LOCUS       NC_019149               4809 bp    DNA     circular BCT 03-NOV-2012
DEFINITION  Staphylococcus aureus subsp. aureus plasmid pKKS49, complete
            sequence.
ACCESSION   NC_019149
VERSION     NC_019149.1  GI:410656020
DBLINK      Project: 178791
            BioProject: PRJNA178791
KEYWORDS    complete replicon.
SOURCE      Staphylococcus aureus subsp. aureus
  ORGANISM  Staphylococcus aureus subsp. aureus
            Bacteria; Firmicutes; Bacillales; Staphylococcus.
REFERENCE   1
  AUTHORS   Kadlec,K., Fessler,A.T., Couto,N., Pomba,C.F. and Schwarz,S.
  TITLE     Unusual small plasmids carrying the novel resistance genes dfrK or
            apmA isolated from methicillin-resistant or -susceptible
            staphylococci
  JOURNAL   J. Antimicrob. Chemother. 67 (10), 2342-2345 (2012)
   PUBMED   22718530
REFERENCE   2  (bases 1 to 4809)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2012) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 4809)
  AUTHORS   Schwarz,S.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2011) Schwarz S.P., Institute of Farm Animal
            Genetics (FLI), Molecular Microbiology & Antibiotics, Hoeltystr.
            10, D-31535 Neustadt-Mariensee, GERMANY
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence is identical to HE611647.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Staphylococcus aureus subsp. aureus"
                     /mol_type="genomic DNA"
                     /strain="ST398"
                     /isolate="E49"
                     /isolation_source="Sus scrofa with MRSA"
                     /sub_species="aureus"
                     /db_xref="taxon:46170"
                     /plasmid="pKKS49"
                     /country="Portugal"
     gene            complement(17..667)
                     /gene="rep"
                     /locus_tag="D646_p10001"
                     /db_xref="GeneID:13913712"
     CDS             complement(17..667)
                     /gene="rep"
                     /locus_tag="D646_p10001"
                     /function="involved in plasmid replication"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="YP_006958153.1"
                     /db_xref="GI:410656021"
                     /db_xref="GeneID:13913712"
                     /translation="MSSILITPWSVMIQVVSKMIHIRITACYIFEKGEKVIMAEIHVG
                     AEREVLKPNSMSFEERKIQMEKEEEIEKEIRKRDKNSPYKRWIQLNKDAYKAEDWLMA
                     KSPIAYRIFKFLINNMDSYNAVMCSYKVLEEQFEVSTSTVTRAVKLLKDKQYIDVYKS
                     GTSNVYAVNKQIAWNSWGSNFKHGKFSANVILSESEQDKQTKLKIQRHKEVTLEEI"
     gene            1620..1949
                     /gene="mobC"
                     /locus_tag="D646_p10002"
                     /db_xref="GeneID:13913711"
     CDS             1620..1949
                     /gene="mobC"
                     /locus_tag="D646_p10002"
                     /function="involved in plasmid mobilization"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="YP_006958154.1"
                     /db_xref="GI:410656022"
                     /db_xref="GeneID:13913711"
                     /translation="MSGTRKENRASSRQIKFRVDDSEYERLQQIADTFHMSVPAFAKK
                     RAMGYRMKPPKIDKSGAIEIAKQLRAIGNNVNQLTRRANASTGAIDSEELQAIKKELH
                     AIWQQFS"
     gene            1931..3079
                     /gene="mobA"
                     /locus_tag="D646_p10004"
                     /db_xref="GeneID:13913710"
     CDS             1931..3079
                     /gene="mobA"
                     /locus_tag="D646_p10004"
                     /function="involved in plasmid mobilization"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein A"
                     /protein_id="YP_006958155.1"
                     /db_xref="GI:410656023"
                     /db_xref="GeneID:13913710"
                     /translation="MATIQLGTTRSASRLCNYAEKRAVVKDGHNLDIDYAKSQMKQTR
                     ELFSKNDGIQAHHVIQSFKPNEVTPEKANQVGLELAEKLAKDHEVAVYTHADKDHIHN
                     HIVINSVNFETGKKYQAHGKEAIERARSLSDEVCKNHNLSIVTEHNASVRHTLAEQHL
                     LEKNKPSWKDELREAIEYARNNSTNFDSFKKHLNDVYGVETKLRGKTLSFKHPERERF
                     IRANKLGADYEREGLENVFTRQAKREQEHERTVSRDQGTQRTNEKLYQSSHERGNGER
                     SHDSQSIESNTVEIGRSHEEHAVNLERAREDVERKHRSFAKDFDRWTRGNSKEQQQDH
                     SSTGGTSKDKQRSIGRNERGNQVEREEHAEQSKQRRQKSKTRDDGLSL"
     gene            2634..3182
                     /gene="mobB"
                     /locus_tag="D646_p10003"
                     /db_xref="GeneID:13913708"
     CDS             2634..3182
                     /gene="mobB"
                     /locus_tag="D646_p10003"
                     /function="involved in plasmid mobilization"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein B"
                     /protein_id="YP_006958156.1"
                     /db_xref="GI:410656024"
                     /db_xref="GeneID:13913708"
                     /translation="MSLQDKLNENRSMSELFQETRELNELMKSYIRVHMNEEMAKEVT
                     IHNQLNQIQSKSDEVMRNMQSILNEQEKMLKENTEVLQKILIDGHEEIQRNNNRTIQA
                     LGELQKTNNAQLEEMNEEIKSSVRNMQNNLNSVVKKARQETMDLVSSAKHGALVTNII
                     DALKYGAATSVITVPILYLLLS"
     gene            3682..4506
                     /gene="apmA"
                     /locus_tag="D646_p10005"
                     /db_xref="GeneID:13913709"
     CDS             3682..4506
                     /gene="apmA"
                     /locus_tag="D646_p10005"
                     /function="confers apramycin resistance"
                     /codon_start=1
                     /transl_table=11
                     /product="aminocyclitol acetyltransferase"
                     /protein_id="YP_006958157.1"
                     /db_xref="GI:410656025"
                     /db_xref="GeneID:13913709"
                     /translation="MKTRLEQVLERYLNGREVVVWGVPTRRLLRALKPFKFRVDDRVD
                     PQYHYVVAVNDDDLSDFLSDEQSKSFQYANDYLTFDDEGGELPFERMCFNVPVGRQTY
                     FGDGVVGACKNGYIKSIGQFTSINGTAEIHVNHQLNMTFVSDDIQNFFNEESMAIFHE
                     KLRKDPKHPYAYSKEPMTIGSDVYIGAHAFINASTVTSIGDGAIIGSGAVVLENVPPF
                     AVVVGVPARIKRYRFSKEMIETLLRVKWWDWSIEEINENADALISPELFMKKYGGL"
ORIGIN      
        1 ttgatttaac gtgatgttaa atttcttcta aagtaacctc tttatgtctt tgaattttta
       61 atttagtttg tttatcttgt tcgctttcag aaagaataac attcgcacta aatttaccat
      121 gcttgaaatt acttccccaa ctgttccaag caatttgttt attaactgca tatacgtttg
      181 atgtacctga tttatataca tcaatgtatt gcttatcttt aagcaattta acggctcttg
      241 ttaccgttga agtacttact tcgaattgtt cctctaagac tttataagag cacattacag
      301 cgttgtatga atccatatta tttattaaga atttaaaaat tctatatgct attggtgatt
      361 ttgccattag ccaatcttct gctttataag cgtctttatt taactgtatc cacctcttgt
      421 aaggactgtt tttatctctc ttgcgaattt ccttttcgat ttcttcttcc ttctccattt
      481 ggatcttacg ctcttcaaaa ctcatagaat taggttttag aacttctctt tctgctccta
      541 catgtatttc agccattatt actttctccc ctttttcgaa tatgtaacac gctgtgatac
      601 gtatatgtat catttttgat acaacttgta tcattactga ccatggtgtg attagtatac
      661 tgctcattta tggttattct atcgtcatta atgaccattc tatgatactg tttgactaaa
      721 gtcaagtctt atagtgacta tctagtgctc taatactagt cacaccatga ctagtatagt
      781 aatcacacca tgactagtaa aaaacgctct atccattgat atgacaacaa ctagcttata
      841 ttcactctta tatcttctat aaggcacttt tctacatgca agggtcgggt agtatttttt
      901 aggaaaacca cctaaaaaat ctccacgcct ttaccccttg cattccggaa tttttcggct
      961 gtctaggctt cgccgaacga ccgatatacg aaatagggat attgagtatt cgctcgttcg
     1021 cttgtccagt gtagccgaaa aaccgttaag agtgtcggcg ataatgtgat tctttcgcta
     1081 tgtatagaat tcgttatgcg ggggctagcc cccacacccc cagcctcact ccctcaaccg
     1141 aaataggggg gaaagaccaa gttcaaaggc attgcgaaat atggttaaaa gctttctgac
     1201 tggaagaaag ctaaatatgg tatgtgtcaa tggtaaatcg caacggaatg ggtcagcggt
     1261 ttttgctgat ccatgaagtg agaatttaag gaattgacac attcgcaaaa gtaccctctt
     1321 ggcttgtcga aaaaccattt tttcgacctg cctaccggcg agggagcccc cgaagattcg
     1381 ggggttgggg gcttgatatg tgaaagtttt tggggttgcc ttttttcaaa aaggcaaggt
     1441 cttaatcccc ccatgtaaat gggggttagg gggcattttt gcgacagcaa aaacaagctt
     1501 atttgggtac caaatataga gcttgccaat cctcacaata caagttacaa tgagagtgaa
     1561 tagcgagcat ttaatttgta tgatatgcga ttgaaaaatg agcatagtga ggtggttgta
     1621 tgtcgggaac gaggaaagaa aatcgggcga gtagtcggca gattaaattt cgagtggacg
     1681 acagcgaata tgaaaggtta cagcaaattg ctgatacgtt tcatatgagc gtgccagctt
     1741 ttgcaaaaaa gcgggcaatg ggctatcgaa tgaagccacc gaaaattgat aaatcggggg
     1801 caattgagat agcaaagcag ttgcgagcaa ttgggaacaa cgtgaatcaa ttgactaggc
     1861 gagcaaatgc aagcactggg gcgattgata gcgaagaatt acaagctatc aaaaaggagt
     1921 tgcatgcaat atggcaacaa ttcagctagg tacaactcgt tctgctagtc gattatgcaa
     1981 ctatgcagaa aaaagggcag ttgtgaaaga tggccataat cttgatattg attatgcaaa
     2041 gtcacaaatg aagcaaacaa gggaactttt ttctaaaaat gatggaatac aagcacatca
     2101 tgttatccag tcattcaaac ctaacgaggt tacaccagag aaagcgaatc aagtaggttt
     2161 agaattagct gagaaattag ctaaagacca tgaagtagct gtttacacac atgcagataa
     2221 agaccatatt cataatcata tcgtgataaa ttcagttaac tttgaaactg gtaaaaagta
     2281 tcaagcacat ggaaaagaag caatcgagcg tgctagatca ttgagcgatg aagtatgcaa
     2341 aaatcataat ttatcaatag ttacagagca taacgctagt gttcgtcata ctttggcaga
     2401 acaacattta ctagaaaaaa ataaaccatc ttggaaagat gaattgcgtg aagcgattga
     2461 gtacgctcga aataattcaa caaactttga tagctttaaa aagcatttaa atgacgttta
     2521 cggggtcgaa acgaaattga ggggtaaaac acttagtttt aaacatccag aacgagaacg
     2581 ctttatacgg gctaataaat tgggtgctga ctatgaaagg gaggggctag aaaatgtctt
     2641 tacaagacaa gctaaacgag aacaggagca tgagcgaact gtttcaagag accagggaac
     2701 tcaacgaact aatgaaaagt tatatcagag ttcacatgaa cgaggaaatg gcgaaagaag
     2761 tcacgattca caatcaattg aatcaaatac agtcgaaatc ggacgaagtc atgaggaaca
     2821 tgcagtcaat cttgaacgag caagagaaga tgttgaaaga aaacaccgaa gttttgcaaa
     2881 agattttgat agatggacac gaggaaattc aaaggaacaa caacaggacc attcaagcac
     2941 tgggggaact tcaaaagaca aacaacgctc aattggaaga aatgaacgag gaaatcaagt
     3001 cgagcgtgag gaacatgcag aacaatctaa acagcgtcgt caaaaaagca agacaagaga
     3061 cgatggactt agtctctagt gcaaagcatg gggcattggt tacgaatatc attgatgctt
     3121 taaaatatgg tgcagctact agcgtgatca ctgtaccaat cttatatctt ttactttcct
     3181 agcaaaaaga ggttggcttt atgccaaccc ctaagcctta acggcaatgg ttttctctat
     3241 cgaataatat gtctgttgct ttttctagta aactcatgcc atatagagga tggccaactt
     3301 gtgatcagtt gtgaatggca aaaggtttct ctgtatagac aaacaaatcc cgagtggttg
     3361 aaacaaagca cacaaactgt tcagtttaac gttgaaggag tgatacaagc aatctggaag
     3421 gtgctccgcg gtaaacagcg tgagatggca aatggtggat ttgaaaaaga caggggaacg
     3481 ctcaaaaagt tgatagataa attgacttgt gtattaataa ttgatgagga aaataaaaaa
     3541 gggacaaaga aactcctttt agatccatat gtgtagatgt attaaaacgt gaaatgaaag
     3601 ttacctgttt aataactgat aaatagtaat ttgtcgggca gttttgaact ggggattaca
     3661 tattttggga ggtagatact tatgaaaacc agacttgaac aagttttaga acgctatctc
     3721 aacggacgcg aggtagttgt atggggtgtt ccgacacgcc gtttgcttcg tgcattaaaa
     3781 ccttttaaat ttcgtgttga cgatcgcgtt gaccctcaat atcattatgt tgttgctgta
     3841 aacgatgacg acttatccga ctttttatcc gatgaacaga gcaagtcgtt tcaatacgct
     3901 aatgactatc tgacgtttga cgatgaggga ggcgaacttc cgtttgaacg aatgtgcttc
     3961 aatgtccccg ttggcaggca aacgtatttt ggagacggtg tcgtaggagc ttgcaaaaac
     4021 gggtatatca agagcatcgg ccaatttaca tcgattaacg gcacggcaga gatccatgtg
     4081 aaccatcagt taaacatgac ctttgtaagc gacgatattc aaaacttctt caatgaagag
     4141 agtatggcta tattccatga aaagctgcga aaggacccga aacaccctta tgcttacagc
     4201 aaagaaccta tgaccatagg tagcgatgtt tatatcggcg cacatgcatt catcaatgct
     4261 tccactgtga caagcatcgg tgacggtgct ataatcggct cgggcgcagt ggttctggaa
     4321 aatgttcctc cttttgctgt cgtcgttggt gtccctgcga gaatcaagcg ttaccgcttc
     4381 tcaaaggaga tgattgaaac cctgcttcgt gtcaaatggt gggactggag tatagaggaa
     4441 attaatgaaa atgctgatgc gcttatatca cctgaactat ttatgaagaa gtacgggggt
     4501 ttatagcgta tatgcgagta atacgaaatt tgtatatcgt gagcataaca cgaagccgac
     4561 aaattccaaa aagcaagaca agagacgatg gacttagtct ctagtgcaaa gcatggggca
     4621 ttggttacga atatcattga tgctttaaaa tatggtgttg ctacaagtgc aattactgtt
     4681 ccaattctct atttgctcct tagttaatta aaaagactgg ctttatgcca gtcctttctg
     4741 ctttagctct agagggttgt aagctatgtt atccaatatt gtcaagtctg tacaacctgt
     4801 atgttatat
//
