LOCUS       NC_019141               5718 bp    DNA     circular BCT 03-NOV-2012
DEFINITION  Staphylococcus aureus plasmid pCPS32, complete sequence.
ACCESSION   NC_019141
VERSION     NC_019141.1  GI:410655963
DBLINK      Project: 178791
            BioProject: PRJNA178791
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacillales; Staphylococcus.
REFERENCE   1
  AUTHORS   Kadlec,K., Pomba,C.F., Couto,N. and Schwarz,S.
  TITLE     Small plasmids carrying vga(A) or vga(C) genes mediate resistance
            to lincosamides, pleuromutilins and streptogramin A antibiotics in
            methicillin-resistant Staphylococcus aureus ST398 from swine
  JOURNAL   J. Antimicrob. Chemother. 65 (12), 2692-2693 (2010)
   PUBMED   20876620
REFERENCE   2  (bases 1 to 5718)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2012) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 5718)
  AUTHORS   Schwarz,S.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2010) Schwarz S.P., Molecular Microbiology &
            Antibiotics, Institute of Farm Animal Genetics (FLI), Hoeltystr.
            10, 31535 Neustadt-Mariensee, GERMANY
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence is identical to FN806791.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /isolate="E32"
                     /isolation_source="swine with MRSA isolate E32"
                     /host="Sus scrofa"
                     /db_xref="taxon:1280"
                     /plasmid="pCPS32"
                     /country="Portugal"
     gene            799..1737
                     /gene="rep"
                     /locus_tag="D646_p2002"
                     /db_xref="GeneID:13913663"
     CDS             799..1737
                     /gene="rep"
                     /locus_tag="D646_p2002"
                     /function="involved in plasmid replication"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="YP_006958104.1"
                     /db_xref="GI:410655964"
                     /db_xref="GeneID:13913663"
                     /translation="MSENIQENHSKQGTSGEVTNYTRTGYSNSRLDAHFLCTSHPKLS
                     FDGMTIVGNLNKNNAERLSKFMSIEPQIRMWDILQTKFKAKALQEKVYIEYDKVKADS
                     WDRRNMRVEFNPNKLSHDEMVWLKRNIINLMEDDGFTRLDLAFDFEDDLSDYYAMSDK
                     ALKKTVFYGIDGKPETKYFGVRDSERFIRIYNKKKERKDNADIEIDSEHLWRVEIELK
                     RNMVDYWNNCFDDLHILKPAWATLESVREQAMVYLLLHEESAWGELHRNSRRKYKQLL
                     QEISPIDLTNLMKLALKENEKQLQKQIDFWLSDFRF"
     gene            complement(2059..3627)
                     /gene="vga(A)"
                     /locus_tag="D646_p2001"
                     /db_xref="GeneID:13913661"
     CDS             complement(2059..3627)
                     /gene="vga(A)"
                     /locus_tag="D646_p2001"
                     /function="confers resistance to streptogramin
                     A,lincosamides and pleuromutilins"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="YP_006958105.1"
                     /db_xref="GI:410655965"
                     /db_xref="GeneID:13913661"
                     /translation="MKILLEGLHIKHYVQDRLLLNINRLKIYQNDRIGLIGKNGSGKT
                     TLLHILYKKIVPEEGIVKQFSHCELIPQLKLIESTKSGGEVTRNYIRQALDKNPELLL
                     ADEPTTNLDNNYIEKLEQDLKNWHGAFIIVSHDRAFLDNLCTTIWEIDEGRITEYKGN
                     YSNYVEQKELERHREELEYEKYEKEKKRLEKAINIKEQKAQRATKKPKNLSSSEGKIK
                     GTKPYFASKQKKLRKTVKSLETRLEKLERVEKRNELPPLKMDLVNLESVKNRTIIRGE
                     DVSGTIEGRVLWKAKSFSIRGGDKMAIIGSNGTGKTTFIKKIVHGNPGISLSPSVKIG
                     YFSQKIDTLELDKSILENVQSSSQQNETLIRTILARMHFFRDDVYKPISVLSGGERVK
                     VALTKVFLSEVNTLVLDEPTNFLDMEAIEAFESLLKEYNGSIIFVSHDRKFIEKVATR
                     IMTIDNKEIKIFDGTYEQFKQAEKPTRNIKEDKKLLLETKITEVLSRLSIEPSEELEQ
                     EFQNLINEKRNLDK"
     gene            4549..5526
                     /gene="mob"
                     /locus_tag="D646_p2003"
                     /db_xref="GeneID:13913662"
     CDS             4549..5526
                     /gene="mob"
                     /locus_tag="D646_p2003"
                     /function="involved in plasmid mobilization"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="YP_006958106.1"
                     /db_xref="GI:410655966"
                     /db_xref="GeneID:13913662"
                     /translation="MATTKISSTKSTSRAINYAEKRAEEKSGLNCDVEYAKGSFKAVR
                     ELYNKTNGNEGHVVIQSFKPDEVTPKQCNQLGLELAQKIASNHQVAVYTHADTDHVHN
                     HIVINAVDIETGKKFNNNKKALRDIRNANDQVCREHGLSIPEKQAEIRYTQGEKGRLE
                     RGKTSWKDEVREKIEDSLKDTTDFEQFEKKLKTSGVEITRKTKKTITYKHESEDKKVR
                     GSRLGNNYDLGGVMLELERQNERAKEREEQEREREQERRQEQYADKYKVDWDGFENIA
                     DERRNQRIEKAKQIARKNDDRNRERPREPQSQFGKIDKKIGRDHDGLQL"
ORIGIN      
        1 ggtaggagta tttgcagcat ttgttttatt tgcggtttta agtacgtttt taggtggaat
       61 gatgaacgtt ttcggtgtac ctcagttata cgacaattta aatcacgcta taaagcgcac
      121 agagagcttg tggggcgttt tatggtattt agggtacttg gtaccatatt taatgctcgg
      181 tggctttgta tggctcttat ttgccctctt agagaaatac agataaaaag cttttaacaa
      241 gcatactgaa cttgttaaaa gctatagccg aaattataaa aaagccaatt gattagttct
      301 ttgggaggag ctgcaactcc ttctccattt caattataac aagaacgagc atttatgccg
      361 agaaaattta ttgatcgttg aggagaaccc ttaactaaac tgaaccgacg aatgtcgggg
      421 atagcgtgag ctattacccc gacgagtcga cagttttggg tttgttaagg gttcagaggc
      481 tcaacgtcaa taaagcaatt tggaataaag caacaaaggt ttttgattat gaaacagggt
      541 tttgttacac attgcttttt tcaagcagtt agacccaagc tatgtaagag tgatatatta
      601 taaataaaat aaaaagagag tcgttccact ccctcgccaa agtttgtgaa aacgactcac
      661 ccaataaaaa gatacaaaaa acgtagatgt tttgttttta ttttgtatat cttgatgtta
      721 tacgaattta gacaaatctt caagatataa aattgggtga gcagggtgga acgacttctt
      781 gaaaaggagt cgatttttat gtctgaaaat attcaagaaa atcattcaaa acaaggcaca
      841 tcaggggaag ttacaaatta caccagaacc ggctactcta atagccggtt agacgcacat
      901 ttcttgtgca cttctcatcc aaaattaagt tttgacggga tgactattgt tggaaacctt
      961 aataaaaaca acgcagaacg actttcaaag ttcatgagta ttgaacctca aattagaatg
     1021 tgggatattt tgcaaactaa atttaaagct aaagcattac aagaaaaagt ttatattgag
     1081 tatgacaaag tgaaagcgga tagttgggat agacgtaaca tgcgtgttga atttaatccg
     1141 aacaaacttt cacatgacga aatggtttgg ttaaaacgca atataataaa tcttatggaa
     1201 gatgacggtt ttacaagatt agatttggct tttgattttg aagatgattt aagtgactat
     1261 tatgcaatgt cagataaagc tttaaagaaa actgtatttt acggaattga cggaaagcca
     1321 gaaactaaat attttggtgt acgtgatagt gaacggttta ttagaattta caacaagaaa
     1381 aaggaacgta aggataatgc agatatagaa atagattctg aacatttatg gcgtgtagaa
     1441 attgaattaa aacgtaacat ggttgattac tggaataatt gctttgatga tttgcatatt
     1501 ttaaaaccag catgggctac tttagaaagt gtaagagaac aagcaatggt gtatttatta
     1561 cttcacgagg aaagtgcttg gggagaatta catagaaact cccgtcgcaa atacaaacaa
     1621 ctacttcaag aaatatctcc aattgattta acgaacttaa tgaaattggc tttgaaagaa
     1681 aacgaaaaac aattgcaaaa gcagattgat ttttggcttt ctgattttcg gttttgagtg
     1741 aatttaatct tatactgtga ggtaaaaagc gagcagatta taaaattaat tgaacgtgtt
     1801 gcaaccacca tttttggttg ctaggtttta tggttttgaa ctatgagtgg ctagctttta
     1861 gccactcata gttcaaacga aaaggcacaa acaccgatat gacggtgttt gtgccttttc
     1921 gtttagttaa cataattaac ccttatgggt agttgaattg tatatgttat gcaatacact
     1981 aacttaataa atattaccca tcaatgattc aataaaaaga caacaagaat aatctttatt
     2041 gcttaaattt aaagtaattt atttatccaa atttcttttt tcatttatta agttttgaaa
     2101 ctcttgttct aattcttccg aaggttcaat actcaatcga ctgagtactt ctgtaatttt
     2161 tgtctcaagt aaaagttttt tatcttcttt aatattcctt gttggctttt cagcttgttt
     2221 aaattgttca tatgtgccat caaatatttt tatttcttta ttatcaattg tcattattcg
     2281 agtggctact ttttcgataa atttacgatc gtgagataca aaaattatac tgccattata
     2341 ttcctttaac aaagattcaa acgcctctat agcttccata tcaagaaagt ttgttggttc
     2401 atctagtacc aacgtattaa cttcacttaa gaatacttta gttagtgcta ctttaactcg
     2461 ctctccacca cttaagacac ttattggttt ataaacatca tctctaaaaa aatgcattct
     2521 agctagaata gttcgaataa gagtttcatt ttgttgtgaa gaagattgaa cattttctaa
     2581 aatgctctta tctaattcta atgtatctat tttttggcta aaataaccga ttttgacaga
     2641 tggcgataat gaaataccag gatttccatg cacaattttt ttaataaacg ttgtctttcc
     2701 tgtaccatta gatccgataa ttgccatctt gtctcctccg cgaatactaa aactttttgc
     2761 tttccacaat acccgtcctt caattgtacc cgagacatct tcaccacgta ttatagttct
     2821 attttttaca ctttctaagt tcactaaatc cattttaagt ggaggaagtt cgtttctctt
     2881 ttcgaccctt tcaagttttt ctagtctggt ttctagagat tttacagttt ttcgtaactt
     2941 cttttgctta cttgcaaagt atggctttgt tccttttatt ttgccttcag atgaacttaa
     3001 gtttttcggt tttttagttg ctcgttgagc tttctgttct tttatattta tagctttttc
     3061 caatcgtttc ttttcttttt catatttttc atattctaat tcttctcgat gtctttctaa
     3121 ttctttttgt tcaacatagt tactataatt ccccttatat tcagttattc tcccctcgtc
     3181 aatttcccat atagtagtac ataagttatc taaaaaagcg cgatcatgtg aaactataat
     3241 aaatgctcca tgccaatttt ttaaatcctg ttctaatttt tctatatagt tattatctaa
     3301 gttagttgtt ggttcatctg ctaatagcag ttctggattt ttatcaagcg cttgccgaat
     3361 atagtttcgt gttacttcac caccactttt agttgattct atgagcttca attgaggaat
     3421 aagttcacaa tgtgaaaatt gttttacaat accttcttca ggcacaattt ttttatataa
     3481 tatgtgaagt aacgttgttt ttccacttcc atttttacca attaaaccaa tacgatcatt
     3541 ctgataaatc tttaggcgat ttatgttcaa caataaacga tcttgaacat aatgttttat
     3601 atgaagtccc tctaacagta ttttcatatt ttctcatcct cttataagta atttaagaca
     3661 ataaaagcct cccaatctat tcagaatagg aggcataaat ctatatagat gaactctaac
     3721 aaaatacttt tagagccaac tacagaattt tcatcctatc ctgaaaagta caattgaaaa
     3781 caacacaaat caaacgctgt ttgtaaataa aacactttca attgtttact tttgaaaata
     3841 ggattagtta atcatgtagt tggttcaccc tttcctttat cgtattaaat tcaatttaaa
     3901 caaatagcaa ataaatgtca atgttcaact caaattattt ttttaattta tttatatcac
     3961 ttaatgaaca gtggctagca attagccact cattttttgc gtaagcaaaa acataaaggg
     4021 tatgggatat aatcccatca agtacggcgt gctagaacga agtggatagc aaagccagtg
     4081 cttgccaaac ctataaaata cttgaagagt tagagcaaat attataagct ctaactgtgg
     4141 aaagtatttg ttttcgacag aaaaaggagg tgggattatg agcgaacgaa ataaaaattt
     4201 ggctagcgat ttgcctgtcg ggaaaaaccg taaacccgac cgcaaagagc cgaaacaaat
     4261 tagttttcgt gtgagcgaat ccgaatatga aaagttgaag cgttcagctg aaaatttgca
     4321 tatgagtgtg cctgctttcg ttaagaaaaa ggcacaaggg gcgagattgg tagcaccgaa
     4381 atttgacgca gaaacgtcaa aagaattggt gcaacaattg agtaagttag ggggcaacgt
     4441 gaaccagatt gcaaagtatt gtaacgagag acgccaacat aatgaagacc ctagattttt
     4501 cgacttggaa agaaacttaa gcattatcag aaaggaactt aaagagatat ggcaacaact
     4561 aaaataagtt caacaaaatc aacatcaaga gcaatcaact atgcggaaaa gcgtgcagaa
     4621 gagaaaagcg gtctgaattg tgatgtggaa tatgccaaag gcagtttcaa agcagtacga
     4681 gaactttata acaaaacaaa tggcaatgaa ggtcacgtag ttattcagtc attcaagcca
     4741 gatgaggtta cgccgaaaca atgtaatcaa cttggattgg aattggcaca gaagatagca
     4801 tctaatcatc aagtagcagt ttatacacat gcagatacag accatgtaca caatcatatt
     4861 gtgattaatg cggtggatat agagacaggc aaaaaattca ataacaacaa aaaggctttg
     4921 cgtgatatta gaaacgcaaa cgatcaggtg tgtcgtgaac atggattatc cataccagaa
     4981 aaacaagcag agataagata cacgcaaggc gaaaaaggca gacttgaaag aggtaaaaca
     5041 tcttggaaag acgaagtcag agaaaagatt gaggatagtt tgaaagacac aacagacttt
     5101 gaacagtttg agaaaaagtt gaaaacaagc ggtgttgaaa taacacgtaa aaccaaaaag
     5161 acgattactt acaagcacga atcagaagat aaaaaagtgc gtggcagtcg attaggcaac
     5221 aattacgatt taggaggtgt catgcttgag cttgaaagac agaatgaacg agcaaaggaa
     5281 cgagaggaac aagagcgaga gcgagaacag gaacgcagac aagaacagta cgcagataaa
     5341 tacaaggtcg actgggacgg attcgaaaac attgcagatg aacgccgaaa tcaaagaatt
     5401 gaaaaggcaa aacaaattgc tcgcaaaaat gatgatagaa atagagaacg accaagagaa
     5461 ccgcaatcgc aatttggaaa aattgacaaa aaaattggac gagaccacga cggattacag
     5521 ttataaagcc gaaactgcac aaaccaagta tttaaaattt ttaaatgaca gactaaagca
     5581 gattgatagc gataaagtaa aagagaagat gttgaaagaa tacaatgact tagcacactt
     5641 taaccaacaa gcgaaagaag aaatcaaaca agcacatgaa cgtaatgaaa gtgtgcagaa
     5701 gtggtttaaa tttagttt
//
