LOCUS       NC_012972               9816 bp    DNA     circular BCT 15-AUG-2011
DEFINITION  Methylovorus glucosetrophus SIP3-4 plasmid pMsip02, complete
            sequence.
ACCESSION   NC_012972
VERSION     NC_012972.1  GI:254028270
DBLINK      Project: 59367
KEYWORDS    .
SOURCE      Methylovorus glucosetrophus SIP3-4
  ORGANISM  Methylovorus glucosetrophus SIP3-4
            Bacteria; Proteobacteria; Betaproteobacteria; Methylophilales;
            Methylophilaceae; Methylovorus.
REFERENCE   1  (bases 1 to 9816)
  AUTHORS   Lapidus,A., Clum,A., Labutti,K., Kaluzhnaya,M.G., Lim,S.,
            Beck,D.A., Glavina Del Rio,T., Nolan,M., Mavromatis,K.,
            Huntemann,M., Lucas,S., Lidstrom,M.E., Ivanova,N. and
            Chistoserdova,L.
  TITLE     Genomes of three methylotrophs from a single niche reveal the
            genetic and metabolic divergence of the methylophilaceae
  JOURNAL   J. Bacteriol. 193 (15), 3757-3764 (2011)
   PUBMED   21622745
REFERENCE   2  (bases 1 to 9816)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2009) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 9816)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H.,
            Bruce,D., Goodwin,L., Pitluck,S., Clum,A., Larimer,F., Land,M.,
            Hauser,L., Kyrpides,N., Mikailova,N., Kayluzhnaya,M. and
            Chistoserdova,L.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUL-2009) US DOE Joint Genome Institute, 2800
            Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CP001676.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 4086190
            Source DNA and bacteria available from Ludmila Chistoserdova
            (milachis@u.washington.edu)
            Contacts: Ludmila Chistoserdova (milachis@u.washington.edu)
                      David Bruce (microbe@cuba.jgi-psf.org)
            Annotation done by JGI-ORNL and JGI-PGF
            Finishing done by JGI-PGF
            Finished microbial genomes have been curated to close all gaps with
            greater than 98% coverage of at least two independent clones. Each
            base pair has a minimum q (quality) value of 30 and the total error
            rate is less than one per 50000.
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. it is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            
            ##Metadata-START##
            Organism Display Name :: Methylovorus sp. SIP3-4
            GOLD Stamp ID         :: Gi03952
            Funding Program       :: DOE-CSP 2009
            Oxygen Requirement    :: Aerobe
            Cell Shape            :: Rod-shaped
            Motility              :: Motile
            Sporulation           :: Nonsporulating
            Temperature Range     :: Mesophile
            Gram Staining         :: gram-
            Biotic Relationship   :: Free living
            Habitat               :: Fresh water
            ##Metadata-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9816
                     /organism="Methylovorus glucosetrophus SIP3-4"
                     /mol_type="genomic DNA"
                     /strain="SIP3-4"
                     /db_xref="taxon:582744"
                     /plasmid="pMsip02"
     gene            103..351
                     /locus_tag="Msip34_2831"
                     /db_xref="GeneID:8174800"
     CDS             103..351
                     /locus_tag="Msip34_2831"
                     /inference="ab initio prediction:Prodigal:1.4"
                     /note="KEGG: asa:ASA_1269 transcriptional protein"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_003056831.1"
                     /db_xref="GI:254028271"
                     /db_xref="InterPro:IPR001387"
                     /db_xref="GeneID:8174800"
                     /translation="MSTGYGKRIRLVREHLGLGRAEFCNETGIPKQSLINYETERTKA
                     NAEVLGAIANKWPEYAAYLLTDKTFVIQKNPEHDQINN"
     misc_feature    118..264
                     /locus_tag="Msip34_2831"
                     /note="Helix-turn-helix XRE-family like proteins.
                     Prokaryotic DNA binding proteins belonging to the
                     xenobiotic response element family of transcriptional
                     regulators; Region: HTH_XRE; cd00093"
                     /db_xref="CDD:28977"
     misc_feature    order(130..132,142..144,217..219)
                     /locus_tag="Msip34_2831"
                     /note="non-specific DNA binding site [nucleotide binding];
                     other site"
                     /db_xref="CDD:28977"
     misc_feature    order(139..141,214..216)
                     /locus_tag="Msip34_2831"
                     /note="salt bridge; other site"
                     /db_xref="CDD:28977"
     misc_feature    order(160..165,196..198,205..207,217..222)
                     /locus_tag="Msip34_2831"
                     /note="sequence-specific DNA binding site [nucleotide
                     binding]; other site"
                     /db_xref="CDD:28977"
     gene            complement(376..1059)
                     /locus_tag="Msip34_2832"
                     /db_xref="GeneID:8174788"
     CDS             complement(376..1059)
                     /locus_tag="Msip34_2832"
                     /inference="ab initio prediction:Prodigal:1.4"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_003056832.1"
                     /db_xref="GI:254028272"
                     /db_xref="GeneID:8174788"
                     /translation="MILFIGVIRMNLYVVVEGVVETIVYPDWISIVNEDLKKVSYLDE
                     VVDNNYILFSAGGYPNVFNVIDSAVEDISESALFDRLVVAMDAESFSYNERYDEIYNH
                     LLQKKVKVDFKIIIQNPCFEAWALGNSGIAPRNPSDEKLKKYKRIFDILRKDPETIPD
                     LPEEQLNKAQFSYEYLKLAIRDKGQHLHYSKNSPKLVCHPKYFSSLKERMEKKNHIRS
                     FNDFITAFI"
     gene            complement(1034..2119)
                     /locus_tag="Msip34_2833"
                     /db_xref="GeneID:8174789"
     CDS             complement(1034..2119)
                     /locus_tag="Msip34_2833"
                     /inference="ab initio prediction:Prodigal:1.4"
                     /note="KEGG: sde:Sde_2755 hypothetical protein"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_003056833.1"
                     /db_xref="GI:254028273"
                     /db_xref="GeneID:8174789"
                     /translation="MILNNYSYKDHDGHGWNFSPVKLRRNNLLVGISGSGKSRWLNSF
                     SNICSYVFNNDQFRAGVWNLEFSAEEKEYAWEFSSNLEQGGSILSEVLKTKSGDVWEV
                     IFSRDENHIYYGGQQLPRLAKNVTGINLLRDEDAIAPVYRAFGKVMRRNFNASDLIEA
                     GAIIQFPSEPESFDFPANISAINPRLYFLKKRNKKMFDYVIDEFKSIFPFIINVDFTS
                     GAELSDNNSFSPLLIFQERNVKESIYLHDLSSGLIKVLLIMTDISTLPEESIYIIDEY
                     ENSLGMNAINFLPDFIRSYSSKIQFITTSHHPYLINNIPIDDWVVFGRNGSNVAVRLG
                     ENLVSRYGASKQDAFVKLINDPFYRGY"
     misc_feature    complement(1157..>1426)
                     /locus_tag="Msip34_2833"
                     /note="Predicted ATPase [General function prediction
                     only]; Region: COG4637"
                     /db_xref="CDD:34256"
     gene            complement(2153..3346)
                     /locus_tag="Msip34_2834"
                     /db_xref="GeneID:8174790"
     CDS             complement(2153..3346)
                     /locus_tag="Msip34_2834"
                     /inference="protein motif:PFAM:PF00589"
                     /note="PFAM: integrase family protein;
                     KEGG: nmu:Nmul_A2184 phage integrase"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="YP_003056834.1"
                     /db_xref="GI:254028274"
                     /db_xref="InterPro:IPR002104"
                     /db_xref="GeneID:8174790"
                     /translation="MALTDLAVKNADPKPKPYKLTDGNGLYLLIKPSGKYWRFDYRFN
                     YKRKTLALGVYPDTTLAHAREKLDKARKLLANDPPVDPGENRKAERASRTAKNESCFE
                     VIAREWVASYMLNKSDLHRQRVFRRLEVFMFPWIGAVSIDQLTAPEILKCIRRIQAQN
                     KIETAHRTLQATGQVFRYAVQTGRAIRDVTHDLRGALPSHNVKHMASFTEPEQVAQLL
                     KAIEGFTGSFTVQTALRLAPLVFVRPSELRKARWADIDLEKKEWRFRVSKTNIDHLVP
                     LSTQAVELLEGIKPVSGHGEWVFMGGHDPKKPMSAAAINAALKRMGYNTQTEITGHGF
                     RAMARTILHERLNFDPYVIEHQLAHKVPDALGAAYNRTKFIEQRKDMMQKWADFLTYL
                     VIRAV"
     misc_feature    complement(2180..3340)
                     /locus_tag="Msip34_2834"
                     /note="integrase; Provisional; Region: PRK09692"
                     /db_xref="CDD:170049"
     misc_feature    complement(2186..3268)
                     /locus_tag="Msip34_2834"
                     /note="Bacteriophage P4 integrase. P4-like integrases are
                     found in temperate bacteriophages, integrative plasmids,
                     pathogenicity and symbiosis islands, and other mobile
                     genetic elements.  They share the same fold in their
                     catalytic domain and the overall...; Region: INT_P4;
                     cd00801"
                     /db_xref="CDD:29502"
     misc_feature    complement(order(2240..2242,2270..2272,2342..2344,
                     2351..2353,2543..2545,2618..2620))
                     /locus_tag="Msip34_2834"
                     /note="active site"
                     /db_xref="CDD:29502"
     misc_feature    complement(order(2240..2242,2270..2272,2342..2344,
                     2351..2353,2543..2545,2618..2620))
                     /locus_tag="Msip34_2834"
                     /note="Int/Topo IB signature motif; other site"
                     /db_xref="CDD:29502"
     gene            complement(3711..4676)
                     /locus_tag="Msip34_2835"
                     /db_xref="GeneID:8174791"
     CDS             complement(3711..4676)
                     /locus_tag="Msip34_2835"
                     /inference="similar to AA sequence:KEGG:Neut_1485"
                     /note="KEGG: net:Neut_1485 SppA protein"
                     /codon_start=1
                     /transl_table=11
                     /product="SppA protein"
                     /protein_id="YP_003056835.1"
                     /db_xref="GI:254028275"
                     /db_xref="GeneID:8174791"
                     /translation="MAVKEIVNSLSEALDVDIHLYSGQISREGYEQLSVILRDEAAHP
                     RAILMLSTVGGDPHAGYRIARALCHHYPEGFSVMIPGLCKSAGTLICLGAKALIMGDR
                     SELGPLDVQVRKKDELFELGSGLDTIQALSYMQSQAMSAFRTYLIELKADAGLSTRMA
                     AEISTKLATGLFSPVYSQVDPVRLGELQRSIEVAYSYGNRLSKKSSNLKSDALNRLVS
                     RYPAHGFVIDRSEARELFENVRSPAGDELDLLNFWYDNPNNDPSGQPMVINLTKLFGD
                     EDDEQQQANVANDGPHEGDGGVDAVEQAIGEDRGNGDPVPIQDAD"
     misc_feature    complement(<4248..>4613)
                     /locus_tag="Msip34_2835"
                     /note="Periplasmic serine proteases (ClpP class)
                     [Posttranslational modification, protein turnover,
                     chaperones / Intracellular trafficking and secretion];
                     Region: SppA; COG0616"
                     /db_xref="CDD:30961"
     gene            complement(4861..6021)
                     /locus_tag="Msip34_2836"
                     /db_xref="GeneID:8174792"
     CDS             complement(4861..6021)
                     /locus_tag="Msip34_2836"
                     /inference="protein motif:PFAM:PF05707"
                     /note="PFAM: Zonular occludens toxin;
                     KEGG: rpi:Rpic_1399 zonular occludens toxin"
                     /codon_start=1
                     /transl_table=11
                     /product="Zonular occludens toxin"
                     /protein_id="YP_003056836.1"
                     /db_xref="GI:254028276"
                     /db_xref="InterPro:IPR008900"
                     /db_xref="GeneID:8174792"
                     /translation="MIHLITGLPGSGKSLYTLSTVKSRADKENRPVFYHGIPELTLDW
                     QQLESADKWVDCPKGAIIVIDECQSTFRPRATGAAVPRHVSQLETHRHDGHDLYLITQ
                     HPMLVDSNLRRLVNYHYHVERFFGFAKSKIHEFHKVRENVDKSTKNSIESHFVYPKEV
                     YTWYKSADMHTVKKRIPMRLMLMVLLPVLFFAIVWYGYRALTGISKSPDLDQLPTVEE
                     SGSAQPNQPAPPQFQKPIYSWGEAQRPRVPDLPFTAPKYDDITKPVMAPRIAAAVLIR
                     GKCTAYTQQGTKIAMDEAVCLQFVENGMFQDFDDGSARFNKDQRDLNQQTMTPNALDD
                     AQRPKGEPVAPAAVAVIPYPTDEESERSPANRVRESQSQNRYPNQPQPYAGS"
     misc_feature    complement(5509..6021)
                     /locus_tag="Msip34_2836"
                     /note="Zonular occludens toxin (Zot); Region: Zot;
                     cl01706"
                     /db_xref="CDD:174664"
     gene            complement(6018..6317)
                     /locus_tag="Msip34_2837"
                     /db_xref="GeneID:8174793"
     CDS             complement(6018..6317)
                     /locus_tag="Msip34_2837"
                     /inference="similar to AA sequence:KEGG:Dtpsy_0843"
                     /note="KEGG: dia:Dtpsy_0843 hypothetical protein"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_003056837.1"
                     /db_xref="GI:254028277"
                     /db_xref="GeneID:8174793"
                     /translation="MPLFISMFWGFAALSLKTLVGRVLVALAITYVTYQGLDVLLSGA
                     RQAALGLLTNVPADVIGAVGLLRLGESINIVFSAIAARYIVQGLTGGVLTRQVIK"
     misc_feature    complement(6054..6293)
                     /locus_tag="Msip34_2837"
                     /note="Protein of unknown function (DUF2523); Region:
                     DUF2523; cl10291"
                     /db_xref="CDD:195959"
     gene            complement(6318..7634)
                     /locus_tag="Msip34_2838"
                     /db_xref="GeneID:8174794"
     CDS             complement(6318..7634)
                     /locus_tag="Msip34_2838"
                     /inference="ab initio prediction:Prodigal:1.4"
                     /note="KEGG: rpi:Rpic_2546 hypothetical protein"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_003056838.1"
                     /db_xref="GI:254028278"
                     /db_xref="GeneID:8174794"
                     /translation="MAHFLRLILLLSMVLSSYAHAANEEIPSIIDYWSKDGMTVTKKP
                     TPEESCQAYTAYSGVSYGSVTKVDNWKYFCNSKNGNIGNITAHEVCPGGVTPTILHTC
                     LVPQCAAGQVRNPTTGTCQESCKSGFVSGVTGSKYFGSDGSTDCLSNCTYSIAVDMCI
                     KLTNGQGACVGQYGNGTGAACSAATNNTALTPEANCMSQGKSFISVGGVTSCVSAGSQ
                     GSQPVTTKNNSSSSSNSSTKDANGNPTGSSSSNSTTNSSATFNGDGTVVVVTTTEKTN
                     EDGTKETKTETKTVSQTSYCAENPTAAQCKAATDSDISGACDAVACKGDAIQCAMAKE
                     QARRNCEWFKENAEWKSKGEALANGTDTTANPAEENNRTIVNLPTALDASSPISATGI
                     QDRTFAVFGRSYTLRLSELNPYLAVVGYVFMALAYVAAGRILAGAV"
     sig_peptide     complement(7569..7634)
                     /locus_tag="Msip34_2838"
                     /note="Signal predicted by SignalP 3.0 HMM (Signal peptide
                     probability 1.000) with cleavage site probability 0.999 at
                     residue 22"
     gene            complement(7748..7954)
                     /locus_tag="Msip34_2839"
                     /db_xref="GeneID:8174795"
     CDS             complement(7748..7954)
                     /locus_tag="Msip34_2839"
                     /inference="ab initio prediction:Prodigal:1.4"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_003056839.1"
                     /db_xref="GI:254028279"
                     /db_xref="InterPro:IPR017441"
                     /db_xref="GeneID:8174795"
                     /translation="MKKVNLYSRKVAAYLAVGMTLAAAQAQAAIDVSAVTEKLGEGET
                     AVAAIGGAILVVWAIKKVYSMIRG"
     sig_peptide     complement(7868..7954)
                     /locus_tag="Msip34_2839"
                     /note="Signal predicted by SignalP 3.0 HMM (Signal peptide
                     probability 1.000) with cleavage site probability 0.946 at
                     residue 29"
     gene            complement(7941..8315)
                     /locus_tag="Msip34_2840"
                     /db_xref="GeneID:8174796"
     CDS             complement(7941..8315)
                     /locus_tag="Msip34_2840"
                     /inference="ab initio prediction:Prodigal:1.4"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_003056840.1"
                     /db_xref="GI:254028280"
                     /db_xref="GeneID:8174796"
                     /translation="MKAFNRIASIHLSRSQVNAMALIMGLLLGGFLTFIWGMTDAYAA
                     ECLTDIGAGQYQIASPQPTEISECVYLIAQPKELTAGAWSLTVEQGQQLAAAIALLWA
                     IGAVFRVLISLLKQKESQHEES"
     sig_peptide     complement(8184..8315)
                     /locus_tag="Msip34_2840"
                     /note="Signal predicted by SignalP 3.0 HMM (Signal peptide
                     probability 0.989) with cleavage site probability 0.831 at
                     residue 44"
     gene            complement(8302..9180)
                     /locus_tag="Msip34_2841"
                     /db_xref="GeneID:8174797"
     CDS             complement(8302..9180)
                     /locus_tag="Msip34_2841"
                     /inference="similar to AA sequence:KEGG:Mfla_1456"
                     /note="KEGG: mfa:Mfla_1456 hypothetical protein"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_003056841.1"
                     /db_xref="GI:254028281"
                     /db_xref="GeneID:8174797"
                     /translation="MGTSLALDQDHVYTELPDYHLTIREFASGMVEMIQRRVNFMDEL
                     RKSRSHGIRGLPRQKTEEEIEASQEENLRRAERRARQKVRFLIQSIGADHLLTLVYRE
                     NMTDSDKLNEDFTRFVRLVREKYPDWLYVGVKEYQERGALHMHVACVGKQDVHHLRKC
                     WYIAIGGNADDAGENTKGQINVRYRKKRFSGQSPIFTCLQLAAYLSKYISKTFAHSRE
                     LGERRYKASRGIPAPKVMRQYLGAFAAIHKEQTFPVATQHTLGIADLMGVFDYQIWAT
                     KDLDILILRGSISESL"
     gene            complement(9250..9516)
                     /locus_tag="Msip34_2842"
                     /db_xref="GeneID:8174798"
     CDS             complement(9250..9516)
                     /locus_tag="Msip34_2842"
                     /inference="ab initio prediction:Prodigal:1.4"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_003056842.1"
                     /db_xref="GI:254028282"
                     /db_xref="GeneID:8174798"
                     /translation="MELKIQVLAISSRTGKSERGEYVRHDLQCFVDQVVGVIPSYDPE
                     FKPEAGFYMASIGWSNYQGRLQPRIEKLTPISQTREHAREQVKA"
     gene            complement(9604..9810)
                     /locus_tag="Msip34_2843"
                     /db_xref="GeneID:8174799"
     CDS             complement(9604..9810)
                     /locus_tag="Msip34_2843"
                     /inference="ab initio prediction:Prodigal:1.4"
                     /note="KEGG: bpy:Bphyt_0267 hypothetical protein"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_003056843.1"
                     /db_xref="GI:254028283"
                     /db_xref="GeneID:8174799"
                     /translation="MDTHFDASQLTVVPLMSRTKFAQHVGVTEDTVTGWINKGYLPVV
                     EIGKYRLVNLALVTKNALEQSFYE"
ORIGIN      
        1 ttaggctcct ttagagttca ttacaaatca taaatataac cagatcagac taaatcttaa
       61 tttggataca ttagggctga ctgtataacg aactggatac acgtgtcaac aggctatgga
      121 aaaagaattc gtttggtgag agaacaccta ggactaggca gggcagagtt ttgcaatgaa
      181 acaggtatcc caaagcaaag cttaattaac tacgaaacag agaggactaa agcgaacgct
      241 gaagtattag gggccattgc caataaatgg cctgaatatg ctgcatactt actgacagat
      301 aagactttcg tgatacaaaa gaatcctgaa catgaccaaa taaacaacta gcctcaggat
      361 taaaagggcc tgtatttaaa taaatgctgt aatgaaatca ttaaaacttc ttatgtgatt
      421 tttcttttcc attctctctt taagagaaga gaaatatttt ggatgacata ctaatttagg
      481 agagttcttt gagtaatgca gatgctgacc tttatcacgt atagccagct ttaagtattc
      541 atatgagaac tgagccttat ttaattgctc ctcaggcaaa tctggaatcg tctcaggatc
      601 tttccttaaa atatcgaata ttcttttata ctttttcaat ttctcatctg acggatttct
      661 tggtgctatt ccagaattgc ctaaagccca agcttcaaaa catggatttt gtataattat
      721 tttaaaatcc acttttactt ttttctgaag taaatggtta tatatctcat cgtacctctc
      781 gttataacta aaactttctg catccatcgc aactacgagg cgatcaaata aagcggattc
      841 agatatatcc tctactgcac tatcaataac attgaagaca ttggggtaac ctccagcaga
      901 aaatagaatg taattattat caaccacctc atccaaataa cttactttct tgagatcctc
      961 attaacgatt gatatccaat caggatacac aatagtttca accactcctt caactacaac
     1021 atataaattc attctaataa ccccgataaa aaggatcatt aatcaacttc acaaatgcgt
     1081 cttgttttga tgcaccatat ctagatacta gattctcacc caaccgaaca gctacatttg
     1141 atccatttcg accaaacaca acccaatcat caattggaat gttattaatc aagtatggat
     1201 gatgagaagt tgttataaat tgaattttac tagaatacga cctgatgaaa tcaggaagaa
     1261 aattaattgc attcattcct aaagaatttt cgtactcatc aataatataa atactttctt
     1321 ctggcaaagt agaaatatcc gtcattatta ataaaacttt tattaaacca gaagataaat
     1381 catgaagata aattgactct ttaacatttc tttcttgaaa tattaggaga ggagaaaaac
     1441 tattgttatc acttagctcc gctccgcttg taaaatcaac attaataatg aatggaaata
     1501 tagacttaaa ctcatctata acataatcaa acatcttctt gttgcgtttc tttagaaagt
     1561 agagccttgg attaatagca gagatgttgg caggaaagtc gaaagattca ggctcactcg
     1621 gaaactgaat aatggctcct gcctctataa gatctgaggc attaaaattt cgcctcataa
     1681 ccttaccaaa tgctctataa actggagcaa tcgcatcctc atctctgaga agattaatcc
     1741 ctgtcacatt ctttgccaac cggggaagct gctgtcctcc atagtaaata tgattttcat
     1801 cgcgagaaaa aatcacctcc caaacatcac ccgattttgt ttttagaact tctgagagaa
     1861 tgctcccacc ttgttcaaga tttgagctaa attcccaggc atactctttt tcttcggctg
     1921 aaaattcaag attccagaca cctgctctaa attgatcgtt attaaaaaca taactgcaaa
     1981 tatttgaaaa tgaatttagc catctggatt tccctgatcc acttataccc actagaagat
     2041 tatttcgtct tagtttgaca ggggaaaagt tccaaccatg tccatcgtga tctttataag
     2101 aatagttatt taatatcata tcttgctccc gaattgggtt cccatgcgaa tatcatacag
     2161 cccgtataac taaatatgta aggaaatcag cccatttttg catcatatct tttctctgct
     2221 cgataaattt agtgcgattg taggctgcac cgagtgcatc ggggactttg tgggctaact
     2281 gatgctcgat aacgtaggga tcgaagttta agcgctcgtg caatattgtt cgggccatgg
     2341 cgcggaaacc gtggcctgtt atctctgttt gggtgttgta gcccattctc tttaaggcag
     2401 cattgattgc cgctgcactc atgggctttt ttggatcgtg tccacccata aatacccatt
     2461 ccccatgccc tgatacaggc ttgatccctt ccagcagttc aaccgcctga gttgagagcg
     2521 gcaccaggtg atcgatgttt gttttgctca ccctaaaccg ccactccttc ttttcaagat
     2581 caatatctgc ccatcttgcc ttgcgtagct cgctagggcg cacgaatacg agtggggcta
     2641 acctcagggc tgtctgtacc gtgaaagagc ctgtgaagcc ttctattgcc ttcagcagct
     2701 gggcaacttg ctcgggttcg gtgaatgagg ccatatgttt gacgttatgc gatggcaacg
     2761 cccctctcaa atcatgcgta acgtcacgta ttgcccgccc tgtttgaact gcatatctga
     2821 aaacctgacc tgttgcctgc aatgtgcggt gcgcggtctc tattttgttt tgcgcctgaa
     2881 ttcgccggat gcatttcaga atctcagggg cagttaactg atcaatagaa actgctccga
     2941 tccaaggaaa catgaagact tccaaacggc gaaacacacg ctggcggtgt aagtcgcttt
     3001 tgttaagcat ataactggcg acccactccc gcgctatgac ctcaaagcag ctttcatttt
     3061 tggcggttcg tgaggcgcgc tccgctttcc ggttctcacc tggatcaacg ggcggatcat
     3121 tggcgagtag cttacgggct ttatcaagtt tttctcgggc atgggctagt gttgtatctg
     3181 gatacacgcc aagagcgagg gttttgcgtt tgtagttaaa ccgataatcg aagcgccagt
     3241 atttgccgga tggcttaata agcagatata gaccattgcc atccgtcagc ttgtagggct
     3301 tgggtttggg atcagcgttc tttactgcga ggtctgtaag ggccatgacg gtatctggta
     3361 atgacggtac ttgagatacc gcgaaagata ccgtcagtga ttatggactg tcaatgaacc
     3421 acaatagaaa tctataaacg aggaaaattt ataactacct gtatttattg atatttaaaa
     3481 atcccatcag acgtcattgg atgactatgg attgcattgt gcggggaatg gtgccggaga
     3541 tagctacaaa acaaatgggc ctaacccagt attgacaagg gtaaggccca tttatgaaaa
     3601 aagataccga cggatatacc gtcaaaatta aagattcaga ttaacatctc gacccaagca
     3661 cacttttcaa ggtggcatta ttggtcttgt ggtttacatc aaaattcgac tcagtctgca
     3721 tcttgtattg ggactggatc accatttccg cgatcttcgc cgattgcctg ctcgactgca
     3781 tcaacgcccc catcaccttc atgtggtccg tcatttgcga cgttagcttg ctgctgctca
     3841 tcatcctcat ccccaaaaag tttagttaaa ttaataacca taggttgccc agaagggtca
     3901 ttattaggat tatcatacca aaaattcaat aaatctagct catcaccagc aggactacga
     3961 acattttcaa aaagctctct agcctcacta cgatcaataa caaacccatg cgctgggtaa
     4021 cggcttacca gcctgtttaa ggcatcactc tttaagttag aggatttttt tgacaatcgg
     4081 tttccatatg aataagcaac ttcgattgac cgttgcaatt cgcctaacct aacgggatcg
     4141 acttgactgt atacaggaga aaacaaccca gtagctagct tagttgaaat ctcggcagcc
     4201 attctagtac tcaagcctgc atctgctttc aattcaatta aataggttct gaaagcactc
     4261 atggcctgcg attgcatgta acttagcgcc tggatagtgt ccagtccaga gcctaactca
     4321 aagagttcgt cttttttacg cacttgaaca tctaaggggc ctagctcact tctatccccc
     4381 ataatgagtg cctttgcgcc taaacatatc aaagtaccgg cacttttgca caatcccgga
     4441 atcattactg agaacccttc cgggtaatga tggcataacg ccctagcaat tctatatcct
     4501 gcatgaggat cgccgccaac agtggataac atcaaaatcg cacgcgggtg agctgcctcg
     4561 tctcttaaga taacagacag ctgttcataa ccttcccgag aaatttggcc cgaataaaga
     4621 tgtatatcga catccagtgc ttctgaaagt gaattaacaa tttctttcac agccattttc
     4681 atctccctaa gactttattt ttattgttaa taatcttaca tccaaacatc ttggttgtat
     4741 tgtgtccgta atataaattt aggcggcgct tcgcgccggg ctacgctccc ggcaccaagc
     4801 accgccttaa cccccataaa aaaagcgcct catgggcgct tttttcttct cctgactcta
     4861 tcaacttccc gcgtaaggtt gcggctggtt tgggtagcgg ttttgagact gcgactcacg
     4921 cactcgattc gccgggctac gctctgattc ctcatccgtg ggataaggaa tcacggctac
     4981 cgccgcggga gctaccggct cacccttcgg gcgctgcgcg tcgtccagtg cgttcggagt
     5041 catggtctgt tggttgagat cgcgttggtc tttattaaac cgagcgctgc catcgtcgaa
     5101 atcctgaaac atcccgtttt cgacaaactg gaggcacact gcctcatcca tcgcgatctt
     5161 ggtgccctgt tgggtgtatg ccgtgcattt accccgaata agcacagcag cggcgattct
     5221 gggggccatg acgggcttgg tgatgtcgtc atacttggga gccgtaaacg gcaggtctgg
     5281 gacgcgtggg cgttgcgctt caccccatga atagatcggc ttttggaatt gcggcggggc
     5341 tggctgattg ggttgagcag atccactttc ttccaccgtt ggcagttggt cgagatccgg
     5401 cgattttgag atcccggtta gagcacgata gccataccag acgatggcaa agaacagcac
     5461 gggcaacagg accataagca tcaagcgcat cgggatacgc tttttaaccg tatgcatgtc
     5521 cgccgactta taccaggtgt agacctcttt cggatataca aagtggctct caatgctgtt
     5581 cttggtggat ttatcgacgt tctcccgcac cttgtggaat tcgtggattt tggacttggc
     5641 aaagccaaaa aaccgctcga cgtggtaatg gtagttcacg agccggcgca gattggaatc
     5701 aaccagcatg gggtgctgcg taatcagata gagatcatgg ccatcatgcc ggtgcgtttc
     5761 caattggcta acatggcgag gcaccgcagc accggtggca cgaggtctaa aggtggactg
     5821 acattcatca atcacgataa tcgccccctt ggggcaatct acccatttat cagcagattc
     5881 gagctgttgc caatctaggg tgagttccgg gatgccgtga tagaacaccg ggcgattttc
     5941 tttatccgcc ctactcttca ccgtggacaa ggtatagagg cttttacccg agcctgggag
     6001 gcctgttatc aggtgaatca tttgatcacc tgccttgtga ggacgccacc cgtcagacct
     6061 tgcacgatgt atcgcgccgc tatggcacta aatacgatat tgatcgattc gcccaggcgc
     6121 aggaggccca ctgccccaat gacatccgcc gggacattgg ttaacagacc aagggcggct
     6181 tgtcttgcgc cagacagcag cacatccagc ccttgatagg tcacataggt aattgccaag
     6241 gccaccagta cacgcccgac caaggtttta agcgacaggg cagcaaagcc ccagaacatg
     6301 gaaatgaata atggcattta taccgctccc gctaagattc ggcctgcagc gacataagcc
     6361 aaggccatga agacataccc gaccactgcc agatatggat tgagttcaga tagacgcagg
     6421 gtgtaactgc gaccaaatac agcgaaggtc cggtcttgaa tcccggtggc ggagatcggg
     6481 ctggaggcat ccaaggcagt gggcaagtta acaatggtcc ggttgttctc ttctgccgga
     6541 ttggcggtcg tatcagtgcc attggctaag gcttcgccct tgcttttcca ttcggcgttt
     6601 tctttaaacc actcgcagtt acgtcgggcc tgttcttttg ccatggcgca ctggatcgca
     6661 tcgcccttgc acgccaccgc atcgcatgcc ccggaaatat cggaatccgt cgccgccttg
     6721 cactgcgcag ccgtgggatt ctcagcgcaa tagctggtct ggctgaccgt tttcgtttcg
     6781 gtcttcgttt ccttggtgcc gtcttcattg gtcttttcgg tggtagtgac gacaacaacc
     6841 gtaccatcgc cgttgaaggt agcgctcgaa ttggtggtgc tattgctgga gctgctccct
     6901 gtcgggttgc cgttggcgtc tttggtcgag ctgttagagc tggaagacga attgtttttc
     6961 gtggtgacag gttgcgagcc ttgagagcca gcggaaacgc aggaggtaac accacccaca
     7021 ctgataaagc ttttgccttg gctcatgcaa ttagcctcag gggttaaggc cgtgttattg
     7081 gtggcagccg agcaagcagc cccggtgcca ttgccatatt gaccaacaca agcaccctgc
     7141 ccgttggtga gtttgatgca catatcaacc gcgatggagt aagtgcaatt gctcaggcaa
     7201 tcggtggagc catcggagcc gaagtattta gagccagtga cgccagagac aaaaccggat
     7261 ttgcaagatt cctggcatgt acctgttgtg ggatttcgca cctgaccagc ggcacattgc
     7321 gggactaagc aggtgtgaag aatggtcggc gtgacaccac cggggcaaac ttcgtgggcg
     7381 gtaatattcc cgatatttcc atttttgctg ttacagaaat atttccaatt atcgacctta
     7441 gtgacactgc cataagaaac gcctgaataa gccgtatagg cttggcaaga ttcctcgggt
     7501 gtaggctttt ttgtgacggt catgccatct tttgaccagt aatcaataat ggaggggatt
     7561 tcttcattgg ccgcatgcgc atagctactc agcaccattg aaagtaggag aatcaggcgg
     7621 agaaaatgag ccatagcgcc cccagtaatc cgatcaacac taaccatcct tccattttcc
     7681 gtaccccctt aagaaaaaag gcccggaggg cttagaagct ccgggccgat tggtcttgat
     7741 cgtggaatta accccggatc atgctgtata ccttcttgat ggcccagacc accaagatgg
     7801 cgccaccgat cgcagctaca gccgtttcac cttcaccgag tttttcggta acagcggaaa
     7861 cgtcgatagc ggcttgggct tgtgcagcag ccaaggtcat accaacagcc aggtaagcgg
     7921 caactttgcg ggaatacagg ttaactttct tcatgttgag actccttttg tttaagtaat
     7981 gaaatgagga cgcggaaaac cgctccgatt gcccacagta gcgctatggc agccgctagc
     8041 tgttgaccct gttcgactgt cagggaccat gccccggcgg tcagttcttt tggctgtgca
     8101 atgaggtata cacactctga aatctcggta ggttgcgggc tggcgatctg gtattgcccg
     8161 gctccgatat cggttaagca ttcggcggcg taggcgtctg tcattcccca gatgaacgtg
     8221 aggaatccgc cgagcaaaag gcccataatc agcgccatgg cattgacctg gctgcgtgaa
     8281 agatgaatgg aggcgatccg attaaaggct ttcactgatc gaacctcgca atatgagtat
     8341 gtccaggtct ttcgtggccc atatctggta atcaaagacg cccatcaaat cggcgatgcc
     8401 tagcgtgtgt tgggtggcca ccgggaacgt ctgttcttta tggatggcag cgaatgcgcc
     8461 gaggtattgg cgcatgacct tgggcgcggg tatgccccgg cttgctttat agcggcgctc
     8521 gcccaattcg cggctatggg cgaatgtctt gctgatgtac ttgcttaaat aagcggcgag
     8581 ctgcaagcat gtgaaaatcg gcgactgacc agagaagcgc tttttacggt agcgcacatt
     8641 gatctgacct ttggtgttct cgcctgcatc gtctgcattg ccaccgatgg cgatatacca
     8701 gcacttgcgg aggtggtgga catcctgttt accgacgcag gcgacgtgca tatggagtgc
     8761 gccgcgttcc tggtattcct tgacgccgac atagagccaa tcggggtatt tttcgcgaac
     8821 gaggcgcacg aagcgggtga agtcttcgtt cagtttgtcg ctatcggtca tgttctcgcg
     8881 atagaccaac gtcaggaggt gatcggcccc gatggactgg atcagaaagc ggactttttg
     8941 ccgggcgcgg cgttcggcac ggcgaaggtt ttcttcctgg gaggcttcga tttcctcttc
     9001 ggttttttgg cgtggcaagc ccctgatccc atgcgagcgg gatttccgta gctcgtccat
     9061 aaagttgaca cgcctttgta tcatttccac cattcctgag gcgaactccc tgattgttaa
     9121 gtgataatcg gggagctccg tatatacatg atcctgatct agtgctaagc ttgtacccat
     9181 gttttagcca tccgcttatg gttaaaaata gaaagccctg attggttgcc gcctttcagg
     9241 gcttttgttt tatgccttta cctgttcccg tgcatgctct ctggtctgac tgattggggt
     9301 gagtttttcg atacgaggtt gcagccgtcc ctgatagtta ctccagccga tagacgccat
     9361 ataaaagcca gcctcaggct tgaattccgg gtcataactt gggatgactc ctactacctg
     9421 atctacgaag cattgcagat cgtggcggac gtattccccg cgttcacttt tgccagtgcg
     9481 tgagctgatt gccagtactt gaatcttgag ttccatggtg tgtttccttt agttgattga
     9541 gtgcttatgt acccgctgat tgggtgttaa gcccctacgg tgcgggcgtt gccgagggga
     9601 gtcttattca taaaaggatt gctctaacgc gtttttggtg acgagcgcga ggttgaccag
     9661 gcggtatttg ccgatctcga caacggggag gtagcccttg ttaatccagc ccgtaacggt
     9721 gtcttctgtc acacctacgt gttgggcgaa tttagtgcgg gacatgaggg gcacgacggt
     9781 caattgactt gcgtcgaagt gagtgtccat aatgcg
//
