##gff-version 3 #!gff-spec-version 1.21 #!processor NCBI annotwriter #!genome-build ASM357570v1 #!genome-build-accession NCBI_Assembly:GCF_003575705.1 ##sequence-region NC_048063.1 1 39382 ##species https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=2301650 NC_048063.1 RefSeq region 1 39382 . + . ID=NC_048063.1:1..39382;Dbxref=taxon:2301650;collected-by=Lauren Lessor;country=USA: College Station%2C TX;gbkey=Src;genome=genomic;isolation-source=wastewater treatment plant;mol_type=genomic DNA;nat-host=Escherichia coli;old-name=Escherichia coli podophage LL2 NC_048063.1 RefSeq gene 658 1129 . + . ID=gene-HOU18_gp01;Dbxref=GeneID:55003428;Name=HOU18_gp01;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp01;old_locus_tag=LL2_001 NC_048063.1 RefSeq CDS 671 1129 . + 0 ID=cds-YP_009812313.1;Parent=gene-HOU18_gp01;Dbxref=Genbank:YP_009812313.1,GeneID:55003428;Name=YP_009812313.1;Note=Strong matches to canonical phage DB%2C InterPro and uniref for a gene encoding S-adenosyl-L-methionine hydrolase.%3B T3-like S-adenosyl-L-methionine hydrolase;gbkey=CDS;locus_tag=HOU18_gp01;product=S-adenosyl-L-methionine hydrolase;protein_id=YP_009812313.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 658 662 . + . ID=id-HOU18_gp01;Dbxref=GeneID:55003428;Note=LL2_001;gbkey=regulatory;locus_tag=HOU18_gp01;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 1142 1402 . + . ID=gene-HOU18_gp02;Dbxref=GeneID:55003378;Name=HOU18_gp02;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp02;old_locus_tag=LL2_002 NC_048063.1 RefSeq CDS 1154 1402 . + 0 ID=cds-YP_009812314.1;Parent=gene-HOU18_gp02;Dbxref=Genbank:YP_009812314.1,GeneID:55003378;Name=YP_009812314.1;Note=Some partial matches to uncharacterized phage proteins. May be conserved%2C but not highly so.;gbkey=CDS;locus_tag=HOU18_gp02;product=hypothetical protein;protein_id=YP_009812314.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 1142 1145 . + . ID=id-HOU18_gp02;Dbxref=GeneID:55003378;Note=LL2_002;gbkey=regulatory;locus_tag=HOU18_gp02;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 1388 1605 . + . ID=gene-HOU18_gp03;Dbxref=GeneID:55003379;Name=HOU18_gp03;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp03;old_locus_tag=LL2_003 NC_048063.1 RefSeq CDS 1402 1605 . + 0 ID=cds-YP_009812315.1;Parent=gene-HOU18_gp03;Dbxref=Genbank:YP_009812315.1,GeneID:55003379;Name=YP_009812315.1;Note=TMHMM detected a N out C in transmembrane domain.%3B InterPro recognizes a non-cytoplasmid domain%2C a transmembrane domain and a cytoplasmic domain.%3B T3 gp0.6-like.%3B Contains a transmembrane domain.T3 gp0.6-like protein.;gbkey=CDS;locus_tag=HOU18_gp03;product=putative transmembrane protein;protein_id=YP_009812315.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 1388 1391 . + . ID=id-HOU18_gp03;Dbxref=GeneID:55003379;Note=LL2_003;gbkey=regulatory;locus_tag=HOU18_gp03;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 1575 1762 . + . ID=gene-HOU18_gp04;Dbxref=GeneID:55003380;Name=HOU18_gp04;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp04;old_locus_tag=LL2_004 NC_048063.1 RefSeq CDS 1592 1762 . + 0 ID=cds-YP_009812316.1;Parent=gene-HOU18_gp04;Dbxref=Genbank:YP_009812316.1,GeneID:55003380;Name=YP_009812316.1;Note=Matches uncharacterized protein produced by T3 gene 0.65.%3B Match to T3 gene 0.6 is directly upstream.%3B T3 gp0.65-like protein;gbkey=CDS;locus_tag=HOU18_gp04;product=hypothetical protein;protein_id=YP_009812316.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 1579 1582 . + . ID=id-HOU18_gp04;Dbxref=GeneID:55003380;Note=LL2_004;gbkey=regulatory;locus_tag=HOU18_gp04;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 1764 2889 . + . ID=gene-HOU18_gp05;Dbxref=GeneID:55003381;Name=HOU18_gp05;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp05;old_locus_tag=LL2_005 NC_048063.1 RefSeq CDS 1777 2889 . + 0 ID=cds-YP_009812317.1;Parent=gene-HOU18_gp05;Dbxref=Genbank:YP_009812317.1,GeneID:55003381;Name=YP_009812317.1;Note=Strong matches to T3 and T7 protein kinases.%3B InterPro and various BLAST results suggest a T7-like protein kinase.%3B T3/T7-like protein kinase;gbkey=CDS;locus_tag=HOU18_gp05;product=protein kinase;protein_id=YP_009812317.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 1764 1767 . + . ID=id-HOU18_gp05;Dbxref=GeneID:55003381;Note=LL2_005;gbkey=regulatory;locus_tag=HOU18_gp05;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 2950 5614 . + . ID=gene-HOU18_gp06;Dbxref=GeneID:55003382;Name=HOU18_gp06;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp06;old_locus_tag=LL2_006 NC_048063.1 RefSeq CDS 2960 5614 . + 0 ID=cds-YP_009812318.1;Parent=gene-HOU18_gp06;Dbxref=Genbank:YP_009812318.1,GeneID:55003382;Name=YP_009812318.1;Note=Matches RNA polymerase of phages T3 and T7%2C and many other T7-like phages.%3B T3/T7-like RNA polymerase;gbkey=CDS;locus_tag=HOU18_gp06;product=RNA polymerase;protein_id=YP_009812318.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 2950 2954 . + . ID=id-HOU18_gp06;Dbxref=GeneID:55003382;Note=LL2_006;gbkey=regulatory;locus_tag=HOU18_gp06;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 5687 5973 . + . ID=gene-HOU18_gp07;Dbxref=GeneID:55003383;Name=HOU18_gp07;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp07;old_locus_tag=LL2_007 NC_048063.1 RefSeq CDS 5701 5973 . + 0 ID=cds-YP_009812319.1;Parent=gene-HOU18_gp07;Dbxref=Genbank:YP_009812319.1,GeneID:55003383;Name=YP_009812319.1;Note=Matches uncharacterized gp1.05 of phage T3.%3B T3 gp1.05-like;gbkey=CDS;locus_tag=HOU18_gp07;product=hypothetical protein;protein_id=YP_009812319.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 5689 5691 . + . ID=id-HOU18_gp07;Dbxref=GeneID:55003383;Note=LL2_007;gbkey=regulatory;locus_tag=HOU18_gp07;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 6053 6206 . + . ID=gene-HOU18_gp08;Dbxref=GeneID:55003384;Name=HOU18_gp08;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp08;old_locus_tag=LL2_008 NC_048063.1 RefSeq CDS 6066 6206 . + 0 ID=cds-YP_009812320.1;Parent=gene-HOU18_gp08;Dbxref=Genbank:YP_009812320.1,GeneID:55003384;Name=YP_009812320.1;Note=T7 gp1.1-like%3B T7 gp1.1-like;gbkey=CDS;locus_tag=HOU18_gp08;product=hypothetical protein;protein_id=YP_009812320.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 6053 6057 . + . ID=id-HOU18_gp08;Dbxref=GeneID:55003384;Note=LL2_008;gbkey=regulatory;locus_tag=HOU18_gp08;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 6194 6481 . + . ID=gene-HOU18_gp09;Dbxref=GeneID:55003385;Name=HOU18_gp09;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp09;old_locus_tag=LL2_009 NC_048063.1 RefSeq CDS 6209 6481 . + 0 ID=cds-YP_009812321.1;Parent=gene-HOU18_gp09;Dbxref=Genbank:YP_009812321.1,GeneID:55003385;Name=YP_009812321.1;Note=Strong matches to phage T3/T7 host dGTPase inhibitor. Interpro results are for proteins in the same family as dGTPase inhibitors.%3B T3/T7-like host dGTPase inhibitor;gbkey=CDS;locus_tag=HOU18_gp09;product=host dGTPase inhibitor;protein_id=YP_009812321.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 6194 6197 . + . ID=id-NC_048063.1:6194..6197;Note=LL2_009;gbkey=regulatory;locus_tag=LL2_009;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 6563 7622 . + . ID=gene-HOU18_gp10;Dbxref=GeneID:55003386;Name=HOU18_gp10;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp10;old_locus_tag=LL2_010 NC_048063.1 RefSeq CDS 6576 7622 . + 0 ID=cds-YP_009812322.1;Parent=gene-HOU18_gp10;Dbxref=Genbank:YP_009812322.1,GeneID:55003386;Name=YP_009812322.1;gbkey=CDS;locus_tag=HOU18_gp10;product=ATP-dependent DNA ligase;protein_id=YP_009812322.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 6563 6566 . + . ID=id-HOU18_gp10;Dbxref=GeneID:55003386;Note=LL2_010;gbkey=regulatory;locus_tag=HOU18_gp10;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 7538 8025 . + . ID=gene-HOU18_gp11;Dbxref=GeneID:55003387;Name=HOU18_gp11;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp11;old_locus_tag=LL2_011 NC_048063.1 RefSeq CDS 7552 8025 . + 0 ID=cds-YP_009812323.1;Parent=gene-HOU18_gp11;Dbxref=Genbank:YP_009812323.1,GeneID:55003387;Name=YP_009812323.1;Note=14 matches to the canonical phage database for HNH endonucleases. Interpro results are linked to descriptions of HNH endonucleases/endonucleases.%3B Multiple strong blast hits and InterPro results for endonucleases.%3B JG-RBS missing/deleted?%3B conserved HNH endounclease;gbkey=CDS;locus_tag=HOU18_gp11;product=HNH endonuclease;protein_id=YP_009812323.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 7538 7542 . + . ID=id-HOU18_gp11;Dbxref=GeneID:55003387;Note=LL2_011;gbkey=regulatory;locus_tag=HOU18_gp11;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 8118 8388 . + . ID=gene-HOU18_gp12;Dbxref=GeneID:55003388;Name=HOU18_gp12;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp12;old_locus_tag=LL2_012 NC_048063.1 RefSeq CDS 8131 8388 . + 0 ID=cds-YP_009812324.1;Parent=gene-HOU18_gp12;Dbxref=Genbank:YP_009812324.1,GeneID:55003388;Name=YP_009812324.1;Note=Matches Hypothetical genes of phages T7 and T3. Many blast hits to uncharacterized or hypothetical proteins of various podophages.%3B Blast results suggest this protein is T3 gp1.6-like.%3B T3 gp1.6-like;gbkey=CDS;locus_tag=HOU18_gp12;product=hypothetical protein;protein_id=YP_009812324.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 8118 8122 . + . ID=id-HOU18_gp12;Dbxref=GeneID:55003388;Note=LL2_012;gbkey=regulatory;locus_tag=HOU18_gp12;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 8375 8867 . + . ID=gene-HOU18_gp13;Dbxref=GeneID:55003389;Name=HOU18_gp13;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp13;old_locus_tag=LL2_013 NC_048063.1 RefSeq CDS 8388 8867 . + 0 ID=cds-YP_009812325.1;Parent=gene-HOU18_gp13;Dbxref=Genbank:YP_009812325.1,GeneID:55003389;Name=YP_009812325.1;Note=Close match to a hypothetical protein in phage T3. LL2-1 is an E.coli phage%2C and many of the general blast hits described uncharacterized proteins in many enterobacter phage.%3B T3 gp1.7-like;gbkey=CDS;locus_tag=HOU18_gp13;product=hypothetical protein;protein_id=YP_009812325.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 8375 8381 . + . ID=id-NC_048063.1:8375..8381;Note=LL2_013;gbkey=regulatory;locus_tag=LL2_013;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 8842 9000 . + . ID=gene-HOU18_gp14;Dbxref=GeneID:55003390;Name=HOU18_gp14;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp14;old_locus_tag=LL2_014 NC_048063.1 RefSeq CDS 8854 9000 . + 0 ID=cds-YP_009812326.1;Parent=gene-HOU18_gp14;Dbxref=Genbank:YP_009812326.1,GeneID:55003390;Name=YP_009812326.1;Note=Blast results suggest this protein is phage T3 gp1.8-like.%3B T3 gp1.7-like hypoethetical gene is directly upstream.%3B T3 gp1.8-like;gbkey=CDS;locus_tag=HOU18_gp14;product=hypothetical protein;protein_id=YP_009812326.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 8842 8846 . + . ID=id-HOU18_gp14;Dbxref=GeneID:55003390;Note=LL2_014;gbkey=regulatory;locus_tag=HOU18_gp14;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 8975 9151 . + . ID=gene-HOU18_gp15;Dbxref=GeneID:55003391;Name=HOU18_gp15;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp15;old_locus_tag=LL2_015 NC_048063.1 RefSeq CDS 8987 9151 . + 0 ID=cds-YP_009812327.1;Parent=gene-HOU18_gp15;Dbxref=Genbank:YP_009812327.1,GeneID:55003391;Name=YP_009812327.1;Note=Close matches to T3 and T7 in the canonical database%2C both of which are for inhibitors of host bacterial RNA polymerase. Multiple blast results%2C many of which also predict RNA polymerase inhibitors.%3B T3/T7-like inhibitor of host bacterial RNA polymerase;gbkey=CDS;locus_tag=HOU18_gp15;product=inhibitor of host RNAP;protein_id=YP_009812327.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 8975 8979 . + . ID=id-NC_048063.1:8975..8979;Note=LL2_015;gbkey=regulatory;locus_tag=LL2_015;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 9192 9902 . + . ID=gene-HOU18_gp16;Dbxref=GeneID:55003392;Name=HOU18_gp16;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp16;old_locus_tag=LL2_016 NC_048063.1 RefSeq CDS 9204 9902 . + 0 ID=cds-YP_009812328.1;Parent=gene-HOU18_gp16;Dbxref=Genbank:YP_009812328.1,GeneID:55003392;Name=YP_009812328.1;Note=Similar to T3/T7 ssDNA-binding proteins. InterPro results indicate similarities to phage Helix-destablising proteins (which require ssDNA-binding) and suggest that the gene contains OB-fold domains (consistent with ssDNA-binding proteins).%3B Many blast hits are for enterobacter phages / podoviridae ssDNA-binding proteins.%3B T3/T7-like ssDNA binding protein;gbkey=CDS;locus_tag=HOU18_gp16;product=ssDNA binding protein;protein_id=YP_009812328.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 9192 9196 . + . ID=id-HOU18_gp16;Dbxref=GeneID:55003392;Note=LL2_016;gbkey=regulatory;locus_tag=HOU18_gp16;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 9891 10379 . + . ID=gene-HOU18_gp17;Dbxref=GeneID:55003393;Name=HOU18_gp17;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp17;old_locus_tag=LL2_017 NC_048063.1 RefSeq CDS 9903 10379 . + 0 ID=cds-YP_009812329.1;Parent=gene-HOU18_gp17;Dbxref=Genbank:YP_009812329.1,GeneID:55003393;Name=YP_009812329.1;Note=Four matches to the canonical phage database for hypothetical proteins. Many Blast hits for enterobacter phages/ podoviridae uncharacterized proteins.%3B The most conserved Blast hits are for HNH endonucleases.%3B InterPro result for an HNH domain within the gene.%3B contains HNH domain;gbkey=CDS;locus_tag=HOU18_gp17;product=HNH endonuclease;protein_id=YP_009812329.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 9891 9894 . + . ID=id-HOU18_gp17;Dbxref=GeneID:55003393;Note=LL2_017;gbkey=regulatory;locus_tag=HOU18_gp17;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 10330 10795 . + . ID=gene-HOU18_gp18;Dbxref=GeneID:55003394;Name=HOU18_gp18;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp18;old_locus_tag=LL2_018 NC_048063.1 RefSeq CDS 10340 10795 . + 0 ID=cds-YP_009812330.1;Parent=gene-HOU18_gp18;Dbxref=Genbank:YP_009812330.1,GeneID:55003394;Name=YP_009812330.1;Note=Strong sequence similarity to phage T7 and T3 endonucleases. Interpro results are all for endonucleases - strongest match suggests a T7 endonuclease. Many blast hits are for T7-like phage endonucleases.%3B T7 endonuclease I - like protein;gbkey=CDS;locus_tag=HOU18_gp18;product=endonuclease;protein_id=YP_009812330.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 10330 10334 . + . ID=id-HOU18_gp18;Dbxref=GeneID:55003394;Note=LL2_018;gbkey=regulatory;locus_tag=HOU18_gp18;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 10780 11250 . + . ID=gene-HOU18_gp19;Dbxref=GeneID:55003395;Name=HOU18_gp19;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp19;old_locus_tag=LL2_019 NC_048063.1 RefSeq CDS 10795 11250 . + 0 ID=cds-YP_009812331.1;Parent=gene-HOU18_gp19;Dbxref=Genbank:YP_009812331.1,GeneID:55003395;Name=YP_009812331.1;Note=Strong similarities to T3 and T7 lysozymes.%3B Interpro results indicate the presence of the N-acetylmuramoyl-L-alanine amidase domain.%3B The above points suggest this protein is a phage muralytic enzyme%2C which is consistent with the definition of a canonical endolysin.%3B T3/T7-like lysozyme;gbkey=CDS;locus_tag=HOU18_gp19;product=endolysin;protein_id=YP_009812331.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 10780 10784 . + . ID=id-NC_048063.1:10780..10784;Note=LL2_019;gbkey=regulatory;locus_tag=LL2_019;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 11245 11362 . + . ID=gene-HOU18_gp20;Dbxref=GeneID:55003396;Name=HOU18_gp20;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp20;old_locus_tag=LL2_020 NC_048063.1 RefSeq CDS 11255 11362 . + 0 ID=cds-YP_009812332.1;Parent=gene-HOU18_gp20;Dbxref=Genbank:YP_009812332.1,GeneID:55003396;Name=YP_009812332.1;Note=Strong match to canonical phage T3 gp3.7.%3B T3 gp3.7-like;gbkey=CDS;locus_tag=HOU18_gp20;product=hypothetical protein;protein_id=YP_009812332.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 11245 11248 . + . ID=id-HOU18_gp20;Dbxref=GeneID:55003396;Note=LL2_020;gbkey=regulatory;locus_tag=HOU18_gp20;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 11419 13130 . + . ID=gene-HOU18_gp21;Dbxref=GeneID:55003397;Name=HOU18_gp21;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp21;old_locus_tag=LL2_021 NC_048063.1 RefSeq CDS 11430 13130 . + 0 ID=cds-YP_009812333.1;Parent=gene-HOU18_gp21;Dbxref=Genbank:YP_009812333.1,GeneID:55003397;Name=YP_009812333.1;Note=Six results from the canonical phage database suggesting the gene is a helicase or DNA primase/helicase. Many blast hits suggest the same.%3B T3/T7-like DNA primase/helicase;gbkey=CDS;locus_tag=HOU18_gp21;product=DNA primase/helicase;protein_id=YP_009812333.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 11419 11422 . + . ID=id-HOU18_gp21;Dbxref=GeneID:55003397;Note=LL2_021;gbkey=regulatory;locus_tag=HOU18_gp21;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 13214 13438 . + . ID=gene-HOU18_gp22;Dbxref=GeneID:55003398;Name=HOU18_gp22;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp22;old_locus_tag=LL2_022 NC_048063.1 RefSeq CDS 13226 13438 . + 0 ID=cds-YP_009812334.1;Parent=gene-HOU18_gp22;Dbxref=Genbank:YP_009812334.1,GeneID:55003398;Name=YP_009812334.1;Note=T7 superfamily gp4.3-like.%3B T7 gp4.3-like protein;gbkey=CDS;locus_tag=HOU18_gp22;product=hypothetical protein;protein_id=YP_009812334.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 13214 13218 . + . ID=id-HOU18_gp22;Dbxref=GeneID:55003398;Note=LL2_022;gbkey=regulatory;locus_tag=HOU18_gp22;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 13438 13735 . + . ID=gene-HOU18_gp23;Dbxref=GeneID:55003399;Name=HOU18_gp23;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp23;old_locus_tag=LL2_023 NC_048063.1 RefSeq CDS 13451 13735 . + 0 ID=cds-YP_009812335.1;Parent=gene-HOU18_gp23;Dbxref=Genbank:YP_009812335.1,GeneID:55003399;Name=YP_009812335.1;Note=T7 superfamily gp4.5-like.%3B T7 gp4.5-like;gbkey=CDS;locus_tag=HOU18_gp23;product=hypothetical protein;protein_id=YP_009812335.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 13438 13442 . + . ID=id-HOU18_gp23;Dbxref=GeneID:55003399;Note=LL2_023;gbkey=regulatory;locus_tag=HOU18_gp23;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 13792 15917 . + . ID=gene-HOU18_gp24;Dbxref=GeneID:55003400;Name=HOU18_gp24;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp24;old_locus_tag=LL2_024 NC_048063.1 RefSeq CDS 13803 15917 . + 0 ID=cds-YP_009812336.1;Parent=gene-HOU18_gp24;Dbxref=Genbank:YP_009812336.1,GeneID:55003400;Name=YP_009812336.1;Note=This phage (LL2-1) has consistently demonstrated similarities to the T3 and T7 phages. This gene has very strong matches to DNA polymerase for both T7 and T3%2C along with other canonical phages.%3B T3/T7-like DNA polymerase;gbkey=CDS;locus_tag=HOU18_gp24;product=DNA polymerase;protein_id=YP_009812336.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 13792 13797 . + . ID=id-HOU18_gp24;Dbxref=GeneID:55003400;Note=LL2_024;gbkey=regulatory;locus_tag=HOU18_gp24;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 15917 16295 . + . ID=gene-HOU18_gp25;Dbxref=GeneID:55003401;Name=HOU18_gp25;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp25;old_locus_tag=LL2_025 NC_048063.1 RefSeq CDS 15927 16295 . + 0 ID=cds-YP_009812337.1;Parent=gene-HOU18_gp25;Dbxref=Genbank:YP_009812337.1,GeneID:55003401;Name=YP_009812337.1;Note=A few matches of varying quality to the canonical phage database for predicted HNH endonuclease.%3B One very similar result from uniref BLAST for a T7-like virus endonuclease. Many other results are just for uncharacterized proteins.%3B InterPro result for an endonuclease.%3B InterPro result starts much later on in the sequence. The predicted gene has a possible SDS and ATG start in this area%2C so it is possible that the predicted gene is smaller than the actual gene call.%3B contains HNH domain;gbkey=CDS;locus_tag=HOU18_gp25;product=HNH endonuclease;protein_id=YP_009812337.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 15917 15921 . + . ID=id-HOU18_gp25;Dbxref=GeneID:55003401;Note=LL2_025;gbkey=regulatory;locus_tag=HOU18_gp25;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 16225 16536 . + . ID=gene-HOU18_gp26;Dbxref=GeneID:55003402;Name=HOU18_gp26;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp26;old_locus_tag=LL2_026 NC_048063.1 RefSeq CDS 16237 16536 . + 0 ID=cds-YP_009812338.1;Parent=gene-HOU18_gp26;Dbxref=Genbank:YP_009812338.1,GeneID:55003402;Name=YP_009812338.1;Note=Match to canonical phage database for a T3 H-NS-interacting protein.%3B InterPro Matches to T7 gene 5.5.%3B T7 gene 5.5 has been experimentally demonstrated to interactt with the host (E.coli) H-NS protein to inhibit gene silencing.%3B http://jb.asm.org/content/193/18/4881.full%3B T3 gp5.5-like H-NS-interacting protein;gbkey=CDS;locus_tag=HOU18_gp26;product=host protein H-NS-interacting protein;protein_id=YP_009812338.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 16225 16229 . + . ID=id-HOU18_gp26;Dbxref=GeneID:55003402;Note=LL2_026;gbkey=regulatory;locus_tag=HOU18_gp26;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 16521 16742 . + . ID=gene-HOU18_gp27;Dbxref=GeneID:55003403;Name=HOU18_gp27;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp27;old_locus_tag=LL2_027 NC_048063.1 RefSeq CDS 16533 16742 . + 0 ID=cds-YP_009812339.1;Parent=gene-HOU18_gp27;Dbxref=Genbank:YP_009812339.1,GeneID:55003403;Name=YP_009812339.1;Note=T7 gp5.7-like.%3B T7 gp5.7-like;gbkey=CDS;locus_tag=HOU18_gp27;product=hypothetical protein;protein_id=YP_009812339.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 16521 16526 . + . ID=id-HOU18_gp27;Dbxref=GeneID:55003403;Note=LL2_027;gbkey=regulatory;locus_tag=HOU18_gp27;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 16728 16921 . + . ID=gene-HOU18_gp28;Dbxref=GeneID:55003404;Name=HOU18_gp28;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp28;old_locus_tag=LL2_028 NC_048063.1 RefSeq CDS 16739 16921 . + 0 ID=cds-YP_009812340.1;Parent=gene-HOU18_gp28;Dbxref=Genbank:YP_009812340.1,GeneID:55003404;Name=YP_009812340.1;Note=Homology to T7 %26 T3 phages for host recBCD nuclease inhibitor.%3B T7-like host recBCD nuclease inhibitor;gbkey=CDS;locus_tag=HOU18_gp28;product=host recBCD nuclease inhibitor;protein_id=YP_009812340.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 16728 16733 . + . ID=id-HOU18_gp28;Dbxref=GeneID:55003404;Note=LL2_028;gbkey=regulatory;locus_tag=HOU18_gp28;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 16906 17829 . + . ID=gene-HOU18_gp29;Dbxref=GeneID:55003405;Name=HOU18_gp29;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp29;old_locus_tag=LL2_029 NC_048063.1 RefSeq CDS 16918 17829 . + 0 ID=cds-YP_009812341.1;Parent=gene-HOU18_gp29;Dbxref=Genbank:YP_009812341.1,GeneID:55003405;Name=YP_009812341.1;Note=Similar to phage T3 and T7 exonucleases. Interpro results suggest the protein has a PIN-like domain - this is consistent with the prediction of an exonuclease.%3B T7/T3-like exonuclease. Contains PIN-like domain.;gbkey=CDS;locus_tag=HOU18_gp29;product=exonuclease;protein_id=YP_009812341.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 16906 16910 . + . ID=id-NC_048063.1:16906..16910;Note=LL2_029;gbkey=regulatory;locus_tag=LL2_029;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 17798 17924 . + . ID=gene-HOU18_gp30;Dbxref=GeneID:55003406;Name=HOU18_gp30;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp30;old_locus_tag=LL2_030 NC_048063.1 RefSeq CDS 17811 17924 . + 0 ID=cds-YP_009812342.1;Parent=gene-HOU18_gp30;Dbxref=Genbank:YP_009812342.1,GeneID:55003406;Name=YP_009812342.1;gbkey=CDS;locus_tag=HOU18_gp30;product=hypothetical protein;protein_id=YP_009812342.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 17798 17802 . + . ID=id-HOU18_gp30;Dbxref=GeneID:55003406;Note=LL2_030;gbkey=regulatory;locus_tag=HOU18_gp30;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 18004 18262 . + . ID=gene-HOU18_gp31;Dbxref=GeneID:55003407;Name=HOU18_gp31;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp31;old_locus_tag=LL2_031 NC_048063.1 RefSeq CDS 18017 18262 . + 0 ID=cds-YP_009812343.1;Parent=gene-HOU18_gp31;Dbxref=Genbank:YP_009812343.1,GeneID:55003407;Name=YP_009812343.1;Note=Close similarity to hypothetical proteins of T3 and T7. InterPro suggests the gene is similar to the uncharacterized gene 6.5 of T7-like Phages.%3B T7 gp6.5-like;gbkey=CDS;locus_tag=HOU18_gp31;product=hypothetical protein;protein_id=YP_009812343.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 18006 18010 . + . ID=id-HOU18_gp31;Dbxref=GeneID:55003407;Note=LL2_031;gbkey=regulatory;locus_tag=HOU18_gp31;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 18255 18518 . + . ID=gene-HOU18_gp32;Dbxref=GeneID:55003408;Name=HOU18_gp32;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp32;old_locus_tag=LL2_032 NC_048063.1 RefSeq CDS 18267 18518 . + 0 ID=cds-YP_009812344.1;Parent=gene-HOU18_gp32;Dbxref=Genbank:YP_009812344.1,GeneID:55003408;Name=YP_009812344.1;Note=Matches hypothetical proteins of phage T3 and T7. InterPro suggests the gene is similar to gene 6.7 in T7-like phages.%3B Some experimental evidence seems to suggest that the T7-like gp 6.7 is a head protein%3B PMID: 16054667 provides evidence that gp6.7 of T7 is associated with the head%3B T7 gp6.7-like;gbkey=CDS;locus_tag=HOU18_gp32;product=putative head protein;protein_id=YP_009812344.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 18255 18259 . + . ID=id-NC_048063.1:18255..18259;Note=LL2_032;gbkey=regulatory;locus_tag=LL2_032;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 18535 18899 . + . ID=gene-HOU18_gp33;Dbxref=GeneID:55003409;Name=HOU18_gp33;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp33;old_locus_tag=LL2_033 NC_048063.1 RefSeq CDS 18546 18899 . + 0 ID=cds-YP_009812345.1;Parent=gene-HOU18_gp33;Dbxref=Genbank:YP_009812345.1,GeneID:55003409;Name=YP_009812345.1;Note=Matches to T3 and T7 assembly proteins. InterPro result for Bacteriophage T7-like virion assembly protein. Many blast hits are for virion assembly proteins.%3B T3/T7-like tail assembly protein;gbkey=CDS;locus_tag=HOU18_gp33;product=tail assembly protein;protein_id=YP_009812345.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 18535 18538 . + . ID=id-HOU18_gp33;Dbxref=GeneID:55003409;Note=LL2_033;gbkey=regulatory;locus_tag=HOU18_gp33;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 18899 20517 . + . ID=gene-HOU18_gp34;Dbxref=GeneID:55003410;Name=HOU18_gp34;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp34;old_locus_tag=LL2_034 NC_048063.1 RefSeq CDS 18910 20517 . + 0 ID=cds-YP_009812346.1;Parent=gene-HOU18_gp34;Dbxref=Genbank:YP_009812346.1,GeneID:55003410;Name=YP_009812346.1;Note=Strong homology to T3/T7 head-to-tail joining proteins.%3B Strong homolgy to phage SP6 head portal protein%3B Consistent blast hits for T3/T7-like phage head-to-tail joining proteins/ head-tail connector proteins/ portal proteins.%3B InterPro result for phage T3 gp8%2C which has been experimentally demonstrated to be a head-to-tail joining protein.%3B T3/T7-like portal protein;gbkey=CDS;locus_tag=HOU18_gp34;product=portal protein;protein_id=YP_009812346.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 18899 18904 . + . ID=id-HOU18_gp34;Dbxref=GeneID:55003410;Note=LL2_034;gbkey=regulatory;locus_tag=HOU18_gp34;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 20606 21551 . + . ID=gene-HOU18_gp35;Dbxref=GeneID:55003411;Name=HOU18_gp35;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp35;old_locus_tag=LL2_035 NC_048063.1 RefSeq CDS 20619 21551 . + 0 ID=cds-YP_009812347.1;Parent=gene-HOU18_gp35;Dbxref=Genbank:YP_009812347.1,GeneID:55003411;Name=YP_009812347.1;Note=Matches T3 and T7 capsid assembly proteins. InterPro result also suggests T7-like capsid assembly protein. BLAST hits suggest the same.%3B Major/Minor capsid components are downstream.%3B T3/T7-like capsid assembly protein;gbkey=CDS;locus_tag=HOU18_gp35;product=capsid assembly protein;protein_id=YP_009812347.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 20606 20610 . + . ID=id-HOU18_gp35;Dbxref=GeneID:55003411;Note=LL2_035;gbkey=regulatory;locus_tag=HOU18_gp35;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 21696 22754 . + . ID=gene-HOU18_gp36;Dbxref=GeneID:55003412;Name=HOU18_gp36;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp36;old_locus_tag=LL2_036 NC_048063.1 RefSeq CDS 21708 22754 . + 0 ID=cds-YP_009812348.1;Parent=gene-HOU18_gp36;Dbxref=Genbank:YP_009812348.1,GeneID:55003412;Name=YP_009812348.1;Note=T7-like major capsid protein;gbkey=CDS;locus_tag=HOU18_gp36;product=major capsid protein;protein_id=YP_009812348.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 21696 21700 . + . ID=id-HOU18_gp36;Dbxref=GeneID:55003412;Note=LL2_036;gbkey=regulatory;locus_tag=HOU18_gp36;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 22756 22888 . + . ID=gene-HOU18_gp37;Dbxref=GeneID:55003413;Name=HOU18_gp37;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp37;old_locus_tag=LL2_037 NC_048063.1 RefSeq CDS 22772 22888 . + 0 ID=cds-YP_009812349.1;Parent=gene-HOU18_gp37;Dbxref=Genbank:YP_009812349.1,GeneID:55003413;Name=YP_009812349.1;Note=Only two blast results. Highest similarity result suggests this is a minor capsid protein in a podoviridae phage.%3B putative minor capsid protein;gbkey=CDS;locus_tag=HOU18_gp37;product=minor capsid protein;protein_id=YP_009812349.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 22756 22758 . + . ID=id-HOU18_gp37;Dbxref=GeneID:55003413;Note=LL2_037;gbkey=regulatory;locus_tag=HOU18_gp37;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq terminator 22897 22935 . + . ID=id-NC_048063.1:22897..22935;gbkey=regulatory;regulatory_class=terminator NC_048063.1 RefSeq gene 22954 23557 . + . ID=gene-HOU18_gp38;Dbxref=GeneID:55003414;Name=HOU18_gp38;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp38;old_locus_tag=LL2_038 NC_048063.1 RefSeq CDS 22967 23557 . + 0 ID=cds-YP_009812350.1;Parent=gene-HOU18_gp38;Dbxref=Genbank:YP_009812350.1,GeneID:55003414;Name=YP_009812350.1;Note=Matches tail tubular proteins for T3%2C T7 and one other canonical phage. Blast results also suggest some type of tail protein.%3B Some blast hits are for T7-like phage gp11. This gene product is one of the major components of the tail in canonical phage T7.%3B T7 gp11-like tail protein;gbkey=CDS;locus_tag=HOU18_gp38;product=tail tubular protein;protein_id=YP_009812350.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 22954 22960 . + . ID=id-HOU18_gp38;Dbxref=GeneID:55003414;Note=LL2_038;gbkey=regulatory;locus_tag=HOU18_gp38;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 23563 25978 . + . ID=gene-HOU18_gp39;Dbxref=GeneID:55003415;Name=HOU18_gp39;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp39;old_locus_tag=LL2_039 NC_048063.1 RefSeq CDS 23573 25978 . + 0 ID=cds-YP_009812351.1;Parent=gene-HOU18_gp39;Dbxref=Genbank:YP_009812351.1,GeneID:55003415;Name=YP_009812351.1;Note=Matches to T3%2C T7 and many other BLAST results suggest this is the tail tubular protein B.%3B Gene directly upstream is predicted to express a tail tubular protein A.%3B T3/T7-like tail tubular protein B;gbkey=CDS;locus_tag=HOU18_gp39;product=tail tubular protein;protein_id=YP_009812351.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 23563 23567 . + . ID=id-HOU18_gp39;Dbxref=GeneID:55003415;Note=LL2_039;gbkey=regulatory;locus_tag=HOU18_gp39;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 26038 26461 . + . ID=gene-HOU18_gp40;Dbxref=GeneID:55003416;Name=HOU18_gp40;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp40;old_locus_tag=LL2_040 NC_048063.1 RefSeq CDS 26051 26461 . + 0 ID=cds-YP_009812352.1;Parent=gene-HOU18_gp40;Dbxref=Genbank:YP_009812352.1,GeneID:55003416;Name=YP_009812352.1;Note=Strong similarity to internal virion protein A of T3 and T7. InterPro results make the same predictions as matches to the canonical phage DB.%3B Internal virion A in T7 refers to GP13. InterPro and some blast results cite studies predicting this gene to be scaffold protein.%3B T3/T7-like internal virion protein;gbkey=CDS;locus_tag=HOU18_gp40;product=scaffold protein;protein_id=YP_009812352.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 26038 26041 . + . ID=id-HOU18_gp40;Dbxref=GeneID:55003416;Note=LL2_040;gbkey=regulatory;locus_tag=HOU18_gp40;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq ribosome_entry_site 26453 26458 . + . ID=id-HOU18_gp40-2;Dbxref=GeneID:55003416;Note=LL2_041;gbkey=regulatory;locus_tag=HOU18_gp40;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 26453 27057 . + . ID=gene-HOU18_gp41;Dbxref=GeneID:55003417;Name=HOU18_gp41;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp41;old_locus_tag=LL2_041 NC_048063.1 RefSeq CDS 26464 27057 . + 0 ID=cds-YP_009812353.1;Parent=gene-HOU18_gp41;Dbxref=Genbank:YP_009812353.1,GeneID:55003417;Name=YP_009812353.1;Note=Strong similarity to internal virion protein B of T3 and T7. InterPro results make the same predictions as matches to the canonical phage DB.;gbkey=CDS;locus_tag=HOU18_gp41;product=internal virion protein;protein_id=YP_009812353.1;transl_table=11 NC_048063.1 RefSeq gene 27050 29303 . + . ID=gene-HOU18_gp42;Dbxref=GeneID:55003418;Name=HOU18_gp42;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp42;old_locus_tag=LL2_042 NC_048063.1 RefSeq CDS 27060 29303 . + 0 ID=cds-YP_009812354.1;Parent=gene-HOU18_gp42;Dbxref=Genbank:YP_009812354.1,GeneID:55003418;Name=YP_009812354.1;Note=Strong matches to internal virion protein C of T3 and T7.%3B T3/T7-like internal virion protein;gbkey=CDS;locus_tag=HOU18_gp42;product=internal virion protein;protein_id=YP_009812354.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 27050 27054 . + . ID=id-NC_048063.1:27050..27054;Note=LL2_042;gbkey=regulatory;locus_tag=LL2_042;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 29312 33284 . + . ID=gene-HOU18_gp43;Dbxref=GeneID:55003419;Name=HOU18_gp43;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp43;old_locus_tag=LL2_043 NC_048063.1 RefSeq CDS 29322 33284 . + 0 ID=cds-YP_009812355.1;Parent=gene-HOU18_gp43;Dbxref=Genbank:YP_009812355.1,GeneID:55003419;Name=YP_009812355.1;Note=Strong matches to internal virion protein D of T3 and T7. Directly adjacent to predicted virion protein C.%3B InterPro results suggest that this gene contains a Transglycosylase SLT domain%2C a lysozyme-like domain%2C and Prokaryotic transglycosylases signature.%3B Gene seems to be T7 gp16-like. T7 gp16 is known to have Peptidoglycan hydrolytic activity.%3B T3/T7-like internal virion protein D;gbkey=CDS;locus_tag=HOU18_gp43;product=peptidoglycan hydrolase;protein_id=YP_009812355.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 29312 29317 . + . ID=id-HOU18_gp43;Dbxref=GeneID:55003419;Note=LL2_043;gbkey=regulatory;locus_tag=HOU18_gp43;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 33346 35098 . + . ID=gene-HOU18_gp44;Dbxref=GeneID:55003420;Name=HOU18_gp44;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp44;old_locus_tag=LL2_044 NC_048063.1 RefSeq CDS 33356 35098 . + 0 ID=cds-YP_009812356.1;Parent=gene-HOU18_gp44;Dbxref=Genbank:YP_009812356.1,GeneID:55003420;Name=YP_009812356.1;Note=BLAST hits to cannonical phage DB for tail fiber proteins of phage T3 and T7. Very strong BLAST (general) hit to Kluyvera phage Kvp1 for a tail fiber.%3B T3/T7-like tail fiber protein;gbkey=CDS;locus_tag=HOU18_gp44;product=tail fiber protein;protein_id=YP_009812356.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 33346 33350 . + . ID=id-HOU18_gp44;Dbxref=GeneID:55003420;Note=LL2_044;gbkey=regulatory;locus_tag=HOU18_gp44;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 35100 35635 . + . ID=gene-HOU18_gp45;Dbxref=GeneID:55003421;Name=HOU18_gp45;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp45;old_locus_tag=LL2_045 NC_048063.1 RefSeq CDS 35111 35635 . + 0 ID=cds-YP_009812357.1;Parent=gene-HOU18_gp45;Dbxref=Genbank:YP_009812357.1,GeneID:55003421;Name=YP_009812357.1;Note=Strong matches to at least 5 canonical phages (mostly lambdoid) for tail fiber assembly proteins.%3B InterPro result for T4-like tail fiber assembly.%3B Interestingly%2C this gene seems similar to a tape measure chaperone proteins found in siphophages.%3B lambdoid gpG-like;gbkey=CDS;locus_tag=HOU18_gp45;product=tail fiber assembly protein;protein_id=YP_009812357.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 35100 35105 . + . ID=id-HOU18_gp45;Dbxref=GeneID:55003421;Note=LL2_045;gbkey=regulatory;locus_tag=HOU18_gp45;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 35682 35898 . + . ID=gene-HOU18_gp46;Dbxref=GeneID:55003422;Name=HOU18_gp46;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp46;old_locus_tag=LL2_046 NC_048063.1 RefSeq CDS 35695 35898 . + 0 ID=cds-YP_009812358.1;Parent=gene-HOU18_gp46;Dbxref=Genbank:YP_009812358.1,GeneID:55003422;Name=YP_009812358.1;Note=Strong matches to T7 and T3 type II holin.%3B TMHMM detected a transmembrane protein in this gene. N out C in. This is expected in any phage holin protein.%3B T7/T3-like type II holin;gbkey=CDS;locus_tag=HOU18_gp46;product=type II holin;protein_id=YP_009812358.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 35682 35687 . + . ID=id-HOU18_gp46;Dbxref=GeneID:55003422;Note=LL2_046;gbkey=regulatory;locus_tag=HOU18_gp46;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 35891 36168 . + . ID=gene-HOU18_gp47;Dbxref=GeneID:55003423;Name=HOU18_gp47;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp47;old_locus_tag=LL2_047 NC_048063.1 RefSeq CDS 35902 36168 . + 0 ID=cds-YP_009812359.1;Parent=gene-HOU18_gp47;Dbxref=Genbank:YP_009812359.1,GeneID:55003423;Name=YP_009812359.1;Note=Strong similarty to DNA packaging proteins of T3 and T7. Interpro and Uniref exhibit similar results.%3B Multiple blast hits for various podoviridae phage terminases and DNA packaging proteins.%3B Upstream of putative large terminase subunit - this is the cannonical position of T7 TerS.%3B T3/T7-like DNA packaging protein;gbkey=CDS;locus_tag=HOU18_gp47;product=terminase small subunit;protein_id=YP_009812359.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 35891 35895 . + . ID=id-NC_048063.1:35891..35895;Note=LL2_047;gbkey=regulatory;locus_tag=LL2_047;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 36250 36711 . + . ID=gene-HOU18_gp48;Dbxref=GeneID:55003424;Name=HOU18_gp48;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp48;old_locus_tag=LL2_048 NC_048063.1 RefSeq CDS 36259 36711 . + 0 ID=cds-YP_009812360.1;Parent=gene-HOU18_gp48;Dbxref=Genbank:YP_009812360.1,GeneID:55003424;Name=YP_009812360.1;Note=Strong similarty to phage lambda Rz-like lysis protein in phages T3 and T7. Many BLAST hits in general are for lysis proteins.%3B The Rz-lysis protein typically consistutes the inner membrane spanin with an embedded outer membrane spanin.%3B A putative gene with a strong RBS ~9nt upstream of an ATG start codon is found embedded (predicted to be o-spanin).%3B Rz-like lysis protein;gbkey=CDS;locus_tag=HOU18_gp48;product=i-spanin;protein_id=YP_009812360.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 36250 36253 . + . ID=id-HOU18_gp48;Dbxref=GeneID:55003424;Note=LL2_048;gbkey=regulatory;locus_tag=HOU18_gp48;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 36359 36628 . + . ID=gene-HOU18_gp49;Dbxref=GeneID:55003425;Name=HOU18_gp49;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp49;old_locus_tag=LL2_049 NC_048063.1 RefSeq CDS 36374 36628 . + 0 ID=cds-YP_009812361.1;Parent=gene-HOU18_gp49;Dbxref=Genbank:YP_009812361.1,GeneID:55003425;Name=YP_009812361.1;Note=Embedded into another gene that is predicted to be an Rz-like lysis gene (o-spanin) . This is consistent with lambda Rz/Rz1-like spanin compelxes.%3B Highly similar to NP_042009.1 (T7p56)%2C an rz1 like protein%3B Rz1-like lysis protein;gbkey=CDS;locus_tag=HOU18_gp49;product=o-spanin;protein_id=YP_009812361.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 36359 36365 . + . ID=id-HOU18_gp49;Dbxref=GeneID:55003425;Note=LL2_049;gbkey=regulatory;locus_tag=HOU18_gp49;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 36674 38449 . + . ID=gene-HOU18_gp50;Dbxref=GeneID:55003426;Name=HOU18_gp50;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp50;old_locus_tag=LL2_050 NC_048063.1 RefSeq CDS 36686 38449 . + 0 ID=cds-YP_009812362.1;Parent=gene-HOU18_gp50;Dbxref=Genbank:YP_009812362.1,GeneID:55003426;Name=YP_009812362.1;Note=Strong homology to T3 DNA packaging protein B and T7 DNA maturation protein.%3B Strong homology to phage SP6p41 large terminase subunit.%3B Multiple blast hits for various podoviridae phage terminases and DNA packaging proteins.%3B T3/T7-like DNA packaging protein;gbkey=CDS;locus_tag=HOU18_gp50;product=terminase large subunit;protein_id=YP_009812362.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 36674 36678 . + . ID=id-NC_048063.1:36674..36678;Note=LL2_050;gbkey=regulatory;locus_tag=LL2_050;regulatory_class=ribosome_binding_site NC_048063.1 RefSeq gene 38682 38844 . + . ID=gene-HOU18_gp51;Dbxref=GeneID:55003427;Name=HOU18_gp51;gbkey=Gene;gene_biotype=protein_coding;locus_tag=HOU18_gp51;old_locus_tag=LL2_051 NC_048063.1 RefSeq CDS 38695 38844 . + 0 ID=cds-YP_009812363.1;Parent=gene-HOU18_gp51;Dbxref=Genbank:YP_009812363.1,GeneID:55003427;Name=YP_009812363.1;Note=Matches hypothetical proteins in T3 and T7. TMHMM detected a N in C out transmembrane protein.%3B T7 gp19.5-like.%3B Contains a transmembrane domain. T7 gp19.5.;gbkey=CDS;locus_tag=HOU18_gp51;product=putative transmembrane protein;protein_id=YP_009812363.1;transl_table=11 NC_048063.1 RefSeq ribosome_entry_site 38682 38688 . + . ID=id-HOU18_gp51;Dbxref=GeneID:55003427;Note=LL2_051;gbkey=regulatory;locus_tag=HOU18_gp51;regulatory_class=ribosome_binding_site ###