LOCUS       NC_017264               9600 bp    DNA     circular CON 08-JUN-2013
DEFINITION  Yersinia pestis biovar Medievalis str. Harbin 35 plasmid pPCP,
            complete sequence.
ACCESSION   NC_017264
VERSION     NC_017264.1  GI:384412693
DBLINK      Project: 158537
            BioProject: PRJNA158537
KEYWORDS    .
SOURCE      Yersinia pestis biovar Medievalis str. Harbin 35
  ORGANISM  Yersinia pestis biovar Medievalis str. Harbin 35
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Yersinia.
REFERENCE   1  (bases 1 to 9600)
  AUTHORS   Plunkett,G. III, Anderson,B.D., Baumler,D.J., Burland,V.,
            Cabot,E.L., Glasner,J.D., Mau,B., Neeno-Eckwall,E., Perna,N.T.,
            Munk,A.C., Han,C.S., Bruce,D.C., Detter,J.C., Tapia,R.,
            Brettin,T.S., Mane,S.P. and Sobral,B.W.
  TITLE     Yersinia pestis Harbin 35, complete genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9600)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2012) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 9600)
  AUTHORS   Plunkett,G. III, Anderson,B.D., Baumler,D.J., Burland,V.,
            Cabot,E.L., Glasner,J.D., Mau,B., Neeno-Eckwall,E., Perna,N.T.,
            Munk,A.C., Han,C.S., Bruce,D.C., Detter,J.C., Tapia,R.,
            Brettin,T.S., Mane,S.P. and Sobral,B.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2011) The Pathosystems Resource Integration
            Center (PATRIC), Virginia Bioinformatics Institute at Virginia
            Polytechnic Institute and State University, Bioinformatics I,
            Washington Street, Blacksburg, VA 24061, USA
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence is identical to CP001611.
            This strain is a human isolate from China and is being sequenced
            for a comparative genome analysis of Yersinia pestis strains from
            different parts of the world.
             The genome was sequenced using a combination of 3 kb and 8 kb DNA
            libraries.  All general aspects of library construction and
            sequencing performed at the JGI can be found at
            http://www.jgi.doe.gov/. Draft assemblies were based on 79539 total
            reads.  The two libraries provided 16x coverage of the genome.  The
            Phred/Phrap/Consed software package (www.phrap.com) was used for
            sequence assembly and quality assessment.  After the shotgun stage,
            reads were assembled with parallel phrap (High Performance
            Software, LLC).  Possible mis-assemblies were corrected with
            Dupfinisher and PCR.  Gaps between contigs were closed by editing
            in Consed, custom primer walk or PCR amplification.  Approximately
            360 additional custom primer walk reactions were necessary to close
            gaps and to raise the quality of the finished sequence.  The
            completed genome sequences contain 80176 reads, achieving an
            average of 16-fold sequence coverage per base with an error rate
            less than 1 in 100,000.
             Genome sequencing, assembly, and finishing were performed by the
            Department of Energy's Joint Genome Institute (DOE-JGI), in Walnut
            Creek, CA and at Los Alamos National Laboratory, Los Alamos, NM.
            Annotation was carried out by the Enteropathogen Resource
            Integration Center, Madison, WI (ERIC
            http://www.ericbrc.org/portal/eric), a Bioinformatics Resource
            Center supported by the NIH/NIAID.The Pathosystems Resource
            Integration Center  (PATRIC http://www.patricbrc.org), Virginia
            Bioinformatics Institute at  Virginia Polytechnic Institute and
            State University, Bioinformatics I,  Washington Street, Blacksburg,
            VA 24061, USA
             Acknowledgements: Intelligence  Technology Innovation Center for
            funding the sequencing, and the NIH/NIAID  for funding the
            annotation.
            DNA/bacteria source is David Bruce (dbruce@lanl.gov).
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9600
                     /organism="Yersinia pestis biovar Medievalis str. Harbin
                     35"
                     /mol_type="genomic DNA"
                     /strain="Harbin 35"
                     /host="Homo sapiens"
                     /db_xref="taxon:547048"
                     /plasmid="pPCP"
                     /country="China"
                     /biovar="Medievalis"
     gene            87..1031
                     /locus_tag="YPC_4879"
                     /db_xref="GeneID:12089379"
     CDS             87..1031
                     /locus_tag="YPC_4879"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="YP_005622057.1"
                     /db_xref="GI:384412694"
                     /db_xref="ASAP:BBE-0004839"
                     /db_xref="ASAP:BBE-0004839 ERIC"
                     /db_xref="GeneID:12089379"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVA
                     NQRKSRPVPAIAGSKSSSPCWHCLRRKKSMTCILMKIW"
     misc_feature    87..923
                     /locus_tag="YPC_4879"
                     /note="Transposase and inactivated derivatives [DNA
                     replication, recombination, and repair]; Region: COG4584"
                     /db_xref="CDD:226950"
     misc_feature    93..200
                     /locus_tag="YPC_4879"
                     /note="Helix-turn-helix domain of Hin and related
                     proteins, a family of DNA-binding domains unique to
                     bacteria and represented by the Hin protein of Salmonella.
                     The basic HTH domain is a simple fold comprised of three
                     core helices that form a right-handed...; Region:
                     HTH_Hin_like; cd00569"
                     /db_xref="CDD:119388"
     misc_feature    order(180..185,189..197)
                     /locus_tag="YPC_4879"
                     /note="DNA-binding interface [nucleotide binding]; DNA
                     binding site"
                     /db_xref="CDD:119388"
     misc_feature    444..812
                     /locus_tag="YPC_4879"
                     /note="Integrase core domain; Region: rve; pfam00665"
                     /db_xref="CDD:216050"
     gene            971..1099
                     /locus_tag="YPC_4880"
                     /db_xref="GeneID:12089380"
     CDS             971..1099
                     /locus_tag="YPC_4880"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="YP_005622058.1"
                     /db_xref="GI:384412695"
                     /db_xref="ASAP:BBE-0004840"
                     /db_xref="ASAP:BBE-0004840 ERIC"
                     /db_xref="GeneID:12089380"
                     /translation="MLALPPEKKEYDVHLDENLVNFDKHPLHHPLSIYDSFCRGVA"
     gene            1096..1878
                     /locus_tag="YPC_4881"
                     /db_xref="GeneID:12089381"
     CDS             1096..1878
                     /locus_tag="YPC_4881"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="YP_005622059.1"
                     /db_xref="GI:384412696"
                     /db_xref="ASAP:BBE-0004841"
                     /db_xref="ASAP:BBE-0004841 ERIC"
                     /db_xref="GeneID:12089381"
                     /translation="MMMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFL
                     EHLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIER
                     NENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQ
                     RGVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGD
                     AALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
     misc_feature    1099..1875
                     /locus_tag="YPC_4881"
                     /note="transposase/IS protein; Provisional; Region:
                     PRK09183"
                     /db_xref="CDD:181681"
     misc_feature    1357..>1659
                     /locus_tag="YPC_4881"
                     /note="The AAA+ (ATPases Associated with a wide variety of
                     cellular Activities) superfamily represents an ancient
                     group of ATPases belonging to the ASCE (for additional
                     strand, catalytic E) division of the P-loop NTPase fold.
                     The ASCE division also includes ABC; Region: AAA; cd00009"
                     /db_xref="CDD:99707"
     misc_feature    1423..1446
                     /locus_tag="YPC_4881"
                     /note="Walker A motif; other site"
                     /db_xref="CDD:99707"
     misc_feature    order(1426..1449,1609..1611)
                     /locus_tag="YPC_4881"
                     /note="ATP binding site [chemical binding]; other site"
                     /db_xref="CDD:99707"
     misc_feature    1597..1614
                     /locus_tag="YPC_4881"
                     /note="Walker B motif; other site"
                     /db_xref="CDD:99707"
     gene            2915..3109
                     /gene="rop"
                     /locus_tag="YPC_4882"
                     /db_xref="GeneID:12089382"
     CDS             2915..3109
                     /gene="rop"
                     /locus_tag="YPC_4882"
                     /codon_start=1
                     /transl_table=11
                     /product="putative replication regulatory protein"
                     /protein_id="YP_005622060.1"
                     /db_xref="GI:384412697"
                     /db_xref="ASAP:BBE-0004842"
                     /db_xref="ASAP:BBE-0004842 ERIC"
                     /db_xref="GeneID:12089382"
                     /translation="MNKQQQTALNMARFIRSQSLILLEKLDALDADEQAAMCERLHEL
                     AEELQNSIQARFEAESETGT"
     misc_feature    2915..3097
                     /gene="rop"
                     /locus_tag="YPC_4882"
                     /note="Rop protein; Region: Rop; pfam01815"
                     /db_xref="CDD:145136"
     gene            complement(3450..3572)
                     /locus_tag="YPC_4883"
                     /db_xref="GeneID:12089383"
     CDS             complement(3450..3572)
                     /locus_tag="YPC_4883"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_005622061.1"
                     /db_xref="GI:384412698"
                     /db_xref="ASAP:BBE-0004843"
                     /db_xref="ASAP:BBE-0004843 ERIC"
                     /db_xref="GeneID:12089383"
                     /translation="MSVDFCDARQGGGAYGKSASGAAFSGVFVFCSHVLSGVIP"
     gene            4345..4770
                     /gene="pim"
                     /locus_tag="YPC_4884"
                     /db_xref="GeneID:12089384"
     CDS             4345..4770
                     /gene="pim"
                     /locus_tag="YPC_4884"
                     /codon_start=1
                     /transl_table=11
                     /product="pesticin immunity protein"
                     /protein_id="YP_005622062.1"
                     /db_xref="GI:384412699"
                     /db_xref="ASAP:BBE-0004844"
                     /db_xref="ASAP:BBE-0004844 ERIC"
                     /db_xref="GeneID:12089384"
                     /translation="MISKLFCLALIFLSSSGLAEKNTYTAKDILQNLELNTFGNSLSH
                     GIYGKQTTFKQTEFTNIKSNTKKHIALINKDNSWMISLKILGIKRDEYTVCFEDFSLI
                     RPPTYVAIHPLLIKKVKSGNFIVVKEIKKSIPGCTVYYH"
     gene            complement(4805..5878)
                     /gene="pst"
                     /locus_tag="YPC_4885"
                     /db_xref="GeneID:12089385"
     CDS             complement(4805..5878)
                     /gene="pst"
                     /locus_tag="YPC_4885"
                     /codon_start=1
                     /transl_table=11
                     /product="pesticin"
                     /protein_id="YP_005622063.1"
                     /db_xref="GI:384412700"
                     /db_xref="ASAP:BBE-0004845"
                     /db_xref="ASAP:BBE-0004845 ERIC"
                     /db_xref="GeneID:12089385"
                     /translation="MSDTMVVNGSGGVPAFLFSGSTLSSYRPNFEANSITIALPHYVD
                     LPGRSNFKLMYIMGFPIDTEMEKDSEYSNKIRQESKISKTEGTVSYEQKITVETGQEK
                     DGVKVYRVMVLEGTIAESIEHLDKKENEDILNNNRNRIVLADNTVINFDNISQLKEFL
                     RRSVNIVDHDIFSSNGFEGFNPTSHFPSNPSSDYFNSTGVTFGSGVDLGQRSKQDLLN
                     DGVPQYIADRLDGYYMLRGKEAYDKVRTAPLTLSDNEAHLLSNIYIDKFSHKIEGLFN
                     DANIGLRFSDLPLRTRTALVSIGYQKGFKLSRTAPTVWNKVIAKDWNGLVNAFNNIVD
                     GMSDRRKREGALVQKDIDSGLLK"
     misc_feature    complement(5381..5878)
                     /gene="pst"
                     /locus_tag="YPC_4885"
                     /note="Pesticin Translocation And Receptor Binding Domain;
                     Region: Pesticin_RB; cd12220"
                     /db_xref="CDD:240617"
     misc_feature    complement(5855..5878)
                     /gene="pst"
                     /locus_tag="YPC_4885"
                     /note="TonB box [polypeptide binding]; other site"
                     /db_xref="CDD:240617"
     gene            5996..6412
                     /locus_tag="YPC_4886"
                     /db_xref="GeneID:12089386"
     CDS             5996..6412
                     /locus_tag="YPC_4886"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_005622064.1"
                     /db_xref="GI:384412701"
                     /db_xref="ASAP:BBE-0004846"
                     /db_xref="ASAP:BBE-0004846 ERIC"
                     /db_xref="GeneID:12089386"
                     /translation="MKFHFCDLNHSYKNQEGKIRSRKTAPGNIRKKQKGDNVSKTKSG
                     RHRLSKTDKRLLAALVVAGYEERTARDLIQKHVYTLTQADLRHLVSEISNGVGQSQAY
                     DAIYQARRIRLARKYLSGKKPEGVEPREGQEREDLP"
     gene            6655..7593
                     /gene="pla"
                     /locus_tag="YPC_4887"
                     /db_xref="GeneID:12089387"
     CDS             6655..7593
                     /gene="pla"
                     /locus_tag="YPC_4887"
                     /EC_number="3.4.23.48"
                     /codon_start=1
                     /transl_table=11
                     /product="coagulase/fibrinolysin precursor"
                     /protein_id="YP_005622065.1"
                     /db_xref="GI:384412702"
                     /db_xref="ASAP:BBE-0004847"
                     /db_xref="ASAP:BBE-0004847 ERIC"
                     /db_xref="GeneID:12089387"
                     /translation="MKKSSIVATIITILSGSANAASSQLIPNISPDSFTVAASTGMLS
                     GKSHEMLYDAETGRKISQLDWKIKNVAILKGDISWDPYSFLTLNARGWTSLASGSGNM
                     DDYDWMNENQSEWTDHSSHPATNVNHANEYDLNVKGWLLQDENYKAGITAGYQETRFS
                     WTATGGSYSYNNGAYTGNFPKGVRVIGYNQRFSMPYIGLAGQYRINDFELNALFKFSD
                     WVRAHDNDEHYMRDLTFREKTSGSRYYGTVINAGYYVTPNAKVFAEFTYSKYDEGKGG
                     TQTIDKNSGDSVSIGGDAAGISNKNYTVTAGLQYRF"
     misc_feature    6655..7590
                     /gene="pla"
                     /locus_tag="YPC_4887"
                     /note="outer membrane protease; Reviewed; Region:
                     PRK12580"
                     /db_xref="CDD:79055"
     gene            complement(7780..8079)
                     /locus_tag="YPC_4888"
                     /db_xref="GeneID:12089388"
     CDS             complement(7780..8079)
                     /locus_tag="YPC_4888"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transcriptional regulator"
                     /protein_id="YP_005622066.1"
                     /db_xref="GI:384412703"
                     /db_xref="ASAP:BBE-0004848"
                     /db_xref="ASAP:BBE-0004848 ERIC"
                     /db_xref="GeneID:12089388"
                     /translation="MRTLDEVIASRSPESQTRIKEMADEMILEVGLQMMREELQLSQK
                     QVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMGGKLSLDVELPTGRRVAFHV"
     misc_feature    complement(7840..7986)
                     /locus_tag="YPC_4888"
                     /note="Helix-turn-helix XRE-family like proteins.
                     Prokaryotic DNA binding proteins belonging to the
                     xenobiotic response element family of transcriptional
                     regulators; Region: HTH_XRE; cd00093"
                     /db_xref="CDD:238045"
     misc_feature    complement(order(7894..7896,7969..7971,7981..7983))
                     /locus_tag="YPC_4888"
                     /note="non-specific DNA binding site [nucleotide binding];
                     other site"
                     /db_xref="CDD:238045"
     misc_feature    complement(order(7897..7899,7972..7974))
                     /locus_tag="YPC_4888"
                     /note="salt bridge; other site"
                     /db_xref="CDD:238045"
     misc_feature    complement(order(7891..7896,7906..7908,7915..7917,
                     7948..7953))
                     /locus_tag="YPC_4888"
                     /note="sequence-specific DNA binding site [nucleotide
                     binding]; other site"
                     /db_xref="CDD:238045"
     gene            complement(8079..8426)
                     /locus_tag="YPC_4889"
                     /db_xref="GeneID:12089389"
     CDS             complement(8079..8426)
                     /locus_tag="YPC_4889"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_005622067.1"
                     /db_xref="GI:384412704"
                     /db_xref="ASAP:BBE-0004849"
                     /db_xref="ASAP:BBE-0004849 ERIC"
                     /db_xref="GeneID:12089389"
                     /translation="MWMVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRPYA
                     DTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAIVLCAGDKSNDKRFYEKLVRIAE
                     DEFTAHLNTLESK"
     misc_feature    complement(8082..8426)
                     /locus_tag="YPC_4889"
                     /note="Uncharacterized protein conserved in bacteria
                     [Function unknown]; Region: COG4683"
                     /db_xref="CDD:227028"
     gene            8729..8830
                     /locus_tag="YPC_4890"
                     /db_xref="GeneID:12089378"
     CDS             8729..8830
                     /locus_tag="YPC_4890"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_005622068.1"
                     /db_xref="GI:384412705"
                     /db_xref="ASAP:BBE-0004850"
                     /db_xref="ASAP:BBE-0004850 ERIC"
                     /db_xref="GeneID:12089378"
                     /translation="MNGKTEFMPDERDLAIKAKHSNKDTPESRPDMF"
ORIGIN      
        1 tgtaacgaac ggtgcaatag tgatccacac ccaacgcctg aaatcagatc cagggggtag
       61 tctgctctcc tgattcagga gagtttatgg tcacttttga gacagttatg gaaattaaaa
      121 tcctgcacaa gcagggaatg agtagccggg cgattgccag agaactgggg atctcccgca
      181 ataccgttaa acgttatttg caggcaaaat ctgagccgcc aaaatatacg ccgcgacctg
      241 ctgttgcttc actcctggat gaataccggg attatattcg tcaacgcatc gccgatgctc
      301 atccttacaa aatcccggca acggtaatcg ctcgcgagat cagagaccag ggatatcgtg
      361 gcggaatgac cattctcagg gcattcattc gttctctctc ggttcctcag gagcaggagc
      421 ctgccgttcg gttcgaaact gaacccggac gacagatgca ggttgactgg ggcactatgc
      481 gtaatggtcg ctcaccgctt cacgtgttcg ttgctgttct cggatacagc cgaatgctgt
      541 acatcgaatt cactgacaat atgcgttatg acacgctgga gacctgccat cgtaatgcgt
      601 tccgcttctt tggtggtgtg ccgcgcgaag tgttgtatga caatatgaaa actgtggttc
      661 tgcaacgtga cgcatatcag accggtcagc accggttcca tccttcgctg tggcagttcg
      721 gcaaggagat gggcttctct ccccgactgt gtcgcccctt cagggcacag actaaaggta
      781 aggtggaacg gatggtgcag tacacccgta acagttttta catcccacta atgactcgcc
      841 tgcgcccgat ggggatcact gtcgatgttg aaacagccaa ccgccacggt ctgcgctggc
      901 tgcacgatgt cgctaaccaa cgaaaatcca ggcccgtccc tgcgatcgct ggctcgaaga
      961 gcagcagtcc atgctggcac tgcctccgga gaaaaaagag tatgacgtgc atcttgatga
     1021 aaatctggtg aacttcgaca aacaccccct gcatcatcca ctctccatct acgactcatt
     1081 ctgcagagga gtggcgtgat gatggaactg caacatcaac gactgatggc gctcgccggg
     1141 cagttgcaac tggaaagcct tataagcgca gcgcctgcgc tgtcacaaca ggcagtagac
     1201 caggaatgga gttatatgga cttcctggag catctgcttc atgaagaaaa actggcacgt
     1261 catcaacgta aacaggcgat gtatacccga atggcagcct tcccggcggt gaaaacgttc
     1321 gaagagtatg acttcacatt cgccaccgga gcaccgcaga agcaactcca gtcgttacgc
     1381 tcactcagct tcatagaacg taatgaaaat atcgtattac tggggccatc aggtgtgggg
     1441 aaaacccatc tggcaatagc gatgggctat gaagcagtcc gtgcaggtat caaagttcgc
     1501 ttcacaacag cagcagatct gttacttcag ttatctacgg cacaacgtca gggccgttat
     1561 aaaacgacgc ttcagcgtgg agtaatggcc ccccgcctgc tcatcattga tgaaataggc
     1621 tatctgccgt tcagtcagga agaagcaaag ctgttcttcc aggtcatcgc taaacgttac
     1681 gaaaagagcg caatgatcct gacatccaat ctgccgttcg ggcagtggga tcaaacgttc
     1741 gccggtgatg cagcactgac ctcagcgatg ctggaccgta tcttacacca ctcacatgtc
     1801 gttcaaatca aaggagaaag ctatcgactc agacagaaac gaaaggccgg ggttatagca
     1861 gaagctaatc ctgagtaaaa cggtggatca atattgggcc gttggtggag atataagtgg
     1921 atcacttttc atccgtcgtt gacaccctga tgaattcacg tgttcacgcc tgaataacaa
     1981 gaatgccgga gatacgcagt catatttttt acacaattct ctaatcccga caaggtcgta
     2041 ggtcgttata ggaaaattct tagcaccatt ccggaacaat cagaacagca ggccatgaac
     2101 gactgacaac attacgaata taaaaaacgc acccgggcca gacattcccc ctactgatta
     2161 aaccagccgg acttgtccac ggaacggtct ttttaaaccg acacacagtc tgagtacaga
     2221 tacatgtcac gatgatgcag gattagcgga agagtgtgag cacgtttccg ggaactgtgg
     2281 tgaaccatag ctcaatattc gagtgagggc ataccggaaa cgcgctcaga ttcgttgtaa
     2341 cgcgattttc cgtaccgggc aattttttca gttgtttttt cgtttcatgt cgtcagaaac
     2401 gttctgagcg cgtttccggc atctgatgct acgcaaacca tccccatggt cagttgacag
     2461 ccggaaacac gcgggtgtcg ttttagcgta tcgacgggac ggcgtcgaga agcacaaaaa
     2521 acagatgttg tactcagtca gttgttttac agacagcact gcggcagatt gaaaaagtac
     2581 cgtactttca ggaatgtcca gaaaccatgt gtcagacttc gttctccccc ttccgggtga
     2641 atttttttgt catccgttca ggaatctctt tataacgatt actccatttc aggatttttt
     2701 atgtggcgtt tactacaggc aggatattca aaggcaaaaa aatcccccgg aacaggcgga
     2761 acccggacag ggggagaacg aatcgctaaa taattttcgt agttgtattt cccatcgttg
     2821 ctactgcaac gggatgaatt tgccgcagtt tatcctgtaa aacaatcctg atttactcac
     2881 actccacata tcactgacgg agcacaacgg aatagtgaac aaacaacaac aaactgcgct
     2941 gaatatggcg cgatttatca gaagccagag cctgatactg cttgaaaaac tggatgctct
     3001 ggatgccgac gagcaggcgg ccatgtgtga acgactgcac gaactcgcgg aagaactcca
     3061 gaacagcatc caggctcgct ttgaagccga aagtgaaaca ggaacataac gaagctcccg
     3121 gagacggtca cagcttgtct gtgaacggat gccgggagca gacaagcccg tcagggcgcg
     3181 tcagcgggtt ttagcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
     3241 gtgtatactg gcttagtcat gcggcatcag tgcggattgt atgaaaagtg caccatgtac
     3301 ggtgtgaaat gccgcacaga tgcgtaagga gaacatgcag atgccgatgc tcttccgctt
     3361 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggtg tctgctcact
     3421 caaaagcggt gatactgtta tccacacaat caggggataa cgccggaaag aacatgtgag
     3481 caaaaaacga agaccccaga aaaggccgcg ccggaggcgc tttttccata ggctccgccc
     3541 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     3601 taaagatacc aggcgtttcc ccccggaagc tccctcgtgc gctctcctgt tccgaccctg
     3661 ccgcttaccg gatacctctc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3721 tcacgctgtt ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3781 gaaccccccg ttcagcccga ccactgcgcc ttatccggta actatcgtct tgagtccaac
     3841 ccggtaagac acgactttac gccactggca gcagccattg gtaactgaaa agtggattta
     3901 gatacgcaga actcttgaag ttgaagcctt atcgcggcta cactgaaagg acagcatttg
     3961 gtatctgtgc tccacttaag ccagctacca caggttagaa agcctgagaa acttctaacc
     4021 ttcgaaagaa cccacgcctg agaacgtggg ttttttcgtt tacaggcagc agattacgcg
     4081 cagaaaaaaa ggatctcaag aagatccttt gatcttttct actgaattgc gctcccgatc
     4141 agttcagcag aagattatga tggggttcta tgggtattgc tgcggtaaca cccatgttac
     4201 ttgaggttgt atgtagtctg tgtagaatta tacacataag gcttaaactg ctcttttttt
     4261 tcaatatgca attggaagtt cattgactac ataaatagat tattccaaat aatttattta
     4321 tgtaagaaca ggatgggagg gggaatgatc tcaaagttat tttgcttggc tctcatattt
     4381 ttatcatcaa gtggccttgc agaaaaaaac acatatacag caaaagacat cttgcaaaac
     4441 ctagaattaa atacctttgg caattcattg tctcatggca tctatgggaa acagacaacc
     4501 ttcaagcaaa ccgagtttac aaatattaaa agcaacacca aaaaacacat tgcacttatc
     4561 aataaagaca actcatggat gatatcatta aaaatactag gaattaagag agatgagtat
     4621 actgtctgtt ttgaagattt ctctctaata agaccgccaa catatgtagc catacatcct
     4681 ctacttataa aaaaagtaaa atctggaaac tttatagtag tgaaagaaat aaagaaatct
     4741 atccctggtt gcactgtata ttatcattaa tagcaagccc ctcattatta tgaggggctc
     4801 atggttattt taacaatcca ctatcgatat ctttttgcac cagagcgccc tctcgtttac
     4861 gtctgtcaga cattccatca acaatattat taaaagcatt tacaaggcca ttccagtctt
     4921 ttgcgataac tttattccat actgtgggag cagttctgga taacttaaac cctttttgat
     4981 atccaataga caccagtgct gtacgggttc tcaacggtaa atcgctgaac cgaagaccga
     5041 tattagcgtc attgaaaaga ccttcaatct tatgtgagaa tttatcaata taaatattag
     5101 ataagagatg agcttcatta tcagaaagcg tcagaggtgc tgttctcact ttatcataag
     5161 cctccttccc tcgaagcata taatacccat caagtctatc tgcaatatac tgagggacac
     5221 cgtcattcaa taaatcctgt ttgcttcgct gaccaaggtc aaccccggaa ccgaatgtaa
     5281 caccggtact gttaaaataa tcgctactag gattagacgg aaaatgactt gtcggattaa
     5341 acccttcaaa accattactg gagaaaatat cgtggtcaac aatatttacc gaacgacgta
     5401 aaaattcctt cagttgacta atattgtcaa agttaatgac agtgttgtcc gctaggacga
     5461 tgcgatttcg gttattattc agaatgtctt cgttctcttt cttatcgaga tgttcaatag
     5521 attcggcaat cgttccctca agaaccatga cacggtagac tttcacaccg tctttttcct
     5581 gacctgtttc aacagttatt ttctgttcgt aagacacggt cccttcagtt tttgaaattt
     5641 tactttcctg gcggatctta tttgaatatt cactgtcttt ctccatctcc gtatcaatcg
     5701 gaaaccccat aatgtacatc agtttaaaat tactccggcc aggcagatcc acataatgtg
     5761 gtaatgcaat tgtaatcgaa ttagcttcaa aatttggtct gtaactgctt aatgtacttc
     5821 cggaaaagag aaaagccgga acaccacctg aaccattcac taccattgta tctgacataa
     5881 aaattcctct ttaacacata aaaaaacaat aagttaaaaa aaaatactgt acataaaacc
     5941 actgttttta tgtacagtaa taaaattacg ccgctttatt ttctctgtca ataatatgaa
     6001 atttcatttt tgtgatctga atcactctta taaaaatcag gaagggaaga ttcgcagcag
     6061 aaaaacagca ccgggtaaca tcagaaaaaa acagaaagga gataacgtga gcaaaacaaa
     6121 atctggtcgc caccgactga gcaaaacaga caaacgcctg ctggctgcac ttgtcgttgc
     6181 cggatacgaa gaacggacag cccgtgacct catccagaaa cacgtttaca cactgacaca
     6241 ggccgacctg cgccatctgg tcagtgaaat cagtaacggt gtgggacagt cacaggccta
     6301 cgatgcgatt taccaggcga gacgcattcg tctcgcccgt aaatacctga gcggaaaaaa
     6361 accggaaggg gtggaacccc gggaagggca ggaacgggaa gatttaccat aactcccgtt
     6421 atcagtacca tcggctcaac gctcgttgtc ggatctgaaa aattcgctca aaagatcata
     6481 tttccctgga tattttccac cgtttcttat gtgagcaaag tcacataatt ctgtcagacg
     6541 acgagaaaac ggatatcgat tattgtttaa tatttttaca ttattaaaaa tgaaattaga
     6601 taatcagata caaataatat gttttcgttc atgcagagag attaagggtg tctaatgaag
     6661 aaaagttcta ttgtggcaac cattataact attctgtccg ggagtgctaa tgcagcatca
     6721 tctcagttaa taccaaatat atcccctgac agctttacag ttgcagcctc caccgggatg
     6781 ctgagtggaa agtctcatga aatgctttat gacgcagaaa caggaagaaa gatcagccag
     6841 ttagactgga agatcaaaaa tgtcgctatc ctgaaaggtg atatatcctg ggatccatac
     6901 tcatttctga ccctgaatgc cagggggtgg acgtctctgg cttccgggtc aggtaatatg
     6961 gatgactacg actggatgaa tgaaaatcaa tctgagtgga cagatcactc atctcatcct
     7021 gctacaaatg ttaatcatgc caatgaatat gacctcaatg tgaaaggctg gttactccag
     7081 gatgagaatt ataaagcagg tataacagca ggatatcagg aaacacgttt cagttggaca
     7141 gctacaggtg gttcatatag ttataataat ggagcttata ccggaaactt cccgaaagga
     7201 gtgcgggtaa taggttataa ccagcgcttt tctatgccat atattggact tgcaggccag
     7261 tatcgcatta atgattttga gttaaatgca ttatttaaat tcagcgactg ggttcgggca
     7321 catgataatg atgagcacta tatgagagat cttactttcc gtgagaagac atccggctca
     7381 cgttattatg gtaccgtaat taacgctgga tattatgtca cacctaatgc caaagtcttt
     7441 gcggaattta catacagtaa atatgatgag ggcaaaggag gtactcagac cattgataag
     7501 aatagtggag attctgtctc tattggcgga gatgctgccg gtatttccaa taaaaattat
     7561 actgtgacgg cgggtctgca atatcgcttc tgaaaaatac agatcatatc tctcttttca
     7621 tcctccccta gcggggagga tgtctgtgga aaggaggttg gtgtttgacc aaccttcaga
     7681 tgtgtgaaaa atcacctttt tcaccataat gacggggcgc tcattctgtt gttttgcctt
     7741 gacattctcc acgtctttca gggcatggag aaggtcaaat tagacatgga acgctactct
     7801 ccttcctgta ggaagctcaa catccaagct taatttgcct cccattgctt caacgtaacg
     7861 ctttaacgtc gccagcttta aatcatttcc gcgctgctcc agctttgtta ctgctggctg
     7921 gcttataccc atcgcctcag caacttgttt ttgtgataac tggagttctt cacgcatcat
     7981 ctgcaagccg acctcaagaa tcatctcatc tgccatttct ttaattcgtg tctggctttc
     8041 aggtgaacga ctggcaatca cctcatctaa tgttctcatt acttgctctc cagtgtgttc
     8101 agatgtgctg taaattcatc ctcagctata cgcaccagtt tttcataaaa ccgcttatca
     8161 ttacttttat ctcctgcaca aagaacgata gcccgacgaa tcggatcgaa cgcataaaag
     8221 gctcttatcg gacggccaga aaactgaacg cgaagctctt tcatattttt gtaccgagaa
     8281 cctttcacgg tatcggcata tggcctgggt aactcaggtc cgtaaacctg tagctttttc
     8341 aaatcagcca aaaccttttc ctgaagagcg tcttcttgct catttagcca gtcgtcaaat
     8401 cgctggctaa aaagtaccat ccacatgctc aaccctataa cctgtagctt accccactaa
     8461 caatataacc tacgagttat attttcaaga aaagctggct atttaacata acggcaattt
     8521 gtacgcacca ctgaaatgcg ttcagcgcga tcacggcaac agacaggcaa aaatagcaac
     8581 aaacctcccg aaaaaccgcc gcgatcgcgc ctgataaatt ttaaccttat gcatatctat
     8641 gcagccaggc gaatcacgaa cgaattgcct gcctgatgta actgaaacgg gtgttttttc
     8701 ctgatttggt gggcgtggaa gacggaacat gaacgggaaa acagaattca tgccagatga
     8761 gcgcgatctg gcaattaagg caaaacacag caacaaagac acgccagaat cgcgcccgga
     8821 tatgttttaa cgcgattttc agactcagac aaattcagca gaatgctact ccattcaccg
     8881 ggctgatggt gaatacatgc gtatccagga tgagtacatt tctggctctg ccacagctct
     8941 gtctgttggc agctttcgcc tgtccggaaa cctgcttaaa acgctcccga aaggcctctg
     9001 aaccagaaag caacaaaaca caggccatta agtaaatcgc gttaaaacac gtctgatgga
     9061 ttgctgcaaa aaaaagtccc taatggagca gggactgtta aacccagtga atagcgtcta
     9121 aattaaagta agaatacgac caggtactct tcagaaaaga gattaatcca ccgcacagaa
     9181 taatcaacag taaaaacaaa caaccctgat tttttatttt tctttttttc gataaaaaca
     9241 aaattaaaga aataattaat cagaacattc cttaacttca gggcattgcc tgtgttccat
     9301 tttgtgatta gtctgaaact tccgaaggtg gataacaccc ggtatttttt tgctcacata
     9361 aagcccctcc ttcaggcaga ggggcttttt ctttgccacc acataaaaaa ggccctcaca
     9421 ggaggtgttc tgtgagggcg tatgataagg actgaatcga tggttaatat gtctagtcct
     9481 gacttttgca tctccgaata taaaaccctg tttaacggca tgcaaaacca aaaaataaaa
     9541 atgtgacatc gcaatgccag ataatattga cgcatgaggg aatgcgtacc ccgacccctg
//
