agaaaaatgc
     9781 ggttatgtgt ttcaacgtga tgaaaaggac cgtcgaattt actataaacg agatattttg
     9841 gcgttaacgg agtttaaaaa gtttttggaa aaaacacaaa gcctagaaaa cgcagcaaaa
     9901 gctgttgtag aaagtgttaa agacagagaa aacgcggaaa aaacgctaag cgttatcgtg
     9961 caaaatagcg ataaaattac gttcacgaaa gaggaactag aagcgtatac gaaacgcatt
    10021 gtagaagaaa cggcacaaaa aacggcagaa cagattttgc ataaatttaa tcaaacgcta
    10081 gagcaacgcg acagacagct tgtccaaagt ttacaacaaa ccatggagca aagacgcctg
    10141 gaactcgctg ctgcaacaga agaaaaacag tctttttggt cccgtctctt tgggaaaaag
    10201 acaaaagcgt tggacaagct ctgaggatat gccgagaaaa ctctttgcgt tggggtaagc
    10261 cccctgggta tgctagaagg cggaccc
//
