LOCUS       NC_019142               5292 bp    DNA     circular BCT 03-NOV-2012
DEFINITION  Staphylococcus aureus plasmid pCPS49, complete sequence.
ACCESSION   NC_019142
VERSION     NC_019142.1  GI:410655967
DBLINK      Project: 178791
            BioProject: PRJNA178791
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacillales; Staphylococcus.
REFERENCE   1
  AUTHORS   Kadlec,K., Pomba,C.F., Couto,N. and Schwarz,S.
  TITLE     Small plasmids carrying vga(A) or vga(C) genes mediate resistance
            to lincosamides, pleuromutilins and streptogramin A antibiotics in
            methicillin-resistant Staphylococcus aureus ST398 from swine
  JOURNAL   J. Antimicrob. Chemother. 65 (12), 2692-2693 (2010)
   PUBMED   20876620
REFERENCE   2  (bases 1 to 5292)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2012) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 5292)
  AUTHORS   Schwarz,S.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2010) Schwarz S.P., Molecular Microbiology &
            Antibiotics, Institute of Farm Animal Genetics (FLI), Hoeltystr.
            10, 31535 Neustadt-Mariensee, GERMANY
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence is identical to FN806792.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /isolate="E49"
                     /isolation_source="swine with MRSA isolate E49"
                     /host="Sus scrofa"
                     /db_xref="taxon:1280"
                     /plasmid="pCPS49"
                     /country="Portugal"
     gene            101..1669
                     /gene="vga(C)"
                     /locus_tag="D646_p3001"
                     /db_xref="GeneID:13913668"
     CDS             101..1669
                     /gene="vga(C)"
                     /locus_tag="D646_p3001"
                     /function="confers resistance to streptogramin
                     A,lincosamides and pleuromutilins"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="YP_006958107.1"
                     /db_xref="GI:410655968"
                     /db_xref="GeneID:13913668"
                     /translation="MVLLEAKNIKHYIKDRLLLKIDELKIEHNDRIGLVGVNGSGKTT
                     LLNILAEKFIPDEGTITPYAQSEILPQLKKTDAAKSGGEITQEYIQQTLNSAPALLLA
                     DEPTTNLDTDHIEWVEKKLKRWQGAFVLVSHDREFLDALCSVIWELEDGEITEYKGNY
                     SDYLKQKEVEKQQQQSTYEKYEKEKKQLEKAIQLKEEKAQRATKKPKNLSASERRIKG
                     SKPYFAKKQKKLHKTAGALETRVEKLEKVEKIKDQPPIKMDLPNERNLKNRVIIRVED
                     LEGLVPKQLLWKKATFQIYGGDKLAIIGPNGSGKTTLVKKIITQENGVTISPSVKIGY
                     FSQNLMTLDVNKSILENVQNSSKQEESLIRTVLARMHFFDEDVYKPVHVLSGGERVKV
                     ALTKLLVSDINTLVLDEPTNYLDTEALKALENLLNEYTGSIIFVSHDRTFTENIATRI
                     LEIRNKKIEIFDGTYQQFKNRSTKKERDFQQEEQLLLDTKISEVLSRLSMEPSQALEK
                     EFQNLLKEKSKLKE"
     gene            1972..3201
                     /gene="pre/mob"
                     /locus_tag="D646_p3002"
                     /db_xref="GeneID:13913667"
     CDS             1972..3201
                     /gene="pre/mob"
                     /locus_tag="D646_p3002"
                     /function="involved in plasmid recombination and
                     mobilization"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination/mobilization protein"
                     /protein_id="YP_006958108.1"
                     /db_xref="GI:410655969"
                     /db_xref="GeneID:13913667"
                     /translation="MQKMKADNLVGIGNHNQRRIDNHSNKDIDVDRSHLNYDLVEGRT
                     ENYKRDIEKFINENKASQRATRKDAVLVNEWIISSDRFFFDELDDKETERFFEDAKDF
                     FAERFGDENIRYAQVHLDERTPHMHLGIVPFDDENKLSAKRVFNKAALQEVQEELPKY
                     LNERGFELQRGEKGSDKKHLPVEEYKAYKDAKFDLENEISALEREVKQRKDEVKKLIT
                     GKYGKLDYEKLGVKNQLKTVEVKTGEKNIFGVEKTETKRVKTGNVVMLDENFRLLAKN
                     YEELVAVKRSFEKYLDTDLIKENKALDKVADEYMEMAYKSSDVANELKAENDRLREEN
                     KSLKEQVQSLKQEVKSIYHTVRDILKRAMSNSNSVKGLMSVIAEKVSEDVPKPEFGRL
                     DDQERMRERKRTRGMSR"
     gene            3958..4620
                     /gene="rep"
                     /locus_tag="D646_p3003"
                     /db_xref="GeneID:13913666"
     CDS             3958..4620
                     /gene="rep"
                     /locus_tag="D646_p3003"
                     /function="involved in plasmid replication"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="YP_006958109.1"
                     /db_xref="GI:410655970"
                     /db_xref="GeneID:13913666"
                     /translation="MAKEKSRYFTFLLYPESIPSDWKMKLELIGVPMAISPLHDKDLS
                     TVDGQQYKKAHYHVIYVAKNPVTADSVRKKIKATLGDSSVAKVQTVVHSMENMYLYLT
                     HESKDALAKNKHKYDTKDIKLINNFDIDRYVTLDESEKKELRQTLLEIIDKYKIVNII
                     ELSAFIRERGNEFGIDGMRYVNEVISSTSGIFRLYFDANYQCGYRPVYSKTIDPETGE
                     MR"
ORIGIN      
        1 ggtctctttt ttgtacccct atatggcttt atatccccta ttctgaaaac aagtagggga
       61 tatttttgta tgccctgtaa ttagtaaagg agagggtagt atggttttac tagaggccaa
      121 aaatataaaa cactacatca aagatcgctt attattaaag attgatgaat taaaaattga
      181 acataatgac cggattggct tggtaggtgt aaatggaagt ggaaaaacaa ctttgttaaa
      241 tatccttgct gaaaaattta ttcctgacga ggggacaatc acgccgtatg cccagagtga
      301 gatattgcca cagttaaaaa agaccgatgc ggcaaaaagt gggggcgaaa ttactcagga
      361 atatattcaa caaacgttaa atagtgctcc agctctttta ttagcagacg aaccgacgac
      421 caatctagat acagatcata tcgaatgggt tgaaaaaaaa ttgaagcgtt ggcagggggc
      481 cttcgttctt gtttcccatg accgagaatt tttagacgct ctgtgttctg ttatttggga
      541 actggaagat ggtgaaataa cggagtacaa gggcaattat agtgattatc tcaagcaaaa
      601 agaagtagaa aagcaacaac aacaatccac atatgaaaaa tatgaaaaag aaaaaaaaca
      661 gctggagaaa gcaatccaac taaaagaaga aaaggcccaa cgagcaacta aaaagcctaa
      721 aaatttatct gcttctgaga gacgaataaa gggatcgaag ccttacttcg caaaaaaaca
      781 gaaaaaactg cataaaactg caggtgctct tgaaacaaga gtagagaaat tagaaaaagt
      841 agagaaaata aaagatcagc cacctattaa aatggattta ccaaatgaaa gaaatcttaa
      901 aaatcgtgtg attattcggg ttgaggatct agaaggactt gttcccaagc agttactttg
      961 gaaaaaggca acttttcaaa tttatggtgg ggacaaacta gctatcatag gacccaatgg
     1021 tagtggaaaa acaactttag ttaaaaaaat aataactcaa gaaaatggtg tcactatttc
     1081 tccgtctgtt aagatcggtt actttagtca gaatctaatg acgctagatg taaacaaatc
     1141 tattttagag aacgttcaaa attcttcgaa acaagaagaa tcactgattc gaacggtact
     1201 agctagaatg catttctttg atgaagatgt atacaaacct gtgcatgtgc taagcggtgg
     1261 tgaaagagta aaagttgcac tgacaaaact attagtgagc gatatcaata cactcgtttt
     1321 agatgaacca acgaattact tggatacgga agcactcaaa gcattagaaa acctcttgaa
     1381 tgaatatacg ggaagtatta tttttgtttc tcatgatcgt acctttaccg aaaatatcgc
     1441 tactcgaatt ttagaaatac gtaataaaaa aattgagata tttgatggga cgtaccaaca
     1501 attcaaaaat agatctacta aaaaggaacg ggattttcag caagaagagc agttactact
     1561 agatacaaag atatccgaag tacttagccg cttaagtatg gaaccttccc aagctttgga
     1621 aaaagaattt caaaacctcc taaaagaaaa aagtaagtta aaggagtaag ttattttcta
     1681 tgtacggttc gaagattaat ataatttttt aagtatttaa agaagccccc tgccaaaagg
     1741 caaaggggga tttttttgtt ttgcacgaaa aaaacaggcg gggattctgc cgtttttcgt
     1801 gcctgttcac gtcgaacctt ttcagcctaa aggcgaaaaa aggggcagag aggacagacg
     1861 gttttcagcc tcgcagagcg cacaccttac tcgtaaggta tagtgtgtta tactttacgt
     1921 ggaagttatg ccggaaagga cgtgcggaca ttgagttatg tggttgcaag aatgcagaag
     1981 atgaaagccg ataatttggt cggaatcgga aaccataatc aaagaagaat agacaatcat
     2041 tccaacaaag atatcgacgt tgaccgctca catttgaatt acgatttagt cgaagggcgc
     2101 accgaaaatt ataagagaga tattgaaaag tttatcaacg agaataaggc aagccagcga
     2161 gcgactcgaa aagacgctgt gcttgtaaac gaatggatca tttcgagtga ccgatttttc
     2221 tttgacgaac tggacgataa agaaacagaa cgattttttg aagatgctaa agattttttt
     2281 gccgaacgat tcggcgatga gaatattcgc tatgctcaag tccatttaga cgaacggact
     2341 ccacacatgc atttgggaat tgttccgttt gacgatgaaa acaaattgtc agcaaaaaga
     2401 gtcttcaaca aagctgctct gcaagaagtc caagaggaat tgcctaaata tttgaatgag
     2461 cgtggttttg agctgcagag gggcgaaaag ggatccgata agaagcattt gccagttgag
     2521 gaatacaaag cctataaaga cgctaaattt gatttagaaa acgaaattag cgccttagag
     2581 cgagaggtaa agcagagaaa agacgaagtg aagaaattaa ttactggaaa atatggaaag
     2641 ttggattacg agaagctcgg agttaaaaat cagctgaaaa ccgtagaagt aaagacaggc
     2701 gaaaaaaata ttttcggagt ggaaaaaact gaaacgaaac gtgtgaaaac agggaatgta
     2761 gttatgcttg atgaaaattt taggcttctg gctaaaaatt atgaggaact agttgccgtt
     2821 aaaagatcat ttgagaagta tttagacact gacttgatta aagagaataa agctttagat
     2881 aaagttgctg atgagtacat ggagatggcg tataaatctt ctgatgtggc taacgagctg
     2941 aaagcagaaa atgacaggct tagagaagaa aacaaatcgc taaaagaaca agtgcaaagt
     3001 ctcaaacaag aggttaaaag tatttatcat actgtcagag acatcttaaa acgcgctatg
     3061 agcaattcta attcggttaa gggtcttatg agtgtaatcg cagagaaagt ctctgaggac
     3121 gttcctaagc ccgaatttgg gcgtttggac gaccaagagc ggatgaggga gcgcaaacga
     3181 accagaggaa tgagccgtta gcggtttttg gcgttacaat agacggtgcg atagtcaaaa
     3241 tctaagtttt tcaactggtc tgactatcgg catgacccgt caaacgccaa aaataaagcc
     3301 gtcaaggtgc tagggtggag atttttcggt ggttttccga aaaatactac ccgatccttg
     3361 acggctttaa gcgttgttta gggcttttca actgggtgac aggaggcgac tgggtggttt
     3421 ggagggtggc aagccctcca aggtcttttt tcttttgttt tttaggaaat tgatgatgtg
     3481 tagctggaaa atgggggggc tactacgacc cccccataag tgccgagtgc ccaaatagac
     3541 tgtaatcacg ttttaagtca tatgtaccaa gggttttaga ccgattatat ctcggcgact
     3601 tttcggcgac ttttcggcga cttttacaaa aaactgtatt tctttgcaat gctttacacg
     3661 gcttaagaaa tgctttatac ttataaataa atattgaata agaaaagagg ttctgcttat
     3721 ggctagagaa aaggaagaaa tgcaaagcgt tactatccgt atccctaaag acttatatgc
     3781 tgattataag aaaaccttat tagcacaagg taaaattgta acttatgacg tgagaaaata
     3841 catgaatgaa gttgtagaaa atcataagaa ggggcaaaaa aatgcataaa aaaagagcgt
     3901 ccgtccaaag accgctctcg aaactaatat gcaatttgtt aaggtgatta tatcattatg
     3961 gctaaagaga aatcaagata cttcacattc ttgctatacc cagaaagcat tccaagtgat
     4021 tggaaaatga agttagaatt aatcggtgtt ccaatggcta tcagtcctct gcatgataaa
     4081 gatttgagta cggttgatgg tcaacagtat aaaaaagctc actatcatgt aatctatgtg
     4141 gcaaaaaatc ctgttactgc agatagtgtt cgtaaaaaaa tcaaagcgac tttgggtgat
     4201 agtagtgtag ctaaggtgca gactgtagtt catagtatgg aaaacatgta cctgtattta
     4261 acgcatgaat cgaaagacgc tcttgcgaaa aataaacaca agtacgatac aaaagatatc
     4321 aagttgatta ataactttga tattgaccgt tatgtcaccc ttgatgaaag cgagaaaaaa
     4381 gaattaagac aaacgttact tgaaattata gataaatata aaatcgtcaa tatcattgag
     4441 ttaagtgcgt ttattaggga acgtgggaat gagtttggga ttgacggaat gagatatgtg
     4501 aatgaagtta tttcaagtac ttctggaatt ttccgtttat actttgacgc aaactatcaa
     4561 tgtggttatc gtccggttta ctcaaaaact attgatccag agacgggaga aatgagataa
     4621 tgcaaaataa aatttatcga gtcttacaaa atgatgaagt ggtcgcaatc tttaatcgta
     4681 aggattacgc aaatgatttt attgattatc aagcaacgat ttcggataaa aaatttgaaa
     4741 ttgaggaagt ttcgttggct gattggttgc ttcagccaag agaattttaa ggataagtga
     4801 gataattaga cgaaatatta gaataaaaaa acttgtccct gctggaaaaa ctaaagagtt
     4861 ctcttatttc ttttggcaaa taaatttgtc aaaaaagtga cacctgattt cgtttactga
     4921 atcaggtaat catgtattga acgagattaa agaaaaagaa ccctattaaa acagggttct
     4981 tgatacatga ttttacccaa attcatgtat ctttgtttta aaatcaatcc attaaaaact
     5041 tttaaaaact ttaatattat aaacactaaa aatggttaag acatgtatat tttgacaaaa
     5101 acatctagtc tatcgtatcc ttatagataa ttactacaac gagagggtga cccaactaca
     5161 tgaaatacta atcctattga cgaaaccaaa caatgaaggc tttacagtag caaaattttg
     5221 ctttttttgt agccttatat tgtctatttt cagaatagga tttgatttct atagttggca
     5281 caaagagata at
//
