LOCUS MN082624 26061 bp DNA linear PHG 07-JUL-2019 DEFINITION Bacillus phage VioletteMad, complete genome. ACCESSION MN082624 VERSION MN082624.1 KEYWORDS . SOURCE Bacillus phage VioletteMad ORGANISM Bacillus phage VioletteMad Viruses; Caudovirales; Podoviridae. REFERENCE 1 (bases 1 to 26061) AUTHORS Williams,E., Davis,G., Huang,A., Kassa,L., Looney,B., McDonouigh,C., Persinger,R., Pesino,J. and Temple,L. TITLE Direct Submission JOURNAL Submitted (19-JUN-2019) Center for the Study of Biological Complexity, Virginia Commonwealth University, 1015 W. Floyd Ave, Richmond, VA 23284, USA FEATURES Location/Qualifiers source 1..26061 /organism="Bacillus phage VioletteMad" /mol_type="genomic DNA" /isolation_source="soil" /db_xref="taxon:2023952" /lab_host="Bacillus thuringiensis kurstaki" /country="USA: Harrisonburg, VA" /lat_lon="38.428986 N 78.865173 W" /collection_date="28-Aug-2016" /collected_by="E. Williams" /identified_by="E. Williams" gene complement(205..345) /locus_tag="VIOLETTEMAD_1" CDS complement(205..345) /locus_tag="VIOLETTEMAD_1" /note="AJ added.; SD 189 is poor, but has some coding potential (covers all) and six 1:1 blast matches, plus a 1:1 to a bacterial sequence.; NKF" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QDH50326.1" /translation="MIGFSTVKRADKWWVLNMPCNTVRDDFTRKPCESNLTVKLVARF SS" gene complement(414..674) /locus_tag="VIOLETTEMAD_2" CDS complement(414..674) /locus_tag="VIOLETTEMAD_2" /note="SSC: 414 - 674; Predicted by: G&G; Length: 261; Longest ORF?: yes; Gap/Overlap: none; SD score: 588, highest; Blast: 7 1:1 matches, top hit with Claudi, 98% query & 100% identity coverage; Function: N/A; Function source: N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QDH50283.1" /translation="MNYTVEENKKRQVNVDVKYIDKKNVKLHLNIYKLGKLKDIKELT FSNKEMCMIHVENLIKTTEYNLDGFANDFYQGKERFFITLRK" gene complement(671..1021) /locus_tag="VIOLETTEMAD_3" CDS complement(671..1021) /locus_tag="VIOLETTEMAD_3" /note="SSC: 671 - 1021; Predicted by: G&G; Length: 351; Longest ORF?: no; gap/overlap: none; SD score: 399. not highest; Blast: 1 1:1 match, top hit with Claudi, 99% query & 100% identity coverage; Function: N/A; Function source: N/A; Note: blastp does detect a conserved domain but it is a 'protein of unknown function'" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QDH50284.1" /translation="MGFICSYSNCKESVYDMPTLYCKKHYEEYLQNVTEDILNKSNHK EIIKPNHYHEGVNVDPIWYGKEHFTHEEMKGFYKMNVIKYVTRADKKNGLEDLIKAKN YLEMLIENVENNDE" gene complement(1021..1518) /locus_tag="VIOLETTEMAD_4" CDS complement(1021..1518) /locus_tag="VIOLETTEMAD_4" /note="SSC: 1021 - 1518; Predicted by: G&G; Length: 498; Longest ORF?: yes; gap/overlap: overlap of 3; SD score: 777, highest; Blast: 3 1:1 matches, top hit with Claudi, 99% query & 100% identity coverage; Function: dUTPase; Function source: HHpred, multiple hits with value less than 10^-05, top hit has 99.92 probability and evalue of 2.7e-27" /codon_start=1 /transl_table=11 /product="dUTPase" /protein_id="QDH50285.1" /translation="MMEDMLKVQKKVDGDISHKIETSFKETLHLRKIAFKVELSELAN EVGFFKYWKTSHKQNKERVLDEWADCLAFLNSIVISQGYEDYVISYYDKRLDIDIETT TFIRQDYLFRMMNKNDMDSIDDVLSQYFYLYMFGKNLGYSIDEMNSAYNTKSMVNIIR AKEGY" gene complement(1515..1793) /locus_tag="VIOLETTEMAD_5" CDS complement(1515..1793) /locus_tag="VIOLETTEMAD_5" /note="SSC: 1515 - 1793; Length: 279; Predicted by: G&G; Longest ORF?: no; Gap/Overlap: gap of 2; SD score: 546, highest; Blast: 2 1:1 matches, 98% query and 100% identity coverage, top match with Claudi; Function: glutaredoxin; Function Source: HHpred, multiple hits with evalue less than 10^-05, top hit having 99.18 probability and 2.7e-13 evalue" /codon_start=1 /transl_table=11 /product="glutaredoxin" /protein_id="QDH50286.1" /translation="MNIIMYTKNNCPNCMRAEFMFSACPTPVNIEKRNVDENESHKSY LTEVFDSNTLPTFVYESDVLEFGEWKKKINVLRGFDENLGKLQEVLGL" gene complement(1795..2265) /locus_tag="VIOLETTEMAD_6" CDS complement(1795..2265) /locus_tag="VIOLETTEMAD_6" /note="SSC: 1795 - 2265; Length: 471; Predicted by: G&G; Gap/Overlap: gap of 12; Longest ORF?: yes; SD score: 399, not highest; Blast: 3 1:1 matches, top match with KonjoTrouble, 99% query and 100% identity coverage; Function: N/A; Function Source: N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QDH50287.1" /translation="MENFEQEIMELKADIKELKDYIEEKTIKYNDMKNKYESYMMSPK EELGREIDQLKSENEVLKDELKKMNVFVENFESKNNQLKLKDEQITDIKDNFKDYRES KGDYEKTYNIEYTKFDGMLEKVVDGEYKSIKATEIPKFIENNNFEKITIVKVGE" gene complement(2277..2948) /locus_tag="VIOLETTEMAD_7" CDS complement(2277..2948) /locus_tag="VIOLETTEMAD_7" /note="SSC: 2277 - 2948; Predicted by: G&G; Length: 672; SD Score: 819, highest; gap/overlap: 135; CP: covers all CP; Longest ORF?: yes; Blast: 1 1:1 match with KonjoTrouble, 99% query & 100% identical coverage; Function: N/A; Function Source: N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QDH50288.1" /translation="MIEYCDGIVKLYNVETPNNKKGSWVNEEDFLRVVEELNNTKSRL ERVVESKDITINQLRRAIEEKDVEIEKLKGLYKTDVNYYIDENKRLFKELKEMTKHKD KTFMKVLELETELFSIKGEETWTLKYIYDVDGVVKEYEQNGMFKEDAEELIGMDSDNW NHWSLTKEERPDKDKVIEGLLIRCETDEELIKELESRNENLANELYKLKNDQRLKIKP CDRRA" gene complement(3083..3679) /locus_tag="VIOLETTEMAD_8" CDS complement(3083..3679) /locus_tag="VIOLETTEMAD_8" /note="SSC: 3083 - 3679; Predicted by: G&G; Length: 597; SD Score: 882, highest; gap/overlap: 97; CP: covers all CP; Longest ORF?: yes; Blast: 7 1:1 matches, top match with KonjoTrouble, 99% query & 100% identity coverage; Function: N/A; Function Source: N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QDH50289.1" /translation="MALQTITLDFTGKIAGSTTQNPNIAYFNDVEFTQLQYDQIFGSE LRVATTDVLKFEFPVPTEAQINLQSVGIYYKSKLDSGENELRSSDKGVYNDDTTMYKQ KLLEIYGVDIQDQTIKFNITTTGDYGVYEVKCDVTYDDAGLNIYGGQYQYNDTGGVVV TVPIQNKKLEEITAELESRPVNSVTNVTFAQVGTELVP" gene complement(3776..3925) /locus_tag="VIOLETTEMAD_9" CDS complement(3776..3925) /locus_tag="VIOLETTEMAD_9" /note="SSC: 3776 - 3925; Predicted by: G&G; Length: 150; SD Score: 357, not highest; CP: covers all CP; gap/overlap: overlap of 3; Longest ORF?: yes; Blast: 3 1:1 matches, top hit with KonjoTrouble, 98% query & 100% identity coverage; Function: N/A; Function Source: N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QDH50290.1" /translation="MKRQVRVILKHKKFLEKMLKRNINLRKNFRFNQHYLKIIKELEK WGVKL" gene complement(3922..4062) /locus_tag="VIOLETTEMAD_10" CDS complement(3922..4062) /locus_tag="VIOLETTEMAD_10" /note="SSC: 3922 - 4062; Predicted by: G&G; Length: 141; SD Score: 481, not highest; CP: covers all CP; gap/overlap: gap of 4; Longest ORF?: yes; Blast: 3 1:1 matches, top match Claudi, 97% query & 100% identity coverage; Function: N/A; Function Source: N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QDH50291.1" /translation="MWVVIHKEKMSIFSGYKSLDLAERACENLNEVKRNKYYVEFVEV RG" gene complement(4066..4392) /locus_tag="VIOLETTEMAD_11" CDS complement(4066..4392) /locus_tag="VIOLETTEMAD_11" /note="SSC: 4066 - 4392; Predicted by: G&G; Length: 327; SD Score: 588; CP: covers all CP; gap/overlap: overlap of 3; Longest ORF?: yes; Blast: 1:1 matches with all hits, top match Claudi, 99% query & 100% identical coverage; Function: N/A; Function Source: N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QDH50292.1" /translation="MIEKDVYKFKNKIKVGIMYSIYTHLGLINDVDFSLLDDDTLDAY LQGAIRSTTNCKRENEWEQWYVGLMFENKGFRGYIICKDKNKLKEYLVDAMIDYRYPK YSIVEV" gene complement(4389..4511) /locus_tag="VIOLETTEMAD_12" CDS complement(4389..4511) /locus_tag="VIOLETTEMAD_12" /note="SSC: 4389 - 4511; Predicted by: G&G; Length: 123; SD Score: 441, not highest; CP: covers all CP; gap/overlap: overlap of 3; Longest ORF?: yes; Blast: 1:1 match with Claudi, 97% query & 100% identical coverage; Function: N/A; Function Source: N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QDH50293.1" /translation="MIEKEIKEFMEFLSKKYKLSINEIREKMIKQCLNRRDRLK" gene complement(4508..4636) /locus_tag="VIOLETTEMAD_13" CDS complement(4508..4636) /locus_tag="VIOLETTEMAD_13" /note="SSC: 4508 - 4636; Predicted by: G&G; Length: 129; SD Score: 588, not highest; CP: covers all CP; gap/overlap: gap of 16; Longest ORF?: yes; Blast: 3 1:1 matches, top hit KonjoTrouble, 97% query & 100% identity coverage; Function: N/A; Function Source: N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QDH50294.1" /translation="MKEIEEYKNKIIQEAIDSMDKKVIEELIKAKEEYDKNKEVER" gene complement(4652..6724) /locus_tag="VIOLETTEMAD_14" CDS complement(4652..6724) /locus_tag="VIOLETTEMAD_14" /note="SSC: 4652 - 6724; Predicted by: G&G; Length: 2073; SD Score: 714, highest; CP: covers all CP; gap/overlap:; Longest ORF?: no; Blast: gap of 16; Function: DNA polymerase; Function Source: HHpred, multiple hits with e-value less than 10^-05, top hit has 100 probability and 3.2e-54 evalue" /codon_start=1 /transl_table=11 /product="DNA polymerase" /protein_id="QDH50295.1" /translation="MVKKKRKKATIKTYACDFETNTENWLHDKNPKENQRVKEVNPDL WKGREAWRQHTGGDQAFVWCWGGTEIREDMSFKGELDNFLFGNSMKDFMDWLLDGSKN VWFHNLKFDGSYITVQLLRMGYKFTFERNPAIGEFTGLIDGKKMWFDITICKEGPRGG RQFINIKDSLKKVPMGLRACAMAFGLDVFKDDLDYDEIRLPYQPITPADYKYLKKDVE ITAKIIHYQVFQSGLNKTTIGSDALNEFKSTIGGNKGFQNIFPVLDYETDSFIRKSYF GGITQVKPGHEGELVGEGSVYDIVSMYPFVQYYKLLPYGMPVPYQGNYYDMSEEEREG YPLYIQKVSFSFLVKDDHLPTIQLKKQNVEFNYNDMDDVMKFNGREFQKTSFGEIVTM YLTNVQWEQIQRQYWLDDIIYHEGFMFEGKVGIFKDHIDKWLKVKKEANDCGNFALKN LSKLMLNSPYGKFGTNVLKQNVEPFLQDESGSLGFKLQNDDEIRHMTPEEYKQYEIDN ASDPVYTAYASFVTAYARVELVNAVMTCYDRFRYCDTDSIHIVGTEIPEGLKDKIGGD LGQWEPESEFKYAKFHRAKTYCEMIFGKKVKKKDRWGDMVTVVKHISKKEWEGLPEEE RCLDKNLKCAGMQKAIADTVDFEEFEIGLKIDPYNPAKEKWRNVGKLMPSQVAGGTLL RLRKFSLN" gene complement(6740..7657) /locus_tag="VIOLETTEMAD_15" CDS complement(6740..7657) /locus_tag="VIOLETTEMAD_15" /note="SSC: 6740 - 7657; Predicted by: G&G; Length: 918; SD Score: 714, highest; CP: covers all CP; gap/overlap: gap of 40; Longest ORF?: yes; Blast: 3 1:1 hits, top match with KonjoTrouble, 99% query & 100% identical coverage; Function: terminal protein; Function Source: blastp detects conserved domain, top hit evalue: 2.73e-17" /codon_start=1 /transl_table=11 /product="terminal protein" /protein_id="QDH50296.1" /translation="MVKRRSKKQPKFTIRDVDVNEFNRLQNNAKAMIRSRKKKYGVDI SGEIDLKTSISDFKTRKGFNAWKEAMSKLRYRADLQIKKVGDTVASQKQINQSQREVN VMKRKLQKVDGEYQNKYKVRFNEKQLLELTLQTNVARDMEIARQKQLESIPRFNKKGQ QIKDVRKDKTGGTVIVRDKFDPTILDNNQRVKIREYNLKQVSDPERYSRREQQLKENQ LTRLKQAFGSDAEDVIRYIEDMNSQEFNNFYFMFMDSSLGFNEFDSEQYIGQSAETDD QLLGAIEAIRTDIQRYDKNREKYKMLMKY" gene complement(7697..7945) /locus_tag="VIOLETTEMAD_16" CDS complement(7697..7945) /locus_tag="VIOLETTEMAD_16" /note="SSC: 7697 - 7945; Predicted by: G&G; Length: 249; SD Score: 462, highest; CP: covers all CP; gap/overlap: gap of 86; Longest ORF?: no; Blast: 5 1:1 matches, top hit with Claudi, 98% query & 100% identical coverage; Function: N/A; Function Source: N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QDH50297.1" /translation="MGVAIDMTGKKYNRLTCIEKLEERQKGHIMWRFKCDCGKVITAR GGDVRQGKTVSCGCRTIEIGKNNKGKSNEERRKKALSK" gene complement(8031..8387) /locus_tag="VIOLETTEMAD_17" CDS complement(8031..8387) /locus_tag="VIOLETTEMAD_17" /note="SSC: 8031 - 8387; Predicted by: G&G; Length: 357; SD Score: 441. not highest; CP: covers all CP; gap/overlap: gap of 13; Longest ORF?: yes; Blast: 3 1:1 matches, top hit with KonjoTrouble, 99% query & 100% identity coverage; Function: N/A; Function Source: N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QDH50298.1" /translation="MNYTGKRKKVYKFIKNGEKIAEGTIDEMSEQLGITKHSIWNKVS RSVNGRLKRVTYEMVEVSNSVNEYVLMINDKFIGKGSLKRLSEISHFSQEYLTKISNG NYIPKRIRIEIFKKVG" gene complement(8400..8549) /locus_tag="VIOLETTEMAD_18" CDS complement(8400..8549) /locus_tag="VIOLETTEMAD_18" /note="SSC: 8400 - 8549; Predicted by: G&G; Length: 150; SD Score: 609, highest; CP: covers all CP; gap/overlap: gap of 12; Longest ORF?: yes; Blast:1 1:1 match with Claudi, 98% query & 100% identical coverage; Function: N/A; Function Source: N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QDH50299.1" /translation="MEELTSKSLKDIKEIIENTDRWCDDDLTLTYVKIKAIIELLEER GIFNN" gene complement(8561..8722) /locus_tag="VIOLETTEMAD_19" CDS complement(8561..8722) /locus_tag="VIOLETTEMAD_19" /note="SSC: 8561 - 8722; Predicted by: G&G; Length: 162; SD Score: 588; CP: covers all CP; gap/overlap: gpa of 58; Longest ORF?: yes; Blast: 1 1:1 match with Claudi, 98% query & 100% identity coverage; Function: N/A; Function Source: N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QDH50300.1" /translation="MDKEELIIKLKIISEKLRCGLIDQKTFDKEIEYLIRYKWKENKS FTKNSNYYN" gene complement(8780..9151) /locus_tag="VIOLETTEMAD_20" CDS complement(8780..9151) /locus_tag="VIOLETTEMAD_20" /note="SSC: 8780 - 9151; Predicted by: G&G; Length: 372; SD Score: 286; CP: covers all CP; gap/overlap: gap of 866; Longest ORF?: yes; Blast: 3 1:1 matches, top match with KonjoTrouble, 99% query & 100% identity coverage; Function: ssDNA-binding Protein; Function Source: HHpred, multiple hits with evalue less than 10^-05, top hit has 99.88 probability and 1.2e-23 evalue" /codon_start=1 /transl_table=11 /product="ssDNA binding protein" /protein_id="QDH50301.1" /translation="MRKTSNKLKQRKEEIIMNKTILIGRTTKEAELRYTQNGKAVATV DLAVNRDYVAEGQEREADFIRLVIWGKRAETFANFVKKGHQVGVEGALRTRNYDGQDG KKVYVTEVLVDNFTFLEKKSA" gene complement(10017..10232) /locus_tag="VIOLETTEMAD_21" CDS complement(10017..10232) /locus_tag="VIOLETTEMAD_21" /note="SSC: 10017 - 10232; Predicted by: NI, original start was at 10223 but there were no 1:1 blast matches so I changed it to 10232; Length: 216; SD Score: 336, not highest; CP: covers all CP; gap/overlap: gap of 6; Longest ORF?: yes; Blast: 1:1 match with KonjoTrouble, 98% query & 100% identity coverage; Function: N/A; Function Source: N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QDH50302.1" /translation="MGIMEFTIDCAETSYDVYHYNNVLKKYWVNKEERIIKLKSLEDL DQLQKEINEDLIIGKNNSILIYDDYIE" gene complement(10238..10468) /locus_tag="VIOLETTEMAD_22" CDS complement(10238..10468) /locus_tag="VIOLETTEMAD_22" /note="SSC: 10238 - 10468; Predicted by: G&G; Length: 231; SD Score: 567, highest; CP: covers all CP; gap/overlap: gap of 13; Longest ORF?: yes; Blast: 1:1 match with KonjoTrouble only, 98% query & 100% identity coverage; Function: N/A; Function Source: N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QDH50303.1" /translation="MEVKIEVTSILSDIEPYLPVLKEYDYKEGTGTIYLTHLHDIFDL QNKLEKLNLNTALTPSIIVSNDKLIIYDDWIE" gene complement(10481..10669) /locus_tag="VIOLETTEMAD_23" CDS complement(10481..10669) /locus_tag="VIOLETTEMAD_23" /note="SSC: 10481 - 10669; Predicted by: NI, start was originally at 10639, but it didn't have any 1:1 blast matches so I changed it to 10669; Length: 189; SD Score: 357, not highest; gap/overlap: overlap of 7; CP: covers almost all CP; Longest ORF?: no; Blast: 1:1 match with KonjoTrouble, 98% query & 100% identity coverage; Function: N/A; Function Source: N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QDH50304.1" /translation="MINLKTLYDEMLERTYGDILPITIRINDGIPYIKQISKFKRKYL MIKNLKSHTIFFGSMKIK" gene complement(10662..10808) /locus_tag="VIOLETTEMAD_24" CDS complement(10662..10808) /locus_tag="VIOLETTEMAD_24" /note="SSC: 10662 - 10808; Predicted by: G&G; Length: 147; SD Score: 567, highest; CP: covers all CP; gap/overlap: gap of 312; Longest ORF?: yes; Blast: 1:1 match with KonjoTrouble only, 98% query & 100% identity coverage; Function: N/A; Function Source: N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QDH50305.1" /translation="MKIRFQRKDRNKQLMAKQNSKWENKQRDKFLKEQLKKKKANNIL EGSD" gene complement(11120..11200) /locus_tag="VIOLETTEMAD_25" CDS complement(11120..11200) /locus_tag="VIOLETTEMAD_25" /note="TMHMM predicts this to be a transmembrane protein; No blastp results, HHpred has few orange hits (not significant) - but it is called by GeneMark; SSC: 11120 - 11200; Length: 81; SD score: 336; CP - covers most CP; longest ORF?: yes; gap/overlap: gap of 308" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QDH50325.1" /translation="MKKKELLLLSFVWWLAGLSLGFCLWG" gene complement(11212..11292) /locus_tag="VIOLETTEMAD_26" CDS complement(11212..11292) /locus_tag="VIOLETTEMAD_26" /note="Predicted by genemark; SD 609; No coding potential; No sig sim in Blastp" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QDH50327.1" /translation="MKLKIKCKMVFECTSTCEFKWIEIIF" gene complement(11308..11418) /locus_tag="VIOLETTEMAD_27" CDS complement(11308..11418) /locus_tag="VIOLETTEMAD_27" /note="Predicted by genemark; SD 609; Covers coding potential; No sig sim in Blastp" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QDH50328.1" /translation="MLNIFGSIVLFFILTFFFVFVVCCACEVAFRLFNKD" gene complement(11508..11615) /locus_tag="VIOLETTEMAD_28" CDS complement(11508..11615) /locus_tag="VIOLETTEMAD_28" /note="NI inserted this bc it was predicted by GeneMark and has one blast match to Claudi; SSC: 11508 - 11615; Predicted by: G&G; Length: 108; SD Score: 399, not highest; gap/overlap: 26; CP: covers all CP; Longest ORF?: yes; Blast: no 1:1 matches, only existing match with Claudi, 97% query & 100% identity match; Function: N/A; Function Source: N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QDH50324.1" /translation="MYLVSKTKYWFMWVFSMLGISALIFILIRCFDLLF" gene complement(11641..11778) /locus_tag="VIOLETTEMAD_29" CDS complement(11641..11778) /locus_tag="VIOLETTEMAD_29" /note="SSC: 11641 - 11778; Predicted by: G&G; Length: 138; SD Score: 609, highest; CP: covers all CP; gap/overlap: gap of 89; Longest ORF?: yes; Blast: 1:1 match with Claudi and 2 others, 98% query & 100% identical coverage; Function: N/A; Function Source: N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QDH50306.1" /translation="MKMSVIEMMVKTLLLQGKNEDEIFYSLCDRGVSPVSAQFIIDQY I" gene complement(11867..12175) /locus_tag="VIOLETTEMAD_30" CDS complement(11867..12175) /locus_tag="VIOLETTEMAD_30" /note="SSC: 11867 - 12175; Predicted by: G&G; Length: 309; SD Score: 588; CP: covers all CP; Longest ORF?: yes; gap/overlap: gap of 574; Blast: 8 1:1 matches, top hit with KonjoTrouble, 98% query & 100% identity coverage; Function: ds-DNA binding protein; Function Source: blastp detected conserved domain, e-value: 2.36e-05" /codon_start=1 /transl_table=11 /product="dsDNA binding protein" /protein_id="QDH50307.1" /translation="MAKFITRTIVSTTIVIGELKLGSTEVEVKGKLVEEGKLDLEKAT KVVQKAFKGENVIVLDLVQDEAQYKISVEDFIAHAEKVETVEQLEISEETEEIEQPVA " gene 12749..13051 /locus_tag="VIOLETTEMAD_31" CDS 12749..13051 /locus_tag="VIOLETTEMAD_31" /note="SSC: 12749 - 13051; Predicted by: G&G; Length: 303; SD Score: 399; CP: covers all CP; gap/overlap: gap of 574; Longest ORF?: no; Blast: 1:1 match with KonjoTrouble and 7 others, 98% query & 100% identity coverage; Function: N/A; Function Source: N/A; *originally had 'scaffold protein' as function but I didn't see any signficant HHpred & blastp results so I changed it to hypothetical" /codon_start=1 /transl_table=11 /product="scaffold protein" /protein_id="QDH50308.1" /translation="MDRELLGTLMGEFEAPETVDSRKSEIMLELNEGFSNIIAKQEKL QKDMNAIEKKNLELQKTNSHYANRIATQTLDMSEKKEQLKQKEKKERTLSDALKGL" gene 13110..14438 /locus_tag="VIOLETTEMAD_32" CDS 13110..14438 /locus_tag="VIOLETTEMAD_32" /note="SSC: 13110 - 14438; Predicted by: G&G; Length: 1329; SD Score: 441, not highest; CP: covers all CP; gap/overlap: gap of 51; Longest ORF?: yes; Blast: 1:1 match with Stitch and 4 others, 99% query & 100% identity coverage; Function: major head protein; Function Source: blastp detected a conserved domain, evalue: 1.83e-140" /codon_start=1 /transl_table=11 /product="major capsid protein" /protein_id="QDH50309.1" /translation="MAKINMNDVNGLLGAETTADTLNMIRRELGGAYALAVPEANDRN IGEVGIGINSNPEHRNSFLNQLVDRIGLVVIKHKSMNNPLGKFKKGTMPLGYTIEEIY TDITKAKKFDPTDAESTLYKRELPDTKAFFHQRNREQYYEQTVSQAELKAAFVSYQNL DNFITGIFEALYNSAEVDEYLWMRKLIDQYYEKGFFHHVKVEAPTSQDTARAFVKKMR AYVRKLTLGMGSRKYNHTGVHTRSEMEGLHLFITAETEAEIDVDVLAVAFNMNKTDFL SKVTVIDEFADPAIQAVLVDEDWFMCYDNNIEMTNVYNPKGLYWNYFYHVWQTLSCST LENAVVFSTADAPVPPEPKATISPKTASVKAGESTTFSGSTEGEGEVTDKAYSVTGGT KAGTKIDASTGKLDVDATEEAGADKLTVTFSAKVGGVAVSDTAKVTVTAP" gene 14728..16497 /locus_tag="VIOLETTEMAD_33" CDS 14728..16497 /locus_tag="VIOLETTEMAD_33" /note="SSC: 14728 - 16497; Predicted by: G&G; Length: 1770; SD Score: 546; CP: covers all CP; Longest ORF?: yes; gap/overlap: gap of 290; Blast: 1:1 match with KonjoTrouble and 8 others, 99% query & 100% identity coverage; Function: tail protein; Function Source: blastp detects conserved domain, e-value: 0; S. Caruso knows this functional annotation, so AJ added 'knob'." /codon_start=1 /transl_table=11 /product="major tail protein" /protein_id="QDH50310.1" /translation="MAVVPLSGSNVFFKKGVPFSNDNKHTRWFDNINDQFSYFSSRPT VHAMDEVKFVENNGKNYIGADAGIDSLRDVNYIMFQNTQYNNKWFYAFVTQLKRKTSS MTEVYFEIDVLQTWRFEMTMLSSFVVREHCPLWNPDGTPVINTIDEGLNYGTEYETVN VHHHIPNSGIRFLVIACKKAVHGTNKDKVLPSLVGVGQPFSYYVVPFVDKDVVVNATI QGESHRMSTLIDTLASLYKDDKMTNNIVTMFITEQTGLKYTTAENEAGYGINFSSDGQ VVEYAESDGAKMVYVSDCKQFSTKNTFIGSKYDGYRSVKESKLLMYPYTVLTLDDMQG NRRDFKNEYISRPDITLTAKGSLGTSNKVSYSIAGYNMDINSPMHQFMLDEWGIQNIN PNDVSIITELISAYIQGNKNQLHNQVDQITLNGTANVGQSLLGAGTSLASGSYAGVAS SGIGAVKGAGNSVLQLQAISAKIDDIMNVPPTINKMGTNTSYDVGNGFNGVFLIKKQI KPEYQKKLEDFFKLYGYKKNEVKVPNLHTRQNWNYVQTKDVNIIGDFNTEDLNELKAI FDGGITLWHTNDVGNYTLSNEVI" gene 16500..17453 /locus_tag="VIOLETTEMAD_34" CDS 16500..17453 /locus_tag="VIOLETTEMAD_34" /note="SSC: 16500 - 17453; Predicted by: G&G; Length: 954; SD Score: 798, highest; CP: covers all CP; Longest ORF?: yes; gap/overlap: gap of 3; Blast: 1:1 match with KonjoTrouble and one other, 99% query & 100% identity coverage; Function: upper collar protein; Function Source: blastp detects a conserved domain, evalue: 5.16e-88" /codon_start=1 /transl_table=11 /product="upper collar protein" /protein_id="QDH50311.1" /translation="MFNNLQLYMNPNQIQEKAGNFYYWHYAKQLSQLTFQLFEWENLP ESVDPRFLEMMLHTRGYVGFYKDNNDSFVATDGAAGTKLNRYWQPTIFKTVSTNPDDE KISYDIFNYGDDPELIKQNRHGLVIWNNDLHIPTMDSVIMFAKKLANASEIIDINLNA QKTPVLVTAEDSNKFSLMQIYNQYEGNTPVIVANKHFDPKTISVHKTDAPYVVDKIND QKNAYWSEFYTMLGIQNVPIDKKERLTSAEATSGNERDRASENIMLKNRKDFVERAKL LYPGELEDLDVKMRTDILQMYMDNEGFEIDDEQGGEVDGSL" gene 17440..18507 /locus_tag="VIOLETTEMAD_35" CDS 17440..18507 /locus_tag="VIOLETTEMAD_35" /note="SSC: 17440 - 18507; Predicted by: G&G; Length: 1068; SD Score: 525; CP: covers all CP; Longest ORF?: yes; gap/overlap: overlap of 13; Blast: 1:1 match with KonjoTrouble and 6 others, 99% query & 100% identity coverage; Function: lower collar protein; Function Source: blastp detects a conserved domain, e-value: 3.65e-42" /codon_start=1 /transl_table=11 /product="lower collar protein" /protein_id="QDH50312.1" /translation="MALYSIELRRYIDHFTQYERPQPPIKKRIEVGQPHLFDFDYPFF DESKRKDFERKWIRRFYMREVGFETIELFKFHLENWMNERMPYYNQRFKSELIEFDPL LNTRMDRSKDKNIDGTRKDNIDTTGNKKGTTGLKTSENGEFETHTANDGETKNSGTGK LTSDGTNDTTTERNVEDTGTKKGNNQNVHDGTSFARTLDEDTPDGRLDITTEDGKGII RYASKINEVTGKDHANDEIHIDESTTKNTTDNTTDKVKTHDQSNSETTASGESHDVGK ATGTNSSTGTKDGTSNEDVTGNSKLDQTTNQIMLEKENYVGKIGVETYSEMLQKYRET FLSIETEIYNECEAKLFMLVY" gene 18522..20675 /locus_tag="VIOLETTEMAD_36" CDS 18522..20675 /locus_tag="VIOLETTEMAD_36" /note="SSC: 18522 - 20675; Predicted by: NI, original start was at 18600 but the blast matches weren't 1:1, changed start to 18522; Length: 2154; SD Score: 567; CP: covers almost all CP; gap/overlap: none; Longest ORF?: yes; Blast: 1:1 match with KonjoTrouble only, 99% query & 100% identical coverage; Function: pre-neck appendage protein; Function Source: HHpred - multiple hits with evalue under 10^-05, top hit has 99.91 probablity & evalue 5.3e-24" /codon_start=1 /transl_table=11 /product="pre-neck appendage protein" /protein_id="QDH50313.1" /translation="MSNVSKVSTLPISPYRRYLPSAFDESMNIYEQLITCIEYVNNLG ISFNELVDWLDKVVLQQNERLDEQDKKIDMLRDEWHIFEEYVINTLLKKKVVEVLKEW LEDGTLAEIINNDVMNMKVDKTGTVYVKDFKKLEDETDDTGRVKRAFEELKKDENSNL VFEPIKYVVSEGFEVPTNKVIRGYKGKTVIDGSNIETATTLYQKGLFHVKGALAEPIG LAQSVAQGDSKIVAPACDALLIGDLLIITSDESYAEGAPPSSRRGEIVTVKSFDGSNI ELQGEVYFSYDQTKNARVQCMRGMKNVTIKDLDIIMGGKGKGHNGVIVENAQRIIIKN VFIDGAEDCGVVMTNCYNSHVYKSDIINNTSPGGTIGTSGYGVAFLSSKECSARDNFF RNSRHAIAGGGFIPAFACDITGNKAVDCPQYAYDCHEPCFFWNFSNNSATSCVGGFTI RGQHTRVVGNTITSTFANSILVESFTPVDNQKGTLIANNTIQYSKLNGIYCDGTNAIQ TDLTIIGNKVSDVKFAAINVYNLTNAIIEGNTTINDYENGIRVLGRATGYRSKKLVIK GNTVYKSRFSNIRVAGVDNVIISGNNVETNTKDGIELFGAKEFIVDGNLVKDCEFYGI RSEESTNGSYANNYLKTIRGENSDGLRIIKGGKIVMNGNTVIDPKRFGIYTTDTLYTV ITSNNVYDCPSDGVKIDGPIKTHINQNNLTKAIDA" gene 20675..21913 /locus_tag="VIOLETTEMAD_37" CDS 20675..21913 /locus_tag="VIOLETTEMAD_37" /note="SSC: 20675 - 21913; Predicted by: G&G; Length: 1239; SD Score: 525, not highest; CP: covers all CP; Longest ORF?: yes; gap/overlap: none; Blast: 1:1 match with KonjoTrouble and 4 others, 99% query & 100% identity coverage; Function: lytic transglycosylace; Function Source: blastp detects conserved domain, e-value: 4.43e-60" /codon_start=1 /transl_table=11 /product="morphogenesis protein" /protein_id="QDH50314.1" /translation="MKEGQKSVGANGKQNSMFPMDVMYITQGESGDFSHSKAKAIDYI HLTKSGVRTRRAWYYAPADMTVVNAGSAGTMWATDDEVNTPTGTKRMCYMFWHDNNHS AYPVGTKRKQGEKCGQTGTAGFATGDHLHIEVMNGAVFDKSNAVHNWDAFFINDTEIV VDFGYAWKTTDDQTGIDNGTCTPSVPVGNGTLQLNEKVNDKVRSYENQMRAECTAQGI PDATIPLLALMMVESGGAGGDPMQSSESAGLPMNTIKDPAASIRQGVKHFKESMETSK QYDCDIWTIFQQYNYGIGYARFIGSRGKKHTLELSMEYSRTVVAPSLGNTTGRTTPYV NEVSVALGVPWRYVNGGNFHYASMIQYYTTGDGAINSCGGDNTGETDKENKKLNDYIA QLLSNQVSGWNFKRNRYVEG" gene 21948..22097 /locus_tag="VIOLETTEMAD_38" CDS 21948..22097 /locus_tag="VIOLETTEMAD_38" /note="SSC: 21948 - 22097; Predicted by: G&G; Length: 150; SD Score: 504; CP: covers all CP; gap/overlap: gap of 2; Longest ORF?: yes; Blast: 1:1 match with Claudi only, 98% query & 100% identity coverage; Function: N/A; Function Source: N/A; Contains one predicted transmembrane helix, amino acids 7-29.; CDD 10^-3 hit to YvrJ family protein from Bacillus sp.; 93% probability match to Holin BhlA in pfam.; The Phage_holin_BhlA family is a family of holin-like proteins from both bacteriophages and bacterial chromosomes. In bacteriophage, holins are small membrane proteins that accumulate and oligomerise to form non-specific lesions in the cytoplasmic membrane allowing the release of the second protein, endolysins, to access the peptidoglycan. Most holins share common structural features: two or three transmembrane domains separated by a beta-turn, a short hydrophilic N-terminus, a highly charged C-terminus and a dual translational start motif. The BhlA holin of Bacillus is found to be toxic to the host cell where the site of action of is on the cell membrane and causes bacterial death by cell membrane disruption" /codon_start=1 /transl_table=11 /product="holin" /protein_id="QDH50315.1" /translation="MEQLVPFISQVGFPIFVAVFMMTKVTSALDSVKDAVNNLTIAIE KMEER" gene 22099..22896 /locus_tag="VIOLETTEMAD_39" CDS 22099..22896 /locus_tag="VIOLETTEMAD_39" /note="SSC: 22099 - 22896; Predicted by: G&G; Length: 798; SD Score: 714; CP: covers all CP; gap/overlap: gap of 2; Longest ORF?: yes; Blast: 1:1 match with KonjoTrouble & 8 others - 99% query & 100% identity coverage; Function: endolysin; Function Source: HHpred - multiple hits with e-value less than 10^-05, top hit has probability 100% & 1.3e-37 evalue" /codon_start=1 /transl_table=11 /product="endolysin" /protein_id="QDH50316.1" /translation="MGNIVDISKWNGDINWDTAKPYIDFIIARVQDGSNYRDPRYNGY VADMKRKGIPFGSYAFCRFVSINDAKKEAQDFWERGDKSSTVWVADVEVKTMDDMRAG TQAFIDELRRLGAKKVGLYVGHHMYEPFGMSQVQSDFVWIPRYGGSKPKYPCDIWQYT ETGHTPGIGKCDLNQLIGSKNLAYFTGQDDQTPKGYQYVRSGGFGSDLIGEVSTKMNE LGTKGRVILNPSEGLAYIQTDVMPNGELDKITWWMDQRGWWYEYIQG" gene 23107..24207 /locus_tag="VIOLETTEMAD_40" CDS 23107..24207 /locus_tag="VIOLETTEMAD_40" /note="SSC: 23107 - 24207; Predicted by: G&G; Length: 1101; SD Score: 567; CP: covers all CP; gap/overlap: gap of 80; Longest ORF?: no; Blast: 1:1 match with KonjoTrouble and 7 others, 99% query & 100% identity coverage; Function: ATPase encapsidation protein; Function Source: blastp detects conserved domain, evalue - 9.4e-135" /codon_start=1 /transl_table=11 /product="ATPase encapsidation protein" /protein_id="QDH50317.1" /translation="MENLTLFEFEKEQNEEKKDKIENKLEKKKIEDLYYNPQQLLSYN RIMNFTIASRGIGKTYSMKKYCIKRFLKTGAQFIYLRMYKTELKKVDQLFNDVSQEFP DTKFETKGKEFYINGQLAGFAVPLSAWQSFKGNSFPNVETILFDEFIREKDNVGYPPN CVEALLNIIDTVIRNRDNFRCVCLSNSVSVVNPWFLYFNILPEPDKETGKFKRFYKYK HAVLEIPDGRDFKEERIKTRFGAMISELEYGRMSLDNEFTHDVDTFIMKRAKTSVHFC NVVYKGFTMGMWVDTKSDFMFLSQDYDPSSKKTFALTKDDMSENRILVNNYRNEYYLD KIVRAFKKGLLMFDNQIVRQTSYDMFKKMGVQ" gene complement(24272..24418) /locus_tag="VIOLETTEMAD_41" CDS complement(24272..24418) /locus_tag="VIOLETTEMAD_41" /note="SSC: 24272 - 24418; Predicted by: G&G; Length: 147; SD Score: 756; CP: covers all CP; gap/overlap: overlap of 3; Longest ORF?: yes; Blast: 1:1 match with KonjoTrouble and 1 other, 97% query & 100% identity coverage; Function: N/A; Function Source: N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QDH50318.1" /translation="MKNKIIEVNGKLKIEKVTKIETSSLYGKFNNKKNYDICSMYPTI KPSN" gene complement(24415..24600) /locus_tag="VIOLETTEMAD_42" CDS complement(24415..24600) /locus_tag="VIOLETTEMAD_42" /note="SSC: 24415 - 24600; Predicted by: G&G; Length: 186; SD Score: 546; CP: covers almost all CP; gap/overlap: gap of 2; Longest ORF?: no; Blast: 1:1 match with QCM11 and one other, 98% query & 100% identity coverage; Function: N/A; Function Source: N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QDH50319.1" /translation="MITIEYNSYGSLDIKSQTFKGDNCFDDFLYWFFKNDIRDYKFTK GKEYILGEIKQKGINNK" gene complement(24602..24895) /locus_tag="VIOLETTEMAD_43" CDS complement(24602..24895) /locus_tag="VIOLETTEMAD_43" /note="SSC: 24602 - 24895; Predicted by: G&G; Length: 294; SD Score: 609; CP: covers all CP; gap/overlap: none; Longest ORF?: no; Blast: 1:1 match with KonjoTrouble & 6 others, 98% query & 100% identity coverage; Function: N/A; Function Source: N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QDH50320.1" /translation="MEKITRNDSRFLPKFSINRKVNYINDSSLSNLIKRADEMCEKYG LEYESRIYKVTELYSYDEMNNTAVRFKSGKTRYKGAKSEHKEFYEVKLIKVGA" gene complement(24895..25038) /locus_tag="VIOLETTEMAD_44" CDS complement(24895..25038) /locus_tag="VIOLETTEMAD_44" /note="SSC: 24895 - 25038; Predicted by: G&G; Length: 144; SD Score: 483; CP: covers all CP; gap/overlap: gap of 3; Longest ORF?: no; Blast: 1:1 match with KonjoTrouble & one other, 97% query & 100% identity coverage; Function: N/A; Function Source: N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QDH50321.1" /translation="MSYSDYTRALLEISRQQREGIIDLELSLKLEKFCKELHNIWKEK VNK" gene complement(25041..25328) /locus_tag="VIOLETTEMAD_45" CDS complement(25041..25328) /locus_tag="VIOLETTEMAD_45" /note="SSC: 25041 - 25328; Predicted by: G&G; Length: 288; SD Score: 504; CP: covers all CP; gap/overlap: gap of 1; Longest ORF?:yes; Blast: 1:1 match with KonjoTrouble & 6 others, 98% query & 100% identity coverage; Function: N/A; Function Source: N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QDH50322.1" /translation="MNNNNYDQFDVFIQNELHKGNKLKNLELAEKRAMRKAYCKANNI EFEELPTMQENLSSGSLKIIVFVMWLFIAGTIISAFIGEGNFLTNFMKAWF" gene complement(25329..25763) /locus_tag="VIOLETTEMAD_46" CDS complement(25329..25763) /locus_tag="VIOLETTEMAD_46" /note="SSC: 25329 - 25763; Predicted by: G&G; Length: 435; SD Score: 504; CP: covers all CP; gap/overlap: N/A; Longest ORF?: yes; Blast: 1:1 match with KonjoTrouble & 4 others, 99% query & 100% identical coverage; Function: early protein; Function Source: HHpred, one significant hit, probability 100 & 7.7e-35" /codon_start=1 /transl_table=11 /product="early protein" /protein_id="QDH50323.1" /translation="MSIYTMGIVVLVFIAIVGMILGSVKETSNNVKATTSQVMQMNEL ADKKEELKEIKKLIKIKQQELENLVYATEQAKRRTVTNLTVEQEEAIDKYESMGIRLP IDIIEELSYSNNVTYNSAMSFIEAQRKVWKAQFSITMTKGMM" ORIGIN 1 aaatgtaaag gggtacactt ttttcggatt tttcatccat aaaatgtaac tcatgtcaat 61 aaatctttta ttttttattt tttaaatttc taagaaatta cttgttttat ttaactaaaa 121 atgttaatac tatattttat taattttgtc aagtttatat ttataaaaat aatgagttta 181 tttcactcat tttatctttc ttatttaaga tgaaaacctt gctactaatt tcaccgttag 241 gttactttca caaggctttc tagtaaaatc atcacggact gtattacaag gcatattcag 301 tacccaccat ttgtcggcac gtttcaccgt ggaaaacccg atcaaaatat aatatggaag 361 tcctaatgtc ttactctttc aagtataaca tagtcttcca tatttgtcaa gtattatttt 421 ctaagagtaa taaagaacct ttcttttcct tgataaaaat cattagcaaa cccatctaaa 481 ttatattctg tggtcttgat aagattctca acatgtatca tacacatttc tttattagaa 541 aaagttaatt cttttatatc tttaagctta cctaatttat aaatatttaa atgtaatttt 601 acattctttt tatctatgta tttaacatca acattaactt gtctcttttt attttcttca 661 actgtataat tcattcgtca ttattctcca cattttctat taacatttca aggtaatttt 721 tagctttaat taaatcctct aaaccattct ttttatcagc tctagtaaca tatttaataa 781 cattcatctt ataaaaaccc ttcatttcct catgtgtaaa gtgttcttta ccataccata 841 ttggatcaac attaactcct tcgtgataat gattaggttt tattatttct ttatgattag 901 acttgtttaa aatgtcttca gttacatttt gcaaatattc ttcataatgt tttttacaat 961 ataaagttgg catatcataa acactttctt tacaattaga ataggaacat ataaatccca 1021 tcaatatccc tccttagctc taattatatt aaccatactt tttgtattat aagcagaatt 1081 catttcatca atactataac ctaaattctt accaaacata taaagataga aatactgact 1141 caaaacatca tcaatagaat ccatatcatt tttattcatc attctaaata agtaatcttg 1201 tcttataaaa gtggttgttt caatatcgat atccaatctc ttatcataat aacttataac 1261 ataatcttca tatccttgac taattacaat actatttaag aaagccaaac aatctgccca 1321 ctcatctaaa acacgttctt tattttgttt atgagatgtt ttccaatatt taaagaatcc 1381 aacttcatta gctaactcac ttaattcaac tttaaaagct atttttctaa gatgtaaagt 1441 ttctttaaaa gaagtttcta ttttatgtga aatatcgcca tcaacttttt tctgaacttt 1501 caacatatct tccatcataa tcccaaaacc tcctgtaatt tacctaaatt ttcatcaaat 1561 cctcttaaaa cattaatttt ctttttccat tcaccaaatt caagaacatc agattcatac 1621 acaaatgtag gaagagtatt agaatcaaat acttcggtca aatatgattt atgtgattca 1681 ttttcatcaa catttctctt ctcaatattc acaggtgtag gacaagcgga aaacataaat 1741 tccgctcgca tacaattagg acaattattc ttcgtataca taataatatt catcttattc 1801 accaaccttc acaatagtaa ttttttcaaa gttattattt tcaataaact taggtatttc 1861 tgtcgcctta atagatttat attcaccatc aacaactttc tctaacatac catcaaactt 1921 agtatattca atattataag tcttttcata atcaccttta gattctctat aatctttaaa 1981 attatctttg atatcagtta tctgctcatc ttttaatttc aattggttgt ttttactttc 2041 aaagttctca acaaatacat tcatcttttt taactcatct tttaaaactt cattctcact 2101 tttaagttga tcaatctctc ttcccaattc ttctttagga gacatcatat atgattcata 2161 cttattcttc atatcattat atttaattgt cttctcctca atataatctt tcaattcctt 2221 aatatccgct tttaattcca taatttcttg ttcaaagttt tccatttcaa ttcctcctaa 2281 gctcttctat cacacggttt tatttttaat ctttgatcat ttttaagttt atataattca 2341 ttagctagat tttcatttct actctccaac tcttttatca attcctcatc agtttcacat 2401 ctaatcaata atccttctat aactttatct ttatcaggtc tttcttcttt tgttaaactc 2461 cagtggttcc aattatctga atccatacct attaattctt cagcatcttc tttaaacatt 2521 ccattttgct catattcttt cacaacacca tcaacatcat atatgtattt caatgtccaa 2581 gtttcttcac ctttaatact aaataattct gtttctaatt ctaaaacctt cataaatgtt 2641 ttatctttat gttttgtcat ttcttttaat tctttaaata aacgtttatt ttcatctata 2701 taataattaa catcagtttt atataaccct tttaattttt ctatctccac atctttttct 2761 tcaattgctc gtctcaattg attaatagta atatctttag attcaacaac tctttcaagt 2821 cttgatttag tattatttaa ttcctccaca acccttaaaa aatcttcttc attaacccat 2881 gaaccttttt tattattagg tgtttcaaca ttatataatt taacaattcc atcacagtat 2941 tctatcattt ctccattacc tccatataaa aagagaacac tacagtagct gttccctcta 3001 tttaattgta ttaaggtttt gaatttaatc ttttccttaa atttataata acactatttt 3061 catcttttga caagttttat ttttaaggaa ctaattccgt tccaacttga gcaaatgtaa 3121 cattagtaac actatttaca ggtcttgatt ctaactccgc tgtgatctct tccaacttct 3181 tattctgaat aggaacagta actacaacac caccagtatc gttatattga tattgaccac 3241 cgtaaatatt taatcctgcg tcatcataag taacatcaca tttaacttca tagacaccat 3301 aatctcctgt tgtcgttata ttaaatttaa tagtttgatc ttgaatatca acaccataaa 3361 tttctaataa cttttgttta tacattgtgg tatcatcatt ataaacaccc ttatcagatg 3421 atcttaattc attctcccca ctatctaatt tagatttata atagatacca acggattgaa 3481 gatttatttg agcttcagtt ggaacaggaa attcaaattt caaaacatca gttgtagcaa 3541 ctcgcaattc actaccaaat atctgatcat actgtaattg agtaaactca acatcattaa 3601 agtatgcaat attaggattt tgagttgttg atcctgctat cttccctgta aaatctaaag 3661 taattgtttg taaagccatt ctacatcacc tcctttcgaa atctatatcc tagataaatc 3721 acagttcctg taaccatact accaatacaa taactagcta cacaatataa caccgtcata 3781 gtttaacacc ccatttttct aattctttta taatttttaa ataatgttga ttaaatctaa 3841 aattttttct taggttaata tttcttttta acattttttc aaggaatttc ttatgtttaa 3901 gaataactct aacttgtctt ttcatcctct tacctccaca aattccacat agtatttatt 3961 tcttttaact tcattgagat tctcacaagc tctctcagct aaatctaatg atttataacc 4021 actaaatata ctcatctttt ctttatgtat aacaacccac atctcctata cctccacaat 4081 tgaatatttc ggatatctgt aatctatcat agcatcaact aaatattctt tcaacttatt 4141 tttatcttta caaatgatat aacctctaaa tcctttattc tcaaacatta aaccaacata 4201 ccactgttcc cattcatttt ctcttttaca attagttgtt gatcgtatcg ctccttgcaa 4261 atacgcatcc aaagtatcat catctagaag ggaaaaatca acatcgttga ttaatcccaa 4321 atgagtataa atactataca taataccaac ttttattttg ttcttgaact tgtaaacatc 4381 tttttcaatc acttcaatct atcccttcta tttagacatt gtttaatcat tttctctcta 4441 atttcattaa tgcttaattt atatttttta cttaaaaatt ccataaattc tttaatttct 4501 ttctcaatca tctctccacc tctttatttt tatcgtattc ttctttcgct ttaattagtt 4561 cttcaataac tttcttatcc atactgtcaa tagcttcttg tattatttta tttttatatt 4621 cttcaatttc tttcatcttt atctatccct cctaattcaa actaaacttt ctcaatctaa 4681 gcaatgttcc tccagcaact tgagaaggca ttaactttcc aacatttctc cacttctctt 4741 tagctgggtt ataaggatca atcttcaatc caatctcaaa ctcttcaaag tcaaccgtgt 4801 cagctatagc tttttgcata ccagcgcatt ttaaattttt atcaagacat ctttcttctt 4861 caggtaaacc ttcccattct ttcttactga tatgtttaac aacagtaacc atatctcccc 4921 atctatcttt ctttttaacc ttcttaccaa aaatcatttc acaatacgtt ttagctcgat 4981 ggaatttggc atatttaaat tctgattcag gctcccattg acctaaatct ccacctatct 5041 tatcttttaa tccttcagga atttcagttc caacaatatg aattgagtcg gtatcacaat 5101 atctaaaacg atcataacat gtcattacag catttactaa ttctacccta gcataagctg 5161 taacaaaact tgcataagct gtgtaaacag gatcggatgc attatcaatt tcatattgtt 5221 tatattcttc aggtgtcatg tgtctaattt catcatcatt ttgtaattta aatcctaaac 5281 ttcctgattc atcttgcaag aatggttcaa cattttgttt aagaacattt gttccaaatt 5341 taccataagg cgaatttaac ataagtttac tgagattttt caaggcaaag tttccacaat 5401 catttgcttc cttcttaacc tttaaccact tatcaatatg atctttaaat atccccactt 5461 taccttcaaa cataaaacct tcatgataga taatatcatc taaccaatac tgcctttgaa 5521 tttgttccca ttgcacattc gttaaataca ttgttacaat ttctccaaaa gatgtttttt 5581 ggaactccct accattaaac ttcattacat catccatatc attataatta aactcaacat 5641 tttgtttctt taattgaatt gtaggaagat gatcatcttt aactaagaat gaaaaactta 5701 ctttctgtat gtataaagga tacccttctc tttcttcttc actcatatca taataatttc 5761 cctgatacgg aactggcatc ccataaggca ataacttata atattgaaca aaaggataca 5821 tcgatacaat atcatatact gaaccttctc caacaagctc tccttcatgt cctggtttta 5881 cttgagtaat acctccaaaa taactttttc ttataaagct atctgtttca taatctaaaa 5941 ctgggaatat attttggaaa cctttatttc caccaatagt cgatttgaat tcattcaaag 6001 catctgaacc aattgttgtt ttattcaaac cactttggaa tacttgataa tgaataatct 6061 ttgctgttat ttcaacatcc ttctttagat atttataatc agcaggtgtg ataggttgat 6121 aaggtaatcg tatttcatca tagtctaaat catctttaaa tacatctaaa ccaaaagcca 6181 ttgcgcatgc tcttaatccc attggcactt tctttaaaga atccttaatg tttataaact 6241 gtctaccacc tctagggcct tctttacaaa tggtaatatc aaaccacatc tttttaccgt 6301 caatcaatcc cgtaaattca ccaatagcag gatttctttc aaatgtaaat ttatatccca 6361 ttcttaaaag ttgaactgtt atgtagcttc catcgaattt taaattgtga aaccaaacat 6421 ttttacttcc gtctaataac caatccataa aatctttcat agaattacca aataagaagt 6481 tatccaattc acctttaaaa ctcatatctt ctcttatctc agttccaccc caacaccata 6541 caaaagcttg atctcctcct gtgtgttgtc tccatgcctc tctacctttc cataaatcag 6601 gatttacttc ttttactctt tgattctctt tagggttctt atcatgcaac caattttccg 6661 tatttgtttc aaagtcacaa gcataagtct taatagtggc tttctttctt ttcttcttaa 6721 ccattaccaa tacatccccc taatatttca taagcatttt atacttttct ctatttttat 6781 cataacgttg aatatctgtt ctaatagcct caatagcccc taataattga tcatcagttt 6841 cagcagattg accaatatat tgttctgaat caaactcatt aaatcctaaa ctactatcca 6901 taaacataaa ataaaaatta ttaaattctt gtgaattcat atcttctata tatcttatca 6961 cgtcttcagc atcactacca aatgcttgtt tcaatctggt caactgattt tctttcaatt 7021 gttgttcgcg tctactataa cgttcaggat cacttacttg ttttaaatta tattccctta 7081 tcttaaccct ctgattatta tctaatattg tcggatcaaa tttatccctt acaataacag 7141 ttccacctgt tttatcttta cgaacatctt ttatttgttg acctttttta ttaaatcttg 7201 gtatagattc taattgtttt tgtctagcta tttccatatc cctagcaaca tttgtttgta 7261 atgttaattc taataattgt ttttcattaa atcttacttt atatttattt tgatattctc 7321 catctacttt ttgaagttta cgtttcatca catttacttc tctttgtgat tggttaatct 7381 gtttttgact tgcaacagtg tctccaactt tctttatctg taaatcagct ctatatctca 7441 atttactcat tgcttctttc caagcgttaa aacctttacg tgttttgaaa tcactaatac 7501 ttgtttttaa atcaatttcc ccagaaatgt ccacaccgta ttttttcttt ctagaacgta 7561 tcattgcttt agcgttgttt tgaagtctgt taaattcatt cacatctaca tctctgatag 7621 tgaacttagg ttgtttttta gaacgacgtt ttaccatgtt ttctaatctc ccttgaataa 7681 tctttcaatt cttttattat ttacttaaag ctttctttct cctttcttca tttgatttac 7741 ctttattatt ttttcctatc tcaattgtgc gacaaccaca actaaccgtt ttaccttgtc 7801 taacatcacc ccctctagct gttataacct taccacaatc acatttaaat ctccacataa 7861 tatgaccttt ttgtctttct tcaagttttt caatacaagt taatctatta tatttttttc 7921 ctgtcatatc aattgctaca cccattatta tcaattcctt ttctttaagt tactaatatc 7981 ataacatctt tttgacattt cgtcaataat taaatttaaa aagattggtg ttaaccaacc 8041 ttcttaaata tttctatcct aattcttttt ggaatataat taccatttga tatcttagtt 8101 aaatattctt ggctaaaatg agatatttca cttaaacgtt ttaatgaacc ttttccaata 8161 aatttatcgt ttatcattaa aacatattca ttaacagaat tactaacctc aaccatttca 8221 tatgtaactc tttttaatct tccgttaacc gacctactca ctttattcca aatagagtgt 8281 ttcgttattc caagttgttc actcatttca tctatcgtcc cttcagctat tttttcacca 8341 tttttaatga atttatatac tttctttctt tttcctgtgt agttcattcc tctacctcct 8401 taattattaa aaatacctct ttcttcaagt aattcaatta tcgcttttat tttcacataa 8461 gttaatgtta aatcatcatc acaccatcta tctgtatttt caataatttc ttttatgtct 8521 tttaatgatt tagatgttag ctcttccatc cctctacctc ctaattataa tagttactat 8581 ttttagtgaa acttttattt tctttccatt tatatcttat caaatattca atttctttat 8641 caaatgtttt ctgatcaatt aaaccacatc taagtttttc gcttattatt tttaatttaa 8701 ttattagttc ttctttatcc attatctcgt caatcctcct taaaaataaa aggaagcaat 8761 taagctcccc tctatttaat tatgctgatt tcttctctaa gaaagtaaag ttatcaacta 8821 gtacttcagt tacataaact tttttaccat cttgaccatc gtaattacga gttcgtaatg 8881 caccttcaac accaacttga tgcccttttt taacaaagtt agcaaatgtt tctgcacgtt 8941 taccccaaat aactaatcgg ataaagtctg cttcacgttc ttgaccttca gcaacatagt 9001 ctctatttac agctaaatca actgttgcaa ctgctttacc gttttgtgta taacgtaatt 9061 cagcttcctt agtagtacgt ccgattaaaa ttgttttatt cattataatt tcctcttttc 9121 gttgttttag tttatttgag gttttcctca taacagcatc ctaatgtttc aagtaacagt 9181 atacatgata tttcatttgg atatgtgatt ttaactttaa aggaggtaag ttaacaagaa 9241 cggtaatttt taatattgat ctgttctcta ttcctttaag atgctgttat caagagcgaa 9301 atgtctttcg cttaaccctt gatattatta taacatgaag ttctcactct tacaagctat 9361 aatttttatt tcttttctct tcaatctggt gaatgttaga gaaataattt ttgagtgctt 9421 tcaccctgtc agaaatcaag acatataaag tgtgtggctt tacttgtctt acatgaaggt 9481 tttaaagtct accactgact aacgatgtaa aacattttgt agctgtcgct actgcttagt 9541 taaaatatgt atgacttatg tgaaggaata acccttctaa ggatacgcta aacatatctt 9601 tattccttca agtcaaattt gttaatttag agaagaggct ttcacctctt tcctataagt 9661 tattttgttg cttaattgtg taagttaatt atactatgta gtctttccta ctgccaacga 9721 aaacttttat ttgatttttt ctttaggttt tctcctgcaa tatacgataa tttattttgg 9781 agggttttaa cgttgcccta accttcaacg ggttttatgt ctcttcccta ttttccttca 9841 ggtgtcgggt tgttttacct cgctacgctt gagtggttgt taacgtcaaa tactgacggg 9901 tttaaagttt tccttctttg acatcactgc tttacgttca tggattcaac gggttttaac 9961 gactgtttcc ttgtctgtca tcactatacc atttcttaat catttaagtc aacattttat 10021 tcaatataat catcataaat taaaatagaa ttatttttac ctataattaa atcttcattt 10081 atttcttttt gtagttgatc caagtcttct aaacttttta atttaataat tcgttcttct 10141 ttattaaccc aatacttttt taaaacattg ttataatgat aaacatcata agaagtttca 10201 gcacaatcaa tagtgaattc cattattccc actcctccta ttcaatccaa tcatcataaa 10261 taattagttt atcatttgac actattatag atggtgtcaa agctgtattt aagtttaatt 10321 tctctaactt attttgtaaa tcaaaaatat catgtaaatg tgttagataa atcgttcctg 10381 ttccttcctt ataatcatac tctttcaaaa caggtaaata tggttcaata tcactaagaa 10441 tactagtaac ctctatttta acttccatct ccccacaccc ctatttaatt ttcattgatc 10501 caaaaaatat tgtgtgtgat tttaaatttt taatcattaa gtactttctt ttaaatttag 10561 atatttgttt aatatatgga ataccatcat ttattcttat tgttatgggt aatatgtcac 10621 cataggttct ttctaacatt tcatcatata acgttttcaa attaatcact tccttctaat 10681 atattattcg ctttcttttt ctttaattgt tcttttaaaa atttatctct ttgtttattt 10741 tcccatttac tattttgttt tgccatcagt tgtttattcc tatccttacg ttgaaatcta 10801 attttcattc caaaaccatc tctccttctt tgtctctccg agaatacgcg ctaagcgctt 10861 attcaggaga gacctttttc ttttcccccg aagatcttaa aagattaagg tcaaaagata 10921 aaagatgaaa ataccaatac ataaaccttg tattcctgca aatacacatt ctaaaaaata 10981 tttcattgtt tcacctctta gaagatttta aagacttaag gtcaaaagat aaaagataaa 11041 accaaaacgg gattcttcta ttataatgtt gttcaataaa gatagaatat gatttcattt 11101 taagtcctcc taaaatattc taaccccata aacaaaaacc taaagataaa ccagctaacc 11161 accaaacaaa agacaataac aataattctt tctttttcat tttaagacct cctaaaatat 11221 aatttcaatc catttgaatt cacaagttga agtacattcg aaaaccattt tacacttaat 11281 tttaagtttc attttaagac ctcctagtta atctttatta aataaacgaa aagcaacttc 11341 acaagcacaa caaacaacaa aaacaaagaa gaatgttaat ataaagaata acacaatact 11401 accaaaaata tttaacattt taagacctcc tatttaagat tcaaaccaat acactaaaac 11461 aattaataat ataactattg ataaaagata atatatttta agacctccta aaataataaa 11521 tcaaaacatc taataagaat aaaaattaaa gcagaaattc ctaacatact gaaaacccac 11581 ataaaccaat attttgtttt ggaaactaag tacattttaa gacctcctaa agattaaaga 11641 ttaaatgtat tgatctataa tgaattgtgc tgaaacaggt gaaacacctc tatcacataa 11701 agaataaaat atttcatctt catttttacc ttgcaacaat aaagttttaa ccatcatttc 11761 aattactgac attttcatta ttttacctcc tagttaaaat aagttaattc gtcaattatg 11821 tgtaaaataa aagggtaaag actcaaagcc taaacccttt atttaattat gctacaggtt 11881 gttcaatttc ttctgtttct tctgagattt ctaattgttc aacagtttct actttttcag 11941 cgtgagcaat gaagtcttca acagagattt tgtattgtgc ttcatcttga actaaatcaa 12001 gaacgattac gttttctcct ttgaatgctt tttgaactac ttttgtagcc ttttcaagat 12061 caagtttacc ttcttcaact aatttccctt ttacttctac ttcagttgaa cctaatttta 12121 attctccaat tacgatagtt gttgatacga tagtacgtgt aataaattta gccattttaa 12181 attccttctt tcattttagt ttttatttta atttatttga taacattttt tatttgaaag 12241 aaaagatcaa aagatgaagt tcaaaacctc ttaactttta atgtgtttct ttcgttgaat 12301 taaatataac attttaaaat gataaatgca agggatttta gaaaagaatt ttaaaagatt 12361 ttaaaataat aaaatgaaag atcaaaacca aaagataaaa tcaaaagaga aaaacaagac 12421 aatatcgtga aaccatttat aacaaattaa aaactcataa agatttacta ttaaaatgaa 12481 aaagaaaata aaagtaaaga taaaataaag aatccatttc atttgaatca ctcctttttc 12541 gtgtttcttc tattatatag ggaaaaataa aaagataaaa ccataaatta aaaataagtt 12601 taaatagaaa aaataaataa actttaaata tatcataaaa tatgttattt cgtcaagaaa 12661 taaatgttgc tattttttga ttcatagttt aatatattaa tcaaggggta aatatctcct 12721 aaaatactaa ataatagagg tgaaaataat ggatagagaa ttgttaggaa ctttaatggg 12781 tgaatttgaa gcacctgaaa ctgttgatag tcgtaaatca gaaatcatgt tggaattaaa 12841 tgaaggtttt agcaacatta ttgcgaaaca agaaaaatta caaaaagaca tgaatgctat 12901 tgaaaagaaa aatctagaac ttcaaaaaac aaattcccat tatgcaaacc gtattgcaac 12961 tcaaacactt gatatgagtg aaaaaaaaga acaactgaaa caaaaagaga aaaaagaacg 13021 aacattaagt gatgcattaa aaggtcttta ggtaataaca ccgctaggtg attacacata 13081 caatttaata gaagaaaaga ggaaaacaaa tggctaaaat taacatgaat gatgttaacg 13141 gtttgcttgg tgctgaaact acagcagaca cattaaacat gattcgtaga gaattaggtg 13201 gagcgtatgc gttagctgtt ccagaagcaa atgatcgaaa tattggtgaa gttggtattg 13261 gtattaactc aaatccagaa caccgcaata gtttcttaaa tcaattagtg gatcgtattg 13321 gtttggttgt tatcaaacat aaatcaatga ataacccgct tggcaaattt aaaaaaggca 13381 caatgccttt aggttacaca atcgaagaaa tttatacaga tatcacgaaa gcgaagaagt 13441 ttgatccaac tgatgctgaa tctactttat acaaacgtga acttcctgac acaaaagcat 13501 tcttccatca acgtaaccgt gaacaatact atgagcaaac agtttcacaa gctgaattaa 13561 aagcagcatt tgtgtcttat cagaatttag ataactttat tactggtatt tttgaagctc 13621 tttataattc tgctgaagtg gacgagtact tatggatgcg taaactgatt gatcagtatt 13681 atgaaaaagg attcttccat catgtaaaag tagaagctcc tacatcacaa gatacagctc 13741 gtgcttttgt taagaaaatg cgtgcttatg ttcgtaaatt aacacttgga atgggttcac 13801 gtaaatacaa ccatactggg gtacatactc gttctgaaat ggaaggatta catctattta 13861 ttacagcaga aacagaagca gaaattgatg ttgatgtatt agcagttgct ttcaacatga 13921 acaaaactga tttcttatct aaagtaactg taattgatga atttgctgat ccagcaattc 13981 aagcagttct tgttgatgaa gattggttca tgtgttatga caacaatatt gaaatgacaa 14041 atgtttacaa tccaaaaggt ctatactgga actacttcta ccatgtatgg caaacacttt 14101 cttgttcaac tcttgaaaat gctgttgtat tctctacggc agatgctcca gttcctcctg 14161 aaccaaaagc aacgatttca ccaaaaacag ctagtgtaaa agctggtgaa tctacaacat 14221 tctcaggttc tactgaaggt gaaggcgaag taacagataa agcatattct gtaactggtg 14281 gaacaaaagc aggaactaaa attgatgctt caactggaaa attagatgtt gatgctactg 14341 aagaagctgg ggcagataaa ttaactgtaa cattctcagc taaagtaggc ggagtagcgg 14401 tatctgatac agctaaagta acagtcactg caccctaagc cacccaaacc aacaattgat 14461 cctgtttctg taggtgaaaa tattcatgtt acaggaacag gaattcctaa ttactggatt 14521 catttaaaac atgaacaagt aagtggtgga ggaacaagtg atgtcttccg aattgaaggt 14581 gatgggtcat acagaagttt aaatacttta atacatgttt cagttggaga tattgttaaa 14641 gtagctcaat ctcctcatga atttgatgat gaggaattat ggtcagattt tgaaatatat 14701 gaagtaaaat agaaaggagc gtctaaaatg gctgttgtac cgttaagtgg aagtaacgtc 14761 ttttttaaaa aaggcgttcc tttttctaat gataataaac acacaagatg gtttgataat 14821 ataaacgatc aatttagtta ttttagcagt agacctaccg ttcatgctat ggacgaagtg 14881 aagtttgtgg agaataacgg taaaaactac attggagcag atgctggaat tgattcttta 14941 cgagatgtaa actatattat gttccaaaat actcaatata ataacaaatg gttttatgct 15001 tttgtaacac agttaaaaag aaaaacaagt tcgatgacag aagtatattt cgaaattgat 15061 gtgctgcaaa catggcgctt tgaaatgaca atgttatcat catttgttgt acgagaacat 15121 tgcccattat ggaatcctga tggaacacct gttattaata cgattgatga ggggttaaat 15181 tatggaactg aatatgaaac agtaaatgtt catcatcata ttccgaatag cggtattcgt 15241 tttcttgtta ttgcttgtaa aaaggcggta catggaacaa ataaagataa ggttttacct 15301 agtcttgttg gtgttggaca accttttagt tattatgttg ttccgtttgt tgataaagac 15361 gttgttgtga atgctaccat acaaggtgaa tcacatcgaa tgtcaacatt aatagataca 15421 ctcgcgtctc tttataaaga tgataaaatg acaaataaca ttgttaccat gtttataaca 15481 gaacaaacag gtttgaaata cacaacggca gaaaatgaag ctggatacgg aatcaatttt 15541 agtagtgatg gtcaggtagt agaatatgct gaatctgatg gagctaagat ggtttatgta 15601 agtgactgta aacagttttc tacaaaaaac acttttattg ggtctaaata tgatggatat 15661 agaagtgtaa aagaaagtaa gctattgatg tatccgtaca ctgttttaac attagatgat 15721 atgcaaggta accgtagaga ttttaaaaat gaatatatat ctagacctga tattacatta 15781 acagctaaag gatcgctagg tacaagcaat aaagtttcct atagtattgc tggatataat 15841 atggatataa atagtcctat gcatcaattt atgttggatg aatggggaat acaaaatatt 15901 aatccgaatg atgtatctat cataactgaa ttaatttcag cttatattca aggtaataag 15961 aatcaacttc ataatcaagt tgatcaaatt acattgaatg gcacagcgaa tgttggtcaa 16021 agtttattgg gtgcaggaac gtcactagca tctggaagtt atgcgggtgt agcatcttct 16081 ggtattggag ctgttaaagg agctggcaat agtgttcttc aattgcaagc tatttcggct 16141 aaaattgatg atattatgaa tgttccacca actattaata aaatgggaac gaacacaagt 16201 tatgatgttg gtaatggttt taatggtgta ttcttgataa agaaacagat taaacctgaa 16261 tatcaaaaga aactagaaga tttctttaaa ttatatggtt ataaaaagaa tgaagttaaa 16321 gttcctaatc ttcacactag acagaactgg aattatgtgc aaacaaaaga tgtaaatata 16381 attggtgatt ttaacacgga agatttgaac gagttgaaag ctatatttga tggtggaatt 16441 acattgtggc atacgaacga tgttggaaat tatactttaa gtaatgaggt gatatagaca 16501 tgtttaataa tttacaatta tatatgaatc caaatcaaat acaggaaaaa gcaggaaatt 16561 tctactattg gcattatgca aaacaattaa gccaattaac tttccaatta tttgagtggg 16621 aaaacttacc agaatctgtt gatcctcgtt tcttagaaat gatgttacac acacgtggtt 16681 atgttggatt ttataaagat aacaatgata gcttcgttgc aacagatggt gcagcaggaa 16741 ctaaattaaa tcgttattgg caaccaacaa tatttaaaac tgtttctact aatccagatg 16801 atgaaaaaat tagttatgac atttttaatt atggtgatga tcctgaatta attaaacaaa 16861 atagacatgg acttgttatt tggaataatg atttacatat tccaacaatg gatagtgtta 16921 ttatgtttgc gaaaaagtta gcaaatgcgt ctgagattat tgacatcaac ttaaatgctc 16981 agaagacacc tgtattagtt acagcagagg attcaaataa gttttcatta atgcagattt 17041 ataatcaata tgaagggaat acacctgtta ttgtagctaa taaacacttt gatccgaaaa 17101 cgattagtgt tcataaaact gatgctcctt atgttgttga taaaattaat gaccagaaga 17161 atgcatattg gtctgaattc tatacaatgt tggggattca gaatgttcct attgataaga 17221 aggaaagatt gacaagtgca gaggcaacat ctggtaatga aagagataga gcatctgaaa 17281 atatcatgct taagaatcgt aaagattttg ttgaaagagc aaaactttta tatccgggag 17341 aattagaaga tttagatgta aaaatgagaa cagatattct tcaaatgtat atggataatg 17401 aagggtttga aatagatgat gaacaagggg gtgaagtaga tggctcttta tagtattgaa 17461 ttaagaagat atatagatca ctttactcaa tatgaaagac ctcaaccccc tattaagaaa 17521 agaattgaag ttggtcaacc ccacctattt gattttgatt atcctttctt tgatgaaagt 17581 aagagaaaag attttgaaag aaaatggatt agaagattct atatgagaga agttggattt 17641 gagactattg agttattcaa atttcattta gaaaactgga tgaatgaaag aatgccttat 17701 tataaccaaa gatttaaaag tgaattaatt gagtttgatc cgctattgaa cacaagaatg 17761 gatcgttcta aagataaaaa tattgatggt actcgtaaag ataacattga tacaactggt 17821 aataagaaag gtacaactgg tcttaaaaca tctgaaaatg gtgaatttga aacccatacc 17881 gcaaatgatg gtgaaactaa gaatagtgga actggaaaat taacttctga tggaacgaat 17941 gatacaacta cagagcgtaa tgttgaagat actggaacta aaaaaggtaa caaccaaaat 18001 gttcatgatg gaactagctt tgctagaaca ttagatgagg atacgccaga cggtagactt 18061 gatattacaa ctgaagatgg taaagggatt atcagatatg cttctaaaat caatgaagtt 18121 acaggaaaag atcatgctaa cgatgaaatt cacattgatg aatcaacaac taagaataca 18181 actgataata ccacagataa agtaaaaaca catgatcaaa gtaatagtga aacaacagct 18241 tctggagaat ctcatgatgt tggtaaagca actggaacca atagttctac tggaactaaa 18301 gacggcactt ctaatgaaga tgttacagga aattctaagc tagatcaaac tactaatcag 18361 atcatgttag aaaaagaaaa ttatgttggt aaaattggag ttgaaacata ctctgaaatg 18421 ttacagaaat atcgtgaaac atttcttagt attgaaactg aaatttacaa tgaatgtgaa 18481 gcaaaactat ttatgttagt atactagaaa ggaatgataa gatgagtaat gttagcaaag 18541 ttagtacgct gccaataagt ccttacagaa gatacttacc aagcgctttt gatgagtcca 18601 tgaatattta tgaacaactt attacatgta ttgaatatgt aaataatctt ggtatatctt 18661 tcaatgaatt agttgattgg ttagataaag ttgtattgca gcagaatgaa agactagatg 18721 aacaagataa aaagattgat atgttacgtg atgagtggca tatttttgaa gaatatgtaa 18781 ttaacaccct tctaaagaaa aaagttgttg aagttcttaa agaatggtta gaagatggga 18841 cgcttgctga aataatcaac aatgatgtaa tgaatatgaa agtcgataaa acaggaactg 18901 tatacgttaa agattttaaa aaattagaag atgaaactga tgatacaggt cgagttaaaa 18961 gagcttttga agagttgaag aaagatgaaa attctaatct tgtgtttgaa ccaattaaat 19021 atgttgtgtc tgaaggtttt gaagttccta ctaataaagt gattagaggt tataaaggaa 19081 aaactgttat tgatggttca aatattgaaa cagctactac attatatcaa aaaggattat 19141 tccatgtaaa gggagctctt gctgaaccta ttggtttagc tcaaagtgtt gctcaaggag 19201 atagtaaaat tgtagctcct gcttgtgatg ctttgttgat tggtgatcta ctaattataa 19261 caagtgatga atcttatgct gaaggcgcac ctcctagttc tcgtagaggt gaaattgtaa 19321 cagtgaagtc atttgatggt tcaaatattg aattacaagg tgaagtttac tttagttatg 19381 atcaaactaa aaacgctaga gttcaatgta tgcgaggaat gaaaaatgta acaattaaag 19441 atttagatat cattatgggt ggtaaaggaa aaggtcataa tggtgttatt gttgagaatg 19501 ctcaacgaat tattattaaa aatgtgttta ttgacggtgc tgaagattgt ggagttgtta 19561 tgacaaactg ttacaactca catgtttata aatcagatat tatcaataat acatcacccg 19621 gtggaacaat tggaacaagt ggttatggtg ttgctttctt atcatcaaaa gaatgttcag 19681 caagagataa cttctttaga aattcacgtc acgctattgc tggaggtggt ttcattcccg 19741 catttgcttg tgatattaca ggaaataaag ctgttgattg tccacaatat gcttatgatt 19801 gtcatgaacc ttgtttcttc tggaacttct ctaataactc agcaacatct tgtgtaggtg 19861 gttttacaat tcgtggtcaa cacactagag ttgtaggaaa cacaattaca agcacatttg 19921 caaatagtat cttagttgaa agtttcacac cagttgataa tcaaaaagga acattaattg 19981 ctaataacac aattcaatat tctaaattaa atggtattta ttgtgatgga acaaatgcaa 20041 ttcaaactga tttaactatc attggaaata aagttagcga tgttaaattt gcagctatta 20101 atgtttataa tttaacgaat gctattatcg agggtaatac aacaataaat gattatgaaa 20161 atggtattcg tgtgttaggt agagcaactg gatatagaag taaaaaacta gttataaaag 20221 gtaatactgt ttataagagt cgtttctcga atattcgtgt tgcaggtgtt gataatgtaa 20281 ttattagcgg aaataatgtt gaaacaaaca cgaaagatgg tattgaatta tttggagcta 20341 aagaatttat tgttgatggt aacttagtta aagattgtga attctatggt attcgatctg 20401 aagaatcaac aaatggctct tatgcaaata actatcttaa gactattcgc ggtgaaaact 20461 cagatgggtt gcgtattatc aaaggtggaa agattgttat gaatggtaac actgttattg 20521 atcctaaacg ttttggtatt tatacaactg atacgcttta tactgttatt acttccaata 20581 atgtgtatga ttgtccttcc gatggtgtta aaattgatgg gccaattaag actcatatta 20641 atcaaaataa cttaacaaag gctattgacg cttaatgaaa gaaggtcaaa aatccgtagg 20701 ggcaaatgga aaacaaaatt ccatgttccc tatggatgtt atgtacatta cacaagggga 20761 atcgggagac ttttcgcata gtaaagctaa ggcgatagat tatatacact taacaaaatc 20821 tggtgtaaga actagaagag cgtggtatta tgcccctgct gatatgactg ttgttaatgc 20881 agggagtgca ggaacaatgt gggctacaga tgatgaagtt aatacaccaa ctggaacaaa 20941 gagaatgtgt tacatgtttt ggcacgataa taatcactca gcatatcctg taggcacaaa 21001 aagaaaacaa ggtgaaaaat gtggtcaaac tggtacagct ggttttgcta caggtgatca 21061 cttgcatatt gaggttatga atggtgcagt ttttgataaa tcaaatgctg ttcacaactg 21121 ggatgctttc tttataaatg atactgaaat tgtggttgat tttggttatg cttggaagac 21181 tacagatgat caaactggta ttgataatgg aacttgtact ccttctgttc ctgttggtaa 21241 tggaacttta caattaaatg aaaaagttaa tgataaggta agaagttatg aaaatcaaat 21301 gagagccgaa tgtacagctc aaggtatacc tgacgcgact attcctttgt tagcactgat 21361 gatggttgaa tctggtggag ctggtggaga cccaatgcag agttctgagt ccgcaggttt 21421 accaatgaat acaattaaag accctgccgc aagcattaga caaggtgtaa aacactttaa 21481 agagtcaatg gaaacttcga aacaatatga ttgtgatatt tggactatat ttcaacagta 21541 taattatggt ataggatatg ctagatttat tggttctaga ggtaaaaaac atactcttga 21601 attatctatg gaatattcaa gaacagttgt tgcacctagt ttaggtaata caacgggtag 21661 aactacacct tacgttaacg aagtttcagt tgctttgggt gttccttgga gatatgtaaa 21721 cggtggaaac tttcattatg ccagtatgat ccaatattac acaactgggg atggtgcaat 21781 taatagttgt ggtggagata atacaggtga aacagataaa gagaataaaa aactaaatga 21841 ttatatagct cagttactaa gtaatcaagt tagcggatgg aattttaaaa gaaatagata 21901 tgttgaagga taaataataa gagaataaga aagtgtgggt gtaatcaatg gagcaattag 21961 tcccatttat tagtcaagtc ggatttccaa tatttgtagc agtatttatg atgacaaaag 22021 taactagtgc tttggatagt gtaaaagacg cagtaaacaa tttaacaata gcgatagaga 22081 aaatggagga aagataatat gggcaatatt gtagatattt ctaaatggaa tggtgatatc 22141 aattgggata ctgctaaacc ttatattgat tttattatag cacgtgttca agatggtagt 22201 aattatcgtg acccacgtta taatggttac gttgctgata tgaaacgtaa aggaatacct 22261 tttggtagct atgctttttg tagattcgta tctattaatg atgctaagaa ggaagctcaa 22321 gatttctggg aacgtggtga taaatcctct accgtttggg tggcggatgt tgaagtgaag 22381 acgatggatg atatgagagc aggaacacaa gcttttattg atgaattacg tcgattagga 22441 gctaagaaag ttggattata tgtaggtcat catatgtatg agccttttgg tatgagtcaa 22501 gttcaatctg actttgtatg gattccgcga tatggtggta gtaaacctaa atatccttgt 22561 gatatttggc aatatactga aactggtcac acaccgggta ttggtaaatg tgatttgaat 22621 caattgattg gtagtaaaaa tctagcttac ttcactggtc aagatgatca aacacctaaa 22681 ggatatcaat atgttagaag tggtggattt ggtagtgatt taataggtga agtttcaact 22741 aaaatgaatg agcttggaac aaaaggtaga gttattttaa atccgagtga aggtcttgcg 22801 tatattcaaa ctgatgtaat gccaaatgga gaattagata aaataacttg gtggatggat 22861 caacgtggtt ggtggtatga gtatatacag ggttagggga aataaggggt cataattttt 22921 gacccttccc tatttaaaag taaaaaccta aaacatctaa aaataaaaag cttatggttt 22981 aaaactaaaa ataggaatat cgtagaacct ttaaatataa atatcataaa aataaaatca 23041 ttttaatttt ggatttgacg gaaaaaattt ttggaataaa tataaaaagt gagggatgat 23101 tctgttatgg aaaacttaac attatttgaa tttgaaaaag aacagaatga agaaaagaaa 23161 gataaaatag aaaataagtt agagaaaaag aaaatagaag atttatatta caatcctcaa 23221 caactgttaa gctataacag aatcatgaac ttcacaattg catcgagggg tattggtaaa 23281 acatattcaa tgaagaaata ttgtattaaa agatttttga agacaggtgc acaatttatt 23341 tacttgagaa tgtataaaac agagttgaaa aaagttgacc aactgtttaa tgatgttagt 23401 caggaatttc ctgatactaa atttgaaact aaaggtaaag agttttacat aaatggtcag 23461 ctagctgggt ttgctgttcc gttgagtgca tggcaaagct ttaaaggtaa ttcattccct 23521 aatgttgaaa caatattatt tgatgagttt atacgtgaaa aagataatgt aggatatcca 23581 ccaaactgtg tggaagcact acttaatatt atagatactg taattcgtaa ccgtgataat 23641 ttcagatgtg tttgtttaag taactcggtt tctgttgtta atccatggtt cttatacttt 23701 aatattcttc ctgagccaga taaagaaact ggtaagttta agaggtttta taaatacaaa 23761 cacgctgtac ttgaaatacc tgatggtaga gacttcaaag aggaaagaat taaaacgaga 23821 tttggtgcta tgattagtga gttagaatat ggtcgaatga gtttagataa tgaatttact 23881 catgatgtcg atactttcat tatgaagaga gctaagactt ctgttcactt ctgtaatgtt 23941 gtttataaag gttttacgat gggaatgtgg gttgatacta aaagcgactt tatgtttctg 24001 tcacaagatt atgacccaag ttctaagaag acttttgcat tgactaaaga tgacatgagt 24061 gaaaatagaa tacttgttaa taattataga aatgaatact acttagataa gattgtgaga 24121 gctttcaaaa aaggactgtt gatgtttgat aatcagattg ttagacaaac tagttatgat 24181 atgtttaaaa agatgggtgt acaataatta aagacttcaa aataaagtta aaagaattaa 24241 aagatttaaa agacaaagaa aaaagccttc cttaattgga gggcttaatt gttggataca 24301 ttgaacaaat atcgtaattc tttttgttgt taaatttacc atataatgaa gatgtttcta 24361 tcttagttac tttttctatt tttagtttcc cattaacttc tataatttta tttttcattt 24421 gttgtttatc cccttttgtt ttatttcacc tagaatgtat tctttacctt ttgtgaattt 24481 ataatctctt atatcatttt tgaagaacca gtatagaaaa tcatcgaaac aattatctcc 24541 tttgaatgtt tgtgatttta tatctaatga accgtatgag ttgtattcta ttgttatcat 24601 gttaagcacc aacctttatt aatttaactt cgtagaattc tttgtgttct gatttagcac 24661 ctttgtaacg tgttttacct gatttaaaac ggacagcggt attgttcatt tcatcataac 24721 tgtataattc tgttacttta tatattctag attcatactc taaaccatat ttctcacaca 24781 tttcatctgc tcttttaatt aagtttgata gtgaagaatc atttatgtaa tttacttttc 24841 tgttaataga aaacttaggt aagaatcttg aatcgtttct agttatcttt tccattactt 24901 gtttaccttt tctttccaaa tgttatgtaa ttctttacaa aatttttcta gttttaatga 24961 caactctaaa tctattattc cttctcgttg ttgtctactt atttctaata atgctcttgt 25021 gtaatcacta taactcattt ctaaaaccac gctttcatga aatttgttaa gaagtttcct 25081 tcaccaatga atgctgatat tattgttcct gcgatgaata accacattac gaatactata 25141 atctttaaag agccacttga tagattttct tgcattgttg gtagttcttc gaactcaatg 25201 ttgttagctt tacaataagc tttacgcatt gctctttttt cagctagttc taagtttttt 25261 aatttgtttc ctttatgtag ttcgttttga ataaatacat caaattgatc atagttatta 25321 ttgttcattt acatcatccc cttagtcatt gtaattgaga attgagcttt ccatacttta 25381 cgttgagctt caatgaaaga cattgctgaa ttgtatgtta cattgttaga ataagataat 25441 tcttcaatga tgtcaatagg taatctgatt cccattgatt catatttatc aattgcttct 25501 tcttgttcta cagttaagtt tgttactgtt ctgcgtttag cttgttcggt tgcatatact 25561 aagttttcta attcttgttg tttgattttg ataagttttt taatttcttt aagttcttct 25621 ttcttatcag ctaattcatt catttgcatt acttgtgaag ttgttgcttt tacattattt 25681 gatgtttctt ttactgaccc taaaatcatt cctacgattg ctataaagac taatactacg 25741 attcccattg tgtaaattga cattttgttt aatccccttt atgattgttt tagttgtttt 25801 cttaacctat aattattata tcatttatgt gttaaatcgt caatagattt atttaattct 25861 tttagttgtt tttaataact aattaatcaa cacataaata gataaaaata atatataatt 25921 aatataacat atttaattta aaatatcaag gatatatcgt caactaatat aaataaatat 25981 caacattcaa aaccataaat ataaatattt gtcaagataa aaatggggga aacttaaaat 26041 aaagtgtacc catttacatt t //