locus-tag	class	description
EWF20_RS00030	COG0630	type IV secretory pathway, VirB11 components, and related ATPases involved in Archaeal flagella biosynthesis
EWF20_RS00080	COG0601	transmembrane transport
EWF20_RS00085	COG0444	Belongs to the ABC transporter superfamily
EWF20_RS00090	COG4608	Belongs to the ABC transporter superfamily
EWF20_RS00110	COG0601	transmembrane transport
EWF20_RS00115	COG1173	ABC-type dipeptide oligopeptide nickel transport systems, permease components
EWF20_RS00120	COG0444	Belongs to the ABC transporter superfamily
EWF20_RS00125	COG4608	Belongs to the ABC transporter superfamily
EWF20_RS00135	COG0449	glutamine-fructose-6-phosphate transaminase (isomerizing) activity
EWF20_RS00190	COG2897	thiosulfate sulfurtransferase activity
EWF20_RS00200	COG0155	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family
EWF20_RS00205	COG0175	sulfate reduction
EWF20_RS00240	COG1249	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family
EWF20_RS00425	COG2329	Antibiotic biosynthesis monooxygenase
EWF20_RS00445	COG1063	alcohol dehydrogenase
EWF20_RS00460	COG1826	Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes
EWF20_RS00520	COG2223	nitrite transmembrane transporter activity
EWF20_RS00525	COG5013	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family
EWF20_RS00530	COG1140	nitrate reductase beta subunit
EWF20_RS00540	COG2181	nitrate reductase activity
EWF20_RS00710	COG1337	RAMP superfamily
EWF20_RS00760	COG0477	Major facilitator Superfamily
EWF20_RS00765	COG0013	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain
EWF20_RS00810	COG1078	dGTPase activity
EWF20_RS00945	COG0515	serine threonine protein kinase
EWF20_RS00945	COG2815	serine threonine protein kinase
EWF20_RS00950	COG1770	oligopeptidase
EWF20_RS00995	COG0365	Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA
EWF20_RS01010	COG1064	alcohol dehydrogenase
EWF20_RS01035	COG2813	Specifically methylates the guanine in position
EWF20_RS01055	COG0318	PFAM AMP-dependent synthetase and ligase
EWF20_RS01065	COG0764	3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity
EWF20_RS01070	COG1804	L-carnitine dehydratase bile acid-inducible protein F
EWF20_RS01075	COG1012	belongs to the aldehyde dehydrogenase family
EWF20_RS01085	COG0318	PFAM AMP-dependent synthetase and ligase
EWF20_RS01100	COG0365	Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA
EWF20_RS01115	COG4689	acetoacetate decarboxylase
EWF20_RS01120	COG0365	Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA
EWF20_RS01130	COG2271	Major facilitator Superfamily
EWF20_RS01135	COG4948	Mandelate Racemase Muconate Lactonizing
EWF20_RS01140	COG0318	PFAM AMP-dependent synthetase and ligase
EWF20_RS01145	COG2030	dehydratase
EWF20_RS01155	COG0183	Belongs to the thiolase family
EWF20_RS01170	COG0491	Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid
EWF20_RS01180	COG1960	acyl-CoA dehydrogenase activity
EWF20_RS01185	COG0095	Lipoate-protein ligase
EWF20_RS01195	COG1024	Enoyl-CoA hydratase
EWF20_RS01215	COG1250	3-hydroxyacyl-CoA dehydrogenase
EWF20_RS01240	COG0365	Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA
EWF20_RS01250	COG0145	ligase activity
EWF20_RS01285	COG1250	3-hydroxyacyl-CoA dehydrogenase
EWF20_RS01290	COG1028	Short-chain dehydrogenase reductase Sdr
EWF20_RS01300	COG1309	transcriptional regulator
EWF20_RS01310	COG1063	alcohol dehydrogenase
EWF20_RS01315	COG1960	acyl-CoA dehydrogenase activity
EWF20_RS01320	COG0318	PFAM AMP-dependent synthetase and ligase
EWF20_RS01325	COG0183	Belongs to the thiolase family
EWF20_RS01335	COG3383	formate dehydrogenase (NAD+) activity
EWF20_RS01345	COG1526	Required for formate dehydrogenase (FDH) activity. Acts as a sulfur carrier protein that transfers sulfur from IscS to the molybdenum cofactor prior to its insertion into FDH
EWF20_RS01350	COG1960	acyl-CoA dehydrogenase activity
EWF20_RS01360	COG1960	acyl-CoA dehydrogenase activity
EWF20_RS01385	COG2271	Major facilitator Superfamily
EWF20_RS01395	COG1529	Aldehyde oxidase and xanthine dehydrogenase
EWF20_RS01405	COG1024	Enoyl-CoA hydratase
EWF20_RS01410	COG0183	Belongs to the thiolase family
EWF20_RS01440	COG0843	Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1- 3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B
EWF20_RS01440	COG1845	cytochrome c oxidase, subunit III
EWF20_RS01470	COG1290	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis
EWF20_RS01485	COG0456	acetyltransferase
EWF20_RS01490	COG0281	malic enzyme
EWF20_RS01500	COG1832	CoA-binding protein
EWF20_RS01550	COG0535	radical SAM domain protein
EWF20_RS01555	COG0656	L-ascorbic acid biosynthetic process
EWF20_RS01595	COG1290	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis
EWF20_RS01630	COG0513	Belongs to the DEAD box helicase family
EWF20_RS01675	COG0174	glutamine synthetase
EWF20_RS01710	COG0079	Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily
EWF20_RS01720	COG0013	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain
EWF20_RS01745	COG1280	lysine exporter protein (LysE YggA)
EWF20_RS01765	COG0489	protein tyrosine kinase activity
EWF20_RS01815	COG2518	Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and or degradation of damaged proteins
EWF20_RS01820	COG0730	response to heat
EWF20_RS01840	COG0451	ADP-glyceromanno-heptose 6-epimerase activity
EWF20_RS01865	COG0020	Catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic pyrophosphates generating different type of terpenoids
EWF20_RS01870	COG0624	Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bacterial cell walls
EWF20_RS01875	COG4992	N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity
EWF20_RS01880	COG0189	ligase activity
EWF20_RS01895	COG0548	Belongs to the acetylglutamate kinase family. ArgB subfamily
EWF20_RS01900	COG0002	Catalyzes the NADPH-dependent reduction of N-acetyl-5- glutamyl phosphate to yield N-acetyl-L-glutamate 5-semialdehyde
EWF20_RS01915	COG0794	Belongs to the SIS family. GutQ KpsF subfamily
EWF20_RS01950	COG0028	Belongs to the TPP enzyme family
EWF20_RS01975	COG0017	Asparaginyl-tRNA synthetase
EWF20_RS01980	COG0585	pseudouridine synthase activity
EWF20_RS02000	COG0465	Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins
EWF20_RS02020	COG0373	Catalyzes the NADPH-dependent reduction of glutamyl- tRNA(Glu) to glutamate 1-semialdehyde (GSA)
EWF20_RS02025	COG0113	porphobilinogen synthase activity
EWF20_RS02030	COG0001	glutamate-1-semialdehyde 2,1-aminomutase activity
EWF20_RS02035	COG0181	hydroxymethylbilane synthase activity
EWF20_RS02060	COG0665	tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase activity
EWF20_RS02060	COG2022	thiamine diphosphate biosynthetic process
EWF20_RS02070	COG1793	dna ligase
EWF20_RS02075	COG0717	Belongs to the dCTP deaminase family
EWF20_RS02090	COG2078	PFAM AMMECR1 domain protein
EWF20_RS02120	COG1812	S-adenosylmethionine synthetase (AdoMet synthetase)
EWF20_RS02135	COG0504	CTP synthase activity
EWF20_RS02140	COG0269	3-dehydro-L-gulonate-6-phosphate decarboxylase activity
EWF20_RS02160	COG1109	Catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate
EWF20_RS02170	COG3830	Belongs to the UPF0237 family
EWF20_RS02175	COG2848	UPF0210 protein
EWF20_RS02330	COG0051	transcription antitermination factor activity, RNA binding
EWF20_RS02335	COG0050	This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis
EWF20_RS02340	COG0049	tRNA binding
EWF20_RS02360	COG1358	cellular component organization or biogenesis
EWF20_RS02365	COG0086	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates
EWF20_RS02370	COG0086	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates
EWF20_RS02375	COG0085	RNA polymerase activity
EWF20_RS02385	COG0281	malic enzyme
EWF20_RS02390	COG0532	One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex
EWF20_RS02395	COG0105	UTP biosynthetic process
EWF20_RS02410	COG0035	Catalyzes the conversion of uracil and 5-phospho-alpha- D-ribose 1-diphosphate (PRPP) to UMP and diphosphate
EWF20_RS02415	COG0064	Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln)
EWF20_RS02420	COG0503	Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis
EWF20_RS02430	COG1985	Converts 2,5-diamino-6-(ribosylamino)-4(3h)-pyrimidinone 5'-phosphate into 5-amino-6-(ribosylamino)-2,4(1h,3h)- pyrimidinedione 5'-phosphate
EWF20_RS02435	COG2896	Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8- dihydroguanosine 5'-triphosphate
EWF20_RS02450	COG0015	Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily
EWF20_RS02465	COG0104	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP
EWF20_RS02480	COG0077	Prephenate dehydratase
EWF20_RS02485	COG1171	Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short- lived. The second step is the nonenzymatic hydrolysis of the enamine imine intermediates to form 2-ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA
EWF20_RS02520	COG1051	belongs to the nudix hydrolase family
EWF20_RS02520	COG1056	cytidyltransferase-related domain
EWF20_RS02525	COG1607	acyl-coa hydrolase
EWF20_RS02630	COG1121	(ABC) transporter
EWF20_RS02635	COG1980	fructose-bisphosphate aldolase activity
EWF20_RS02660	COG0459	protein refolding
EWF20_RS02675	COG0124	histidyl-tRNA synthetase
EWF20_RS02695	COG0343	queuine tRNA-ribosyltransferase activity
EWF20_RS02710	COG0465	Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins
EWF20_RS02720	COG2262	GTPase that associates with the 50S ribosomal subunit and may have a role during protein synthesis or ribosome biogenesis
EWF20_RS02765	COG0499	May play a key role in the regulation of the intracellular concentration of adenosylhomocysteine
EWF20_RS02775	COG0477	Major facilitator Superfamily
EWF20_RS02795	COG1236	Exonuclease of the beta-lactamase fold involved in RNA processing
EWF20_RS02805	COG1047	Peptidyl-prolyl cis-trans
EWF20_RS02815	COG0421	Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy- AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine
EWF20_RS02880	COG1131	(ABC) transporter
EWF20_RS02905	COG1136	(ABC) transporter
EWF20_RS02905	COG0577	efflux transmembrane transporter activity
EWF20_RS02910	COG1136	(ABC) transporter
EWF20_RS02925	COG3383	formate dehydrogenase (NAD+) activity
EWF20_RS02945	COG0023	Translation Initiation Factor
EWF20_RS02960	COG0423	glycine-tRNA ligase activity
EWF20_RS02990	COG0163	prenyltransferase activity
EWF20_RS03000	COG1635	Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur
EWF20_RS03015	COG0533	Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction
EWF20_RS03025	COG0127	Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA RNA and avoiding chromosomal lesions
EWF20_RS03030	COG0667	Aldo Keto reductase
EWF20_RS03075	COG1110	DNA topoisomerase II activity
EWF20_RS03080	COG0465	Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins
EWF20_RS03085	COG3635	2,3-bisphosphoglycerate-independent phosphoglycerate mutase activity
EWF20_RS03130	COG0620	Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation
EWF20_RS03135	COG0620	Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation
EWF20_RS03165	COG0054	6,7-dimethyl-8-ribityllumazine synthase activity
EWF20_RS03195	COG1201	RNA secondary structure unwinding
EWF20_RS03205	COG0130	Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs
EWF20_RS03210	COG0130	Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs
EWF20_RS03225	COG0438	transferase activity, transferring glycosyl groups
EWF20_RS03320	COG0283	belongs to the cytidylate kinase family. Type 1 subfamily
EWF20_RS03345	COG0200	Binds to the 23S rRNA
EWF20_RS03380	COG0096	One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit
EWF20_RS03405	COG0093	Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome
EWF20_RS03420	COG0255	Belongs to the universal ribosomal protein uL29 family
EWF20_RS03425	COG0092	Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation
EWF20_RS03430	COG0091	The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome
EWF20_RS03435	COG0185	Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA
EWF20_RS03440	COG0090	One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity
EWF20_RS03445	COG0089	rRNA binding
EWF20_RS03450	COG0088	Forms part of the polypeptide exit tunnel
EWF20_RS03455	COG0087	One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit
EWF20_RS03470	COG0473	3-isopropylmalate dehydrogenase activity
EWF20_RS03490	COG0480	Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome
EWF20_RS03500	COG0322	excinuclease ABC activity
EWF20_RS03530	COG1185	polyribonucleotide nucleotidyltransferase activity
EWF20_RS03570	COG0012	GTP binding
EWF20_RS03575	COG1209	Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis
EWF20_RS03585	COG0565	Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA
EWF20_RS03590	COG2264	protein methyltransferase activity
EWF20_RS03595	COG0030	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity
EWF20_RS03650	COG0125	Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis
EWF20_RS03710	COG0315	cyclic pyranopterin monophosphate synthase activity
EWF20_RS03715	COG2256	atpase related to the helicase subunit of the holliday junction resolvase
EWF20_RS03725	COG1881	PFAM PEBP family protein
EWF20_RS03730	COG0638	Catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation. This tagging system is termed pupylation. The ligation reaction involves the side-chain carboxylate of the C-terminal glutamate of Pup and the side-chain amino group of a substrate lysine
EWF20_RS03735	COG0154	amidase activity
EWF20_RS03745	COG1063	alcohol dehydrogenase
EWF20_RS03770	COG2236	Acts on guanine, xanthine and to a lesser extent hypoxanthine
EWF20_RS03775	COG0005	The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta- (deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate
EWF20_RS03790	COG0189	ligase activity
EWF20_RS03795	COG1522	sequence-specific DNA binding
EWF20_RS03825	COG0644	oxidoreductase
EWF20_RS03845	COG1053	succinate dehydrogenase
EWF20_RS03855	COG2048	Heterodisulfide reductase, subunit B
EWF20_RS03870	COG0714	Associated with various cellular activities
EWF20_RS03885	COG0303	'Molybdopterin
EWF20_RS03890	COG0083	homoserine kinase activity
EWF20_RS03895	COG0626	cystathionine gamma-synthase activity
EWF20_RS03935	COG0183	Belongs to the thiolase family
EWF20_RS03940	COG0621	Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine
EWF20_RS03980	COG1215	transferase activity, transferring glycosyl groups
EWF20_RS03995	COG0351	phosphomethylpyrimidine kinase
EWF20_RS04000	COG0314	molybdopterin synthase activity
EWF20_RS04065	COG0119	Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)
EWF20_RS04120	COG0465	Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins
EWF20_RS04135	COG2236	Acts on guanine, xanthine and to a lesser extent hypoxanthine
EWF20_RS04140	COG2185	Catalyzes the reversible interconversion of isobutyryl- CoA and n-butyryl-CoA, using radical chemistry. Also exhibits GTPase activity, associated with its G-protein domain (MeaI) that functions as a chaperone that assists cofactor delivery and proper holo-enzyme assembly
EWF20_RS04140	COG1884	Catalyzes the reversible interconversion of isobutyryl- CoA and n-butyryl-CoA, using radical chemistry. Also exhibits GTPase activity, associated with its G-protein domain (MeaI) that functions as a chaperone that assists cofactor delivery and proper holo-enzyme assembly
EWF20_RS04145	COG0346	lactoylglutathione lyase activity
EWF20_RS04150	COG0071	Belongs to the small heat shock protein (HSP20) family
EWF20_RS04175	COG1207	Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP- GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5- monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain
EWF20_RS04195	COG1648	precorrin-2 dehydrogenase activity
EWF20_RS04195	COG0007	Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD- dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme
EWF20_RS04240	COG0430	RNA-3'-phosphate cyclase activity
EWF20_RS04250	COG0389	Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII
EWF20_RS04255	COG0524	Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5- phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway
EWF20_RS04260	COG0465	Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins
EWF20_RS04265	COG1051	belongs to the nudix hydrolase family
EWF20_RS04270	COG0518	GMP synthase (glutamine-hydrolyzing) activity
EWF20_RS04270	COG0519	Catalyzes the synthesis of GMP from XMP
EWF20_RS04275	COG0467	Core component of the KaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria. Binds to DNA. The KaiABC complex may act as a promoter-nonspecific transcription regulator that represses transcription, possibly by acting on the state of chromosome compaction
EWF20_RS04280	COG5599	Phosphatase
EWF20_RS04285	COG1515	deoxyribonuclease V activity
EWF20_RS04290	COG1335	isochorismatase
EWF20_RS04300	COG0450	alkyl hydroperoxide reductase
EWF20_RS04315	COG0372	Belongs to the citrate synthase family
EWF20_RS04340	COG4799	Acetyl-CoA carboxylase, carboxyltransferase component subunits alpha and beta
EWF20_RS04350	COG0439	Biotin carboxylase
EWF20_RS04360	COG0715	COG0715 ABC-type nitrate sulfonate bicarbonate transport systems periplasmic components
EWF20_RS04360	COG1116	anion transmembrane transporter activity
EWF20_RS04365	COG1826	Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes
EWF20_RS04375	COG0805	protein transport
EWF20_RS04380	COG0065	Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate
EWF20_RS04385	COG0066	3-isopropylmalate dehydratase activity
EWF20_RS04390	COG0778	Nitroreductase
EWF20_RS04420	COG1212	Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria
EWF20_RS04430	COG1131	(ABC) transporter
EWF20_RS04445	COG0843	Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1- 3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B
EWF20_RS04520	COG0495	leucyl-tRNA aminoacylation
EWF20_RS04530	COG0624	Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bacterial cell walls
EWF20_RS04550	COG0037	tRNA processing
EWF20_RS04560	COG0846	NAD-dependent lysine deacetylase and desuccinylase that specifically removes acetyl and succinyl groups on target proteins. Modulates the activities of several proteins which are inactive in their acylated form
EWF20_RS04590	COG0475	glutathione-regulated potassium exporter activity
EWF20_RS04595	COG0243	molybdopterin cofactor binding
EWF20_RS04630	COG0345	Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline
EWF20_RS04640	COG0669	Reversibly transfers an adenylyl group from ATP to 4'- phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate
EWF20_RS04645	COG0277	FAD linked oxidase domain protein
EWF20_RS04655	COG1252	NADH dehydrogenase
EWF20_RS04700	COG0004	ammonium transporteR
EWF20_RS04725	COG2814	Major facilitator Superfamily
EWF20_RS04740	COG0584	glycerophosphoryl diester phosphodiesterase
EWF20_RS04765	COG0491	Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid
EWF20_RS04770	COG0843	Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1- 3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B
EWF20_RS04800	COG0144	Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA
EWF20_RS04820	COG1249	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family
EWF20_RS04880	COG0778	Nitroreductase
EWF20_RS04885	COG1080	General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr)
EWF20_RS04885	COG0574	Belongs to the PEP-utilizing enzyme family
EWF20_RS04900	COG4608	Belongs to the ABC transporter superfamily
EWF20_RS04905	COG0444	Belongs to the ABC transporter superfamily
EWF20_RS04910	COG1173	ABC-type dipeptide oligopeptide nickel transport systems, permease components
EWF20_RS04915	COG0601	transmembrane transport
EWF20_RS04930	COG2814	Major facilitator Superfamily
EWF20_RS04970	COG1121	(ABC) transporter
EWF20_RS05020	COG1225	Alkyl hydroperoxide reductase Thiol specific antioxidant Mal allergen
EWF20_RS05030	COG1853	conserved protein domain typically associated with flavoprotein oxygenases, DIM6 NTAB family
EWF20_RS05040	COG2368	4-hydroxyphenylacetate
EWF20_RS05050	COG0365	Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA
EWF20_RS05055	COG1584	GPR1 FUN34 yaaH family protein
EWF20_RS05060	COG4274	PFAM GYD family protein
EWF20_RS05075	COG4992	N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity
EWF20_RS05105	COG2807	transmembrane transport
EWF20_RS05205	COG2151	metal-sulfur cluster biosynthetic enzyme
EWF20_RS05250	COG3962	Belongs to the TPP enzyme family
EWF20_RS05270	COG4555	ABC transporter
EWF20_RS05345	COG0455	bacterial-type flagellum organization
EWF20_RS05350	COG0517	IMP dehydrogenase activity
EWF20_RS05365	COG2723	6-phospho-beta-galactosidase activity
EWF20_RS05375	COG2226	methyltransferase
EWF20_RS05390	COG0515	serine threonine protein kinase
EWF20_RS05395	COG4946	Tricorn protease homolog
EWF20_RS05395	COG1506	peptidase
EWF20_RS05395	COG0823	Involved in the tonB-independent uptake of proteins
EWF20_RS05410	COG0365	Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA
EWF20_RS05510	COG0041	Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR)
EWF20_RS05515	COG0026	Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR)
EWF20_RS05550	COG0517	IMP dehydrogenase activity
EWF20_RS05550	COG0516	GMP reductase activity
EWF20_RS05565	COG0448	Catalyzes the synthesis of ADP-glucose, a sugar donor used in elongation reactions on alpha-glucans
EWF20_RS05575	COG0297	glycogen (starch) synthase activity
EWF20_RS05580	COG2041	Part of the MsrPQ system that repairs oxidized periplasmic proteins containing methionine sulfoxide residues (Met-O), using respiratory chain electrons. Thus protects these proteins from oxidative-stress damage caused by reactive species of oxygen and chlorine generated by the host defense mechanisms. MsrPQ is essential for the maintenance of envelope integrity under bleach stress, rescuing a wide series of structurally unrelated periplasmic proteins from methionine oxidation. The catalytic subunit MsrP is non-stereospecific, being able to reduce both (R-) and (S-) diastereoisomers of methionine sulfoxide
EWF20_RS05595	COG2057	Acyl CoA acetate 3-ketoacid CoA transferase beta subunit
EWF20_RS05645	COG1048	Catalyzes the isomerization of citrate to isocitrate via cis-aconitate
EWF20_RS05660	COG0501	Belongs to the peptidase M48B family
EWF20_RS05700	COG0609	Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily
EWF20_RS05705	COG1121	(ABC) transporter
EWF20_RS05745	COG0519	Catalyzes the synthesis of GMP from XMP
EWF20_RS05780	COG0531	amino acid
EWF20_RS05790	COG0486	Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34
EWF20_RS05850	COG0491	Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid
EWF20_RS05915	COG0297	glycogen (starch) synthase activity
EWF20_RS05915	COG0438	transferase activity, transferring glycosyl groups
EWF20_RS05950	COG0693	protein deglycase activity
EWF20_RS05995	COG0577	efflux transmembrane transporter activity
EWF20_RS05995	COG1136	(ABC) transporter
EWF20_RS06005	COG0577	efflux transmembrane transporter activity
EWF20_RS06005	COG1136	(ABC) transporter
EWF20_RS06010	COG1520	Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane
EWF20_RS06050	COG0553	Transcription regulator that activates transcription by stimulating RNA polymerase (RNAP) recycling in case of stress conditions such as supercoiled DNA or high salt concentrations. Probably acts by releasing the RNAP, when it is trapped or immobilized on tightly supercoiled DNA. Does not activate transcription on linear DNA. Probably not involved in DNA repair
EWF20_RS06070	COG2124	cytochrome p450
EWF20_RS06075	COG1195	it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP
EWF20_RS06180	COG2264	protein methyltransferase activity
EWF20_RS06195	COG2518	Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and or degradation of damaged proteins
EWF20_RS06200	COG3387	PFAM Glycoside hydrolase 15-related
EWF20_RS06365	COG1312	Catalyzes the dehydration of D-mannonate
EWF20_RS06375	COG2030	dehydratase
EWF20_RS06380	COG1804	L-carnitine dehydratase bile acid-inducible protein F
EWF20_RS06385	COG1028	Short-chain dehydrogenase reductase Sdr
EWF20_RS06395	COG1172	Belongs to the binding-protein-dependent transport system permease family
EWF20_RS06440	COG4948	Mandelate Racemase Muconate Lactonizing
EWF20_RS06445	COG1028	Short-chain dehydrogenase reductase Sdr
EWF20_RS06465	COG0434	BtpA family
EWF20_RS06475	COG2814	Major facilitator Superfamily
EWF20_RS06485	COG0318	PFAM AMP-dependent synthetase and ligase
EWF20_RS06490	COG4992	N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity
EWF20_RS06500	COG0477	Major facilitator Superfamily
EWF20_RS06500	COG2814	Major facilitator Superfamily
EWF20_RS06505	COG0122	3-methyladenine DNA glycosylase 8-oxoguanine DNA glycosylase
EWF20_RS06535	COG0393	Putative heavy-metal-binding
EWF20_RS06580	COG1249	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family
EWF20_RS06615	COG1121	(ABC) transporter
EWF20_RS06670	COG1131	(ABC) transporter
EWF20_RS06845	COG1274	phosphoenolpyruvate carboxykinase (GTP) activity
EWF20_RS06855	COG1489	DNA binding
EWF20_RS06880	COG0455	bacterial-type flagellum organization
EWF20_RS06990	COG1733	regulation of RNA biosynthetic process
EWF20_RS07015	COG0298	carbon dioxide binding
EWF20_RS07040	COG0298	carbon dioxide binding
EWF20_RS07050	COG0374	Belongs to the NiFe NiFeSe hydrogenase large subunit family
EWF20_RS07060	COG3376	Belongs to the NiCoT transporter (TC 2.A.52) family
EWF20_RS07100	COG3511	phospholipase C
EWF20_RS07110	COG1122	ATPase activity
EWF20_RS07165	COG2235	arginine
EWF20_RS07170	COG1017	nitric oxide dioxygenase activity
EWF20_RS07180	COG2223	nitrite transmembrane transporter activity
EWF20_RS07195	COG0243	molybdopterin cofactor binding
EWF20_RS07210	COG0804	urea catabolic process
EWF20_RS07225	COG0378	Facilitates the functional incorporation of the urease nickel metallocenter. This process requires GTP hydrolysis, probably effectuated by UreG
EWF20_RS07235	COG1513	Catalyzes the reaction of cyanate with bicarbonate to produce ammonia and carbon dioxide
EWF20_RS07245	COG0388	Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source
EWF20_RS07255	COG0589	response to stress
EWF20_RS07330	COG2080	2 iron, 2 sulfur cluster binding
EWF20_RS07330	COG1529	Aldehyde oxidase and xanthine dehydrogenase
EWF20_RS07350	COG0044	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides
EWF20_RS07380	COG2041	Part of the MsrPQ system that repairs oxidized periplasmic proteins containing methionine sulfoxide residues (Met-O), using respiratory chain electrons. Thus protects these proteins from oxidative-stress damage caused by reactive species of oxygen and chlorine generated by the host defense mechanisms. MsrPQ is essential for the maintenance of envelope integrity under bleach stress, rescuing a wide series of structurally unrelated periplasmic proteins from methionine oxidation. The catalytic subunit MsrP is non-stereospecific, being able to reduce both (R-) and (S-) diastereoisomers of methionine sulfoxide
EWF20_RS07390	COG0383	alpha-mannosidase activity
EWF20_RS07395	COG0352	thiamine-phosphate diphosphorylase activity
EWF20_RS07395	COG0819	Catalyzes an amino-pyrimidine hydrolysis reaction at the C5' of the pyrimidine moiety of thiamine compounds, a reaction that is part of a thiamine salvage pathway
EWF20_RS07395	COG0351	phosphomethylpyrimidine kinase
EWF20_RS07400	COG0477	Major facilitator Superfamily
EWF20_RS07450	COG0066	3-isopropylmalate dehydratase activity
EWF20_RS07455	COG0065	Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate
EWF20_RS07500	COG0454	-acetyltransferase
EWF20_RS07590	COG1078	dGTPase activity
EWF20_RS07595	COG0813	purine-nucleoside phosphorylase activity
EWF20_RS07605	COG0438	transferase activity, transferring glycosyl groups
EWF20_RS07610	COG3387	PFAM Glycoside hydrolase 15-related
EWF20_RS07620	COG1252	NADH dehydrogenase
EWF20_RS07665	COG1208	COG1208 Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis translation initiation factor 2B, gamma epsilon subunits eIF-2Bgamma eIF-2Bepsilon
EWF20_RS07675	COG0441	Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr)
EWF20_RS07685	COG0177	DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N- glycosidic bond, leaving an AP (apurinic apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
EWF20_RS07690	COG0045	succinate-CoA ligase (ADP-forming) activity
EWF20_RS07730	COG0243	molybdopterin cofactor binding
EWF20_RS07750	COG0309	Hydrogenase expression formation protein (HypE)
EWF20_RS07760	COG0409	Hydrogenase expression formation protein
EWF20_RS07850	COG1514	Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'- phosphomonoester
EWF20_RS07875	COG0462	Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib-5-P)
EWF20_RS07910	COG1185	polyribonucleotide nucleotidyltransferase activity
EWF20_RS07935	COG0300	oxidoreductase activity
EWF20_RS07940	COG0084	hydrolase, TatD family'
EWF20_RS07950	COG2814	Major facilitator Superfamily
EWF20_RS08015	COG0182	Catalyzes the interconversion of methylthioribose-1- phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1- P)
EWF20_RS08025	COG0265	Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain
EWF20_RS08035	COG1960	acyl-CoA dehydrogenase activity
EWF20_RS08060	COG2814	Major facilitator Superfamily
EWF20_RS08075	COG4946	Tricorn protease homolog
EWF20_RS08075	COG0793	Belongs to the peptidase S41A family
EWF20_RS08085	COG2271	Major facilitator Superfamily
EWF20_RS08095	COG0630	type IV secretory pathway, VirB11 components, and related ATPases involved in Archaeal flagella biosynthesis
EWF20_RS08190	COG0365	Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA
EWF20_RS08220	COG0029	Catalyzes the oxidation of L-aspartate to iminoaspartate
EWF20_RS08225	COG0379	Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate
EWF20_RS08250	COG0719	Fe-S assembly protein
EWF20_RS08255	COG0396	ATPase activity
EWF20_RS08275	COG0509	glycine decarboxylation via glycine cleavage system
EWF20_RS08280	COG0404	The glycine cleavage system catalyzes the degradation of glycine
EWF20_RS08285	COG0403	The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor
EWF20_RS08290	COG1003	The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor
EWF20_RS08305	COG0148	phosphopyruvate hydratase activity
EWF20_RS08325	COG0465	Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins
EWF20_RS08330	COG0550	Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone
EWF20_RS08345	COG1052	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family
EWF20_RS08350	COG1059	Responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine 7-oxoG) from DNA. Also nicks DNA at apurinic apyrimidinic sites (AP sites)
EWF20_RS08380	COG0525	valine-tRNA ligase activity
EWF20_RS08385	COG0436	Aminotransferase
EWF20_RS08390	COG0134	indole-3-glycerol-phosphate synthase activity
EWF20_RS08395	COG0512	Glutamine amidotransferase of anthranilate synthase
EWF20_RS08395	COG0547	Catalyzes the transfer of the phosphoribosyl group of 5- phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'-phosphoribosyl)-anthranilate (PRA)
EWF20_RS08400	COG0147	Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine- binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia
EWF20_RS08405	COG0135	phosphoribosylanthranilate isomerase activity
EWF20_RS08415	COG0159	tryptophan synthase activity
EWF20_RS08420	COG1350	The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine
EWF20_RS08425	COG1254	Belongs to the acylphosphatase family
EWF20_RS08440	COG1080	General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr)
EWF20_RS08440	COG0574	Belongs to the PEP-utilizing enzyme family
EWF20_RS08460	COG0498	Catalyzes the gamma-elimination of phosphate from L- phosphohomoserine and the beta-addition of water to produce L- threonine
EWF20_RS08465	COG0527	aspartate kinase activity
EWF20_RS08465	COG0460	homoserine dehydrogenase
EWF20_RS08475	COG0587	DNA-directed DNA polymerase activity
EWF20_RS08485	COG0078	Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline
EWF20_RS08520	COG0459	protein refolding
EWF20_RS08555	COG0209	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides
EWF20_RS08575	COG0252	asparaginase activity
EWF20_RS08605	COG1867	N(2),N(2)-dimethylguanosine tRNA methyltransferase
EWF20_RS08655	COG2870	Catalyzes the ADP transfer from ATP to D-glycero-beta-D- manno-heptose 1-phosphate, yielding ADP-D-glycero-beta-D-manno- heptose
EWF20_RS08655	COG0615	ADP-L-glycero-beta-D-manno-heptose biosynthetic process
EWF20_RS08670	COG0154	amidase activity
EWF20_RS08675	COG0721	Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln)
EWF20_RS08680	COG1324	tolerance protein
EWF20_RS08715	COG1110	DNA topoisomerase II activity
EWF20_RS08725	COG1201	RNA secondary structure unwinding
EWF20_RS08760	COG0231	Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase
EWF20_RS08765	COG0541	Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY
EWF20_RS08775	COG1028	Short-chain dehydrogenase reductase Sdr
EWF20_RS08795	COG0119	Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)
EWF20_RS08810	COG0120	Catalyzes the reversible conversion of ribose-5- phosphate to ribulose 5-phosphate
EWF20_RS08830	COG0535	radical SAM domain protein
EWF20_RS08850	COG1586	Catalyzes the decarboxylation of S-adenosylmethionine to S-adenosylmethioninamine (dcAdoMet), the propylamine donor required for the synthesis of the polyamines spermine and spermidine from the diamine putrescine
EWF20_RS08875	COG0112	Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF- independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism
EWF20_RS08880	COG0589	response to stress
EWF20_RS08890	COG0126	phosphoglycerate kinase activity
EWF20_RS08925	COG0013	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain
EWF20_RS08935	COG0244	Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors
EWF20_RS08945	COG0080	Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors
EWF20_RS08960	COG0552	protein localization to endoplasmic reticulum
EWF20_RS09025	COG0031	Belongs to the cysteine synthase cystathionine beta- synthase family
EWF20_RS09045	COG2896	Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8- dihydroguanosine 5'-triphosphate
EWF20_RS09050	COG0006	proline dipeptidase activity
EWF20_RS09055	COG0302	gtp cyclohydrolase
EWF20_RS09075	COG0318	PFAM AMP-dependent synthetase and ligase
EWF20_RS09090	COG0663	COG0663 Carbonic anhydrases acetyltransferases, isoleucine patch superfamily
EWF20_RS09125	COG2804	Type II secretory pathway, ATPase PulE Tfp pilus assembly pathway, ATPase PilB
EWF20_RS09160	COG1215	transferase activity, transferring glycosyl groups
EWF20_RS09165	COG1201	RNA secondary structure unwinding
EWF20_RS09170	COG0618	3'(2'),5'-bisphosphate nucleotidase activity
EWF20_RS09170	COG0617	tRNA 3'-terminal CCA addition
EWF20_RS09170	COG0517	IMP dehydrogenase activity
EWF20_RS09195	COG1167	Transcriptional regulators containing a DNA-binding HTH domain and an aminotransferase domain MocR family and their eukaryotic orthologs
EWF20_RS09200	COG1058	Belongs to the CinA family
EWF20_RS09200	COG1546	Belongs to the CinA family
EWF20_RS09205	COG2021	Transfers an acetyl group from acetyl-CoA to L- homoserine, forming acetyl-L-homoserine
EWF20_RS09220	COG2220	Zn-dependent hydrolases of the beta-lactamase fold
EWF20_RS09225	COG0024	Methionine aminopeptidase
EWF20_RS09250	COG0561	phosphatase activity
EWF20_RS09255	COG0008	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)
EWF20_RS09275	COG0417	DNA replication proofreading
EWF20_RS09305	COG0143	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation
EWF20_RS09325	COG1155	Produces ATP from ADP in the presence of a proton gradient across the membrane. The V-type alpha chain is a catalytic subunit
EWF20_RS09335	COG1394	ATPase activity, coupled to movement of substances
EWF20_RS09350	COG0442	prolyl-tRNA aminoacylation
EWF20_RS09360	COG0214	Catalyzes the formation of pyridoxal 5'-phosphate from ribose 5-phosphate (RBP), glyceraldehyde 3-phosphate (G3P) and ammonia. The ammonia is provided by the PdxT subunit. Can also use ribulose 5-phosphate and dihydroxyacetone phosphate as substrates, resulting from enzyme-catalyzed isomerization of RBP and G3P, respectively
EWF20_RS09365	COG0311	Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS
EWF20_RS09380	COG0059	Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol- acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3- dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3- hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate
EWF20_RS09390	COG0028	Belongs to the TPP enzyme family
EWF20_RS09400	COG1161	Required for a late step of 50S ribosomal subunit assembly. Has GTPase activity
EWF20_RS09410	COG0382	Catalyzes the prenylation of para-hydroxybenzoate (PHB) with an all-trans polyprenyl group. Mediates the second step in the final reaction sequence of ubiquinone-8 (UQ-8) biosynthesis, which is the condensation of the polyisoprenoid side chain with PHB, generating the first membrane-bound Q intermediate 3- octaprenyl-4-hydroxybenzoate
EWF20_RS09425	COG1586	Catalyzes the decarboxylation of S-adenosylmethionine to S-adenosylmethioninamine (dcAdoMet), the propylamine donor required for the synthesis of the polyamines spermine and spermidine from the diamine putrescine
EWF20_RS09435	COG0037	tRNA processing
EWF20_RS09445	COG0495	leucyl-tRNA aminoacylation
EWF20_RS09455	COG0079	Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily
EWF20_RS09460	COG0040	Catalyzes the condensation of ATP and 5-phosphoribose 1- diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of hisG enzymatic activity
EWF20_RS09470	COG0131	Imidazoleglycerol-phosphate dehydratase
EWF20_RS09475	COG0107	imidazoleglycerol-phosphate synthase activity
EWF20_RS09480	COG0141	Catalyzes the sequential NAD-dependent oxidations of L- histidinol to L-histidinaldehyde and then to L-histidine
EWF20_RS09485	COG0140	phosphoribosyl-ATP diphosphatase activity
EWF20_RS09490	COG0118	IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to HisF for the synthesis of IGP and AICAR
EWF20_RS09500	COG2151	metal-sulfur cluster biosynthetic enzyme
EWF20_RS09505	COG0172	Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec)
EWF20_RS09510	COG0621	Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine
EWF20_RS09520	COG0537	adenosine 5'-monophosphoramidase activity
EWF20_RS09530	COG1522	sequence-specific DNA binding
EWF20_RS09550	COG0167	dihydroorotate dehydrogenase activity
EWF20_RS09555	COG0044	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides
EWF20_RS09565	COG1781	'de novo' pyrimidine nucleobase biosynthetic process
EWF20_RS09570	COG0540	Belongs to the ATCase OTCase family
EWF20_RS09620	COG0602	queuosine metabolic process
EWF20_RS09625	COG0536	An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control
EWF20_RS09630	COG0073	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation
EWF20_RS09630	COG0143	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation
EWF20_RS09635	COG0152	phosphoribosylaminoimidazolesuccinocarboxamide synthase activity
EWF20_RS09645	COG0047	phosphoribosylformylglycinamidine synthase activity
EWF20_RS09650	COG0046	Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL
EWF20_RS09655	COG0034	Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine
EWF20_RS09660	COG0034	Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine
EWF20_RS09670	COG0150	phosphoribosylformylglycinamidine cyclo-ligase activity
EWF20_RS09685	COG0137	argininosuccinate synthase activity
EWF20_RS09695	COG0505	Belongs to the CarA family
EWF20_RS09705	COG0189	ligase activity
EWF20_RS09715	COG1250	3-hydroxyacyl-CoA dehydrogenase
EWF20_RS09760	COG1024	Enoyl-CoA hydratase
EWF20_RS09770	COG1612	heme a metabolic process
EWF20_RS09770	COG0109	protoheme IX farnesyltransferase activity
EWF20_RS09775	COG0460	homoserine dehydrogenase
EWF20_RS09790	COG0312	modulator of DNA gyrase
EWF20_RS09800	COG0589	response to stress
EWF20_RS09810	COG0009	L-threonylcarbamoyladenylate synthase
EWF20_RS09815	COG0377	NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient
EWF20_RS09845	COG0674	pyruvate-flavodoxin oxidoreductase activity
EWF20_RS09850	COG1013	pyruvate-flavodoxin oxidoreductase activity
EWF20_RS09915	COG0125	Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis
EWF20_RS09920	COG0125	Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis
EWF20_RS09930	COG2062	phosphohistidine phosphatase, SixA
EWF20_RS09935	COG0501	Belongs to the peptidase M48B family
EWF20_RS09945	COG0491	Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid
EWF20_RS09965	COG0437	4 iron, 4 sulfur cluster binding
EWF20_RS09970	COG0243	molybdopterin cofactor binding
EWF20_RS10075	COG1350	The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine
EWF20_RS10205	COG2220	Zn-dependent hydrolases of the beta-lactamase fold
EWF20_RS10220	COG1131	(ABC) transporter
EWF20_RS10265	COG1195	it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP
EWF20_RS10330	COG1373	ATPase (AAA superfamily
EWF20_RS10465	COG2192	nodulation
EWF20_RS10515	COG1442	glycosyl transferase family 8
EWF20_RS10515	COG1216	Glycosyl transferase, family 2
EWF20_RS10555	COG1216	Glycosyl transferase, family 2
EWF20_RS10585	COG1078	dGTPase activity
EWF20_RS10765	COG0465	Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins
EWF20_RS10775	COG2814	Major facilitator Superfamily
EWF20_RS10790	COG1137	ATPase activity
EWF20_RS10795	COG0179	Fumarylacetoacetate (FAA) hydrolase
EWF20_RS10940	COG0417	DNA replication proofreading
EWF20_RS10990	COG2814	Major facilitator Superfamily
EWF20_RS11005	COG2329	Antibiotic biosynthesis monooxygenase
EWF20_RS11010	COG1290	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis
EWF20_RS11070	COG1139	Iron-sulfur cluster binding protein
EWF20_RS11085	COG0661	Is probably a protein kinase regulator of UbiI activity which is involved in aerobic coenzyme Q (ubiquinone) biosynthesis
EWF20_RS11095	COG2317	Broad specificity carboxypetidase that releases amino acids sequentially from the C-terminus, including neutral, aromatic, polar and basic residues
EWF20_RS11100	COG0601	transmembrane transport
EWF20_RS11205	COG1012	belongs to the aldehyde dehydrogenase family
EWF20_RS11220	COG3350	monooxygenase activity
EWF20_RS11230	COG2146	nitrite reductase [NAD(P)H] activity
EWF20_RS11245	COG2151	metal-sulfur cluster biosynthetic enzyme
EWF20_RS11250	COG0028	Belongs to the TPP enzyme family
EWF20_RS11265	COG1071	oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor
EWF20_RS11270	COG0022	dehydrogenase e1 component
EWF20_RS11330	COG0665	tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase activity
EWF20_RS11350	COG2226	methyltransferase
EWF20_RS11390	COG1200	Critical role in recombination and DNA repair. Helps process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-DNA)
EWF20_RS11420	COG2860	membrane
EWF20_RS11440	COG0498	Catalyzes the gamma-elimination of phosphate from L- phosphohomoserine and the beta-addition of water to produce L- threonine
EWF20_RS11590	COG1131	(ABC) transporter
EWF20_RS11610	COG0644	oxidoreductase
EWF20_RS11615	COG2025	fatty acid beta-oxidation using acyl-CoA dehydrogenase
EWF20_RS11635	COG2897	thiosulfate sulfurtransferase activity
EWF20_RS11640	COG1225	Alkyl hydroperoxide reductase Thiol specific antioxidant Mal allergen
EWF20_RS11650	COG1013	pyruvate-flavodoxin oxidoreductase activity
EWF20_RS11655	COG0674	pyruvate-flavodoxin oxidoreductase activity
EWF20_RS11670	COG3383	formate dehydrogenase (NAD+) activity
EWF20_RS11685	COG1960	acyl-CoA dehydrogenase activity
EWF20_RS11690	COG2086	electron transfer activity
EWF20_RS11700	COG0644	oxidoreductase
EWF20_RS11725	COG0332	Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched- chain and or straight-chain of fatty acids
EWF20_RS11745	COG1960	acyl-CoA dehydrogenase activity
EWF20_RS11750	COG0365	Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA
EWF20_RS11760	COG0372	Belongs to the citrate synthase family
EWF20_RS11765	COG0517	IMP dehydrogenase activity
EWF20_RS11765	COG0516	GMP reductase activity
EWF20_RS11770	COG2513	Catalyzes the thermodynamically favored C-C bond cleavage of (2R,3S)-2-methylisocitrate to yield pyruvate and succinate
EWF20_RS11775	COG2079	MmgE PrpD family protein
EWF20_RS11780	COG0039	L-malate dehydrogenase activity
EWF20_RS11795	COG1968	undecaprenyl-diphosphatase activity
EWF20_RS11850	COG1446	asparaginase
EWF20_RS11865	COG0467	Core component of the KaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria. Binds to DNA. The KaiABC complex may act as a promoter-nonspecific transcription regulator that represses transcription, possibly by acting on the state of chromosome compaction
EWF20_RS11895	COG4231	catalyzes the ferredoxin-dependent oxidative decarboxylation of arylpyruvates
EWF20_RS11905	COG0437	4 iron, 4 sulfur cluster binding
EWF20_RS11910	COG0243	molybdopterin cofactor binding
EWF20_RS11940	COG3386	PFAM SMP-30 Gluconolaconase
EWF20_RS12060	COG0475	glutathione-regulated potassium exporter activity
EWF20_RS12080	COG0422	Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction
EWF20_RS12085	COG1249	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family
EWF20_RS12150	COG0425	Belongs to the sulfur carrier protein TusA family
EWF20_RS12155	COG2210	Belongs to the sulfur carrier protein TusA family
EWF20_RS12210	COG0095	Lipoate-protein ligase
EWF20_RS12235	COG0603	Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0))
EWF20_RS12245	COG0509	glycine decarboxylation via glycine cleavage system
EWF20_RS12250	COG0509	glycine decarboxylation via glycine cleavage system
EWF20_RS12255	COG0095	Lipoate-protein ligase
EWF20_RS12280	COG1914	H( )-stimulated, divalent metal cation uptake system
EWF20_RS12300	COG1702	phosphate starvation-inducible protein PhoH
EWF20_RS12305	COG0708	Exodeoxyribonuclease III
EWF20_RS12315	COG1444	tRNA N-acetyltransferase activity
EWF20_RS12355	COG0365	Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA
EWF20_RS12370	COG0491	Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid
EWF20_RS12375	COG0114	fumarate hydratase activity
EWF20_RS12405	COG0149	triose-phosphate isomerase activity
EWF20_RS12425	COG4148	Part of the ABC transporter complex ModABC involved in molybdenum import. Responsible for energy coupling to the transport system
EWF20_RS12430	COG2379	hydroxypyruvate reductase
EWF20_RS12440	COG1321	iron dependent repressor
EWF20_RS12460	COG2226	methyltransferase
EWF20_RS12475	COG0303	'Molybdopterin
EWF20_RS12480	COG0746	molybdenum cofactor guanylyltransferase activity
EWF20_RS12545	COG1062	S-(hydroxymethyl)glutathione dehydrogenase activity
EWF20_RS12550	COG0472	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan
EWF20_RS12555	COG0142	geranyltranstransferase activity
EWF20_RS12560	COG1304	FMN binding
EWF20_RS12570	COG1355	regulation of microtubule-based process
EWF20_RS12575	COG0052	Belongs to the universal ribosomal protein uS2 family
EWF20_RS12585	COG0103	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
EWF20_RS12590	COG0102	This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly
EWF20_RS12595	COG0200	Binds to the 23S rRNA
EWF20_RS12605	COG0100	Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome
EWF20_RS12610	COG0522	One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit
EWF20_RS12615	COG0099	Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits
EWF20_RS12625	COG0432	Pfam Uncharacterised protein family UPF0047
EWF20_RS12635	COG0162	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)
EWF20_RS12645	COG0417	DNA replication proofreading
EWF20_RS12680	COG1190	Belongs to the class-II aminoacyl-tRNA synthetase family
EWF20_RS12710	COG2055	Belongs to the LDH2 MDH2 oxidoreductase family
EWF20_RS12735	COG1250	3-hydroxyacyl-CoA dehydrogenase
EWF20_RS12740	COG1541	Catalyzes the activation of phenylacetic acid (PA) to phenylacetyl-CoA (PA-CoA)
EWF20_RS12775	COG2267	carboxylic ester hydrolase activity
EWF20_RS12785	COG1892	Catalyzes the irreversible beta-carboxylation of phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four- carbon dicarboxylic acid source for the tricarboxylic acid cycle
EWF20_RS12790	COG1225	Alkyl hydroperoxide reductase Thiol specific antioxidant Mal allergen
EWF20_RS12795	COG0062	Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration
EWF20_RS12795	COG0063	ADP-dependent NAD(P)H-hydrate dehydratase activity
EWF20_RS12820	COG0419	ATPase involved in DNA repair
EWF20_RS12820	COG0419	ATPase involved in DNA repair
EWF20_RS12895	COG3118	belongs to the thioredoxin family
EWF20_RS12955	COG0492	ferredoxin-NADP+ reductase activity
EWF20_RS12965	COG0061	Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP
EWF20_RS12980	COG0365	Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA
EWF20_RS12985	COG0520	Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L- selenocystine to produce L-alanine
EWF20_RS12995	COG2038	nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity
EWF20_RS13010	COG2265	Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family
EWF20_RS13015	COG0561	phosphatase activity
EWF20_RS13040	COG0494	nUDIX hydrolase
EWF20_RS13055	COG1618	nucleotide phosphatase activity, acting on free nucleotides
EWF20_RS13065	COG0171	NAD+ synthase (glutamine-hydrolyzing) activity
EWF20_RS13070	COG0388	Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source
EWF20_RS13070	COG0171	NAD+ synthase (glutamine-hydrolyzing) activity
EWF20_RS13095	COG2154	pterin-4-alpha-carbinolamine dehydratase
EWF20_RS13115	COG0538	isocitrate dehydrogenase activity
EWF20_RS13160	COG0500	methyltransferase
EWF20_RS13160	COG3615	Tellurite resistance protein tehB
EWF20_RS13185	COG2096	Adenosyltransferase
EWF20_RS13190	COG0697	spore germination
EWF20_RS13195	COG1207	Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP- GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5- monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain
EWF20_RS13205	COG0449	glutamine-fructose-6-phosphate transaminase (isomerizing) activity
EWF20_RS13210	COG0441	Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr)
EWF20_RS13215	COG1236	Exonuclease of the beta-lactamase fold involved in RNA processing
EWF20_RS13270	COG0069	glutamate synthase activity
EWF20_RS13275	COG0067	glutamate synthase
EWF20_RS13295	COG0119	Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)
EWF20_RS13335	COG1695	Transcriptional regulator
EWF20_RS13360	COG1351	Catalyzes the reductive methylation of 2'-deoxyuridine- 5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor, and NAD(P)H and FADH(2) as the reductant
EWF20_RS13370	COG0852	NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient
EWF20_RS13375	COG0649	NAD binding
EWF20_RS13380	COG1005	NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. This subunit may bind ubiquinone
EWF20_RS13385	COG1143	NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient
EWF20_RS13395	COG0713	ATP synthesis coupled electron transport
EWF20_RS13400	COG1009	NADH ubiquinone oxidoreductase subunit 5 chain L Multisubunit Na H antiporter, MnhA subunit
EWF20_RS13405	COG1007	ATP synthesis coupled electron transport
EWF20_RS13410	COG1260	myo-inositol-1-phosphate synthase
EWF20_RS13415	COG0607	Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS
EWF20_RS13415	COG0301	tRNA thio-modification
EWF20_RS13455	COG0329	Catalyzes the condensation of (S)-aspartate-beta- semialdehyde (S)-ASA and pyruvate to 4-hydroxy- tetrahydrodipicolinate (HTPA)
EWF20_RS13465	COG0730	response to heat
EWF20_RS13475	COG1573	uracil-dna glycosylase
EWF20_RS13480	COG1028	Short-chain dehydrogenase reductase Sdr
EWF20_RS13485	COG1195	it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP
EWF20_RS13495	COG0334	Belongs to the Glu Leu Phe Val dehydrogenases family
EWF20_RS13510	COG0215	cysteine-tRNA ligase activity
EWF20_RS13515	COG2222	sugar isomerase
EWF20_RS13535	COG0433	COG0433 Predicted ATPase
EWF20_RS13545	COG1607	acyl-coa hydrolase
EWF20_RS13550	COG1063	alcohol dehydrogenase
EWF20_RS13565	COG1055	Involved in arsenical resistance. Thought to form the channel of an arsenite pump
EWF20_RS13570	COG1960	acyl-CoA dehydrogenase activity
EWF20_RS13640	COG0476	Involved in molybdopterin and thiamine biosynthesis, family 2
EWF20_RS13645	COG3959	Transketolase, thiamine diphosphate binding domain
EWF20_RS13650	COG3958	Transketolase
EWF20_RS13655	COG0287	Prephenate dehydrogenase
EWF20_RS13655	COG1605	Chorismate mutase
EWF20_RS13660	COG2876	3-deoxy-7-phosphoheptulonate synthase activity
EWF20_RS13665	COG0337	Catalyzes the conversion of 3-deoxy-D-arabino- heptulosonate 7-phosphate (DAHP) to dehydroquinate (DHQ)
EWF20_RS13670	COG0169	Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA)
EWF20_RS13675	COG0082	Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system
EWF20_RS13680	COG0083	homoserine kinase activity
EWF20_RS13685	COG0128	3-phosphoshikimate 1-carboxyvinyltransferase activity
EWF20_RS13705	COG1199	ATP-dependent helicase activity
EWF20_RS13725	COG0605	Destroys radicals which are normally produced within the cells and which are toxic to biological systems
EWF20_RS13730	COG1208	COG1208 Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis translation initiation factor 2B, gamma epsilon subunits eIF-2Bgamma eIF-2Bepsilon
EWF20_RS13740	COG1331	Highly conserved protein containing a thioredoxin domain
EWF20_RS13745	COG1028	Short-chain dehydrogenase reductase Sdr
EWF20_RS13760	COG1637	Cleaves both 3' and 5' ssDNA extremities of branched DNA structures
EWF20_RS13780	COG1192	Involved in chromosome partitioning
EWF20_RS13790	COG0277	FAD linked oxidase domain protein
EWF20_RS13805	COG1013	pyruvate-flavodoxin oxidoreductase activity
EWF20_RS13810	COG0674	pyruvate-flavodoxin oxidoreductase activity
EWF20_RS13810	COG1014	Oxidoreductase required for the transfer of electrons from pyruvate to flavodoxin
EWF20_RS13830	COG0618	3'(2'),5'-bisphosphate nucleotidase activity
EWF20_RS13830	COG0517	IMP dehydrogenase activity
EWF20_RS13830	COG0617	tRNA 3'-terminal CCA addition
EWF20_RS13835	COG0123	including yeast histone deacetylase and acetoin utilization protein
EWF20_RS13840	COG0027	Involved in the de novo purine biosynthesis. Catalyzes the transfer of formate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR). Formate is provided by PurU via hydrolysis of 10-formyl-tetrahydrofolate
EWF20_RS13885	COG1201	RNA secondary structure unwinding
EWF20_RS13925	COG0006	proline dipeptidase activity
EWF20_RS13930	COG0123	including yeast histone deacetylase and acetoin utilization protein
EWF20_RS13945	COG1234	tRNA 3'-trailer cleavage
EWF20_RS13950	COG0524	Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5- phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway
EWF20_RS13955	COG0351	phosphomethylpyrimidine kinase
EWF20_RS13965	COG1215	transferase activity, transferring glycosyl groups
EWF20_RS13985	COG0463	Glycosyl transferase, family 2
EWF20_RS14005	COG1063	alcohol dehydrogenase
EWF20_RS14010	COG1270	Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group
EWF20_RS14025	COG0518	GMP synthase (glutamine-hydrolyzing) activity
EWF20_RS14025	COG0519	Catalyzes the synthesis of GMP from XMP
EWF20_RS14030	COG0501	Belongs to the peptidase M48B family
EWF20_RS14045	COG1830	Aldolase
EWF20_RS14060	COG1087	Belongs to the NAD(P)-dependent epimerase dehydratase family
EWF20_RS14070	COG0405	Gamma-glutamyltransferase
EWF20_RS14085	COG1444	tRNA N-acetyltransferase activity
EWF20_RS14095	COG0233	cytoplasmic translational termination
EWF20_RS14110	COG0589	response to stress
EWF20_RS14115	COG2271	Major facilitator Superfamily
EWF20_RS14135	COG0639	Hydrolyzes diadenosine 5',5'''-P1,P4-tetraphosphate to yield ADP
EWF20_RS14155	COG0516	GMP reductase activity
EWF20_RS14155	COG0517	IMP dehydrogenase activity
EWF20_RS14170	COG0730	response to heat
EWF20_RS14205	COG2110	phosphatase homologous to the C-terminal domain of histone macroH2A1
EWF20_RS14205	COG2256	atpase related to the helicase subunit of the holliday junction resolvase
EWF20_RS14220	COG0438	transferase activity, transferring glycosyl groups
EWF20_RS14230	COG1846	Transcriptional regulator
EWF20_RS14280	COG0843	Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1- 3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B
EWF20_RS14305	COG0621	Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine
EWF20_RS14310	COG1520	Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane
EWF20_RS14320	COG0402	S-adenosylhomocysteine deaminase activity
EWF20_RS14330	COG1573	uracil-dna glycosylase
EWF20_RS14355	COG0028	Belongs to the TPP enzyme family
EWF20_RS14365	COG0535	radical SAM domain protein
EWF20_RS14370	COG1250	3-hydroxyacyl-CoA dehydrogenase
EWF20_RS14390	COG1024	Enoyl-CoA hydratase
EWF20_RS14395	COG0183	Belongs to the thiolase family
EWF20_RS14420	COG2814	Major facilitator Superfamily
EWF20_RS14450	COG2271	Major facilitator Superfamily
EWF20_RS14460	COG1013	pyruvate-flavodoxin oxidoreductase activity
EWF20_RS14465	COG1014	Oxidoreductase required for the transfer of electrons from pyruvate to flavodoxin
EWF20_RS14465	COG0674	pyruvate-flavodoxin oxidoreductase activity
EWF20_RS14485	COG0515	serine threonine protein kinase
EWF20_RS14485	COG2815	serine threonine protein kinase
EWF20_RS14495	COG2235	arginine
EWF20_RS14500	COG0450	alkyl hydroperoxide reductase
EWF20_RS14505	COG3376	Belongs to the NiCoT transporter (TC 2.A.52) family
EWF20_RS14525	COG1720	tRNA m6t6A37 methyltransferase activity
EWF20_RS14555	COG2271	Major facilitator Superfamily
EWF20_RS14615	COG0530	calcium, potassium:sodium antiporter activity
EWF20_RS14620	COG2149	membrane
EWF20_RS14625	COG3276	selenocysteine insertion sequence binding
EWF20_RS14630	COG1520	Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane
EWF20_RS14645	COG3842	ATPase activity
EWF20_RS14660	COG0531	amino acid
EWF20_RS14665	COG1012	belongs to the aldehyde dehydrogenase family
EWF20_RS14670	COG0524	Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5- phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway
EWF20_RS14675	COG0329	Catalyzes the condensation of (S)-aspartate-beta- semialdehyde (S)-ASA and pyruvate to 4-hydroxy- tetrahydrodipicolinate (HTPA)
EWF20_RS14680	COG0697	spore germination
EWF20_RS14695	COG1252	NADH dehydrogenase
EWF20_RS14710	COG0586	Pfam SNARE associated Golgi protein
EWF20_RS14730	COG1335	isochorismatase
EWF20_RS14735	COG1131	(ABC) transporter
EWF20_RS14750	COG0412	Dienelactone hydrolase