locus_tag	old_locus_tag	gene	Name	start	end	strand	product	note	type	ID	NCBI
HOU18_gp01	LL2_001		HOU18_gp01	658	1129	+	S-adenosyl-L-methionine hydrolase	Strong matches to canonical phage DB%2C InterPro and uniref for a gene encoding S-adenosyl-L-methionine hydrolase.%3B T3-like S-adenosyl-L-methionine hydrolase	gene	gene-HOU18_gp01	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp01
HOU18_gp02	LL2_002		HOU18_gp02	1142	1402	+	hypothetical protein	Some partial matches to uncharacterized phage proteins. May be conserved%2C but not highly so.	gene	gene-HOU18_gp02	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp02
HOU18_gp03	LL2_003		HOU18_gp03	1388	1605	+	putative transmembrane protein	TMHMM detected a N out C in transmembrane domain.%3B InterPro recognizes a non-cytoplasmid domain%2C a transmembrane domain and a cytoplasmic domain.%3B T3 gp0.6-like.%3B Contains a transmembrane domain.T3 gp0.6-like protein.	gene	gene-HOU18_gp03	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp03
HOU18_gp04	LL2_004		HOU18_gp04	1575	1762	+	hypothetical protein	Matches uncharacterized protein produced by T3 gene 0.65.%3B Match to T3 gene 0.6 is directly upstream.%3B T3 gp0.65-like protein	gene	gene-HOU18_gp04	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp04
HOU18_gp05	LL2_005		HOU18_gp05	1764	2889	+	protein kinase	Strong matches to T3 and T7 protein kinases.%3B InterPro and various BLAST results suggest a T7-like protein kinase.%3B T3/T7-like protein kinase	gene	gene-HOU18_gp05	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp05
HOU18_gp06	LL2_006		HOU18_gp06	2950	5614	+	RNA polymerase	Matches RNA polymerase of phages T3 and T7%2C and many other T7-like phages.%3B T3/T7-like RNA polymerase	gene	gene-HOU18_gp06	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp06
HOU18_gp07	LL2_007		HOU18_gp07	5687	5973	+	hypothetical protein	Matches uncharacterized gp1.05 of phage T3.%3B T3 gp1.05-like	gene	gene-HOU18_gp07	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp07
HOU18_gp08	LL2_008		HOU18_gp08	6053	6206	+	hypothetical protein	T7 gp1.1-like%3B T7 gp1.1-like	gene	gene-HOU18_gp08	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp08
HOU18_gp09	LL2_009		HOU18_gp09	6194	6481	+	host dGTPase inhibitor	Strong matches to phage T3/T7 host dGTPase inhibitor. Interpro results are for proteins in the same family as dGTPase inhibitors.%3B T3/T7-like host dGTPase inhibitor	gene	gene-HOU18_gp09	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp09
HOU18_gp10	LL2_010		HOU18_gp10	6563	7622	+	ATP-dependent DNA ligase		gene	gene-HOU18_gp10	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp10
HOU18_gp11	LL2_011		HOU18_gp11	7538	8025	+	HNH endonuclease	14 matches to the canonical phage database for HNH endonucleases. Interpro results are linked to descriptions of HNH endonucleases/endonucleases.%3B Multiple strong blast hits and InterPro results for endonucleases.%3B JG-RBS missing/deleted?%3B conserved HNH endounclease	gene	gene-HOU18_gp11	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp11
HOU18_gp12	LL2_012		HOU18_gp12	8118	8388	+	hypothetical protein	Matches Hypothetical genes of phages T7 and T3. Many blast hits to uncharacterized or hypothetical proteins of various podophages.%3B Blast results suggest this protein is T3 gp1.6-like.%3B T3 gp1.6-like	gene	gene-HOU18_gp12	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp12
HOU18_gp13	LL2_013		HOU18_gp13	8375	8867	+	hypothetical protein	Close match to a hypothetical protein in phage T3. LL2-1 is an E.coli phage%2C and many of the general blast hits described uncharacterized proteins in many enterobacter phage.%3B T3 gp1.7-like	gene	gene-HOU18_gp13	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp13
HOU18_gp14	LL2_014		HOU18_gp14	8842	9000	+	hypothetical protein	Blast results suggest this protein is phage T3 gp1.8-like.%3B T3 gp1.7-like hypoethetical gene is directly upstream.%3B T3 gp1.8-like	gene	gene-HOU18_gp14	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp14
HOU18_gp15	LL2_015		HOU18_gp15	8975	9151	+	inhibitor of host RNAP	Close matches to T3 and T7 in the canonical database%2C both of which are for inhibitors of host bacterial RNA polymerase. Multiple blast results%2C many of which also predict RNA polymerase inhibitors.%3B T3/T7-like inhibitor of host bacterial RNA polymerase	gene	gene-HOU18_gp15	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp15
HOU18_gp16	LL2_016		HOU18_gp16	9192	9902	+	ssDNA binding protein	Similar to T3/T7 ssDNA-binding proteins. InterPro results indicate similarities to phage Helix-destablising proteins (which require ssDNA-binding) and suggest that the gene contains OB-fold domains (consistent with ssDNA-binding proteins).%3B Many blast hits are for enterobacter phages / podoviridae ssDNA-binding proteins.%3B T3/T7-like ssDNA binding protein	gene	gene-HOU18_gp16	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp16
HOU18_gp17	LL2_017		HOU18_gp17	9891	10379	+	HNH endonuclease	Four matches to the canonical phage database for hypothetical proteins. Many Blast hits for enterobacter phages/ podoviridae uncharacterized proteins.%3B The most conserved Blast hits are for HNH endonucleases.%3B InterPro result for an HNH domain within the gene.%3B contains HNH domain	gene	gene-HOU18_gp17	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp17
HOU18_gp18	LL2_018		HOU18_gp18	10330	10795	+	endonuclease	Strong sequence similarity to phage T7 and T3 endonucleases. Interpro results are all for endonucleases - strongest match suggests a T7 endonuclease. Many blast hits are for T7-like phage endonucleases.%3B T7 endonuclease I - like protein	gene	gene-HOU18_gp18	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp18
HOU18_gp19	LL2_019		HOU18_gp19	10780	11250	+	endolysin	Strong similarities to T3 and T7 lysozymes.%3B Interpro results indicate the presence of the N-acetylmuramoyl-L-alanine amidase domain.%3B The above points suggest this protein is a phage muralytic enzyme%2C which is consistent with the definition of a canonical endolysin.%3B T3/T7-like lysozyme	gene	gene-HOU18_gp19	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp19
HOU18_gp20	LL2_020		HOU18_gp20	11245	11362	+	hypothetical protein	Strong match to canonical phage T3 gp3.7.%3B T3 gp3.7-like	gene	gene-HOU18_gp20	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp20
HOU18_gp21	LL2_021		HOU18_gp21	11419	13130	+	DNA primase/helicase	Six results from the canonical phage database suggesting the gene is a helicase or DNA primase/helicase. Many blast hits suggest the same.%3B T3/T7-like DNA primase/helicase	gene	gene-HOU18_gp21	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp21
HOU18_gp22	LL2_022		HOU18_gp22	13214	13438	+	hypothetical protein	T7 superfamily gp4.3-like.%3B T7 gp4.3-like protein	gene	gene-HOU18_gp22	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp22
HOU18_gp23	LL2_023		HOU18_gp23	13438	13735	+	hypothetical protein	T7 superfamily gp4.5-like.%3B T7 gp4.5-like	gene	gene-HOU18_gp23	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp23
HOU18_gp24	LL2_024		HOU18_gp24	13792	15917	+	DNA polymerase	This phage (LL2-1) has consistently demonstrated similarities to the T3 and T7 phages. This gene has very strong matches to DNA polymerase for both T7 and T3%2C along with other canonical phages.%3B T3/T7-like DNA polymerase	gene	gene-HOU18_gp24	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp24
HOU18_gp25	LL2_025		HOU18_gp25	15917	16295	+	HNH endonuclease	A few matches of varying quality to the canonical phage database for predicted HNH endonuclease.%3B One very similar result from uniref BLAST for a T7-like virus endonuclease. Many other results are just for uncharacterized proteins.%3B InterPro result for an endonuclease.%3B InterPro result starts much later on in the sequence. The predicted gene has a possible SDS and ATG start in this area%2C so it is possible that the predicted gene is smaller than the actual gene call.%3B contains HNH domain	gene	gene-HOU18_gp25	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp25
HOU18_gp26	LL2_026		HOU18_gp26	16225	16536	+	host protein H-NS-interacting protein	Match to canonical phage database for a T3 H-NS-interacting protein.%3B InterPro Matches to T7 gene 5.5.%3B T7 gene 5.5 has been experimentally demonstrated to interactt with the host (E.coli) H-NS protein to inhibit gene silencing.%3B http://jb.asm.org/content/193/18/4881.full%3B T3 gp5.5-like H-NS-interacting protein	gene	gene-HOU18_gp26	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp26
HOU18_gp27	LL2_027		HOU18_gp27	16521	16742	+	hypothetical protein	T7 gp5.7-like.%3B T7 gp5.7-like	gene	gene-HOU18_gp27	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp27
HOU18_gp28	LL2_028		HOU18_gp28	16728	16921	+	host recBCD nuclease inhibitor	Homology to T7 %26 T3 phages for host recBCD nuclease inhibitor.%3B T7-like host recBCD nuclease inhibitor	gene	gene-HOU18_gp28	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp28
HOU18_gp29	LL2_029		HOU18_gp29	16906	17829	+	exonuclease	Similar to phage T3 and T7 exonucleases. Interpro results suggest the protein has a PIN-like domain - this is consistent with the prediction of an exonuclease.%3B T7/T3-like exonuclease. Contains PIN-like domain.	gene	gene-HOU18_gp29	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp29
HOU18_gp30	LL2_030		HOU18_gp30	17798	17924	+	hypothetical protein		gene	gene-HOU18_gp30	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp30
HOU18_gp31	LL2_031		HOU18_gp31	18004	18262	+	hypothetical protein	Close similarity to hypothetical proteins of T3 and T7. InterPro suggests the gene is similar to the uncharacterized gene 6.5 of T7-like Phages.%3B T7 gp6.5-like	gene	gene-HOU18_gp31	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp31
HOU18_gp32	LL2_032		HOU18_gp32	18255	18518	+	putative head protein	Matches hypothetical proteins of phage T3 and T7. InterPro suggests the gene is similar to gene 6.7 in T7-like phages.%3B Some experimental evidence seems to suggest that the T7-like gp 6.7 is a head protein%3B PMID: 16054667 provides evidence that gp6.7 of T7 is associated with the head%3B T7 gp6.7-like	gene	gene-HOU18_gp32	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp32
HOU18_gp33	LL2_033		HOU18_gp33	18535	18899	+	tail assembly protein	Matches to T3 and T7 assembly proteins. InterPro result for Bacteriophage T7-like virion assembly protein. Many blast hits are for virion assembly proteins.%3B T3/T7-like tail assembly protein	gene	gene-HOU18_gp33	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp33
HOU18_gp34	LL2_034		HOU18_gp34	18899	20517	+	portal protein	Strong homology to T3/T7 head-to-tail joining proteins.%3B Strong homolgy to phage SP6 head portal protein%3B Consistent blast hits for T3/T7-like phage head-to-tail joining proteins/ head-tail connector proteins/ portal proteins.%3B InterPro result for phage T3 gp8%2C which has been experimentally demonstrated to be a head-to-tail joining protein.%3B T3/T7-like portal protein	gene	gene-HOU18_gp34	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp34
HOU18_gp35	LL2_035		HOU18_gp35	20606	21551	+	capsid assembly protein	Matches T3 and T7 capsid assembly proteins. InterPro result also suggests T7-like capsid assembly protein. BLAST hits suggest the same.%3B Major/Minor capsid components are downstream.%3B T3/T7-like capsid assembly protein	gene	gene-HOU18_gp35	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp35
HOU18_gp36	LL2_036		HOU18_gp36	21696	22754	+	major capsid protein	T7-like major capsid protein	gene	gene-HOU18_gp36	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp36
HOU18_gp37	LL2_037		HOU18_gp37	22756	22888	+	minor capsid protein	Only two blast results. Highest similarity result suggests this is a minor capsid protein in a podoviridae phage.%3B putative minor capsid protein	gene	gene-HOU18_gp37	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp37
HOU18_gp38	LL2_038		HOU18_gp38	22954	23557	+	tail tubular protein	Matches tail tubular proteins for T3%2C T7 and one other canonical phage. Blast results also suggest some type of tail protein.%3B Some blast hits are for T7-like phage gp11. This gene product is one of the major components of the tail in canonical phage T7.%3B T7 gp11-like tail protein	gene	gene-HOU18_gp38	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp38
HOU18_gp39	LL2_039		HOU18_gp39	23563	25978	+	tail tubular protein	Matches to T3%2C T7 and many other BLAST results suggest this is the tail tubular protein B.%3B Gene directly upstream is predicted to express a tail tubular protein A.%3B T3/T7-like tail tubular protein B	gene	gene-HOU18_gp39	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp39
HOU18_gp40	LL2_040		HOU18_gp40	26038	26461	+	scaffold protein	Strong similarity to internal virion protein A of T3 and T7. InterPro results make the same predictions as matches to the canonical phage DB.%3B Internal virion A in T7 refers to GP13. InterPro and some blast results cite studies predicting this gene to be scaffold protein.%3B T3/T7-like internal virion protein	gene	gene-HOU18_gp40	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp40
HOU18_gp41	LL2_041		HOU18_gp41	26453	27057	+	internal virion protein	Strong similarity to internal virion protein B of T3 and T7. InterPro results make the same predictions as matches to the canonical phage DB.	gene	gene-HOU18_gp41	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp41
HOU18_gp42	LL2_042		HOU18_gp42	27050	29303	+	internal virion protein	Strong matches to internal virion protein C of T3 and T7.%3B T3/T7-like internal virion protein	gene	gene-HOU18_gp42	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp42
HOU18_gp43	LL2_043		HOU18_gp43	29312	33284	+	peptidoglycan hydrolase	Strong matches to internal virion protein D of T3 and T7. Directly adjacent to predicted virion protein C.%3B InterPro results suggest that this gene contains a Transglycosylase SLT domain%2C a lysozyme-like domain%2C and Prokaryotic transglycosylases signature.%3B Gene seems to be T7 gp16-like. T7 gp16 is known to have Peptidoglycan hydrolytic activity.%3B T3/T7-like internal virion protein D	gene	gene-HOU18_gp43	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp43
HOU18_gp44	LL2_044		HOU18_gp44	33346	35098	+	tail fiber protein	BLAST hits to cannonical phage DB for tail fiber proteins of phage T3 and T7. Very strong BLAST (general) hit to Kluyvera phage Kvp1 for a tail fiber.%3B T3/T7-like tail fiber protein	gene	gene-HOU18_gp44	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp44
HOU18_gp45	LL2_045		HOU18_gp45	35100	35635	+	tail fiber assembly protein	Strong matches to at least 5 canonical phages (mostly lambdoid) for tail fiber assembly proteins.%3B InterPro result for T4-like tail fiber assembly.%3B Interestingly%2C this gene seems similar to a tape measure chaperone proteins found in siphophages.%3B lambdoid gpG-like	gene	gene-HOU18_gp45	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp45
HOU18_gp46	LL2_046		HOU18_gp46	35682	35898	+	type II holin	Strong matches to T7 and T3 type II holin.%3B TMHMM detected a transmembrane protein in this gene. N out C in. This is expected in any phage holin protein.%3B T7/T3-like type II holin	gene	gene-HOU18_gp46	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp46
HOU18_gp47	LL2_047		HOU18_gp47	35891	36168	+	terminase small subunit	Strong similarty to DNA packaging proteins of T3 and T7. Interpro and Uniref exhibit similar results.%3B Multiple blast hits for various podoviridae phage terminases and DNA packaging proteins.%3B Upstream of putative large terminase subunit - this is the cannonical position of T7 TerS.%3B T3/T7-like DNA packaging protein	gene	gene-HOU18_gp47	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp47
HOU18_gp48	LL2_048		HOU18_gp48	36250	36711	+	i-spanin	Strong similarty to phage lambda Rz-like lysis protein in phages T3 and T7. Many BLAST hits in general are for lysis proteins.%3B The Rz-lysis protein typically consistutes the inner membrane spanin with an embedded outer membrane spanin.%3B A putative gene with a strong RBS ~9nt upstream of an ATG start codon is found embedded (predicted to be o-spanin).%3B Rz-like lysis protein	gene	gene-HOU18_gp48	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp48
HOU18_gp49	LL2_049		HOU18_gp49	36359	36628	+	o-spanin	Embedded into another gene that is predicted to be an Rz-like lysis gene (o-spanin) . This is consistent with lambda Rz/Rz1-like spanin compelxes.%3B Highly similar to NP_042009.1 (T7p56)%2C an rz1 like protein%3B Rz1-like lysis protein	gene	gene-HOU18_gp49	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp49
HOU18_gp50	LL2_050		HOU18_gp50	36674	38449	+	terminase large subunit	Strong homology to T3 DNA packaging protein B and T7 DNA maturation protein.%3B Strong homology to phage SP6p41 large terminase subunit.%3B Multiple blast hits for various podoviridae phage terminases and DNA packaging proteins.%3B T3/T7-like DNA packaging protein	gene	gene-HOU18_gp50	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp50
HOU18_gp51	LL2_051		HOU18_gp51	38682	38844	+	putative transmembrane protein	Matches hypothetical proteins in T3 and T7. TMHMM detected a N in C out transmembrane protein.%3B T7 gp19.5-like.%3B Contains a transmembrane domain. T7 gp19.5.	gene	gene-HOU18_gp51	http://www.ncbi.nlm.nih.gov/gene/?term=HOU18_gp51