LOCUS MK757448 15675 bp DNA linear PHG 04-MAY-2019 DEFINITION Arthrobacter phage Idaho, complete genome. ACCESSION MK757448 VERSION MK757448.1 KEYWORDS . SOURCE Arthrobacter phage Idaho ORGANISM Arthrobacter phage Idaho Viruses; Caudovirales; Siphoviridae. REFERENCE 1 (bases 1 to 15675) AUTHORS Oduselu,T.J., Taiwo,A.E., Ayodele,I.E., Oyebamiji,T.O., Atoyebi,A.N., Omolola,C.M., Lazarus,F.U., Jose,L.A., Akinlolu,E.A., Ojebola,B.M., Olatinwo,S.O., Raifu,M.K., Adebiyi,I., Ogunleye,V.O., Faleye,T.O.C., Bakarey,A.S., Adewumi,O.M., Anetor,J.I., Ademowo,O.G., Garlena,R.A., Russell,D.A., Pope,W.H., Jacobs-Sera,D. and Hatfull,G.F. TITLE Direct Submission JOURNAL Submitted (04-APR-2019) Biological Sciences, University of Pittsburgh, 367 Crawford Hall, Pittsburgh, PA 15260, USA COMMENT Phage Isolation, DNA preparation and sequencing by Illumina to 6682x coverage perfomed at Smith College, Northampton, MA Annotation analysis performed at University of Ibadan, Ibandan, Nigeria. Assembly performed at Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, PA with Newbler and Consed software as of Jan, 2018. Supported by Science Education Alliance, Howard Hughes Medical Institute, Chevy Chase, MD. ##Assembly-Data-START## Assembly Method :: Newbler and Consed v. Jan-2019 Coverage :: 6682x Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..15675 /organism="Arthrobacter phage Idaho" /mol_type="genomic DNA" /isolation_source="soil" /db_xref="taxon:2565509" /lab_host="Arthrobacter sp. ATCC 21022" /country="USA: Northampton, MA" /lat_lon="42.33 N 72.64 W" /collection_date="01-Oct-2017" /collected_by="Madison Biasin and Jess Matsuoka" /identified_by="Madison Biasin and Jess Matsuoka" gene 119..295 /gene="1" /locus_tag="SEA_IDAHO_1" CDS 119..295 /gene="1" /locus_tag="SEA_IDAHO_1" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QCG78266.1" /translation="MLLGEIARDLPPLHRLDLLEVCQLLGLDPDDTDLIRIQPDGVEW RNRRGDLSHRRVVD" gene 292..474 /gene="2" /locus_tag="SEA_IDAHO_2" CDS 292..474 /gene="2" /locus_tag="SEA_IDAHO_2" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QCG78267.1" /translation="MIHGSLAPWPDCVCGSKSWKQCQDLLSAQAAEEAAQARQARAAL VDQERIDRLFWRGFDT" gene 471..2039 /gene="3" /locus_tag="SEA_IDAHO_3" CDS 471..2039 /gene="3" /locus_tag="SEA_IDAHO_3" /codon_start=1 /transl_table=11 /product="terminase" /protein_id="QCG78268.1" /translation="MTITADADELAAPELHQDGLPLDLSSIDWDAAKDRMIDTWYQRS GVLYAAQGLVSPIPEGTDIANAVKGSRMLGLELTPQGCEVAGVLEAKNAFGLPLYDDV TVQIPRRATKTTSIQTVLLGRCENRPGYRIIQTAQDGTRASMVFMDMVRTMERVTPDP EDRNWKVFKSTGREYLEWDNGSRWWVGPPKSSTYRGLAADVLWFDESGELSPEESDDL EAGALPVMDTRPDGQVIKSGTPGLVRAGLFWKSLAVAEENPAQAGVVNYAAKESDVVD LDEDAAMALLWKHHPGMACGLTTEAKMRKRFQVMDLAQFIREYLCVWPPDTTITALDM VKFAAGECNPVGLPSNVPYAFGFNVAQGGVAAAVAVAWYDGEDLHAQIMEYRLKADWV PDDLARALKAHLGTDIGYDSIGDNVATAQAVARKPRVKTTNLRALTMKEVAAGTAAIA QANDGGRFYWGTSPALLAAAKGASWRDSGGSRLFRRIQGQDISALLAVVHAVAAVATK KKRRAGGGGYAPQG" gene 2408..3580 /gene="4" /locus_tag="SEA_IDAHO_4" CDS 2408..3580 /gene="4" /locus_tag="SEA_IDAHO_4" /codon_start=1 /transl_table=11 /product="portal protein" /protein_id="QCG78269.1" /translation="MPNLTDLLTLTKRAIMGSPSPLEFGSSTSGGYTWADGMALSPIL PPSIAGGAVSVKDALRCPPVARGVGLYAAAMLQMDWEAGDATKAWLNKGSGIVSPRLR NVRTMTDLLLANRALWITSGTGYDRQEAVHLHAHLWSVDELGTISLSGVLPDANGDYV MGGRVVTNVANLIYFPGAMPLGFLENAQDSLSYYLDILATIKSRAAAPMAVMELKILD SYDGPEPGDDDYDEDEDPIRQTQAAYVAARRMPDGAVTVTPKDVDLIVHQVEDDGHML IEARAALRVDVANHLNMPASMLDGETGSSSDYANTLQKRNEFEDLSLALFTDPIAARL SMDDVTPPGELVDFKPLDMAPVAVGNTGDAVGNLQAGQLPRDNKNREITRDPENAL" gene 3606..5234 /gene="5" /locus_tag="SEA_IDAHO_5" CDS 3606..5234 /gene="5" /locus_tag="SEA_IDAHO_5" /codon_start=1 /transl_table=11 /product="major capsid and protease fusion protein" /protein_id="QCG78270.1" /translation="MTTIQILGELATASAAESGPLRYRVVPFGETSAVMASGHQFAVD PGVLTLPADPTTAHVNEEHDRTRPVGWFAGLEETQDGIYGDVELADTVAGRDARVLAA KGLRRGISVEIENPVIRAGRLVSGVLAAAGLVVNPSFANAALASALPVAPDMGDLAED LDKAAQAIKAAQDKLSAPKEPAADSAPDTATASTDKKDTNVTVTANAPSAAAAAAAIA GAASGTASTVVNAVAAQGGALRALGAAMAAYASPISNPTARAAAMDTVIQADVFDATS VPEYVGELWKGRRYAGRYLDLVSEVPMVSQSVIGWRWVAGKAPQVARWTPSTGATMND IPTNEVQAESKTYKGFRIAGGHQVDRIHVDLPSGEWWQSYNEEAADDYARKLDAEVIA HVTDPANLTALVLPAGTSTQKRLLRGIGQAIEFADPDHILIGWDIFEEMADTKMLDQK AVSPERFAQSGGSFHISYLDIPVKVAPYSLTSVKTKIQIGSKNANKLHSLPGGPIRVD AEEIQKGTIQHGLFGYHILRPTDTRGSIDVQLAP" gene 5238..5582 /gene="6" /locus_tag="SEA_IDAHO_6" CDS 5238..5582 /gene="6" /locus_tag="SEA_IDAHO_6" /codon_start=1 /transl_table=11 /product="head to tail adaptor" /protein_id="QCG78271.1" /translation="MPTSLDSTIGWLTVQEVLDLWPDAPGEDLDNLAELANLMGTAHE FLQGKGPKIDPPPESYKSAQILYMKHLWARGTAGNGDTMGPDGYQVSTFPMVLEAIGL MKLHRRRVRGLR" gene 5579..5986 /gene="7" /locus_tag="SEA_IDAHO_7" CDS 5579..5986 /gene="7" /locus_tag="SEA_IDAHO_7" /codon_start=1 /transl_table=11 /product="tail terminator" /protein_id="QCG78272.1" /translation="MTALSLSAAEQLRDKLQAALDAKKWKWVAAPEEPANVNRQRFGA VWRTRLAAASNGRLDTELVINIYGAKAAPGEATERELDDALDQVLLVLQAQPGIKFQE ATRVVFKEVFAGWDVRFSVNHNNFYAEQVRKNG" gene 5993..6406 /gene="8" /locus_tag="SEA_IDAHO_8" CDS 5993..6406 /gene="8" /locus_tag="SEA_IDAHO_8" /codon_start=1 /transl_table=11 /product="major tail protein" /protein_id="QCG78273.1" /translation="MAHTQLEIKNATVKAAPGASALVDYADAIDNVKLQTEYTTGTFK GVSGKRAITAGSINDKIVINLGDSLKTGEFWSFLRANHGVTGKVEFCPAGGTTPKIAA DVMFFAPGSVGGAEGANSSSVTLECLTPATITAAP" gene 6410..6940 /gene="9" /locus_tag="SEA_IDAHO_9" CDS 6410..6940 /gene="9" /locus_tag="SEA_IDAHO_9" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QCG78274.1" /translation="MGGSLLTPSAKRVDELRAAALALRLLPKGIRNDINKNRRAVLNP MWRDAVNAKAVTTMDKLVLAKGARVTPGNPARAMAATSKRPLSGGLVPDDRDTAVAFE FGSPERQKEVEYARRSPKGGRHQVKRHTKRQLPMLARKGRVIYAAWKATAPRIIALDT QTIARLIYRAYEGKSN" gene 6937..8763 /gene="10" /locus_tag="SEA_IDAHO_10" CDS 6937..8763 /gene="10" /locus_tag="SEA_IDAHO_10" /codon_start=1 /transl_table=11 /product="tape measure protein" /protein_id="QCG78275.1" /translation="MTIKFDFVSDVSGVTRGTDDIAERFDAVAESLVDLAKGGDAAGD KLEASLDSAGAAAQAMAREGAQAGDKLEASLDQAGQAAKGLGRDGAAAGDKLDAGLDD GSKAADKLERKLEDALDAAQDLGKSRGLDKLEGDLDAAGKSADTFEDKARKAFKSVGD DARKSGDVVGSATKKGTKEASEAAEEFKDEAKSNLSEVASSFSGSMDSAVDLVQGTLG GLAANLGSAGLIGTAVAAVGIGLAKAFAEGEAERINNMGEAVTALATELRGLDGDLSK VNFDKYMEEWGLAIQDTREWWELWQDHAETGLEDISKKAKDAGVDWKEAFRGTKGSME DSQKALDVVNKKLDEVTKSAEIYVDAMSGMTTMDPDSQRQIDALKELKKGYEDNISVQ QRAEEANRLLEAAGVKTTDQIEAEKDAVDKANDALEEHASRLAEAAGAAMSADQAEMD YGEALRTAEADIRANGKSLDINTASGAANKQTLIDLASKANALIQAQIDQGASTADVT ARSEAARASFINQAKAAGATAEEAETLANRYGLIPKNVDTMVKAHNVAQTKTEIDGVA APRTATVNVVKGTENVTSWIDSLRNSTIPVMIRRAPGSDVIP" gene 8822..10219 /gene="11" /locus_tag="SEA_IDAHO_11" CDS 8822..10219 /gene="11" /locus_tag="SEA_IDAHO_11" /codon_start=1 /transl_table=11 /product="minor tail protein" /protein_id="QCG78276.1" /translation="MGNTSAELLPVSGAVRLSINTLSGIRRITRADANGINDVRALPG TYGKPTASKPGRTNLIMNPSFEVDNAGWSLDGVTTVLQSAFPQMTQAGTKMIQCSADG TRAAPGISIISASYRPQVVEGQWLGIQVFMATENANYQTRLQIGWQDATGTTLSYVAT PWKATPFYAGDKPRLVLQAPAGTASIKVYLQFRNGDDSVSFVPAGKRMWADGCMAFVA ATEAAATAALDAGYFDGATANDDGRATSWAGTAHLSQSLELVPESPLVITDYEPASGP LRYDVEPMSGNLETLDVSGFELGGPWLFTPIIPGYSRPLVLVQELASNYTSNTTVHDG LLGRDDPIAVMRPLGMRRGTMKIFAGTIAQALELLTPLRQATVMMLRQPDHAGLDMYF AAESAELIHYDQDGPATLWGVSITYVEVKRPEGDLAGALGWTYLDLLSATPRYSDQRR TFATYADMRLNKRKP" gene 10216..11331 /gene="12" /locus_tag="SEA_IDAHO_12" CDS 10216..11331 /gene="12" /locus_tag="SEA_IDAHO_12" /codon_start=1 /transl_table=11 /product="minor tail protein" /protein_id="QCG78277.1" /translation="MTDLTYPYDARAFELVKQSHRQDGASTLILDGEELPVVLRDVQV TMDESWSPFVQFQAEGVAPEDAALLARIDPRAKALVDVRAGYVYEDGTADIQRLALVQ LQSRRAILPSNSMPLVGHGAELRAHESKWIKATTTKTFTGVMEALQFLVNYAQGNKTA AWVATVDPGYRPDLVSAVVLEQGADVWRVMNEIALSAELRLWADENGVWNLAPKVTLA GVTAAFLVQGPNTTVKEVEDVLSRDGWYNAAVLKYTWRDAGGVDQVVYGTYAPAAPSG LDQGAGCCTYSAERPGPISQYQANLNAKSVVSNLSTRGDSYVVKSVAMYWLRPGMTVQ VDLANGTSARHIIRRIAFQVSTGTMTLTTREPNNTEE" gene 11337..11855 /gene="13" /locus_tag="SEA_IDAHO_13" CDS 11337..11855 /gene="13" /locus_tag="SEA_IDAHO_13" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QCG78278.1" /translation="MPTTTKRAYRYPSQGAEPDTAGDIQRLAEDVSNDVDAINAGLTA VRTTTSKTFALGSLYKAWNTTIYEAPGLVRNDLEWVAMHGVLGMATGQASIVFNGGTQ YDIATLDAIDRPAKDRIFTPKVGNNMAGAWTVYVRSTGKVQLEVSTTQTVTNANFFVS FDQMGFQALAAV" gene 11855..12808 /gene="14" /locus_tag="SEA_IDAHO_14" CDS 11855..12808 /gene="14" /locus_tag="SEA_IDAHO_14" /codon_start=1 /transl_table=11 /product="lysin A" /protein_id="QCG78279.1" /translation="MAAGTIRLVHPSKAPVSQRCLAIQLDGLKHTGQDYYYSYGGEIF PDAYAMDAGTVIYSGDSRALGWPNAWYLNPDFDRNDDRDDSAGNIVVIGHWYGVTIYG HLESWDVVAGQDVAQGQRIGTVGSTGRSSGKHLHAELLLAPFNFNTDTYGRTDINLFI NMEGTDMTPHELLIHPAYTGGPQVSEVLKMMVQVYQGMFFGGPSTPYKASLFNLVDDV ARRSALATANFPVTRGDKTVRWVQDTANGTSGLEVLLTSALPNLAEQLKLDPETIRSA FLEAIGNPSVTIDFHDAGPATLATEPPAALMAPAAEVAAGE" gene 12801..13004 /gene="15" /locus_tag="SEA_IDAHO_15" CDS 12801..13004 /gene="15" /locus_tag="SEA_IDAHO_15" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QCG78280.1" /translation="MNEKRSIGPVTAASTVGAAAACLAIGLGNRLGWDVTVDEAWSLV IVGTALGGFLVKPKAPTPPGRHE" gene 12997..13269 /gene="16" /locus_tag="SEA_IDAHO_16" CDS 12997..13269 /gene="16" /locus_tag="SEA_IDAHO_16" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QCG78281.1" /translation="MSDDISTGELGRRMDALGASVNTGFSELRTAVEARPDWADVRRV EKGLLDKLEALAARVTKAEAWGTWANRLALGAIGAALLAAVVIKPI" gene 13517..13774 /gene="17" /locus_tag="SEA_IDAHO_17" CDS 13517..13774 /gene="17" /locus_tag="SEA_IDAHO_17" /codon_start=1 /transl_table=11 /product="DNA binding protein" /protein_id="QCG78282.1" /translation="MPGRYKRPGNQAQMIVPSDLIGAIETANMLGIDRSTLTRRIQAG TVVPLAQLDGPGGAYVFDKSEIAALVQEQAKNPPTRGIPKQ" gene 13771..13965 /gene="18" /locus_tag="SEA_IDAHO_18" CDS 13771..13965 /gene="18" /locus_tag="SEA_IDAHO_18" /codon_start=1 /transl_table=11 /product="DNA binding protein" /protein_id="QCG78286.1" /translation="MRVRPVFVSITSAATLLGVSPRTVKRYITDGHLDAGELPHGQVR LSRAQVIGLVRTSTERTPQL" gene 13962..14840 /gene="19" /locus_tag="SEA_IDAHO_19" CDS 13962..14840 /gene="19" /locus_tag="SEA_IDAHO_19" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QCG78283.1" /translation="MSQGKTFTVRRGQTAVPNRTIYDGFIGLGVTDGVANRLSVQAIG VLVLALSRPDDAPAGYRAYLGRGMGRDALLKALRELGAAGLRWQFKRTTAAGTMVTDT IISESPLTEDEAMAEWTAFVAKLDLAERAAAKEAAKAHAIEQERLALERAQADARAAE QAFRALAERLGVQAAQDAQASAFDGAPISGARLAGQAVPPCDGLPAHGEPAHVSLGDT QVSKETSSFIDREIQANEKAGAVELPSRGGAGGRDDAAQGPGREAVRDLLAERRNRRE LKRKSLRSGLDQEAGA" gene 14847..14954 /gene="20" /locus_tag="SEA_IDAHO_20" CDS 14847..14954 /gene="20" /locus_tag="SEA_IDAHO_20" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QCG78287.1" /translation="MDSHPWMLPTGTEWWQGRPYSPPSGTGLDDDRLGA" gene 14970..15386 /gene="21" /locus_tag="SEA_IDAHO_21" CDS 14970..15386 /gene="21" /locus_tag="SEA_IDAHO_21" /codon_start=1 /transl_table=11 /product="HNH endonuclease" /protein_id="QCG78284.1" /translation="MPLSVMKMSWNGMAKRKPQQGQLFHMEPLVIPGTDQGTRKKWGG RKVAKARDTCRAMLPAACWRGCGRVITADWPEKDWHAGHVDGRAEGGADNVTNYLPEC RWCNLREGGRLGAAITNGKRVAVTTDAIRERTLKWY" gene 15377..15556 /gene="22" /locus_tag="SEA_IDAHO_22" CDS 15377..15556 /gene="22" /locus_tag="SEA_IDAHO_22" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QCG78285.1" /translation="MVLKQEALDNLVVLESGTELTATKAGLVLSTKLPKGAGRREIGA HLRAVASSIEGGPEL" ORIGIN 1 gcttccgttg cgcgcgtggg atgcgttgtt gaggagtcgc gagaattgcg ggaccttcca 61 ccagacccat tctgggcggc ccagtgggac gcggccacgc gagcatggga gacggccaat 121 gctgctaggt gaaattgcgc gggaccttcc gccgctgcac cggctggacc tgttggaggt 181 ttgtcaactc ctgggcctgg accctgacga cacagaccta atccgcatcc agccggacgg 241 cgtggaatgg cggaatcggc gcggcgatct tagccaccgt cgcgtggtgg attgatccac 301 ggcagcctgg ccccctggcc ggattgtgtt tgcggcagca agtcctggaa gcaatgccag 361 gacttgctgt ccgcccaggc ggccgaggaa gccgcccagg cccgccaggc gcgggccgcc 421 ctggtggacc aggaacgaat tgacagactt ttttggagag gttttgacac atgactatta 481 ccgctgacgc cgacgagctg gcggcccccg agctgcacca ggacggccta ccgctggacc 541 tgtcgtccat cgactgggac gccgccaaag accggatgat tgatacctgg taccagcgtt 601 ccggcgtcct gtatgccgcc cagggccttg tctctcccat ccccgagggg acggacatag 661 ccaacgccgt caaggggtcc cgcatgttgg gcctggaact gacgccccag ggctgcgagg 721 tcgccggggt cctggaagcc aagaacgctt ttggccttcc gctgtacgac gacgtaacgg 781 tacagattcc gcgccgtgcc accaagacga cttccatcca gacggtgctg ctgggccgct 841 gcgagaaccg ccctggatac cggatcattc agacggccca ggacggcacc agggcgtcca 901 tggtgttcat ggacatggta cggaccatgg agcgcgtgac gccggacccc gaggaccgca 961 actggaaagt gttcaagtcc accggccgcg agtacctgga atgggacaac gggtcccgct 1021 ggtgggtagg cccgcccaaa tcgtccacct atcgcggcct tgctgcggac gtgctttggt 1081 ttgacgagtc cggcgagctg tcccccgagg aatcggacga cctggaagcc ggggcgctgc 1141 ccgtcatgga caccaggccg gacggccagg taatcaagtc cggcacgccg ggcctggtcc 1201 gtgcgggcct gttctggaag tccctggctg tcgctgagga aaacccggcc caggcgggcg 1261 tggtcaacta cgccgccaag gaatcggacg tggtggacct ggacgaggac gcggccatgg 1321 cgctgctgtg gaagcatcac cctggaatgg cctgtggctt gaccacggaa gccaaaatgc 1381 gcaagcggtt ccaggtaatg gacctggccc agttcatccg tgagtacctg tgtgtatggc 1441 ccccagacac caccattacc gccctggata tggtcaagtt tgcggccggt gaatgcaacc 1501 ctgtggggct gccgtccaac gtcccttatg cgttcggctt caacgtggcc caggggggcg 1561 tggctgcggc cgtggccgtc gcctggtacg acggtgagga ccttcacgcc cagatcatgg 1621 aataccgctt gaaggcggac tgggtgccgg acgacctggc gcgggccttg aaggcgcacc 1681 tgggaacgga cattggctac gactccattg gcgacaacgt ggcgacggcc caggccgtgg 1741 ccaggaagcc gcgagtcaag accaccaacc ttcgggcctt gaccatgaag gaagtggcag 1801 ccgggacggc cgccattgcc caggcgaacg acggcggacg gttctactgg ggaacgtccc 1861 cggcgctgct ggcagcggcc aagggcgcga gctggcggga cagtggcggg tccaggttgt 1921 tccgccgcat ccagggccag gacattagcg ccttgctggc cgtcgtccat gccgttgcgg 1981 ccgtggccac caagaagaaa cggcgagctg ggggcggtgg atacgccccc cagggctaaa 2041 aaaacaggac cccctgggcg gccagctagc ggccccaggg cggcctggcc ccccttccca 2101 ggcacagcca taccagggaa gcgctgaaaa tggcttacaa gagttttccc aggtcagagc 2161 cctgtggata accgtgtcgt ctcaccatac ggacgccccg tctcatgact gggcgtccgt 2221 aacattttgg gggaagcgcg taattttcta ggtctagcac gcttgtcaag acctatacta 2281 cggccccaca cattccagct tgttaaaact ggacacgact ggacacgact ggacagccaa 2341 aaactggccc gccaggaacg ccgaaaacgc cccttttggc cctggaaacg tcgttaatgt 2401 ttgagccgtg ccgaacctta cagaccttct caccctgacc aagcgggcca ttatgggatc 2461 ccccagcccg ctggaatttg ggtccagtac gtccggtgga tacacctggg cggacggcat 2521 ggcactgtct cccatccttc cgccgtcaat cgctggcggg gccgtatccg tcaaggacgc 2581 cctacgctgc ccccctgtcg ccaggggcgt gggcctgtac gccgccgcca tgcttcaaat 2641 ggactgggaa gccggggacg ccactaaagc ctggctcaac aagggcagcg gcattgtctc 2701 ccccaggctg cggaacgtcc gcaccatgac ggacctgctg ttggccaacc gcgccctgtg 2761 gatcacgtcc ggcactggct acgaccgcca ggaagccgta caccttcacg cacatctgtg 2821 gagcgtggac gagctgggca ccattagcct gtccggcgtc ctgcctgacg ctaacggcga 2881 ttacgtcatg ggcggccgcg tggtcaccaa cgtggcgaac cttatctatt tccctggtgc 2941 catgccgctg ggcttcctgg aaaacgccca ggactcgctg tcctattacc tggacattct 3001 ggccactatc aagtccaggg ccgccgcccc catggccgtg atggaactca agattctgga 3061 cagctacgac gggccggaac ctggggacga cgactacgac gaggacgagg accccattag 3121 gcagacccag gccgcctacg tggccgccag gcgtatgccg gacggggccg tgactgtcac 3181 ccccaaggac gtggacttga ttgtccacca ggtagaggac gacggccata tgttgattga 3241 ggcccgcgcc gccctgcgcg tggacgtggc caaccacctg aatatgcccg cgtccatgct 3301 ggacggcgag acaggcagta gctcggatta cgccaacact ctgcaaaaac gaaacgagtt 3361 tgaggacctg tccttggcct tgttcacaga cccgatagcg gcccgcctgt ccatggacga 3421 cgtgacgccc cccggcgagc tggtggactt caagccgctg gatatggccc ctgtggccgt 3481 tgggaatacc ggcgacgctg taggaaacct acaggccggc cagctgccca gggacaacaa 3541 aaaccgcgag attacgcggg accccgaaaa cgccctttag cgacaaccta caaagacggg 3601 aaaccatgac aaccattcag attctgggcg agctggccac ggcgtccgct gccgagtccg 3661 gccccctgcg ctatcgcgtg gtgccgttcg gcgagacttc cgccgtaatg gccagcggcc 3721 accagttcgc cgtggacccc ggcgtcctga cgctgcccgc tgatcccacc acggcgcacg 3781 tcaacgagga acacgacagg acccgccccg ttggctggtt cgctggcctg gaagaaaccc 3841 aggacggcat ctatggagac gtggagctgg ccgacaccgt ggccggacgg gacgcccgcg 3901 tcctggcagc caaggggctg cgccgtggca tatccgtgga gattgaaaat ccagtgatcc 3961 gtgcgggccg cctggtgtcc ggcgtcctgg ccgctgcggg cctggtagta aatccatcgt 4021 tcgccaacgc tgccctggct tccgcccttc cggtggcccc cgatatgggg gacctggccg 4081 aggacctgga caaggccgcc caggcgatca aggccgccca ggacaaactg tccgccccca 4141 aggaaccggc cgccgattcg gcccctgaca ccgctacagc atccaccgac aagaaagaca 4201 ccaacgtgac tgtaaccgcc aacgccccgt ccgctgctgc tgctgcggca gctatcgccg 4261 gggccgcatc cggcaccgct tccaccgtcg tgaacgccgt ggccgcccag ggcggcgcac 4321 ttcgcgccct gggcgctgcc atggctgcct acgcttcccc catcagcaac ccaacggccc 4381 gcgccgctgc catggatacc gtgatccagg ccgacgtatt cgacgccacg tctgtgccgg 4441 aatatgtggg cgaactgtgg aagggccgcc gctacgcggg ccgctacttg gacctggtgt 4501 ccgaggtccc catggtgtcc cagtccgtca ttggctggcg ctgggtagct ggcaaggccc 4561 cccaggtggc acgctggacc ccttcgaccg gcgcgaccat gaacgacatt cccacgaatg 4621 aagtccaggc cgagtccaag acgtacaagg gcttccgtat cgctggcggc caccaggtag 4681 accgcatcca tgtggacctg ccgtccggcg aatggtggca gtcctacaac gaggaagccg 4741 ccgacgacta cgcccgcaag ctggacgccg aggttatcgc ccacgtcacg gacccggcga 4801 acctgacggc ccttgtcctg cccgctggca cgtccaccca gaaacgactg ttgcgcggca 4861 tcggccaggc tatcgagttc gcggacccgg accacatctt gattggctgg gacatcttcg 4921 aggaaatggc cgacaccaaa atgttggacc agaaggccgt atcccctgag cgcttcgccc 4981 agtccggtgg aagcttccac atcagctacc tggacattcc ggtgaaggtg gccccgtact 5041 cgcttacgtc ggtcaagacc aaaatccaga ttggttccaa gaacgccaac aagctgcaca 5101 gcttgcctgg cggccccatc cgcgtggacg ccgaggaaat ccagaagggc accattcagc 5161 acggcctgtt cggttaccac atcctgcgcc ccacggatac gcgcggttcc atcgacgttc 5221 agttggcacc gtaacccatg cccacaagtc tggattcgac cattggttgg ctcacggtcc 5281 aggaagtcct ggacctgtgg ccggacgccc ctggcgagga cctggacaac ctggcggagc 5341 tggccaacct catgggcacc gcccacgagt tcctacaggg aaaaggcccc aagatcgacc 5401 cgccccccga gtcttacaag tccgcccaga ttctttacat gaagcatctt tgggccaggg 5461 ggacggccgg gaacggggac accatggggc cggacggtta ccaggtgtcc accttcccca 5521 tggtcctgga agcaattgga ctcatgaaac tgcaccgccg acgagttagg ggccttcgat 5581 gacagcgcta tcactgtccg ccgctgagca actgagggac aagctacagg ccgccctgga 5641 cgccaagaaa tggaagtggg tagcggcccc cgaggaaccg gcgaacgtca accggcagcg 5701 cttcggcgct gtctggcgga cccgcctggc cgctgcttcc aacggccgcc tggacactga 5761 gttggttatc aacatctacg gtgccaaggc cgcccctggg gaagcgacgg aacgcgagct 5821 ggacgacgcc ctggaccaag ttcttttggt cctacaggcc cagccgggaa tcaagttcca 5881 ggaagccacg cgagtggttt tcaaggaagt gtttgcgggc tgggacgttc gtttcagcgt 5941 caatcacaac aacttttatg ccgagcaagt acggaagaac ggataacgcc acatggcaca 6001 tacacagctt gaaatcaaga acgcgactgt caaggccgcc cctggggctt ccgccctggt 6061 ggactacgcg gacgctatcg acaacgtgaa gctccagacg gaatacacca cgggaacctt 6121 caagggcgtg tccggcaagc gggctatcac ggccgggtcc atcaacgaca agattgttat 6181 caacctgggg gactcgctca agactggcga gttttggagc ttcctgcggg ccaaccacgg 6241 cgtaacgggc aaggtggaat tttgcccggc cggtggtacc acgcccaaga ttgcggccga 6301 cgtgatgttc ttcgcccctg ggtccgtggg cggcgcggag ggcgctaact cttccagcgt 6361 gaccctggaa tgcttgaccc cggccaccat cacggcggca ccgtaagcca tgggcggcag 6421 tctgctgacc ccaagcgcca agcgcgtgga cgagctgcgg gccgccgccc tggccctgcg 6481 gctgctgccc aagggcatcc gcaacgacat aaacaagaat cgccgcgccg tcctaaatcc 6541 catgtggcgg gacgcggtga acgccaaggc cgtcaccacc atggacaagc tggtcctggc 6601 caagggtgcc agggtaacgc ctggtaaccc ggccagggct atggctgcca cgtccaagcg 6661 gcccctgtct gggggcctgg tgccggacga cagggacacg gccgtggcct ttgaatttgg 6721 atcccctgag cggcaaaagg aagtggagta cgccaggcgt tcgcccaagg gcggccgtca 6781 ccaggtgaag cgccacacca agcggcagct tcccatgctg gccaggaagg gccgcgttat 6841 ctatgcggcc tggaaggcta cggccccccg cattatcgcc ctggataccc agaccattgc 6901 ccgcctgatc tatcgagcct atgaaggtaa aagcaattga ctatcaagtt tgactttgtg 6961 tccgacgtgt cgggcgtgac caggggaacc gacgacatag cggagcggtt cgacgccgtg 7021 gcggaatccc tggtggacct ggccaagggc ggggacgcgg ccggggacaa gctggaagcg 7081 tccctggaca gcgccggggc cgctgcccag gcaatggccc gcgagggtgc ccaggccggg 7141 gacaagctgg aagcgtccct ggaccaggct ggccaggctg ccaagggcct gggccgggac 7201 ggggctgctg cgggggacaa gctggacgct ggactggacg acgggtccaa ggcagcggac 7261 aagctggaac gcaagctgga agacgccctg gacgctgccc aggaccttgg caagtccagg 7321 ggcctggaca agctggaagg cgacctggac gccgctggca agtctgccga caccttcgag 7381 gacaaggccc gcaaggcgtt caagtccgtg ggggacgacg cccgcaagtc cggcgacgtg 7441 gtgggaagcg ccaccaaaaa gggcaccaag gaagcgtccg aagctgccga ggagttcaag 7501 gatgaagcca agtccaacct gtcggaagtc gcgagctctt ttagcggttc catggattcc 7561 gcggtggacc tggtacaggg caccctgggc ggcctggcgg ccaacctggg atccgctggc 7621 ctgatcggca cggccgtggc cgctgtcgga atcggcctgg ccaaggcgtt cgcggaaggg 7681 gaagccgagc gcatcaacaa catgggggaa gccgtcacgg ccctggccac ggaactgcgc 7741 ggcctggacg gggacctgtc caaagtgaac tttgacaagt acatggagga atggggactg 7801 gcgatccagg acacccggga atggtgggag ctgtggcagg accacgcgga aactggcctg 7861 gaagatattt ccaagaaagc caaggacgcg ggcgtggact ggaaggaagc ctttaggggg 7921 accaaggggt ccatggagga ttcccagaag gccctggacg tggtgaataa aaagctggat 7981 gaagtcacca agtcagcgga aatctacgtg gacgccatgt ccggcatgac caccatggac 8041 ccggattccc agcggcaaat tgacgcccta aaggaactga aaaagggcta tgaggacaac 8101 attagtgtcc agcaacgggc cgaggaagcc aacaggctgc tggaagctgc aggcgtcaaa 8161 accacggacc agatagaggc cgagaaggac gctgtagaca aggccaacga cgccctggaa 8221 gaacatgcgt cccgcctggc ggaagccgct ggggcggcca tgagcgcgga ccaggcggaa 8281 atggattacg gggaagcgct gcggacggcg gaagcggaca taagggccaa cggcaagtcc 8341 ctggacatca acacggcgtc cggtgccgcg aacaagcaaa ccttgatcga cctggcatcc 8401 aaggccaacg ccctgataca ggcccagatt gaccagggcg catccacggc ggacgtaacg 8461 gcccgctcgg aagctgccag ggcgtccttt atcaaccagg ccaaggcggc cggtgccacg 8521 gccgaggaag ccgaaaccct ggccaaccgt tacggcctga tccccaagaa cgtggacacg 8581 atggtgaagg cccacaacgt ggcccagacc aaaacggaaa ttgacggcgt ggcggccccc 8641 aggacggcca cagtaaacgt cgtcaagggc accgaaaatg tcacgtcctg gattgactcg 8701 ctgcgcaatt ccaccattcc agtgatgatc cgacgggcgc cggggtctga cgtaatcccc 8761 tagacacttg cacagaaaaa ccaacggccg gccagggcac tagcacaaag gaaccaacac 8821 catgggaaac accagcgcgg agctgctgcc cgtatcgggc gctgtccgcc tgtccatcaa 8881 caccttgtcc ggcatccggc gcattaccag ggcggacgcc aacggcatca acgacgttag 8941 ggcgctgcct ggcacctacg gcaagccaac ggccagcaag ccggggcgaa ccaatctgat 9001 tatgaatccg tcctttgaag tggataacgc gggctggtcc ctggacggcg taacgactgt 9061 cctacagtcc gcgttcccgc agatgacaca ggccggaacc aagatgatcc agtgctctgc 9121 tgacgggacc agggccgccc ctggaatatc catcattagc gcgagctaca ggccccaggt 9181 tgtggagggc caatggctgg gcatccaggt attcatggcc acggaaaacg ccaactatca 9241 gacacgtcta cagatcggct ggcaggacgc cacaggaacc acactgtcct acgtggccac 9301 gccctggaag gccacgccgt tctacgccgg ggacaagccg cgccttgtcc tacaggcccc 9361 agccggtacg gcgtcgatca aggtctacct tcagttccgc aacggggacg actcggtgag 9421 ttttgttcct gccgggaaac gcatgtgggc ggacggatgc atggcctttg tggctgccac 9481 ggaagcggcc gccacggcgg ccctggatgc tggatacttc gacggcgcaa cggccaacga 9541 cgacggccgc gccacgtcct gggctggcac tgcgcacctg tcccagtcct tggagctggt 9601 gccggaatcg cccctggtca ttacggacta tgagccagcg tccggcccct tgcgctacga 9661 cgtggaaccc atgagcggga acctggaaac cctggacgtg tccggctttg agctgggcgg 9721 cccctggctg ttcacgccta tcatccctgg ctattcccgg cccctggtcc tggtccagga 9781 actcgcgtcc aactacacgt ccaatacgac agtccatgac gggctgttgg gccgtgatga 9841 tcccatagcc gttatgcggc ccctgggaat gcgccgggga accatgaaga ttttcgctgg 9901 cactatcgcc caggcgttgg agctgctgac gcccctacgc caggccacag tgatgatgct 9961 gcggcagccg gaccacgcgg gcctggatat gtatttcgcg gccgagtccg ccgagctgat 10021 ccattacgac caggacggcc cggccacctt gtggggcgtg tccatcacct acgtggaagt 10081 gaagcggccg gaaggggacc tggccggggc gctgggctgg acctacctgg acctgctgtc 10141 cgccacgccc cgctattcag accaacggcg cacgttcgcc acctatgcag atatgcgact 10201 caacaagagg aagccatgac ggaccttact tacccctacg acgccagggc cttcgagctg 10261 gtgaagcaga gccaccgcca ggacggcgcg tctacgctga tcctggacgg cgaggaactg 10321 cctgtggtgc tgcgggacgt gcaggtgacc atggatgaat cctggtcccc gttcgtccag 10381 ttccaggccg agggcgtggc ccccgaggac gccgccctgt tggccaggat cgaccccagg 10441 gccaaggccc tggtggacgt gcgggctggc tacgtctacg aggacggcac ggccgacatt 10501 cagcgccttg ccctggtcca gctccaatca cgccgggcga tcctcccatc caattccatg 10561 ccgctggtag gccatggggc cgagctgcgg gcgcacgaaa gcaagtggat caaggccacc 10621 accacaaaga cctttaccgg cgtcatggag gccctgcaat tcctggtcaa ttacgcccag 10681 ggaaacaaga cggccgcctg ggtggccacg gtggaccctg gctacaggcc ggacctggtg 10741 tccgccgtcg tcctggaaca aggcgcggac gtgtggcgcg tcatgaacga gattgccctg 10801 tccgccgagc tgcgcctgtg ggccgacgaa aacggcgtat ggaacctggc ccccaaggtc 10861 accctggcgg gcgtgacagc cgcgttcctg gtccaggggc ctaacaccac agtcaaggaa 10921 gtggaggacg ttctatccag ggatggctgg tacaacgccg ctgtcctgaa atacacctgg 10981 cgggacgctg ggggcgtgga ccaggtggtc tatgggactt acgccccggc cgccccgtcc 11041 ggcctggacc agggcgcggg ctgctgcacc tacagcgccg aacggcccgg ccccatcagt 11101 cagtaccagg cgaacctaaa cgccaagtcc gtggtttcca acctgtccac caggggagat 11161 tcctacgtcg tcaagtccgt ggccatgtac tggctgcggc caggaatgac ggtccaggtg 11221 gacctggcca acggcacgtc cgcccggcac atcatccggc gcattgcctt ccaggtgtcc 11281 accggcacca tgacgctgac aacccgcgaa ccaaacaaca cagaggaata agcaacatgc 11341 caacaacaac taagcgggcc taccgctacc cgtcccaggg cgctgagccg gacacggccg 11401 gggacattca gcgccttgct gaggacgtgt ccaacgacgt ggacgcgatc aacgcgggcc 11461 ttacggccgt gcggaccacc acgtccaaga cgttcgccct gggcagcctt tacaaggcgt 11521 ggaatacgac catctatgaa gcgccgggcc tggtccgtaa tgacctggaa tgggtggcta 11581 tgcatggcgt cctgggcatg gccacaggcc aggcgtccat tgttttcaat ggtggtaccc 11641 agtacgacat tgcgaccctg gacgcgatcg acaggcccgc caaggaccgc atctttacgc 11701 ccaaggtagg caacaacatg gccggggcct ggacggtcta cgtccggtca acgggcaagg 11761 tccaactgga agtaagcacc actcagacag tcactaacgc caacttcttt gtgagcttcg 11821 atcaaatggg cttccaggca ttggcggcag tctagtggcg gccggaacga ttcgcctggt 11881 ccatccgtcc aaggcccctg tatcccagcg ttgcctggct atccagttgg acggccttaa 11941 gcacactggc caggactatt actacagcta cggcggcgag atattccctg acgcttacgc 12001 catggacgcc gggactgtga tctattccgg cgattccagg gcgctgggct ggcctaatgc 12061 ctggtaccta aacccggact tcgaccgcaa cgacgacagg gacgacagcg ccggaaacat 12121 cgttgtcatt ggtcactggt acggcgtcac aatctacggc cacctggaat cctgggacgt 12181 cgtggcgggc caggacgtgg cccagggcca gcgtatcggc accgtaggca gcaccgggcg 12241 atccagcggc aagcacttgc acgctgagct gctgctggcc ccgttcaact tcaatacgga 12301 cacctatggc cgtactgaca tcaatttgtt catcaacatg gaaggcacag atatgacccc 12361 gcacgagctt ttgatccacc ccgcctacac tggcggcccc caggtctccg aggtcctgaa 12421 aatgatggtc caggtttacc agggcatgtt cttcggtggc ccgtccaccc cgtacaaggc 12481 ttccctgttc aacctggtgg acgacgtggc ccggcgttcc gccctggcta cagccaactt 12541 ccctgtgact cgcggggaca agactgtccg ctgggtccag gacacggcca acggcacgtc 12601 cggcctggaa gtgctgctta cgtccgcgct gccgaacctg gcggaacagt tgaagctgga 12661 ccccgagaca atccggtccg cgttcctgga agccattggc aacccgtccg taaccatcga 12721 cttccacgac gctggcccgg cgaccctggc cacggaaccg ccagcggccc tcatggcccc 12781 tgctgcggag gtggcagccg gtgaatgaga agcgatccat cggacccgtc acggccgcgt 12841 ccacagtggg cgctgcggcc gcctgtctcg ctatcggcct ggggaaccgc ctgggctggg 12901 acgtgacggt ggatgaagcc tggtccctgg tcattgtcgg aacggccctg ggcggcttcc 12961 tggtcaagcc caaggcccct acgccgccag ggcgtcatga gtgacgacat aagcacaggc 13021 gagctgggcc gccgcatgga cgccctgggc gcttccgtca acactgggtt tagcgagctg 13081 cgcacagccg tggaagccag gccggactgg gccgacgtgc ggcgcgtgga aaagggccta 13141 ctggacaagc tggaagccct ggcggccagg gtaaccaagg cggaagcctg ggggacctgg 13201 gccaaccgcc tggccctggg cgcgataggc gcggcgctcc tggcggccgt agtgattaaa 13261 ccaatatgac aaagatgtta tttgtaggaa acctagagcc ggccgcggcc gcctggtcaa 13321 acaaaatccg cgcgatcctg cggaagttct gttttggccc aaggggctgg gttgtaggac 13381 ccctacaaag catttagaca cgcgtcacag gctgcccaat ctgacacgcc gttaatggtc 13441 aactgtgcaa ggaaccttgc gttaatgtat ttcctgttgc aaggttcctt gcagacaaca 13501 acggaaggtt aggcaaatgc ctggacgtta caaacgccct gggaatcaag cccagatgat 13561 tgtcccgagc gacctgatag gcgctatcga gacggccaac atgctgggaa ttgaccgctc 13621 aacacttacc aggcgcatcc aagcggggac tgtggtcccc ctggcgcaac tggacggccc 13681 tgggggggcc tacgtgttcg acaagtccga gatagcggcg cttgtccagg aacaagccaa 13741 gaatccacca acgagaggta ttccaaaaca atgagagtac gtccagtgtt cgtctccata 13801 acgagcgcgg ccaccctgct gggagtctca cccaggacag tcaagcggta catcacagac 13861 ggccacctgg acgcgggaga gctgccccat gggcaggtgc gcctgtcccg cgcccaggtt 13921 attggactcg tcagaacttc aaccgaaagg acgccccagc tatgagccag ggaaagacgt 13981 ttaccgtccg gcgtggccag acggccgtac ccaatagaac catttacgac ggctttattg 14041 gccttggtgt tacggacggc gtggctaacc gcctgtccgt ccaagcgatt ggtgttcttg 14101 tccttgcgct gtccaggccg gacgacgccc cggcaggata cagggcctac ctggggcgcg 14161 gcatgggacg ggacgccctg ttgaaggcgc tgcgcgagct gggggccgct gggctgcgct 14221 ggcagttcaa gcggacgacg gcggccggga ccatggttac ggacaccatc attagcgagt 14281 ccccgctaac tgaggatgaa gccatggccg agtggacggc gttcgtggcc aagctggacc 14341 tggccgaacg tgcggccgcc aaggaagccg ccaaggccca cgcaatagag caggaacgcc 14401 tggccctgga acgggcacag gcggacgcca gggcggccga gcaagccttt agggcactgg 14461 cggaacgcct gggagtccag gccgcgcaag acgcccaggc aagcgctttt gacggcgctc 14521 cgatttccgg cgcacggttg gccggtcaag cggtcccacc gtgcgacggt ttaccggcac 14581 acggtgaacc ggcgcatgta tctctaggag atacccaagt ctctaaagag acttcgtcct 14641 ttatagacag agagatccaa gcgaacgaaa aagctggggc cgtagaactg ccatcacggg 14701 gcggggccgg gggccgggat gacgcggccc aggggccagg ccgtgaagcc gtgcgggacc 14761 tcctggccga gcgacggaac cgccgggagc taaaaaggaa gtcgcttcgc tccggccttg 14821 accaggaagc gggcgcataa gaatggatgg actctcaccc ctggatgctg cccaccggga 14881 cagaatggtg gcaaggacgg ccttacagtc cgccaagcgg aactgggctg gacgacgaca 14941 gattaggcgc ttagaacgca atctagcgat tgccgctatc cgtcatgaag atgagctgga 15001 acggcatggc aaagcgtaaa ccacaacagg gccagctatt ccacatggaa ccgctggtaa 15061 tcccagggac ggaccagggc accaggaaga agtggggcgg ccggaaggta gccaaggcgc 15121 gggacacatg ccgggccatg ctgccagcgg cttgctggcg gggctgcggc cgagtcatta 15181 cggcggactg gccggaaaag gactggcacg ccggacacgt tgacggccgg gccgagggcg 15241 gcgcggacaa cgtaaccaac tacctaccgg aatgccgctg gtgcaacctg cgggaaggcg 15301 gccgcctggg cgcggcgatc accaacggca agcgcgtggc tgtgaccacg gacgcgatac 15361 gagaacggac attgaagtgg tactgaagca ggaagcactg gacaacctgg ttgtcctgga 15421 atcaggcacc gagctgacag caaccaaggc gggcctggtc ctatcgacca agctgcccaa 15481 gggcgcgggc cgtcgtgaga taggcgcaca ccttcgggcc gtggcatcca gcattgaagg 15541 cgggccggaa ctatgactgt cgcttggata gatgaagcaa tggccgacga cgacggccgc 15601 gagttggtcc aggcgatcct ggaccgcaag acagcgagtt tttctgaggg ggccggaagc 15661 ccacgtatac cgtcc //