LOCUS       NC_016079               4050 bp    DNA     circular CON 08-JUN-2013
DEFINITION  Pelagibacterium halotolerans B2 plasmid pPHB2, complete sequence.
ACCESSION   NC_016079
VERSION     NC_016079.1  GI:357383070
DBLINK      Project: 74393
            BioProject: PRJNA74393
KEYWORDS    .
SOURCE      Pelagibacterium halotolerans B2
  ORGANISM  Pelagibacterium halotolerans B2
            Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
            Hyphomicrobiaceae; Pelagibacterium.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Huo,Y.Y., Cheng,H., Han,X.F., Jiang,X.W., Sun,C., Zhang,X.Q.,
            Zhu,X.F., Liu,Y.F., Li,P.F., Ni,P.X. and Wu,M.
  TITLE     Complete Genome Sequence of Pelagibacterium halotolerans B2T
  JOURNAL   J. Bacteriol. 194 (1), 197-198 (2012)
   PUBMED   22156395
REFERENCE   2  (bases 1 to 4050)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2011) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 4050)
  AUTHORS   Huo,Y.-Y., Cheng,H. and Wu,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2011) College of Life Sciences, Zhejiang
            University, Hangzhou, China
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence is identical to CP003076.
            Source DNA/bacteria is available from Wu Min (wumin@zju.edu.cn).
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Pelagibacterium halotolerans B2"
                     /mol_type="genomic DNA"
                     /strain="B2"
                     /culture_collection="CGMCC:1.7692"
                     /db_xref="taxon:1082931"
                     /plasmid="pPHB2"
     gene            complement(90..854)
                     /locus_tag="KKY_p1"
                     /db_xref="GeneID:11258754"
     CDS             complement(90..854)
                     /locus_tag="KKY_p1"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="YP_004901677.1"
                     /db_xref="GI:357383071"
                     /db_xref="GO:0004316"
                     /db_xref="GeneID:11258754"
                     /translation="MTLKPLDGKVALVTGGSRGLGREICCALAGLGVRVGVNFSQNET
                     AAAETVRRIERARGIAFAVQGDVTAEGDARRAVDRVTERFNGNVDILVNNANGQHPRY
                     SVEESDWEAYLVQLQYTVKAPLQLLKAVVGPMKTKGSGSIINIGSEVVQVGDANFSSY
                     VTAKSAMLGMTRSWARELASSGIRVNLVAPGFIPVERHRDTESQIIDAYRATVPLGRM
                     GQAGDVASAVSFFASDAAGFITGQCLSVNGGRTFGI"
     misc_feature    complement(108..839)
                     /locus_tag="KKY_p1"
                     /note="3-ketoacyl-(acyl-carrier-protein) reductase;
                     Validated; Region: fabG; PRK05557"
                     /db_xref="CDD:235500"
     misc_feature    complement(99..830)
                     /locus_tag="KKY_p1"
                     /note="Rossmann-fold NAD(P)(+)-binding proteins; Region:
                     NADB_Rossmann; cl09931"
                     /db_xref="CDD:245206"
     misc_feature    complement(order(276..287,363..365,375..377,414..422,
                     567..575,732..740,795..797,801..806,810..812))
                     /locus_tag="KKY_p1"
                     /note="NAD(P) binding site [chemical binding]; other site"
                     /db_xref="CDD:187535"
     misc_feature    complement(order(363..365,375..377,414..416,498..500))
                     /locus_tag="KKY_p1"
                     /note="active site"
                     /db_xref="CDD:187535"
     gene            complement(860..2452)
                     /locus_tag="KKY_p2"
                     /db_xref="GeneID:11258755"
     CDS             complement(860..2452)
                     /locus_tag="KKY_p2"
                     /codon_start=1
                     /transl_table=11
                     /product="Beta-xylosidase"
                     /protein_id="YP_004901678.1"
                     /db_xref="GI:357383072"
                     /db_xref="GO:0009044"
                     /db_xref="GeneID:11258755"
                     /translation="MARQIDNPVLRGFNPDPSFLRVGQDYYIATSTFEWWPGVAIHHS
                     KDLVNWRLLTHALTRQSQLDMTGNPNSGGIWAPCLSYADGIFYLIYTDVKSRRGAFKD
                     THNYLVTASSIEGPWSDPVYLNSSGFDPSLYHGHDDRKWLLNMVWDHRQGRNRFGGIV
                     LQEYSVAERRLVGRPETIFLGTNRGFTEGPHLYYHDGFYHLMVAEGGTKYDHCVTMAR
                     ARDITGPYEVDPLGSMLTSADDRSLPLQKSGHGSLIETHTGEWYLAHLCARPVVDHYC
                     ILGRETALQRCSWSADGWLRLAQGENKPLVEVAPPALPAVPFDPVPDTVFFATKVLGQ
                     HWSTLRSPPDPSWMTFDERDGFLRLYGRESLSSMHRQSLVARRQQAFDFEAEIGVDFA
                     PENFQQMAGLVLFYDTDDYVYLRITSDEKAGKILGIVRSVAGAYEELPESDVVLTARS
                     LCRLKAEVTGPTLKFSYALGDSEWTRIGPQLDIRNLSDDFAEPVRFTGNFIGMCVQDL
                     AGTRAHADFEYFSYRELNHSSE"
     misc_feature    complement(878..2452)
                     /locus_tag="KKY_p2"
                     /note="Beta-xylosidase [Carbohydrate transport and
                     metabolism]; Region: XynB; COG3507"
                     /db_xref="CDD:226038"
     misc_feature    complement(1562..2434)
                     /locus_tag="KKY_p2"
                     /note="Glycosyl hydrolase family 43, beta-D-xylosidase;
                     Region: GH43_XYL_1; cd09000"
                     /db_xref="CDD:185741"
     misc_feature    complement(order(1613..1615,1703..1705,1829..1834,
                     1889..1891,2066..2071,2225..2230,2354..2356,2405..2407))
                     /locus_tag="KKY_p2"
                     /note="inhibitor binding site; inhibition site"
                     /db_xref="CDD:185741"
     misc_feature    complement(order(1889..1891,2066..2068,2405..2407))
                     /locus_tag="KKY_p2"
                     /note="active site"
                     /db_xref="CDD:185741"
     gene            complement(2456..4045)
                     /locus_tag="KKY_p3"
                     /db_xref="GeneID:11258756"
     CDS             complement(2456..4045)
                     /locus_tag="KKY_p3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_004901679.1"
                     /db_xref="GI:357383073"
                     /db_xref="GeneID:11258756"
                     /translation="MKRFEMNALVFHQTDMMDFITLPEKYFSEDLLWERWPVRYIDVQ
                     VKRAFLQEVTRRCKAQGVAFYPEFKEIWYPEGLLEVYPELKNKDGQICPTNPFWFEFV
                     AEKMDELVRYYPDVAGAIVSPATRESKVSISKMTCDCERCRSTDPNKWYEDFIRAVYK
                     PLEACGKTLVIRDFSYSKDHQNAVIEASMAVSKDIVIGLKNVPHDFWPTFPDNPKLGQ
                     TQGLAQWAEFDVWGQYYGQGAFPASLVEDFQNRIRHCRSKGVTGAWFRTDIEWLDESN
                     NMNSFNTLNLIGAAMLSSNPDRDLDEVYDAWLEWGLYSGFTASSETGEPVRPSAPDAR
                     ERLQAFMRAGSKVMEKALYVRGHVFNYSSQFGQSLSAIYNVMQVWHQREQWDPGSAKH
                     LEPTPENLAVIYAEKREAVEQAEKLKDILDPGTLGLPDAFLVEIEAMLELYVWYARGY
                     ELSAHAYFAAQRALQTGERSDAVTALANADALDAFQGELASHLETTVYPHYVYWLTSP
                     TRLHQLAEDVRSKVNSTSEIA"
     misc_feature    complement(<3701..4045)
                     /locus_tag="KKY_p3"
                     /note="Beta-N-acetylhexosaminidases of glycosyl hydrolase
                     family 20 (GH20) catalyze the removal of beta-1,4-linked
                     N-acetyl-D-hexosamine residues from the non-reducing ends
                     of N-acetyl-beta-D-hexosaminides including
                     N-acetylglucosides and N-acetylgalactosides; Region:
                     GH20_hexosaminidase; cl02948"
                     /db_xref="CDD:207794"
ORIGIN      
        1 ctaaagcctg atcaacgtcc ttccaattcc acatccgccg gttgtggaac tcgataccaa
       61 tggttttcac gaacaactct ctctgtggct cagattccaa acgtccggcc gccattgacc
      121 gacaaacact gacccgtaat gaaaccggca gcgtccgagg caaaaaacga aacggcactg
      181 gcgacatcgc ccgcctgtcc catgcggccg agcggtacag tggcccgata agcgtcgatg
      241 atctggcttt cggtgtcgcg atggcgttca acgggaataa accctggtgc cactaggttc
      301 acccggatac ccgaagatgc cagttcccgc gcccaagatc gcgtcattcc cagcattgcc
      361 gatttcgctg tcacatagct ggagaaatta gcatcgccga cctgaacgac ttcgctgccg
      421 atgttgatga tacttccgct gccctttgtc ttcatcggac cgacgacggc tttgagcagt
      481 tgcaacggcg cttttacggt atactgcaac tgcaccagat atgcttccca gtcgctctcc
      541 tcgaccgaat agcgcggatg ctgtccgttg gcattgttaa ccagaatgtc aacattgccg
      601 ttgaaacgct cagttactct gtccactgcc cgtcgtgcat cgccttccgc ggttacatca
      661 ccttggactg cgaatgcaat tccgcgggcc cgttctattc gtcggacagt ctcggctgca
      721 gcggtttcat tctgtgagaa gttgacgccg acccgtaccc caaggccggc tagcgcgcaa
      781 caaatttcac gccccagccc tctggatcct ccggttacca aggccacttt tccatccaat
      841 ggtttcaatg tcatggtcct cactcgctcg aatggttaag ctcgcgatag ctgaagtact
      901 caaagtcggc atgcgcacgc gttccggcca agtcctgaac acacatgccg atgaagttac
      961 cggtgaaccg taccggctca gcaaaatcgt ccgaaagatt tcgtatatcg agttgcggcc
     1021 ctatacgcgt ccattcggag tcaccaagag cataagaaaa tttcaacgtc ggccctgtca
     1081 cttccgcttt caatcgacac agggagcgag ccgtcaggac gacatcgctt tcgggtaatt
     1141 cctcataagc gcctgcgacg ctgcggacta tgccgaggat tttgcctgct ttttcatcgc
     1201 tggtaattct gagatagacg tagtcgtcgg tatcatagaa cagaaccaac ccggccattt
     1261 gctgaaagtt ttccggtgcg aaatcgacac caatctcggc ctcgaaatca aacgcctgct
     1321 ggcgccgggc cacaagactt tggcgatgca tcgaactgag cgattcccgt ccatagagac
     1381 gcaggaaacc gtcacgctcg tcgaaggtca tccacgaggg atcagggggg cttctcaggg
     1441 tgctccaatg ctgaccaaga acctttgtag caaagaaaac ggtatcgggt acaggatcaa
     1501 agggaacggc aggtagtgct ggcggcgcaa cttcgacaag cggcttattt tcaccttgag
     1561 caagtctaag ccagccatcc gcgctccaag aacaacgctg cagggctgtt tctcttccaa
     1621 gtatacagta gtggtcaacg accggccgag cacataaatg cgccaggtac cattcgcctg
     1681 tatgtgtttc gatcaaagag ccatgtcccg acttttgcag cggtagtgag cgatcgtcgg
     1741 cactggtcag catcgaacca agaggatcga cttcgtaggg gccggtgatg tcgcgcgcgc
     1801 gcgccatggt gacgcagtga tcgtattttg taccgccttc cgccaccatg agatggtaga
     1861 acccgtcgtg gtaatagagg tgggggcctt cggtgaaccc acgatttgtg cccaaaaaaa
     1921 tcgtttcggg gcgacccacc aatcgtcgct ccgccaccga atactcctgc agtacaattc
     1981 cgccgaaccg atttcgcccc tgcctgtgat cccagaccat gttgagcagc cattttcgat
     2041 cgtcatggcc atgatacaga gatgggtcga accccgaact gttcaggtag accggatcgg
     2101 accacgggcc ttctatactg gaagccgtca ccaggtaatt atgagtatct ttgaaggcgc
     2161 cacgtcgcga cttaacgtcc gtgtaaatga gatagaagat gccgtccgca tacgaaaggc
     2221 acggcgccca gatccccccg gaattgggat ttcctgtcat gtcgagctgg ctttgccgcg
     2281 tcagagcatg cgtgagcaaa cgccagttca ccaggtcttt agaatggtgg atggccacac
     2341 cgggccacca ctcgaaggta gacgttgcga tgtagtagtc ttgcccgacc cgtaaaaacg
     2401 acgggtcggg gttaaagcca cgcaacacgg gattgtcgat ttgtctcgcc atgtttcagg
     2461 cgatttcgct tgtagaattg accttgctgc ggacgtcctc ggcgagttga tggaggcggg
     2521 ttggactggt cagccaatag acgtagtgag gatagaccgt ggtctcaagg tggctggcga
     2581 gttcgccttg aaaagcgtcc agggcatctg cgttggcaag ggcggtcacg gcgtcactgc
     2641 gttcgccggt ctgcagggcc cgctgggccg caaaataagc gtgcgcggac agctcatagc
     2701 cccgggcata ccagacatag agctctagca tagcctcgat ctcgaccagg aacgcgtccg
     2761 gcagaccgag cgtgccggga tcaagaatat ccttgagctt ttccgcctgc tcaaccgctt
     2821 cacgcttttc agcatagatg acggcaaggt tctccggggt cggctcgaga tgtttcgccg
     2881 agccagggtc ccattgctcg cgctggtgcc agacctgcat cacgttatag atggcgctca
     2941 gcgactggcc gaactggctc gagtaattaa agacgtgccc acgcacataa agggcctttt
     3001 ccataacctt tgaccctgca cgcatgaagg cctgcagccg ttcgcgcgca tccggagcgc
     3061 tcggccggac aggctcgccg gtttcgctcg aagcggtaaa tcccgaatac agcccccact
     3121 cgagccaagc atcgtagacc tcatcgagat cgcgatcggg attggaactc agcatcgccg
     3181 caccaatcag gttgagcgta ttgaagctgt tcatgttgtt gctctcgtcg agccattcga
     3241 tatcggtgcg gaaccaggcg cccgttaccc ctttggaccg gcagtgacga atgcggttct
     3301 ggaagtcctc caccagactg gcgggaaacg ccccctgccc gtaatactgc ccccaaacgt
     3361 cgaactcggc ccattgcgcc aagccctggg tttggcccag cttgggattg tcgggaaacg
     3421 tcggccagaa gtcgtggggc acattcttga gcccgatcac gatgtctttc gataccgcca
     3481 tgctggcttc tatgacggca ttctgatgat ccttggaata ggaaaaatca cggatcacca
     3541 gcgtcttgcc gcacgcttca agcggcttgt aaacagcgcg gatgaaatcc tcataccatt
     3601 tgttcggatc cgtgctgcgg caacgctcgc aatcgcaggt catcttggag atggacacct
     3661 tgctctcgcg ggtggccggg ctgacgatcg ccccggccac gtcggggtaa tagcgcacca
     3721 gctcgtccat cttttcggcc acgaactcaa accaaaacgg attggtggga cagatctggc
     3781 catccttgtt cttgagctcg ggatacacct ccagcagacc ttcaggatac cagatctcct
     3841 tgaactccgg ataaaaggcc accccttgcg ccttgcagcg ccgcgtcacc tcctgaagaa
     3901 aagcccgctt gacctgaacg tcgatatagc gaaccggcca ccgctcccac agcaggtcct
     3961 cgctaaaata cttctcgggc agcgtgatga aatccatcat atccgtctga tggaaaacaa
     4021 gagcgttcat ttcaaaacgc ttcatgaaag
//
