locus-tag	class	description
TWT_RS00005	COG0593	it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box) 5'-TTATC CA A CA A-3'. DnaA binds to ATP and to acidic phospholipids
TWT_RS00010	COG0592	DNA-directed DNA polymerase activity
TWT_RS00015	COG1195	it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP
TWT_RS00025	COG0187	DNA topoisomerase II activity
TWT_RS00030	COG0188	A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner
TWT_RS00050	COG1564	THIamine pyrophosphokinase
TWT_RS00070	COG1704	LemA family
TWT_RS00075	COG0501	Belongs to the peptidase M48B family
TWT_RS00095	COG1109	Catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate
TWT_RS00110	COG4770	biotin carboxylase activity
TWT_RS00130	COG4799	Acetyl-CoA carboxylase, carboxyltransferase component subunits alpha and beta
TWT_RS00170	COG1898	dTDP-4-dehydrorhamnose 3,5-epimerase activity
TWT_RS00205	COG1216	Glycosyl transferase, family 2
TWT_RS00250	COG0745	RESPONSE REGULATOR receiver
TWT_RS00255	COG1278	Cold shock
TWT_RS00270	COG0287	Prephenate dehydrogenase
TWT_RS00275	COG1160	GTP binding
TWT_RS00280	COG1169	isochorismate synthase
TWT_RS00285	COG2226	methyltransferase
TWT_RS00290	COG0142	geranyltranstransferase activity
TWT_RS00310	COG1120	ATPase activity
TWT_RS00315	COG4779	Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily
TWT_RS00320	COG0609	Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily
TWT_RS00335	COG0640	Transcriptional regulator
TWT_RS00345	COG1384	Belongs to the class-I aminoacyl-tRNA synthetase family
TWT_RS00350	COG0414	Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate
TWT_RS00360	COG1502	Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol
TWT_RS00380	COG1165	Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate (SEPHCHC)
TWT_RS00395	COG0085	RNA polymerase activity
TWT_RS00400	COG0086	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates
TWT_RS00410	COG0234	Binds to Cpn60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter
TWT_RS00420	COG0516	GMP reductase activity
TWT_RS00435	COG0519	Catalyzes the synthesis of GMP from XMP
TWT_RS00435	COG0518	GMP synthase (glutamine-hydrolyzing) activity
TWT_RS00455	COG0299	Catalyzes the transfer of a formyl group from 10- formyltetrahydrofolate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR) and tetrahydrofolate
TWT_RS00460	COG0138	bifunctional purine biosynthesis protein purh
TWT_RS00480	COG0750	zinc metalloprotease
TWT_RS00485	COG0743	1-deoxy-D-xylulose-5-phosphate reductoisomerase activity
TWT_RS00490	COG0631	protein serine/threonine phosphatase activity
TWT_RS00510	COG1131	(ABC) transporter
TWT_RS00540	COG4974	Belongs to the 'phage' integrase family
TWT_RS00565	COG0504	CTP synthase activity
TWT_RS00595	COG0497	DNA recombination
TWT_RS00600	COG0061	Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP
TWT_RS00610	COG0359	binds to the 23S rRNA
TWT_RS00615	COG0238	Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit
TWT_RS00620	COG0629	single-stranded DNA binding
TWT_RS00625	COG0360	Binds together with S18 to 16S ribosomal RNA
TWT_RS00635	COG0152	phosphoribosylaminoimidazolesuccinocarboxamide synthase activity
TWT_RS00640	COG0447	Converts o-succinylbenzoyl-CoA (OSB-CoA) to 1,4- dihydroxy-2-naphthoyl-CoA (DHNA-CoA)
TWT_RS00660	COG0227	Belongs to the bacterial ribosomal protein bL28 family
TWT_RS00665	COG0267	Belongs to the bacterial ribosomal protein bL33 family
TWT_RS00670	COG0199	Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site
TWT_RS00675	COG0776	regulation of translation
TWT_RS00685	COG0553	Transcription regulator that activates transcription by stimulating RNA polymerase (RNAP) recycling in case of stress conditions such as supercoiled DNA or high salt concentrations. Probably acts by releasing the RNAP, when it is trapped or immobilized on tightly supercoiled DNA. Does not activate transcription on linear DNA. Probably not involved in DNA repair
TWT_RS00690	COG0050	This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis
TWT_RS00695	COG1928	C-terminal four TMM region of protein-O-mannosyltransferase
TWT_RS00700	COG1482	cell wall glycoprotein biosynthetic process
TWT_RS00725	COG1109	Catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate
TWT_RS00735	COG0653	protein targeting
TWT_RS00760	COG1003	The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor
TWT_RS00760	COG0403	The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor
TWT_RS00780	COG1122	ATPase activity
TWT_RS00785	COG0601	transmembrane transport
TWT_RS00790	COG1173	ABC-type dipeptide oligopeptide nickel transport systems, permease components
TWT_RS00830	COG0102	This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly
TWT_RS00835	COG0103	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
TWT_RS00840	COG1109	Catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate
TWT_RS00890	COG0343	queuine tRNA-ribosyltransferase activity
TWT_RS00900	COG0221	Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) forming two phosphate ions
TWT_RS00915	COG0532	One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex
TWT_RS00940	COG0620	Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation
TWT_RS00945	COG0290	IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins
TWT_RS00950	COG0291	Belongs to the bacterial ribosomal protein bL35 family
TWT_RS00955	COG0292	Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit
TWT_RS00960	COG0016	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily
TWT_RS00970	COG0162	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)
TWT_RS01030	COG1131	(ABC) transporter
TWT_RS01040	COG0534	Mate efflux family protein
TWT_RS01055	COG0442	prolyl-tRNA aminoacylation
TWT_RS01070	COG0821	Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
TWT_RS01075	COG0462	Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib-5-P)
TWT_RS01080	COG1207	Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP- GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5- monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain
TWT_RS01170	COG0540	Belongs to the ATCase OTCase family
TWT_RS01185	COG1186	Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA
TWT_RS01190	COG2884	Cell division ATP-binding protein ftsE
TWT_RS01195	COG2177	Part of the ABC transporter FtsEX involved in
TWT_RS01200	COG0691	Required for rescue of stalled ribosomes mediated by trans-translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene
TWT_RS01225	COG0030	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity
TWT_RS01275	COG0129	dihydroxy-acid dehydratase activity
TWT_RS01280	COG0028	Belongs to the TPP enzyme family
TWT_RS01285	COG0440	acetolactate synthase activity
TWT_RS01290	COG0059	Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol- acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3- dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3- hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate
TWT_RS01295	COG0115	Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family
TWT_RS01300	COG0008	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)
TWT_RS01305	COG0492	ferredoxin-NADP+ reductase activity
TWT_RS01305	COG0492	ferredoxin-NADP+ reductase activity
TWT_RS01315	COG1268	BioY protein
TWT_RS01325	COG1940	carbohydrate kinase activity
TWT_RS01335	COG3200	phospho-2-dehydro-3-deoxyheptonate aldolase
TWT_RS01345	COG0515	serine threonine protein kinase
TWT_RS01345	COG2815	serine threonine protein kinase
TWT_RS01350	COG0142	geranyltranstransferase activity
TWT_RS01365	COG2001	Belongs to the MraZ family
TWT_RS01370	COG0275	rRNA processing
TWT_RS01385	COG0770	UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity
TWT_RS01390	COG0472	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan
TWT_RS01395	COG0769	Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan
TWT_RS01400	COG0771	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)
TWT_RS01405	COG0772	Belongs to the SEDS family
TWT_RS01410	COG0707	Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc- (pentapeptide)GlcNAc (lipid intermediate II)
TWT_RS01415	COG0773	UDP-N-acetylmuramate-L-alanine ligase activity
TWT_RS01455	COG1327	Negatively regulates transcription of bacterial ribonucleotide reductase nrd genes and operons by binding to NrdR- boxes
TWT_RS01460	COG0167	dihydroorotate dehydrogenase activity
TWT_RS01465	COG0328	RNA-DNA hybrid ribonuclease activity
TWT_RS01485	COG1622	oxidoreductase activity, acting on a heme group of donors, oxygen as acceptor
TWT_RS01490	COG0843	Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1- 3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B
TWT_RS01500	COG1845	cytochrome c oxidase, subunit III
TWT_RS01505	COG2010	Cytochrome c
TWT_RS01510	COG0723	plastoquinol--plastocyanin reductase activity
TWT_RS01515	COG1290	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis
TWT_RS01525	COG0304	beta-ketoacyl-acyl-carrier-protein synthase II activity
TWT_RS01530	COG0236	Carrier of the growing fatty acid chain in fatty acid biosynthesis
TWT_RS01535	COG0332	Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched- chain and or straight-chain of fatty acids
TWT_RS01545	COG2609	component of the pyruvate dehydrogenase (PDH) complex, that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2)
TWT_RS01560	COG0327	Belongs to the GTP cyclohydrolase I type 2 NIF3 family
TWT_RS01575	COG0413	3-methyl-2-oxobutanoate hydroxymethyltransferase activity
TWT_RS01585	COG0441	Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr)
TWT_RS01595	COG0214	Catalyzes the formation of pyridoxal 5'-phosphate from ribose 5-phosphate (RBP), glyceraldehyde 3-phosphate (G3P) and ammonia. The ammonia is provided by the PdxT subunit. Can also use ribulose 5-phosphate and dihydroxyacetone phosphate as substrates, resulting from enzyme-catalyzed isomerization of RBP and G3P, respectively
TWT_RS01600	COG0311	Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS
TWT_RS01605	COG0217	transcriptional regulatory protein
TWT_RS01610	COG0817	Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group
TWT_RS01615	COG0632	four-way junction helicase activity
TWT_RS01630	COG0342	Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA
TWT_RS01635	COG0341	Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA
TWT_RS01645	COG0317	In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance
TWT_RS01650	COG0268	Binds directly to 16S ribosomal RNA
TWT_RS01655	COG0481	GTPase activity
TWT_RS01665	COG0484	heat shock protein binding
TWT_RS01670	COG1385	rRNA (uridine-N3-)-methyltransferase activity
TWT_RS01675	COG0537	adenosine 5'-monophosphoramidase activity
TWT_RS01680	COG0319	Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA
TWT_RS01685	COG1159	An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism
TWT_RS01690	COG1381	Involved in DNA repair and RecF pathway recombination
TWT_RS01695	COG0020	Catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic pyrophosphates generating different type of terpenoids
TWT_RS01700	COG0232	Belongs to the dGTPase family. Type 2 subfamily
TWT_RS01715	COG0791	NLP P60 protein
TWT_RS01725	COG0469	pyruvate kinase activity
TWT_RS01740	COG0258	In addition to polymerase activity, this DNA polymerase exhibits 5'-3' exonuclease activity
TWT_RS01740	COG0749	In addition to polymerase activity, this DNA polymerase exhibits 5'-3' exonuclease activity
TWT_RS01750	COG0237	dephospho-CoA kinase activity
TWT_RS01765	COG0322	excinuclease ABC activity
TWT_RS01780	COG0057	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
TWT_RS01785	COG0126	phosphoglycerate kinase activity
TWT_RS01790	COG0149	triose-phosphate isomerase activity
TWT_RS01810	COG0221	Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) forming two phosphate ions
TWT_RS01815	COG0363	glucosamine-6-phosphate deaminase activity
TWT_RS01820	COG0682	Transfers the N-acyl diglyceride group on what will become the N-terminal cysteine of membrane lipoproteins
TWT_RS01825	COG0242	peptide deformylase activity
TWT_RS01835	COG0438	transferase activity, transferring glycosyl groups
TWT_RS01840	COG1028	Short-chain dehydrogenase reductase Sdr
TWT_RS01845	COG0254	rRNA binding
TWT_RS01855	COG1968	undecaprenyl-diphosphatase activity
TWT_RS01860	COG0215	cysteine-tRNA ligase activity
TWT_RS01885	COG0805	protein transport
TWT_RS01900	COG0084	hydrolase, TatD family'
TWT_RS01905	COG1063	alcohol dehydrogenase
TWT_RS01915	COG4213	ABC transporter substrate-binding protein
TWT_RS01920	COG4214	carbohydrate transport
TWT_RS01930	COG0020	Catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic pyrophosphates generating different type of terpenoids
TWT_RS01950	COG2151	metal-sulfur cluster biosynthetic enzyme
TWT_RS01955	COG0396	ATPase activity
TWT_RS01970	COG0719	Fe-S assembly protein
TWT_RS01975	COG1612	heme a metabolic process
TWT_RS01985	COG0176	Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway
TWT_RS01990	COG0166	Belongs to the GPI family
TWT_RS02020	COG0566	Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family
TWT_RS02025	COG0215	cysteine-tRNA ligase activity
TWT_RS02030	COG1211	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity
TWT_RS02030	COG0245	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity
TWT_RS02035	COG1329	Transcriptional regulator
TWT_RS02045	COG0018	arginyl-trna synthetase
TWT_RS02055	COG1117	ATPase-coupled phosphate ion transmembrane transporter activity
TWT_RS02065	COG0573	inorganic phosphate transmembrane transporter activity
TWT_RS02070	COG0226	Part of the ABC transporter complex PstSACB involved in phosphate import
TWT_RS02085	COG0223	Attaches a formyl group to the free amino group of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus
TWT_RS02095	COG0192	Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme
TWT_RS02105	COG1758	RNA polymerase activity
TWT_RS02115	COG0284	orotidine-5'-phosphate decarboxylase activity
TWT_RS02120	COG0458	carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
TWT_RS02125	COG0505	Belongs to the CarA family
TWT_RS02130	COG0044	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides
TWT_RS02135	COG0231	Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase
TWT_RS02140	COG0757	Catalyzes a trans-dehydration via an enolate intermediate
TWT_RS02145	COG0337	Catalyzes the conversion of 3-deoxy-D-arabino- heptulosonate 7-phosphate (DAHP) to dehydroquinate (DHQ)
TWT_RS02150	COG0703	Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate
TWT_RS02155	COG0082	Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system
TWT_RS02160	COG0169	Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA)
TWT_RS02170	COG0816	Could be a nuclease involved in processing of the 5'-end of pre-16S rRNA
TWT_RS02175	COG0013	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain
TWT_RS02185	COG0522	One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit
TWT_RS02215	COG1555	photosystem II stabilization
TWT_RS02215	COG1596	polysaccharide export
TWT_RS02220	COG0495	leucyl-tRNA aminoacylation
TWT_RS02225	COG0147	Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine- binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia
TWT_RS02285	COG0349	Exonuclease involved in the 3' processing of various precursor tRNAs. Initiates hydrolysis at the 3'-terminus of an RNA molecule and releases 5'-mononucleotides
TWT_RS02305	COG2761	dithiol-disulfide isomerase involved in polyketide biosynthesis
TWT_RS02330	COG0629	single-stranded DNA binding
TWT_RS02340	COG0171	NAD+ synthase (glutamine-hydrolyzing) activity
TWT_RS02350	COG1949	3'-to-5' exoribonuclease specific for small oligoribonucleotides
TWT_RS02370	COG3845	ABC transporter
TWT_RS02385	COG0064	Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln)
TWT_RS02390	COG0154	amidase activity
TWT_RS02395	COG0721	Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln)
TWT_RS02400	COG0272	DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA
TWT_RS02405	COG0482	sulfurtransferase activity
TWT_RS02410	COG1104	cysteine desulfurase activity
TWT_RS02435	COG1185	polyribonucleotide nucleotidyltransferase activity
TWT_RS02440	COG0184	Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome
TWT_RS02445	COG0355	proton-transporting ATP synthase activity, rotational mechanism
TWT_RS02450	COG0055	Produces ATP from ADP in the presence of a proton gradient across the membrane. The catalytic sites are hosted primarily by the beta subunits
TWT_RS02455	COG0224	Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex
TWT_RS02460	COG0056	Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit
TWT_RS02465	COG0712	F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation
TWT_RS02470	COG0711	Component of the F(0) channel, it forms part of the peripheral stalk, linking F(1) to F(0)
TWT_RS02475	COG0636	F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation
TWT_RS02480	COG0356	it plays a direct role in the translocation of protons across the membrane
TWT_RS02490	COG0472	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan
TWT_RS02500	COG2890	protein-(glutamine-N5) methyltransferase activity
TWT_RS02505	COG0216	Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA
TWT_RS02515	COG0083	homoserine kinase activity
TWT_RS02520	COG0498	Catalyzes the gamma-elimination of phosphate from L- phosphohomoserine and the beta-addition of water to produce L- threonine
TWT_RS02525	COG0460	homoserine dehydrogenase
TWT_RS02555	COG0575	Belongs to the CDS family
TWT_RS02560	COG0233	cytoplasmic translational termination
TWT_RS02565	COG0528	UMP kinase activity
TWT_RS02570	COG0264	Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome
TWT_RS02575	COG0052	Belongs to the universal ribosomal protein uS2 family
TWT_RS02590	COG4974	Belongs to the 'phage' integrase family
TWT_RS02595	COG0758	DNA mediated transformation
TWT_RS02605	COG0792	nuclease activity
TWT_RS02615	COG0335	large ribosomal subunit rRNA binding
TWT_RS02620	COG0336	Belongs to the RNA methyltransferase TrmD family
TWT_RS02625	COG0806	An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes
TWT_RS02630	COG1837	Belongs to the UPF0109 family
TWT_RS02635	COG0228	four-way junction DNA binding
TWT_RS02640	COG0541	Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY
TWT_RS02645	COG0321	lipoyl(octanoyl) transferase activity
TWT_RS02650	COG0320	Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives
TWT_RS02680	COG1057	Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD)
TWT_RS02690	COG0536	An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control
TWT_RS02695	COG0211	Belongs to the bacterial ribosomal protein bL27 family
TWT_RS02700	COG0261	This protein binds to 23S rRNA in the presence of protein L20
TWT_RS02705	COG1530	ribonuclease E activity
TWT_RS02710	COG0105	UTP biosynthetic process
TWT_RS02720	COG0285	dihydrofolate synthase activity
TWT_RS02725	COG0060	amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile)
TWT_RS02730	COG0525	valine-tRNA ligase activity
TWT_RS02735	COG1219	ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of ClpP
TWT_RS02740	COG0740	serine-type endopeptidase activity
TWT_RS02745	COG0740	serine-type endopeptidase activity
TWT_RS02755	COG0544	peptidyl-prolyl cis-trans isomerase activity
TWT_RS02770	COG1154	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP)
TWT_RS02785	COG0756	dUTP diphosphatase activity
TWT_RS02805	COG0188	A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner
TWT_RS02810	COG0362	Catalyzes the oxidative decarboxylation of 6- phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH
TWT_RS02825	COG0187	DNA topoisomerase II activity
TWT_RS02830	COG0787	Catalyzes the interconversion of L-alanine and D- alanine. May also act on other amino acids
TWT_RS02835	COG0787	Catalyzes the interconversion of L-alanine and D- alanine. May also act on other amino acids
TWT_RS02850	COG0568	sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released
TWT_RS02855	COG0752	glycyl-tRNA synthetase alpha subunit
TWT_RS02855	COG0751	glycine-tRNA ligase activity
TWT_RS02860	COG0260	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides
TWT_RS02865	COG1249	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family
TWT_RS02870	COG0508	dehydrogenase complex catalyzes the overall conversion of
TWT_RS02880	COG1181	Belongs to the D-alanine--D-alanine ligase family
TWT_RS02885	COG0240	glycerol-3-phosphate dehydrogenase [NAD(P)+] activity
TWT_RS02890	COG0204	Acyl-transferase
TWT_RS02895	COG0766	Cell wall formation. Adds enolpyruvyl to UDP-N- acetylglucosamine
TWT_RS02900	COG0861	Integral membrane protein TerC family
TWT_RS02910	COG0587	DNA-directed DNA polymerase activity
TWT_RS02920	COG0597	This protein specifically catalyzes the removal of signal peptides from prolipoproteins
TWT_RS02925	COG3599	regulation of cell shape
TWT_RS02930	COG1799	cell septum assembly
TWT_RS02935	COG0206	Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity
TWT_RS02955	COG0210	DNA helicase
TWT_RS02975	COG0006	proline dipeptidase activity
TWT_RS02980	COG2239	Acts as a magnesium transporter
TWT_RS02995	COG0101	tRNA pseudouridine synthase activity
TWT_RS03000	COG0203	ribosomal protein L17
TWT_RS03005	COG0202	RNA polymerase activity
TWT_RS03010	COG0100	Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome
TWT_RS03015	COG0099	Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits
TWT_RS03020	COG0361	One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre- initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initation complex
TWT_RS03030	COG0563	adenylate kinase activity
TWT_RS03040	COG0200	Binds to the 23S rRNA
TWT_RS03045	COG1841	ribosomal large subunit assembly
TWT_RS03050	COG0098	Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body
TWT_RS03055	COG0256	This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance
TWT_RS03060	COG0097	This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7 L12 stalk, and near the tRNA binding site of the peptidyltransferase center
TWT_RS03065	COG0096	One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit
TWT_RS03070	COG0094	This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits
TWT_RS03075	COG0198	One of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit
TWT_RS03080	COG0093	Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome
TWT_RS03085	COG0186	One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA
TWT_RS03090	COG0255	Belongs to the universal ribosomal protein uL29 family
TWT_RS03095	COG0197	Binds 23S rRNA and is also seen to make contacts with the A and possibly P site tRNAs
TWT_RS03100	COG0092	Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation
TWT_RS03105	COG0091	The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome
TWT_RS03110	COG0185	Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA
TWT_RS03115	COG0090	One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity
TWT_RS03120	COG0089	rRNA binding
TWT_RS03125	COG0088	Forms part of the polypeptide exit tunnel
TWT_RS03130	COG0087	One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit
TWT_RS03135	COG0051	transcription antitermination factor activity, RNA binding
TWT_RS03170	COG0571	ribonuclease III activity
TWT_RS03175	COG0333	Belongs to the bacterial ribosomal protein bL32 family
TWT_RS03185	COG0669	Reversibly transfers an adenylyl group from ATP to 4'- phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate
TWT_RS03215	COG4122	O-methyltransferase activity
TWT_RS03260	COG0196	riboflavin kinase activity
TWT_RS03265	COG0692	Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine
TWT_RS03275	COG1137	ATPase activity
TWT_RS03285	COG0294	Catalyzes the condensation of para-aminobenzoate (pABA) with 6-hydroxymethyl-7,8-dihydropterin diphosphate (DHPt-PP) to form 7,8-dihydropteroate (H2Pte), the immediate precursor of folate derivatives
TWT_RS03290	COG0302	gtp cyclohydrolase
TWT_RS03295	COG0465	Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins
TWT_RS03300	COG0634	Belongs to the purine pyrimidine phosphoribosyltransferase family
TWT_RS03305	COG0037	tRNA processing
TWT_RS03310	COG0533	Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction
TWT_RS03315	COG1214	PFAM Peptidase M22, glycoprotease
TWT_RS03315	COG0456	acetyltransferase
TWT_RS03320	COG0802	atpase or kinase
TWT_RS03325	COG1217	gtp-binding protein TYPA
TWT_RS03355	COG1132	(ABC) transporter
TWT_RS03360	COG1132	(ABC) transporter
TWT_RS03415	COG1947	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol
TWT_RS03435	COG0449	glutamine-fructose-6-phosphate transaminase (isomerizing) activity
TWT_RS03460	COG0324	Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)
TWT_RS03465	COG0621	Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine
TWT_RS03470	COG0468	Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage
TWT_RS03485	COG1674	ftsk spoiiie
TWT_RS03490	COG0595	An RNase that has 5'-3' exonuclease and possibly endonuclease activity. Involved in maturation of rRNA and in some organisms also mRNA maturation and or decay
TWT_RS03525	COG0127	Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA RNA and avoiding chromosomal lesions
TWT_RS03530	COG0689	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates
TWT_RS03570	COG0265	Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain
TWT_RS03595	COG1038	Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second
TWT_RS03600	COG1192	Involved in chromosome partitioning
TWT_RS03620	COG0190	Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate
TWT_RS03625	COG0112	Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF- independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism
TWT_RS03630	COG0742	ribosomal rna small subunit methyltransferase
TWT_RS03635	COG0509	glycine decarboxylation via glycine cleavage system
TWT_RS03640	COG0404	The glycine cleavage system catalyzes the degradation of glycine
TWT_RS03645	COG0012	GTP binding
TWT_RS03660	COG0761	Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)- butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP MEP pathway for isoprenoid precursor biosynthesis
TWT_RS03665	COG1570	exodeoxyribonuclease VII activity
TWT_RS03670	COG1722	exodeoxyribonuclease VII activity
TWT_RS03675	COG2271	Major facilitator Superfamily
TWT_RS03705	COG0193	aminoacyl-tRNA hydrolase activity
TWT_RS03720	COG1825	This is one of the proteins that binds to the 5S RNA in the ribosome where it forms part of the central protuberance
TWT_RS03725	COG0588	phosphoglycerate mutase activity
TWT_RS03755	COG1970	mechanosensitive ion channel activity
TWT_RS03765	COG1121	(ABC) transporter
TWT_RS03770	COG1108	ABC-type Mn2 Zn2 transport systems permease components
TWT_RS03810	COG0480	Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome
TWT_RS03815	COG0049	tRNA binding
TWT_RS03820	COG0048	Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit
TWT_RS03835	COG0782	Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus
TWT_RS03840	COG1171	Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short- lived. The second step is the nonenzymatic hydrolysis of the enamine imine intermediates to form 2-ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA
TWT_RS03845	COG0208	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides
TWT_RS03850	COG1327	Negatively regulates transcription of bacterial ribonucleotide reductase nrd genes and operons by binding to NrdR- boxes
TWT_RS03850	COG0209	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides
TWT_RS03865	COG0180	Tryptophanyl-tRNA synthetase
TWT_RS03875	COG0307	riboflavin synthase, alpha
TWT_RS03880	COG0807	Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate
TWT_RS03880	COG0108	Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate
TWT_RS03890	COG0054	6,7-dimethyl-8-ribityllumazine synthase activity
TWT_RS03895	COG1335	isochorismatase
TWT_RS03910	COG2812	DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity
TWT_RS03915	COG0125	Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis
TWT_RS03920	COG1754	DNA topoisomerase type I activity
TWT_RS03920	COG0550	Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone
TWT_RS03950	COG0251	oxidation-reduction process
TWT_RS03955	COG0744	Peptidoglycan polymerase that catalyzes glycan chain elongation from lipid-linked precursors
TWT_RS03955	COG4499	WXG100 protein secretion system (Wss), protein YukC
TWT_RS03975	COG0527	aspartate kinase activity
TWT_RS03980	COG0353	May play a role in DNA repair. It seems to be involved in an RecBC-independent recombinational process of DNA repair. It may act with RecF and RecO
TWT_RS03990	COG0222	ribosome binding
TWT_RS03995	COG0244	Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors
TWT_RS04000	COG0081	regulation of translation
TWT_RS04005	COG0080	Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors
TWT_RS04015	COG0690	P-P-bond-hydrolysis-driven protein transmembrane transporter activity
TWT_RS04025	COG0436	Aminotransferase
TWT_RS04030	COG0812	UDP-N-acetylmuramate dehydrogenase activity
TWT_RS04040	COG2030	dehydratase
TWT_RS04050	COG0150	phosphoribosylformylglycinamidine cyclo-ligase activity
TWT_RS04060	COG0151	Belongs to the GarS family
TWT_RS04065	COG0172	Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec)
TWT_RS04110	COG0181	hydroxymethylbilane synthase activity
TWT_RS04115	COG0276	Catalyzes the ferrous insertion into protoporphyrin IX
TWT_RS04120	COG3253	May function as heme-dependent peroxidase
TWT_RS04125	COG0407	Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III
TWT_RS04130	COG0373	Catalyzes the NADPH-dependent reduction of glutamyl- tRNA(Glu) to glutamate 1-semialdehyde (GSA)
TWT_RS04135	COG4948	Mandelate Racemase Muconate Lactonizing
TWT_RS04140	COG0305	Participates in initiation and elongation during chromosome replication
TWT_RS04165	COG0461	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)
TWT_RS04170	COG0708	Exodeoxyribonuclease III
TWT_RS04180	COG0542	response to heat
TWT_RS04185	COG0789	Transcriptional regulator
TWT_RS04195	COG0576	Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ
TWT_RS04200	COG0443	Heat shock 70 kDa protein
TWT_RS04205	COG0590	tRNA wobble adenosine to inosine editing
TWT_RS04220	COG0017	Asparaginyl-tRNA synthetase
TWT_RS04225	COG0406	alpha-ribazole phosphatase activity
TWT_RS04230	COG3118	belongs to the thioredoxin family
TWT_RS04235	COG0785	Cytochrome C biogenesis protein
TWT_RS04240	COG1333	cytochrome c biogenesis protein
TWT_RS04245	COG0755	Required for the export of heme to the periplasm for the biogenesis of c-type cytochromes
TWT_RS04255	COG1108	ABC-type Mn2 Zn2 transport systems permease components
TWT_RS04265	COG0143	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation
TWT_RS04275	COG0463	Glycosyl transferase, family 2
TWT_RS04295	COG3590	peptidase
TWT_RS04300	COG0546	phosphoglycolate phosphatase activity
TWT_RS04335	COG0768	penicillin binding
TWT_RS04340	COG0515	serine threonine protein kinase
TWT_RS04345	COG0515	serine threonine protein kinase
TWT_RS04345	COG2815	serine threonine protein kinase
TWT_RS04370	COG0148	phosphopyruvate hydratase activity
TWT_RS04380	COG0124	histidyl-tRNA synthetase
TWT_RS04385	COG3004	Na( ) H( ) antiporter that extrudes sodium in exchange for external protons
TWT_RS04390	COG0717	Belongs to the dCTP deaminase family
TWT_RS04400	COG0508	dehydrogenase complex catalyzes the overall conversion of
TWT_RS04405	COG0022	dehydrogenase e1 component
TWT_RS04420	COG0104	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP
TWT_RS04430	COG0046	Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL
TWT_RS04435	COG0047	phosphoribosylformylglycinamidine synthase activity
TWT_RS04465	COG1475	DNA binding
TWT_RS04470	COG1192	Involved in chromosome partitioning
TWT_RS04475	COG2217	Heavy metal translocating P-type atpase
TWT_RS04485	COG0706	membrane insertase activity
TWT_RS04490	COG0759	Could be involved in insertion of integral membrane proteins into the membrane
TWT_RS04495	COG0594	ribonuclease P activity
TWT_RS04515	COG0858	One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Required for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA
TWT_RS04520	COG0073	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation
TWT_RS04520	COG0072	phenylalanine-tRNA ligase activity
TWT_RS04530	COG0194	GDP metabolic process