locus-tag class description BT0005 ENOG4105C31 Tryptophanyl-tRNA synthetase BT0006 ENOG4105DSF transport protein BT0007 ENOG4106TCE BT0008 ENOG4105C8B TIGR00159 family BT0009 ENOG4105TKT ybbr family BT0010 ENOG41083V3 Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein (By similarity) BT0011 ENOG4108CER Uncharacterized protein conserved in bacteria (DUF2225) BT0012 ENOG4105DI7 Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs (By similarity) BT0013 ENOG4108UN4 phage SPO1 DNA polymerase-related protein BT0014 ENOG4105C25 Primosomal protein n' BT0015 ENOG4107S03 uridine kinase BT0017 ENOG410749K Uncharacterized protein conserved in bacteria (DUF2179) BT0021 ENOG4105E2N Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) (By similarity) BT0022 ENOG4105CKJ The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing (By similarity) BT0023 ENOG4105KA4 The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB (By similarity) BT0023A ENOG4105NHU Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group (By similarity) BT0025 ENOG4105CDY transcriptional regulatory protein BT0026 ENOG4105CN0 Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate (By similarity) BT0027 ENOG4105XBK BT0029 ENOG41082MQ UPF0178 protein BT0031 ENOG4105C3F Signal peptidase i BT0032 ENOG4105UK2 Sulfatase-modifying factor enzyme 1 BT0033 ENOG4108UH4 Binds specifically to the SsrA RNA (tmRNA) and is required for stable association of SsrA with ribosomes (By similarity) BT0035 ENOG4105DF5 DNA topoisomerase IV, subunit A BT0036 ENOG4105DNX Dna topoisomerase iv (Subunit b) BT0037 ENOG4105E9Q Acyl-transferase BT0039 ENOG4108W50 ppic-type peptidyL-prolyl cis-trans isomerase BT0040 ENOG4105BZU Histidine kinase BT0042 ENOG4108UTW Plays a role in the regulation of phosphate uptake BT0043 ENOG4106GEY BT0046 ENOG4108UH2 Endonuclease that specifically degrades the RNA of RNA- DNA hybrids (By similarity) BT0052 ENOG4105FE8 tRNA (Guanosine-2'-O-)-methyltransferase BT0053 ENOG4105D5S Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine (By similarity) BT0055 ENOG4105CP7 Triose-phosphate isomerase BT0056 ENOG4105BZA Phosphoglycerate kinase BT0057 ENOG4105C17 glyceraldehyde-3-phosphate dehydrogenase BT0059 ENOG4105C37 (CBS) domain BT0060 ENOG41082QW Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA (By similarity) BT0061 ENOG4105NGQ Thioredoxin BT0062 ENOG4108TSD Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit (By similarity) BT0063 ENOG4108120 Pasta domain protein BT0064 ENOG4105CAE Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP (By similarity) BT0065 ENOG4108Z02 Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions (By similarity) BT0068 ENOG4105DQF Hydrolase BT0069 ENOG4105C88 aminopeptidase BT0074 ENOG4105CG1 Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA (By similarity) BT0076 ENOG4105CCP Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC) BT0078 ENOG4105D60 lipoprotein releasing system transmembrane protein BT0080 ENOG4105CQU Part of the ABC transporter complex LolCDE involved in the translocation of mature outer membrane-directed lipoproteins, from the inner membrane to the periplasmic chaperone, LolA. Responsible for the formation of the LolA-lipoprotein complex in an ATP-dependent manner (By similarity) BT0084 ENOG4105C9B Cysteine desulfurase BT0086 ENOG4105C0P Mg chelatase subunit ChlI BT0087 ENOG4105C80 Catalyzes the reversible oxidation of malate to oxaloacetate (By similarity) BT0088 ENOG4105C4S Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner (By similarity) BT0089 ENOG4106B9X BT0092 ENOG4108WBK V-type ATP synthase subunit D BT0093 ENOG4108JIP Produces ATP from ADP in the presence of a proton gradient across the membrane. The V-type beta chain is a regulatory subunit (By similarity) BT0094 ENOG4107RVI Produces ATP from ADP in the presence of a proton gradient across the membrane. The V-type alpha chain is a catalytic subunit (By similarity) BT0095 ENOG41069SF BT0096 ENOG4108RY1 ATP synthase, subunit E BT0097 ENOG4105XMA BT0098 ENOG4105C98 muts2 protein BT0099 ENOG4105E06 May play a role in 30S ribosomal subunit biogenesis. Unusual circulary permuted GTPase that catalyzes rapid hydrolysis of GTP with a slow catalytic turnover (By similarity) BT0100 ENOG4105F03 Provides the (R)-glutamate required for cell wall biosynthesis (By similarity) BT0101 ENOG4105C5S asparaginyl-tRNA synthetase BT0103 ENOG4108UGV hypoxanthine phosphoribosyltransferase BT0104 ENOG4105C0H protease BT0105 ENOG4105CA1 Removes the N-terminal methionine from nascent proteins (By similarity) BT0107 ENOG4105M7K Involved in the transcription termination process (By similarity) BT0108 ENOG4107UUG Plays a major role in protein secretion by helping the post-translocational extracellular folding of several secreted proteins (By similarity) BT0109 ENOG4105CHU acetyl-coa acetyltransferase BT0111 ENOG4105CDU Replicative dna helicase BT0112 ENOG4105K9Q Binds to the 23S rRNA (By similarity) BT0113 ENOG4105VH8 Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit (By similarity) BT0114 ENOG4105Z8H single-stranded DNA-binding protein BT0115 ENOG41084AY Binds together with S18 to 16S ribosomal RNA (By similarity) BT0117 ENOG4108UT2 hemolysin iii BT0118 ENOG4105DZP Membrane-associated zinc metalloprotease BT0119 ENOG4105KNE Phosphatidate cytidylyltransferase BT0120 ENOG4105CR3 Catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic pyrophosphates generating different type of terpenoids (By similarity) BT0121 ENOG4108VCV Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another (By similarity) BT0122 ENOG4105CU7 Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome (By similarity) BT0123 ENOG4105CE9 30S ribosomal protein S2 BT0123A ENOG4108Z03 MAF-like protein BT0127 ENOG4105CAV thus facilitating recognition of the initiation point. It is needed to translate mRNA with a short Shine-Dalgarno (SD) purine-rich sequence (By similarity) BT0128 ENOG4105CAT Cytidine monophosphate kinase BT0129 ENOG4105FE9 pseudouridine synthase BT0131 ENOG4105C68 Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage (By similarity) BT0132 ENOG4107VNU Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides (By similarity) BT0135 ENOG4105CUT histidyl-tRNA synthetase BT0136 ENOG4105CJN penicillin-binding protein BT0137 ENOG4108IQE Amp-dependent synthetase and ligase BT0145 ENOG4107SQ8 Glycine betaine BT0146 ENOG4108IJ7 Glycine betaine BT0147 ENOG4105I03 Flagellin BT0151 ENOG4105CE4 GlcNAc 6-P deacetylase BT0152 ENOG4105CKA Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion (By similarity) BT0153 ENOG4105CK4 Destroys radicals which are normally produced within the cells and which are toxic to biological systems (By similarity) BT0154 ENOG4105CI6 Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving BT0160 ENOG4105CJ4 Catalyzes the interconversion of L-alanine and D- alanine. May also act on other amino acids (By similarity) BT0164 ENOG4105FEU K -dependent Na -Ca exchanger BT0166 ENOG4108IUJ 4-alpha-glucanotransferase BT0167 ENOG4105UYK outer membrane protein a BT0169 ENOG4105K9U however, it seems to stimulate more or less all the activities of the other two initiation factors, IF-2 and IF-3 (By similarity) BT0176 ENOG4105C7R Atpase associated with various cellular activities aaa_3 BT0177 ENOG41083HS Specifically methylates the N7 position of a guanine in 16S rRNA (By similarity) BT0178 ENOG4107RE5 NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 (By similarity) BT0179 ENOG4105C1H Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 (By similarity) BT0183 ENOG4105W5T Binds to the C-terminal region of flagellin, which is implicated in polymerization, and participates in the assembly of the flagellum (By similarity) BT0184 ENOG4105VM8 Could accelerate the degradation of some genes transcripts potentially through selective RNA binding (By similarity) BT0185 ENOG4105CPM Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine (By similarity) BT0186 ENOG4105K8N protein family UPF0079, ATPase BT0188 ENOG4108YZX Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit (By similarity) BT0189 ENOG41084MZ 50S ribosomal protein L35 BT0190 ENOG4108UUX IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins (By similarity) BT0194 ENOG4105F8V Hydrolase, tatD family BT0196 ENOG4105C8K Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA (By similarity) BT0197 ENOG4105EQY Methylates the class 1 translation termination release factors RF1 PrfA and RF2 PrfB on the glutamine residue of the universally conserved GGQ motif (By similarity) BT0198 ENOG4105CWR In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance (By similarity) BT0200 ENOG4105CPF Cell wall formation (By similarity) BT0201 ENOG4107EEN Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan (By similarity) BT0202 ENOG4108JU6 CBS domain protein BT0203 ENOG4107RIZ hflk protein BT0204 ENOG4105EPW hflc protein BT0206 ENOG4106H78 methyltransferase BT0211 ENOG4105DC6 This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. May act as a molecular matchmaker , a protein that promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of a final effector complex (By similarity) BT0214 ENOG4105DRH Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase (By similarity) BT0215 ENOG4107VUB phosphate binding protein BT0216 ENOG4105C8U phosphate abc transporter BT0217 ENOG4105E2Z phosphate ABC transporter (Permease BT0218 ENOG4105BZY Part of the ABC transporter complex PstSACB involved in phosphate import. Responsible for energy coupling to the transport system (By similarity) BT0220 ENOG4105CIM Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain (By similarity) BT0222 ENOG4108Q79 6-phosphogluconolactonase (EC 3.1.1.31) BT0225 ENOG4105CEH Catalyzes the synthesis of dihydrouridine a modified base found in the D-loop of most tRNAs (By similarity) BT0226 ENOG4105CGR Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) (By similarity) BT0228 ENOG4107QYI peptidase BT0230 ENOG4105C4P Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template (By similarity) BT0232 ENOG41083IJ DNA-binding protein BT0233 ENOG41086BH Binds directly to 16S ribosomal RNA (By similarity) BT0235 ENOG4105C3G gtp-binding protein BT0237 ENOG4107RI4 Transfers the fatty acyl group on membrane lipoproteins (By similarity) BT0239 ENOG4105ZMH deoxynucleoside kinase BT0240 ENOG4105D8C Channel that permits osmotically driven movement of water in both directions. It is involved in the osmoregulation and in the maintenance of cell turgor during volume expansion in rapidly growing cells. It mediates rapid entry or exit of water in response to abrupt changes in osmolarity (By similarity) BT0241 ENOG4108JQ0 Key enzyme in the regulation of glycerol uptake and metabolism (By similarity) BT0241A ENOG4105CXY transporter BT0241B ENOG4106MZZ glycerophosphoryl diester phosphodiesterase BT0243 ENOG4105C6V Glycerol-3-phosphate dehydrogenase BT0246 ENOG4105DR5 peptidase BT0247 ENOG4108V82 Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions (By similarity) BT0248 ENOG4105DPN Oligoendopeptidase f BT0249 ENOG4105VE1 Phosphatidylcholine synthase BT0250 ENOG41083CW dedA protein BT0251 ENOG4105C8T Leucyl-tRNA synthetase BT0253 ENOG4105C6P ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner (By similarity) BT0254 ENOG4105C2R Single-stranded-DNA-specific exonuclease (RecJ) BT0255 ENOG4105DR5 peptidase BT0256 ENOG41084YP 30S ribosomal protein S21 BT0258 ENOG4105DWR Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin (By similarity) BT0262 ENOG4105E4C Lytic transglycosylase catalytic BT0263 ENOG4105C3F Signal peptidase i BT0264 ENOG4105CFG Acts as a chaperone (By similarity) BT0265 ENOG4106FP0 BT0269 ENOG4107R21 site-determining protein BT0270 ENOG4105FZW flagellar biosynthesis regulator FlhF BT0271 ENOG4105D7B Flagellar biosynthesis protein flha BT0272 ENOG4105D00 flagellar biosynthetic protein flhB BT0273 ENOG4105D19 Role in flagellar biosynthesis (By similarity) BT0274 ENOG4105VS8 Flagellar biosynthetic protein fliq BT0275 ENOG4105CN7 Flagellar biosynthetic protein FliP BT0277 ENOG4105DD5 Flagellar motor switch protein BT0278 ENOG4105D24 FliM is one of three proteins (FliG, FliN, FliM) that forms the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the motor that determine the direction of flagellar rotation (By similarity) BT0279 ENOG41064SM flagellar basal body-associated protein flil BT0280 ENOG4107XSU flagellar motor protein motb BT0281 ENOG4107R0U MotA TolQ exbB proton channel BT0283 ENOG4105BZE flagellar hook protein, FlgE BT0284 ENOG410822I Flagellar hook capping protein BT0288 ENOG4108JIR Flagellum-specific ATP synthase BT0289 ENOG4105W2B Flagellar assembly protein BT0290 ENOG4105CS3 FliG is one of three proteins (FliG, FliN, FliM) that forms the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the motor that determine the direction of flagellar rotation (By similarity) BT0291 ENOG4107U2N Flagellar M-ring protein BT0292 ENOG41069WH flagellar hook-basal body complex BT0293 ENOG4108ZC9 flagellar basal-body rod protein flgc BT0294 ENOG4105Q75 Structural component of flagellum, the bacterial motility apparatus. Part of the rod structure of flagellar basal body (By similarity) BT0295 ENOG4105C4N this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N-terminal part of its protein substrates and unfolds these before they are guided to HslV for hydrolysis (By similarity) BT0296 ENOG4108R5P Protease subunit of a proteasome-like degradation complex believed to be a general protein degrading machinery (By similarity) BT0297 ENOG4105C79 DNA protecting protein DprA BT0298 ENOG4106095 tetratricopeptide BT0299 ENOG4105CDK Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity (By similarity) BT0300 ENOG4105CIT This protein may be involved in anomalous filament growth. May be a component of the septum (By similarity) BT0302 ENOG4105CNI cell division protein FtsW BT0303 ENOG4105CPY First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan (By similarity) BT0304 ENOG4107EES Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide the precursor of murein (By similarity) BT0306 ENOG4105CGJ Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA (By similarity) BT0309A ENOG4105C4F May be involved in recombinational repair of damaged DNA (By similarity) BT0311 ENOG4105F91 Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus (By similarity) BT0312 ENOG4108V3W Chemotaxis protein, CheW BT0313 ENOG4105F7M Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit (By similarity) BT0328 ENOG4107QSI ABC, transporter BT0329 ENOG4108J59 Extracellular solute-binding protein, family 5 BT0332 ENOG4105CJM Binding-protein-dependent transport systems, inner membrane component BT0333 ENOG4105C2T Binding-protein-dependent transport systems, inner membrane component BT0335 ENOG4108JQ7 (ABC) transporter BT0337 ENOG4105C70 Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis (By similarity) BT0338 ENOG4108UJD 30S ribosomal protein S9 BT0339 ENOG4108UM5 This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly (By similarity) BT0340 ENOG4105YUD BT0341 ENOG4105CHT Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) (By similarity) BT0342 ENOG4105C3P Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) (By similarity) BT0344 ENOG4105C4R Atp-dependent dna helicase BT0346 ENOG4107Y7W Participates in the translocation of lipoproteins from the inner membrane to the outer membrane. Only forms a complex with a lipoprotein if the residue after the N-terminal Cys is not an aspartate (The Asp acts as a targeting signal to indicate that the lipoprotein should stay in the inner membrane) (By similarity) BT0348 ENOG4105CA9 Pyruvate kinase BT0350 ENOG410851C 50S ribosomal protein l28 BT0358 ENOG410852B Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit (By similarity) BT0359 ENOG4105CN1 protease BT0362 ENOG4107WGG Transfers the N-acyl diglyceride group on what will become the N-terminal cysteine of membrane lipoproteins (By similarity) BT0363 ENOG4105BZU Histidine kinase BT0366 ENOG4105DFM M18 family aminopeptidase BT0368 ENOG4105CSF NADPH-dependent glycerol-3-phosphate dehydrogenase BT0369 ENOG4105C2Z ATP-dependent CLP protease ATP-binding subunit BT0370 ENOG4105DA0 Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) (By similarity) BT0371 ENOG4105D9H Catalyzes the attachment of glycine to tRNA(Gly) (By similarity) BT0372 ENOG4105C20 Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) (By similarity) BT0374 ENOG4105CPI Metal Dependent Phosphohydrolase BT0375 ENOG4105DUF Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S- adenosylhomocysteine (SAH AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively (By similarity) BT0376 ENOG4105CPH Catalyzes the formation of S-adenosylmethionine from methionine and ATP BT0377 ENOG4106762 Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5- dihydroxy-2,3-pentadione (DPD) (By similarity) BT0379 ENOG4105K59 histidine triad (HIT) protein BT0380 ENOG4105CJW magnesium transporter BT0381 ENOG4107MD5 glycoside hydrolase, family BT0382 ENOG4107TFB basic membrane BT0384 ENOG4107TFB basic membrane BT0386 ENOG4108UHY One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, probably blocks exit of the E-site tRNA (By similarity) BT0387 ENOG4108UKE Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit (By similarity) BT0388 ENOG4105D27 DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (By similarity) BT0389 ENOG4108IIJ DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (By similarity) BT0390 ENOG4105KBC Seems to be the binding site for several of the factors involved in protein synthesis and appears to be essential for accurate translation (By similarity) BT0391 ENOG4108VZM 50s ribosomal protein L10 BT0392 ENOG4105C64 Binds directly to 23S rRNA. The L1 stalk is quite mobile in the ribosome, and is involved in E site tRNA release (By similarity) BT0393 ENOG4108UIK This protein binds directly to 23S ribosomal RNA (By similarity) BT0394 ENOG4105E5V Participates in transcription elongation, termination and antitermination (By similarity) BT0396 ENOG41084ED 50S ribosomal protein L33 BT0402 ENOG4107R1M Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) (By similarity) BT0407 ENOG4105EIP mannose-6-phosphate isomerase BT0408 ENOG4105DM4 PTS System BT0413A ENOG4107XH4 PPIases accelerate the folding of proteins (By similarity) BT0414 ENOG4105BZU Histidine kinase BT0415 ENOG4105CMP catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR (By similarity) BT0417 ENOG4105CC8 Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism (By similarity) BT0419 ENOG4105DW3 Response regulator receiver modulated metal dependent phosphohydrolase BT0420 ENOG4105BZU Histidine kinase BT0421A ENOG4108UGV hypoxanthine phosphoribosyltransferase BT0421B ENOG4105C91 Plays an important role in the de novo pathway of purine nucleotide biosynthesis BT0421C ENOG4107RAW 3-carboxy-cis-cis-muconate cycloisomerase BT0422 ENOG4108VX5 3-methyladenine DNA glycosylase BT0422A ENOG4105DNJ May play a role in the repair of endogenous alkylation damage (By similarity) BT0427 ENOG4105CU0 Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA (By similarity) BT0431 ENOG4107QJE Chromosome Partitioning Protein BT0434 ENOG4105FA0 parb-like partition protein BT0435 ENOG4105C24 DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings (By similarity) BT0436 ENOG4105C7D DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings (By similarity) BT0437 ENOG4105CI4 it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box) 5'-TTATC CA A CA A-3'. DnaA binds to ATP and to acidic phospholipids (By similarity) BT0438 ENOG4105CZ8 DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA, it slides freely (bidirectional and ATP- independent) along duplex DNA (By similarity) BT0438A ENOG4105C3X it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP (By similarity) BT0440 ENOG4105VG0 50S ribosomal protein l34 BT0441 ENOG410604M RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme (By similarity) BT0442 ENOG4105DHW Required for the insertion and or proper folding and or complex formation of integral membrane proteins into the membrane. Involved in integration of membrane proteins that insert both dependently and independently of the Sec translocase complex, as well as at least some lipoproteins BT0445 ENOG4105D2N Aldolase BT0446 ENOG4105C9M aspartyl-trna synthetase BT0447 ENOG4107RWR Sodium hydrogen exchanger BT0451 ENOG4105CAH Chromate BT0453 ENOG4105D64 Mechanosensitive ion channel BT0454 ENOG4107V8I Glycosyl transferases group 1 BT0457 ENOG4105CII The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision (By similarity) BT0461 ENOG4107QMP dna polymerase iii subunits gamma and tau BT0462 ENOG4107Q1J Binds to DNA and alters its conformation. May be involved in regulation of gene expression, nucleoid organization and DNA protection (By similarity) BT0462A ENOG4105F3K May play a role in DNA repair. It seems to be involved in an RecBC-independent recombinational process of DNA repair. It may act with RecF and RecO (By similarity) BT0463 ENOG4108UGX Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate (By similarity) BT0466 ENOG4108IUZ ABC transporter BT0467 ENOG4107RSD Multi-copper polyphenol oxidoreductase laccase BT0468 ENOG4107XF7 NGG1p interacting factor 3 protein, NIF3 BT0469 ENOG410835P This protein specifically catalyzes the removal of signal peptides from prolipoproteins (By similarity) BT0472 ENOG4105CDF Cell wall formation. Adds enolpyruvyl to UDP-N- acetylglucosamine (By similarity) BT0473 ENOG4107QJQ Mate efflux family protein BT0476 ENOG4105CGV This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis (By similarity) BT0477 ENOG4108Z10 Involved in the binding of tRNA to the ribosomes (By similarity) BT0478 ENOG4105EEE One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit (By similarity) BT0479 ENOG4106U5A One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome (By similarity) BT0480 ENOG41080UE One of the early assembly proteins it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome (By similarity) BT0481 ENOG4105CFD One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity BT0482 ENOG4105K7S Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA (By similarity) BT0483 ENOG41082G4 The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome (By similarity) BT0484 ENOG4105CKE Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation (By similarity) BT0485 ENOG4108R70 Binds 23S rRNA and is also seen to make contacts with the A and possibly P site tRNAs (By similarity) BT0486 ENOG41086S0 50s ribosomal protein l29 BT0487 ENOG4105K87 One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal BT0488 ENOG4108UNN Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome (By similarity) BT0489 ENOG4105KAR One of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit (By similarity) BT0490 ENOG4105CW6 This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits BT0491 ENOG41082QR Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site (By similarity) BT0492 ENOG4108UJY One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit (By similarity) BT0493 ENOG4108R5J This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7 L12 stalk, and near the tRNA binding site of the peptidyltransferase center (By similarity) BT0494 ENOG4105K4C This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance (By similarity) BT0495 ENOG4108RA9 Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body (By similarity) BT0497 ENOG4108UZ0 Binds to the 23S rRNA (By similarity) BT0498 ENOG4105CGG The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently (By similarity) BT0500 ENOG4108Z04 Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits BT0501 ENOG4108UHH Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome (By similarity) BT0502 ENOG4105CTF DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (By similarity) BT0503 ENOG4108ZT0 50S ribosomal protein l17 BT0504 ENOG4105E4Y Endoribonuclease that initiates mRNA decay (By similarity) BT0505 ENOG4105D48 metallophosphoesterase BT0506 ENOG4105DX0 Hemolysin A BT0508 ENOG4105DKZ GTPase that plays an essential role in the late steps of ribosome biogenesis (By similarity) BT0514 ENOG4107TIA phenylalanyl-tRNA synthetase (beta subunit) BT0517 ENOG4105BZ5 ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins (By similarity) BT0519 ENOG4105K90 Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ BT0522 ENOG4105C4K Nad synthetase BT0522A ENOG4108MJI response regulator BT0527 ENOG4107QNB Catalyzes the phosphorylation of pantothenate (Pan), the first step in CoA biosynthesis (By similarity) BT0533 ENOG4108H5C Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate (By similarity) BT0534 ENOG4105CGK Exodeoxyribonuclease III BT0536 ENOG4107RAN Peptidase, M16 BT0539 ENOG4105HFZ Membrane BT0540 ENOG4105CEJ Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (By similarity) BT0544 ENOG4105C5T Phosphoribosyl pyrophosphate synthase BT0547 ENOG41083NY Catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A (By similarity) BT0548 ENOG4105C2M dna polymerase I BT0551 ENOG4107Z9R response regulator BT0552 ENOG4105C77 DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA (By similarity) BT0557 ENOG4105KZ7 phosphocarrier protein hpr BT0558 ENOG4105BZ3 General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr) (By similarity) BT0559 ENOG4105C5Y PTS System BT0560 ENOG4105CJX Molecular chaperone. Has ATPase activity (By similarity) BT0561 ENOG4105C7Q Catalyzes the oxidative decarboxylation of 6- phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH (By similarity) BT0565 ENOG4108V3W Chemotaxis protein, CheW BT0567 ENOG4105CBS Chea signal transduction histidine kinase BT0568 ENOG4105CMP catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR (By similarity) BT0570 ENOG4105BZU Histidine kinase BT0570A ENOG4107QSE Dipeptidase BT0571 ENOG4105C41 Catalyzes the reversible phosphorylation of UMP to UDP (By similarity) BT0572 ENOG4105K64 Glycosyl transferase, family 2 BT0573 ENOG4108IUW ABC transporter BT0575 ENOG4105C8D Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen (By similarity) BT0578 ENOG4105C8Q Methyl-accepting chemotaxis BT0579 ENOG4105C0B DNA polymerase III (alpha subunit) BT0581 ENOG4105CB5 ATP-dependent DNA helicase recG BT0583 ENOG4105C3S Mate efflux family protein BT0585 ENOG4105DMZ Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) (By similarity) BT0586 ENOG4108TV1 Catalyzes the incorporation of amino acid(s) into the interchain peptide bridge of peptidoglycan, using aminoacyl-tRNA as amino acid donor (By similarity) BT0587 ENOG4105CKH Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation (By similarity) BT0590 ENOG4105D1X Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits (By similarity) BT0593 ENOG4108IQE Amp-dependent synthetase and ligase BT0594 ENOG4105C75 arginyL-tRNA synthetase BT0596 ENOG4105C8Q Methyl-accepting chemotaxis BT0597 ENOG4105EC8 Methyl-accepting chemotaxis BT0598 ENOG4107QWW Cell wall formation (By similarity) BT0599 ENOG4105C8N cysteinyl-tRNA synthetase BT0601 ENOG4105C65 Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF- independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism (By similarity) BT0602 ENOG4105BZ5 ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins (By similarity) BT0605 ENOG4105DZ1 carboxypeptidase BT0606 ENOG4108Z4W Probably deamidates glutamine residues to glutamate on methyl-accepting chemotaxis receptors (MCPs), playing an important role in chemotaxis (By similarity) BT0608 ENOG4105CIN aminoacyl-histidine dipeptidase BT0610 ENOG4105DEA Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation BT0611 ENOG4105CCQ Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins (By similarity) BT0612 ENOG4105CHN ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of ClpP (By similarity) BT0613 ENOG4105C6P ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner (By similarity) BT0615 ENOG4105G6W One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit (By similarity) BT0621 ENOG4108Z0Z intracellular protease Pfpi family BT0622 ENOG4105C6H Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction (By similarity) BT0623 ENOG4108JA2 transcriptioN-repair coupling factor BT0629 ENOG4105DM4 PTS System BT0630 ENOG4105DE4 1-phosphofructokinase BT0632 ENOG4107QYX exodeoxyribonuclease v alpha BT0633 ENOG4107X59 exodeoxyribonuclease V beta BT0634 ENOG4105DSY exodeoxyribonuclease V, gamma BT0635 ENOG4105DAP Nicotinate phosphoribosyltransferase BT0636 ENOG4105D8W glucose-6-phosphate 1-dehydrogenase BT0638 ENOG4105DFD Na H antiporter BT0640 ENOG4105D38 putrescine abc transporter BT0641 ENOG4105F38 ABC transporter, permease BT0642 ENOG4105C53 Part of the ABC transporter complex PotABCD involved in spermidine putrescine import. Responsible for energy coupling to the transport system (By similarity) BT0643 ENOG4105C9W Required for a late step of 50S ribosomal subunit assembly. Has GTPase activity (By similarity) BT0644 ENOG4105C9S Converts N-acetylmannosamine-6-phosphate (ManNAc-6-P) to N-acetylglucosamine-6-phosphate (GlcNAc-6-P) (By similarity) BT0649 ENOG4105CJ9 Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions (By similarity) BT0651 ENOG4105VIE preprotein translocase, subunit YajC BT0652 ENOG4107QN8 Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA (By similarity) BT0653 ENOG4107XHD Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA (By similarity) BT0655 ENOG4105BZ5 ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins (By similarity) BT0656 ENOG4105CSA Coproporphyrinogen iii oxidase BT0657 ENOG4105E66 phosphoriboisomerase A BT0658 ENOG4105DKJ Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate (By similarity) BT0659 ENOG4105DK7 lysyl-tRNA synthetase BT0660 ENOG4105CWT An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism (By similarity) BT0668 ENOG4107NGC Flagellar filament outer layer protein BT0669 ENOG4105CBS Chea signal transduction histidine kinase BT0670 ENOG4108V3W Chemotaxis protein, CheW BT0672 ENOG4107Z9R response regulator BT0676 ENOG4108VXP Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stress (By similarity) BT0677 ENOG4108JQ9 ABC transporter BT0678 ENOG4105UIB (ABC) transporter BT0680 ENOG4105C8Q Methyl-accepting chemotaxis BT0681 ENOG4105C8Q Methyl-accepting chemotaxis BT0682 ENOG4105CCJ Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34 (By similarity) BT0683 ENOG4105F99 Hydroxymethylglutaryl-CoA synthase BT0684 ENOG4106UJV Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its allylic isomer, dimethylallyl diphosphate (DMAPP) (By similarity) BT0685 ENOG4107EEU hydroxymethylglutaryL-CoA reductase BT0691 ENOG4105CEJ Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (By similarity) BT0695 ENOG4105K5M 30s ribosomal protein S16 BT0696 ENOG4105VB6 UPF0109 protein BT0697 ENOG4107GQ3 An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes (By similarity) BT0698 ENOG4105D6X Specifically methylates guanosine-37 in various tRNAs (By similarity) BT0699 ENOG4108YY1 This protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site (By similarity) BT0702 ENOG4108ZEF Reversibly transfers an adenylyl group from ATP to 4'- phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate (By similarity) BT0704 ENOG41084E5 Carrier of the growing fatty acid chain in fatty acid biosynthesis (By similarity) BT0705 ENOG4108ZBM Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon (By similarity) BT0706 ENOG4105D4J Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate BT0709 ENOG4105HHA aminodeoxychorismate lyase BT0712 ENOG4107QVY Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released (By similarity) BT0715 ENOG4105DFF Rod shape-determining protein mreb BT0718 ENOG4105CJN penicillin-binding protein BT0719 ENOG4105CNI cell division protein FtsW BT0720 ENOG4105C22 threonyL-tRNA synthetase BT0721 ENOG4105BZQ cdp-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase BT0726 ENOG4105D1F ATP-binding protein BT0727 ENOG4105CTQ phosphohexokinase BT0728 ENOG4107QMW pyridine nucleotide-disulfide oxidoreductase BT0730 ENOG4107QP8 phosphohexose isomerase BT0731 ENOG4105FA0 parb-like partition protein BT0732 ENOG4108JQC penicillin-binding protein 1A BT0734 ENOG4105EK0 Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine (By similarity) BT0735 ENOG4105K6T rare lipoprotein A BT0735A ENOG4105DVT helicase BT0737 ENOG4105CEH Catalyzes the synthesis of dihydrouridine a modified base found in the D-loop of most tRNAs (By similarity) BT0738 ENOG4105CA4 amino acids such as threonine, to avoid such errors, it has a posttransfer editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner (By similarity) BT0740 ENOG4105CWG Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl group from S-adenosyl-L-methionine to nm(5)s(2)U34, to form mnm(5)s(2)U34 (By similarity) BT0741 ENOG4105K5Y Binds to Cpn60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter (By similarity) BT0742 ENOG4105C5H ABC transporter BT0747 ENOG4105CJM Binding-protein-dependent transport systems, inner membrane component BT0754 ENOG4105CJ1 ABC, transporter BT0755 ENOG4105IES Zinc phosphodiesterase, which displays some tRNA 3'- processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA (By similarity) BT0756 ENOG41080HI glycine oxidase BT0757 ENOG4108S10 Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins (By similarity) BT0761 ENOG4105DR5 peptidase BT0763 ENOG4105C1W two component, sigma54 specific, transcriptional regulator, Fis family BT0764 ENOG4105BZU Histidine kinase BT0765 ENOG4107QRG dna polymerase iii BT0767 ENOG4105DVQ Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc- (pentapeptide)GlcNAc (lipid intermediate II) (By similarity) BT0768 ENOG4107XGF functions in a salvage pathway. Uses pyridoxamine (By similarity) BT0769 ENOG4105CPM Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine (By similarity) BT0771 ENOG4105DG1 Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released (By similarity) BT0772 ENOG4105D92 Assembles around the rod to form the L-ring and probably protects the motor basal body from shearing forces during rotation (By similarity) BT0774 ENOG4105CGA flagellar basal-body rod protein FlgG BT0775 ENOG4105CGA flagellar basal-body rod protein FlgG BT0778 ENOG4105KK9 This protein binds to 23S rRNA in the presence of protein L20 (By similarity) BT0780 ENOG4105K46 50S ribosomal protein l27 BT0781 ENOG4105C9R An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate (By similarity). It may play a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control BT0782 ENOG4107X4V Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD) (By similarity) BT0785 ENOG4105K4W Could be involved in septation (By similarity) BT0786 ENOG4107TVT This is one of the proteins that binds to the 5S RNA in the ribosome where it forms part of the central protuberance (By similarity) BT0787 ENOG4108ZPD The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis (By similarity) BT0788 ENOG4107THF Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine (By similarity) BT0790A ENOG4105C0H protease BT0793 ENOG410805Y Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis (By similarity) BT0795 ENOG4105E1Z Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane BT0797 ENOG4105D86 that it carries out the mismatch recognition step. This protein has a weak ATPase activity (By similarity) BT0798 ENOG4105MJ2 Competence protein BT0799 ENOG410854D Required for maturation of 30S ribosomal subunits (By similarity) BT0800 ENOG4105CHV Transcription elongation factor NusA BT0801 ENOG4107QHU One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex (By similarity) BT0802 ENOG41080JB Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Essential for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA (By similarity) BT0803 ENOG4105D0T Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs (By similarity) BT0804 ENOG4105K77 Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome (By similarity) BT0805 ENOG4105C62 Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction (By similarity) BT0809 ENOG4105C6U Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (By similarity) BT0810 ENOG4105CJR Integral membrane protein (MviN BT0812 ENOG4105CJS Phosphopantothenoylcysteine decarboxylase BT0814 ENOG4108IA2 Sodium pantothenate symporter BT0817 ENOG4105DFU Cell wall formation (By similarity) BT0819 ENOG4108Z4J Cytidine monophosphate kinase BT0821 ENOG4105DKX Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) (By similarity) BT0826A ENOG4105CI1 PTS System BT0827 ENOG4105CQ7 ATP-dependent helicase BT0828 ENOG4105C73 Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity) BT0829 ENOG4107RFY SbcCD D subunit BT0831 ENOG41066F8 ROK family BT0831A ENOG4106N3A copper homeostasis protein cutc BT0833 ENOG4105C07 amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) (By similarity) BT0834 ENOG4105C2Z ATP-dependent CLP protease ATP-binding subunit BT0837 ENOG4105C5U The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate (By similarity) BT0841 ENOG4105CEU Arginine dihydrolase BT0842 ENOG4105DBV ornithine carbamoyltransferase BT0843 ENOG4105CSX c4-dicarboxylate anaerobic carrier BT0843A ENOG4105C5B carbamate kinase BT0_B06 ENOG4105DUF Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S- adenosylhomocysteine (SAH AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively (By similarity) BT0_B09 ENOG4105C7N Xanthine uracil vitamin C permease BT0_B21 ENOG4105C2U cobyrinic Acid a,c-diamide synthase BT0_D05 ENOG4107QJE Chromosome Partitioning Protein BT0_E02 ENOG4105C2U cobyrinic Acid a,c-diamide synthase BT0_F06 ENOG4107QJE Chromosome Partitioning Protein BT0_P25 ENOG4107QJE Chromosome Partitioning Protein BT0_P29 ENOG4105Z8H single-stranded DNA-binding protein BT0_U15 ENOG4105C2U cobyrinic Acid a,c-diamide synthase