LOCUS NC_038675 11530 bp RNA linear VRL 24-AUG-2018 DEFINITION Tonate virus strain Tonate CaAn 410d, complete genome. ACCESSION NC_038675 VERSION NC_038675.1 DBLINK BioProject: PRJNA485481 KEYWORDS RefSeq. SOURCE Tonate virus ORGANISM Tonate virus Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; Togaviridae; Alphavirus. REFERENCE 1 (bases 1 to 11530) AUTHORS Kinney,R.M., Pfeffer,M., Tsuchiya,K.R., Chang,G.J. and Roehrig,J.T. TITLE Nucleotide sequences of the 26S mRNAs of the viruses defining the Venezuelan equine encephalitis antigenic complex JOURNAL Am. J. Trop. Med. Hyg. 59 (6), 952-964 (1998) PUBMED 9886206 REFERENCE 2 (bases 1 to 11530) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (24-AUG-2018) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 3 (bases 1 to 11530) AUTHORS Kinney,R.M., Pfeffer,M. and Meissner,J. TITLE Direct Submission JOURNAL Submitted (30-JUN-1998) Arbovirus Diseases Branch, Centers for Disease Control and Prevention, P.O. Box 2087, Fort Collins, CO 80522, USA COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AF075254. COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..11530 /organism="Tonate virus" /mol_type="genomic RNA" /strain="Tonate CaAn 410d" /db_xref="taxon:60877" /country="Guyana" /note="isolated in 1973; sequencing of RT/PCR cDNA amplicons from plaque-purified virus" 5'UTR 1..43 gene 44..7549 /locus_tag="D1S02_gp1" /db_xref="GeneID:37618903" CDS 44..7549 /locus_tag="D1S02_gp1" /note="possible incorporation of arginine, cysteine, or tryptophan at read-through UGA codon in nsP3" /codon_start=1 /transl_except=(pos:5705..5707,aa:OTHER) /product="nonstructural polyprotein" /protein_id="YP_009507804.1" /db_xref="GeneID:37618903" /translation="MEKVHVDIEEDSPFLRALQRSFPQFEVEAKQVTDNDHANARAFS HLASKLIETEVEPSDTILDIGSAPARRMYSNHKYHCICPMKCAEDPDRLYKYASKLKK NCKDITDKELDKKMAELAAVMEDPDLEQETICLHDDESCRYEGNVAVYQDVYAVDGPT SIYHQANKGVRVAYWIGFDTTPFMFKNLAGAYPSYSTNWADETVLTARNIGLCSSDVM ERSRRGLSILRKKYLKPSNNVIFSVGSTIYHEKRDLLKSWHLPSVFHLRGKQNYTCRC ETIVSCDGYVVKRIAISPGLYGKPSGYAATMHREGFLCCKVTDTLNGERVSFPVCTYV PATLCDQMTGILATDVSAEDAQKLLVGLNQRIVVNGRTQRNTNTMKNYLLPVVAQAFA RWAKEYKEDQDDERPLGLRDRQLVMGCCWAFKKHKITSVYKRPDTQTVIKVPSDFHSF VLPRLGSSSLEMGLRTRIKKLLEDKKTTPPIITPEDISSAKDAATEAKEIKEAEELRI ALPPMVPDVEEPTLEADVDLMLQEAGAGSVETPRGLIKVTSYSGEDKIGSYAVLSPQA VLRSEKLSCIHPLAEEVIVITHAGRKGRYAVEPYHGKVVVPEGHAIPVADFQALSESA TIVYNEREFVNRYLHHIAINGGALNTDEEYYKVVKPNEHDGEYLYDIDKKQCVKKEQV SGLGLAGELVEPPFHEFAYESLRTRPAAPYQVPTIGVYGVPGSGKSGIIKSAVTKRDL VVSAKKENCTEIIRDVKKMRDLDIVARTVDSVLLNGCKHPVDTLYIDEAFACHAGTLR ALIAIIKPKKAVLCGDPKQCGFFNMMCLKVHFNHEICTQVFHKSISRRCTKSVTSVVS TLFYDKRMRTTNPKETKIEIDTTGSTKPKKDDLILTCFRGWVKQLQIDYKGNEIMTAA ASQGLTRKGVYAVRYKVNENPLYAPSSEHVNVLLTRTEDRIVWKTLAGDPWIKTLTAR YVGDFTATLEEWQQEHDAIMKHILEKPDPTDVFQNKANVCWAKALVPVLKTAGIDLTT DQWNTVVHFKEDKAHSAEIVLNQLCVRFFGLDLDSGLFSAPTVPLSIKSNHWDNSPSP NMYGLNKDVVRQLSVRYPQLARATATGRVFDMNTGTLRSYDPRVNLVPVNRKLPHALV THRSEQPRSDFSTFVSKLRGRTVLVVGDKMNITGKTVDWLSENPEATFKSRLDLGIPN GLPKYDIVFVNVRTPYKYHHYQQCEDHAIKLSMLTKKACLHLNPGGTCVSIGYGYADR ASESIIGAIARQFKFSRVCKPKASLEETEVLFVFIGYDRRVRTHNPYKLSSTLTNIYT GSHLHEAGCAPSYHVVRGDIANAEEGVIVNAANSRGQPGGGVCGALYKRFPESFDMQP IEVGKSRLVKGAAKHIIHAVGPNFNKVSELEGDKQLAEAYESVAKIINDNHYRTVAIP LLSTGIFAGNKDRLMQSLNHLLTALDTTDADVAIYCRDKKWEATLKEVIARREAVEEI CISEDASIAEPDAELVRVHPKSSLAGRRGFSTTDGKTFSYLEGTKFHQAAKDVAEINA MWPNATEANEQICLYILGESMSSIRSKCPVEESEASTPPSTLPCLCIHSMTPERVHRL KASRPEQITVCSSFSLPKYRITGVQKIQCSHPILFLPKVPEYIHPRRYLPQTENPVDP PELPEPSAVATPTADGQTEEAMSIMSETTVASLVPNEIEPSTAATPAADSQTEETMSI MSETTVASFVPNEIVVEAQVHSDLMRFSSSWSIPQASDFDVDSVSVQGTVDIFNSALQ EDASSVASSYCSGIKPVPAPRTVFTQSPKPRRRRRRSKASTSSTAPLVTTSRATSRSS LVSNPPGEKVTTSRATSRSSLVSNPPGEKRVITREEFEAFVAQQQXRYEAGAYIFSSD TGQGHLQQKSVRQTTLSEVVLERTELEHSYAPRLDLEKEEILRKKLQLNPTPANRSRY QSRRVENMKAVTTKRLLGGLGSYLKTEGKVECYRTLYPVPEYSASVNQVFSSPRVAIE ACNAVLKENFPTVTSYCITPEYDAYMDMVDGASCCLDTASFCPAKLRSFPKRHAYLEP TIRSAVPSAIQNTLQNVLAAATKRNCNVTQMRELPVLDSAAFNVECFKKYACNNEYWE TYREHPIRLTEENVTNYITKLKGPKAAALYAKTHNLSMLQDIPMDRFVMDLKRDVKVT PGTKHTEERPKVQVIQAADPLATAYLCGIHRELVRRLNAVLLPNVHTLFDMSAEDFDA IIAEHFQPGDSVLETDIASFDKSEDDAMALTALMILEDLGVDPELLTLIEAAFGEISS IHLPTKTKFKFGAMMKSGMFLTLFINTVINIVIASRVLRERLTNSPCAAFIGDDNIVK GIKSDKLMADRCATWLNMEVKIIDAVVGTKAPYFCGGFILCDTVTGTACRVADPLKRL FKLGKPLAADDEHDDDRRRALHEESARWNRVGIHSELCKAVESRYETTGTSVIITAMT TLAQSVSAFKHLRGNPLTLYG" mat_peptide 44..1648 /locus_tag="D1S02_gp1" /product="methyltransferase nsP1" /note="guanyltransferase" /protein_id="YP_009509050.1" mat_peptide 1649..4030 /locus_tag="D1S02_gp1" /product="nonstructural proteinase nsP2" /note="RNA helicase" /protein_id="YP_009509051.1" mat_peptide 4031..5725 /locus_tag="D1S02_gp1" /product="nonstructural protein nsP3" /protein_id="YP_009509052.1" mat_peptide 5726..7546 /locus_tag="D1S02_gp1" /product="RNA polymerase nsP4" /protein_id="YP_009509053.1" gene 7555..11530 /locus_tag="D1S02_gp2" /db_xref="GeneID:37618902" mRNA 7555..11530 /locus_tag="D1S02_gp2" /product="structural polyprotein" /note="26S mRNA; structural polyprotein is translated from an intracellular subgenomic 26S mRNA" /db_xref="GeneID:37618902" CDS 7585..11349 /locus_tag="D1S02_gp2" /codon_start=1 /product="structural polyprotein" /protein_id="YP_009507805.1" /db_xref="GeneID:37618902" /translation="MFPYQPTMFPMQPAPFRNPYAAPRRPWFPRTDPFLAMQVQELAR SMANLTFKQRRGAMPGGPPAKKKRKEPQQQVTQAQDKKKNGKMKKKKRNGAAPPKNKN SPKKKTNKKPGKRQRMVMKLESDKTFPIMLDGKINRYACVVGGKLFRPMHVEGKIDND TLAALKMKKASKYDLEYADVPQNMRSDTLKYTHEKPQGYYNWHHGAVQYENGRFTVPK GVGAKGDSGRPILDNQGRVVAIVLGGANEGSRTALSVVMWTEKGVTVKYTPENCEQWS LVTTLCLLANVTFPCSTPPICYDRAPAETLTMLSTNIENPGYDELLEAVLKCPGRQKR STEALFKEYKLTRPYMAKCVRCAVGSCHSPIAIEAVRSDGHDGYIRLQTSSQYGLDPA GNVKSRVMRYNLYGKIVEIPLHEVSLHTSRPCHIIDGHGYFLLARCPEGDSITMEFRK DSVTHSCSVPYEVKFTPVGRELYSHPPEHGAEHPCRVYAHDAQQKDAYVEMHLPGSEV DSSLLSMSGSAVRVTPPSGQNVLVECNCGSAVSETISTAKTYNQCTKTSQCRAYRLQN DKWVYNSDKLPKASGETLKGKLHVPYLLSDAKCTVPLAPEPIVTFGFRSISLKLHPRN PTYLTTRQLDGEPNYTHELISEPSIRNFTVTEHGWEYVWGNHPPQRYWAQETAPGNPH GLPHEVIIHYYHRYPMSTTLGLSICAAVVITAIAASTWLFCKSRVSCLTPYRLTPNAQ LPVCLAFLCCARTARAETTWESLDHLWNHNQNMFWSQLLIPLAALIVLTRLLKCLCCV VPFLVLAGAASVGAYEHATTMPNQAGMPFNTVVNRAGYAPLVISITPTKVRLIPTLNL EYVTCHYKTGLDSPSIKCCGTQECPKVNRPDEQCKVFTGVYPFMWGGAYCFCDSENTQ LSRAYVTKSDDCSADHALAYKAHTASVQAFLNITVGEQSTTAVVYVNGETPISFNGIR LVAGPLSTAWTPFDRKVVQYAGEIYNYDFPEYGAGHAGAFGDLQARTTTSNDLYANTN LVLQRPTAGTIHVPYTQAPSGFEQWKKDKPPSLKYTAPFGCEIYVNPVRAENCAVGSI PLAFDIPDALFTRVSETPTLSNAECSLNECVYSSDFGGIATVKYTASKSGKCAVHIPS GTATLKESLVDVVEQGSLTLHFSTANIHPEFKLQICTTHLTCKGDCHPPKDHIVTHPQ LHAQTFTAAVSKTAWTWLTSLLGGSAVIIIIGLVLATVVAMYVLTNQRHN" mat_peptide 7585..8409 /locus_tag="D1S02_gp2" /product="nucleocapsid protein" /note="capsid" /protein_id="YP_009509054.1" mat_peptide 8410..8586 /locus_tag="D1S02_gp2" /product="polyprotein cleavage product E3" /protein_id="YP_009509055.1" mat_peptide 8587..9852 /locus_tag="D1S02_gp2" /product="envelope glycoprotein 2" /note="E2; main neutralization epitopes" /protein_id="YP_009509056.1" mat_peptide 9853..10020 /locus_tag="D1S02_gp2" /product="6K polyprotein cleavage product" /protein_id="YP_009509057.1" mat_peptide 10021..11346 /locus_tag="D1S02_gp2" /product="envelope glycoprotein 1" /note="E1; contains fusion domain" /protein_id="YP_009509058.1" 3'UTR 11347..11530 /locus_tag="D1S02_gp2" ORIGIN 1 atgggcggcg tatgagagaa gcccaaaacc tagactaccc ataatggaga aggttcacgt 61 tgacatcgag gaggacagcc ccttcctcag agcactacaa cggagctttc cgcagtttga 121 ggtagaagcc aagcaggtca cggataatga ccatgctaat gctagagcgt tttcgcatct 181 agcttccaaa ctgatcgaga cggaggtgga accatccgat acgatcctag acattggaag 241 tgcgcccgcc cgcagaatgt attcaaatca caagtaccat tgtatctgtc cgatgaagtg 301 tgcagaagac ccggacagat tatacaaata tgcatctaaa ttgaagaaga actgtaaaga 361 tattacagat aaagaattgg acaagaaaat ggcagagctt gcagcggtca tggaggaccc 421 ggatctagag caggaaacca tttgtttgca tgatgatgaa tcatgccgct atgaaggcaa 481 cgtggcggta tatcaggacg tctacgctgt agatggtccc actagtatat accatcaggc 541 aaacaaaggc gttagagtcg cctactggat aggctttgat actacgccat ttatgttcaa 601 aaatttagct ggagcctacc cttcctactc tactaactgg gccgacgaga ccgtgctgac 661 agcccgtaac atcggcttat gcagctcaga tgtcatggaa cggtctcgtc ggggactgtc 721 cattttaagg aagaagtatt tgaaaccatc aaacaatgtc atattttccg tgggttctac 781 catctaccac gagaaaagag acttattaaa aagctggcac ctaccttctg tatttcacct 841 acgtggcaag caaaattaca catgtcggtg tgagacgata gtgagctgcg acgggtacgt 901 cgttaagcgt atcgctatca gtcctggcct gtacgggaag ccgtcaggct atgctgccac 961 tatgcaccgc gagggattct tgtgctgcaa ggtaacagac acgttgaacg gggagagggt 1021 ctctttccca gtttgtactt atgtgccagc taccctatgt gaccagatga ctggcattct 1081 ggcaacagat gtcagcgcgg aagacgcgca aaagctgctg gttgggctca accagcgcat 1141 tgtcgtgaat ggccgcactc agaggaatac taataccatg aagaattact tactaccagt 1201 agttgcacag gcatttgccc ggtgggctaa ggagtacaaa gaggaccaag atgatgaaag 1261 accgctggga ttgagggaca gacaacttgt gatgggttgc tgctgggcgt ttaagaagca 1321 caagattaca tccgtgtaca aacgccctga cacccagaca gtcataaagg tacccagtga 1381 cttccactcc tttgtgctac ccagattggg gagcagttct ctggagatgg ggctaagaac 1441 tagaataaag aagttgctgg aagataagaa gacaacccca cctataatta ctcctgaaga 1501 catctcatcg gcaaaagatg ctgccacgga agctaaggaa atcaaggagg cagaagagtt 1561 acgtattgca ctgcctccta tggttccaga cgtggaggaa ccgactctgg aagccgatgt 1621 agatttgatg ctgcaagaag caggggcagg gtcagtagag acgccccgag gactaattaa 1681 agttaccagc tattccggtg aagacaagat cggctcctat gcggtacttt caccccaggc 1741 tgtccttcgg agtgagaaac tctcctgcat acacccgctg gcggaagagg ttattgttat 1801 aacccacgcg ggaaggaaag gaaggtatgc agttgaacct tatcacggca aagtggtggt 1861 ccctgaaggg cacgctattc ccgtagcaga cttccaggca ctcagtgaga gcgccaccat 1921 tgtgtataac gagcgggagt ttgtgaacag gtacctacac cacatcgcca tcaacggagg 1981 agccttgaat accgatgaag agtactacaa agtggtcaaa cccaatgaac atgatggaga 2041 atacctatat gacattgata aaaaacagtg tgttaagaag gaacaggtgt ccggcctcgg 2101 gctggcaggt gaactggtgg agcctccgtt ccacgaattc gcgtatgaga gcctaaggac 2161 tagaccggca gctccttatc aagtaccaac tataggagtg tacggagtac ctggctcggg 2221 taagtccggg attataaaaa gtgccgtgac aaaaagagat ctggtggtca gtgcaaagaa 2281 ggaaaattgt accgagatca tacgcgacgt gaagaagatg cgcgatctag acatagtggc 2341 gcgtactgtc gattcggtac tgctgaatgg ctgtaaacac ccagttgaca cgctgtacat 2401 tgatgaggcc ttcgcatgtc acgcaggaac gctacgtgcg ttgattgcca tcattaaacc 2461 taaaaaggca gttctctgcg gagatccgaa acagtgtggc ttttttaaca tgatgtgcct 2521 taaagtgcat tttaatcatg aaatttgcac gcaggtgttc cacaagagca tctctcgtag 2581 gtgcactaaa tcggttacgt ctgttgtgtc cacgctgttt tatgacaaaa gaatgcgaac 2641 caccaaccca aaggagacca aaatcgaaat tgacactacc ggaagtacta agcctaagaa 2701 ggacgacttg atcttgacgt gcttcagagg atgggtgaag caattgcaaa ttgattacaa 2761 gggcaatgaa atcatgacgg ctgcagcctc gcaaggcttg acccggaaag gagtctacgc 2821 tgtcagatat aaagtcaatg agaatccctt gtacgcacct agctcagagc acgtcaatgt 2881 cctgcttacc cgcacagagg ataggattgt gtggaagacc ttggctggag atccctggat 2941 taagactctc actgccagat acgttggcga cttcaccgcc actctagaag agtggcaaca 3001 agagcatgat gccatcatga agcacatctt ggagaagcct gaccctacag acgtgtttca 3061 gaacaaagct aacgtatgct gggccaaggc cttagtacca gtgctgaaga ccgccggtat 3121 tgatttgaca acagaccaat ggaacactgt agttcatttt aaggaagaca aagctcattc 3181 ggcagagatc gtactaaacc agctatgcgt acgattcttc ggactggatt tggactcagg 3241 tttgttctcc gcacccaccg taccactgtc aataaagagc aaccattggg acaattcgcc 3301 ctcaccgaac atgtatgggt tgaacaagga cgtggtgcgc caactttcag tgcgttaccc 3361 tcagcttgcc cgagcaacag cgacaggcag agtattcgat atgaataccg ggacgctgcg 3421 aagctatgac ccgcgtgtca atctagtgcc cgtcaatcgc aagctgccgc acgcgctggt 3481 tacacaccgc tcggagcagc cccgtagtga tttttctacg tttgttagta agttgagggg 3541 tcgaacagtc ctggttgtgg gagataagat gaatatcact ggtaagacag tcgactggct 3601 gtctgaaaat cctgaggcca ccttcaaatc ccgtctagac cttggcatcc caaatgggct 3661 acccaaatat gacatcgtgt ttgtgaacgt tcggacgccc tacaagtatc atcactacca 3721 gcaatgtgaa gaccatgcga taaagctcag catgctgaca aagaaagcgt gcttgcatct 3781 caaccccgga ggaacctgcg tcagcatcgg ttatggctat gcagacaggg ccagtgaaag 3841 catcatcggg gctatcgcac ggcagtttaa gttttctaga gtgtgtaagc cgaaagcttc 3901 attggaggag acggaagtgc tgtttgtttt catagggtat gaccggaggg ttcgtacgca 3961 caacccatac aaactatcct ctaccctgac caatatctac accggatcgc acctgcatga 4021 ggccggatgt gccccttcct accacgtggt acgtggagac attgccaacg ccgaggaggg 4081 ggttattgtc aacgcagcaa atagcagagg gcagccgggt gggggtgtat gtggggctct 4141 ctacaaacgc ttcccggaga gtttcgacat gcaacccatc gaggtgggta aatcaaggtt 4201 agtaaaaggg gcagcgaagc acatcattca tgctgtgggg ccgaatttta acaaggtatc 4261 agagctggaa ggggacaaac aattggccga agcttatgaa tctgtcgcta agattattaa 4321 tgacaatcat taccggacag tggcaatacc gcttttgtct actggcattt ttgccgggaa 4381 caaggaccga cttatgcagt ctctgaatca tttgctcacg gcgttagata ctaccgacgc 4441 ggacgtggct atatattgta gagacaagaa gtgggaggcc accctgaaag aagttatagc 4501 aaggagagag gctgttgaag agatatgtat ctcggaagat gcgtctattg cggaaccgga 4561 cgctgaattg gtcagggtac atcccaagag ctccctggcg gggcgcagag gctttagtac 4621 gaccgatggg aagacattct cataccttga ggggactaaa tttcatcagg cggctaagga 4681 tgtggctgag atcaacgcga tgtggcctaa tgcaactgag gcgaatgaac agatctgcct 4741 gtacatcttg ggagagagca tgagcagcat tcgttcaaag tgtccagttg aagaatcaga 4801 agcttcgaca ccacccagta cattgccatg cctatgcatc cattccatga cacccgaacg 4861 agttcacaga cttaaggcct cacgtcctga gcagataacg gtgtgctcgt ctttctcgtt 4921 gcccaagtac aggatcaccg gagtgcaaaa aattcaatgc tctcatccta tactcttcct 4981 cccgaaagtg cctgagtaca ttcatccgcg caggtatctt ccccaaacgg agaaccctgt 5041 ggatccacct gaacttccgg agccttcagc cgtcgccaca cctacagcag acggtcagac 5101 agaggaggcc atgagcataa tgtcggaaac gactgttgcc tctcttgtac ctaatgagat 5161 tgagccatca accgccgcca cacctgcagc agatagtcag acagaggaga ccatgagcat 5221 aatgtcggaa acgactgttg cctcttttgt acctaatgag attgtggtcg aagcgcaagt 5281 acactcagac cttatgcgct tctcctctag ctggtcaata ccgcaagcat ctgacttcga 5341 tgtagacagc gtgtcagtcc agggcaccgt ggatatattc aacagtgcgc tgcaggagga 5401 tgcgagtagt gtagcatcgt cgtactgctc gggaataaaa cctgtccctg caccgcgcac 5461 tgtttttact caatcaccaa aacctcgcag acggcgccgt cgatctaaag ccagcacgag 5521 ttctacggcc cctctggtga ccacatcccg tgctacatca cgatcaagcc ttgtctcaaa 5581 tccacctggg gaaaaggtga ccacatcccg tgccacatca cgatcaagcc ttgtctcaaa 5641 tccacctggg gaaaagaggg ttattactag agaggagttc gaggcctttg ttgcgcaaca 5701 gcaatgacgg tacgaagcgg gtgcgtacat tttttcctct gataccggtc aggggcattt 5761 acaacaaaag tcagtgaggc agacgacgct gtccgaggta gtacttgaac ggacagagtt 5821 agagcattcg tacgccccgc gcctcgacct agagaaagaa gagatactac gtaaaaaatt 5881 acaattgaac ccgacaccag ctaataggag cagatatcaa tccagaagag tggagaacat 5941 gaaagcggtg actaccaaaa gactcctcgg tggtttgggg agctacctga aaacagaagg 6001 aaaggttgaa tgctaccgta cactgtatcc tgttcccgag tattctgcaa gcgtcaacca 6061 agtcttttct agtccgcgcg tggctattga agcatgtaat gctgtattga aagagaattt 6121 tccaacagtg acttcatact gcataacacc cgagtatgac gcctacatgg acatggttga 6181 tggagcctcc tgctgtttag acactgctag cttttgccca gcaaagttgc gcagctttcc 6241 gaaacgacac gcctatctgg agccaactat tcgttctgca gtaccatcag ccatacagaa 6301 taccttgcag aatgtgctgg cggcggccac aaaaagaaac tgtaacgtta cccagatgag 6361 agagttacct gttttggatt ccgctgcttt taacgtggag tgttttaaaa aatatgcatg 6421 taataatgaa tattgggaaa cgtacaggga acatcctatt aggctaaccg aggagaatgt 6481 aaccaattac attactaaat tgaagggccc gaaagcagcc gctttgtatg caaaaactca 6541 taaccttagc atgttgcagg atattccaat ggataggttc gttatggact tgaaaagaga 6601 tgtcaaggtg acgcctggta ctaaacatac ggaggagagg ccgaaggtgc aggtcatcca 6661 ggcggcggac ccattggcta cggcttacct gtgcggaatc catcgagagc tagtgcgcag 6721 gctgaatgcg gtcctattgc ctaacgtcca taccttgttt gacatgtctg ctgaagactt 6781 cgacgccatt attgccgaac attttcagcc cggagacagt gtgttagaga ctgacattgc 6841 gtcgtttgat aagagcgagg atgatgcaat ggcgttgact gctctgatga tattggagga 6901 tttgggcgta gatccagagc tgctcacact aatagaggct gcatttgggg agatatcatc 6961 catccatctc cccacgaaaa ccaagtttaa atttggagca atgatgaagt ctggtatgtt 7021 tcttactttg tttattaaca cggttatcaa cattgtcata gccagcagag ttctacgtga 7081 acgtctgacc aactcaccct gtgctgcatt tattggtgac gacaacatcg tcaagggtat 7141 caagtcagat aagctgatgg ccgatagatg tgctacctgg ctgaacatgg aagtcaagat 7201 catcgatgcc gtagtaggaa caaaagcccc ttatttttgt ggaggcttca tcttgtgtga 7261 cacagtaaca ggtacagcat gtcgggtggc tgaccctctg aagagattgt tcaaactagg 7321 gaagccgctc gcagcagacg acgaacatga cgatgatcgt cgtcgggctt tgcatgagga 7381 gtcagctcgc tggaaccgtg tgggcataca ctccgagctg tgtaaggccg tagagtcacg 7441 ctatgaaact acaggtacat ctgtaatcat aacagccatg actaccctgg cacagagtgt 7501 ctccgcattc aaacatctga gaggaaaccc tctgaccctc tacggctaac ctgaatggac 7561 tgtgacgtag tacagtcccc aaaaatgttc ccttatcaac caaccatgtt tccaatgcaa 7621 ccagcaccct ttcgcaatcc ttatgctgcg cctagaagac cgtggttccc gaggaccgac 7681 cccttcttag ctatgcaggt gcaggagttg gcccggtcga tggcgaacct gacgtttaaa 7741 cagcgtcggg gcgccatgcc aggcgggccc cctgcaaaaa agaagcggaa ggaaccacag 7801 cagcaggtta ctcaggcgca ggataagaag aagaatggaa agatgaaaaa gaagaaacgt 7861 aatggagcag ccccccctaa aaataaaaat agtcccaaga agaaaacgaa caagaaaccc 7921 ggaaaaaggc agaggatggt catgaagttg gaatctgata aaacatttcc gatcatgctg 7981 gacggaaaga tcaaccgata tgcttgcgtg gtcggaggca agctattccg acctatgcat 8041 gtagaaggaa aaattgataa tgacaccctt gccgcattga agatgaagaa agcatccaaa 8101 tatgacttag agtatgctga tgtaccccag aacatgcgct cagatactct taaatataca 8161 catgagaaac cccagggata ctataactgg caccacggcg ccgtgcagta tgaaaatggg 8221 agatttacag tgccgaaagg agtaggagca aaaggagaca gcggacgccc tatattggac 8281 aaccagggca gagtagtggc tatagtgctg ggcggtgcaa acgagggatc aaggacagct 8341 ttgtccgtag tcatgtggac tgaaaaaggg gtcacagtga aatacacacc agagaattgc 8401 gagcaatggt cgctggtcac caccttgtgc ctcctcgcga acgtcacatt cccttgctcc 8461 acacccccga tttgctacga ccgagcacct gccgaaactt tgacaatgct cagtaccaac 8521 attgaaaacc cgggttatga tgaattgctt gaagccgtat tgaagtgtcc aggtaggcag 8581 aagagatcca cggaagcgct gtttaaagaa tacaagctaa cacgcccgta catggcaaag 8641 tgcgtccgat gcgcagtggg cagctgtcac agcccaattg ccatagaagc agtgcggagt 8701 gatgggcacg acggctacat taggttacag acatcatctc agtacggttt ggaccccgca 8761 ggtaatgtga agagcagagt tatgaggtac aatctgtatg gcaagatcgt agagattcca 8821 ctacatgaag tctcgttgca tacatcgcgg ccgtgtcaca tcatagacgg ccatggatac 8881 ttcctcttag cacgctgccc ggaaggagac tctattacta tggagtttag aaaggattca 8941 gtcacccact cttgctctgt gccgtatgaa gtgaaattta caccagtggg cagagaactg 9001 tacagtcatc cgcccgagca tggagcggaa cacccatgcc gtgtgtacgc tcacgatgcc 9061 cagcagaagg atgcgtatgt agagatgcac ctacccggtt ctgaagtaga cagttcgtta 9121 ctctccatga gtggtagcgc tgtccgggta acaccaccgt ctgggcagaa cgtgcttgta 9181 gagtgtaatt gcggctcggc agtatctgaa accatcagca ccgcaaagac atacaatcag 9241 tgcactaaga cctcccagtg ccgtgcgtat cgcttacaga atgacaagtg ggtgtacaat 9301 tcggataaac ttccaaaagc atcaggagaa accttgaaag gaaaattgca cgtgccctac 9361 cttctgtctg atgcgaagtg cactgtgcca ctggcaccgg aaccaatagt gacctttggt 9421 ttccggtcca tttccttaaa attacaccca cgcaatccaa cctatctgac tactcgccaa 9481 ctagatggcg agccaaatta tacccatgaa cttatttcag aaccctcgat tagaaatttt 9541 actgtgacgg agcacggatg ggaatacgtt tggggcaacc atccgcccca acggtattgg 9601 gcacaagaaa cagctccagg taacccacac gggctgccgc atgaggtgat tattcattac 9661 tatcacaggt accctatgtc tactacactc ggattgtcga tctgcgccgc tgtggtaatc 9721 acagcaatag ccgcatccac ttggctattt tgtaagtcgc gagtatcctg tctgactcct 9781 taccgattga cgccgaatgc tcagttacca gtatgcttag cgttcttgtg ttgcgcaagg 9841 accgctaggg cagagacaac atgggaatcc ctggatcacc tctggaatca taatcaaaac 9901 atgttttgga gccagttgct cattccacta gccgcgctta ttgtgttgac ccgcctactg 9961 aagtgtctgt gttgcgtagt tcctttttta gtcctagcag gcgccgcaag cgtcggcgcc 10021 tacgagcacg caaccacgat gccgaatcag gcggggatgc catttaacac agtagttaat 10081 cgcgcagggt atgcaccatt agtcatcagc attaccccga caaaggtaag gctcattcct 10141 acattgaact tggagtatgt tacatgccac tacaagacag ggcttgactc accttccatc 10201 aagtgctgcg gaacgcagga atgtccaaag gtaaacagac ccgatgagca atgtaaggta 10261 tttaccggtg tgtacccttt tatgtggggc ggcgcatact gcttctgtga ctctgaaaac 10321 acccaactca gccgcgcgta tgtgacaaaa tctgacgact gctcagctga tcacgctcta 10381 gcttacaaag cccacaccgc ctcagttcag gcgttcttga atatcactgt gggagagcag 10441 tcaacaacag cagtggtgta cgtgaatggt gaaacgccta ttagttttaa tggcatcaga 10501 ttggtcgcag gtcctttatc tactgcttgg acgccgtttg acaggaaggt ggtgcagtac 10561 gctggagaga tatataatta tgatttccca gagtacggag caggacacgc cggagcattc 10621 ggagatcttc aagccagaac gacaaccagc aatgacctgt atgccaacac caatttggtg 10681 ttgcaaagac ccaccgccgg aaccattcac gtgccttaca cgcaggcacc atcaggcttt 10741 gaacagtgga agaaagacaa gcctccatca ctgaagtata cagcaccatt tggatgtgag 10801 atctatgtga atcctgtcag agctgaaaat tgtgccgtgg gatctatacc attagccttc 10861 gacatacccg acgccttgtt taccagggtg tctgaaacac cgacactgtc gaacgcagag 10921 tgctccttga atgagtgcgt atactcatct gattttggcg ggatcgccac tgtcaagtac 10981 acagcgagta aatcaggcaa atgtgcagta catattccct caggcacggc gactctgaaa 11041 gaatcactag tggatgtggt agaacaagga tccctgaccc ttcatttttc aactgccaat 11101 atacatccgg aatttaaact tcagatatgt acaacgcatc ttacatgcaa aggcgattgt 11161 catccgccta aagaccatat agtaacacac ccacagctcc acgcccagac atttacagcc 11221 gcggtctcta agaccgcatg gacgtggttg acttcactcc tgggaggttc agccgtaatt 11281 attataatcg gccttgtgtt agcaactgtt gttgctatgt acgtgctgac caaccagaga 11341 cacaattaaa ccgcagtagc aattggcaag ctatataaaa gaattataaa gcgataattg 11401 gcaaatcgca tgtaaatccc agcagcaatt ggcacgctgc atatataatt tacttggcgg 11461 caattggcaa gccgcccata acaaattttt attttctttt ccaataattg gattttgttt 11521 ttaatatttc //