locus-tag	class	description
GWK76_RS00005	COG0593	it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box) 5'-TTATC CA A CA A-3'. DnaA binds to ATP and to acidic phospholipids
GWK76_RS00010	COG0592	DNA-directed DNA polymerase activity
GWK76_RS00105	COG0071	Belongs to the small heat shock protein (HSP20) family
GWK76_RS00115	COG1576	Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA
GWK76_RS00170	COG0533	Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction
GWK76_RS00220	COG0770	UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity
GWK76_RS00235	COG0234	Binds to Cpn60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter
GWK76_RS00240	COG0459	protein refolding
GWK76_RS00255	COG2222	sugar isomerase
GWK76_RS00260	COG0577	efflux transmembrane transporter activity
GWK76_RS00265	COG1136	(ABC) transporter
GWK76_RS00290	COG0208	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides
GWK76_RS00305	COG0504	CTP synthase activity
GWK76_RS00315	COG0018	arginyl-trna synthetase
GWK76_RS00330	COG0528	UMP kinase activity
GWK76_RS00335	COG1949	3'-to-5' exoribonuclease specific for small oligoribonucleotides
GWK76_RS00345	COG2315	Protein conserved in bacteria
GWK76_RS00355	COG0358	DNA primase activity
GWK76_RS00360	COG0568	sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released
GWK76_RS00370	COG0258	In addition to polymerase activity, this DNA polymerase exhibits 5'-3' exonuclease activity
GWK76_RS00370	COG0749	In addition to polymerase activity, this DNA polymerase exhibits 5'-3' exonuclease activity
GWK76_RS00380	COG0266	class I DNA-(apurinic or apyrimidinic site) endonuclease activity
GWK76_RS00425	COG0184	Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome
GWK76_RS00445	COG1185	polyribonucleotide nucleotidyltransferase activity
GWK76_RS00455	COG1573	uracil-dna glycosylase
GWK76_RS00460	COG0595	An RNase that has 5'-3' exonuclease and possibly endonuclease activity. Involved in maturation of rRNA and in some organisms also mRNA maturation and or decay
GWK76_RS00480	COG0360	Binds together with S18 to 16S ribosomal RNA
GWK76_RS00485	COG0629	single-stranded DNA binding
GWK76_RS00490	COG0238	Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit
GWK76_RS00495	COG0231	Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase
GWK76_RS00500	COG1189	Ribosomal RNA methyltransferase RrmJ FtsJ
GWK76_RS00545	COG2804	Type II secretory pathway, ATPase PulE Tfp pilus assembly pathway, ATPase PilB
GWK76_RS00560	COG4969	Belongs to the N-Me-Phe pilin family
GWK76_RS00590	COG4974	Belongs to the 'phage' integrase family
GWK76_RS00635	COG0441	Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr)
GWK76_RS00645	COG3695	enzyme binding
GWK76_RS00650	COG0324	Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)
GWK76_RS00665	COG0227	Belongs to the bacterial ribosomal protein bL28 family
GWK76_RS00670	COG1994	Peptidase M50
GWK76_RS00680	COG0292	Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit
GWK76_RS00685	COG0291	Belongs to the bacterial ribosomal protein bL35 family
GWK76_RS00730	COG0187	DNA topoisomerase II activity
GWK76_RS00735	COG0188	A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner
GWK76_RS00775	COG0167	dihydroorotate dehydrogenase activity
GWK76_RS00815	COG0539	negative regulation of cytoplasmic translation
GWK76_RS00820	COG0532	One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex
GWK76_RS00835	COG0223	Attaches a formyl group to the free amino group of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus
GWK76_RS00840	COG0242	peptide deformylase activity
GWK76_RS00845	COG1198	Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA
GWK76_RS00855	COG0608	single-stranded DNA 5'-3' exodeoxyribonuclease activity
GWK76_RS00855	COG4199	Single-strand DNA-specific exonuclease, C terminal domain
GWK76_RS00870	COG1610	YqeY-like protein
GWK76_RS00875	COG0563	adenylate kinase activity
GWK76_RS00890	COG0206	Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity
GWK76_RS00895	COG1327	Negatively regulates transcription of bacterial ribonucleotide reductase nrd genes and operons by binding to NrdR- boxes
GWK76_RS00940	COG0060	amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile)
GWK76_RS00960	COG1192	Involved in chromosome partitioning
GWK76_RS00965	COG1475	DNA binding
GWK76_RS00985	COG0265	Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain
GWK76_RS00990	COG2890	protein-(glutamine-N5) methyltransferase activity
GWK76_RS01000	COG0216	Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA
GWK76_RS01010	COG0254	rRNA binding
GWK76_RS01020	COG0052	Belongs to the universal ribosomal protein uS2 family
GWK76_RS01025	COG0264	Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome
GWK76_RS01040	COG1195	it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP
GWK76_RS01050	COG0233	cytoplasmic translational termination
GWK76_RS01060	COG0020	Catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic pyrophosphates generating different type of terpenoids
GWK76_RS01075	COG0442	prolyl-tRNA aminoacylation
GWK76_RS01095	COG4535	Mg2 and Co2 transporter CorC
GWK76_RS01105	COG0577	efflux transmembrane transporter activity
GWK76_RS01105	COG1136	(ABC) transporter
GWK76_RS01110	COG1136	(ABC) transporter
GWK76_RS01115	COG1695	Transcriptional regulator
GWK76_RS01125	COG1968	undecaprenyl-diphosphatase activity
GWK76_RS01140	COG0172	Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec)
GWK76_RS01150	COG1544	Required for dimerization of active 70S ribosomes into 100S ribosomes in stationary phase
GWK76_RS01155	COG0653	protein targeting
GWK76_RS01165	COG0771	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)
GWK76_RS01175	COG0796	Provides the (R)-glutamate required for cell wall biosynthesis
GWK76_RS01180	COG1186	Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA
GWK76_RS01190	COG2884	Cell division ATP-binding protein ftsE
GWK76_RS01195	COG2177	Part of the ABC transporter FtsEX involved in
GWK76_RS01200	COG3942	CHAP domain
GWK76_RS01205	COG0793	Belongs to the peptidase S41A family
GWK76_RS01210	COG0745	RESPONSE REGULATOR receiver
GWK76_RS01240	COG1570	exodeoxyribonuclease VII activity
GWK76_RS01260	COG2205	protein histidine kinase activity
GWK76_RS01270	COG1381	Involved in DNA repair and RecF pathway recombination
GWK76_RS01275	COG0423	glycine-tRNA ligase activity
GWK76_RS01315	COG0019	diaminopimelate decarboxylase activity
GWK76_RS01325	COG0328	RNA-DNA hybrid ribonuclease activity
GWK76_RS01335	COG0507	A helicase nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit has ssDNA-dependent ATPase and 5'-3' helicase activity. When added to pre-assembled RecBC greatly stimulates nuclease activity and augments holoenzyme processivity. Negatively regulates the RecA-loading ability of RecBCD
GWK76_RS01340	COG2723	6-phospho-beta-galactosidase activity
GWK76_RS01345	COG2001	Belongs to the MraZ family
GWK76_RS01350	COG0275	rRNA processing
GWK76_RS01370	COG0472	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan
GWK76_RS01375	COG0772	Belongs to the SEDS family
GWK76_RS01380	COG0707	Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc- (pentapeptide)GlcNAc (lipid intermediate II)
GWK76_RS01420	COG0550	Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone
GWK76_RS01420	COG1754	DNA topoisomerase type I activity
GWK76_RS01435	COG0758	DNA mediated transformation
GWK76_RS01460	COG0701	Predicted permease
GWK76_RS01490	COG2265	Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family
GWK76_RS01515	COG1051	belongs to the nudix hydrolase family
GWK76_RS01525	COG1136	(ABC) transporter
GWK76_RS01580	COG0456	acetyltransferase
GWK76_RS01580	COG2320	Catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A
GWK76_RS01585	COG5002	protein histidine kinase activity
GWK76_RS01590	COG0745	RESPONSE REGULATOR receiver
GWK76_RS01595	COG0782	Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus
GWK76_RS01600	COG5002	protein histidine kinase activity
GWK76_RS01605	COG0745	RESPONSE REGULATOR receiver
GWK76_RS01615	COG4152	abc transporter atp-binding protein
GWK76_RS01645	COG1160	GTP binding
GWK76_RS01655	COG1187	pseudouridine synthase activity
GWK76_RS01660	COG0745	RESPONSE REGULATOR receiver
GWK76_RS01665	COG5002	protein histidine kinase activity
GWK76_RS01675	COG0577	efflux transmembrane transporter activity
GWK76_RS01675	COG1136	(ABC) transporter
GWK76_RS01680	COG0456	acetyltransferase
GWK76_RS01715	COG0494	nUDIX hydrolase
GWK76_RS01735	COG0745	RESPONSE REGULATOR receiver
GWK76_RS01740	COG5002	protein histidine kinase activity
GWK76_RS01745	COG1470	cell adhesion involved in biofilm formation
GWK76_RS01750	COG0008	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)
GWK76_RS01750	COG1384	Belongs to the class-I aminoacyl-tRNA synthetase family
GWK76_RS01790	COG1876	peptidase activity
GWK76_RS01795	COG1876	peptidase activity
GWK76_RS01800	COG0708	Exodeoxyribonuclease III
GWK76_RS01805	COG0297	glycogen (starch) synthase activity
GWK76_RS01805	COG0438	transferase activity, transferring glycosyl groups
GWK76_RS01830	COG0488	(ABC) transporter
GWK76_RS01865	COG0712	F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation
GWK76_RS01870	COG0056	Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit
GWK76_RS01875	COG0224	Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex
GWK76_RS01885	COG0055	Produces ATP from ADP in the presence of a proton gradient across the membrane. The catalytic sites are hosted primarily by the beta subunits
GWK76_RS01895	COG0042	Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines
GWK76_RS01950	COG1136	(ABC) transporter
GWK76_RS01955	COG0353	May play a role in DNA repair. It seems to be involved in an RecBC-independent recombinational process of DNA repair. It may act with RecF and RecO
GWK76_RS01960	COG0718	Binds to DNA and alters its conformation. May be involved in regulation of gene expression, nucleoid organization and DNA protection
GWK76_RS01970	COG0305	Participates in initiation and elongation during chromosome replication
GWK76_RS01975	COG2812	DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity
GWK76_RS01990	COG0222	ribosome binding
GWK76_RS01995	COG0244	Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors
GWK76_RS02000	COG0081	regulation of translation
GWK76_RS02010	COG0148	phosphopyruvate hydratase activity
GWK76_RS02015	COG0009	L-threonylcarbamoyladenylate synthase
GWK76_RS02020	COG0080	Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors
GWK76_RS02030	COG0250	Participates in transcription elongation, termination and antitermination
GWK76_RS02040	COG5002	protein histidine kinase activity
GWK76_RS02080	COG0267	Belongs to the bacterial ribosomal protein bL33 family
GWK76_RS02090	COG0778	Nitroreductase
GWK76_RS02140	COG0470	DNA polymerase III
GWK76_RS02145	COG0564	pseudouridine synthase activity
GWK76_RS02150	COG0180	Tryptophanyl-tRNA synthetase
GWK76_RS02175	COG0103	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
GWK76_RS02180	COG0102	This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly
GWK76_RS02185	COG0203	ribosomal protein L17
GWK76_RS02190	COG0202	RNA polymerase activity
GWK76_RS02195	COG0522	One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit
GWK76_RS02200	COG0100	Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome
GWK76_RS02210	COG0257	Belongs to the bacterial ribosomal protein bL36 family
GWK76_RS02215	COG0361	One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre- initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initation complex
GWK76_RS02230	COG0200	Binds to the 23S rRNA
GWK76_RS02235	COG0098	Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body
GWK76_RS02245	COG0256	This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance
GWK76_RS02250	COG0097	This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7 L12 stalk, and near the tRNA binding site of the peptidyltransferase center
GWK76_RS02265	COG0127	Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA RNA and avoiding chromosomal lesions
GWK76_RS02270	COG0096	One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit
GWK76_RS02275	COG0199	Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site
GWK76_RS02285	COG0094	This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits
GWK76_RS02290	COG0198	One of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit
GWK76_RS02295	COG0093	Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome
GWK76_RS02300	COG0186	One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA
GWK76_RS02305	COG0255	Belongs to the universal ribosomal protein uL29 family
GWK76_RS02310	COG0197	Binds 23S rRNA and is also seen to make contacts with the A and possibly P site tRNAs
GWK76_RS02315	COG0092	Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation
GWK76_RS02320	COG0091	The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome
GWK76_RS02325	COG0185	Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA
GWK76_RS02330	COG0090	One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity
GWK76_RS02335	COG0089	rRNA binding
GWK76_RS02340	COG0088	Forms part of the polypeptide exit tunnel
GWK76_RS02345	COG0087	One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit
GWK76_RS02355	COG0480	Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome
GWK76_RS02365	COG1666	GTP binding
GWK76_RS02370	COG0049	tRNA binding
GWK76_RS02375	COG0048	Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit
GWK76_RS02380	COG0086	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates
GWK76_RS02385	COG0085	RNA polymerase activity
GWK76_RS02395	COG0125	Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis
GWK76_RS02410	COG0207	Catalyzes the reductive methylation of 2'-deoxyuridine- 5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis
GWK76_RS02415	COG0740	serine-type endopeptidase activity
GWK76_RS02420	COG0544	peptidyl-prolyl cis-trans isomerase activity
GWK76_RS02450	COG0277	FAD linked oxidase domain protein
GWK76_RS02495	COG2256	atpase related to the helicase subunit of the holliday junction resolvase
GWK76_RS02505	COG1181	Belongs to the D-alanine--D-alanine ligase family
GWK76_RS02510	COG0330	HflC and HflK could
GWK76_RS02550	COG0366	Alpha amylase catalytic
GWK76_RS02565	COG0742	ribosomal rna small subunit methyltransferase
GWK76_RS02575	COG1200	Critical role in recombination and DNA repair. Helps process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-DNA)
GWK76_RS02585	COG0162	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)
GWK76_RS02590	COG0744	Peptidoglycan polymerase that catalyzes glycan chain elongation from lipid-linked precursors
GWK76_RS02595	COG4956	integral membrane protein (PIN domain superfamily)
GWK76_RS02600	COG1066	DNA-dependent ATPase involved in processing of recombination intermediates, plays a role in repairing DNA breaks. Stimulates the branch migration of RecA-mediated strand transfer reactions, allowing the 3' invading strand to extend heteroduplex DNA faster. Binds ssDNA in the presence of ADP but not other nucleotides, has ATPase activity that is stimulated by ssDNA and various branched DNA structures, but inhibited by SSB. Does not have RecA's homology-searching function
GWK76_RS02610	COG0542	response to heat
GWK76_RS02615	COG0195	Participates in both transcription termination and antitermination
GWK76_RS02640	COG0492	ferredoxin-NADP+ reductase activity
GWK76_RS02650	COG1132	(ABC) transporter
GWK76_RS02655	COG0073	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation
GWK76_RS02655	COG0072	phenylalanine-tRNA ligase activity
GWK76_RS02700	COG0580	Belongs to the MIP aquaporin (TC 1.A.8) family
GWK76_RS02735	COG0336	Belongs to the RNA methyltransferase TrmD family
GWK76_RS02740	COG1837	Belongs to the UPF0109 family
GWK76_RS02745	COG1559	Functions as a peptidoglycan terminase that cleaves nascent peptidoglycan strands endolytically to terminate their elongation
GWK76_RS02750	COG0816	Could be a nuclease involved in processing of the 5'-end of pre-16S rRNA
GWK76_RS02760	COG0849	cell division
GWK76_RS02770	COG0013	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain
GWK76_RS02780	COG0228	four-way junction DNA binding
GWK76_RS02800	COG1104	cysteine desulfurase activity
GWK76_RS02815	COG0084	hydrolase, TatD family'
GWK76_RS02820	COG0143	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation
GWK76_RS02830	COG0030	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity
GWK76_RS02860	COG0149	triose-phosphate isomerase activity
GWK76_RS02865	COG0126	phosphoglycerate kinase activity
GWK76_RS02875	COG0571	ribonuclease III activity
GWK76_RS02900	COG0495	leucyl-tRNA aminoacylation
GWK76_RS02910	COG0770	UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity
GWK76_RS02920	COG0280	phosphate acetyltransferase
GWK76_RS02935	COG0127	Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA RNA and avoiding chromosomal lesions
GWK76_RS02950	COG0272	DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA
GWK76_RS02960	COG0501	Belongs to the peptidase M48B family
GWK76_RS02965	COG1704	LemA family
GWK76_RS02975	COG1131	(ABC) transporter
GWK76_RS03005	COG0747	negative chemotaxis
GWK76_RS03015	COG1950	PFAM Membrane protein of
GWK76_RS03055	COG1210	Utp--glucose-1-phosphate uridylyltransferase
GWK76_RS03065	COG0064	Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln)
GWK76_RS03075	COG0154	amidase activity
GWK76_RS03095	COG0556	nucleotide-excision repair
GWK76_RS03125	COG0587	DNA-directed DNA polymerase activity
GWK76_RS03130	COG0587	DNA-directed DNA polymerase activity
GWK76_RS03140	COG0587	DNA-directed DNA polymerase activity
GWK76_RS03160	COG1210	Utp--glucose-1-phosphate uridylyltransferase
GWK76_RS03180	COG0154	amidase activity
GWK76_RS03200	COG0556	nucleotide-excision repair
GWK76_RS03205	COG1611	Belongs to the LOG family
GWK76_RS03215	COG0524	Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5- phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway
GWK76_RS03225	COG1154	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP)
GWK76_RS03230	COG3959	Transketolase, thiamine diphosphate binding domain
GWK76_RS03240	COG0698	ribose 5-phosphate isomerase
GWK76_RS03260	COG0164	Endonuclease that specifically degrades the RNA of RNA- DNA hybrids
GWK76_RS03270	COG0335	large ribosomal subunit rRNA binding
GWK76_RS03275	COG0281	malic enzyme
GWK76_RS03280	COG2804	Type II secretory pathway, ATPase PulE Tfp pilus assembly pathway, ATPase PilB
GWK76_RS03300	COG0051	transcription antitermination factor activity, RNA binding
GWK76_RS03325	COG0449	glutamine-fructose-6-phosphate transaminase (isomerizing) activity
GWK76_RS03330	COG0343	queuine tRNA-ribosyltransferase activity
GWK76_RS03335	COG0296	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position
GWK76_RS03340	COG1573	uracil-dna glycosylase
GWK76_RS03365	COG0242	peptide deformylase activity
GWK76_RS03370	COG1132	(ABC) transporter
GWK76_RS03375	COG0586	Pfam SNARE associated Golgi protein
GWK76_RS03380	COG0178	The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by uvrB, the uvrA molecules dissociate
GWK76_RS03400	COG1136	(ABC) transporter
GWK76_RS03415	COG2925	Exonuclease
GWK76_RS03420	COG0536	An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control
GWK76_RS03425	COG3942	CHAP domain
GWK76_RS03425	COG1388	LysM domain
GWK76_RS03450	COG5002	protein histidine kinase activity
GWK76_RS03460	COG2204	response regulator
GWK76_RS03465	COG0190	Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate
GWK76_RS03500	COG0766	Cell wall formation. Adds enolpyruvyl to UDP-N- acetylglucosamine
GWK76_RS03505	COG0112	Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF- independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism
GWK76_RS03530	COG4968	Prepilin-type N-terminal cleavage methylation domain
GWK76_RS03535	COG0812	UDP-N-acetylmuramate dehydrogenase activity
GWK76_RS03555	COG0542	response to heat
GWK76_RS03595	COG0588	phosphoglycerate mutase activity
GWK76_RS03605	COG1418	mRNA catabolic process
GWK76_RS03610	COG1214	PFAM Peptidase M22, glycoprotease
GWK76_RS03615	COG0802	atpase or kinase
GWK76_RS03645	COG1459	type II secretion system
GWK76_RS03665	COG2954	triphosphatase activity
GWK76_RS03670	COG0220	tRNA (guanine-N7-)-methyltransferase activity
GWK76_RS03675	COG0668	transmembrane transport
GWK76_RS03700	COG1194	a g-specific adenine glycosylase
GWK76_RS03725	COG0173	Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp Asn)
GWK76_RS03730	COG2255	four-way junction helicase activity
GWK76_RS03750	COG0632	four-way junction helicase activity
GWK76_RS03755	COG0744	Peptidoglycan polymerase that catalyzes glycan chain elongation from lipid-linked precursors
GWK76_RS03760	COG0817	Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group
GWK76_RS03775	COG0217	transcriptional regulatory protein
GWK76_RS03805	COG0189	ligase activity
GWK76_RS03835	COG0443	Heat shock 70 kDa protein
GWK76_RS03840	COG0576	Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ
GWK76_RS03850	COG1420	regulation of RNA biosynthetic process
GWK76_RS03860	COG0194	GDP metabolic process
GWK76_RS03880	COG0481	GTPase activity
GWK76_RS03935	COG0465	Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins
GWK76_RS03940	COG0037	tRNA processing
GWK76_RS03970	COG1249	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family
GWK76_RS04025	COG0773	UDP-N-acetylmuramate-L-alanine ligase activity
GWK76_RS04030	COG2137	regulation of response to DNA damage stimulus
GWK76_RS04040	COG0468	Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage
GWK76_RS04060	COG1651	Required for disulfide bond formation in some periplasmic proteins. Acts by transferring its disulfide bond to other proteins and is reduced in the process
GWK76_RS04060	COG3004	Na( ) H( ) antiporter that extrudes sodium in exchange for external protons
GWK76_RS04075	COG0477	Major facilitator Superfamily
GWK76_RS04120	COG1288	antiporter activity
GWK76_RS04125	COG0078	Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline
GWK76_RS04185	COG0765	amino acid transport
GWK76_RS04190	COG0834	ABC-type amino acid transport signal transduction systems periplasmic component domain
GWK76_RS04200	COG1087	Belongs to the NAD(P)-dependent epimerase dehydratase family
GWK76_RS04205	COG0542	response to heat
GWK76_RS04215	COG1136	(ABC) transporter
GWK76_RS04235	COG0726	polysaccharide deacetylase
GWK76_RS04240	COG1872	DUF167
GWK76_RS04245	COG3620	sequence-specific DNA binding
GWK76_RS04250	COG0610	Subunit R is required for both nuclease and ATPase activities, but not for modification
GWK76_RS04265	COG1195	it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP
GWK76_RS04270	COG0732	type I restriction modification DNA specificity domain
GWK76_RS04285	COG0286	site-specific DNA-methyltransferase (adenine-specific) activity
GWK76_RS04290	COG2003	Belongs to the UPF0758 family
GWK76_RS04315	COG1328	CTP reductase activity
GWK76_RS04315	COG1327	Negatively regulates transcription of bacterial ribonucleotide reductase nrd genes and operons by binding to NrdR- boxes
GWK76_RS04330	COG1131	(ABC) transporter
GWK76_RS04335	COG5002	protein histidine kinase activity
GWK76_RS04335	COG3850	Histidine kinase
GWK76_RS04345	COG1619	proteins homologs of microcin C7 resistance protein MccF
GWK76_RS04375	COG1807	4-amino-4-deoxy-L-arabinose transferase and related glycosyltransferases of PMT family
GWK76_RS04400	COG1974	Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair
GWK76_RS04425	COG0662	Cupin 2, conserved barrel domain protein
GWK76_RS04425	COG0836	Belongs to the mannose-6-phosphate isomerase type 2 family
GWK76_RS04430	COG1088	dTDP-glucose 4,6-dehydratase activity
GWK76_RS04460	COG1215	transferase activity, transferring glycosyl groups
GWK76_RS04485	COG0399	UDP-4-amino-4-deoxy-L-arabinose aminotransferase
GWK76_RS04505	COG1216	Glycosyl transferase, family 2
GWK76_RS04505	COG0438	transferase activity, transferring glycosyl groups
GWK76_RS04510	COG1216	Glycosyl transferase, family 2
GWK76_RS04510	COG0438	transferase activity, transferring glycosyl groups
GWK76_RS04545	COG0594	ribonuclease P activity
GWK76_RS04550	COG0230	Belongs to the bacterial ribosomal protein bL34 family