LOCUS NC_038279 12790 bp RNA linear VRL 24-AUG-2018 DEFINITION Drosophila ananassae sigmavirus nucleocapsid protein (N), polymerase-associated protein (P), PP3 (X), matrix protein (M), glycoprotein (G), and RNA-dependent RNA polymerase (L) genes, complete cds. ACCESSION NC_038279 VERSION NC_038279.1 DBLINK BioProject: PRJNA485481 KEYWORDS RefSeq. SOURCE Drosophila ananassae sigmavirus ORGANISM Drosophila ananassae sigmavirus Viruses; ssRNA viruses; ssRNA negative-strand viruses; Mononegavirales; Rhabdoviridae; Sigmavirus. REFERENCE 1 (bases 1 to 12790) AUTHORS Longdon,B., Murray,G.G.R., Palmer,W.J., Day,J.P., Parker,D.J., Welch,J.J., Obbard,D.J. and Jiggins,F.M. TITLE The evolution, diversity and host associations of rhabdoviruses JOURNAL Virus Evol (2016) In press REMARK Publication Status: Available-Online prior to print REFERENCE 2 (bases 1 to 12790) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (24-AUG-2018) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 3 (bases 1 to 12790) AUTHORS Longdon,B. TITLE Direct Submission JOURNAL Submitted (14-MAY-2015) Genetics, University of Cambridge, Downing St, Cambridge, Cambs Cb2 3EH, UK COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to KR822812. ##Assembly-Data-START## Assembly Method :: Trinity v. 2013-02-25 Sequencing Technology :: Illumina ##Assembly-Data-END## COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..12790 /organism="Drosophila ananassae sigmavirus" /mol_type="genomic RNA" /isolate="Kilifi Kenya" /host="Drosophila ananassae" /db_xref="taxon:1002359" /country="Kenya" /collection_date="2010" /collected_by="Ben Longdon" gene 109..1536 /gene="N" /locus_tag="D1P07_gp1" /db_xref="GeneID:37616327" CDS 109..1536 /gene="N" /locus_tag="D1P07_gp1" /codon_start=1 /product="nucleocapsid protein" /protein_id="YP_009505486.1" /db_xref="GeneID:37616327" /translation="MSAPMEEDHIPGSSNQNRQQPITKGEVTSSSIYKVTKEKVEFSS EPTAAEAVYPSAWFNNTKTKPTLIIPQCPPGQVYSVEVLRLNVVEHLLSGTLTKDIAM LYVYTELSRIEGTLSEDWTSFGVRIGLRDEKVTPFSPVTVKQEGNVVHLSPKSGECQY AYWALASILSVYRLSRTSYADYRSRMSESLKGQSEIVRPATALGYDEMSITYGAWKDD QNFRKMIATIDMFYVKFPGLQFDLIRFGTVSSRYKDCAALTSLFNLKHTMGVSKESQI SLWIGNEVIANQFIRITKPGEEIDKVNSYFPYLIDFGLTIKSPYSSVLNANFYFFANT AAILMGNTRARNAVYHEIDSSSSTLMNSVLLGYAHAGRSMNSQMFFQSEEDLKLHEEN SEMREALKATQIGVRVATDKEPEDRDASDWFDYMDKINWTYTDKMKLFNQSIKRTLAD CRDGSLGNKLKALLPVPENSDRESL" gene 1637..2617 /gene="P" /locus_tag="D1P07_gp2" /db_xref="GeneID:37616322" CDS 1637..2617 /gene="P" /locus_tag="D1P07_gp2" /codon_start=1 /product="polymerase-associated protein" /protein_id="YP_009505487.1" /db_xref="GeneID:37616322" /translation="MNTKKILRESKLEDNLGHKTKKPKLANALTRGLIPEENRRQEGD IPMSVIDESIQDEKMTEDIDHKPKKSSNIIQAPSLGHRWLDSYTRSEPDIATLHDDGS VRGVPIERPPVITQPVDDEMEETRQGEEFLMSLQQDHQTLHFENESATIAEEEGEAYI MFGKYVTKECQVTTLRILEVVDQYCKSKGIFIPRLLVKKVESDPSAVSLSPHFSPDSP KEPKKSTIGGTQRQPSQPDRPGTPVPDSNSVAMVRCIKEGVYLKSKSEGGPRHKIDLS SLAMSEEEIVSLYHQMKPANLKEFVKYCLVFRGQFKIIARRYDLSSVSTQ" gene 2648..3700 /gene="X" /locus_tag="D1P07_gp3" /db_xref="GeneID:37616323" CDS 2648..3700 /gene="X" /locus_tag="D1P07_gp3" /codon_start=1 /product="PP3" /protein_id="YP_009505488.1" /db_xref="GeneID:37616323" /translation="MLNKKNQTKINPQNLDSHPSPKMQITKLNLIVLFTSTYWTITIA IGPICRCEGDIELDKVGSINMTTTYHVPYGHIVKMEQTLIFETKSDNGSIKRLVQSCE KSNEETGIMSMYSQDEIAKQKTGMLAQMEIPESDEIDDSPDSPLKVSLLSPEHPLSSN SIPKTSEEVFNDPGTLTVHHVGRAIHTPGKSPRAPEDPTKKIPEWSLNLGNGYRQRTK RAIDNGVTSRFKPVSEMMTSEYLSKYDFLLSRRIKEEEKIWVDIADLIEIWVGSIVNP IHVSCNKKGHIRMILKCISIHMKSIFDSVHYPTIMGLLGEILILDVKIRNDFAAWSGI RTLRQDQENSRRRGRG" gene 3802..4494 /gene="M" /locus_tag="D1P07_gp4" /db_xref="GeneID:37616324" CDS 3802..4494 /gene="M" /locus_tag="D1P07_gp4" /codon_start=1 /product="matrix protein" /protein_id="YP_009505489.1" /db_xref="GeneID:37616324" /translation="MLAKIGEIISSGKRRKEQQISNYLLAGSSKLAEMDGRISERPNP VASAPNYSDLIRNDPHQDYQKSKYLITGELELITSEDLRTYTDLFDKLNIWVDDYIGD PGLRPIYSLVYVLIGTHLMKRKMSSDSRFVYSGHVTQLTCINHLFGTIHSERKDFSWN SVSGLGESKCIIKFDCTLKPTNRTCVNFMDVYLRTMPDSDPPPFINHILEEYGIELVQ KSESDTYFKYKV" gene 4564..6129 /gene="G" /locus_tag="D1P07_gp5" /db_xref="GeneID:37616325" CDS 4564..6129 /gene="G" /locus_tag="D1P07_gp5" /codon_start=1 /product="glycoprotein" /protein_id="YP_009505490.1" /db_xref="GeneID:37616325" /translation="MEIVIPILNLTAWSIVDPFNLTCPQMLDYNHSPSDISVPITIKY PAHNEGKRKVPGFLCSCFSLTTRCTEAWTWSTEVSYHSSTIECDLMSCKAEIEKFKAG KLDVKMYPREDCVYARTNEVEAKYIQITPHSSFMDPYNGYICDEIFLTGRTPGNKSET IYSGTLWVLDDGWAKESLCEDWTEISGKLILPNETVEQDLLHSKGTVWAHEMPIHKLS EACSIKYCGTVGLVFPDGMWMNIEMQRDIDSRLRMFLYGGKTCEKDQMIKLPTSNHDL VYTEMNTLNAMYLMKCHECISKIRMGAQVTNYELSFLSRSYPGIGPIYRISEHGIIQA VGLYKKIKTDSRNGKQNILGLDKDGSSVTFKDWVTIRNVTYGIGGTIKDRNGEIIFPS DIVTRTQVSSELMMSTHLHQIYHPLDDHLRRRLNLSTPIRLRHDGSTNVIQEAGKAIL KTGNEFTAWISKTWEGFRMYVYSVIGAILILIYTKFKKTINRDKTQGDESQGLPLSVN PRREAQVPMQWRT" gene 6281..12682 /gene="L" /locus_tag="D1P07_gp6" /db_xref="GeneID:37616326" CDS 6281..12682 /gene="L" /locus_tag="D1P07_gp6" /codon_start=1 /product="RNA-dependent RNA polymerase" /protein_id="YP_009505491.1" /db_xref="GeneID:37616326" /translation="MEEDSFSQFLTDPMLDDEQFNLFEDSDIPSVPGSSITGLSNNDY SLNSPLVADEMVEYVRYVKYGTTSKRWSMKRWESRRTIMERHVNLDKLLFADSFHKFF AEFNLYHPEDSTEFKELLTESINDSKDTMEIPNAFIRGWIGPEFVPRKEVKFTKEILR WGSFFWELHQVILCLNAKTHFEATNLSKWIKMKLVWCEERKGMSVDLKNFGLVLMSEG FVYFKNHNILLDRNCVLMMKDTYAARFHTLLAMSSRVDKKFTNLDIDKMNEIYKLGDE LLAREGSDGYKGLKLIEPICNLRLTELARKYRDKIPRFPKFKVHVTNKVRALGTRFHP ISTMSRLIMKEDSVELVLTIYGSFRHWGHPMIDYKAGLEALYSQVNAKKIIDRIYAEQ LASDLALRVLKYKFHQTKTWYVDVDLMDDSPMKDMMRQNLYPTPAMISDFGDNWHKLP LKKCFNIPEMIDPSLIYSDKSHSIQYNELVDHLQGPNRNLPVPSKKVLTTLLEREATN WPEFLERVDKVGLPSNSLLIGLKLKEREQKDKGRFFSLMSWELRDYFVFTEYLIKTHF VPLFEGLTMADDLTTVTQKMLSSSSGQGNDDYNTITIANHIDYEKWNNHQRGDANGPV FKVMGQFLGYPNLIARTHEFFEKSLIYYSDRADLMMVRDGKVVNMSDFLVCWDGQKGG LEGLRQKGWSIVNLLVIEREGKSRNTTVQTLAQGDNQVICMKYKPRTSSGDRDLIKNL QEIVQNNNNLMSNIEGGTKRLGLIINNDETMQSADYLNYGKVPVFRGNIRGLETKRWS RVTCATNDQLPSLGNIMATVTSNALTVSHHSTSCINSIYHMNLLGSFVRNIIMKHNPS IQSAPLFDPDSGLTVKSRTFKILSVYLDPSLGGVSGTSLTRFLIRAFPDPITEALTFW RIIHNNTVDNDIKLLCAKVGNPRTVPFQSRHISKLAEDPQSLNIPRGISSKTMIKEEI KYALLLNRSKIKNEVVSSILDHIQRDELSLWTLLESIKPRFPRFISELRASTFSGLAN EIVGLFQNSRTIRNQFKHKFSERVNKVIMQSELLSIKTLCKYDSERHQFQMWSCSSQK ADNLRVRSWGDTVVGATIPHPMEMTTSGHLLTEPCNLCNLGNLRNQYIAVHLPEGLTN YTDKRGPCAPYLGSRTSESTSILTPWERESKVPLIKRAMKLRNSIGWFIDPDSNLAKT ICDNLTALTGEDWSGKVTNYRRTGSALHRFSSSRQNTGGYAAQSPAKLTWINSTTNTL TDIGDQNYDFIFQSLLIYSQVTVGELHDGIPAQGCYHFHLTCLDCLRPIEEPTLETEM KYKFQDMSKLLEKWKPENVPWSHTRDPVYLPQGDWSAVANSRKSYHIGRAEGFVFGTG SLSKTDHIERANLFPLSFRGKVCPINYMEGLLDGLVRSSAIEVINRRQVIHCLKPQSA LMGSILNLIRVIGTSPSLITLWRTDDFLDLFYQSRHKVPDSYPLKDVDLTSLGTSYLR ELLANSKQYITWGNYKPERIWIFADLDKKKWIGLFGISTKLLPILCNPVVKSTSFQLF RDMKSLTSSILTMDFTYIEPPIDNVIGDLVGLDREVRHALKCDSYDFIKENLRARTIG RGQWGREYICKVQAYDVHYDNGQLTDNPTPIPNVPKFTNPSISGFKTFQCSSNAHFKL RGIIHHIHFKPNDIICGADGSGGMSAMLLREFSTSRLIFNSFLKMEGVSLRGSNPSPP SAIHECIEVRDRCINLMDVWQNPSDLALGVTWTYFRRLIHRFNLKVNLMVFDMEYQSE EITRKIEENLILNLDIMKDRNCCVIYKTYLGILYNQQENVLTRIGPLFKSVQVMHTEL TSSQSSEVYVVMQFLKRDNIFRRNVNLAKLISDTMISWPVFQSIDSEIDRAQSVMRMD LMMGVPRSLRIDPFIELGSVLRSLGVQSGTTFALLGVGDKHSEPIPPYQPILVYLVSM NSIFDVIMESSHPLNIPSDETVLNIGSLMIGFMLWYGHQFDLRYEIARCLSILNNNFP FNWGCLETSGGRFRQVSSLCNKMRDRKYLNMASKIGLISHLMRLFHLAYGNMEMLNDT KTLDNALKKFNAGFNCKRLGVRNGVISDIHSLSPIITEEASPGFEIMDPTGLEEVVWR S" ORIGIN 1 ccgccagttc ttctatagac caacaggcat ataaatgtta aaattcaaca caactttaaa 61 taaacagtga gtcagtgtgt aatactaatc tattaaaacg gaaatatcat gagtgcccca 121 atggaagagg accatattcc tggatcatcc aatcaaaacc gacaacaacc aatcacaaag 181 ggagaagtta cgtcatcctc catttacaaa gtgaccaagg aaaaagttga gttctcttct 241 gaaccaaccg ctgctgaggc agtttatcca tcagcatggt ttaacaatac caagactaaa 301 cccactctca tcatccctca atgcccaccc ggtcaggttt attctgtaga agtactccga 361 ctaaatgttg ttgaacatct cttgtcagga acattgacca aagatattgc aatgttatat 421 gtttacactg aactatctcg tatagaggga actctatctg aggactggac ttcatttgga 481 gtaagaattg ggttaaggga tgagaaagtt acaccattta gtcctgtaac cgtcaaacaa 541 gaaggaaatg tagtccacct atctcccaag agtggagaat gccagtatgc ttattgggct 601 ttagcctcta tcttgtctgt ttacagatta agcagaactt catatgcaga ctacagatcc 661 agaatgtcag aatctctcaa gggacagagt gaaattgtgc gtcctgcaac agcccttggg 721 tatgatgaaa tgtcaataac atatggagca tggaaggatg atcaaaactt cagaaagatg 781 attgccacaa ttgatatgtt ttatgtgaag ttccctggtc tccaatttga cttgataaga 841 tttgggactg ttagctctcg gtacaaggac tgtgccgcat tgacatctct attcaactta 901 aagcacacta tgggtgtttc taaggagagt caaatatctc tatggattgg gaatgaagta 961 attgcaaacc aatttatccg aattacaaag ccaggtgaag aaatagataa ggtaaactca 1021 tatttccctt acttaattga ttttggattg acaatcaaat ccccttattc atcagtctta 1081 aacgccaatt tctatttctt tgcaaacact gccgcaatac ttatggggaa caccagagca 1141 agaaatgctg tgtatcatga gattgattcg tctagctcaa ccctgatgaa tagtgtttta 1201 ctgggttatg ctcacgcagg tcgttctatg aactcccaaa tgttcttcca atcagaagaa 1261 gatctcaagc ttcatgagga aaactcagaa atgagggagg cactaaaagc cacccaaatt 1321 ggagtacggg ttgctacgga caaagagccc gaggaccgag atgcaagtga ttggtttgat 1381 tatatggaca agatcaattg gacttacacc gacaaaatga agttattcaa tcagtctatc 1441 aagagaactc ttgcagattg tagagatgga tcactgggaa acaagttgaa agcactgctc 1501 cctgttcctg aaaactccga tagagagtct ttatgagacc aaattcaagg ggtcaataac 1561 ttgagatcca ggatgcattt tgataaacta aacatgaaaa aaatggaaca ataaaactta 1621 acacaaatca atcataatga acacaaagaa aatcttaagg gagagcaaac tggaggacaa 1681 tctaggtcac aagaccaaga agccgaagct ggccaatgcc ttgactagag gattaattcc 1741 ggaggagaac aggagacaag agggtgatat tccgatgtca gtaattgatg agtctatcca 1801 agatgagaaa atgaccgagg acattgacca caaaccaaag aaaagcagta atatcatcca 1861 agctccctca cttggacata gatggcttga cagttatacc agaagtgagc ctgatattgc 1921 cacacttcat gacgatggat cagttcgagg agttcccata gagagacctc ctgtaattac 1981 tcaacccgta gacgatgaaa tggaggaaac aaggcaaggg gaagaattcc taatgagcct 2041 tcagcaggat catcaaacac tccactttga gaatgagtcc gcaactatcg cagaagagga 2101 gggggaggct tatataatgt ttggaaaata tgttactaaa gaatgccagg ttacaacact 2161 ccggatcttg gaagtggtgg atcagtactg caagtccaag ggtatcttca ttccccggct 2221 actggtaaag aaggttgagt cagatccctc ggctgtttcc ctttctcctc acttcagccc 2281 tgattcacca aaggaaccaa aaaagagtac aataggagga actcagagac aacccagtca 2341 accagacagg ccgggtactc cagttcctga ttctaactca gttgcaatgg tccgttgcat 2401 taaagagggc gtctacttga aaagcaagtc agagggagga cctagacata aaattgatct 2461 atcgtccctt gcaatgtctg aagaggaaat agtgtcacta tatcatcaga tgaagcctgc 2521 aaatttgaaa gagtttgtaa agtactgtct cgtatttcgt ggtcaattta agataattgc 2581 ccgtcgttat gacttatctt cagtttccac tcaataaatg acatgaaaaa aatggagtta 2641 aacaaaaatg ctcaacaaaa aaaatcaaac taagataaat ccacaaaacc ttgattctca 2701 ccctagtcca aaaatgcaaa taactaaatt aaatctgatt gttttgttca catcaaccta 2761 ttggacaatc accattgcaa ttggtccaat ttgtagatgt gagggtgaca tagaacttga 2821 taaagtcggt tcaatcaaca tgacgaccac atatcatgtt ccttatggtc atattgttaa 2881 aatggaacag accttgatat tcgaaacgaa atctgacaac gggtccatca aacgattggt 2941 ccaatcatgt gagaaatcaa atgaggaaac ggggataatg tctatgtact cccaggatga 3001 gatagccaaa cagaaaactg gtatgttagc tcagatggaa attccagaat cagacgaaat 3061 tgatgactcc ccagatagtc ctctaaaagt cagtttgcta tctcctgagc acccattaag 3121 ttctaattca atccctaaaa catcagaaga ggtttttaat gatccaggaa ctttaacagt 3181 tcaccatgta gggagagcaa tccatacccc tggcaaatct cctcgtgctc cagaggaccc 3241 cactaagaaa attcctgaat ggtctcttaa ccttggaaat ggttataggc agagaactaa 3301 aagagcaatt gacaacggag tcacatctag gttcaagcca gtgtctgaaa tgatgacttc 3361 tgagtatttg tccaagtatg acttcttact cagcagaaga attaaggaag aagaaaagat 3421 ctgggttgat atcgcggacc taattgagat atgggtaggg tctatcgtaa atcccataca 3481 cgtaagttgt aataagaagg ggcatatcag gatgatatta aagtgtattt caattcatat 3541 gaagtcaata tttgattctg tacactatcc aaccatcatg ggtttactag gtgaaatctt 3601 gatattagat gttaaaatca ggaatgattt cgcagcttgg tcagggatta gaactctcag 3661 acaagatcag gagaattcta gaaggagagg aagagggtga gatccgagaa agttaacttg 3721 atagaaattt cagtgagtga ttatgcatga aaaaaacaga caggtaaaca gcaacaggaa 3781 atcaaatcgg tcagaatcaa aatgcttgcg aaaataggag agatcatctc gagcgggaag 3841 agaaggaagg agcaacaaat atcaaattac cttctagcag gcagctcaaa attagcagag 3901 atggacggta gaatttcaga acgaccaaat cctgtagcat cggcaccaaa ttattcggac 3961 ttaatacgta acgacccaca ccaagattac caaaaatcaa aataccttat aactggggaa 4021 ttggaattga taacatctga agatttacgt acttatactg atttgtttga caaattgaat 4081 atttgggtgg atgattacat tggtgatcct ggacttcgac caatttactc tctcgtttat 4141 gttttaattg gaacacactt aatgaagagg aaaatgtctt cagattcacg gtttgtttat 4201 tctggtcatg tcactcaact aacctgtata aatcatctat ttgggactat tcattctgaa 4261 agaaaagatt tctcatggaa tagtgtctct ggactgggcg aatctaaatg tattataaaa 4321 tttgattgca cattgaaacc tactaacaga acatgtgtaa atttcatgga tgtttacctt 4381 cggacaatgc ccgattcaga tcctccacca ttcattaacc acatcttaga agaatatgga 4441 attgaattgg ttcaaaagtc cgagtccgac acttacttta agtataaagt ttaatcaaga 4501 taatttgtct tctcttatga acatcactac atgaaaaaaa cattcaacaa acatcaatac 4561 aaaatggaaa tcgttattcc cattctgaat ctcactgctt ggtccattgt ggacccgttt 4621 aatctaactt gtcctcaaat gctagactat aatcattctc cgagtgatat atcagtaccg 4681 attaccataa agtatccagc ccataatgaa ggcaaaagga aggttccagg attcttgtgt 4741 agttgtttca gtctgacaac ccgctgtacc gaagcatgga cctggtctac cgaggtctca 4801 tatcattctt caaccatcga gtgtgattta atgagctgta aagctgaaat tgagaagttc 4861 aaagcaggga agctagatgt caagatgtac ccacgggaag actgtgtgta tgcaaggact 4921 aatgaagtag aagccaaata catccaaatc acacctcata gcagtttcat ggacccctat 4981 aatggataca tctgcgacga aatattcctg acaggcagga cccctggcaa caaatctgaa 5041 accatctata gtgggacact ttgggtccta gatgatggct gggcaaagga atctctgtgt 5101 gaggattgga cggagatatc aggcaaactg attctgccaa atgagactgt agagcaagac 5161 ttgttacatt ccaaagggac ggtgtgggct catgagatgc caattcataa actgtcagag 5221 gcgtgttcaa ttaaatattg tgggactgta ggtcttgttt tccctgacgg aatgtggatg 5281 aatatcgaga tgcaacgaga tattgattca cgtctaagga tgtttctata tggtggtaaa 5341 acatgtgaga aggatcaaat gataaaattg cccacatcaa atcatgattt agtatacacc 5401 gagatgaata cattaaatgc tatgtattta atgaagtgtc atgaatgcat atctaagatc 5461 cgaatggggg ctcaagttac aaattatgaa ttatcattcc tttcgcggtc atacccagga 5521 atcgggccca tctacaggat aagtgaacat ggaattatcc aagcagtggg tctgtacaag 5581 aagatcaaga ctgacagtag aaatggaaaa caaaacatac taggattaga taaagatggg 5641 tcctctgtaa cattcaaaga ttgggttact ataagaaatg ttacctatgg aatcggaggt 5701 acaatcaagg ataggaatgg agaaattata tttcctagtg acattgtgac ccgaactcag 5761 gttagctctg aattaatgat gagcacacat cttcatcaga tctatcaccc tcttgatgat 5821 catttgagaa ggagactgaa cttgtccact cctataagac tgagacatga tggatcaacg 5881 aacgtaattc aggaggcagg gaaagcaatc ttaaaaacag gcaatgagtt cactgcatgg 5941 atcagcaaaa catgggaagg attcagaatg tatgtatact ctgtaatagg ggctattctc 6001 atacttatct acacaaaatt taaaaagaca atcaaccgag acaagaccca aggggacgaa 6061 tcacaaggac tgcctctgtc agtaaatcct agaagggaag cccaggttcc gatgcaatgg 6121 agaacatgaa aaaaactaac aacttcaatg aaaaatcaac acatataacc acatatcact 6181 ctagaagtgc accgataaac tacttgtatt ctttcaacaa ataacattaa gctataagac 6241 acaatctgcc tactaattat cataaatacc caaccaggcc atggaggaag attcattttc 6301 tcagttccta acagacccta tgctggatga tgaacagttc aacttatttg aagactcgga 6361 tattccgtct gtaccaggat cttcaattac cggactatcc aacaatgact attcactaaa 6421 ttccccacta gttgccgatg agatggtaga gtatgttagg tatgtgaagt atgggacaac 6481 ctcaaaaagg tggagtatga aaaggtggga aagtagacgt acaatcatgg agagacatgt 6541 aaatttagat aagttgctgt tcgcggattc attccataaa ttcttcgcag agttcaattt 6601 ataccaccca gaagattcta ctgaatttaa ggaactcctg actgagtcta tcaatgactc 6661 taaggacaca atggagatcc ccaatgcatt cattagagga tggataggcc ctgaatttgt 6721 tccgaggaaa gaagttaaat tcaccaagga aatcctaaga tggggatcgt tcttctggga 6781 attacatcaa gtgattcttt gtttaaatgc aaagactcat tttgaagcca caaacttgag 6841 caaatggata aagatgaagt tggtctggtg tgaagaacgg aaaggaatgt ctgtcgattt 6901 gaaaaacttt ggtctggttc taatgtcaga aggctttgta tattttaaaa accacaacat 6961 actgctagat agaaattgtg tcctaatgat gaaggacaca tacgccgcta gatttcatac 7021 attgttagct atgagttcaa gagtggataa gaagtttaca aacctggata tagacaagat 7081 gaatgaaatc tacaagctcg gagatgaact cttagcaaga gaaggatccg atggctacaa 7141 gggattgaaa cttatagagc ctatttgtaa cctaaggcta acggagttag caaggaagta 7201 cagagataaa attcctaggt tcccgaaatt taaagttcat gttactaaca aagtcagggc 7261 actaggtacc aggtttcatc caattagcac catgagtaga ctcatcatga aggaagattc 7321 agttgagctt gtcttaacta tctatgggtc atttcggcat tggggacacc ccatgataga 7381 ttacaaagca ggattggagg ctttgtatag tcaagttaat gcaaaaaaga ttattgatag 7441 aatctatgct gaacaattag cgagtgacct tgcactaaga gtactaaaat ataagtttca 7501 ccaaacgaag acttggtatg tagatgtcga cttaatggat gattccccga tgaaagacat 7561 gatgagacaa aacctatacc cgacaccggc aatgatatct gatttcggag acaactggca 7621 caaactacca ttgaagaaat gtttcaacat ccctgaaatg attgatcctt cactgatata 7681 ctctgataag agtcattcaa tccagtacaa tgaattggta gatcacttac agggaccaaa 7741 cagaaacctg cctgttccat ctaaaaaggt tttaaccact ttgttggagc gagaggcaac 7801 caactggcca gaatttcttg agagggtgga taaagtagga ctaccatcaa attctttact 7861 aattggactt aaattgaaag agagagaaca gaaggataaa ggaaggttct tttcactcat 7921 gtcttgggaa ttgagggact acttcgtgtt taccgaatat ctaattaaaa cacacttcgt 7981 tccattattt gaaggcctga caatggcaga tgatctaact acagttactc agaagatgct 8041 gagcagctcc tctggacagg ggaatgatga ttataataca ataactattg ccaatcacat 8101 agattatgag aaatggaata atcatcagag aggtgatgca aatggcccag tattcaaagt 8161 gatgggccaa tttctagggt atcctaatct aatagcacga acccatgaat tcttcgaaaa 8221 gagcctgatc tattacagtg atagagcaga tttgatgatg gtcagagatg gtaaggtggt 8281 aaacatgtcc gatttcctgg tatgttggga tggacaaaag ggaggccttg aaggacttcg 8341 acaaaaagga tggagcattg tcaacttgct agtaattgag agagaaggga aaagcaggaa 8401 tactactgtc caaacacttg cacaggggga caatcaagtt atatgtatga aatataagcc 8461 acgcacctca tcaggggaca gggatttaat caagaaccta caagaaattg ttcaaaataa 8521 caacaatcta atgtctaata ttgaaggagg aacaaaaaga ttggggttaa taataaataa 8581 tgacgaaact atgcagtcag ctgattatct aaattatgga aaagttcctg tcttcagagg 8641 gaatattaga ggactagaga ccaaacgatg gtcaagggtc acctgtgcaa caaatgatca 8701 actcccatcc ctagggaata ttatggccac tgtgacaagt aacgcactga cggtatccca 8761 ccacagcaca tcttgtatca actcaatata tcatatgaat ttactaggat ccttcgtccg 8821 aaacattata atgaaacaca atccatcaat acaatctgcc cccttgtttg atcccgacag 8881 cggattgacc gtcaaatcaa ggaccttcaa aatcttgagt gtctatctcg atccctccct 8941 tgggggagtg agtggtacct ccctaactag attcctaata agagcattcc cggatcctat 9001 aacagaagct ctgacattct ggcggataat ccataacaac acagttgata atgacataaa 9061 gttgctttgt gccaaagtgg gaaacccaag gacagtccca ttccaatcca gacatatatc 9121 aaaactggcg gaagatcccc aatctctaaa catccctagg gggatcagct caaagacaat 9181 gataaaagaa gaaataaagt atgcgttgtt attgaataga tcgaaaatta aaaatgaggt 9241 ggtgagcagc atattagatc acatccagag ggatgaactg agcctatgga ctcttttaga 9301 atctataaaa ccaagattcc ctcgctttat cagtgaatta agggcaagca ccttctctgg 9361 attagccaat gaaatcgtcg gtttattcca aaattctagg acaattagaa atcaatttaa 9421 gcacaagttt tcagagaggg tcaataaagt tattatgcag tctgaacttt tatcaataaa 9481 gactctatgt aagtatgact ctgagaggca tcaattccaa atgtggagct gctcttcaca 9541 gaaagcagat aatctgcgag tcagatcatg gggagatacc gtagtaggag cgacaatccc 9601 acacccaatg gaaatgacaa catctggaca tttactcacc gagccctgta atctatgtaa 9661 tttgggcaat ctaagaaatc agtacattgc agttcactta cctgaggggt tgactaatta 9721 tactgacaaa agagggccat gtgcgcctta cctggggtct cggacttcag aatctacaag 9781 cattctaacc ccctgggaaa gagaatctaa agtcccccta ataaagagag caatgaaatt 9841 aagaaactca ataggctggt tcattgaccc agatagcaat cttgccaaga caatatgtga 9901 caacctgaca gctttaacag gggaggattg gagtggaaag gtaactaatt acagaaggac 9961 aggctcagca ttacatcgat tctcatcatc ccggcagaat acgggaggtt atgcagctca 10021 gagtccggcc aagttgacct ggattaactc aacaactaac actcttactg acattggcga 10081 ccaaaattat gatttcattt tccagtcatt attaatttac tctcaggtta cggtgggaga 10141 gttacatgat ggtattccgg ctcaaggctg ttatcatttc cacttgacat gtttggattg 10201 tctgagacca attgaagaac caacactgga gactgagatg aagtacaaat ttcaagacat 10261 gtcgaaatta ctggagaaat ggaagcctga gaatgtccct tggagtcaca caagggatcc 10321 tgtatacctc cctcaggggg actggagtgc cgtggcaaac agtcgaaagt cctatcatat 10381 aggaagggcg gaaggatttg tctttggaac aggatcatta tctaagactg atcatataga 10441 acgagcgaac ctgttcccat tatcattcag agggaaagtt tgtcccatta attatatgga 10501 aggattgctt gatggactgg tgaggtcaag tgcaattgaa gtcatcaaca gaaggcaagt 10561 aattcactgt ctcaagcctc agtcagctct aatgggatct attttaaacc tcataagagt 10621 aattggaaca tctccctctc taataacact atggaggaca gatgactttc tagatctatt 10681 ttatcagtcc cgacataagg taccggattc ttatccacta aaagatgttg accttacctc 10741 gctaggaact tcttacttga gagaactact ggccaattcc aaacaatata ttacgtgggg 10801 aaattataaa ccagaaagga tctggatatt cgcagatctg gacaagaaga aatggattgg 10861 gctatttgga atatctacga aactgctacc aatactatgc aatccggttg tgaagtcgac 10921 atcattccaa ttattccgtg acatgaaaag tcttacctca tctatcctca ctatggactt 10981 cacctacatt gaaccaccca ttgacaatgt tataggagac ttagtcggtc tggaccggga 11041 agtaagacat gcattgaagt gtgactccta tgattttata aaagaaaatt tgagagcgag 11101 gaccatagga agaggccaat ggggtcgtga gtatatctgc aaagtccagg catatgacgt 11161 acactatgac aatggacaac tgaccgacaa cccaactcca atccctaatg tgccgaaatt 11221 cacgaatccc tcaatatccg gatttaagac attccagtgt tcatccaatg cccactttaa 11281 attaagagga atcattcacc atattcactt caagccaaac gacattatct gtggcgcaga 11341 tggttcggga ggcatgtctg caatgctatt gagggaattt tcaacctcca gattaatatt 11401 taacagtttt ctgaaaatgg agggggtttc attgagagga tcaaatccat ctcctccttc 11461 tgccatccac gaatgtattg aagtgagaga tagatgtata aacctcatgg atgtttggca 11521 gaacccatca gatctagcac ttggggtgac ctggacttat ttccggagat taatacatag 11581 attcaacttg aaagtgaacc tgatggtatt tgatatggaa tatcagagtg aggaaatcac 11641 ccggaagata gaggagaact tgattctaaa tttggatatc atgaaggata ggaattgctg 11701 tgttatatat aagacctacc ttggtatatt gtataatcaa caagaaaatg tgctcaccag 11761 gattggaccg ttgttcaaat cagttcaagt gatgcacaca gagttgacat cctctcagtc 11821 atctgaagtg tatgtggtga tgcagttttt gaagagggac aatatcttca gaaggaatgt 11881 aaatctagct aagctgataa gtgacactat gatatcatgg cctgtcttcc aatcaataga 11941 tagtgagatc gaccgtgctc agtcagtgat gaggatggac ttaatgatgg gtgtacccag 12001 aagcctaaga attgacccct tcatagagct gggctctgtg ttgagatctc tcggggttca 12061 gagtggaact acttttgctt tacttggagt tggtgacaaa cattcagaac caattcctcc 12121 ttatcagccg attctcgtct atcttgtgtc catgaactct atttttgatg ttataatgga 12181 gtcatcccat cccctaaaca tcccttccga cgaaacagtt cttaatatag gaagcctgat 12241 gattggattt atgctttggt atggccatca atttgaccta aggtatgaaa ttgctcgatg 12301 tttatcaatt ctgaacaaca atttcccatt caactggggc tgcctagaaa catcgggagg 12361 ccggttcagg caagtttcat cattatgtaa taagatgaga gacaggaaat atttaaatat 12421 ggcaagcaaa attggactca tatctcatct tatgaggtta ttccatctgg catatggaaa 12481 tatggaaatg ttaaatgata ccaagaccct agacaatgcc ctgaagaaat tcaacgcggg 12541 atttaattgt aaaagactgg gagttaggaa tggagtcatc tcagatatac acagtctctc 12601 tccaataatc acggaagaag caagccctgg gtttgagata atggatccaa caggactgga 12661 agaagttgta tggagaagtt agttattaat tcaagaattg tcatacccgt taaaaaaact 12721 aatcatgtta aataaagcaa ttatatagat gtaatgtagt tgtaagaact ggtagtgtct 12781 gtctcggtca //