##gff-version 3
#!gff-spec-version 1.21
#!processor NCBI annotwriter
#!genome-build ASM686960v1
#!genome-build-accession NCBI_Assembly:GCA_006869605.1
##sequence-region MN082624.1 1 26061
##species https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=2023952
MN082624.1	Genbank	region	1	26061	.	+	.	ID=MN082624.1:1..26061;Dbxref=taxon:2023952;collected-by=E. Williams;collection-date=28-Aug-2016;country=USA: Harrisonburg%2C VA;gbkey=Src;identified-by=E. Williams;isolation-source=soil;lab-host=Bacillus thuringiensis kurstaki;lat-lon=38.428986 N 78.865173 W;mol_type=genomic DNA
MN082624.1	Genbank	gene	205	345	.	-	.	ID=gene-VIOLETTEMAD_1;Name=VIOLETTEMAD_1;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_1
MN082624.1	Genbank	CDS	205	345	.	-	0	ID=cds-QDH50326.1;Parent=gene-VIOLETTEMAD_1;Dbxref=NCBI_GP:QDH50326.1;Name=QDH50326.1;Note=AJ added.~SD 189 is poor%2C but has some coding potential (covers all) and six 1:1 blast matches%2C plus a 1:1 to a bacterial sequence.~NKF;gbkey=CDS;locus_tag=VIOLETTEMAD_1;product=hypothetical protein;protein_id=QDH50326.1;transl_table=11
MN082624.1	Genbank	gene	414	674	.	-	.	ID=gene-VIOLETTEMAD_2;Name=VIOLETTEMAD_2;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_2
MN082624.1	Genbank	CDS	414	674	.	-	0	ID=cds-QDH50283.1;Parent=gene-VIOLETTEMAD_2;Dbxref=NCBI_GP:QDH50283.1;Name=QDH50283.1;Note=SSC: 414 - 674~Predicted by: G%26G~Length: 261~Longest ORF?: yes~Gap/Overlap: none~SD score: 588%2C highest~Blast: 7 1:1 matches%2C top hit with Claudi%2C 98%25 query %26 100%25 identity coverage~Function: N/A~Function source: N/A;gbkey=CDS;locus_tag=VIOLETTEMAD_2;product=hypothetical protein;protein_id=QDH50283.1;transl_table=11
MN082624.1	Genbank	gene	671	1021	.	-	.	ID=gene-VIOLETTEMAD_3;Name=VIOLETTEMAD_3;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_3
MN082624.1	Genbank	CDS	671	1021	.	-	0	ID=cds-QDH50284.1;Parent=gene-VIOLETTEMAD_3;Dbxref=NCBI_GP:QDH50284.1;Name=QDH50284.1;Note=SSC: 671 - 1021~Predicted by: G%26G~Length: 351~Longest ORF?: no~gap/overlap: none~SD score: 399. not highest~Blast: 1 1:1 match%2C top hit with Claudi%2C 99%25 query %26 100%25 identity coverage~Function: N/A~Function source: N/A~Note: blastp does detect a conserved domain but it is a 'protein of unknown function';gbkey=CDS;locus_tag=VIOLETTEMAD_3;product=hypothetical protein;protein_id=QDH50284.1;transl_table=11
MN082624.1	Genbank	gene	1021	1518	.	-	.	ID=gene-VIOLETTEMAD_4;Name=VIOLETTEMAD_4;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_4
MN082624.1	Genbank	CDS	1021	1518	.	-	0	ID=cds-QDH50285.1;Parent=gene-VIOLETTEMAD_4;Dbxref=NCBI_GP:QDH50285.1;Name=QDH50285.1;Note=SSC: 1021 - 1518~Predicted by: G%26G~Length: 498~Longest ORF?: yes~gap/overlap: overlap of 3~SD score: 777%2C highest~Blast: 3 1:1 matches%2C top hit with Claudi%2C 99%25 query %26 100%25 identity coverage~Function: dUTPase~Function source: HHpred%2C multiple hits with value less than 10^-05%2C top hit has 99.92 probability and evalue of 2.7e-27;gbkey=CDS;locus_tag=VIOLETTEMAD_4;product=dUTPase;protein_id=QDH50285.1;transl_table=11
MN082624.1	Genbank	gene	1515	1793	.	-	.	ID=gene-VIOLETTEMAD_5;Name=VIOLETTEMAD_5;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_5
MN082624.1	Genbank	CDS	1515	1793	.	-	0	ID=cds-QDH50286.1;Parent=gene-VIOLETTEMAD_5;Dbxref=NCBI_GP:QDH50286.1;Name=QDH50286.1;Note=SSC: 1515 - 1793~Length: 279~Predicted by: G%26G~Longest ORF?: no~Gap/Overlap: gap of 2~SD score: 546%2C highest~Blast: 2 1:1 matches%2C 98%25 query and 100%25 identity coverage%2C top match with Claudi~Function: glutaredoxin~Function Source: HHpred%2C multiple hits with evalue less than 10^-05%2C top hit having 99.18 probability and 2.7e-13 evalue;gbkey=CDS;locus_tag=VIOLETTEMAD_5;product=glutaredoxin;protein_id=QDH50286.1;transl_table=11
MN082624.1	Genbank	gene	1795	2265	.	-	.	ID=gene-VIOLETTEMAD_6;Name=VIOLETTEMAD_6;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_6
MN082624.1	Genbank	CDS	1795	2265	.	-	0	ID=cds-QDH50287.1;Parent=gene-VIOLETTEMAD_6;Dbxref=NCBI_GP:QDH50287.1;Name=QDH50287.1;Note=SSC: 1795 - 2265~Length: 471~Predicted by: G%26G~Gap/Overlap: gap of 12~Longest ORF?: yes~SD score: 399%2C not highest~Blast: 3 1:1 matches%2C top match with KonjoTrouble%2C 99%25 query and 100%25 identity coverage~Function: N/A~Function Source: N/A;gbkey=CDS;locus_tag=VIOLETTEMAD_6;product=hypothetical protein;protein_id=QDH50287.1;transl_table=11
MN082624.1	Genbank	gene	2277	2948	.	-	.	ID=gene-VIOLETTEMAD_7;Name=VIOLETTEMAD_7;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_7
MN082624.1	Genbank	CDS	2277	2948	.	-	0	ID=cds-QDH50288.1;Parent=gene-VIOLETTEMAD_7;Dbxref=NCBI_GP:QDH50288.1;Name=QDH50288.1;Note=SSC: 2277 - 2948~Predicted by: G%26G~Length: 672~SD Score: 819%2C highest~gap/overlap: 135~CP: covers all CP~Longest ORF?: yes~Blast: 1 1:1 match with KonjoTrouble%2C 99%25 query %26 100%25 identical coverage~Function: N/A~Function Source: N/A;gbkey=CDS;locus_tag=VIOLETTEMAD_7;product=hypothetical protein;protein_id=QDH50288.1;transl_table=11
MN082624.1	Genbank	gene	3083	3679	.	-	.	ID=gene-VIOLETTEMAD_8;Name=VIOLETTEMAD_8;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_8
MN082624.1	Genbank	CDS	3083	3679	.	-	0	ID=cds-QDH50289.1;Parent=gene-VIOLETTEMAD_8;Dbxref=NCBI_GP:QDH50289.1;Name=QDH50289.1;Note=SSC: 3083 - 3679~Predicted by: G%26G~Length: 597~SD Score: 882%2C highest~gap/overlap: 97~CP: covers all CP~Longest ORF?: yes~Blast: 7 1:1 matches%2C top match with KonjoTrouble%2C 99%25 query %26 100%25 identity coverage~Function: N/A~Function Source: N/A;gbkey=CDS;locus_tag=VIOLETTEMAD_8;product=hypothetical protein;protein_id=QDH50289.1;transl_table=11
MN082624.1	Genbank	gene	3776	3925	.	-	.	ID=gene-VIOLETTEMAD_9;Name=VIOLETTEMAD_9;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_9
MN082624.1	Genbank	CDS	3776	3925	.	-	0	ID=cds-QDH50290.1;Parent=gene-VIOLETTEMAD_9;Dbxref=NCBI_GP:QDH50290.1;Name=QDH50290.1;Note=SSC: 3776 - 3925~Predicted by: G%26G~Length: 150~SD Score: 357%2C not highest~CP: covers all CP~gap/overlap: overlap of 3~Longest ORF?: yes~Blast: 3 1:1 matches%2C top hit with KonjoTrouble%2C 98%25 query %26 100%25 identity coverage~Function: N/A~Function Source: N/A;gbkey=CDS;locus_tag=VIOLETTEMAD_9;product=hypothetical protein;protein_id=QDH50290.1;transl_table=11
MN082624.1	Genbank	gene	3922	4062	.	-	.	ID=gene-VIOLETTEMAD_10;Name=VIOLETTEMAD_10;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_10
MN082624.1	Genbank	CDS	3922	4062	.	-	0	ID=cds-QDH50291.1;Parent=gene-VIOLETTEMAD_10;Dbxref=NCBI_GP:QDH50291.1;Name=QDH50291.1;Note=SSC: 3922 - 4062~Predicted by: G%26G~Length: 141~SD Score: 481%2C not highest~CP: covers all CP~gap/overlap: gap of 4~Longest ORF?: yes~Blast: 3 1:1 matches%2C top match Claudi%2C 97%25 query %26 100%25 identity coverage~Function: N/A~Function Source: N/A;gbkey=CDS;locus_tag=VIOLETTEMAD_10;product=hypothetical protein;protein_id=QDH50291.1;transl_table=11
MN082624.1	Genbank	gene	4066	4392	.	-	.	ID=gene-VIOLETTEMAD_11;Name=VIOLETTEMAD_11;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_11
MN082624.1	Genbank	CDS	4066	4392	.	-	0	ID=cds-QDH50292.1;Parent=gene-VIOLETTEMAD_11;Dbxref=NCBI_GP:QDH50292.1;Name=QDH50292.1;Note=SSC: 4066 - 4392~Predicted by: G%26G~Length: 327~SD Score: 588~CP: covers all CP~gap/overlap: overlap of 3~Longest ORF?: yes~Blast: 1:1 matches with all hits%2C top match Claudi%2C 99%25 query %26 100%25 identical coverage~Function: N/A~Function Source: N/A;gbkey=CDS;locus_tag=VIOLETTEMAD_11;product=hypothetical protein;protein_id=QDH50292.1;transl_table=11
MN082624.1	Genbank	gene	4389	4511	.	-	.	ID=gene-VIOLETTEMAD_12;Name=VIOLETTEMAD_12;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_12
MN082624.1	Genbank	CDS	4389	4511	.	-	0	ID=cds-QDH50293.1;Parent=gene-VIOLETTEMAD_12;Dbxref=NCBI_GP:QDH50293.1;Name=QDH50293.1;Note=SSC: 4389 - 4511~Predicted by: G%26G~Length: 123~SD Score: 441%2C not highest~CP: covers all CP~gap/overlap: overlap of 3~Longest ORF?: yes~Blast: 1:1 match with Claudi%2C 97%25 query %26 100%25 identical coverage~Function: N/A~Function Source: N/A;gbkey=CDS;locus_tag=VIOLETTEMAD_12;product=hypothetical protein;protein_id=QDH50293.1;transl_table=11
MN082624.1	Genbank	gene	4508	4636	.	-	.	ID=gene-VIOLETTEMAD_13;Name=VIOLETTEMAD_13;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_13
MN082624.1	Genbank	CDS	4508	4636	.	-	0	ID=cds-QDH50294.1;Parent=gene-VIOLETTEMAD_13;Dbxref=NCBI_GP:QDH50294.1;Name=QDH50294.1;Note=SSC: 4508 - 4636~Predicted by: G%26G~Length: 129~SD Score: 588%2C not highest~CP: covers all CP~gap/overlap: gap of 16~Longest ORF?: yes~Blast: 3 1:1 matches%2C top hit KonjoTrouble%2C 97%25 query %26 100%25 identity coverage~Function: N/A~Function Source: N/A;gbkey=CDS;locus_tag=VIOLETTEMAD_13;product=hypothetical protein;protein_id=QDH50294.1;transl_table=11
MN082624.1	Genbank	gene	4652	6724	.	-	.	ID=gene-VIOLETTEMAD_14;Name=VIOLETTEMAD_14;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_14
MN082624.1	Genbank	CDS	4652	6724	.	-	0	ID=cds-QDH50295.1;Parent=gene-VIOLETTEMAD_14;Dbxref=NCBI_GP:QDH50295.1;Name=QDH50295.1;Note=SSC: 4652 - 6724~Predicted by: G%26G~Length: 2073~SD Score: 714%2C highest~CP: covers all CP~gap/overlap:~Longest ORF?: no~Blast: gap of 16~Function: DNA polymerase~Function Source: HHpred%2C multiple hits with e-value less than 10^-05%2C top hit has 100 probability and 3.2e-54 evalue;gbkey=CDS;locus_tag=VIOLETTEMAD_14;product=DNA polymerase;protein_id=QDH50295.1;transl_table=11
MN082624.1	Genbank	gene	6740	7657	.	-	.	ID=gene-VIOLETTEMAD_15;Name=VIOLETTEMAD_15;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_15
MN082624.1	Genbank	CDS	6740	7657	.	-	0	ID=cds-QDH50296.1;Parent=gene-VIOLETTEMAD_15;Dbxref=NCBI_GP:QDH50296.1;Name=QDH50296.1;Note=SSC: 6740 - 7657~Predicted by: G%26G~Length: 918~SD Score: 714%2C highest~CP: covers all CP~gap/overlap: gap of 40~Longest ORF?: yes~Blast: 3 1:1 hits%2C top match with KonjoTrouble%2C 99%25 query %26 100%25 identical coverage~Function: terminal protein~Function Source: blastp detects conserved domain%2C top hit evalue: 2.73e-17;gbkey=CDS;locus_tag=VIOLETTEMAD_15;product=terminal protein;protein_id=QDH50296.1;transl_table=11
MN082624.1	Genbank	gene	7697	7945	.	-	.	ID=gene-VIOLETTEMAD_16;Name=VIOLETTEMAD_16;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_16
MN082624.1	Genbank	CDS	7697	7945	.	-	0	ID=cds-QDH50297.1;Parent=gene-VIOLETTEMAD_16;Dbxref=NCBI_GP:QDH50297.1;Name=QDH50297.1;Note=SSC: 7697 - 7945~Predicted by: G%26G~Length: 249~SD Score: 462%2C highest~CP: covers all CP~gap/overlap: gap of 86~Longest ORF?: no~Blast: 5 1:1 matches%2C top hit with Claudi%2C 98%25 query %26 100%25 identical coverage~Function: N/A~Function Source: N/A;gbkey=CDS;locus_tag=VIOLETTEMAD_16;product=hypothetical protein;protein_id=QDH50297.1;transl_table=11
MN082624.1	Genbank	gene	8031	8387	.	-	.	ID=gene-VIOLETTEMAD_17;Name=VIOLETTEMAD_17;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_17
MN082624.1	Genbank	CDS	8031	8387	.	-	0	ID=cds-QDH50298.1;Parent=gene-VIOLETTEMAD_17;Dbxref=NCBI_GP:QDH50298.1;Name=QDH50298.1;Note=SSC: 8031 - 8387~Predicted by: G%26G~Length: 357~SD Score: 441. not highest~CP: covers all CP~gap/overlap: gap of 13~Longest ORF?: yes~Blast: 3 1:1 matches%2C top hit with KonjoTrouble%2C 99%25 query %26 100%25 identity coverage~Function: N/A~Function Source: N/A;gbkey=CDS;locus_tag=VIOLETTEMAD_17;product=hypothetical protein;protein_id=QDH50298.1;transl_table=11
MN082624.1	Genbank	gene	8400	8549	.	-	.	ID=gene-VIOLETTEMAD_18;Name=VIOLETTEMAD_18;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_18
MN082624.1	Genbank	CDS	8400	8549	.	-	0	ID=cds-QDH50299.1;Parent=gene-VIOLETTEMAD_18;Dbxref=NCBI_GP:QDH50299.1;Name=QDH50299.1;Note=SSC: 8400 - 8549~Predicted by: G%26G~Length: 150~SD Score: 609%2C highest~CP: covers all CP~gap/overlap: gap of 12~Longest ORF?: yes~Blast:1 1:1 match with Claudi%2C 98%25 query %26 100%25 identical coverage~Function: N/A~Function Source: N/A;gbkey=CDS;locus_tag=VIOLETTEMAD_18;product=hypothetical protein;protein_id=QDH50299.1;transl_table=11
MN082624.1	Genbank	gene	8561	8722	.	-	.	ID=gene-VIOLETTEMAD_19;Name=VIOLETTEMAD_19;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_19
MN082624.1	Genbank	CDS	8561	8722	.	-	0	ID=cds-QDH50300.1;Parent=gene-VIOLETTEMAD_19;Dbxref=NCBI_GP:QDH50300.1;Name=QDH50300.1;Note=SSC: 8561 - 8722~Predicted by: G%26G~Length: 162~SD Score: 588~CP: covers all CP~gap/overlap: gpa of 58~Longest ORF?: yes~Blast: 1 1:1 match with Claudi%2C 98%25 query %26 100%25 identity coverage~Function: N/A~Function Source: N/A;gbkey=CDS;locus_tag=VIOLETTEMAD_19;product=hypothetical protein;protein_id=QDH50300.1;transl_table=11
MN082624.1	Genbank	gene	8780	9151	.	-	.	ID=gene-VIOLETTEMAD_20;Name=VIOLETTEMAD_20;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_20
MN082624.1	Genbank	CDS	8780	9151	.	-	0	ID=cds-QDH50301.1;Parent=gene-VIOLETTEMAD_20;Dbxref=NCBI_GP:QDH50301.1;Name=QDH50301.1;Note=SSC: 8780 - 9151~Predicted by: G%26G~Length: 372~SD Score: 286~CP: covers all CP~gap/overlap: gap of 866~Longest ORF?: yes~Blast: 3 1:1 matches%2C top match with KonjoTrouble%2C 99%25 query %26 100%25 identity coverage~Function: ssDNA-binding Protein~Function Source: HHpred%2C multiple hits with evalue less than 10^-05%2C top hit has 99.88 probability and 1.2e-23 evalue;gbkey=CDS;locus_tag=VIOLETTEMAD_20;product=ssDNA binding protein;protein_id=QDH50301.1;transl_table=11
MN082624.1	Genbank	gene	10017	10232	.	-	.	ID=gene-VIOLETTEMAD_21;Name=VIOLETTEMAD_21;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_21
MN082624.1	Genbank	CDS	10017	10232	.	-	0	ID=cds-QDH50302.1;Parent=gene-VIOLETTEMAD_21;Dbxref=NCBI_GP:QDH50302.1;Name=QDH50302.1;Note=SSC: 10017 - 10232~Predicted by: NI%2C original start was at 10223 but there were no 1:1 blast matches so I changed it to 10232~Length: 216~SD Score: 336%2C not highest~CP: covers all CP~gap/overlap: gap of 6~Longest ORF?: yes~Blast: 1:1 match with KonjoTrouble%2C 98%25 query %26 100%25 identity coverage~Function: N/A~Function Source: N/A;gbkey=CDS;locus_tag=VIOLETTEMAD_21;product=hypothetical protein;protein_id=QDH50302.1;transl_table=11
MN082624.1	Genbank	gene	10238	10468	.	-	.	ID=gene-VIOLETTEMAD_22;Name=VIOLETTEMAD_22;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_22
MN082624.1	Genbank	CDS	10238	10468	.	-	0	ID=cds-QDH50303.1;Parent=gene-VIOLETTEMAD_22;Dbxref=NCBI_GP:QDH50303.1;Name=QDH50303.1;Note=SSC: 10238 - 10468~Predicted by: G%26G~Length: 231~SD Score: 567%2C highest~CP: covers all CP~gap/overlap: gap of 13~Longest ORF?: yes~Blast: 1:1 match with KonjoTrouble only%2C 98%25 query %26 100%25 identity coverage~Function: N/A~Function Source: N/A;gbkey=CDS;locus_tag=VIOLETTEMAD_22;product=hypothetical protein;protein_id=QDH50303.1;transl_table=11
MN082624.1	Genbank	gene	10481	10669	.	-	.	ID=gene-VIOLETTEMAD_23;Name=VIOLETTEMAD_23;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_23
MN082624.1	Genbank	CDS	10481	10669	.	-	0	ID=cds-QDH50304.1;Parent=gene-VIOLETTEMAD_23;Dbxref=NCBI_GP:QDH50304.1;Name=QDH50304.1;Note=SSC: 10481 - 10669~Predicted by: NI%2C start was originally at 10639%2C but it didn't have any 1:1 blast matches so I changed it to 10669~Length: 189~SD Score: 357%2C not highest~gap/overlap: overlap of 7~CP: covers almost all CP~Longest ORF?: no~Blast: 1:1 match with KonjoTrouble%2C 98%25 query %26 100%25 identity coverage~Function: N/A~Function Source: N/A;gbkey=CDS;locus_tag=VIOLETTEMAD_23;product=hypothetical protein;protein_id=QDH50304.1;transl_table=11
MN082624.1	Genbank	gene	10662	10808	.	-	.	ID=gene-VIOLETTEMAD_24;Name=VIOLETTEMAD_24;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_24
MN082624.1	Genbank	CDS	10662	10808	.	-	0	ID=cds-QDH50305.1;Parent=gene-VIOLETTEMAD_24;Dbxref=NCBI_GP:QDH50305.1;Name=QDH50305.1;Note=SSC: 10662 - 10808~Predicted by: G%26G~Length: 147~SD Score: 567%2C highest~CP: covers all CP~gap/overlap: gap of 312~Longest ORF?: yes~Blast: 1:1 match with KonjoTrouble only%2C 98%25 query %26 100%25 identity coverage~Function: N/A~Function Source: N/A;gbkey=CDS;locus_tag=VIOLETTEMAD_24;product=hypothetical protein;protein_id=QDH50305.1;transl_table=11
MN082624.1	Genbank	gene	11120	11200	.	-	.	ID=gene-VIOLETTEMAD_25;Name=VIOLETTEMAD_25;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_25
MN082624.1	Genbank	CDS	11120	11200	.	-	0	ID=cds-QDH50325.1;Parent=gene-VIOLETTEMAD_25;Dbxref=NCBI_GP:QDH50325.1;Name=QDH50325.1;Note=TMHMM predicts this to be a transmembrane protein~No blastp results%2C HHpred has few orange hits (not significant) - but it is called by GeneMark~SSC: 11120 - 11200~Length: 81~SD score: 336~CP - covers most CP~longest ORF?: yes~gap/overlap: gap of 308;gbkey=CDS;locus_tag=VIOLETTEMAD_25;product=hypothetical protein;protein_id=QDH50325.1;transl_table=11
MN082624.1	Genbank	gene	11212	11292	.	-	.	ID=gene-VIOLETTEMAD_26;Name=VIOLETTEMAD_26;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_26
MN082624.1	Genbank	CDS	11212	11292	.	-	0	ID=cds-QDH50327.1;Parent=gene-VIOLETTEMAD_26;Dbxref=NCBI_GP:QDH50327.1;Name=QDH50327.1;Note=Predicted by genemark~SD 609~No coding potential~No sig sim in Blastp;gbkey=CDS;locus_tag=VIOLETTEMAD_26;product=hypothetical protein;protein_id=QDH50327.1;transl_table=11
MN082624.1	Genbank	gene	11308	11418	.	-	.	ID=gene-VIOLETTEMAD_27;Name=VIOLETTEMAD_27;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_27
MN082624.1	Genbank	CDS	11308	11418	.	-	0	ID=cds-QDH50328.1;Parent=gene-VIOLETTEMAD_27;Dbxref=NCBI_GP:QDH50328.1;Name=QDH50328.1;Note=Predicted by genemark~SD 609~Covers coding potential~No sig sim in Blastp;gbkey=CDS;locus_tag=VIOLETTEMAD_27;product=hypothetical protein;protein_id=QDH50328.1;transl_table=11
MN082624.1	Genbank	gene	11508	11615	.	-	.	ID=gene-VIOLETTEMAD_28;Name=VIOLETTEMAD_28;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_28
MN082624.1	Genbank	CDS	11508	11615	.	-	0	ID=cds-QDH50324.1;Parent=gene-VIOLETTEMAD_28;Dbxref=NCBI_GP:QDH50324.1;Name=QDH50324.1;Note=NI inserted this bc it was predicted by GeneMark and has one blast match to Claudi~SSC: 11508 - 11615~Predicted by: G%26G~Length: 108~SD Score: 399%2C not highest~gap/overlap: 26~CP: covers all CP~Longest ORF?: yes~Blast: no 1:1 matches%2C only existing match with Claudi%2C 97%25 query %26 100%25 identity match~Function: N/A~Function Source: N/A;gbkey=CDS;locus_tag=VIOLETTEMAD_28;product=hypothetical protein;protein_id=QDH50324.1;transl_table=11
MN082624.1	Genbank	gene	11641	11778	.	-	.	ID=gene-VIOLETTEMAD_29;Name=VIOLETTEMAD_29;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_29
MN082624.1	Genbank	CDS	11641	11778	.	-	0	ID=cds-QDH50306.1;Parent=gene-VIOLETTEMAD_29;Dbxref=NCBI_GP:QDH50306.1;Name=QDH50306.1;Note=SSC: 11641 - 11778~Predicted by: G%26G~Length: 138~SD Score: 609%2C highest~CP: covers all CP~gap/overlap: gap of 89~Longest ORF?: yes~Blast: 1:1 match with Claudi and 2 others%2C 98%25 query %26 100%25 identical coverage~Function: N/A~Function Source: N/A;gbkey=CDS;locus_tag=VIOLETTEMAD_29;product=hypothetical protein;protein_id=QDH50306.1;transl_table=11
MN082624.1	Genbank	gene	11867	12175	.	-	.	ID=gene-VIOLETTEMAD_30;Name=VIOLETTEMAD_30;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_30
MN082624.1	Genbank	CDS	11867	12175	.	-	0	ID=cds-QDH50307.1;Parent=gene-VIOLETTEMAD_30;Dbxref=NCBI_GP:QDH50307.1;Name=QDH50307.1;Note=SSC: 11867 - 12175~Predicted by: G%26G~Length: 309~SD Score: 588~CP: covers all CP~Longest ORF?: yes~gap/overlap: gap of 574~Blast: 8 1:1 matches%2C top hit with KonjoTrouble%2C 98%25 query %26 100%25 identity coverage~Function: ds-DNA binding protein~Function Source: blastp detected conserved domain%2C e-value: 2.36e-05;gbkey=CDS;locus_tag=VIOLETTEMAD_30;product=dsDNA binding protein;protein_id=QDH50307.1;transl_table=11
MN082624.1	Genbank	gene	12749	13051	.	+	.	ID=gene-VIOLETTEMAD_31;Name=VIOLETTEMAD_31;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_31
MN082624.1	Genbank	CDS	12749	13051	.	+	0	ID=cds-QDH50308.1;Parent=gene-VIOLETTEMAD_31;Dbxref=NCBI_GP:QDH50308.1;Name=QDH50308.1;Note=SSC: 12749 - 13051~Predicted by: G%26G~Length: 303~SD Score: 399~CP: covers all CP~gap/overlap: gap of 574~Longest ORF?: no~Blast: 1:1 match with KonjoTrouble and 7 others%2C 98%25 query %26 100%25 identity coverage~Function: N/A~Function Source: N/A~*originally had 'scaffold protein' as function but I didn't see any signficant HHpred %26 blastp results so I changed it to hypothetical;gbkey=CDS;locus_tag=VIOLETTEMAD_31;product=scaffold protein;protein_id=QDH50308.1;transl_table=11
MN082624.1	Genbank	gene	13110	14438	.	+	.	ID=gene-VIOLETTEMAD_32;Name=VIOLETTEMAD_32;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_32
MN082624.1	Genbank	CDS	13110	14438	.	+	0	ID=cds-QDH50309.1;Parent=gene-VIOLETTEMAD_32;Dbxref=NCBI_GP:QDH50309.1;Name=QDH50309.1;Note=SSC: 13110 - 14438~Predicted by: G%26G~Length: 1329~SD Score: 441%2C not highest~CP: covers all CP~gap/overlap: gap of 51~Longest ORF?: yes~Blast: 1:1 match with Stitch and 4 others%2C 99%25 query %26 100%25 identity coverage~Function: major head protein~Function Source: blastp detected a conserved domain%2C evalue: 1.83e-140;gbkey=CDS;locus_tag=VIOLETTEMAD_32;product=major capsid protein;protein_id=QDH50309.1;transl_table=11
MN082624.1	Genbank	gene	14728	16497	.	+	.	ID=gene-VIOLETTEMAD_33;Name=VIOLETTEMAD_33;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_33
MN082624.1	Genbank	CDS	14728	16497	.	+	0	ID=cds-QDH50310.1;Parent=gene-VIOLETTEMAD_33;Dbxref=NCBI_GP:QDH50310.1;Name=QDH50310.1;Note=SSC: 14728 - 16497~Predicted by: G%26G~Length: 1770~SD Score: 546~CP: covers all CP~Longest ORF?: yes~gap/overlap: gap of 290~Blast: 1:1 match with KonjoTrouble and 8 others%2C 99%25 query %26 100%25 identity coverage~Function: tail protein~Function Source: blastp detects conserved domain%2C e-value: 0~S. Caruso knows this functional annotation%2C so AJ added 'knob'.;gbkey=CDS;locus_tag=VIOLETTEMAD_33;product=major tail protein;protein_id=QDH50310.1;transl_table=11
MN082624.1	Genbank	gene	16500	17453	.	+	.	ID=gene-VIOLETTEMAD_34;Name=VIOLETTEMAD_34;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_34
MN082624.1	Genbank	CDS	16500	17453	.	+	0	ID=cds-QDH50311.1;Parent=gene-VIOLETTEMAD_34;Dbxref=NCBI_GP:QDH50311.1;Name=QDH50311.1;Note=SSC: 16500 - 17453~Predicted by: G%26G~Length: 954~SD Score: 798%2C highest~CP: covers all CP~Longest ORF?: yes~gap/overlap: gap of 3~Blast: 1:1 match with KonjoTrouble and one other%2C 99%25 query %26 100%25 identity coverage~Function: upper collar protein~Function Source: blastp detects a conserved domain%2C evalue: 5.16e-88;gbkey=CDS;locus_tag=VIOLETTEMAD_34;product=upper collar protein;protein_id=QDH50311.1;transl_table=11
MN082624.1	Genbank	gene	17440	18507	.	+	.	ID=gene-VIOLETTEMAD_35;Name=VIOLETTEMAD_35;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_35
MN082624.1	Genbank	CDS	17440	18507	.	+	0	ID=cds-QDH50312.1;Parent=gene-VIOLETTEMAD_35;Dbxref=NCBI_GP:QDH50312.1;Name=QDH50312.1;Note=SSC: 17440 - 18507~Predicted by: G%26G~Length: 1068~SD Score: 525~CP: covers all CP~Longest ORF?: yes~gap/overlap: overlap of 13~Blast: 1:1 match with KonjoTrouble and 6 others%2C 99%25 query %26 100%25 identity coverage~Function: lower collar protein~Function Source: blastp detects a conserved domain%2C e-value: 3.65e-42;gbkey=CDS;locus_tag=VIOLETTEMAD_35;product=lower collar protein;protein_id=QDH50312.1;transl_table=11
MN082624.1	Genbank	gene	18522	20675	.	+	.	ID=gene-VIOLETTEMAD_36;Name=VIOLETTEMAD_36;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_36
MN082624.1	Genbank	CDS	18522	20675	.	+	0	ID=cds-QDH50313.1;Parent=gene-VIOLETTEMAD_36;Dbxref=NCBI_GP:QDH50313.1;Name=QDH50313.1;Note=SSC: 18522 - 20675~Predicted by: NI%2C original start was at 18600 but the blast matches weren't 1:1%2C changed start to 18522~Length: 2154~SD Score: 567~CP: covers almost all CP~gap/overlap: none~Longest ORF?: yes~Blast: 1:1 match with KonjoTrouble only%2C 99%25 query %26 100%25 identical coverage~Function: pre-neck appendage protein~Function Source: HHpred - multiple hits with evalue under 10^-05%2C top hit has 99.91 probablity %26 evalue 5.3e-24;gbkey=CDS;locus_tag=VIOLETTEMAD_36;product=pre-neck appendage protein;protein_id=QDH50313.1;transl_table=11
MN082624.1	Genbank	gene	20675	21913	.	+	.	ID=gene-VIOLETTEMAD_37;Name=VIOLETTEMAD_37;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_37
MN082624.1	Genbank	CDS	20675	21913	.	+	0	ID=cds-QDH50314.1;Parent=gene-VIOLETTEMAD_37;Dbxref=NCBI_GP:QDH50314.1;Name=QDH50314.1;Note=SSC: 20675 - 21913~Predicted by: G%26G~Length: 1239~SD Score: 525%2C not highest~CP: covers all CP~Longest ORF?: yes~gap/overlap: none~Blast: 1:1 match with KonjoTrouble and 4 others%2C 99%25 query %26 100%25 identity coverage~Function: lytic transglycosylace~Function Source: blastp detects conserved domain%2C e-value: 4.43e-60;gbkey=CDS;locus_tag=VIOLETTEMAD_37;product=morphogenesis protein;protein_id=QDH50314.1;transl_table=11
MN082624.1	Genbank	gene	21948	22097	.	+	.	ID=gene-VIOLETTEMAD_38;Name=VIOLETTEMAD_38;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_38
MN082624.1	Genbank	CDS	21948	22097	.	+	0	ID=cds-QDH50315.1;Parent=gene-VIOLETTEMAD_38;Dbxref=NCBI_GP:QDH50315.1;Name=QDH50315.1;Note=SSC: 21948 - 22097~Predicted by: G%26G~Length: 150~SD Score: 504~CP: covers all CP~gap/overlap: gap of 2~Longest ORF?: yes~Blast: 1:1 match with Claudi only%2C 98%25 query %26 100%25 identity coverage~Function: N/A~Function Source: N/A~Contains one predicted transmembrane helix%2C amino acids 7-29.~CDD 10^-3 hit to YvrJ family protein from Bacillus sp.~93%25 probability match to Holin BhlA in pfam.~The Phage_holin_BhlA family is a family of holin-like proteins from both bacteriophages and bacterial chromosomes. In bacteriophage%2C holins are small membrane proteins that accumulate and oligomerise to form non-specific lesions in the cytoplasmic membrane allowing the release of the second protein%2C endolysins%2C to access the peptidoglycan. Most holins share common structural features: two or three transmembrane domains separated by a beta-turn%2C a short hydrophilic N-terminus%2C a highly charged C-terminus and a dual translational start motif. The BhlA holin of Bacillus is found to be toxic to the host cell where the site of action of is on the cell membrane and causes bacterial death by cell membrane disruption;gbkey=CDS;locus_tag=VIOLETTEMAD_38;product=holin;protein_id=QDH50315.1;transl_table=11
MN082624.1	Genbank	gene	22099	22896	.	+	.	ID=gene-VIOLETTEMAD_39;Name=VIOLETTEMAD_39;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_39
MN082624.1	Genbank	CDS	22099	22896	.	+	0	ID=cds-QDH50316.1;Parent=gene-VIOLETTEMAD_39;Dbxref=NCBI_GP:QDH50316.1;Name=QDH50316.1;Note=SSC: 22099 - 22896~Predicted by: G%26G~Length: 798~SD Score: 714~CP: covers all CP~gap/overlap: gap of 2~Longest ORF?: yes~Blast: 1:1 match with KonjoTrouble %26 8 others - 99%25 query %26 100%25 identity coverage~Function: endolysin~Function Source: HHpred - multiple hits with e-value less than 10^-05%2C top hit has probability 100%25 %26 1.3e-37 evalue;gbkey=CDS;locus_tag=VIOLETTEMAD_39;product=endolysin;protein_id=QDH50316.1;transl_table=11
MN082624.1	Genbank	gene	23107	24207	.	+	.	ID=gene-VIOLETTEMAD_40;Name=VIOLETTEMAD_40;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_40
MN082624.1	Genbank	CDS	23107	24207	.	+	0	ID=cds-QDH50317.1;Parent=gene-VIOLETTEMAD_40;Dbxref=NCBI_GP:QDH50317.1;Name=QDH50317.1;Note=SSC: 23107 - 24207~Predicted by: G%26G~Length: 1101~SD Score: 567~CP: covers all CP~gap/overlap: gap of 80~Longest ORF?: no~Blast: 1:1 match with KonjoTrouble and 7 others%2C 99%25 query %26 100%25 identity coverage~Function: ATPase encapsidation protein~Function Source: blastp detects conserved domain%2C evalue - 9.4e-135;gbkey=CDS;locus_tag=VIOLETTEMAD_40;product=ATPase encapsidation protein;protein_id=QDH50317.1;transl_table=11
MN082624.1	Genbank	gene	24272	24418	.	-	.	ID=gene-VIOLETTEMAD_41;Name=VIOLETTEMAD_41;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_41
MN082624.1	Genbank	CDS	24272	24418	.	-	0	ID=cds-QDH50318.1;Parent=gene-VIOLETTEMAD_41;Dbxref=NCBI_GP:QDH50318.1;Name=QDH50318.1;Note=SSC: 24272 - 24418~Predicted by: G%26G~Length: 147~SD Score: 756~CP: covers all CP~gap/overlap: overlap of 3~Longest ORF?: yes~Blast: 1:1 match with KonjoTrouble and 1 other%2C 97%25 query %26 100%25 identity coverage~Function: N/A~Function Source: N/A;gbkey=CDS;locus_tag=VIOLETTEMAD_41;product=hypothetical protein;protein_id=QDH50318.1;transl_table=11
MN082624.1	Genbank	gene	24415	24600	.	-	.	ID=gene-VIOLETTEMAD_42;Name=VIOLETTEMAD_42;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_42
MN082624.1	Genbank	CDS	24415	24600	.	-	0	ID=cds-QDH50319.1;Parent=gene-VIOLETTEMAD_42;Dbxref=NCBI_GP:QDH50319.1;Name=QDH50319.1;Note=SSC: 24415 - 24600~Predicted by: G%26G~Length: 186~SD Score: 546~CP: covers almost all CP~gap/overlap: gap of 2~Longest ORF?: no~Blast: 1:1 match with QCM11 and one other%2C 98%25 query %26 100%25 identity coverage~Function: N/A~Function Source: N/A;gbkey=CDS;locus_tag=VIOLETTEMAD_42;product=hypothetical protein;protein_id=QDH50319.1;transl_table=11
MN082624.1	Genbank	gene	24602	24895	.	-	.	ID=gene-VIOLETTEMAD_43;Name=VIOLETTEMAD_43;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_43
MN082624.1	Genbank	CDS	24602	24895	.	-	0	ID=cds-QDH50320.1;Parent=gene-VIOLETTEMAD_43;Dbxref=NCBI_GP:QDH50320.1;Name=QDH50320.1;Note=SSC: 24602 - 24895~Predicted by: G%26G~Length: 294~SD Score: 609~CP: covers all CP~gap/overlap: none~Longest ORF?: no~Blast: 1:1 match with KonjoTrouble %26 6 others%2C 98%25 query %26 100%25 identity coverage~Function: N/A~Function Source: N/A;gbkey=CDS;locus_tag=VIOLETTEMAD_43;product=hypothetical protein;protein_id=QDH50320.1;transl_table=11
MN082624.1	Genbank	gene	24895	25038	.	-	.	ID=gene-VIOLETTEMAD_44;Name=VIOLETTEMAD_44;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_44
MN082624.1	Genbank	CDS	24895	25038	.	-	0	ID=cds-QDH50321.1;Parent=gene-VIOLETTEMAD_44;Dbxref=NCBI_GP:QDH50321.1;Name=QDH50321.1;Note=SSC: 24895 - 25038~Predicted by: G%26G~Length: 144~SD Score: 483~CP: covers all CP~gap/overlap: gap of 3~Longest ORF?: no~Blast: 1:1 match with KonjoTrouble %26 one other%2C 97%25 query %26 100%25 identity coverage~Function: N/A~Function Source: N/A;gbkey=CDS;locus_tag=VIOLETTEMAD_44;product=hypothetical protein;protein_id=QDH50321.1;transl_table=11
MN082624.1	Genbank	gene	25041	25328	.	-	.	ID=gene-VIOLETTEMAD_45;Name=VIOLETTEMAD_45;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_45
MN082624.1	Genbank	CDS	25041	25328	.	-	0	ID=cds-QDH50322.1;Parent=gene-VIOLETTEMAD_45;Dbxref=NCBI_GP:QDH50322.1;Name=QDH50322.1;Note=SSC: 25041 - 25328~Predicted by: G%26G~Length: 288~SD Score: 504~CP: covers all CP~gap/overlap: gap of 1~Longest ORF?:yes~Blast: 1:1 match with KonjoTrouble %26 6 others%2C 98%25 query %26 100%25 identity coverage~Function: N/A~Function Source: N/A;gbkey=CDS;locus_tag=VIOLETTEMAD_45;product=hypothetical protein;protein_id=QDH50322.1;transl_table=11
MN082624.1	Genbank	gene	25329	25763	.	-	.	ID=gene-VIOLETTEMAD_46;Name=VIOLETTEMAD_46;gbkey=Gene;gene_biotype=protein_coding;locus_tag=VIOLETTEMAD_46
MN082624.1	Genbank	CDS	25329	25763	.	-	0	ID=cds-QDH50323.1;Parent=gene-VIOLETTEMAD_46;Dbxref=NCBI_GP:QDH50323.1;Name=QDH50323.1;Note=SSC: 25329 - 25763~Predicted by: G%26G~Length: 435~SD Score: 504~CP: covers all CP~gap/overlap: N/A~Longest ORF?: yes~Blast: 1:1 match with KonjoTrouble %26 4 others%2C 99%25 query %26 100%25 identical coverage~Function: early protein~Function Source: HHpred%2C one significant hit%2C probability 100 %26 7.7e-35;gbkey=CDS;locus_tag=VIOLETTEMAD_46;product=early protein;protein_id=QDH50323.1;transl_table=11
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