LOCUS KT355473 15556 bp DNA linear PHG 06-APR-2017 DEFINITION Arthrobacter phage Jessica, complete genome. ACCESSION KT355473 VERSION KT355473.1 KEYWORDS . SOURCE Arthrobacter phage Jessica ORGANISM Arthrobacter phage Jessica Viruses; dsDNA viruses, no RNA stage; Caudovirales; Siphoviridae; Decurrovirus; unclassified Decurrovirus. REFERENCE 1 (bases 1 to 15556) AUTHORS Pizzorno,M.C., Stowe,E.L., Benjamin,A.K., Blasi,C.J., Forster,G.L., Garrett,A.H., Le,A.T., Li,S., Lu,I., McGinnis,J.H., Medrano,J.E., Otero,K.L., Pinamont,W.J., Ball,S.L., Bradley,K.W., Asai,D.J., Bowman,C.A., Russell,D.A., Pope,W.H., Jacobs-Sera,D., Hendrix,R.W. and Hatfull,G.F. TITLE Direct Submission JOURNAL Submitted (30-JUL-2015) Center for Life Sciences Education, The Ohio State University, 1735 Neil Ave, Columbus, OH 43210, USA COMMENT Phage Isolation, DNA preparation, annotation analysis was performed at Bucknell University, Lewisburg, PA Sequencing by Illumina to approximately 14744x coverage was performed at Pittsburgh Bacteriophage Institute Assembly performed with Newbler and Consed software as of Jan, 2015. Supported by Science Education Alliance, Howard Hughes Medical Institute, Chevy Chase, MD. ##Assembly-Data-START## Assembly Method :: Newbler and Consed v. Jan-2015 Coverage :: 14744x Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..15556 /organism="Arthrobacter phage Jessica" /mol_type="genomic DNA" /isolation_source="soil" /db_xref="taxon:1698362" /lab_host="Arthrobacter sp. ATCC 21022" /country="USA: Lewisburg, PA" /lat_lon="40.9645309 N 76.88440704345703 W" /collection_date="01-Aug-2014" /collected_by="Jessica Medrano" /identified_by="Jessica Medrano" gene 43..348 /locus_tag="SEA_JESSICA_1" CDS 43..348 /locus_tag="SEA_JESSICA_1" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ALF00756.1" /translation="MTEYAPMLPGFEAPKTGMSKLEQRCSQHLVMLQELGLLKDQDQV MAQLVMDLAHAVALSAAAGKAAGTALAVKPLMEALDKLPKPVTEDAFAAMMKEAGLV" gene 345..560 /locus_tag="SEA_JESSICA_2" CDS 345..560 /locus_tag="SEA_JESSICA_2" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ALF00757.1" /translation="MSTNPAELTFDMNFHAFVLAVPLRTTEAGMMLGQPVIAMNQGGE AQLVMALRAIADDIEARGTDVVGKWTL" gene 591..806 /locus_tag="SEA_JESSICA_4" CDS 591..806 /locus_tag="SEA_JESSICA_4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ALM23551.1" /translation="MQVMAHSCWQRLPWSCRCSVAGDFRDHRVDGWPMQMMSMPVGWY IDVNESAGIEYPVNGDETAQRKDGQLW" gene 800..2290 /locus_tag="SEA_JESSICA_5" CDS 800..2290 /locus_tag="SEA_JESSICA_5" /note="Terminase" /codon_start=1 /transl_table=11 /product="terminase" /protein_id="ALF00760.1" /translation="MVVALADELPELLAALEQSTARYATKPTPGAPNELGQILGTAKL LGRQLMPWQIEVARVASEKRVDDPRRYRYPIVVLTVPRQSGKTTLMRTVLAQRALKCR NRKAFYTAQTGKDATARWLDLVKDIEDGPLSQFVSKRIAAGSQALTFPTGSTISPFAP TAKSLHGYTPHDVMLDEIFAHDAAAGNDLMGAIRPAQLTLPDKQLWLVSTAGTADSGF LKSWVDQGRLAVKDSGAGIAYFEWSLADGLDPYDPQNWLFHPAVGHTITLEDLADDAD SQSQGEWLRAYMNRWTSTSEAVIDVAKWDTLAGALVPVPWAQVTVAYEVAHDRSCAAI YACWKDPETGKPALKLVQQGSGAEWLAPAVAKIYVENRPKAIGADDGGPTKAVTDNLR RLPNARSGGNGVEVTTLTARDFATACVAYMGHVDDGTILHDGDPGHRAAVEAAATRPM GDSKVFSRRHSRGPIPELVAATVALRLHEQAPATAPQPAIYMGRGN" gene 2292..2495 /locus_tag="SEA_JESSICA_6" CDS 2292..2495 /locus_tag="SEA_JESSICA_6" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ALF00761.1" /translation="MIRLDKTQFSIVVLCTLCPTWRALHNDKALALAAGRRHNLTAHE GEDNTLSAARQQAYRARKAAAGA" gene 2547..3707 /locus_tag="SEA_JESSICA_7" CDS 2547..3707 /locus_tag="SEA_JESSICA_7" /note="Portal protein" /codon_start=1 /transl_table=11 /product="portal protein" /protein_id="ALF00762.1" /translation="MPLWNNPLAKPAGILARQAAASVDVLAGNVVSWEYAEPDPAPRD HFQTLTLAHLLGVEYVNIDRTAAMGIGAVAKLRKTACGFIGRMPLIAYRGHDVLARQP KIVLQPEAGRPRFVTMAWVTEALMFYGKAWFTVEERYAEDGRPARLRWVPEWKAEFNT AGQLVKAYGVDIDPADVIRVDGIDEGLLNYAQPVLREARAIDIAAGRASDNPVPSIDL HQTGGDPLTNEQIDALIERWASNRRAKNGGVSFTNQSVEAKTMGQPVEQLLIDGRNVA ALNIARAAGFPAWAVDASVNGSSITYSNSPSRTRELIDYALSPYLEAIAARFSMDDIL PAGTWCRFDYSELLRGDFAARMDAYKVAKDAEIYSTEELRAMELGRPLEVSE" gene 3704..5287 /locus_tag="SEA_JESSICA_8" CDS 3704..5287 /locus_tag="SEA_JESSICA_8" /note="capsid & capsid maturation protease" /codon_start=1 /transl_table=11 /product="capsid maturation protease" /protein_id="ALF00763.1" /translation="MKHAYLNLSAGLLTASASTRTISGEIVEYGVVGHTSLGPTIFAA GSITAPTPLSKVKMLVQHDTERSVGFLDSLEDNGTKPFAAFKVPDGAEGDDALTKAAN GTRDSFSVGVHVQEYSFDDEGNLLVHASTLKEVSLVTIPAFENALVHDVAANRKEAVM TVEEMRAQALANAQTPGNPAVALAAAAAENAPSPAEVTPSAQPATAPTRHATVAEAQA APIQVGGRRGMDLSAAANIVIEHLRNGLPATQLSAALSDVVPADDAGEGFLRPTFIGE LWQAFNDDRPLIDAFGTPGKLTGTKVYGWKWDLANRPKVGRYAGNKTELPSNPLKTVP AESDAQDFAAGWDVARKYIDLGASDFIESVFRGATADYRLQTEIWFGEQILAEATEVA GVTTVLGALSQFNVEAARIGARLSTIQFGVDAWEEFINLPEAQVPWWLKAQGSVELDG MKGRAGGVSFSANLGLGAGQILGADKRAATYYEAGSVPIRVTAQDIPRGGVDLGVFGY AGAIVHDPRAIWVSDDGLV" gene 5291..5647 /locus_tag="SEA_JESSICA_9" CDS 5291..5647 /locus_tag="SEA_JESSICA_9" /codon_start=1 /transl_table=11 /product="head-to-tail connector protein" /protein_id="ALF00764.1" /translation="MIVTVEQVRTWLGLPASDPALEDATAATNAFVERLGLPMQPKIV DGIAVLDDDGAQMFEPAADTVLGAKMLAARLYRRRNSPSGVEAITDAGTSFVARYDSD ISRYLKLDGFAAPRIG" gene 5652..6008 /locus_tag="SEA_JESSICA_10" CDS 5652..6008 /locus_tag="SEA_JESSICA_10" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ALF00765.1" /translation="MRTMGNSLKDIADMVTAAGVPAAVDPRDLNLPGAWVTPGLVSFD VLDVDTAFMTFDIYLVAPDHGAVHSLNSLGDMLAKIRPALQVGEAMPVMVNLPNHGAD ALPALLISIDAQLTED" gene 6014..6436 /locus_tag="SEA_JESSICA_11" CDS 6014..6436 /locus_tag="SEA_JESSICA_11" /codon_start=1 /transl_table=11 /product="major tail protein" /protein_id="ALF00766.1" /translation="MATDVITVGPGRFTIGSDTELTVFSGQVTSLRLVPSVDVGDSIY VLDGGEVSGDRTESWTVSGTMLQDFGATTSKTEWLFEHRGEDMPFAYAPNSARGKEIT GVLTVEAIEIGGDVKTKPTSDFEFKLVGPPAIGTVSAG" gene 6436..6813 /locus_tag="SEA_JESSICA_12" CDS 6436..6813 /locus_tag="SEA_JESSICA_12" /codon_start=1 /transl_table=11 /product="minor tail protein" /protein_id="ALF00767.1" /translation="MGTKLYAVVGGAKLRSTLRKAGADMKELSAVNRDVANIVLPVAR ATAPTTSGKLGSTVRAGATQKSAIIRVGSAKAPYGPVVHYWHKGNYTPNPWVSLAAQK TEPTWLARYHAGIERIINQVTGA" gene 6821..7171 /locus_tag="SEA_JESSICA_13" CDS 6821..7171 /locus_tag="SEA_JESSICA_13" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ALF00768.1" /translation="MAQLSAPKVIVMLESSGTDELTEYTVQTDNRDAIQWDVTRPRRS WPAFNEAPMLYMTFLAWHAMHRTGATKLSLDEFMKDAVEVKVLSAAGKAIDPTEAVAE DVLVDPTQPVAAIA" gene 7282..9279 /locus_tag="SEA_JESSICA_14" CDS 7282..9279 /locus_tag="SEA_JESSICA_14" /note="Tape measure protein" /codon_start=1 /transl_table=11 /product="tape measure protein" /protein_id="ALF00769.1" /translation="MSRTAVLAVRIVTETKEANKGIDDTVSKLDKFERGLDKAALPAA AAGTAVLAFAKKTGDMASIAQQNAGAVDSVFKGNAKTVNEFAATAADKLGLSGSAYQQ MASVIGSQLKNMGVPMDQVAGSTNDLIAKGADLAAMFGGTTSDAVDALSSLLRGERDP IEKYGVSINDAAIQAKKAELGLAGLSGEADKNATLTATMALLQKQTADATGQFAREAD SAAGAQERANAKIQDAGAKLGSVFLPAMAAAATAAGGMATWASENSTVLLVLAGIIGG VAGAILLINGALKAWRAATAAVAAVQVVLNAVMSANPIGLVVLAIAALVAGLVWAYNN VGWFKDFVDQAFAAIGAVVAAVAQWFQDAWNNAVTFVQAYIEAWSIIINAVFTGIQTA VGAVAQFFTDAWNNAVTFVQAYISAWGIIINAVFTGVQSAVGAVADFFRNAWAVAVAI VAGVIRSWQAGVNAVFNAVGSFISGVVNNVRNVFSSVFNVILGIVTGVIAGVRGAIDG VTSTVQSVASIINGALVAAFNFVASAGRNAFAGITGAIQGVIGWIQNALSWVRNLASG IGNAVGQMLGLGGATAATADAPGLSYFGGGDPGFEGGATSIFGGNTFFGAPAPKAAAP IIVNLTVNGAMDPTAVGKQIYDILLKYLRRNGDVVNGATPW" gene 9273..11090 /locus_tag="SEA_JESSICA_15" CDS 9273..11090 /locus_tag="SEA_JESSICA_15" /codon_start=1 /transl_table=11 /product="minor tail protein" /protein_id="ALF00770.1" /translation="MVTIQEAALTVNGGTYNPGKPAAFILPTAFSGLTVSYGGDSCAD HPGPGSISGRVFIPEQHSAFHPRIADPVHLRATINGDDMRMFYGTVDEIVIEDVDGEP LPAIIPNSRRMQSLDGWIVTTGATYEASLPTPATYLLDGARVSALGPTQGATANKLWF TTPAAPVSESRPYVVTAMAEAPSGLPALKAMWFNNAGGLIKIEDLYRWYTAGSFNGDF SPLRTQGTYPPVGAASVRIIVECELYANRESWQQACAVDGIVLHELPYGTVELPQLKA DKRPPGRWVTFKASDILATAARLIVGTTPWPSQTVEGRTAALNALVPAGAVTFNEGGT RDPFRLLGPRDIDKQNMLEIFQRVLASSGDLAVASSNFARYVVAASLPRYPQIIERIN GMATIVNDPLVPVLPAGSIVAGPMQTDITTMANQIRVEYRVVTDTMEQTGDDASAVYV NTESLAAYGAMGRSISTDLATVAGSRAEDKARRLAESQAQPFYRLADKVRLVSSQIPE APNVARLYSADIGFGQLVYVPDAPAVLGNYHRVRGATLTLGREPAVELDVEPPDYSAP EALTFGEARNTTPPFNILKLSEFKNITIGQLKYVSALEE" gene 11095..11610 /locus_tag="SEA_JESSICA_16" CDS 11095..11610 /locus_tag="SEA_JESSICA_16" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ALF00771.1" /translation="MDLSGAFPKLTDADSVYALKEYSERMFTELDKMPRGIVARSNLN GSTAGIGAAVMVDLVAVPLVAGRWYKVEYVFTSVAGGPNDAIAYDFKKSAVNDSTANG TSLNDGSQRFVYTGPAAGNSKTETVRTMWKATSNETQNIKAILARATGSVAFTANSRG LYVFDMGTTAP" gene 11607..12053 /locus_tag="SEA_JESSICA_17" CDS 11607..12053 /locus_tag="SEA_JESSICA_17" /codon_start=1 /transl_table=11 /product="lysin A peptidase domain" /protein_id="ALF00772.1" /translation="MTMTLAYPLAEGETIQEFGARRSFYRKLGQLGHNGIDLGCPVGT PVYAMAAGTVLHAGWSHDHPWLTHHAGIAVLTHHGEHISGLAHLSKVVVSPGERVEVG QLIGYSGDTGTAGVPHVHCELLAAQPDWSNGYAGRIRFEFTKGELS" gene 12050..12691 /locus_tag="SEA_JESSICA_18" CDS 12050..12691 /locus_tag="SEA_JESSICA_18" /codon_start=1 /transl_table=11 /product="lysin A amidase domain" /protein_id="ALF00773.1" /translation="MTYQYLTGQTSPFQSPRTQPIQSITIHWWDKPERNPSFQGTVDW LCRVGTTASIHYVAEAGRVACLVSPDNIAWHAGDGGNGPGNNTSIGIECNPRQSDGDY ATVAELVRDLRAVYGNLPIYPHRHWTSTECPGTYDLARINRLAATPAPSQEDQMNPEQ NRMLVAIYNALFNKKSMPTPDNQSIVGGEALDELINNNDVKILAKLEEINRKL" gene 12697..12906 /locus_tag="SEA_JESSICA_19" CDS 12697..12906 /locus_tag="SEA_JESSICA_19" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ALF00774.1" /translation="MTAKPTPKVAAVGVSGALTVLIVWVAGLCGIDMPAEVAAAISVV VTFGAGYIKSEVTERDGKRGEHVAR" gene 12896..13153 /locus_tag="SEA_JESSICA_20" CDS 12896..13153 /locus_tag="SEA_JESSICA_20" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ALF00775.1" /translation="MPDSGTWTQPEVVRSLQRIERKLDNAATSGYVEAIKADQLRKDT EQDKAIESVEQNYNKLLLMVVGTAIGSAASLLVTLASALPK" gene 13221..13475 /locus_tag="SEA_JESSICA_21" CDS 13221..13475 /locus_tag="SEA_JESSICA_21" /codon_start=1 /transl_table=11 /product="helix-turn-helix DNA binding protein" /protein_id="ALF00776.1" /translation="MASKAKCETTEYAGMLRRMIRAYGRRVGDADVEDLAVMLEVQRE LDAAIQSAVDSQRENHGRSWADIARATGTSRQAAQKKYGV" gene complement(13504..13776) /locus_tag="SEA_JESSICA_22" CDS complement(13504..13776) /locus_tag="SEA_JESSICA_22" /note="Helix-Turn-Helix DNA binding domain (XRE-like)" /codon_start=1 /transl_table=11 /product="helix-turn-helix DNA binding domain protein" /protein_id="ALF00777.1" /translation="MTTRVQARPASTDADIGKRIERRLSALGMTQWDLAARLGLTQAT VSRKLHGQRPWFASELVTVAGVLGCAVGELFGERCRPAVRPNVARI" gene 13913..14119 /locus_tag="SEA_JESSICA_23" CDS 13913..14119 /locus_tag="SEA_JESSICA_23" /note="HTH DNA binding protein, MerR-like" /codon_start=1 /transl_table=11 /product="MerR-like HTH DNA binding protein" /protein_id="ALF00778.1" /translation="MTEQSNETTELVEADRAAELLGVSKRTLDRYQAAGLLTPIRPIQ GKGAIRRFDAQDVQRLAVAQDVQP" gene 14116..14784 /locus_tag="SEA_JESSICA_24" CDS 14116..14784 /locus_tag="SEA_JESSICA_24" /note="Helix-Turn-Helix DNA binding domain" /codon_start=1 /transl_table=11 /product="helix-turn-helix DNA binding domain protein" /protein_id="ALF00779.1" /translation="MSIESMAVVLHHSQAGGTDKLVLLGIANHDGDGGSWPSVATLAR YANVEPRAVKACIKRLVDRGEVERERQAGGTRNMPDYTRPNLYHIKVVCPPECDRSAQ HRINRKDPVSSTTPGVGQIPPGGYVPDTPGGVRPTTPKPSLNHPSNTDKSPSSSTSPA VNGKLPCWNCGEHVIANTTKPKRYCQSCSSRGLDNPLIPCKKCGSVRKRSYPGEQEFD CGCV" gene 14771..14986 /locus_tag="SEA_JESSICA_25" CDS 14771..14986 /locus_tag="SEA_JESSICA_25" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ALF00780.1" /translation="MDAFEPYETYSMAVLWNMSAQTAHDAPVDGDALARSNWQTLSIQ RQWERLTPCQVQKIRDNHHEVDRDSRS" gene 14983..15276 /locus_tag="SEA_JESSICA_26" CDS 14983..15276 /locus_tag="SEA_JESSICA_26" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ALF00781.1" /translation="MTGLSQTTVDQAKQTAVNMEAIANSYQKTMEWNRQEYIKDATTD KWPQYIAALSEWQIHAQRATTARLMYEAIAHAYHLTEVWARCKALPTAADAAR" gene 15266..15529 /locus_tag="SEA_JESSICA_27" CDS 15266..15529 /locus_tag="SEA_JESSICA_27" /codon_start=1 /transl_table=11 /product="HNH endonuclease" /protein_id="ARE89882.1" /translation="MPGEWGGRAAQDLTKLCMDTYGWTCHLCKLPIRQGEQSADHLLP RKYGGSNDLSNLRPAHRKCNYARGAKLLSDPRARPTDNTAFFK" ORIGIN 1 ccgatctttt ttccccaact caaaacaaaa ggaggcccga aagtgaccga atacgccccg 61 atgctgcccg ggtttgaagc tccaaagacc ggcatgagca agctcgaaca gcgttgctcc 121 cagcaccttg tgatgctcca agagctgggt cttttgaaag accaggatca agtcatggcg 181 cagctcgtca tggatcttgc ccatgccgtc gcgctcagtg cagccgccgg caaggcagcc 241 ggcacagccc tagccgtcaa gccgctcatg gaagcacttg acaagctccc caaaccagta 301 accgaagatg cgtttgccgc aatgatgaaa gaggccggcc tagtatgagc accaaccccg 361 cagaactcac gttcgatatg aacttccacg ctttcgtctt ggctgtcccg ttgcggacca 421 cagaggccgg catgatgctt gggcagccgg tgatagcaat gaaccaaggc ggcgaggccc 481 agcttgtcat ggcgctacgt gcgatagccg atgacattga ggcccggggg actgacgtag 541 tggggaagtg gaccctttga gcgctatgga cgcggcaacc gtggccgtgc ttgcaagtta 601 tggctcactc gtgctggcaa cggctgccgt ggtcctgtcg gtgctccgtg gcaggtgact 661 ttagggacca ccgcgtggac ggttggccca tgcagatgat gagtatgccc gtaggttggt 721 acatcgacgt aaacgagtca gccggcatcg agtacccggt gaacggcgac gagacagcac 781 aacgtaagga tggtcaattg tggtagtagc gcttgctgac gagctgcccg agctgctggc 841 ggctttggag caatccacgg cacggtatgc caccaagccg acacccgggg caccgaacga 901 gcttgggcag atccttggca ccgccaagct gcttggccgg cagctcatgc cgtggcagat 961 cgaagtggca cgcgtggcat cagagaaacg cgttgatgat ccacggcgtt accgttaccc 1021 gattgtggtg ctcacggtgc cccggcaatc cggcaagacg acgctcatgc gtacagtact 1081 tgctcaacgt gctttgaagt gccgtaaccg taaggcgttc tacacggccc aaacgggcaa 1141 ggacgctacg gcgcggtggc tggatcttgt caaggacatt gaggacggcc ccttgtccca 1201 gttcgtcagc aagcgcattg ctgccggcag ccaagcgctg acgttcccga cgggcagcac 1261 cattagcccg ttcgccccga cagccaagag cttgcacggc tataccccgc atgacgtgat 1321 gttggacgag atatttgccc atgacgcagc cgccggcaac gacctcatgg gcgctatccg 1381 gccggcccag ctcacgctac ccgacaagca actatggctt gtcagcacgg ccggcacggc 1441 tgacagtggt tttctgaaat cgtgggttga ccaaggccgg ctagccgtca aagacagcgg 1501 tgccggcatt gcctactttg agtggagtct tgctgacggg ctggacccgt atgaccccca 1561 aaactggctg ttccacccgg cagttggtca cacaatcact ttggaggatc ttgccgacga 1621 cgcggacagc caaagccagg gggagtggtt gcgcgcatat atgaaccgtt ggacgagcac 1681 gagcgaggca gtcattgacg tggcgaaatg ggacacgttg gccggcgcgt tggtgcccgt 1741 tccatgggca caagtgacgg tggcctacga ggtggcgcac gacaggagtt gcgcggcgat 1801 ctatgcgtgc tggaaagatc ccgagacagg caagccggcg ctaaagctcg tgcagcaagg 1861 cagcggtgcc gagtggcttg ccccggccgt cgccaagatc tacgtggaga accggcccaa 1921 ggcgataggg gccgacgacg gcggaccaac caaggcagtg accgacaact tgcgccggct 1981 gcccaatgcc cgtagcgggg gcaacggcgt cgaggtgaca accctgacag cccgggactt 2041 tgctaccgct tgcgttgcct acatggggca cgttgacgac ggcacaatac tccacgacgg 2101 cgacccgggg caccgtgcag ccgttgaggc agcggcaacc cggcccatgg gtgacagcaa 2161 ggtttttagc cgccgtcaca gtagaggccc aattccagag ctggtggcgg caaccgtagc 2221 gctacggcta catgagcaag cgccggccac ggcaccgcaa ccggcaatct acatgggacg 2281 agggaactag cgtgatccgg cttgacaaga ctcaattcag cattgttgtg ctttgcacct 2341 tgtgccctac gtggcgagca ctccacaacg acaaggctct agcgctggca gccggccgac 2401 ggcacaacct gacggcgcac gagggcgagg acaacacctt gtcagcggcc cgccagcaag 2461 cctacagggc acgcaaagcg gctgccgggg cataatccca gcggtgtaat tttgaaaccg 2521 ttacgggggc atacaggatt gtctgtatgc ccctatggaa taacccacta gccaagccgg 2581 ccggcattct tgcccgtcag gctgccgcca gcgtagatgt tctagccggc aacgtcgtgt 2641 catgggaata tgccgaacct gacccggctc cacgcgatca cttccaaact ctcaccctgg 2701 ctcacttgct tggcgtcgag tatgtcaaca tcgaccgcac ggcagccatg ggcattggtg 2761 ccgtggcaaa gttgcggaaa acggcatgcg gctttatcgg ccgtatgccc ctcattgctt 2821 accgtggcca cgacgtgctt gcccgtcaac ccaagatcgt tttgcagccc gaggccggcc 2881 ggccccggtt tgtcacgatg gcttgggtga cagaagcgct catgttctac ggcaaggctt 2941 ggtttaccgt tgaagaacgc tatgccgagg acggccggcc ggcacgcttg cgttgggttc 3001 ccgagtggaa agccgagttc aacaccgccg gccagctcgt gaaagcctac ggcgtggaca 3061 tagaccccgc tgacgtgatc cgcgtggacg gcattgacga gggtctgttg aactacgcgc 3121 agcccgtcct acgtgaagcc cgggcaatcg acattgccgc cggccgtgca tcagataacc 3181 cggtcccgag cattgacttg caccagaccg gcggcgaccc gctgaccaac gagcagatag 3241 acgcgctcat agagcgttgg gcatcgaacc ggcgagccaa aaacggcggc gtgagcttca 3301 ccaaccaaag cgttgaggca aaaaccatgg ggcagcccgt ggagcaactg cttatcgacg 3361 gccgcaacgt ggcagccttg aacattgccc gggcagccgg ctttccggcg tgggcagttg 3421 atgccagcgt aaacggcagc tccattacct acagcaacag ccctagccgc acgcgtgagc 3481 tgattgacta cgcgctttca ccgtatctgg aagctattgc cgctcgtttc agcatggatg 3541 acattctgcc ggccggcaca tggtgcaggt tcgactattc cgagctgttg cgcggcgact 3601 tcgctgcccg tatggatgct tacaaagtcg ccaaagatgc agaaatttac tcaacagagg 3661 agctacgcgc catggagctt ggccggccat tggaggtctc ggaatgaagc acgcatattt 3721 gaatctgtcc gccgggttgc tgacggcaag cgcgtcaacg cgcaccatca gcggcgagat 3781 tgtcgaatac ggggtagtgg gacacaccag ccttggcccg acgatctttg cggccggcag 3841 catcacagct cccacgccgt tgtccaaggt caagatgctt gtgcagcatg acaccgagcg 3901 cagtgtgggc tttctggact cgttggagga caacggcacc aagccgtttg ccgcgttcaa 3961 ggtgcctgac ggtgccgagg gcgacgacgc actaaccaag gcagccaacg gcactcgtga 4021 ttctttcagc gttggcgtgc atgttcagga atacagcttt gacgacgagg gcaacttgct 4081 cgtgcatgcg tcaacgctga aagaagtttc cctagtcacc atcccggcat ttgaaaacgc 4141 gctcgtgcat gacgtagccg caaacaggaa agaggcagta atgaccgtcg aagaaatgcg 4201 tgcccaggct ctcgccaacg cacagacccc cggcaacccg gccgtagccc ttgctgccgc 4261 tgctgccgaa aatgccccgt cgccggctga ggttaccccc agcgcccagc cggcgacggc 4321 accaacccgg cacgccaccg ttgccgaggc tcaggctgcc cctattcagg tcggtggccg 4381 tcgcggcatg gatctttcag ccgctgccaa catcgtcatt gagcacttgc gcaacggctt 4441 gccggcaacg cagctcagtg ccgcactgtc cgacgtggtg ccggccgatg atgccggaga 4501 gggttttctc cggcctacgt tcatcggtga gctgtggcag gcgttcaacg atgaccggcc 4561 gcttatcgac gcgttcggca ccccgggcaa gctgaccggc accaaggtct atggctggaa 4621 gtgggatctt gccaaccgtc ccaaggtcgg ccgctacgcc ggcaacaaga ccgaacttcc 4681 atccaacccg ctcaagactg tcccggctga atccgatgct caggacttcg ctgccggctg 4741 ggacgtggca cgcaagtaca tcgaccttgg cgcgtcggac ttcattgagt ccgtgttccg 4801 tggtgccacc gcggattacc gtttgcagac tgaaatctgg ttcggtgagc agatccttgc 4861 ggaggcaacc gaagttgccg gcgtgaccac ggtgttgggt gcgctgtctc agttcaacgt 4921 cgaggctgcc cgtatcggtg cccgtctttc gacgatccag tttggcgtag acgcatggga 4981 ggaatttatt aacctcccag aagcgcaggt gccgtggtgg ctgaaagcac agggcagcgt 5041 cgagctggac ggcatgaaag gccgcgccgg cggtgtatct ttcagcgcga acctgggtct 5101 tggtgccggc cagatccttg gcgcggacaa gcgagccgca acctactacg aggccggcag 5161 cgtacctatc cgcgtaacgg cgcaggacat cccgcgtggc ggcgttgacc ttggcgtctt 5221 tggctacgcc ggcgctatcg tgcatgaccc ccgggcaatc tgggtatctg acgacggact 5281 cgtgtagccc atgatcgtaa cggtagaaca ggttaggacg tggctagggc tgccggcatc 5341 ggacccggcc ctagaagatg ccacggccgc aaccaacgct ttcgttgagc gccttgggtt 5401 gccgatgcag cccaagatcg ttgacggcat tgccgtgctg gacgacgacg gcgcacaaat 5461 gttcgagccg gccgctgaca cggtgcttgg tgccaagatg ctggcggcac ggctctaccg 5521 ccggcgcaac agcccgagcg gagtcgaagc gatcaccgac gccggcacgt cttttgttgc 5581 ccggtatgac agcgacataa gccgttactt gaagcttgac ggctttgcgg ccccgagaat 5641 cggctaagcg catgcggact atgggaaatt ccctaaaaga cattgccgat atggtcacgg 5701 cggccggcgt gccggctgcc gttgaccccc gggacttgaa cctacccggg gcatgggtga 5761 cccccgggct tgtcagcttt gacgtgctgg acgtggacac agcatttatg acgtttgaca 5821 tttacctagt tgcccctgac cacggtgccg tccattcgct caacagtctt ggggacatgt 5881 tggcgaagat ccggccggcc ctacaggttg gtgaagccat gcccgtcatg gtgaacctcc 5941 ctaaccacgg tgccgacgcg ttgccggcgc tacttatcag catcgacgca caactaacgg 6001 aggactaacc acaatggcta ctgacgtaat tacagtcggc cccggccgct tcaccatcgg 6061 ttcagataca gagctgaccg tcttttccgg tcaggtgacc agcttgcggc tcgtgccgag 6121 cgtggacgtt ggcgactcca tctacgtgtt ggacggcggc gaagtgtccg gcgaccgtac 6181 cgagtcgtgg accgtgtccg gcacgatgtt gcaggacttt ggcgcgacca ccagcaagac 6241 cgaatggctg ttcgagcacc ggggcgaaga tatgccgttt gcctacgccc cgaacagtgc 6301 caggggcaag gagattaccg gcgtgctgac cgttgaagcc atcgaaattg gcggcgacgt 6361 aaagaccaag ccaacatctg actttgagtt caagcttgtt ggacctcccg caattggcac 6421 agtttccgcc ggctaatggg cacgaaactc tacgccgtgg ttggcggcgc gaagctcagg 6481 agcacgctga gaaaagccgg cgcagatatg aaagaacttt ctgccgtcaa ccgcgacgta 6541 gccaacatcg ttttgcccgt cgccagagca acagcaccaa ccacaagcgg gaagctgggt 6601 agtacagtcc gtgccggcgc tacccagaag tcagcaatca tccgtgtggg cagcgcaaaa 6661 gcaccctacg gcccagtggt gcattactgg cacaagggca actacacgcc caatccttgg 6721 gtgtcacttg ccgcacaaaa gaccgaaccg acatggcttg cccggtatca cgccggcata 6781 gagcgaatca ttaaccaagt aacaggagcg taaaagaatc atggcgcagc tatcagcccc 6841 gaaagtcatt gtcatgctgg aatcatccgg caccgacgag ctgacggagt acaccgttca 6901 gaccgataac cgtgacgcga ttcagtggga cgtgacccgg ccccgtcgct cgtggccggc 6961 gttcaacgaa gcacccatgc tttacatgac attccttgca tggcacgcga tgcaccggac 7021 cggcgcaaca aagctctcgc ttgacgagtt catgaaagac gccgttgagg tcaaggtact 7081 gtcagcggcc ggcaaggcaa tcgacccgac cgaggccgtt gccgaggacg tccttgtgga 7141 ccctacccag ccggtagccg ctatcgcctg atagtcgagc tagcgcttgc aaccaacatt 7201 gcaccgagcg tatgggctgc cgagtccacc gaagtaatag caacagcact caccattttg 7261 gaagaacgaa agtaggccgc aatgtcccgt acagccgttt tggccgttcg tatcgtcacg 7321 gagacaaaag aggccaacaa gggcattgac gacactgtta gcaagctgga caagtttgag 7381 cgcggcctag acaaggcggc attgccggcg gctgccgccg gcactgccgt gcttgcgttc 7441 gccaagaaaa ccggcgacat ggcgagcatt gcccagcaaa acgccggcgc tgttgacagc 7501 gtgttcaagg gcaacgccaa gaccgtcaac gagttcgccg cgacggctgc cgacaagctg 7561 ggactgtccg gcagcgctta ccagcaaatg gcatcggtga tcggttcaca gctcaaaaac 7621 atgggcgtgc ccatggatca ggttgccggg tccaccaacg acctcattgc caagggtgcc 7681 gacttggctg ccatgttcgg cggcacgacc tccgatgccg tagacgcgct atccagcttg 7741 ctccgaggcg agcgtgaccc catcgagaag tacggcgtca gcatcaatga cgcggcaatc 7801 caagccaaaa aagcagagct tgggcttgcc gggttgtccg gtgaagccga caagaacgca 7861 acactgaccg cgaccatggc actgttgcag aagcaaacag ccgacgcgac ggggcagttt 7921 gcgcgggaag ctgacagcgc agccggtgcc caggaacgag ctaacgcgaa gatccaagac 7981 gccggcgcga agcttggcag cgtattcctg ccggccatgg cagccgcagc cacggcagcc 8041 ggcggcatgg ctacatgggc atcagaaaac agcacagtgc tgctggtgct tgccgggatc 8101 attggcggcg tggccggcgc gatcctgctt atcaacgggg cactcaaagc atggcgagcc 8161 gctacggctg ccgtcgctgc cgtgcaagtg gtactcaacg ccgtaatgtc agccaaccca 8221 attggccttg tggtgcttgc cattgccgca ctcgtggccg gccttgtgtg ggcttacaac 8281 aacgtgggtt ggttcaaaga ctttgttgac caagcttttg ccgctatcgg tgccgtggtg 8341 gctgccgtcg cgcaatggtt ccaagacgcg tggaacaacg ctgtcacgtt cgtacaggcg 8401 tacatcgaag cttggtccat catcatcaac gctgtcttta ccgggataca gacggccgtc 8461 ggtgccgtgg cccaattctt caccgacgca tggaacaacg ctgtcacgtt cgtacaggcg 8521 tacatatccg cgtggggcat tatcatcaat gccgttttca ccggggtaca gtccgccgtc 8581 ggtgccgtgg ctgacttttt ccgcaacgcg tgggcggttg ccgtggcaat agtcgccggc 8641 gtaatccgct cgtggcaagc cggcgtaaac gccgtgttca acgccgtggg ctcatttata 8701 tccggcgtcg tcaacaatgt ccgcaacgtg ttttcctcag tgttcaacgt gattctaggg 8761 atcgtaaccg gcgtcattgc cggcgtgcga ggcgcaatag acggggtaac cagcaccgtg 8821 cagagcgtcg ccagcatcat caacggggca ctcgtggccg cgttcaactt tgtcgccagt 8881 gccggccgta acgcttttgc cggcatcacc ggggcaatcc aaggcgtcat tggctggatc 8941 caaaacgcgt tgagctgggt tcggaatctt gcaagcggca tcggcaacgc tgtcggccag 9001 atgttgggcc ttggcggggc gacggccgcg acggcggacg cccctggact cagctacttt 9061 ggcggcggcg accccgggtt tgagggcggc gccacaagca tctttggcgg gaacactttc 9121 tttggtgccc cggcacccaa ggcagcggcc ccgatcatcg tcaacctgac cgtaaacggg 9181 gcaatggacc cgacggccgt aggcaagcag atctacgaca ttctgctgaa gtacttgcgc 9241 cgtaacggtg acgtggtgaa tggagctacc ccatggtgac aatccaagag gcagcactga 9301 ccgtcaacgg cggaacttac aacccgggca agccggccgc gttcatcctg ccgacagcct 9361 ttagcggctt gacggtgagc tacggcggcg actcgtgcgc agaccacccc ggcccgggca 9421 gcatcagcgg ccgcgtgttc atcccagaac agcattccgc gtttcacccg cgcatagctg 9481 acccggtgca tttgcgtgcc accatcaacg gcgacgatat gcggatgttt tacggcaccg 9541 tggacgagat tgttatcgag gacgtggacg gcgagccgct gccggctatc atccccaaca 9601 gccggcggat gcaaagccta gacggctgga tagtgacgac aggggcaacc tacgaggctt 9661 cactgccgac gccggcaact tatctgctgg acggcgcacg cgtgtcagcc ttgggaccaa 9721 cccaaggcgc gacagctaac aagctatggt ttactacgcc ggcagcgccg gtgagtgagt 9781 cccggcccta tgtcgtgacc gccatggcag aagctccgag cggcttgccg gcgctcaaag 9841 caatgtggtt caacaacgcc ggcggcctaa ttaagatcga agatctttat cgctggtaca 9901 ccgccggcag tttcaacggc gacttttcac cgctgaggac tcaggggact tatcccccgg 9961 ttggtgccgc gtcggtaagg atcatcgtgg agtgcgaact atacgccaac cgggagagct 10021 ggcaacaagc ttgtgccgta gacggcatcg tgctccatga gctgccctat ggaacggtgg 10081 agctgcccca gctcaaagcg gacaagcgtc cacccggccg ttgggtgacg ttcaaggcgt 10141 cggacattct ggcgaccgct gcccgtctta tcgtggggac tactccatgg ccgtcacaaa 10201 ccgtcgaggg caggacggcg gcactcaatg cgcttgtgcc ggccggtgct gtcacgttca 10261 acgagggcgg cacgcgtgac ccgttccggc tgctgggacc ccgggacatt gacaagcaga 10321 acatgcttga aatcttccaa cgcgtgcttg catcatcggg agacttggcc gtggcgtcgt 10381 ctaactttgc ccgttacgtg gtggccgctt cgctgccccg ttacccgcaa atcatcgagc 10441 gcattaacgg aatggcgacg attgtcaatg acccgttggt tccggtgctg ccggccggca 10501 gtatcgtggc cggccctatg cagaccgaca taacgaccat ggcaaatcag atccgcgtcg 10561 agtatcgggt ggtcactgac accatggagc aaaccggaga tgatgccagc gccgtctacg 10621 tcaacaccga gtcattggcg gcctacggtg ccatggggcg aagcatcagt acagaccttg 10681 ccaccgtggc cggcagccgc gccgaggaca aggcacgccg gcttgccgag agccaggcgc 10741 aaccgttcta ccggcttgcc gacaaggtgc ggcttgtgtc ctcacagatc ccggaagcgc 10801 cgaacgtggc acggctttac agcgccgaca ttgggttcgg gcagcttgtc tatgtgcccg 10861 acgcgccggc cgtgcttggc aactatcacc gagtcagggg agcaaccctg acgttggggc 10921 gagagccggc cgttgagctg gacgttgagc cgccggatta ttcggcaccc gaggcgctca 10981 cgtttggcga agcacgcaac accacacccc cgttcaacat cttgaagctg tccgagttca 11041 aaaacatcac catagggcag ctcaaatacg tcagcgcatt ggaggaataa gagcatggat 11101 ctatcagggg cattcccgaa actgaccgac gcggatagcg tctacgcgct caaggaatac 11161 agcgaacgca tgtttaccga gctggacaag atgccacgcg ggattgtggc acgttccaat 11221 ctcaatggct caacggccgg catcggtgcc gccgtcatgg tggatcttgt ggccgtgccg 11281 ctcgtggccg gccgctggta caaggttgag tatgtcttta ccagcgtggc cggcggtccc 11341 aatgacgcca ttgcctatga cttcaaaaag tcggcagtga acgactcaac agcgaacggc 11401 acgtcattga atgacggctc ccagcgcttt gtctacaccg gccccgctgc cggcaactca 11461 aagactgaga cagtccgcac tatgtggaaa gccaccagca acgaaacgca aaacatcaag 11521 gcaatccttg cccgggcaac aggcagcgtg gctttcaccg ccaacagtcg tggcctttac 11581 gtattcgata tggggactac agcaccatga caatgaccct ggcttatccc cttgctgagg 11641 gcgagacaat ccaagagttc ggtgcccgtc gatcgttcta ccggaagtta ggacagcttg 11701 ggcataacgg catcgacctt gggtgcccgg tcggcacccc cgtctatgcc atggcagccg 11761 gcacggtgct ccacgccggt tggtcccacg atcacccatg gctgacccat cacgccggca 11821 ttgctgtgct gacccatcac ggcgagcaca tcagcggcct agcgcaccta tccaaggtgg 11881 tggtgtcacc cggcgaacgt gtcgaggttg ggcagctcat tggctactca ggggacaccg 11941 ggaccgccgg cgttccgcat gtccattgcg agctgctggc agcacagccg gattggtcca 12001 acggctacgc cggccgtatc cgctttgagt tcactaaagg agagctatcg tgacatacca 12061 atatctgacc ggacagacaa gcccgttcca gtcgccccgg acgcagccca ttcagtcgat 12121 cactattcat tggtgggaca agcccgaacg taacccgtcg tttcagggca ccgtcgattg 12181 gctttgccgg gttggcacca cggccagcat tcattacgtg gccgaggccg gccgcgtggc 12241 gtgccttgtc tcacctgaca acattgcatg gcacgccggc gacggcggca acggcccggg 12301 caacaacacc agtattggca ttgagtgcaa cccgcgtcag tccgacggcg actacgccac 12361 ggtggccgag ttggtccgtg acctgcgagc cgtctacggc aatttgccga tctatcctca 12421 ccggcactgg acaagcacgg agtgccctgg aacctatgac cttgcccgaa tcaaccgact 12481 agccgcaacg ccggcaccat cacaggagga ccaaatgaac cccgaacaaa accgcatgct 12541 cgttgcgatc tacaacgcac tcttcaacaa gaagagcatg ccgacgccgg ataatcagtc 12601 catcgtcggc ggtgaagcac ttgacgagtt gataaacaac aatgacgtca agatccttgc 12661 caagctcgaa gagatcaacc gaaagctcta aggaccatga cagccaagcc aacccccaaa 12721 gtagcggccg tcggtgtcag cggcgctctg acagtcttga tcgtttgggt agcgggacta 12781 tgcggcattg atatgccggc agaagtcgcg gccgctatct cagtagttgt gacgttcggt 12841 gccggctaca tcaagagcga ggtgacagaa cgtgatggaa agcgaggtga gcacgttgcc 12901 cgatagcggc acatggacgc aacccgaggt tgtgcgctcg ttacaacgca ttgagcgcaa 12961 gctagacaac gctgccacaa gcggctacgt cgaggcgatc aaagcagacc agttgcgcaa 13021 ggataccgag caagacaagg caattgagtc ggtagaacag aactacaaca aactgttgct 13081 aatggtggtc ggcacggcca taggcagcgc ggcaagcctg cttgttacct tggcgtcagc 13141 cctacctaaa taggccagaa tgccccgtaa cacctaacaa gggttgcggg gcattttgct 13201 gtcaacgtag agttgacagc atggcaagca aagctaagtg tgaaacgacc gaatatgccg 13261 gcatgctacg gcgcatgatc cgcgcctatg gccggcgcgt cggtgatgct gacgtagagg 13321 acttggccgt gatgctggaa gtgcagcgag agcttgacgc ggctatccaa tcggccgtgg 13381 acagccagcg tgagaaccat ggcaggagtt gggcggacat tgcccgggcg accggcacca 13441 gccgccaagc tgcccagaag aaatacggcg tataacaaat tttggatatt gacttatagg 13501 ttcttatatc cgcgcgacgt tgggccggac tgccggccgg caccgttccc cgaatagctc 13561 accaacagcg cacccaagca cgccggcgac cgtcacaagc tccgaggcga accatgggcg 13621 ctgcccatgc agtttgcggc tgactgtggc ttgtgtcagc ccgagtcgcg cggcaagatc 13681 ccattgagtc atgcccagcg ccgatagccg gcgctctatg cgctttccta tgtcggcatc 13741 agtgcttgcc ggccgcgctt ggactcgtgt tgtcatggcg caaggttatg ccaagtaatg 13801 acattaatca ctacttgttt acgtcaatcg agtactcatt tatcggcccc cgctacttgg 13861 ttggcgtgtc gtaacttgtc gtttcatgcc agaggggtat agcgttcagg gcatgacaga 13921 gcaatcaaac gaaacgacag aacttgtcga agctgaccga gcggccgagc tactgggagt 13981 cagcaagcga acgttggaca ggtatcaagc cgccggccta ctgaccccaa tccggcccat 14041 tcagggaaag ggcgcaatac gccggtttga cgctcaggac gtgcagcggc ttgctgtagc 14101 tcaggacgtg cagccatgag cattgagtca atggccgtag tgcttcacca cagccaagcc 14161 ggcggcactg acaagctggt gctgctgggt atcgccaacc acgacggcga cggcggatct 14221 tggccgagcg tggcaaccct ggcacgctat gccaacgttg agccgcgtgc agtcaaggcg 14281 tgcatcaagc gtcttgttga taggggcgag gttgagcgag agcgacaagc cggcggcacc 14341 cgtaacatgc ccgactacac ccggccgaac ctttaccaca tcaaagtagt ctgtccgccg 14401 gaatgtgacc gtagcgcaca gcaccgaatc aacagaaaag acccggtgtc gtcaacgaca 14461 cccggtgtcg gccagatacc cccggggggg tacgttccag atacccccgg gggggtacgc 14521 ccaacgacac ccaaaccatc actaaaccat ccctctaaca ctgataaatc acctagctct 14581 agtacctcac cagcggtcaa cggcaaattg ccgtgctgga actgtgggga gcacgtcatt 14641 gcgaacacca ccaagccgaa acgctactgc cagtcatgca gctcacgcgg actggataac 14701 ccattgatcc catgcaagaa atgcggctca gtgcgaaagc gcagctaccc gggagaacag 14761 gagttcgact gtggatgcgt ttgagccata cgaaacctac tccatggccg tcctatggaa 14821 catgagcgca caaaccgccc atgatgcccc ggtagacggc gacgcgctgg caagaagcaa 14881 ttggcagact ctcagcattc aacggcagtg ggaacggctc acgccgtgcc aagttcagaa 14941 aatcagagac aaccatcacg aagtagacag ggatagcaga tcatgaccgg actcagccaa 15001 accaccgtgg accaagccaa gcagacggcc gtcaacatgg aagccattgc caactcatat 15061 cagaagacca tggagtggaa ccgtcaggag tacatcaaag acgcgaccac ggataagtgg 15121 cctcagtaca tagccgcact cagtgaatgg cagatacatg cccaacgcgc taccacggcg 15181 cggctcatgt atgaagccat tgcccacgcc taccacctga ccgaggtatg ggcacggtgc 15241 aaggcgctgc ccaccgctgc cgatgctgcc cggtgaatgg ggaggacgtg cggcccaaga 15301 cctcaccaag ctgtgcatgg atacctatgg gtggacgtgc cacctatgca agctgcccat 15361 caggcaaggt gagcagagcg cagaccacct attgccacgc aagtacggcg gaagcaatga 15421 cctatcgaac ctcagaccag ctcaccgcaa atgtaactat gcacgcggtg ccaagttgct 15481 gtcagatcca agagcaagac caacagacaa caccgcattt tttaagtagg ggcggctctg 15541 gacaccccgc gccacc //