LOCUS       NC_022587               4094 bp    DNA     circular CON 28-OCT-2013
DEFINITION  Lactococcus lactis subsp. lactis KLDS 4.0325 strain Lactococcus
            lactis subsp. lactis strain plasmid 1, complete sequence.
ACCESSION   NC_022587
VERSION     NC_022587.1  GI:554074371
DBLINK      BioProject: PRJNA225028
KEYWORDS    RefSeq.
SOURCE      Lactococcus lactis subsp. lactis KLDS 4.0325
  ORGANISM  Lactococcus lactis subsp. lactis KLDS 4.0325
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
            Lactococcus.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Chun,Y.X.
  TITLE     Phenotypic and genotypic characteristics of lactic acid bacteria
            isolated from sour congee in Inner Mongolia of China
  JOURNAL   J. Gen. Appl. Microbiol. 57 (4), 197-206 (2059)
REFERENCE   2  (bases 1 to 4094)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2013) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 4094)
  AUTHORS   Chun,Y.X.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2013) Northeast Agricultural University, Key
            Laboratory of Dairy Science, Ministry of Education, No.59 Mucai
            Street, Harbin, Heilongjiang 150030, China
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence is identical to CP006767.
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: http://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            Source bacteria/DNA available from Yutang Wang
            (wangyt@neau.edu.cn).
            
            ##Assembly-Data-START##
            Assembly Method       :: SOAPdenovo v. 1.05
            Coverage              :: 1800-folds
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 09/24/2013 15:29:34
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS+
            Annotation Software revision :: 2.1 (rev. 411575)
            Features Annotated           :: Gene; CDS; rRNA; tRNA;
                                            repeat_region
            Genes                        :: 2,732
            CDS                          :: 2,593
            Pseudo Genes                 :: 56
            rRNAs                        :: 19 ( 5S, 16S, 23S )
            tRNAs                        :: 64
            Frameshifted Genes           :: 40
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Lactococcus lactis subsp. lactis KLDS 4.0325"
                     /mol_type="genomic DNA"
                     /strain="KLDS 4.0325"
                     /isolation_source="home-made koumiss"
                     /sub_species="lactis"
                     /db_xref="taxon:1399116"
                     /plasmid="1"
                     /country="China: Sinkiang"
     gene            complement(205..297)
                     /locus_tag="P620_13655"
                     /db_xref="GeneID:17327160"
     CDS             complement(205..297)
                     /locus_tag="P620_13655"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="REF_PRJNA218564:P620_13655"
                     /protein_id="YP_008691090.1"
                     /db_xref="GI:554074372"
                     /db_xref="GeneID:17327160"
                     /translation="MKTILRFVAGYDIASHKKKTGGYPWERGKA"
     gene            298..2019
                     /locus_tag="P620_13660"
                     /db_xref="GeneID:17327155"
     CDS             298..2019
                     /locus_tag="P620_13660"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="REF_PRJNA218564:P620_13660"
                     /protein_id="YP_008691091.1"
                     /db_xref="GI:554074373"
                     /db_xref="GeneID:17327155"
                     /translation="MKFLKDKSIFDKSIKFKMVLPIFLGIIGVLFSLLVPLNFKKIID
                     SISQGINIDFKVFLIIFVLILLQLFFQIFSDYWLQKIGISTVGEIRLKIISKVLSLRK
                     SYFDSNLSGDVSSILVNDASALSYLLSTTIPSIINSVISVVLITLMLFYLSLNLSIVI
                     IVLFPIMFLIYLPIGRILSKVSLEYQDELGKFNHYSQFITTENNFIKTAATEKLEYKK
                     GQKNVLNLMSIGIRQAKVFAITTPLFYTVTIFGALLTITYGIYLVQHNFLTLGSFVAY
                     ITLFIQILNPLSAIGDSFSHIRGIDGATKRIFSILNEDEEDIMLGNSNLEFSELNFKS
                     VDFTYNTGEKNILNDVTFKCDVGKTISIVGPSGSGKTTIFSLIEQFYTIDGGKIYIDG
                     QDISSISLNHLRSNIGYVSQQYPLILGTIRDNLVYGIKNKVISDNELIRVSKLTNFFE
                     VIDKLPEGLNTFVGDKGVLLSEGQKQRLSVTRVLLLNPKILLLDEITSALDSYSEKVI
                     QNTLEGFGKDKITIMIAHRLSTVKNSDFIIFLENGKITGIGNHEELYSKHDLYKSFVQ
                     NQLTKNM"
     gene            2230..2625
                     /locus_tag="P620_13665"
                     /db_xref="GeneID:17327156"
     CDS             2230..2625
                     /locus_tag="P620_13665"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="REF_PRJNA218564:P620_13665"
                     /protein_id="YP_008691092.1"
                     /db_xref="GI:554074374"
                     /db_xref="GeneID:17327156"
                     /translation="MTKTKMKMTEITPLEAVKRLAELKALDIPDTLKVVDAESGEFEN
                     DKNGLIRFGKLICADSEAYEALKAVGKEELLTLQTVKIKQFDGTDLSPYVGKTLKTAG
                     FDFEFQKKNRFGDLEGVCFKVALDEIELA"
     gene            2626..3141
                     /locus_tag="P620_13670"
                     /db_xref="GeneID:17327157"
     CDS             2626..3141
                     /locus_tag="P620_13670"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="REF_PRJNA218564:P620_13670"
                     /protein_id="YP_008691093.1"
                     /db_xref="GI:554074375"
                     /db_xref="GeneID:17327157"
                     /translation="MIGYYELSKKVGKSVSTLTEWKRQIEQLTDYQFQQEKKVAGKFK
                     GSRRARPHKIVISAVAFSEEEVSLFISLKDKIADKGRKRAICEIFGNNLAEMDEISRL
                     KVVIQEWKSDYADLREDYNQNVKEFNQLLKSKKLADERYSECFKQKEELLQELEELKS
                     RKLGARLFNKK"
     gene            3142..3237
                     /locus_tag="P620_13675"
                     /db_xref="GeneID:17327158"
     CDS             3142..3237
                     /locus_tag="P620_13675"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="REF_PRJNA218564:P620_13675"
                     /protein_id="YP_008691094.1"
                     /db_xref="GI:554074376"
                     /db_xref="GeneID:17327158"
                     /translation="MKKNQVGSILKDKNSEGKTEIGNNVKNKIWN"
     gene            3228..4094
                     /locus_tag="P620_13680"
                     /db_xref="GeneID:17327159"
     CDS             3228..4094
                     /locus_tag="P620_13680"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="REF_PRJNA218564:P620_13680"
                     /protein_id="YP_008691095.1"
                     /db_xref="GI:554074377"
                     /db_xref="GeneID:17327159"
                     /translation="MELAKALMELGYLNFSNVYQCAEVLKFQQIETGQLKLKQTWFCK
                     NKLCPICNWRRSMKYAWQTEQIVNEAIIREPKGRFLFLTLTIKNVEGQDLEETMKGLT
                     KAFDRLFRRAKVKKNLLGYLRSLEITLNEETGLYHPHLHILLFVTSTYFKNSNSYIPQ
                     KEWSEMWQQSAKLDYLPIVDVRAVKSKKNQLNQEIGLKKAILETAKYPTKPINFDREN
                     LEVIDNLAKALYRKRQIGYGGIFKDIRKELFLDDVENGDLVHASEDEKGNSEGQEIVA
                     IWNWERLNYFIK"
CONTIG      join(CP006767.1:1..4094)
ORIGIN      
        1 atgataattg taggaattat atttttgttc gtatttttag ctataacaat aggtttatct
       61 tttgaactgt ttaagaaaaa gaaaagaaat attggaacct ttgtgttttt tgctatagca
      121 ttattttgta ctgtcatgtt tgcctatttg ttattgacta atcaatatgt tccaattgat
      181 tataatgtac caagatatgc ataattaagc ttttccacgt tcccatggat agccgccagt
      241 tttcttttta tgactagcaa tatcgtagcc agcaacaaaa cgtaggattg ttttcatatg
      301 aaatttttga aagacaaatc tatttttgat aaatcaatta aatttaaaat ggtattacct
      361 atttttttag gaattattgg agtactattt tccctattag ttccgttaaa ttttaagaaa
      421 attattgatt caattagtca aggtatcaat atagatttta aagttttctt aattattttt
      481 gttttaatat tactacaatt attttttcaa attttttcag attattggtt acaaaaaatt
      541 ggaatatcaa ctgtaggtga aattagatta aaaataattt caaaagttct ttctttaaga
      601 aaaagttatt ttgattcaaa tttgtctgga gacgtttcta gtattttagt caatgatgct
      661 tcagcattat cttatttatt aagtacaact attccatcta taataaattc agtaatttca
      721 gtcgttctga taactttaat gcttttttat ttaagtttaa atttatcaat cgtcatcatt
      781 gttctttttc caataatgtt tttaatctac ttaccaatag gaagaatttt atctaaagtt
      841 tctcttgagt atcaggatga attgggaaaa tttaatcatt attctcaatt tataacaacc
      901 gaaaataatt ttataaagac agctgcaact gaaaagctag aatataaaaa aggacaaaag
      961 aatgtactta atttaatgag cataggtata agacaagcta aagtttttgc tattaccact
     1021 ccattattct atactgttac tatttttgga gcattgctaa ctattacgta tggaatttat
     1081 ttagttcaac ataatttttt gaccttagga agttttgtag cgtatattac actatttata
     1141 caaatattga accctttaag tgctattggt gactcatttt cccacataag aggaattgat
     1201 ggtgctacca aaaggatatt tagtatttta aacgaagatg aagaggatat aatgttagga
     1261 aattcgaact tggaattttc tgagttgaat tttaaatctg ttgattttac atataacaca
     1321 ggagaaaaga atattttaaa tgatgtgact ttcaagtgtg atgtaggaaa aacaatttca
     1381 attgtcggac cgagtggttc tggcaaaaca actatatttt ctttgataga acaattttat
     1441 actattgatg gtggtaaaat ttatattgat ggtcaagata tctccagtat ttctctaaat
     1501 cacttgagat ctaatatagg atatgtttca cagcaatatc ctcttatttt gggaacaata
     1561 agagataatt tggtttatgg tataaaaaat aaggttatct cagataatga attgattaga
     1621 gttagtaaac taactaattt ttttgaagtt atagataaat taccggaggg attaaacact
     1681 tttgttggtg ataaaggggt actattatct gaaggtcaaa aacaaagatt atctgttact
     1741 agggtattat tattaaaccc caaaatttta ttattagatg aaatcacttc agcattagat
     1801 tcttattctg aaaaagtcat tcaaaatact ttggagggtt tcggtaaaga taaaattacg
     1861 ataatgattg cccatagatt atcgacagtc aaaaattcgg attttattat attcttggaa
     1921 aatggtaaaa ttactggaat tggtaatcat gaagagttat actctaaaca tgatttgtat
     1981 aaatcatttg ttcagaatca attaactaaa aacatgtaaa tgaaaaaaca ccccattcat
     2041 agagaattag ggtgttcttg tatttacagt tttatttatc tactgcacaa caaaaatgct
     2101 ctaatctcta tatttttttt aaatataaaa atagaaacat ttccgttgca acgcaatagt
     2161 catcatcatc ttgctttggt atggaaattc tccaaaattg gagttctata tttttttatt
     2221 ataacataaa tgacaaaaac aaaaatgaag atgacagaaa ttaccccact cgaagccgta
     2281 aaacgattgg cagaattgaa agcattagat attcctgaca cgttgaaagt ggttgatgca
     2341 gaaagtggag aatttgaaaa tgataaaaac gggttgattc ggtttggtaa attgatttgt
     2401 gctgatagtg aagcatatga agccttgaaa gcagtaggta aagaagaatt actgacactg
     2461 cagacggtaa aaattaagca atttgatggg acagatttgt caccttatgt tgggaaaaca
     2521 ctcaagactg caggttttga ttttgaattc caaaagaaaa atcgtttcgg tgatttagaa
     2581 ggggtctgct ttaaagtagc ccttgatgaa attgaattgg catgaatgat tggttattat
     2641 gaactctcga aaaaagtagg aaaatcggtt tctaccttga ccgaatggaa aagacaaatt
     2701 gagcaattaa ctgactatca attccaacaa gaaaagaaag tggcgggaaa atttaaaggt
     2761 tcacgtcgag caagacctca caaaatcgtt ataagcgccg tagcattctc tgaagaagaa
     2821 gttagtctat ttatatcact taaagataaa atcgctgata agggtcgaaa aagggcgata
     2881 tgcgaaatct ttggaaataa tcttgctgaa atggacgaaa ttagtcgatt aaaagttgtg
     2941 attcaggaat ggaaatcaga ttatgctgat ttaagggagg attataatca aaacgtgaaa
     3001 gagtttaacc aactgcttaa atcaaaaaag cttgctgatg aacgttattc tgaatgtttt
     3061 aagcaaaaag aggaattact ccaggagctg gaagaattga aaagtcgaaa attgggtgct
     3121 cgattgttta ataaaaaatg aatgaaaaaa aatcaagtag ggtctattct caaagataaa
     3181 aatagtgagg gaaagaccga aattggcaac aacgtaaaaa acaaaatatg gaattagcaa
     3241 aagcacttat ggagttaggc tatcttaatt tttcaaatgt ttatcaatgt gctgaagttt
     3301 tgaaattcca acaaatagaa accggtcaat taaaattaaa acaaacatgg ttctgcaaaa
     3361 ataaattgtg tccgatttgt aattggaggc ggtcaatgaa atatgcttgg caaactgaac
     3421 aaattgtgaa tgaagcgata attcgtgaac ctaaaggacg ctttcttttc ttaacgttga
     3481 cgattaaaaa tgtcgaagga caagatctag aagaaacaat gaaaggtttg acaaaagctt
     3541 ttgaccgatt gtttcgacgt gcaaaagtga aaaaaaactt gttaggttat cttcgaagtt
     3601 tagaaataac gctcaatgaa gaaacagggt tatatcatcc tcacttacat atacttcttt
     3661 ttgtaacttc aacatatttt aaaaatagta atagttacat tccgcaaaaa gagtggtctg
     3721 agatgtggca acaatctgca aaattggatt atttgccgat tgtggatgtt cgggcagtaa
     3781 aatcgaagaa aaatcaattg aatcaagaaa taggtttaaa aaaggcaatt ttagaaacgg
     3841 cgaaatatcc gacaaagcct attaattttg atcgagaaaa tttggaagtc attgataatc
     3901 tggcaaaagc cctttatcga aaacgacaaa ttggttatgg tggaattttt aaagatattc
     3961 gaaaagaact ctttttagat gacgttgaaa acggagattt agttcacgct tctgaagatg
     4021 aaaaaggaaa ttctgaaggt caagaaattg tggcgatttg gaattgggaa cgactgaatt
     4081 attttattaa ataa
//
