locus-tag	class	description
GWK75_RS00005	COG0593	it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box) 5'-TTATC CA A CA A-3'. DnaA binds to ATP and to acidic phospholipids
GWK75_RS00010	COG0592	DNA-directed DNA polymerase activity
GWK75_RS00015	COG1195	it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP
GWK75_RS00110	COG1040	competence protein
GWK75_RS00160	COG1576	Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA
GWK75_RS00170	COG0525	valine-tRNA ligase activity
GWK75_RS00185	COG0533	Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction
GWK75_RS00210	COG2222	sugar isomerase
GWK75_RS00215	COG1136	(ABC) transporter
GWK75_RS00215	COG0577	efflux transmembrane transporter activity
GWK75_RS00220	COG0577	efflux transmembrane transporter activity
GWK75_RS00220	COG1136	(ABC) transporter
GWK75_RS00250	COG4152	abc transporter atp-binding protein
GWK75_RS00270	COG0504	CTP synthase activity
GWK75_RS00280	COG0018	arginyl-trna synthetase
GWK75_RS00290	COG1949	3'-to-5' exoribonuclease specific for small oligoribonucleotides
GWK75_RS00295	COG2315	Protein conserved in bacteria
GWK75_RS00305	COG0358	DNA primase activity
GWK75_RS00305	COG0305	Participates in initiation and elongation during chromosome replication
GWK75_RS00320	COG0258	In addition to polymerase activity, this DNA polymerase exhibits 5'-3' exonuclease activity
GWK75_RS00320	COG0749	In addition to polymerase activity, this DNA polymerase exhibits 5'-3' exonuclease activity
GWK75_RS00325	COG0756	dUTP diphosphatase activity
GWK75_RS00350	COG0546	phosphoglycolate phosphatase activity
GWK75_RS00355	COG0266	class I DNA-(apurinic or apyrimidinic site) endonuclease activity
GWK75_RS00365	COG0268	Binds directly to 16S ribosomal RNA
GWK75_RS00375	COG0130	Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs
GWK75_RS00385	COG0184	Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome
GWK75_RS00395	COG0782	Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus
GWK75_RS00405	COG1185	polyribonucleotide nucleotidyltransferase activity
GWK75_RS00415	COG1573	uracil-dna glycosylase
GWK75_RS00420	COG0595	An RNase that has 5'-3' exonuclease and possibly endonuclease activity. Involved in maturation of rRNA and in some organisms also mRNA maturation and or decay
GWK75_RS00430	COG1605	Chorismate mutase
GWK75_RS00430	COG0077	Prephenate dehydratase
GWK75_RS00435	COG0360	Binds together with S18 to 16S ribosomal RNA
GWK75_RS00445	COG0238	Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit
GWK75_RS00450	COG0231	Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase
GWK75_RS00455	COG1189	Ribosomal RNA methyltransferase RrmJ FtsJ
GWK75_RS00460	COG0012	GTP binding
GWK75_RS00495	COG2804	Type II secretory pathway, ATPase PulE Tfp pilus assembly pathway, ATPase PilB
GWK75_RS00515	COG1989	Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue
GWK75_RS00540	COG4974	Belongs to the 'phage' integrase family
GWK75_RS00600	COG0441	Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr)
GWK75_RS00610	COG0324	Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)
GWK75_RS00625	COG0227	Belongs to the bacterial ribosomal protein bL28 family
GWK75_RS00630	COG1994	Peptidase M50
GWK75_RS00640	COG0292	Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit
GWK75_RS00650	COG0290	IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins
GWK75_RS00655	COG0606	chelatase, subunit chli
GWK75_RS00695	COG0187	DNA topoisomerase II activity
GWK75_RS00700	COG0188	A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner
GWK75_RS00765	COG0539	negative regulation of cytoplasmic translation
GWK75_RS00770	COG0532	One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex
GWK75_RS00770	COG3064	Membrane
GWK75_RS00795	COG0223	Attaches a formyl group to the free amino group of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus
GWK75_RS00800	COG0242	peptide deformylase activity
GWK75_RS00805	COG1198	Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA
GWK75_RS00820	COG4199	Single-strand DNA-specific exonuclease, C terminal domain
GWK75_RS00820	COG0608	single-stranded DNA 5'-3' exodeoxyribonuclease activity
GWK75_RS00840	COG1610	YqeY-like protein
GWK75_RS00845	COG0563	adenylate kinase activity
GWK75_RS00850	COG0024	Methionine aminopeptidase
GWK75_RS00860	COG0206	Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity
GWK75_RS00865	COG1327	Negatively regulates transcription of bacterial ribonucleotide reductase nrd genes and operons by binding to NrdR- boxes
GWK75_RS00885	COG0060	amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile)
GWK75_RS00895	COG1983	PspC domain protein
GWK75_RS00905	COG0261	This protein binds to 23S rRNA in the presence of protein L20
GWK75_RS00910	COG1192	Involved in chromosome partitioning
GWK75_RS00915	COG1475	DNA binding
GWK75_RS00925	COG1316	TRANSCRIPTIONal
GWK75_RS00935	COG0265	Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain
GWK75_RS00940	COG2890	protein-(glutamine-N5) methyltransferase activity
GWK75_RS00945	COG0216	Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA
GWK75_RS00950	COG0254	rRNA binding
GWK75_RS00955	COG1051	belongs to the nudix hydrolase family
GWK75_RS00965	COG0052	Belongs to the universal ribosomal protein uS2 family
GWK75_RS00970	COG0264	Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome
GWK75_RS00975	COG0233	cytoplasmic translational termination
GWK75_RS00980	COG0020	Catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic pyrophosphates generating different type of terpenoids
GWK75_RS00995	COG0442	prolyl-tRNA aminoacylation
GWK75_RS01000	COG0782	Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus
GWK75_RS01010	COG1190	Belongs to the class-II aminoacyl-tRNA synthetase family
GWK75_RS01020	COG1968	undecaprenyl-diphosphatase activity
GWK75_RS01025	COG1136	(ABC) transporter
GWK75_RS01030	COG0577	efflux transmembrane transporter activity
GWK75_RS01040	COG0172	Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec)
GWK75_RS01045	COG0167	dihydroorotate dehydrogenase activity
GWK75_RS01055	COG0653	protein targeting
GWK75_RS01065	COG0771	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)
GWK75_RS01075	COG1186	Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA
GWK75_RS01080	COG1704	LemA family
GWK75_RS01090	COG2884	Cell division ATP-binding protein ftsE
GWK75_RS01095	COG2177	Part of the ABC transporter FtsEX involved in
GWK75_RS01105	COG0793	Belongs to the peptidase S41A family
GWK75_RS01110	COG0745	RESPONSE REGULATOR receiver
GWK75_RS01120	COG0211	Belongs to the bacterial ribosomal protein bL27 family
GWK75_RS01135	COG1570	exodeoxyribonuclease VII activity
GWK75_RS01165	COG1381	Involved in DNA repair and RecF pathway recombination
GWK75_RS01210	COG1190	Belongs to the class-II aminoacyl-tRNA synthetase family
GWK75_RS01235	COG0328	RNA-DNA hybrid ribonuclease activity
GWK75_RS01240	COG0192	Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme
GWK75_RS01245	COG0507	A helicase nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit has ssDNA-dependent ATPase and 5'-3' helicase activity. When added to pre-assembled RecBC greatly stimulates nuclease activity and augments holoenzyme processivity. Negatively regulates the RecA-loading ability of RecBCD
GWK75_RS01270	COG2723	6-phospho-beta-galactosidase activity
GWK75_RS01275	COG0391	Required for morphogenesis under gluconeogenic growth conditions
GWK75_RS01280	COG0275	rRNA processing
GWK75_RS01295	COG0472	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan
GWK75_RS01300	COG0772	Belongs to the SEDS family
GWK75_RS01340	COG3512	CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease. Involved in the integration of spacer DNA into the CRISPR cassette
GWK75_RS01345	COG1518	maintenance of DNA repeat elements
GWK75_RS01365	COG3513	CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9 crRNA tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer
GWK75_RS01420	COG0550	Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone
GWK75_RS01425	COG1754	DNA topoisomerase type I activity
GWK75_RS01425	COG0550	Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone
GWK75_RS01470	COG0472	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan
GWK75_RS01475	COG0472	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan
GWK75_RS01490	COG0772	Belongs to the SEDS family
GWK75_RS01505	COG0707	Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc- (pentapeptide)GlcNAc (lipid intermediate II)
GWK75_RS01565	COG4886	Leucine-rich repeat (LRR) protein
GWK75_RS01600	COG3512	CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease. Involved in the integration of spacer DNA into the CRISPR cassette
GWK75_RS01605	COG1518	maintenance of DNA repeat elements
GWK75_RS01610	COG3513	CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9 crRNA tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer
GWK75_RS01650	COG0758	DNA mediated transformation
GWK75_RS01660	COG0193	aminoacyl-tRNA hydrolase activity
GWK75_RS01675	COG1160	GTP binding
GWK75_RS01705	COG1187	pseudouridine synthase activity
GWK75_RS01710	COG0745	RESPONSE REGULATOR receiver
GWK75_RS01715	COG5002	protein histidine kinase activity
GWK75_RS01720	COG0501	Belongs to the peptidase M48B family
GWK75_RS01770	COG2173	Catalyzes hydrolysis of the D-alanyl-D-alanine dipeptide
GWK75_RS01785	COG1136	(ABC) transporter
GWK75_RS01790	COG0745	RESPONSE REGULATOR receiver
GWK75_RS01795	COG5002	protein histidine kinase activity
GWK75_RS01800	COG1470	cell adhesion involved in biofilm formation
GWK75_RS01850	COG0708	Exodeoxyribonuclease III
GWK75_RS01860	COG0283	belongs to the cytidylate kinase family. Type 1 subfamily
GWK75_RS01875	COG0438	transferase activity, transferring glycosyl groups
GWK75_RS01880	COG0691	Required for rescue of stalled ribosomes mediated by trans-translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene
GWK75_RS01890	COG0488	(ABC) transporter
GWK75_RS01915	COG0356	it plays a direct role in the translocation of protons across the membrane
GWK75_RS01935	COG0056	Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit
GWK75_RS01940	COG0224	Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex
GWK75_RS01950	COG0055	Produces ATP from ADP in the presence of a proton gradient across the membrane. The catalytic sites are hosted primarily by the beta subunits
GWK75_RS01955	COG0355	proton-transporting ATP synthase activity, rotational mechanism
GWK75_RS01960	COG0042	Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines
GWK75_RS01975	COG2165	general secretion pathway protein
GWK75_RS01980	COG2265	Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family
GWK75_RS01985	COG0282	Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction
GWK75_RS01990	COG3957	D-xylulose 5-phosphate D-fructose 6-phosphate phosphoketolase
GWK75_RS02030	COG0215	cysteine-tRNA ligase activity
GWK75_RS02045	COG0353	May play a role in DNA repair. It seems to be involved in an RecBC-independent recombinational process of DNA repair. It may act with RecF and RecO
GWK75_RS02050	COG0718	Binds to DNA and alters its conformation. May be involved in regulation of gene expression, nucleoid organization and DNA protection
GWK75_RS02060	COG0305	Participates in initiation and elongation during chromosome replication
GWK75_RS02065	COG2812	DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity
GWK75_RS02070	COG1435	thymidine kinase activity
GWK75_RS02085	COG0222	ribosome binding
GWK75_RS02090	COG0244	Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors
GWK75_RS02100	COG0125	Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis
GWK75_RS02115	COG0207	Catalyzes the reductive methylation of 2'-deoxyuridine- 5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis
GWK75_RS02130	COG0081	regulation of translation
GWK75_RS02135	COG3942	CHAP domain
GWK75_RS02170	COG0080	Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors
GWK75_RS02180	COG0250	Participates in transcription elongation, termination and antitermination
GWK75_RS02190	COG5002	protein histidine kinase activity
GWK75_RS02225	COG0438	transferase activity, transferring glycosyl groups
GWK75_RS02245	COG0267	Belongs to the bacterial ribosomal protein bL33 family
GWK75_RS02250	COG1666	GTP binding
GWK75_RS02265	COG0378	Facilitates the functional incorporation of the urease nickel metallocenter. This process requires GTP hydrolysis, probably effectuated by UreG
GWK75_RS02315	COG0564	pseudouridine synthase activity
GWK75_RS02320	COG0180	Tryptophanyl-tRNA synthetase
GWK75_RS02325	COG2267	carboxylic ester hydrolase activity
GWK75_RS02330	COG0103	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
GWK75_RS02335	COG0102	This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly
GWK75_RS02340	COG0203	ribosomal protein L17
GWK75_RS02345	COG0202	RNA polymerase activity
GWK75_RS02350	COG0522	One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit
GWK75_RS02355	COG0100	Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome
GWK75_RS02385	COG0200	Binds to the 23S rRNA
GWK75_RS02390	COG0098	Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body
GWK75_RS02395	COG0256	This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance
GWK75_RS02400	COG0097	This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7 L12 stalk, and near the tRNA binding site of the peptidyltransferase center
GWK75_RS02410	COG0096	One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit
GWK75_RS02415	COG0199	Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site
GWK75_RS02420	COG0094	This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits
GWK75_RS02425	COG0198	One of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit
GWK75_RS02440	COG0255	Belongs to the universal ribosomal protein uL29 family
GWK75_RS02445	COG0197	Binds 23S rRNA and is also seen to make contacts with the A and possibly P site tRNAs
GWK75_RS02460	COG0185	Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA
GWK75_RS02465	COG0090	One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity
GWK75_RS02475	COG0088	Forms part of the polypeptide exit tunnel
GWK75_RS02480	COG0087	One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit
GWK75_RS02495	COG0051	transcription antitermination factor activity, RNA binding
GWK75_RS02505	COG0549	Belongs to the carbamate kinase family
GWK75_RS02510	COG1288	antiporter activity
GWK75_RS02525	COG0050	This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis
GWK75_RS02530	COG1087	Belongs to the NAD(P)-dependent epimerase dehydratase family
GWK75_RS02555	COG0480	Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome
GWK75_RS02580	COG0049	tRNA binding
GWK75_RS02585	COG0048	Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit
GWK75_RS02605	COG0085	RNA polymerase activity
GWK75_RS02615	COG0740	serine-type endopeptidase activity
GWK75_RS02620	COG0544	peptidyl-prolyl cis-trans isomerase activity
GWK75_RS02655	COG0277	FAD linked oxidase domain protein
GWK75_RS02675	COG0742	ribosomal rna small subunit methyltransferase
GWK75_RS02685	COG1200	Critical role in recombination and DNA repair. Helps process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-DNA)
GWK75_RS02695	COG0162	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)
GWK75_RS02700	COG0744	Peptidoglycan polymerase that catalyzes glycan chain elongation from lipid-linked precursors
GWK75_RS02705	COG4956	integral membrane protein (PIN domain superfamily)
GWK75_RS02710	COG1066	DNA-dependent ATPase involved in processing of recombination intermediates, plays a role in repairing DNA breaks. Stimulates the branch migration of RecA-mediated strand transfer reactions, allowing the 3' invading strand to extend heteroduplex DNA faster. Binds ssDNA in the presence of ADP but not other nucleotides, has ATPase activity that is stimulated by ssDNA and various branched DNA structures, but inhibited by SSB. Does not have RecA's homology-searching function
GWK75_RS02720	COG0542	response to heat
GWK75_RS02725	COG0195	Participates in both transcription termination and antitermination
GWK75_RS02735	COG0809	Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)
GWK75_RS02765	COG0164	Endonuclease that specifically degrades the RNA of RNA- DNA hybrids
GWK75_RS02770	COG0335	large ribosomal subunit rRNA binding
GWK75_RS02775	COG0281	malic enzyme
GWK75_RS02780	COG2804	Type II secretory pathway, ATPase PulE Tfp pilus assembly pathway, ATPase PilB
GWK75_RS02840	COG0154	amidase activity
GWK75_RS02845	COG0721	Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln)
GWK75_RS02850	COG0296	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position
GWK75_RS02855	COG0296	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position
GWK75_RS02860	COG0556	nucleotide-excision repair
GWK75_RS02875	COG0524	Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5- phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway
GWK75_RS02885	COG3958	Transketolase
GWK75_RS02890	COG0021	Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate
GWK75_RS02905	COG0057	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
GWK75_RS02930	COG0584	glycerophosphoryl diester phosphodiesterase
GWK75_RS02935	COG0492	ferredoxin-NADP+ reductase activity
GWK75_RS02945	COG1132	(ABC) transporter
GWK75_RS02960	COG0072	phenylalanine-tRNA ligase activity
GWK75_RS02960	COG0073	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation
GWK75_RS02965	COG1051	belongs to the nudix hydrolase family
GWK75_RS02980	COG0580	Belongs to the MIP aquaporin (TC 1.A.8) family
GWK75_RS03015	COG1327	Negatively regulates transcription of bacterial ribonucleotide reductase nrd genes and operons by binding to NrdR- boxes
GWK75_RS03015	COG1328	CTP reductase activity
GWK75_RS03060	COG0336	Belongs to the RNA methyltransferase TrmD family
GWK75_RS03070	COG1837	Belongs to the UPF0109 family
GWK75_RS03085	COG0571	ribonuclease III activity
GWK75_RS03095	COG0781	Involved in transcription antitermination. Required for transcription of ribosomal RNA (rRNA) genes. Binds specifically to the boxA antiterminator sequence of the ribosomal RNA (rrn) operons
GWK75_RS03105	COG0495	leucyl-tRNA aminoacylation
GWK75_RS03115	COG0317	In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance
GWK75_RS03130	COG0765	amino acid transport
GWK75_RS03135	COG0834	ABC-type amino acid transport signal transduction systems periplasmic component domain
GWK75_RS03145	COG1619	proteins homologs of microcin C7 resistance protein MccF
GWK75_RS03260	COG1131	(ABC) transporter
GWK75_RS03280	COG0330	HflC and HflK could
GWK75_RS03285	COG1181	Belongs to the D-alanine--D-alanine ligase family
GWK75_RS03300	COG0366	Alpha amylase catalytic
GWK75_RS03325	COG0724	RNA recognition motif
GWK75_RS03335	COG0272	DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA
GWK75_RS03380	COG1950	PFAM Membrane protein of
GWK75_RS03385	COG0006	proline dipeptidase activity
GWK75_RS03400	COG3118	belongs to the thioredoxin family
GWK75_RS03420	COG1132	(ABC) transporter
GWK75_RS03425	COG1825	This is one of the proteins that binds to the 5S RNA in the ribosome where it forms part of the central protuberance
GWK75_RS03430	COG0586	Pfam SNARE associated Golgi protein
GWK75_RS03435	COG0178	The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by uvrB, the uvrA molecules dissociate
GWK75_RS03445	COG2925	Exonuclease
GWK75_RS03460	COG0536	An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control
GWK75_RS03465	COG3942	CHAP domain
GWK75_RS03465	COG1388	LysM domain
GWK75_RS03475	COG0816	Could be a nuclease involved in processing of the 5'-end of pre-16S rRNA
GWK75_RS03485	COG0849	cell division
GWK75_RS03495	COG0013	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain
GWK75_RS03530	COG0084	hydrolase, TatD family'
GWK75_RS03535	COG0143	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation
GWK75_RS03570	COG0149	triose-phosphate isomerase activity
GWK75_RS03575	COG0126	phosphoglycerate kinase activity
GWK75_RS03580	COG0469	pyruvate kinase activity
GWK75_RS03590	COG1136	(ABC) transporter
GWK75_RS03655	COG0424	maF-like protein
GWK75_RS03670	COG0456	acetyltransferase
GWK75_RS03670	COG2320	Catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A
GWK75_RS03675	COG5002	protein histidine kinase activity
GWK75_RS03680	COG0745	RESPONSE REGULATOR receiver
GWK75_RS03710	COG0112	Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF- independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism
GWK75_RS03715	COG0112	Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF- independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism
GWK75_RS03740	COG5002	protein histidine kinase activity
GWK75_RS03750	COG2205	protein histidine kinase activity
GWK75_RS03750	COG0784	Response regulator, receiver
GWK75_RS03755	COG0190	Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate
GWK75_RS03775	COG0812	UDP-N-acetylmuramate dehydrogenase activity
GWK75_RS03805	COG0766	Cell wall formation. Adds enolpyruvyl to UDP-N- acetylglucosamine
GWK75_RS03845	COG0542	response to heat
GWK75_RS03855	COG0770	UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity
GWK75_RS03885	COG0588	phosphoglycerate mutase activity
GWK75_RS03890	COG0148	phosphopyruvate hydratase activity
GWK75_RS03900	COG1051	belongs to the nudix hydrolase family
GWK75_RS03910	COG1214	PFAM Peptidase M22, glycoprotease
GWK75_RS03915	COG0802	atpase or kinase
GWK75_RS03920	COG2804	Type II secretory pathway, ATPase PulE Tfp pilus assembly pathway, ATPase PilB
GWK75_RS03955	COG2137	regulation of response to DNA damage stimulus
GWK75_RS03975	COG4968	Prepilin-type N-terminal cleavage methylation domain
GWK75_RS03985	COG2255	four-way junction helicase activity
GWK75_RS03995	COG0632	four-way junction helicase activity
GWK75_RS04000	COG0744	Peptidoglycan polymerase that catalyzes glycan chain elongation from lipid-linked precursors
GWK75_RS04005	COG0817	Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group
GWK75_RS04020	COG0217	transcriptional regulatory protein
GWK75_RS04045	COG0189	ligase activity
GWK75_RS04075	COG1253	flavin adenine dinucleotide binding
GWK75_RS04085	COG0443	Heat shock 70 kDa protein
GWK75_RS04090	COG0448	Catalyzes the synthesis of ADP-glucose, a sugar donor used in elongation reactions on alpha-glucans
GWK75_RS04100	COG0494	nUDIX hydrolase
GWK75_RS04110	COG1420	regulation of RNA biosynthetic process
GWK75_RS04120	COG0194	GDP metabolic process
GWK75_RS04160	COG0481	GTPase activity
GWK75_RS04175	COG0481	GTPase activity
GWK75_RS04185	COG0465	Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins
GWK75_RS04190	COG0037	tRNA processing
GWK75_RS04200	COG0389	Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII
GWK75_RS04240	COG1249	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family
GWK75_RS04285	COG0773	UDP-N-acetylmuramate-L-alanine ligase activity
GWK75_RS04290	COG0530	calcium, potassium:sodium antiporter activity
GWK75_RS04300	COG0220	tRNA (guanine-N7-)-methyltransferase activity
GWK75_RS04305	COG0668	transmembrane transport
GWK75_RS04320	COG0173	Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp Asn)
GWK75_RS04330	COG1651	Required for disulfide bond formation in some periplasmic proteins. Acts by transferring its disulfide bond to other proteins and is reduced in the process
GWK75_RS04330	COG3004	Na( ) H( ) antiporter that extrudes sodium in exchange for external protons
GWK75_RS04335	COG0477	Major facilitator Superfamily
GWK75_RS04360	COG1695	Transcriptional regulator
GWK75_RS04365	COG1136	(ABC) transporter
GWK75_RS04370	COG0577	efflux transmembrane transporter activity
GWK75_RS04370	COG1136	(ABC) transporter
GWK75_RS04385	COG1872	DUF167
GWK75_RS04390	COG3620	sequence-specific DNA binding
GWK75_RS04395	COG0610	Subunit R is required for both nuclease and ATPase activities, but not for modification
GWK75_RS04415	COG0732	type I restriction modification DNA specificity domain
GWK75_RS04430	COG0286	site-specific DNA-methyltransferase (adenine-specific) activity
GWK75_RS04435	COG2003	Belongs to the UPF0758 family
GWK75_RS04440	COG5002	protein histidine kinase activity
GWK75_RS04440	COG3850	Histidine kinase
GWK75_RS04450	COG1619	proteins homologs of microcin C7 resistance protein MccF
GWK75_RS04490	COG0701	Predicted permease
GWK75_RS04495	COG0494	nUDIX hydrolase
GWK75_RS04565	COG1215	transferase activity, transferring glycosyl groups
GWK75_RS04585	COG0463	Glycosyl transferase, family 2
GWK75_RS04645	COG1089	Catalyzes the conversion of GDP-D-mannose to GDP-4- dehydro-6-deoxy-D-mannose
GWK75_RS04650	COG0045	succinate-CoA ligase (ADP-forming) activity
GWK75_RS04655	COG0074	Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit
GWK75_RS04685	COG0706	membrane insertase activity
GWK75_RS04695	COG0230	Belongs to the bacterial ribosomal protein bL34 family