LOCUS NC_038672 11391 bp RNA linear VRL 24-AUG-2018 DEFINITION Mucambo virus strain Mucambo BeAn 8, complete genome. ACCESSION NC_038672 VERSION NC_038672.1 DBLINK BioProject: PRJNA485481 KEYWORDS RefSeq. SOURCE Mucambo virus ORGANISM Mucambo virus Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; Togaviridae; Alphavirus. REFERENCE 1 (bases 1 to 11391) AUTHORS Kinney,R.M., Pfeffer,M., Tsuchiya,K.R., Chang,G.J. and Roehrig,J.T. TITLE Nucleotide sequences of the 26S mRNAs of the viruses defining the Venezuelan equine encephalitis antigenic complex JOURNAL Am. J. Trop. Med. Hyg. 59 (6), 952-964 (1998) PUBMED 9886206 REFERENCE 2 (bases 1 to 11391) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (24-AUG-2018) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 3 (bases 1 to 11391) AUTHORS Kinney,R.M., Pfeffer,M. and Meissner,J. TITLE Direct Submission JOURNAL Submitted (30-JUN-1998) Arbovirus Diseases Branch, Centers for Disease Control and Prevention, P.O. Box 2087, Fort Collins, CO 80522, USA COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AF075253. COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..11391 /organism="Mucambo virus" /mol_type="genomic RNA" /strain="Mucambo BeAn 8" /db_xref="taxon:60875" /country="Brazil" /note="isolated in 1954; sequencing of RT/PCR cDNA amplicons from plaque-purified virus" 5'UTR 1..42 gene 43..7410 /locus_tag="D1R99_gp1" /db_xref="GeneID:37618893" CDS 43..7410 /locus_tag="D1R99_gp1" /note="possible incorporation of arginine, cysteine, or tryptophan at read-through UGA codon in nsP3" /codon_start=1 /transl_except=(pos:5566..5568,aa:OTHER) /product="nonstructural polyprotein" /protein_id="YP_009507798.1" /db_xref="GeneID:37618893" /translation="MEKVHVDIEEDSPFLRALQRSFPQFEVEAKQVTDNDHANARAFS HLASKLIETEVEPSDTILDIGSAPARRMYSKHKYHCVCPMRCAEDPDRLFKYAAKLKK NCKDITDKDLDKKMAELAAVMEDPDLEQDTICLHDDETCRYEGHVAVYQDVYAVDGPT SIYHQANKGVRVAYWIGFDTTPFMFKNLAGAYPSYSTNWADETVLTARNIGLCSSDVM ERSRRGLSILRKKYLKPSNNIIFSVGSTIYHEKRDLLKSWHLPSVFHLRGKQNYTCRC ETIVSCDGYVVKRIAISPGLYGKPSGYAATMHREGFLCCKVTDTLNGERVSFPVCTYV PATLCDQMTGILATDVSAEDAQKLLVGLNQRIVVNGRTQRNTNTMKNYLLPVVAQAFA RWAKEYKDDQDDERPLGLRDRQLVMGCCWAFKKHKITSVYKRPDTQTVIKVPSDFHSF VLPRPGSNTLEIGLRTRIKKLLEDKKIPPPIITPEDVSTAKEAAAEAKEIREAEELRI ALPPIVPDVEEPTLEADVDLMLQEAGAGSVETPRGLIKVTSYSGEDKIGSYAVLSPQA VLRSEKLSCIHPLAEEVIVITHAGRKGRYAVEPYHGKVVVPEGHAIPVADFQALSESA TIVYNEREFVNRYLHHIAINGGALNTDEEYYKVVKPTEYDGEYLYDIDRKQCVKKEQV SGLGLTGELVEPPFHEFAYESLKTRPAAPYQVPTIGVYGVPGSGKSGIIKSAVTKRDL VVSAKKENCAEVIRDVKKMRGLDIVARTVDSVLLNGCKHPVDTLYIDEAFACHAGTLR ALIAIIKPKKAVLCGDPKQCGFFNMMCLRVHFNHEICTQVFHKSISRRCTKSVTSVVS TLFYDKKMRTTNPRETKIEIDTTGSTKPRKDDLILTCFRGWVKQLQIDYKGNEIMTAA ASQGLTRKGVYAVRYKVNENPLYAANSEHVNVLLTRTEDRIVWKTLAGDPWIKTLTAK YTGDFTATLEEWQQEHDAIMKHILERPDPADVFQNKANVCWAKALVPVLKTAGIDLTA EQWNTIVQFRDDKAHSAEIALNQLCVRFFGLDLDSGLFSAPTVPLSIKNNHWDNSPSP NMYGLNKEVARQLSRRYPQLPRAVATGRVLDMNTGTLRGYDPCINLVPVNRRLPHALV THHSEQPRSDFSAFASKLRGRTVLVVGEKLNITGKNVDWLSESPEATFKSRLDLGIPH ELPKYDIVFVNVRTPYKYHHYQQCEDHAIKLSMLTKKACLHLNPGGTCVSIGYGYADR ASESIVGAIARQFKFSRVCKPKVSTEETEILFVFIGYDRKVRTHNPYKLSSTLTNIYT GSHLHEAGCAPSYHVVRGDIANAEEGVIVNAANSRGQPGGGVCGALYKRFPENFDLQP IEVGKSRLVKGAAKHIIHAVGPNFNKVSELDGDKQLAEAYESVAKIINDNHYRTVAIP LLSTGIFAGNKDRLMQSLNHLLTALDTTDADVAIYCRDKKWETTLKEVIARREAVEEI CISEDASVAEPDAELVRVHPKSSLAGRKGFSTVDGKTFSYLEGTKFHQAAKDVAEINA MWPTVTEANEQICLYILGESMSSIRSKCPVEESEASTPPSTLPCLCIHSMTPERVHRL KASRPEQITVCSSFPLPKYRITGVQKIQCAQPILFLPKVPEHIHPRRYLPPQENREEE ENSVETLPESPEIPTVAAASEIDTQTEETMSITSETTVASFVPNEIVVEAEIHSDLMR FSSDWSIPQASDFDVDSVSVQGTVDIFNSALQEDARSAVSSYCSGIKPIPAPRTVFIQ PPKPRRRRRKSKASTSSTAPLVTVSSATSRSSLVSNPPGRKGVITREEFEAFVAQQQX RYEAGAYIFSSDTGQGHLQQKSVRQTALSEVVLERTELENSYAPRLDLIKEESLRKKL QLNPTQANRSRYQSRRVENMKAVTTKRLLGGLGHYLKSEGKVECYRTLYPVPLYSASV NRVFSNPRVAVEACNAMLKENFPTVASYCITPEYDAYMDMVDGASCCLDTASFCPAKL RSFPKRHAYLEPTIRSAVPSAIQNTLQNVLAAATKRNCNVTQMRELPVLDSAAFNVEC FKKYACNNEYWETYKECPIRLTEENVTNYITKLKGPKAAALYAKTHNLSMLQDIPMDR FVMDLKRDVKVTPGTKHTEERPKVQVIQAADPLATAYLCGIHRELVRRLNAVLLPNVH TLFDMSAEDFDAIIAEHFQPGDSVLETDIASFDKSEDDAMALTALMILEDLGVDQELL TLIEAAFGEISSIHLPTKTKFKFGAMMKSGMFLTLFVNTVINIVIASRVLRERLTQSP CAAFIGDDNIVKGVKSDKLMADRCATWLNMEVKIIDAVVGIKAPYFCGGFILCDTVTG TACRVADPLKRLFKLGKPLAADDEHDDDRRRALQEESARWNRVGIHSELCKAVESRYE TTGTSVIITAMTTLAQSVPAFKHLRGNPVTLYG" mat_peptide 43..1647 /locus_tag="D1R99_gp1" /product="methyltransferase nsP1" /note="guanyltransferase" /protein_id="YP_009509023.1" mat_peptide 1648..4029 /locus_tag="D1R99_gp1" /product="nonstructural proteinase nsP2" /note="RNA helicase" /protein_id="YP_009509024.1" mat_peptide 4030..5586 /locus_tag="D1R99_gp1" /product="nonstructural protein nsP3" /protein_id="YP_009509025.1" mat_peptide 5587..7407 /locus_tag="D1R99_gp1" /product="RNA polymerase nsP4" /protein_id="YP_009509026.1" gene 7416..11391 /locus_tag="D1R99_gp2" /db_xref="GeneID:37618894" mRNA 7416..11391 /locus_tag="D1R99_gp2" /product="structural polyprotein" /note="26S mRNA; structural polyprotein is translated from an intracellular subgenomic 26S mRNA" /db_xref="GeneID:37618894" CDS 7446..11210 /locus_tag="D1R99_gp2" /codon_start=1 /product="structural polyprotein" /protein_id="YP_009507799.1" /db_xref="GeneID:37618894" /translation="MFPYQSTMFPMQPAPFRNPYAPPRRPWFPRTDPFLAMQVQELAR SMASLTFKQRRDTPPEGPPAKKKRKEPQQQVAQAQVKKKNGKPKKKKSNGAPPPKNQK STKKKTNKKPGKRQRMVMKLESDKTFPILLDGKINGYACVVGGKLFRPMHVEGKIDNE TLASLKTKKASKYDLEYADVPQSMRADTFKYTHDKPQGYYNWHHGAVQYENGRFTVPK GVGAKGDSGRPILDNQGRVVAIVLGGVNEGSRTALSVVMWNEKGVTVKYTPENCEQWS LVTTMCLLANVTFPCSTPPICYDRAPAETLMMLSKNIDNPGYDELLEAVLKCPGRQKR STEELFKEYKLTRPYMAKCVRCAVGSCHSPIAIEAVRSDGHDGYIRIQTSSQYGLDPS GNVKSRVMRYNLYGKIVEVPLHQVSLHTSRPCHIIDGHGYFLLARCPEGDSITMEFKK DSVTHSCSVPYEVKFTPVGRELYSHPPEHGTEHPCRVYVHDAQQKDAYVEMHLPGSEV DSSLLSMSGRAVRVTPPSGQSVLVECNCGSAVSETINTAKSYSQCTKTSQCRAYRLQN DKWVYNSDKLPKASGETLKGKLHVPYLLSEAKCTVPLAPEPIVTFGFRFVSLKLHPRN PTYLTTRQLDGEPNYTHELISEPTTRNFTVTEHGWEYVWGNHPPQRYWAQETAPGNPH GLPHEVITHYYHRYPMSTIFGLSICAAVVTTSIAASTWLLCKSRVSCLTPYRLTPNAQ LPVCLAFLCCARTARAETTWESLDHLWNHNQQMFWSQLLIPLAALIVVTRLLKCMCCV VPFLVLAGAASVGAYEHATTMPNQVGIPYNTVVNRAGYAPLAISIIPTKIRLIPTLNL EYITCHYKTGLDSPFIKCCGTQECPKVNRPDEQCKVFTGVYPFMWGGAYCFCDSENTQ ISRAYVMKSDDCSADHALAYKAHTASVQAFLNITVGEQSTTAVVYVNGETPVNFNGIK LVAGPLSTAWTPFDRKVVQYAGEIYNYDFPEYGAGHAGAFGDLQARTITSNDLYANTN LVLQRPNTGTIHVPYTQAPSGFEQWKKDKPPSLKYTAPFGCEIHVNPVRAENCAVGFI PLAFDIPDALFTRVSETPTLSSAECSLNECTYSTDFGGIATVKYSASKSGKCAVHVPS GTATLKESLVEVVEQGSMTLHFSTASIHPEFKLQICTKVLTCKGDCHPPKDHIVTHPQ HHAQTFTAAVSKTAWTWLTSLLGGSAVIIIIGLVLATVVAMYVLTNQKHN" mat_peptide 7446..8270 /locus_tag="D1R99_gp2" /product="nucleocapsid protein" /note="capsid" /protein_id="YP_009509027.1" mat_peptide 8271..8447 /locus_tag="D1R99_gp2" /product="polyprotein cleavage product E3" /protein_id="YP_009509029.1" mat_peptide 8448..9713 /locus_tag="D1R99_gp2" /product="envelope glycoprotein 2" /note="E2; main neutralization epitopes" /protein_id="YP_009509028.1" mat_peptide 9714..9881 /locus_tag="D1R99_gp2" /product="6K polyprotein cleavage product" /protein_id="YP_009509030.1" mat_peptide 9882..11207 /locus_tag="D1R99_gp2" /product="envelope glycoprotein 1" /note="E1; contains fusion domain" /protein_id="YP_009509031.1" 3'UTR 11208..11391 /locus_tag="D1R99_gp2" ORIGIN 1 atgggcggcg tatgagagta gcccaaatta aaactaccca ttatggagaa agttcacgtt 61 gatatcgagg aggatagccc cttcctcaga gctttgcaac ggagcttccc ccagtttgag 121 gtagaagcca agcaggtcac agacaatgac catgctaatg ctagagcgtt ttcgcatcta 181 gcttccaaac tgatcgaaac ggaggtagaa ccatccgaca cgatcctaga cattggaagt 241 gcgcccgccc gcagaatgta ttcaaaacac aagtaccatt gtgtctgtcc gatgagatgt 301 gctgaagacc cggacagatt atttaagtat gcagccaagc tgaagaagaa ttgtaaagac 361 ataacagaca aggacctaga caaaaagatg gcagagctcg cagctgtcat ggaggacccc 421 gatttggagc aggataccat atgtttgcat gatgatgaaa cgtgccgcta tgaaggacac 481 gttgctgttt accaggacgt ctatgcggta gacggtccca ccagcattta ccatcaagca 541 aataaaggtg taagagtggc ctattggatc ggctttgata ccacgccgtt tatgtttaaa 601 aatttggccg gagcctatcc ttcctactct acaaattggg ctgacgaaac agtgttgaca 661 gcccgtaaca tcggcttatg cagctcagat gtcatggaaa ggtcccgtag aggtttgtct 721 attttaagga agaaatattt aaaaccatcg aataacatca tattttcagt gggttccacc 781 atctaccatg aaaaaagaga cttgttaaaa agttggcacc taccttctgt gtttcaccta 841 cgtggcaagc agaattacac atgtcggtgt gagacgatag tgagttgcga cgggtacgtc 901 gtaaaacgga tcgctatcag tccaggcctg tatgggaaac cgtcgggcta tgctgccact 961 atgcatcgcg agggattctt gtgctgcaag gtaacagata cgctcaacgg ggagagggtc 1021 tcttttcccg tctgcacgta tgtgccagcg actctatgcg accaaatgac cggcatattg 1081 gctacagatg tcagtgccga agacgcacaa aagctgttgg ttgggctcaa ccaacgcatt 1141 gtcgtaaatg gccgcacaca gagaaatact aatactatga aaaactattt attaccagta 1201 gttgcacagg cttttgccag gtgggctaaa gaatacaaag atgaccaaga tgatgagaga 1261 ccgctagggt tacgggaccg acagctcgtg atgggctgtt gttgggcatt taagaaacac 1321 aagattacat ctgtgtataa acgtcccgac actcaaacgg ttatcaaggt acctagtgac 1381 ttccattcct ttgtgctacc cagacctggt agcaatacat tggaaattgg gcttagaacc 1441 cggataaaga agttactgga agataaaaag ataccaccac ctataattac tccagaagac 1501 gtctcaacag caaaagaagc cgcagcggaa gccaaggaga ttagggaggc tgaggagttg 1561 cgaatcgcac taccccctat agtgccggac gtggaagaac caactctaga agccgacgtg 1621 gacttgatgc tgcaagaggc aggcgctggt tccgtcgaga ctcccagagg attaatcaaa 1681 gttactagct actctggtga ggataagatt ggttcttatg ccgtgttgtc gcctcaagcc 1741 gtcctgagaa gtgaaaaact gtcctgcata cacccccttg cggaggaagt catcgtaata 1801 acgcacgcag gaaggaaagg acgctatgct gttgagccgt atcatggcaa agtggtggtc 1861 cctgaaggac atgcgatccc tgtagcagac tttcaggctc tcagtgagag cgctactatt 1921 gtgtacaacg aacgagagtt tgtgaacagg tacttgcatc acatcgccat taatggagga 1981 gccttgaata ccgatgaaga gtactataaa gtggtcaaac ctaccgagta tgatggagag 2041 tacttgtatg atattgacag gaaacagtgt gtcaagaaag aacaggtgtc tggcctcggc 2101 ctgacaggtg agctggtaga gcctccattt cacgaattcg cgtatgaaag cctgaagacc 2161 agaccggcag ctccatatca agtgcctacc ataggagtgt acggagtacc aggctcgggt 2221 aagtcaggaa ttataaaaag tgccgttacc aagagagatc tggtggtcag cgcaaagaag 2281 gaaaattgcg ccgaggttat ccgcgacgtg aagaaaatgc gaggcttaga catagtggca 2341 cgcactgtag attcagtgct gctgaatggc tgcaagcacc cagttgatac actgtacatt 2401 gatgaggctt ttgcttgtca tgccgggacc ctccgcgctt tgattgcaat tattaaacca 2461 aaaaaggcag ttctctgcgg agacccgaag cagtgcggct tctttaatat gatgtgcctc 2521 agagtgcatt tcaatcacga aatttgcacg caggtgttcc ataaaagcat atcccgtagg 2581 tgcactaaat cggtcacatc cgtggtgtct acgctgtttt atgacaagaa gatgcgcact 2641 actaatccca gggagacaaa aattgaaatt gacaccacag ggagtacaaa gcctagaaaa 2701 gatgatctca tattgacgtg cttcagggga tgggtaaagc agctgcaaat tgactacaag 2761 ggcaatgaaa tcatgacggc ggcagcctca cagggattaa cccgaaaagg ggtttatgcc 2821 gtcaggtata aagtcaatga gaatccccta tacgccgcaa attcagagca cgtcaacgtc 2881 ctactcactc gtacagagga taggattgtg tggaaaaccc tggcaggaga tccttggatt 2941 aaaactctta cggccaagta caccggtgac tttactgcta ccttggaaga atggcaacaa 3001 gagcatgacg ccattatgaa gcacattctg gaaagacctg atcctgcgga cgtgttccag 3061 aataaagcca atgtgtgctg ggctaaagcc ttagtacccg tactgaagac tgcaggtatt 3121 gacctaacgg cagagcagtg gaacaccatt gtccaattca gagatgacaa ggctcactcg 3181 gcagaaatag cactcaacca attgtgtgta cggttctttg gactagattt ggattcaggg 3241 ttgttttctg cccccactgt gccgttgtct ataaaaaata accactggga taattcacct 3301 tcacccaata tgtacgggtt gaataaggaa gtagcgcgac aactttctcg acgctacccg 3361 cagcttcctc gtgcagtggc gactggcaga gttttggaca tgaatactgg gaccctgcgt 3421 ggttacgacc catgcattaa tcttgtgccc gttaatcgga ggctaccgca tgcattggta 3481 acgcaccatt cagagcaacc acgcagcgat ttttcggcat ttgccagcaa gttgaggggt 3541 aggactgtcc tggttgtagg agagaaacta aacataaccg ggaaaaatgt tgactggcta 3601 tccgaaagtc ccgaagccac tttcaaatcg cgcctggatc tcggtatccc gcacgaactg 3661 cccaaatatg acattgtgtt tgtgaatgtc cggacacctt acaagtacca tcactaccag 3721 cagtgtgaag accatgcgat caagctcagt atgttgacaa agaaagcgtg cttacatctt 3781 aatcctggtg gaacctgtgt cagcattggc tatggctacg cagatcgagc cagtgaaagc 3841 attgtcggag ctattgcaag gcagtttaag ttctcccgag tgtgtaagcc aaaagtatca 3901 acggaggaga cagagatact atttgtcttc atagggtatg accggaaggt tcgtacacac 3961 aatccctata aattgtcttc aacattgacc aacatttaca caggctcaca cttacatgag 4021 gcaggctgcg ctccatccta ccatgtagtg cgtggtgata ttgccaatgc tgaggaggga 4081 gtaattgtca acgccgctaa tagcagaggg caacctgggg gtggggtatg tggagctctt 4141 tacaaacgct tcccggagaa cttcgacctg cagcccatag aggtcggcaa gtcaaggctg 4201 gttaaagggg cggctaaaca tatcattcat gcagtaggac ccaattttaa taaggtgtcg 4261 gaacttgatg gggacaagca attggcagaa gcttatgaat cagtagctaa aatcattaat 4321 gataatcatt accgcaccgt ggcaattccg ctgctgtcga caggtatttt tgccggaaac 4381 aaagacagac tcatgcagtc attaaatcac ctgcttacgg cattagacac taccgatgca 4441 gatgtagcta tatactgcag agataagaag tgggaaacta ctctgaagga agttatagca 4501 cggagagagg ctgtcgaaga gatatgtatc tctgaagacg catccgtagc agaacctgac 4561 gcagagctgg taagagtaca tcccaaaagt tcgctggcag gacgcaaagg ctttagcacg 4621 gtcgacggga aaacattttc atatttggaa gggactaaat ttcatcaggc agcgaaggat 4681 gtggccgaga ttaatgcgat gtggcccaca gttaccgagg ctaatgagca aatatgcctg 4741 tacattttgg gcgaaagtat gagcagcatc cgatcgaagt gccccgttga agaatctgag 4801 gcgtcaactc cacccagcac gttgccgtgt ctctgtatcc attctatgac accggaacga 4861 gtacacaggc ttaaggcctc acgtccagag caaataacgg tgtgttcgtc gtttccccta 4921 cccaagtaca gaatcaccgg ggtgcagaag attcaatgcg ctcagcctat actcttcctc 4981 ccgaaagtgc cggaacatat ccatccacgc aggtatcttc ccccgcagga gaacagagag 5041 gaagaggaga attctgtgga aacgctgcct gaatcgcctg aaattcctac agttgctgcc 5101 gcctctgaaa ttgacaccca aacggaagag accatgagca taacgtcgga aacgactgtt 5161 gcctcgttcg tgcctaacga aattgtggtc gaggcggaaa tacactcgga tctcatgcga 5221 ttctcctctg actggtccat tcctcaagcc tctgactttg acgttgacag cgtgtcggtt 5281 caaggtactg tggacatatt taacagtgcg ctgcaggagg atgcgcgtag cgccgtatca 5341 tcttactgct cgggaataaa acccattccc gcgccacgta ccgtttttat ccagccaccg 5401 aaacctcgca gaagacgtcg taagtcgaag gccagtacca gttctacggc tcccttggta 5461 actgtgtcca gcgccacttc gagatctagt ttggtgtcta acccacccgg aagaaaaggg 5521 gttattacca gagaggagtt cgaagccttc gtcgcgcaac aacaatgacg gtacgaagcg 5581 ggtgcgtaca tcttctcctc cgacaccggt caagggcatc tacaacaaaa atctgtgagg 5641 caaacagcgc tgtccgaagt ggtgcttgaa cggacggagt tggagaattc gtacgccccg 5701 cgcctcgact taataaaaga ggaaagttta cgtaagaaat tgcaattgaa tccgacacag 5761 gctaatagga gcaggtatca atccagaaga gtggagaata tgaaagcagt gaccaccaaa 5821 agactactcg gcggactggg acattacttg aagtctgaag gaaaagttga atgttaccgt 5881 acattgtacc ccgtcccctt gtattcagca agcgttaacc gcgttttctc gaatcctcga 5941 gtggcagtcg aagcgtgtaa tgctatgcta aaggagaact ttccaacagt ggcttcgtac 6001 tgcataacac cagagtacga tgcgtacatg gacatggtgg acggcgcctc ttgttgttta 6061 gacactgcga gtttttgccc tgccaaattg cgtagttttc ctaagagaca tgcttatttg 6121 gagccgacca ttcgctcagc agtgccgtct gctatacaaa ataccttgca gaatgtacta 6181 gcagcggcca ccaaacggaa ctgcaatgtt acacaaatga gagagctacc tgtgttggat 6241 tccgccgcct tcaatgtaga gtgctttaag aagtatgcat gcaataatga atattgggaa 6301 acatataagg aatgtcctat tagattaact gaagagaatg tgactaatta tattactaaa 6361 ttgaaaggtc cgaaggcggc tgctttgtat gctaaaaccc acaaccttag catgcttcag 6421 gacattccca tggatagatt tgttatggat ttaaaaagag atgtgaaggt gacacctggt 6481 accaaacata cggaggagag acccaaggta caggtaatcc aggcagcaga tccattggcc 6541 acagcctact tatgcgggat tcatcgtgaa ctggtgcgga ggttaaacgc agttttactg 6601 ccgaacgtcc ataccttgtt cgacatgtcc gctgaggatt tcgatgccat tattgctgaa 6661 catttccaac ctggggacag tgtgttggag accgacatcg cttcgttcga taagagcgag 6721 gacgatgcga tggcactgac agcattgatg atcctggaag atttaggcgt agaccaagag 6781 ttgcttacgt tgattgaagc cgcattcgga gaaatatcat ccatccacct tcccacgaaa 6841 actaaattta aatttggtgc tatgatgaaa tctggtatgt ttcttaccct gtttgtgaat 6901 acagttatta atattgttat agccagcaga gtgctccgag aacgcctgac acagtcgcct 6961 tgcgctgcat ttatcggcga tgacaacatt gttaaaggcg ttaaatcgga taagttgatg 7021 gccgacaggt gtgccacgtg gttgaacatg gaggtaaaaa ttattgatgc cgtagtagga 7081 ataaaggccc cgtacttttg cggcgggttt atcctgtgtg acacggtaac cggcacggca 7141 tgtcgtgtgg ccgatccttt gaagagactg tttaaactcg ggaaacctct cgcggctgac 7201 gatgaacacg atgacgatcg tcgccgcgct ctgcaggagg aatcagcccg ctggaaccgc 7261 gtaggcatac actctgaatt atgcaaggcg gtggagtcac gttacgagac cacaggcacg 7321 tccgtgatta taacagctat gaccaccttg gcacagagtg ttcctgcgtt caagcacctg 7381 agaggaaacc cagtaactct ctacggctga cctgaatgga ctgtaacgta gttcagtccg 7441 caaccatgtt cccttaccaa tccacaatgt ttccaatgca accagcgcct tttcgcaacc 7501 cgtacgctcc tcctagaaga ccgtggttcc ctagaaccga tcccttctta gccatgcagg 7561 tgcaggagtt ggcccgatca atggcgagct tgacgtttaa acagcgtcga gatacgccac 7621 ccgaggggcc acctgctaag aagaagcgta aggagcctca acagcaggta gctcaggcgc 7681 aggttaagaa aaagaacgga aaaccgaaga agaagaaaag taacggagca ccacccccaa 7741 aaaatcagaa gagcaccaag aagaagacca ataagaaacc tggaaaaaga caacggatgg 7801 ttatgaagtt agaatcagac aaaacatttc ctattttgct ggatggaaaa attaatggat 7861 atgcttgcgt tgttggagga aagctatttc gacccatgca cgtggaaggc aaaattgaca 7921 atgaaactct tgcctccctg aagacgaaga aggcatccaa atacgaccta gagtatgccg 7981 atgttcctca aagcatgcga gcagatacct ttaaatacac acatgataag cctcaagggt 8041 attataattg gcatcacggc gccgtgcagt atgaaaatgg gagattcacg gtgccgaaag 8101 gtgtgggagc gaaaggagac agtggacgcc ccatactaga taatcaaggc agagtcgtgg 8161 ccattgtgct gggaggggtg aatgaaggat ccaggacagc tttgtccgta gttatgtgga 8221 atgaaaaagg ggtcaccgta aaatatacac cagaaaactg tgagcaatgg tcactagtta 8281 ccaccatgtg tcttctcgcc aatgttacgt tcccttgttc cactccacca atttgctacg 8341 accgagcacc cgcagaaacc ctgatgatgc ttagcaagaa cattgacaat cctggctatg 8401 atgaattgct ggaagcagtg ctgaaatgcc ccggcagaca gaagagatct acggaggagt 8461 tatttaagga gtacaaactt acacgtccgt acatggccaa gtgtgtgcgg tgtgccgttg 8521 gaagttgcca cagcccgatc gctatagaag cagtaagaag cgacgggcat gacggctaca 8581 tccgaataca gacatcatca cagtacggtt tagacccctc cgggaacgtt aagagcagag 8641 ttatgaggta taatctgtat ggcaagatcg tagaagttcc attacatcag gtttcattac 8701 acacatctcg gccttgccac attattgatg gtcacggata tttcctcctc gcacgctgcc 8761 cagagggcga ctctatcacc atggagttta agaaggattc cgtcacccat tcctgttcag 8821 tgccttatga agtgaaattc acacccgtgg gcagagaatt atatagccat cccccagaac 8881 acggcacaga acatccgtgc cgtgtgtatg tccacgacgc ccagcaaaaa gatgcgtatg 8941 tggagatgca cctgcccggg tccgaagttg acagttccct gctctccatg agcggtagag 9001 cggtccgggt aacaccacca tcagggcaaa gtgtccttgt ggagtgcaac tgtggctccg 9061 ctgtgtcaga aaccataaac actgcaaaat catacagcca atgcacaaaa acatcacaat 9121 gccgcgcgta ccgtctgcag aatgataagt gggtatacaa ttcagacaaa cttccaaagg 9181 catcgggaga aacgctgaaa gggaaactgc atgtacctta tctcctttcc gaagcgaagt 9241 gtaccgtacc tttagcaccc gagccaatag taaccttcgg ctttcgattc gtatctttga 9301 aattgcatcc acggaatccg acatatttga ctacccgcca gctagatgga gaaccgaatt 9361 acacccacga gctaatttca gaaccaacaa ctagaaattt tacagtgact gagcatggat 9421 gggaatacgt ttggggtaat cacccgcctc agaggtactg ggcacaggag acagctccag 9481 gcaacccgca tgggctgccg cacgaggtga ttactcatta ctatcacagg tacccaatgt 9541 ccacgatttt cggactatcg atttgcgccg cagtggtaac cacctcaata gccgcatcca 9601 cctggctgtt gtgcaagtcg agagtatctt gtttgactcc gtatcgactg accccgaatg 9661 ctcagttacc tgtgtgtcta gccttcctgt gctgcgcgag gacagcccgt gcagagacca 9721 catgggaatc actagaccat ttatggaacc ataatcaaca gatgttctgg agtcaactgc 9781 tcattcccct agccgcgctc attgtggtga cccgcttgct gaagtgcatg tgttgcgtcg 9841 ttcctttttt agtcctagca ggcgccgcaa gcgtcggcgc ctacgaacac gcaaccacga 9901 tgccgaatca ggtggggatc ccgtataata cagtagtcaa ccgcgcaggg tacgcaccat 9961 tggcaatcag cattattcca accaagatac ggctaattcc tactttgaat ttagaatata 10021 ttacatgcca ctataagaca ggattagatt cacctttcat taaatgctgc ggaacgcagg 10081 aatgccccaa agtgaataga cccgatgaac agtgtaaagt cttcacgggg gtgtatccgt 10141 ttatgtgggg aggcgcctac tgcttctgtg actctgaaaa cacgcaaatt agtcgagcgt 10201 atgtgatgaa atcagatgac tgctcagctg accacgcctt ggcctacaaa gctcatactg 10261 cctcagtcca agcttttctg aatataactg tgggagagca atcgacgaca gcggtagtgt 10321 acgtgaatgg agaaacaccg gtcaatttta acggcattaa attggttgca ggccctttat 10381 caactgcctg gaccccattt gatcggaaag tggtgcagta cgccggagag atctacaatt 10441 atgacttccc ggagtacgga gctgggcatg caggggcgtt cggggatctt caagccagaa 10501 caatcaccag taatgacctg tacgccaaca cgaatttagt attgcaaaga cccaacacag 10561 gcaccatcca tgttccttac acgcaggcac cgtcaggctt tgagcagtgg aagaaagaca 10621 aaccaccatc attaaagtac accgcaccat ttgggtgcga aattcatgtg aatcccgtca 10681 gagctgagaa ttgcgcagta ggatttatac cattagcctt cgacataccc gatgccttgt 10741 ttaccagggt gtcagaaaca ccgacgttgt cgagcgctga gtgctccttg aatgagtgta 10801 catactcaac ggactttggc gggatcgcca ctgtcaagta ctcggctagt aaatcaggca 10861 aatgcgcagt acatgttccc tcaggcactg caactctgaa agagtcattg gtggaagtgg 10921 tcgaacaagg gtcaatgacc cttcactttt cgaccgccag tatacaccca gagtttaaat 10981 tgcagatctg tacgaaggta ctcacatgta aaggcgactg tcatccgcct aaagaccata 11041 ttgtgacgca cccccagcac cacgcccaga catttacagc tgcggtatcc aagaccgctt 11101 ggacgtggtt aacgtcactc ttaggagggt cagcagtaat tattataatt ggccttgtat 11161 tagcaactgt tgttgctatg tatgtgctga ccaaccagaa acataattag accacagcag 11221 cgattggaaa gctgcctatt agaaacatgt agcggcaatt ggcaagccgc ctataaatgt 11281 ttagcagcaa ttggcaagct gcatatataa attacctagc ggcaattggc acgccgctta 11341 taaaattttt attttctttt accaataatt ggattttgtt tttaatattt c //