LOCUS       NC_019144               7054 bp    DNA     circular BCT 03-NOV-2012
DEFINITION  Staphylococcus aureus plasmid pMSA16, complete sequence.
ACCESSION   NC_019144
VERSION     NC_019144.1  GI:410655974
DBLINK      Project: 178791
            BioProject: PRJNA178791
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacillales; Staphylococcus.
REFERENCE   1  (bases 1 to 7054)
  AUTHORS   Wang,X.M., Zhang,W.J., Schwarz,S., Yu,S.Y., Liu,H., Si,W.,
            Zhang,R.M. and Liu,S.
  TITLE     Methicillin-resistant Staphylococcus aureus ST9 from a case of
            bovine mastitis carries the genes cfr and erm(A) on a small plasmid
  JOURNAL   J. Antimicrob. Chemother. 67 (5), 1287-1289 (2012)
   PUBMED   22334606
REFERENCE   2  (bases 1 to 7054)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2012) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 7054)
  AUTHORS   Wang,X.-M., Schwarz,S., Zhang,W.-J., Yu,S.-Y., Zhang,R.-M. and
            Liu,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2011) Chinese Academy of Agriculture, Harbin
            Veterinary Research Institute, 427 Maduan Street, Harbin,
            Heilongjiang 150001, China
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence is identical to JQ246438.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7054
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="SA16"
                     /isolation_source="fresh raw milk"
                     /db_xref="taxon:1280"
                     /plasmid="pMSA16"
                     /country="China"
     gene            121..1104
                     /gene="rep"
                     /locus_tag="D646_p5003"
                     /db_xref="GeneID:13913677"
     CDS             121..1104
                     /gene="rep"
                     /locus_tag="D646_p5003"
                     /note="essential for plasmid replication"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein"
                     /protein_id="YP_006958112.1"
                     /db_xref="GI:410655975"
                     /db_xref="GeneID:13913677"
                     /translation="MCNNNSINSDEKSSVLIDKTKSGKIRPWREKKMANVDYFELLHI
                     LEFKKAERVKDCAEVLEYKVSYETGEKTLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVRNVYDGTELNKSLSDMSKGFNRMMKYKKINKNLVGFMR
                     ATEVTVNNIDNSYNQHMHVLVCVESTYFYNTENYVNQKQWIQFWKRAMKLDYDPNVKV
                     QMIRPKNKHKSDIKSAIDETAKYPVKDTDFMTDDEERNLQRLADLEEGLYRKRLISYG
                     GLLKEIHKELNLDDAEDGDLIHTDDEEKADEDGFSIIAMWNWERKNYFIKE"
     gene            complement(1177..1908)
                     /gene="erm(A)"
                     /locus_tag="D646_p5001"
                     /db_xref="GeneID:13913675"
     CDS             complement(1177..1908)
                     /gene="erm(A)"
                     /locus_tag="D646_p5001"
                     /note="macrolide resistance"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA methylase Erm(A)"
                     /protein_id="YP_006958113.1"
                     /db_xref="GI:410655976"
                     /db_xref="GeneID:13913675"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            2530..3660
                     /locus_tag="D646_p5004"
                     /db_xref="GeneID:13914265"
     CDS             2530..3660
                     /locus_tag="D646_p5004"
                     /note="delta pre/mob; essential for plasmid recombination
                     and mobilization"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-mobilization protein"
                     /protein_id="YP_006958114.1"
                     /db_xref="GI:410655977"
                     /db_xref="GeneID:13914265"
                     /translation="MDIKYSVLYFTWKLTEIRGRVFVSFAVVRMQKMKSPDLKGMQFH
                     NQRERESRTNPDIDPDREHLNYDLLHQEKIDYNQQVKAIIESQKVSERKTRKDAVLVN
                     ELLVTSDRKFFDGLDPAEQKRFFEESHKLFSERYGKQNIAYATVHNDEKTPHMHLGVV
                     PMRDGKLQGKNVFNRQELQWMQEEFPKHMQSVGFEVERGIASDRKHIEMSRFKALTLN
                     EEIKTLEKETEALRNALTASKKVDELQVTKPSLFDRNHVKLPVEDFEALKARAKATEA
                     IERTIEAHEKRFDDMVDNVIDSDRKLDQEKRKTAQLQKENNGLKKENLDLQKENKTLK
                     SQLNVLMEFAKSQLEKFKEWQKERQQEKENNIARKRDQELER"
     gene            complement(4467..5525)
                     /gene="cfr"
                     /locus_tag="D646_p5002"
                     /db_xref="GeneID:13913676"
     CDS             complement(4467..5525)
                     /gene="cfr"
                     /locus_tag="D646_p5002"
                     /note="mediates the multidrug resistance PhLOPSa
                     resistance phenotype"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA methylase"
                     /protein_id="YP_006958115.1"
                     /db_xref="GI:410655978"
                     /db_xref="GeneID:13913676"
                     /translation="MKEMNFNNKTKYGKIQEFLRSNNEPDYRIKQITNAIFKQRISRF
                     EDMKVLPKLLREDLINNFGETVLNIKLLAEQNSEQVTKVLFEVSKNERVETVNMKYKA
                     GWESFCISSQCGCNFGCKFCATGDIGLKKNLTVDEITDQVLYFHLLGHQIDSISFMGM
                     GEALANRQVFDALDSFTDPNLFALSPRRLSISTIGIIPSIKKITQEYPQVNLTFSLHS
                     PYSEERSKLMPINDRYPIDEVMNILDEHIRLTSRKVYIAYIMLPGVNDSLEHANEVVS
                     LLKSRYKSGKLYHVNLIRYNPTISAPEMYGEANEGQVEAFYKVLKSAGIHVTIRSQFG
                     IDIDAACGQLYGNYQNSQ"
     gene            6068..6931
                     /locus_tag="D646_p5005"
                     /db_xref="GeneID:13914266"
     CDS             6068..6931
                     /locus_tag="D646_p5005"
                     /note="delta pre/mob; essential for plasmid recombination
                     and mobilization"
                     /codon_start=1
                     /transl_table=11
                     /product="recmbination-mobilization protein"
                     /protein_id="YP_006958116.1"
                     /db_xref="GI:410655979"
                     /db_xref="GeneID:13914266"
                     /translation="MQQKIDSLKFRNRFLNVFNRQELLWLQDKFPEHMKKQGFELKRG
                     ERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYSDKVKSDLEVPAKRHMKN
                     VEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDNDKLKQHVRNLMST
                     DMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSLI
                     YESTKEFLKERTDGLKAFKNVFKGFVDKIKDKTAQFQEKHDLEPKKNEFELTHNREVK
                     KERSRDQGMSL"
ORIGIN      
        1 gtgtgattgg ctttataagg ttaatgaacc tttaaaaatc cttgcacaga aaaataccct
       61 atgttattgt ttaagttacc acacaaaaac aaatttacat agggggtttc tgtattaatt
      121 atgtgcaata ataatagcat taattcagat gaaaaatcaa gtgtactaat cgacaaaaca
      181 aaaagtggaa aaataagacc ttggcgagaa aagaaaatgg caaatgtaga ttattttgaa
      241 ctcttgcata ttcttgaatt taaaaaggct gaacgtgtaa aagattgtgc agaggtactt
      301 gaatataaag tgagttatga aacgggagaa aaaacgttat atcgtgtgtg gttttgtaaa
      361 tctcgattat gtccaatgtg caactggagg agagcaatga agcatggtat tcagtcacaa
      421 aaggttgttg cagaagttat caaacaaaaa ccaacagttc gttggctttt ccttacgttg
      481 accgttagaa atgtttatga tggtactgaa ttaaataaaa gtttatcgga tatgtcaaaa
      541 ggttttaatc gtatgatgaa atataagaaa attaataaaa acttagttgg ttttatgcga
      601 gcgacagaag taactgtaaa taatatagat aattcttata accaacacat gcatgtgttg
      661 gtttgtgtag aaagcactta tttttataac actgaaaact atgtgaatca aaagcaatgg
      721 attcaatttt ggaaaagagc tatgaaatta gattatgatc caaacgttaa agtccaaatg
      781 attcgaccaa aaaataaaca taaatctgat ataaaatcag ccattgatga aactgcaaaa
      841 tatccagtaa aagatacaga ttttatgact gatgacgaag aaagaaattt gcagcgttta
      901 gctgatttgg aggaaggact ttatcgaaaa agattaattt catatggtgg tttattaaag
      961 gaaattcata aggaattaaa tcttgatgac gcagaagatg gcgatttaat acatacagat
     1021 gatgaagaaa aagcagatga agatggcttt tctattattg caatgtggaa ttgggaacgg
     1081 aaaaattatt ttattaaaga gtaattcaac aaaagggcca agattgttta agcaaaacaa
     1141 gacaagttaa ggggtgcagt ttacgcaccc tttaatttag tgaaacaatt tgtaactatt
     1201 gaaaatagaa agaaattgtt ccttcgatag tttattaata ttagtgacat ttgcatgctt
     1261 caaagcctgt cggaattggt ttttagtgaa aagaacacga tattcacggt ttacccactt
     1321 ataaacaaaa gatcgatact ttttgtagtc cttctttgaa atcaatggtt gatgtcgttc
     1381 aagaacaatc aatacagagt ctacacttgg cttaggatga aaatatagtg gtggtacttt
     1441 tttgagcatt tttatatcca tctccaccat taatagtaaa cccaaagctc gttgcagatt
     1501 ttgcaatctt ttcgcaaatc ccttctcaac gataagatag ctatatttag cctgactttc
     1561 aaaggtaatt cttttgacaa tatccgtact gatgttataa ggaatattac catatatctt
     1621 atagtttata tgttttggga aggaaaattt tagaatatcc gtttgaatca cttttatatt
     1681 ctcagagggg tttaccgctt ctttagtcac ttgacataag cctccatcaa tttctatagc
     1741 agtaactgat cgactcattt tgactagctc tttggtaaaa tgtccttttc ctgatccgat
     1801 ttctattacg ttgtcttgtt tactgatatt cgtgtgattc aatatttctt ttacatgctt
     1861 tttagaagta ataaaatttt gcgtgtcttt agggtttttc tggttcatta taaccttctc
     1921 cttactggtt ataatgaact gacattaagc gttcatcatt ataaccactt attttttgtt
     1981 tggttgataa tcaactgtgt tgattacaaa aatactaaaa atgcccatat attaattctc
     2041 cttcttataa acttaaaata attatagcac aagctctgat aaataagaac atgatgaacg
     2101 accgttaaat ttatactgca atcgggcgcg attgttgaat aagaatacaa aagatacgag
     2161 agatcattct cgtgtctttt gttattttaa gtggttttgt ccgttatcct ggaaaaccga
     2221 aagtcgagca aattttgatt ttgctgagta cggctttcgg tacgaaaaac ataacggaca
     2281 aaataaaaaa gacaagggtt taaggtggag atttttcagc gcagcgtttt gaaaaatacg
     2341 acctgtccct tgtctttttt aaacgagcca cgagagcaaa tccccctttt gctgaggtgg
     2401 cagaggtcag gtttttttgt ttcttttttc tcgtaaaaaa aagaaaggtc ttaaaggttt
     2461 tgtgcatact ggtaatcagt atgcagggtt ttgttggcag agccaatagc ctcgcagagc
     2521 acacacttta tggatataaa gtatagtgtg ctatacttta catggaagtt gaccgaaata
     2581 agggggcgtg tttttgtgag ttttgctgtg gtacggatgc agaaaatgaa gagtccagat
     2641 ttaaagggca tgcaatttca caaccagcga gaacgggaaa gtcggacgaa tccagacatc
     2701 gatccggacc gtgaacattt gaattatgat ttgctgcatc aagagaaaat tgattacaac
     2761 caacaagtaa aagccatcat cgagagccaa aaagtaagcg aacggaaaac ccgaaaagat
     2821 gccgtgctcg taaatgagct gctggtgaca tcggaccgga aattttttga tgggttagat
     2881 ccggctgaac agaaacggtt tttcgaggaa agccataaat tgttttcgga acgctacggc
     2941 aaacaaaata tcgcttatgc gacggttcac aacgacgaga aaacgcctca catgcattta
     3001 ggggtcgttc cgatgcgtga cggcaaactc cagggcaaga atgtcttcaa tcggcaggag
     3061 ttgcagtgga tgcaggaaga attcccaaaa cacatgcaaa gtgttggttt tgaagtcgaa
     3121 cggggcattg cctctgaccg gaaacacatt gaaatgagcc gttttaaggc gttaacgctc
     3181 aatgaagaga ttaaaaccct tgagaaggaa acagaagccc tcagaaacgc gctgacggct
     3241 tctaagaagg tcgatgagct gcaagtcact aaaccctctc tatttgaccg gaaccacgta
     3301 aaattgcctg tagaggactt tgaagcactt aaagcaagag cgaaggccac ggaagccatc
     3361 gaaaggacca tcgaggctca tgaaaagcga tttgacgata tggtcgataa cgtgatcgac
     3421 agcgatcgga aactggacca agaaaaaaga aaaacggcgc agctgcagaa agaaaataac
     3481 ggtttgaaaa aagaaaatct cgatttgcag aaggaaaaca aaaccctgaa aagccagttg
     3541 aatgttttga tggaatttgc aaaatcccag ctggagaaat tcaaggaatg gcaaaaagag
     3601 cgtcagcaag aaaaagaaaa caatattgct agaaaaagag accaagagtt ggaacgctga
     3661 aaggcgcaga gagtcttcac gcaaagtgcc cagttataca ccaccccaaa tcggggtggt
     3721 gaaaggaatt cacttgtcgc cttcggatgt aaaaaataca taaataaaaa tgaaaaaaca
     3781 gcaataattc gttcgtaaat ttagcttgaa acagaagaac gttcgcaaag tggatggcca
     3841 catttacgaa cgttcttttt ctgttttccg gttgcttttg aggtgttcga aaacgtgtac
     3901 ttttacgaac gctaataaag tgagaaatca aatgatatta aaaggttttc aaaaagttga
     3961 agaagaactt agcaagaggg tttcagatta tataattgag gagggtttta tgaagctgaa
     4021 cgcattcccg aatactttca agttgttctc tagagagact acataaatta actgccaaag
     4081 actacatttt tagaatgcca taaacatctt caagtatata tttcattaac ttacatttgt
     4141 atttagggag aataaaaaat cctcccaatt attattggaa ggattatcta ttcatttata
     4201 ctaaataaga gttttttgtc attcgacaaa caaaacatct agttaaactg atttaaaaaa
     4261 aatggatgac taatcctata tcttagtact ttgaaactta ttcatttcaa attagaagaa
     4321 tacagattag ttaattatct tccacccagt agtccattca tatttgaaga aaatactagc
     4381 ataattgagt tatcaaaata atattaattg cattaatatt atttaaaaat tcaccttatt
     4441 atatcagcta tataccttgt tcaatactat tggctatttt gataattacc atataattga
     4501 ccacaagcag cgtcaatatc aatcccaaat tgacttctaa ttgtgacatg gataccagca
     4561 gacttcaaaa ctttgtaaaa ggcttctacc tgcccttcgt ttgcttctcc atacatctca
     4621 ggtgcactta ttgtaggatt gtatcgtatc aaatttacat gatataactt ccctgattta
     4681 tagcgacttt taagaaggct aacaacttcg tttgcatgct caagagaatc atttacacca
     4741 ggcaacatga tataagctat atatactttc cttgaagtta atcttatatg ttcatcgagt
     4801 atattcatta cctcatctat tgggtatcta tcatttattg gcatcaattt gctgcgttcc
     4861 tcactataag gtgagtgtaa tgaaaatgta agatttactt gaggatattc ctgggttatt
     4921 tttttgatac taggtataat accaatcgtt gatatagaaa gtctacgagg acttaatgca
     4981 aataaattag gatccgtaaa cgaatcaaga gcatcaaata cttgacggtt ggctagagct
     5041 tcacccattc ccataaaaga aatgctatca atttgatgac ctaataaatg gaagtataaa
     5101 acttgatctg ttatctcatc tacagttagg ttttttttca atccaatgtc gcctgtagca
     5161 caaaatttac acccaaaatt acatccgcat tgtgatgata tacaaaatga ctcccaacct
     5221 gctttatact tcatgtttac cgtttctact ctctcattct ttgatacttc aaaaagcact
     5281 ttcgtaactt gctctgaatt ttgctctgct aagagcttga tattcaaaac tgtttctcca
     5341 aaattattta ttaaatcctc cctaagtaat tttggaagaa ccttcatatc ctcaaatcga
     5401 ctaattcttt gtttaaaaat cgcattggtt atttgtttta ttctataatc aggctcatta
     5461 ttacttctta aaaattcctg tattttacca tactttgttt tattattaaa attcatttct
     5521 ttcatccttt cacaatttgc tctagtggta attagaataa ttggaaagga gtagcagtcc
     5581 ctaagaatca atttcttttt gcacacaaaa aaaacacaga taaaaattac cggtgccgaa
     5641 agtcaaaaca tacaaatatg catatttcac tacataaaac aagtatgttg atgatcttgt
     5701 tttaaactta tccatagtag aggcaaagct ccggcaactg tttttatatc attgtttagt
     5761 ttaaaaaagg acagactact ccctttaata gctaaacggt ttccgatttt tgtattttca
     5821 ccgaataaga ttctaaaaac agttaacata gctattacct cctttcacta attaatatac
     5881 ttagcctgta ctgtaacatt ttaatattca aatgtaaata ttaaagttag caacataatg
     5941 taaaacctaa atcaaccaat tttataaaaa cttttagtgc cttgtcttca tctgatattt
     6001 atggactggg gaaaccaact ggacgagata gaaggtgggg gctatttgaa catttacgtt
     6061 aagaaaattg cagcaaaaaa tcgattcctt gaagtttagg aatcgatttt taaatgtgtt
     6121 taatcgtcaa gaactgttat ggttacaaga taaattcccc gagcatatga aaaaacaggg
     6181 ttttgagttg aagcgtggtg aacgtggctc tgaccgtaaa catattgaga cagctaaatt
     6241 taaaaaacaa actttggaaa aagagattga ttttctagaa aaaaacttag cagttaaaaa
     6301 agatgaatgg actgcttata gcgataaagt taaatcagat ttagaagtac cagcgaaacg
     6361 acacatgaaa aatgttgaag tgccaacggg tgaaaagtcc atgtttggtt taggaaaaga
     6421 aataatgaaa acagaaaaga aaccaactaa aaatgttgtt atatcggagc gtgactataa
     6481 aaacttagtg actgctgcga gagataacga taagttaaaa cagcatgtta gaaatctcat
     6541 gagtactgat atggcgagag aatataaaaa attaagtaaa gaacatgggc aagttaaaga
     6601 aaaatatagt ggtcttgtag agcgatttaa cgaaaatgta aatgattata atgagttgct
     6661 tgaagaaaat aagtctttaa agtctaaaat aagcgattta aagcgtgatg tgagtttaat
     6721 ctatgaaagc actaaggaat tccttaagga acgtacagac ggcttaaaag cctttaaaaa
     6781 cgtttttaag gggtttgtag acaagataaa ggataaaaca gcacaattcc aagaaaaaca
     6841 cgatttagaa cctaaaaaga acgaatttga actaactcat aaccgtgagg taaaaaaaga
     6901 acgaagtcga gatcagggaa tgagtttata aaataaaaaa agcacctgaa aaggtgtctt
     6961 tttttgatgg ttttgaactt gttctttctt atcttgatac atatagaaac aacaagtttt
     7021 tatttttata gtttacatcg cgtttaaagt gttg
//
