LOCUS KU160658 15524 bp DNA linear PHG 19-JAN-2016 DEFINITION Arthrobacter phage Muttlie, complete genome. ACCESSION KU160658 VERSION KU160658.1 KEYWORDS . SOURCE Arthrobacter phage Muttlie ORGANISM Arthrobacter phage Muttlie Viruses; dsDNA viruses, no RNA stage; Caudovirales; Siphoviridae; Decurrovirus; unclassified Decurrovirus. REFERENCE 1 (bases 1 to 15524) AUTHORS Pizzorno,M.C., Stowe,E.L., Andolino,C.J., Benjamin,A.K., Blasi,C.J., Forster,G.L., Garrett,A.H., Le,A.T., Li,S., Lu,I., Luftman,N.D., McGinnis,J.H., Medrano,J.E., Meredith,S.M., Otero,K.L., Pinamont,W.J., Pirnie,R.T., Yankauskas,J.R., Bradley,K.W., Asai,D.J., Bowman,C.A., Russell,D.A., Pope,W.H., Jacobs-Sera,D., Hendrix,R.W. and Hatfull,G.F. TITLE Direct Submission JOURNAL Submitted (15-NOV-2015) Dept. of Biological Sciences and Pittsburgh Bacteriophage Institute, University of Pittsburgh, Crawford Hall, Pittsburgh, PA 15260, USA COMMENT Phage isolation, DNA preparation, and annotation were performed at Bucknell University. Sequencing by Illumina to approximately 9830x coverage was performed at the Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, PA. Arthrobacter phage Muttlie has defined ends with an 11 bp 3'overhang, CCCGCGCCACC. Assembly was performed by Newbler and Consed software as of Feb, 2015. Sequence and Annotation QC performed at the University of Pittsburgh. Supported by the Science Education Alliance, Howard Hughes Medical Institute, Chevy Chase, MD. ##Assembly-Data-START## Assembly Method :: Newbler and Consed v. Feb-2015 Coverage :: 9830x Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..15524 /organism="Arthrobacter phage Muttlie" /mol_type="genomic DNA" /isolation_source="soil" /db_xref="taxon:1772309" /lab_host="Arthrobacter sp. ATCC 21022" /country="USA" /lat_lon="39.893703 N 75.631470 W" /collection_date="01-Oct-2014" /collected_by="B. Pinamont" /identified_by="B. Pinamont" gene 43..348 /gene="1" /locus_tag="MUTTLIE_1" CDS 43..348 /gene="1" /locus_tag="MUTTLIE_1" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ALY09757.1" /translation="MTEYAPMLPGFEAPKTGMSKLEQRCSQHLVMLQELGLLKDQDQV MAQLVMDLAHAVALSAAAGKAAGTALAVKPLMEALDKLPKPVTEDAFAAMMKEAGLV" gene 345..560 /gene="2" /locus_tag="MUTTLIE_2" CDS 345..560 /gene="2" /locus_tag="MUTTLIE_2" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ALY09758.1" /translation="MSTNPAELTFDMNFHAFVLAVPLRTTEAGMMLGQPVIAMNQGGE AQLVMALRAIADDIEARGTDVVGKWTL" gene 591..806 /gene="3" /locus_tag="MUTTLIE_3" CDS 591..806 /gene="3" /locus_tag="MUTTLIE_3" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ALY09759.1" /translation="MQVMAHSCWQRLPWSCRCSVAGDFRDHRVDGWPMQMMSMPVGWY IDVNESAGIEYPVNGDETAQRKDGQLW" gene 800..2290 /gene="4" /locus_tag="MUTTLIE_4" CDS 800..2290 /gene="4" /locus_tag="MUTTLIE_4" /codon_start=1 /transl_table=11 /product="terminase large subunit" /protein_id="ALY09760.1" /translation="MVVALADELPELLAALEQSTARYATKPTPGAPNELGQILGTAKL LGRQLMPWQIEVARVASEKRVDDPRRYRYPIVVLTVPRQSGKTTLMRTVLAQRALKCR NRKAFYTAQTGKDATARWLDLVKDIEDGPLSQFVSKRIAAGSQALTFPTGSTISPFAP TAKSLHGYTPHDVMLDEIFAHDAAAGNDLMGAIRPAQLTLPDKQLWLVSTAGTADSGF LKSWVDQGRLAVKDSGAGIAYFEWSLADGLDPYDPQNWLFHPAVGHTITLEDLADDAD SQSQGEWLRAYMNRWTSTSEAVIDVAKWDTLAGALVPVPWAQVTVAYEVAHDRSCAAI YACWKDPETGKPALKLVQQGSGAEWLAPAVAKIYVEHRPKAIGADDGGPTKAVTDNLR RLPNARSGGNGVEVTTLTARDFATACVAYMGHIDDGTILHDGDPGHRAAVEAAATRPM GDSKVFSRRHSRGPIPELVAATVALRLHEQAPATAPQPAIYMGRGN" gene 2292..2495 /gene="5" /locus_tag="MUTTLIE_5" CDS 2292..2495 /gene="5" /locus_tag="MUTTLIE_5" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ALY09761.1" /translation="MIRLDKTQFSIVVLCTLCPTWRALHNDKALALAAGRRHNLTAHE GEDNTLSAARQQAYRARKAAAGA" gene 2547..3707 /gene="6" /locus_tag="MUTTLIE_6" CDS 2547..3707 /gene="6" /locus_tag="MUTTLIE_6" /codon_start=1 /transl_table=11 /product="portal protein" /protein_id="ALY09762.1" /translation="MPLWNNPLAKPAGILARQAAASVDVLAGNVVSWEYAEPDPAPRD HFQTLTLAHLLGVEYVNIDRTAAMGIGAVAKLRKTACGFIGRMPLIAYRGHDVLARQP KIVLQPEAGRPRFVTMAWVTEALMFYGKAWFTVEERYAEDGRPARLRWVPEWKAEFNT AGQLVKAYGVDIDPADVIRVDGIDEGLLNYAQPVLREARAIDIAAGRASDNPVPSIDL HQTGGDPLTNEQIDALIERWASNRRAKNGGVSFTNQSVEAKTMGQPVEQLLIDGRNVA ALNIARAAGFPAWAVDASVNGSSITYSNSPSRTRELIDYALSPYLEAIAARFSMDDIL PAGTWCRFDYSELLRGDFAARMDAYKVAKDAEIYSTEELRAMELGRPLEVSE" gene 3704..5287 /gene="7" /locus_tag="MUTTLIE_7" CDS 3704..5287 /gene="7" /locus_tag="MUTTLIE_7" /codon_start=1 /transl_table=11 /product="major capsid & capsid maturation protease" /protein_id="ALY09763.1" /translation="MKHAYLNLSAGLLTASASTRTISGEIVEYGVVGHTSLGPTIFAA GSITAPTPLSKVKMLVQHDTERSVGFLDSLEDNGTKPFAAFKVPDGAEGDDALTKAAN GTRDSFSVGVHVQEYSFDDEGNLLVHASTLKEVSLVTIPAFENALVHDVAANRKEAVM TVEEMRAQALANAQTPGNPAVALAAAAAENAPSPAEVTPSAQPATAPTRHATVAEAQA APIQVGGRRGMDLSAAANIVIEHLRNGLPATQLSAALSDVVPADDAGEGFLRPTFIGE LWQAFNDDRPLIDAFGTPGKLTGTKVYGWKWDLANRPKVGRYAGNKTELPSNPLKTVP AESDAQDFAAGWDVARKYIDLGASDFIESVFRGATADYRLQTEIWFGEQILAEATEVA GVTTVLGALSQFNVEAARIGARLSTIQFGVDAWEEFINLPEAQVPWWLKAQGSVELDG MKGRAGGVSFSANLGLGAGQILGADKRAATYYEAGSVPIRVTAQDIPRGGVDLGVFGY AGAIVHDPRAIWVSDDGLV" gene 5291..5647 /gene="8" /locus_tag="MUTTLIE_8" CDS 5291..5647 /gene="8" /locus_tag="MUTTLIE_8" /codon_start=1 /transl_table=11 /product="head-to-tail connector" /protein_id="ALY09764.1" /translation="MIVTVEQVRTWLGLPASDPALEDATDATNAFVERLGLPMQPKIV DGIAVLDDDGAQMFEPAADTVLGAKMLAARLYRRRNSPSGVEAITDAGTSFVARYDSD ISRYLKLDGFAAPRIG" gene 5652..6008 /gene="9" /locus_tag="MUTTLIE_9" CDS 5652..6008 /gene="9" /locus_tag="MUTTLIE_9" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ALY09765.1" /translation="MRTMGNSLKDIADMVTAAGVPAAVDPRDLNLPGAWVTPGLVSFD VLDVDTAFMTFDIYLVAPDHGAVHSLNSLGDMLAKIRPALQVGEAMPVMVNLPNHGAD ALPALLISIDAQLTED" gene 6015..6437 /gene="10" /locus_tag="MUTTLIE_10" CDS 6015..6437 /gene="10" /locus_tag="MUTTLIE_10" /codon_start=1 /transl_table=11 /product="major tail protein" /protein_id="ALY09766.1" /translation="MATDVITVGPGRFTIGSDTELTVFSGQVTSLRLVPSVDVGDSIY VLDGGEVSGDRTESWNVSGTMLQDFGATTSKTEWLFEHRGEDMPFAYAPNSARGKEIT GVLTVEAIEIGGDVKTKPTSDFEFKLVGPPAIGTVSAG" gene 6437..6814 /gene="11" /locus_tag="MUTTLIE_11" CDS 6437..6814 /gene="11" /locus_tag="MUTTLIE_11" /codon_start=1 /transl_table=11 /product="minor tail protein" /protein_id="ALY09767.1" /translation="MGTKLYAVVGGAKLRSTLRKAGADMKELSAVNRDVANIVLPVAR ATAPTTSGKLGSTVRAGATQKSAIIRVGSAKAPYGPVVHYWHKGNYTPNPWVSLAAQK TEPTWLARYHAGIERIINQVTGA" gene 6821..7171 /gene="12" /locus_tag="MUTTLIE_12" CDS 6821..7171 /gene="12" /locus_tag="MUTTLIE_12" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ALY09768.1" /translation="MAQLSAPKVVVMLESSGTDELTEYTVQTDNRDAIQWDVTRPRRS WPAFNEAPMLYMTFLAWHAMHRTGATKLSLDEFMKDAVEVKVLSAAGKAIDPTEAVAE DVLVDPTQPVAAIA" gene 7282..9279 /gene="13" /locus_tag="MUTTLIE_13" CDS 7282..9279 /gene="13" /locus_tag="MUTTLIE_13" /codon_start=1 /transl_table=11 /product="tapemeasure protein" /protein_id="ALY09769.1" /translation="MSRTAVLAVRIVTETKEANKGIDDTVSKLDKFERGLDKAALPAA AAGTAVLAFAKKTGDMASIAQQNAGAVDSVFKGNAKTVNEFAATAADKLGLSGSAYQQ MASVIGSQLKNMGVPMDQVAGSTNDLIAKGADLAAMFGGTTSDAVDALSSLLRGERDP IEKYGVSINDAAIQAKKAELGLAGLSGEADKNATLTATMALLQKQTADATGQFAREAD SAAGAQERANAKIQDAGAKLGSVFLPAMAAAATAAGGMATWASENSTVLLVLAGIIGG VAGAILLINGALKAWRAATAAVAAVQVVLNAVMSANPIGLVVLAIAALVAGLVWAYNN VGWFKDFVDQAFAAIGAVVAAVAQWFQDAWNNAVTFVQAYIEAWSIIINAVFTGVQSA VGAVAQFFTDAWNNAVTFVQAYIAAWGIIINAVFTGVQSAVGAVADFFRNAWAVAVAI VAGVIRSWQAGVNAVFNAVGSFISGVVNNVRNVFSSVFNVILGIVTGVIAGVRGAIDG VTSTVQSVASIINGALVAAFNFVASAGRNAFAGITGAIQGVIGWIQNALSWVRNLASG IGNAVGQMLGLGGATAATADAPGLSYFGGGDPGFEGGATSIFGGNTFFGAPAPKAAAP IIVNLTVNGAMDPTAVGKQIYDILLKYLRRNGDVVNGATPW" gene 9276..11090 /gene="14" /locus_tag="MUTTLIE_14" CDS 9276..11090 /gene="14" /locus_tag="MUTTLIE_14" /codon_start=1 /transl_table=11 /product="tail protein" /protein_id="ALY09770.1" /translation="MTIQEAALTVNGGTYNPGKPAAFILPTAFSGLTVSYGGDSCADH PGPGSISGRVFIPEQHSAFHPRIADPVHLRATINGDDMRMFYGTVDEIVIEDVDGEPL PAIIPNSRRMQSLDGWIVTTGATYEASLPTPATYLLDGARVSALGPTQGATANKLWFT TPAAPVSESRPYVVTAMAEAPSGLPALKAMWFNNAGGLIKIEDLYRWYTAGSFNGDFS PLRTQGTYPPVGAASVRIIVECELYANRESWQQACAVDGIVLHELPYGTVDLPQLKAD KRPPGRWVTFKASDILATAARLIVGTTPWPSQTVEGRTAALNALVPAGTVTFNEGGTR DPFRLLGPRDIDKQNMLEIFQRVLASSGDLAVASSNFARYVVAASLPRYPQIIERING MATIVNDPLVPVLPAGSIVAGPMQTDITTMANQIRVEYRVVTDTMEQTGDDASAVYVN TESLAAYGAMGRSISTDLATVAGSRAEDKARRLVQSQAQPFYRLADKVRLVSSQIPDA PNVARLYSADIGFGQLVYVPDAPAVLGNYHRVRGATLTLGREPAVELDVEPPDYSAPE ALTFGEARNTSPPFNILKLSEFKNITIGQLKYVSALEE" gene 11095..11610 /gene="15" /locus_tag="MUTTLIE_15" CDS 11095..11610 /gene="15" /locus_tag="MUTTLIE_15" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ALY09771.1" /translation="MDLSGAFPKLTDADSVYALKEYSERMFTELDKMPRGIVARSNLN GSTAGIGAAVMVDLVAVPLVAGRWYKVEHVFTSVAGGPNDAIAYDFKKSAVNDSTANG TSLNDGSQRFVYTGPAAGNSKTETVRTMFKATSNETQNIKAILARATGSVAFTANSRG LYVFDMGTTAP" gene 11607..12053 /gene="16" /locus_tag="MUTTLIE_16" CDS 11607..12053 /gene="16" /locus_tag="MUTTLIE_16" /codon_start=1 /transl_table=11 /product="endolysin" /protein_id="ALY09772.1" /translation="MTMTLAYPLAEGETIQEFGARRSFYRKLGQLGHNGIDLGCPVGT PVYAMAAGTVLHAGWSHDHPWLTHHAGIAVLTHHGEHISGLAHLSKVVVSPGERVEVG QLIGYSGDTGTAGVPHVHCELLAAQPDWSNGYAGRIRFEFTKGELS" gene 12050..12691 /gene="17" /locus_tag="MUTTLIE_17" CDS 12050..12691 /gene="17" /locus_tag="MUTTLIE_17" /codon_start=1 /transl_table=11 /product="endolysin" /protein_id="ALY09773.1" /translation="MTYQYLTGQTSPFQSPRTQPIQSITIHWWDKPERNPSFQGTVDW LCRVGTTASIHYVAEAGRVACLVSPDNIAWHAGDGGNGPGNNTSIGIECNPRQSDGDY ATVAELVRDLRAVYGNLPIYPHRHWTSTECPGTYDLARINRLAATPAPSQEDQMNPEQ NRMLVAIYNALFNKKSMPTPDNQSIVGGEALDELINNNDVKILAKLEEINRKL" gene 12697..12906 /gene="18" /locus_tag="MUTTLIE_18" CDS 12697..12906 /gene="18" /locus_tag="MUTTLIE_18" /codon_start=1 /transl_table=11 /product="holin" /protein_id="ALY09781.1" /translation="MTAKPTPKVAAVGVSGALTVLIVWVAGLCGIDMPAEVAAAISVV VTFGAGYIKSEVTERDGKRGEHVAR" gene 12887..13153 /gene="19" /locus_tag="MUTTLIE_19" CDS 12887..13153 /gene="19" /locus_tag="MUTTLIE_19" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ALY09774.1" /translation="MSTLPDSGTWTQPEVVRSLQRIERKLDNAATSGYVEAIKADQLR KDTEQDKAIESVEQNYNKLLLMVVGTAIGSAASLLVALASALPK" gene 13221..13475 /gene="20" /locus_tag="MUTTLIE_20" CDS 13221..13475 /gene="20" /locus_tag="MUTTLIE_20" /codon_start=1 /transl_table=11 /product="HTH DNA binding domain protein" /protein_id="ALY09775.1" /translation="MASKAKCETTEYAGMLRRMIRAYGRRVGDADVEDLAVMLEVQRE LDAAIQSAVDSQRENHGRSWADIARATGTSRQAAQKKYGA" gene complement(13472..13744) /gene="21" /locus_tag="MUTTLIE_21" CDS complement(13472..13744) /gene="21" /locus_tag="MUTTLIE_21" /codon_start=1 /transl_table=11 /product="HTH DNA binding domain protein" /protein_id="ALY09776.1" /translation="MTTRVQARPASTDADIGKRIERRLSALGMTQWDLAARLGLTQAT VSRKLHGQRPWFASELVTVAGVLGCAVGELFGERCRPAVRPNVARV" gene 13854..14087 /gene="22" /locus_tag="MUTTLIE_22" CDS 13854..14087 /gene="22" /locus_tag="MUTTLIE_22" /codon_start=1 /transl_table=11 /product="HTH DNA binding domain protein" /protein_id="ALY09777.1" /translation="MPEGYSVQGMTEQSNETTELVEADRAAELLGVSKRTLDRYQAAG LLTPIRPIQGKGAIRRFDAQDVQRLAVAQDVQP" gene 14084..14752 /gene="23" /locus_tag="MUTTLIE_23" CDS 14084..14752 /gene="23" /locus_tag="MUTTLIE_23" /codon_start=1 /transl_table=11 /product="HTH DNA binding domain protein" /protein_id="ALY09778.1" /translation="MSIESMAVVLHHSQAGGTDKLVLLGIANHDGDGGSWPSVATLAR YANVEPRAVKACIKRLVDRGEVERERQAGGTRNMPDYTRPNLYHIKVVCPPECDRSAQ HRINRKDPVSSTTPGVGQIPPGGYVPDTPGGVRSTTPKPSLNHPSNTDKSPSSSTSPA VNGKLPCWNCGEHVIANTTKPKRYCQSCSSRGLDNPLIPCKKCGSVRKRSYPGEQEFD CGCV" gene 14739..14954 /gene="24" /locus_tag="MUTTLIE_24" CDS 14739..14954 /gene="24" /locus_tag="MUTTLIE_24" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ALY09779.1" /translation="MDAFEPYETYSMAVLWNMSAQAAHDAPVDGDALARSNWQTLSIQ RQWERLTPCQVQKIRDNHHEVDRDSRS" gene 14951..15244 /gene="25" /locus_tag="MUTTLIE_25" CDS 14951..15244 /gene="25" /locus_tag="MUTTLIE_25" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ALY09780.1" /translation="MTGLSQATVDQAKQTAVNMEAIANSYQKTMEWNRQEYIKDATTD KWPQYIAALSEWQIHAQRATTARLMYEAIAHAYHLTEVWARCKALPTAADAAR" gene 15231..15497 /gene="26" /locus_tag="MUTTLIE_26" CDS 15231..15497 /gene="26" /locus_tag="MUTTLIE_26" /codon_start=1 /transl_table=11 /product="HNH endonuclease" /protein_id="ALY09782.1" /translation="MLPGEWGGREAQDLTKLCMATYGWTCHLCKLPIRQGEQSADHLL PRKYGGSNDLSNLRPAHRKCNYARGAKLLSDPRARPTDNTAFFK" ORIGIN 1 ccgatctttt ttccccaact caaaacaaaa ggaggcccga aagtgaccga atacgccccg 61 atgctgcccg ggtttgaagc tccaaagacc ggcatgagca agctcgaaca gcgttgctct 121 cagcaccttg tgatgctcca agagctgggt cttttgaaag accaggatca agtcatggcg 181 cagctcgtca tggatcttgc ccatgccgtc gcgctcagtg cagccgccgg caaggcagcc 241 ggcacagccc tagccgtcaa gccgctcatg gaagcacttg acaagctccc caaaccagta 301 accgaagatg cgtttgccgc aatgatgaaa gaggccggcc tagtatgagc accaaccccg 361 cagaactcac gttcgatatg aacttccacg ctttcgtctt ggctgtcccg ttgcggacca 421 cagaggccgg catgatgctt gggcagccgg tgatagcaat gaaccaaggc ggcgaggccc 481 agcttgtcat ggcgctacgt gcgatagccg atgacattga ggcccggggg actgacgtag 541 tggggaagtg gaccctttga gcgctatgga cgcggcaacc gtggccgtgc ttgcaagtta 601 tggctcactc gtgctggcaa cggctgccgt ggtcctgtcg gtgctccgtg gccggtgact 661 ttagggacca ccgcgtggac ggttggccca tgcagatgat gagtatgccc gtaggttggt 721 acatcgacgt aaacgagtca gccggcatcg agtacccggt gaacggcgac gagacagcac 781 aacgtaagga tggtcaattg tggtagtagc gcttgctgac gagctgcccg agctgctggc 841 ggctttggag caatccacgg cacggtatgc caccaagccg acacccgggg caccgaacga 901 gcttgggcag atccttggca ccgccaagct gcttggccgg cagctcatgc cgtggcagat 961 cgaagtggca cgcgtggcat cagagaaacg cgtggatgat ccacgccgtt accgttaccc 1021 gattgtggtg ctcacggtgc cccggcaatc cggcaagacg acgctcatgc ggacagtcct 1081 tgcccaacgt gctttgaagt gccgtaaccg taaggcgttc tacacggccc agacgggcaa 1141 agacgctacg gcgcggtggc tggatcttgt caaggacatt gaggacggcc cattgtccca 1201 gttcgtcagc aagcgcattg ctgccggtag ccaagcgctg acgttcccga cgggcagcac 1261 catcagcccg tttgccccga cagccaagag cttgcacggc tataccccgc atgacgtgat 1321 gttggacgag atatttgccc atgacgcagc cgccggcaac gacctcatgg gcgctatccg 1381 gccggcgcag ctcacactgc ccgacaagca actatggctt gtcagcacgg ccggcacggc 1441 tgacagtggc tttctgaaat cgtgggttga ccaaggccgg ctagccgtca aagacagcgg 1501 tgccggcatt gcatactttg agtggagtct tgctgacggt ctggacccgt atgaccccca 1561 aaactggctg ttccacccgg ccgttggtca cacaatcact ttggaggatc ttgccgacga 1621 tgccgacagc caaagccagg gggagtggtt gcgcgcatat atgaaccgtt ggacgagcac 1681 gagcgaggca gtcattgacg tggcgaagtg ggacacgttg gccggcgcgt tggtgcccgt 1741 tccatgggca caagtgacgg tggcctacga ggtggcgcac gacaggagtt gcgcggcgat 1801 ctatgcgtgc tggaaagatc ccgagacagg caagccggcg ctaaagctcg tgcagcaagg 1861 cagcggtgcc gagtggcttg ccccggccgt cgccaagatc tacgtggagc accggcccaa 1921 ggcgataggg gccgacgacg gcggaccaac caaggcagtg accgacaact tgcgccggct 1981 gcccaatgcc cgtagcgggg gcaacggcgt cgaggtaaca accctgacag cccgggactt 2041 tgctaccgct tgcgttgcct acatggggca cattgacgac ggcacaatac tccacgacgg 2101 cgacccgggg caccgtgcag ccgttgaggc agcggcaacc cggcccatgg gagacagcaa 2161 ggtctttagc cggcgtcaca gtagaggccc aattccagag ctggtggcgg caacagtagc 2221 gctacggcta catgagcaag cgccggccac ggcaccgcaa ccggcaatct acatgggacg 2281 agggaactaa cgtgatccgg cttgacaaga ctcaattcag cattgttgtg ctttgcacct 2341 tgtgcccgac gtggcgagca ctccacaacg acaaggctct agcgctggca gccggccgac 2401 ggcacaacct gacggcgcac gagggtgagg acaacacctt gtcagcggcc cgccagcaag 2461 cctacagggc acgcaaagcg gctgccgggg cataatccca gcggtgtaat tttgaaaccg 2521 ttacgggggc atacaggatt gtctgtatgc ccctatggaa taacccacta gccaagccgg 2581 ccggcattct tgcccgtcag gctgccgcca gcgtagatgt tctagccggc aacgtcgtgt 2641 catgggaata tgccgaacct gacccggccc cccgcgatca cttccaaact ctcaccttgg 2701 ctcacttgct tggcgtcgag tatgtcaaca tcgaccgcac ggcagccatg ggcattggtg 2761 ccgtggcgaa gttgcggaaa acggcatgcg gctttatcgg ccgtatgccc ctcattgctt 2821 accgtggcca cgacgtgctt gcccgtcaac ccaagatcgt tttgcagccc gaggccggcc 2881 ggccccggtt tgtcacgatg gcttgggtga cagaagcgct catgttctac ggcaaggcgt 2941 ggttcaccgt tgaagaacgc tatgccgagg acggccggcc ggcacgcttg cgttgggttc 3001 ccgagtggaa agccgagttc aacaccgccg gccagctcgt gaaagcctac ggcgtggaca 3061 tagaccccgc tgacgtgatc cgcgtggacg gcattgacga gggtctgttg aactacgcgc 3121 agcccgtcct acgtgaagcc cgcgcaatcg acattgccgc cggccgtgca tcagataacc 3181 cggtcccgag cattgacttg caccagaccg gcggcgaccc gctgaccaac gagcagatag 3241 acgcgctcat agagcgttgg gcatcgaacc ggcgagccaa aaacggcggt gtgagcttca 3301 ccaaccaaag cgttgaggcc aaaaccatgg ggcagcccgt cgagcaactg cttatcgacg 3361 gccgcaacgt ggcagccttg aacattgccc gggcagccgg ctttccggcg tgggcagttg 3421 atgccagcgt aaacggcagc tccattacct acagcaacag cccaagccgc acgcgtgagc 3481 tgattgacta cgcgctttca ccgtatctgg aagctattgc cgctcgtttc agcatggatg 3541 acattctgcc ggccggcact tggtgcaggt tcgactattc cgagctgttg cgcggcgact 3601 tcgctgcccg tatggatgct tacaaagtcg ccaaagatgc agaaatttac tcaacagagg 3661 agctacgcgc catggagctt ggccggccat tggaggtctc agaatgaagc acgcatattt 3721 gaatctgtcc gccgggttgc tgacggcaag cgcgtcaacg cgcaccatca gcggcgagat 3781 tgtcgaatac ggggtagtgg gacacaccag ccttggcccg acgatctttg cggccggcag 3841 catcacagcc cccacgccgt tgtccaaggt caagatgctt gtgcagcatg acaccgagcg 3901 cagtgtgggc tttctggact cgttggagga caacggcacc aagccgtttg ccgcgttcaa 3961 ggtgcctgac ggtgccgagg gcgacgacgc actaaccaag gcagccaacg gcactcgtga 4021 ttctttcagc gttggcgtgc atgttcagga atacagcttt gacgacgagg gcaacttgct 4081 cgtgcatgcg tcaacgctga aagaagtttc cctagtcacc atcccggcat ttgaaaacgc 4141 gctcgtgcat gacgtagccg caaacaggaa agaggcagta atgaccgtcg aagaaatgcg 4201 tgcccaggct ctcgccaacg cacagacccc cggcaacccg gccgtagccc ttgctgccgc 4261 tgctgccgaa aatgccccgt cgccggctga ggttaccccc agcgcccagc cggcgacggc 4321 accaacccgg cacgccaccg ttgccgaggc tcaggctgcc cctattcagg tcggtggccg 4381 gcgcggcatg gatctttcag ccgctgccaa catcgtcatt gagcacttgc gcaacggctt 4441 gccggccacg cagctcagtg ccgcactgtc cgacgtggtg ccggccgatg atgccggaga 4501 gggtttcctc cggcccacgt tcatcggtga gctgtggcag gcgttcaacg atgaccggcc 4561 gctcatcgac gctttcggca ccccgggcaa gctgaccggc accaaggtct atggctggaa 4621 gtgggatctt gccaaccgtc ccaaggtcgg ccgctacgcc ggcaacaaga ccgaacttcc 4681 atccaacccg ctcaagactg tcccggctga atccgatgct caggacttcg ctgccggctg 4741 ggacgtggca cgcaagtaca tcgaccttgg cgcgtcggac ttcattgagt ccgtgttccg 4801 tggtgccacc gcggattacc gtttgcagac tgaaatctgg ttcggtgagc agatccttgc 4861 cgaggcaacc gaagttgccg gcgtgactac ggtgttgggt gcgctgtctc agttcaacgt 4921 cgaggctgcc cgtatcggtg cccgtctttc gacgattcag tttggcgtag acgcgtggga 4981 ggaatttatc aacctcccag aagctcaggt gccgtggtgg ctgaaagcac agggcagcgt 5041 cgagctggac ggcatgaaag gccgcgccgg cggtgtatcg ttcagcgcga acctgggtct 5101 cggtgccggc cagatccttg gcgcggacaa gcgagccgca acctactacg aggccggcag 5161 cgtgcctatc cgcgtaacag cgcaggacat cccgcgtggc ggcgttgacc ttggcgtctt 5221 tggctacgcc ggcgctatcg tgcatgaccc ccgggcaatc tgggtatctg acgacggact 5281 cgtgtaaccc atgatcgtaa cggtagaaca ggttaggacg tggctagggc tgccggcatc 5341 ggacccggcc ctagaagatg ccacggacgc aaccaacgct ttcgttgagc gccttgggtt 5401 gccgatgcag cccaagatcg ttgacggcat tgccgtgctg gacgacgacg gcgcacaaat 5461 gttcgagccg gccgctgaca cggtgcttgg tgccaagatg ctggcggcac ggctctaccg 5521 ccggcgcaac agcccaagcg gagtagaagc gatcaccgac gccggcacgt cttttgttgc 5581 ccggtatgac agcgacataa gccgttactt gaagcttgac ggctttgcgg ccccgagaat 5641 cggctaagcg catgcggact atgggaaatt ccctaaaaga cattgccgat atggtcacgg 5701 cggccggcgt gccggctgcc gttgaccccc gggacttgaa cctacccggg gcatgggtga 5761 cacccgggct tgtcagcttt gacgtgctgg acgtggacac agcatttatg acgtttgaca 5821 tttacctagt tgcccctgac cacggtgccg tccattcgct caacagtctt ggggacatgt 5881 tggcgaagat ccggccggcc ctacaggttg gtgaagccat gcccgtcatg gtgaacctcc 5941 ctaaccacgg tgccgacgcg ttgccggcgc tacttatcag catcgacgca caactaacgg 6001 aggactaaac cacaatggct actgacgtaa ttacagtcgg ccccggccgc ttcaccatcg 6061 gttcagatac agagctgacc gtcttttccg gtcaggtcac cagcttgcgg ctcgtgccga 6121 gcgtggacgt tggcgactcc atctacgtgt tggacggcgg cgaagtgtcc ggcgaccgta 6181 ccgagtcgtg gaacgtgtcc ggcacgatgt tgcaggactt tggcgcgacc accagcaaga 6241 ccgaatggct gttcgagcac cggggcgaag atatgccgtt tgcctacgcc ccgaacagtg 6301 ccaggggcaa ggagattacc ggcgtgctga ccgttgaagc catcgaaatt ggcggcgacg 6361 taaagaccaa gcccacatct gactttgagt tcaagcttgt tggacctccc gcaattggca 6421 cagtttccgc cggctaatgg gcacgaaact ctacgccgtg gttggcggcg cgaagctcag 6481 gagcacgctg agaaaagccg gcgcagatat gaaagaactt tctgccgtca accgcgacgt 6541 agccaacatc gttttgcccg tcgccagagc aacagcacca accacaagcg ggaagctggg 6601 tagcacagtc cgagccggcg ctacccagaa gtcagcaatc atccgcgtgg gcagcgcaaa 6661 agcaccctac ggcccagtgg tgcactactg gcacaagggc aactacacac ccaatccttg 6721 ggtgtcactt gccgcacaaa agaccgagcc aacatggctt gcccggtatc acgccggcat 6781 agagcgaatc atcaaccaag taacaggagc ttaaagaatc atggcgcagc tatcagcccc 6841 gaaagtcgtt gtcatgctgg aatcatccgg caccgacgag ctgacggagt acaccgtcca 6901 gaccgataac cgtgacgcga ttcagtggga cgtgacccgg ccccgtcgct cgtggccggc 6961 gttcaacgaa gcacccatgc tttacatgac attccttgca tggcacgcga tgcaccggac 7021 cggcgcaaca aagctctccc ttgacgagtt catgaaagac gccgttgagg tcaaggtact 7081 gtcagcggcc ggcaaggcaa tcgacccgac cgaagccgtt gccgaggacg tccttgtgga 7141 ccctacccag ccggtagccg ctatcgcctg atagtcgagc tagcgctggc aaccaacatt 7201 gcaccaagcg tatgggcagc cgagtccacc gaagtaatag caacagcact caccattttg 7261 gaagaacgaa agtaggccgc aatgtcccgt acagccgtct tggccgttcg tatcgtcacg 7321 gagacaaaag aggccaacaa gggcattgac gacactgtta gcaagctgga caagtttgag 7381 cgcggcctag acaaggcggc attgccggcg gctgccgccg gcactgccgt gcttgcgttc 7441 gccaagaaaa ccggcgacat ggcgagcatt gcccagcaaa acgccggcgc tgttgacagt 7501 gtgttcaagg gcaacgccaa gaccgtcaac gagttcgccg cgacggctgc cgacaagctg 7561 ggactgtccg gcagcgctta ccagcaaatg gcatcggtca tcggttcaca gctcaaaaac 7621 atgggtgtgc ccatggatca ggttgccggg tccaccaacg acctcattgc caagggtgcc 7681 gacttagctg ccatgttcgg cggcacgacc tccgatgccg tggacgcgct atccagcttg 7741 ctccgaggcg agcgtgaccc catcgagaag tacggcgtca gcatcaacga cgcggcaatc 7801 caagccaaaa aagcagagct tgggcttgcc gggttgtccg gtgaagccga caagaacgca 7861 acactgaccg caaccatggc actgttgcag aagcagacag ccgacgcgac ggggcagttt 7921 gcgcgtgaag ctgacagcgc agccggtgcc caggaacgag ctaacgcgaa gatccaagac 7981 gccggcgcga agcttggcag cgtattcctg ccggccatgg cagccgcagc cacggcagcc 8041 ggcggcatgg ctacatgggc atcagaaaac agcacagtgc tgctggtgct tgccgggatc 8101 attggcggcg tggccggcgc gatcctgctt atcaacgggg cactcaaagc atggcgagcc 8161 gctacggctg ccgtcgctgc cgtgcaagtg gtactcaacg ccgtaatgtc agccaaccca 8221 attggccttg tggtgcttgc cattgccgca ctcgtggccg gccttgtgtg ggcttacaac 8281 aacgtgggtt ggttcaaaga ctttgttgac caagcttttg ccgctatcgg tgccgtggtg 8341 gctgccgtgg cgcaatggtt ccaagacgcg tggaacaacg ctgtcacgtt cgtacaggcg 8401 tacatcgaag cgtggtccat catcatcaac gccgtgttca ccggcgtaca gtccgccgtc 8461 ggtgccgtgg cccaattctt caccgacgcg tggaacaacg ctgtcacgtt cgtacaggcg 8521 tacatagccg cgtggggcat tatcatcaac gccgtgttca ccggcgtaca gtccgccgtc 8581 ggtgccgtgg ctgacttttt ccgcaacgcg tgggcggttg ccgtggcaat agtcgccggc 8641 gtaatccgct cgtggcaagc cggcgtcaac gccgtgttca acgccgtggg ctcatttata 8701 tccggcgtcg tcaacaatgt ccgcaacgtg ttttcctcag tgttcaacgt gattctaggg 8761 atcgtaaccg gcgtcattgc cggcgtgcga ggcgcaatag acggggtaac cagcaccgtg 8821 cagagcgtcg ccagcatcat caacggggca ctcgtggccg cgttcaactt tgtcgccagc 8881 gccggccgta acgcttttgc cggcatcacc ggggcaatcc aaggcgtcat tggctggatc 8941 caaaacgcgt tgagctgggt tcggaatctt gcaagcggca tcggcaacgc tgtcggccag 9001 atgttgggcc ttggcggggc gacggccgcg acagcggacg cccctggact cagctacttt 9061 ggcggcggtg accccgggtt tgagggcggc gcaacaagca tctttggcgg caacactttc 9121 tttggtgccc cggcacccaa ggcagcggca ccgatcatcg taaacctgac cgtaaacggg 9181 gcaatggacc cgacagccgt aggcaagcag atctatgaca ttctgctgaa gtacttgcgc 9241 cgtaacggtg acgtggtgaa tggagctacc ccatggtgac aatccaagag gcagcactga 9301 ccgtcaacgg cggaacgtac aacccgggca agccggccgc gttcatcctg ccgacagcct 9361 ttagcggctt gacggtgagc tacggcggcg actcgtgcgc agaccatccc ggcccgggca 9421 gcatcagcgg ccgcgtgttc atcccagaac agcattccgc gtttcacccg cgcatagctg 9481 acccggtgca tttgcgtgcc accatcaacg gcgacgatat gcggatgttt tacggcaccg 9541 tggacgagat tgttatcgag gacgtggacg gcgagccgct gccggctatc atccccaaca 9601 gccggcggat gcaaagccta gacggctgga tagtgacgac aggggcaacc tacgaggctt 9661 cactgccgac gccagcaact tatctgctgg acggcgcacg cgtgtcagcc ttgggaccaa 9721 cccaaggcgc gacagctaac aagctatggt tcacaacgcc ggccgcgccg gtcagtgagt 9781 cccggcccta tgtcgtgaca gccatggccg aagctccgag cggtttgccg gcgctaaaag 9841 caatgtggtt caacaacgcc ggcggcctaa ttaagatcga agatctttat cgctggtaca 9901 ccgccggcag tttcaacggc gacttttcac cgctgaggac tcaggggact tatcccccgg 9961 ttggtgccgc gtcggtaagg atcatcgtgg agtgcgaact atacgccaac cgggagagct 10021 ggcaacaagc ttgtgccgta gacggcatcg tgctccatga gctgccctat ggaacggtgg 10081 atctgcccca actcaaagcg gacaagcgtc cacccggccg ttgggtgacg ttcaaggcgt 10141 cggacattct ggcgaccgct gcccgtctta tcgtggggac tactccatgg ccgtcacaaa 10201 ccgtcgaggg caggacggcg gcactcaatg cgcttgtgcc ggccggcact gtcacgttca 10261 acgagggcgg cacgcgtgac ccgttccggc tgctgggacc ccgggacatt gacaagcaga 10321 acatgcttga aatcttccaa cgcgtgcttg catcatcggg agacttggcc gtggcgtcgt 10381 ctaactttgc ccgttacgtg gtggcggctt cgctgccccg ttacccgcaa atcatcgagc 10441 gcatcaacgg catggcaacg attgtcaatg acccgttggt gccggtgctg ccggccggca 10501 gtatcgtggc cggccctatg cagaccgaca taacgaccat ggcaaatcag atccgcgtcg 10561 aatatcgagt tgtcactgac accatggagc aaaccggaga tgatgccagc gccgtctacg 10621 tcaacaccga gtcattggcg gcctacggtg ccatggggcg aagcatcagc acagacctcg 10681 ccaccgtggc cggcagccgt gccgaggaca aggcacgccg gcttgtccag agccaggctc 10741 aaccgttcta ccgtctggcg gacaaggtcc ggcttgtgtc ctcacagatc ccggacgcgc 10801 cgaacgtggc acggctttac agcgccgata ttgggttcgg gcagcttgtc tatgttcccg 10861 acgcgccggc cgtgcttggc aactatcacc gagtcagggg agcaaccctg acgttggggc 10921 gagaaccggc cgttgagctg gacgttgagc cgccggatta ttcggcaccc gaggcgctca 10981 cgtttggcga agcacgcaac acctcacccc cgttcaacat cttgaagctg tccgagttca 11041 aaaacatcac catagggcag ctcaaatacg tcagcgcatt ggaggaataa gagcatggat 11101 ctatcagggg cattcccgaa actgaccgac gcggatagcg tctacgcact caaggaatac 11161 agcgaacgca tgtttaccga gctggacaag atgccacgcg ggattgtggc acgttccaat 11221 ctcaatggct caacggccgg catcggtgcc gccgtcatgg tggatcttgt ggccgtgccg 11281 ctcgtggccg gccgctggta caaggttgag catgtcttta ccagcgtggc cggcggtccc 11341 aatgacgcca ttgcctatga cttcaaaaag tcggcagtga acgactcaac agccaacggc 11401 acgtcattga atgacggctc acagcgcttt gtctacaccg gccccgctgc cggcaactca 11461 aagactgaga cagtccgcac catgttcaaa gccaccagca acgaaacgca aaacatcaag 11521 gcaatccttg cccgggcaac aggcagcgtg gctttcaccg ccaacagccg tggcctttac 11581 gtattcgata tggggactac agcaccatga caatgaccct ggcttatccc cttgctgagg 11641 gcgagacaat ccaagagttc ggtgcccgtc gctcgttcta ccggaagtta ggacagcttg 11701 ggcataacgg catcgacctt gggtgcccgg tcggcacccc cgtctatgcc atggcagccg 11761 gcacggtgct ccacgccggc tggtcccacg atcacccatg gctgacccat cacgccggca 11821 ttgctgtgct gacccatcac ggcgagcaca tcagcggcct agcgcaccta tccaaggtgg 11881 tggtgtcacc cggcgaacgt gtcgaagttg ggcagctcat tggctactca ggggacaccg 11941 ggaccgccgg cgttccgcat gtccattgcg agctgctggc agcacagccg gattggtcca 12001 acggctacgc cggccgtatc cgctttgagt tcactaaagg agagctatcg tgacatacca 12061 atatctgacc ggacagacaa gcccgttcca gtcgccccgg acacagccca ttcagtcgat 12121 cactattcat tggtgggaca agcccgaacg taacccgtcg tttcagggca ccgtcgattg 12181 gctttgccgg gttggcacca cggccagcat tcattacgtg gccgaggccg gccgcgtggc 12241 gtgccttgtc tcacctgaca acattgcatg gcacgccggc gacggcggca acggcccggg 12301 caacaacacc agtattggca ttgagtgcaa cccgcgtcag tccgacggcg actacgccac 12361 ggtggccgag ctggtccgtg acctgcgagc cgtctacggc aatttgccga tctatcctca 12421 ccggcactgg acaagcacgg agtgcccagg aacctatgac cttgcccgaa tcaaccgact 12481 agccgcaacg ccggcaccat cacaggagga ccaaatgaac cccgaacaaa accgcatgct 12541 cgttgcgatc tacaacgcac tgttcaacaa gaagagcatg ccgacgccgg ataatcagtc 12601 catcgtcggc ggtgaagcac ttgacgagtt gataaacaac aacgacgtca agatccttgc 12661 caagctcgaa gagatcaacc gaaagctcta aggaccatga cagccaagcc aactcccaaa 12721 gtagcggccg tcggtgtcag cggcgctctc acagtcctaa tcgtctgggt agcgggacta 12781 tgcggcattg atatgccggc agaagtcgcg gccgctatct ctgtagttgt tacgttcggt 12841 gccggctaca tcaagagcga ggtgacagaa cgtgatggaa agcgaggtga gcacgttgcc 12901 cgatagcggc acatggacgc aacccgaggt tgttcgctcg ttacagcgca ttgagcgcaa 12961 gctagacaac gctgccacga gcggatacgt cgaggcgatc aaagcagacc agttgcgcaa 13021 ggataccgag caagacaagg caattgagtc ggtagaacag aactacaaca aactgttgct 13081 aatggtggtc ggaacggcca taggcagcgc ggcaagctta cttgttgcct tggcgtcagc 13141 cctacctaaa taggccagaa tgccccgtaa cacctaccaa gggttgcggg gcattctgct 13201 gtcaacgtag agttgacagc atggcaagca aagcgaagtg tgaaacgacc gaatatgccg 13261 gcatgctacg gcgcatgatc cgagcttatg gccggcgcgt cggtgatgct gacgtagagg 13321 acttggccgt gatgctggaa gtgcagcgag agcttgacgc ggctatccaa tcggccgtgg 13381 acagccagcg cgagaaccac ggcaggagtt gggcagacat tgcccgggca acaggcacca 13441 gccgccaagc tgcccagaag aaatacggcg cttagaccct agcgacgttt gggcggacag 13501 ccggccggca acgttcccca aacagctccc caacagcgca cccaagcacg ccggcgaccg 13561 tcacaagctc cgaggcgaac catgggcgct gcccatgcag tttgcggctg accgtggctt 13621 gtgtcagccc gagtcgcgca gcaagatccc attgagtcat gcccagcgcc gatagccggc 13681 gctctatgcg ctttcctatg tcggcatcag tgcttgccgg ccgcgcttgg acccgtgttg 13741 tcatggcgca aggttatgcc aagtaatgac attaatcacc acttgtttac gtcaatcgag 13801 tactcattta tcgacccccg ctacttggtt ggcgtgtcgt aacttgtcgt ttcatgccag 13861 aggggtatag cgttcagggc atgacagagc aatcaaacga aacgacagaa cttgtcgaag 13921 ctgaccgagc ggccgagctg ctgggagtca gcaagcgaac gttggacagg tatcaagccg 13981 ccggcctact gaccccaatc cggcccattc agggcaaggg cgcaatacgc cggtttgacg 14041 ctcaggacgt gcagcggctt gctgtagctc aggacgtgca gccatgagca ttgagtcaat 14101 ggccgtagtg cttcaccaca gccaagccgg cggcactgac aagctggtgc tgctgggtat 14161 cgccaaccac gacggcgacg gcggatcttg gccgagcgtg gcaaccctgg cacgctatgc 14221 caacgttgag ccgcgtgcag tcaaggcgtg catcaagcgt cttgttgata ggggcgaggt 14281 tgagcgcgag cggcaagccg gcggcacccg taacatgccc gactacaccc ggccgaacct 14341 ttaccacata aaagtagtct gtccgccgga atgtgaccgt agcgcacagc accgaatcaa 14401 cagaaaagac ccggtgtcgt caacgacacc cggtgtcggc cagatacccc cgggggggta 14461 cgttccagat acccccgggg gggtacgctc aacgacaccc aaaccatcac taaaccatcc 14521 ctctaacact gataaatcac ctagctctag tacctcacca gcggtcaacg gcaaattgcc 14581 gtgctggaac tgtggggaac acgtcattgc gaacaccacc aagccgaaac gctactgcca 14641 gtcatgcagc tcacgcggac ttgataaccc gttgatccca tgcaagaaat gcggctcagt 14701 gcgaaagcgc agctacccgg gagaacagga gttcgactgt ggatgcgttt gagccatacg 14761 aaacctactc catggccgtc ctatggaaca tgagcgcaca agccgcccat gatgccccgg 14821 tagacggcga cgcgctggca agaagcaatt ggcagactct cagcattcaa cggcagtggg 14881 aacggctcac gccgtgccaa gttcagaaaa tccgagacaa ccatcacgaa gtagacaggg 14941 atagcagatc atgaccggac tcagccaagc caccgttgac caagccaagc agacggcggt 15001 caacatggag gccattgcca actcatatca gaagaccatg gagtggaacc gtcaggagta 15061 catcaaagac gccaccacgg ataagtggcc tcagtacata gccgcactca gtgaatggca 15121 gatacatgcc caacgcgcta ccacggcgcg gctcatgtat gaagccattg cccatgccta 15181 ccacctaacc gaggtatggg cacggtgcaa ggcgctgccc accgctgccg atgctgcccg 15241 gtgaatgggg aggacgtgag gcccaagacc tcaccaagct gtgcatggct acctatgggt 15301 ggacgtgcca cctatgcaag ctgcccatca ggcagggtga gcagagcgca gaccacctat 15361 tgccacgcaa gtacggcgga agcaatgacc tatcgaacct cagaccagct caccgcaaat 15421 gtaactatgc acgcggtgcc aagttgctgt cagatccaag agcaaggcca acagacaaca 15481 ccgcgttttt taagtagggg cggctctgga caccccgcgc cacc //