LOCUS       NC_019139               2402 bp    DNA     circular BCT 03-NOV-2012
DEFINITION  Staphylococcus aureus plasmid pDLK1, complete sequence.
ACCESSION   NC_019139
VERSION     NC_019139.1  GI:410655957
DBLINK      Project: 178791
            BioProject: PRJNA178791
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcus.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Kuntova,L., Pantucek,R., Rajova,J., Ruzickova,V., Petras,P.,
            Maslanova,I. and Doskar,J.
  TITLE     Characteristics and distribution of plasmids in a clonally diverse
            set of methicillin-resistant Staphylococcus aureus strains
  JOURNAL   Arch. Microbiol. 194 (7), 607-614 (2012)
   PUBMED   22331232
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Kuntova,L., Kahankova,J., Pantucek,R., Ruzickova,V., Petras,P.,
            Nepejchalova,I. and Doskar,J.
  TITLE     Characteristics and diversity of resistance plasmids in hospital-
            and community-acquired methicillin-resistant Staphylococcus aureus
            strains in the Czech Republic
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 2402)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2012) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   4  (bases 1 to 2402)
  AUTHORS   Kuntova,L. and Doskar,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2010) Department of Experimental Biology, Masaryk
            University, Kotlarska 2, Brno 61137, Czech Republic
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence is identical to GU562624.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="E14"
                     /isolation_source="clinical isolate"
                     /db_xref="taxon:1280"
                     /plasmid="pDLK1"
                     /note="methicillin-resistant strain"
     gene            complement(28..504)
                     /gene="repL"
                     /locus_tag="D646_p1"
                     /db_xref="GeneID:13913655"
     CDS             complement(28..504)
                     /gene="repL"
                     /locus_tag="D646_p1"
                     /note="RepL"
                     /codon_start=1
                     /transl_table=11
                     /product="firmicute plasmid replication protein"
                     /protein_id="YP_006958100.1"
                     /db_xref="GI:410655959"
                     /db_xref="GeneID:13913655"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIV
                     QLISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEE
                     GNIIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
     misc_feature    complement(154..339)
                     /gene="repL"
                     /locus_tag="D646_p1"
                     /note="helix_turn_helix, cAMP Regulatory protein
                     C-terminus; DNA binding domain of prokaryotic regulatory
                     proteins belonging to the catabolite activator protein
                     family; Region: HTH_CRP; cd00092"
                     /db_xref="CDD:28976"
     misc_feature    complement(325..330)
                     /gene="repL"
                     /locus_tag="D646_p1"
                     /note="putative switch regulator; other site"
                     /db_xref="CDD:28976"
     misc_feature    complement(order(238..240,265..273,277..279))
                     /gene="repL"
                     /locus_tag="D646_p1"
                     /note="non-specific DNA interactions [nucleotide binding];
                     other site"
                     /db_xref="CDD:28976"
     misc_feature    complement(220..240)
                     /gene="repL"
                     /locus_tag="D646_p1"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:28976"
     misc_feature    complement(order(220..222,232..237))
                     /gene="repL"
                     /locus_tag="D646_p1"
                     /note="sequence specific DNA binding site [nucleotide
                     binding]; other site"
                     /db_xref="CDD:28976"
     misc_feature    complement(232..237)
                     /gene="repL"
                     /locus_tag="D646_p1"
                     /note="putative cAMP binding site [chemical binding];
                     other site"
                     /db_xref="CDD:28976"
     gene            complement(952..1686)
                     /gene="ermC"
                     /locus_tag="D646_p2"
                     /db_xref="GeneID:13913654"
     CDS             complement(952..1686)
                     /gene="ermC"
                     /locus_tag="D646_p2"
                     /EC_number="2.1.1.-"
                     /note="23S RNA methylase"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyladenosine transferase"
                     /protein_id="YP_006958101.1"
                     /db_xref="GI:410655958"
                     /db_xref="GeneID:13913654"
                     /translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHF
                     TLELVQRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIFGNI
                     PYNISTDIIRKIVFDSIADEIYLIVEYGFAKRLLNTKRSLALFLMAEVDISILSMVPR
                     EYFHPKPKVNSSLIRLNRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKH
                     AGIDDLNNISFEQFLSLFNSYKLFNK"
     misc_feature    complement(967..1659)
                     /gene="ermC"
                     /locus_tag="D646_p2"
                     /note="16S ribosomal RNA methyltransferase KsgA/Dim1
                     family protein; Reviewed; Region: ksgA; PRK00274"
                     /db_xref="CDD:178953"
     misc_feature    complement(1342..1590)
                     /gene="ermC"
                     /locus_tag="D646_p2"
                     /note="S-adenosylmethionine-dependent methyltransferases
                     (SAM or AdoMet-MTase), class I;  AdoMet-MTases are enzymes
                     that use S-adenosyl-L-methionine (SAM or AdoMet) as a
                     substrate for methyltransfer, creating the product
                     S-adenosyl-L-homocysteine (AdoHcy); Region: AdoMet_MTases;
                     cd02440"
                     /db_xref="CDD:100107"
     misc_feature    complement(order(1384..1386,1432..1440,1507..1512,
                     1558..1578))
                     /gene="ermC"
                     /locus_tag="D646_p2"
                     /note="S-adenosylmethionine binding site [chemical
                     binding]; other site"
                     /db_xref="CDD:100107"
ORIGIN      
        1 tctatttgta gacgatttta tgttatacta gtccttgaaa gaataataat cagataatgc
       61 attttcttgt ttttcatttg cctcttgctc aaagttccca aattcgagta agaggtattt
      121 ttgtttttgg tcgtcgcctc tcattagtag ttcagggttt aacattaata ctccagtttt
      181 tctttttata atatttcctt cttctaagat tttaagtgtt gttattactg tttgtagact
      241 tgttcctgta gcttttgcta tttctcttgt tgtagctatc attgtattgt tacttaagtg
      301 gacattatct aggatatagt taacgatttt aagttttttt ccgccaatca tatctaacat
      361 acttattaat tgcactatat atgcctttac gaagttacca gacgtttgtt tacggtataa
      421 cttgtctacc tctatgactt ctccactttc ttcgtctatg agcctctgag agcctttata
      481 gactgttcca tatctttctt tcatcttttt ctcactcctt attttaaact attctaacta
      541 tatcataact gttctaaaaa aaaaagaaca tttgttaaaa gaaattagaa caaaatgagt
      601 gaaaaattag aacaaacaaa ttccttataa accttatcat ctcaacctat attaagattt
      661 tacctagttg aatcttcttt tctatataaa gcgtcggagc atatcagggg gttatctaac
      721 gtaaatgcta cccttcggct cgctttcgct cggcattgac gtcagatact gcaccccctg
      781 aacccccatg ctccaacagc aaaaaggaaa ctttttgctg cttttccgac gcttattcgc
      841 ttcgctcata tttatataga aaagaagtga atgcgcaaaa gacataatcg attcacaaaa
      901 aataggtaca cgaaaaacaa gttaagggat gcataaactg catcccttaa cttacttatt
      961 aaataattta tagctattga aaagagataa gaattgttca aagctaatat tgtttaaatc
     1021 gtcaattcct gcatgtttta aggaattgtt aaattgattt tttgtaaata ttttcttgta
     1081 ttctttgtta acccatttca taacgaaata attatacttc tgtttatctt tgtgtgatat
     1141 tcttgatttt tttctattta atctgataag tgagctattc actttaggtt taggatgaaa
     1201 atattctctt ggaaccatac ttaatataga aatatcaact tctgccatta aaaataatgc
     1261 caatgagcgt tttgtattta ataatctttt agcaaacccg tattccacga ttaaataaat
     1321 ctcatcagct atactatcaa aaacaatttt gcgtattata tccgtactta tgttataagg
     1381 tatattacca aatattttat aggattggtt tttaggaaat ttaaactgca atatatcctt
     1441 gtttaaaact tggaaattat cgtgatcaac aagtttattt tctgtagttt tgcataattt
     1501 atggtctatt tcaatggcag ttacgaaatt acacctctgt actaattcaa gggtaaaatg
     1561 cccttttcct gagccgattt caaagatatt atcatgttca tttaatctta tatttgtcat
     1621 tattttatct atattatgtt ttgaagtaat aaagttttga ctgtgtttta tatttttctc
     1681 gttcattata accctcttta atttggttat aatgaatttt gcttattaac gattcattat
     1741 aaccacttaa taaaattagt ataattatag cacgagctct gataaatatg aacatgatga
     1801 gtgatcgtta aatttatact gcaatctgat gcgattattg aataaaagat atgagagatt
     1861 tatctagttt ctttttttac aagaaaaaag aaagttctta aaggttttat acttttggtc
     1921 gtagagcaca cggtttaacg acttaattac gaagtaaata agtctagtgt gttagacttt
     1981 aatgtttttt taaggcatta gtgcatttaa gcgtcagagc atggctttat gccgagaaaa
     2041 ctattggttg gaatggcgtg tgtgttagcc aaagctttgg cgagttggtt gggggtttca
     2101 tgggattaat cccatgaaag taccaactca acaacacact aacgcctgtt ggttccaacc
     2161 aataggaaat tggaataagc aattagtata atgagagtat aatgttggta taacgttagt
     2221 ataatgatgc tttttttcat tatatttttt atgtacttta aacctgcacg cttatgtgaa
     2281 ttagaaaaag cttaatcgca tttcatagat tgacctccca ataactacgt ggtgttattg
     2341 ggaggtcaat ctatttcatt tgcgttaagc ttttgatcta cattaagcgt gcaggttttt
     2401 tg
//
