LOCUS HM640230 16748 bp DNA linear PHG 17-NOV-2014 DEFINITION Clostridium phage CpV1 hypothetical proteins, DNA polymerase, hypothetical protein, putative transcriptional regulator, hypothetical proteins, DNA encapsidation protein, collar protein, connector protein, hypothetical protein, endolysin, tail protein, lysozyme-peptidase, hypothetical protein, head protein, hypothetical proteins, and putative DNA binding protein genes, complete cds. ACCESSION HM640230 VERSION HM640230.1 KEYWORDS . SOURCE Clostridium phage CpV1 ORGANISM Clostridium phage CpV1 Viruses; dsDNA viruses, no RNA stage; Caudovirales; Podoviridae. REFERENCE 1 (bases 1 to 16748) AUTHORS Volozhantsev,N.V., Verevkin,V.V., Bannov,V.A., Krasilnikova,V.M., Myakinina,V.P., Zhilenkov,E.L., Svetoch,E.A., Stern,N.J., Oakley,B.B. and Seal,B.S. TITLE The genome sequence and proteome of bacteriophage PhiCPV1 virulent for Clostridium perfringens JOURNAL Virus Res. 155 (2), 433-439 (2011) PUBMED 21144870 REFERENCE 2 (bases 1 to 16748) AUTHORS Volozhantsev,N.V., Bannov,V.A., Verevkin,V.V., Krasilnikova,V.M. and Seal,B.S. TITLE Direct Submission JOURNAL Submitted (07-JUL-2010) Molecular Microbiology, State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region 142279, Russia FEATURES Location/Qualifiers source 1..16748 /organism="Clostridium phage CpV1" /mol_type="genomic DNA" /strain="CpV1" /host="Clostridium perfringens" /db_xref="taxon:926066" repeat_region 1..25 /note="ITR" /rpt_type=inverted /rpt_type=terminal CDS 251..607 /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ADR30474.1" /translation="MKRVFETAHALTRQFISTYGGDYRGQFIVTLKMLCEEKKNIKLR LERLIEEKKTKGITSINVYFKNGVSLFGDVDCIDVNNNNFIFRDFLKEKYIKTTRRTS FHSLMKIDYRLVDWVS" CDS 636..794 /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ADR30475.1" /translation="MRKIFEGILLCVAFILSAISFIYLYNNTFKSTLIFILLVLAIII LLSNILYD" CDS 787..1029 /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ADR30476.1" /translation="MIRGDKLKLKEFIENFCYKDTDIKIRGISNKSNSITYMIFQGYD YDIASETEVFSLIEGLNVIDVKVEREYLVIMVDDMI" CDS 1103..1504 /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ADR30477.1" /translation="MKYKVTKLNVINQEHYDIIKDKCFTELSEIPLTTHYVLDTETCR TRKEMLLDTNMEQEVKTYAVGVMSVGDKDNNMFVCETWEMFFENIYKTVQANLKFYLK NNQKRDKKNLDVTIKVGVHNVKYENSFMHMF" CDS 1579..3354 /note="similar to DNA polymerase of Bacillus phi29-family phages" /codon_start=1 /transl_table=11 /product="DNA polymerase" /protein_id="ADR30478.1" /translation="MLDTGCYNITQANGVHYGAKVCLKDTIINQKIISKGKNKGKLVE QEIKIYLEFWDTLKVAQTSLEKAHKFVEMDEMYYKLNKEYDYDKVRANGHILDALELE YLYNDLYATKKLVEDFYYDTLLTFKGKEKTIYLNDNLKTSASIAFNTAIAYTFSNGES EITDIKGARELFNEHFEIEKRIELKQELKDMERLSYRGGWTHANLKHINNIVEKNGTS IDKNSSYPSQMCFGLLPYGAPMEFNSIEDMREYMKENELTMDDHVFIINTGFDYVRPK CKKYDLEVIQLGSVNATMEFKKMSFEHEGKVVQNGNTFINRNYWNEDNKIQIYKKGCE EIANYNFTITNVEYDFLNKYYEFGYHGEIDFIDDEITFEGLRTGKVLLYKAEKGLLRE YIEELYLMKDEVKRRINNGEKGLDALYSSIKVLLNSLYGKFGSKVRKTLDNLFLNDEG IFSWDIGCKKELRQNENEIELLEYDSEEYYVPFASFVTAYARVEIQTMVIEAIGVENF LYTDTDSIYCSLTKEQLIEALKKINYELDGYELGKWDLEHVFNKFKSLGAKKYMLYDT RTKNICVACAGVPKKAQSILAKPRI" CDS 3548..4189 /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ADR30479.1" /translation="MAKRTTKQQFMKKWAKAVKNNSYIFLQDKDNMKKAQKYFGLSDE YVTIVGNKAKANYQIENKKKIVNERKTKLRDSLLKDGTVDTTDVKKIINNDRLYKKLN TRNVRSSVNKAIQTEFFNIISNIGLFKDNGAFRRALKGNDTPIKEFKKKVLEAIQKSD KPFELLLSFSERIDKAFELIYEAYPEGALNSVQGHRVVNKFIDIIETEMLRSV" CDS 4192..4425 /codon_start=1 /transl_table=11 /product="putative transcriptional regulator" /protein_id="ADR30480.1" /translation="MKIIKTTRNGKTKHVVLEGEHITKEQLKELLKTTNGVRFSYFGS EYRNFQEIKDMLEITIGLKENYILITKRRGAKF" CDS 4425..4586 /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ADR30481.1" /translation="MNEYCKFKFKGEIYYGTITEEHVDYYIIKVNNKFPHYFFKDDVT IIYKMTYDR" CDS 4600..5106 /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ADR30482.1" /translation="MKGLIYTLLFMFSIGGGVLMALPTNEIEPQPIKETITVEKELKE MENELTLTKEFLLKSYESSCEAFNKLHLNDINKFDDQVALEYLEEFDYWLNELNELTE LLKKNLPYEEFKIMEFRKLSLMKDKEAQEEDIIHQYEGTQLLNLMLNRFRSEYVYEIC KILVNEYM" CDS complement(5133..7136) /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ADR30483.1" /translation="MINKLNKWDLNCSVFNSYDFDDCMSLNELLCRFFTKINECIEAS NKSLTFLEWLYEVGLKQEVVTLLTQWKDNGTLAELISEQILTEIKQNIENNKNAIEQL QLKDTTHDEKIQALETKDNEILGDIETLKSKDTELEGSISSVSSDIETINVKIEEAKN ELKILSEQILNIVNEMKIKNGVDITFFGAKDDVNYDSTQSIINAINFAKTNKYNTLFI PNGKYYVTQSLNTKGLYVVGVGTPEIPFMTWDYTRPSSDLNDFKKYFSLCQGSIIGSD YDGNIFSNGLNATNIGIIGNRRATNQNGVSQTNGGDLINLSNCRIHGCGKDGVNALYG LIATIVDNRTWLYQNGRHGIYIGKESGGYTGETNFITIKDSFINRNEKDGIKINSLGR SIHIENVDLEQNGEPSDTQRHKGNDVYSIVYGCRINIDNDGTNFTGGSIVFNNNYSEE TFGLLYLETPTNRITNGVTINNNYWRPLNQNSYSCGVALKGWIENIEIMNNNLYGKDE VKILDTNVYAIKTSNNVSGGQSNPCVWKQKADYYGSIVEIGATGKSELYSFDNLITGS SFNEKFTYININKSVIPYDLQYDGKRSPLYGLVLANANNQMIGIITDVSYTNGVVKVR GDVTNNVINGKGTFYKTSGITFMSGDGSVKTIMIDWDGTVIAI" CDS complement(7137..8084) /note="similar to encapsidation protein of Bacillus phi29-family phages" /codon_start=1 /transl_table=11 /product="DNA encapsidation protein" /protein_id="ADR30484.1" /translation="MELTEKMIDDFLEYGAQSVVLRRLKTQTDEMQETYFDKLLESGA YDGYTFKRVHNKMYIDGKLFCTFLALNGSGVGRGGSYGKVMNIVVEEIMPEPGEHKVK REYQKLESFLATVDRFEDRIKVFCVGNNTGYYSPIFDALKLYPTLKEEGYTSNEVAVI QKAQSSNEFKETVRKTKLGRLMEITGTAKYNIDNENISNDTFNCVNKKELKETNKLKL KFRVRVDKDKVITIWECQGKEVSYYYVDNATKGLTQEYFFDFEYQKGNNKHITQLNPY TIKELELNNKLGYIYFNNPETKYLFNQINLFFKNTKGGK" CDS complement(8189..8893) /note="similar to lower collar protein of Bacillus phi29-family phages" /codon_start=1 /transl_table=11 /product="collar protein" /protein_id="ADR30485.1" /translation="MTFYKYNLELRHIDNLFNFDYPFFNEDLKPEFEKLFIKRYAFRQ IGFPTIGEFKFRLQSYLEENNDYFKQLWEIELRTKAIDFMNNKEYTETLTRDITEKEK EEIINKSLQEFMSSEISQNSGVNTTTTDTLNSNVNDGMANLGFESGLTDNTKETISEN LGSSNSTTGNNSSNTNSSDNSNRDKLFTENVVTHGVGNIGVTSASDLKKGWIEVTYSL ISKVIDGGYDLFLQRY" CDS complement(8880..9746) /note="similar to upper collar connector protein of Bacillus phi29-family phages" /codon_start=1 /transl_table=11 /product="connector protein" /protein_id="ADR30486.1" /translation="MGKKRNINKVCADRRGLLFLLYKNLATNVFKWEGLPNGIESRHI ENFLFEHGQVGFYNDENLGMICLPISNSGELNIYGDPTKFYMYSKNGNYSKTIPSDKV IRCMDNPNLVPTKSYVNYYVQEMLDIETAIRANLRKQVKPYFAIATDKNKYSVKSIID DYENGEDVVIIDKTLGEDGFDGLKLLTANVEYLVDKLRAEERSRESALLTYLGVSNVN MEKKERLITDEVNGNEEFVNLNLSIRAKGRLDAMIELQKIYPGVKCVLNMEYLEQFFK SLRSEVIGDDIL" CDS complement(9725..9889) /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ADR30487.1" /translation="MNDLVSLISNIGFPIVACLFMFNQNQKLTETITDLKVTLSGIEK RLEVWERKEI" CDS complement(9896..10546) /note="N-acetylmuramoyl-L-alanine amidase; similar to amidase-hydrolase of Clostridium phage 39-O" /codon_start=1 /transl_table=11 /product="endolysin" /protein_id="ADR30488.1" /translation="MIIGSRTGHSKKCLGAVSLRNEWECMNILEREVNKILKAHGHTI IDCNSSASTENGELSEGCRKANAQHIDIFLSYHMNASKDHKGHGTECWVHPNARASCK EIASRISSNLSKLGFYNRGVKTGLLYEMKNVNAPNIIIETCFCDNEKDIGIWSPTPYE VMARHIANALDPSIPIEEKPKRWQVRVYAFTSKEEAQKYSDRITKELKAYNVVEEI" CDS complement(10543..11565) /note="similar to tail protein of Bacillus phi29-famyli phages" /codon_start=1 /transl_table=11 /product="tail protein" /protein_id="ADR30489.1" /translation="MVNRIKDIKNGSRFFDVRPKVYNAPISLEGQRRWQNESKLHTYP YTYLTLFDGINEPFNIKPQYCKGNKMDIGVKMSLDDKLTTSIFIQNYKGDTHGRFEGL VTGANLELPTTSSNYANFISRTRSTLLQNSKNIQENFIVNNAVAENNYETARDTSILS GGVNGISNILSGNYTGLLGTGANLLSSAISANNSLSNSRMVNAMNKNMALKSLVSTVA DAKTVPNTLISKGSDIMYGLRNMEKYLKLFKFRQRDEYMEVIGDFFAMYGYAQNRLME VDTKSRQYYNYIKTKEVNILTSSIPNVYLDKLKAIYNNGTTIWHVDNPNVLPLNYSMD NTEVSE" CDS complement(11814..13442) /note="similar to lysozyme-like Gp15 protein of Clostridium phage phi3626" /codon_start=1 /transl_table=11 /product="lysozyme-peptidase" /protein_id="ADR30490.1" /translation="MATIDLLRVDQQIFNNSYEHVADFKSPSTQRYYFDNKVISSFNK DIVVQPSRISVTCPYQYRYFLDNSINYVRAFDNSRYYYYFITDIIFTAQARTELLLEL DVWQTYLWDYSVLDSIVERCHVRRWNDDGTPTKESLYADENIPTGEYIMENGNSDYNI ESLSDTVVIASTVPLGILTDSSGGEGTNPPPGEGDCGDWTNGVMSRNGFRFMKGYEGF GAYLYKDSGGVPTIGYGVTKSEPSEFDDLVARQPVSEEYASQVSYKLKQTNYGKPIVN FCKEIGITKQNQFDALCDLAFNAGVGAVVGTPTYTSLPSALRKDPFNESYIRPIWENY IISDAVGNVLNGLKARRKAECDIYFKNIYEFRPIVTINQNGSYGNPITANNGNGWLPE CSSKPEGLYVDNEVGKDWLVPVSGTISAMYPSYPSGSPHNGVDIACPVGTPVYASKDG LVRVRKELTTSYGKYLIIAHGDSDVVYAHNSQLLVNEGDNVKQGQMIARSGNSGNSSG PHLHWEIRNPNGEIIQHGVKTVNPMPGYKLNQKV" CDS complement(13447..14313) /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ADR30491.1" /translation="MKVKELLGVGYSVPVETNQVIKAMLDKVAKELFYDNHYSQGYEA ITNGGSPLGNKVNEVFFLEQKEQASMFGDLENNISGSISDSSVNIQASCLFNEHEIKR AVRNDLDLQDIYDRMLKNLRTSLLLQRRKAFLNSLTKYMNDNEIKVLKGSKLTDIASI TKLGFQVPSSDYNVSDKEIQCFDSDIICLTDFKKLGLMLNEHDTPFINWLQFNSIIEN TLPVNVNGEAVKPDMIMFDKRALKILVNDSQFTYFFNPNSLTHTITYTENIIIATNPI ANVCVLKEEGEE" CDS complement(14317..15474) /note="similar to to major head protein of Actinomyces phage Av-1" /codon_start=1 /transl_table=11 /product="head protein" /protein_id="ADR30492.1" /translation="MATGKKSNVEVTQALVNDVGGELTAELQPILDDQTMSNDQKSRA ISTKLFGYSVLKNAFIGALINKVFMSRFWSKAWENPLKMFVDGNITYGYXIEDLYVDA SDAVDYSQHFDGGTTECDDIFKLVDNKVYANYLSINFEKKFKTTIKDFDLRRAFTSEY GLGDMISQLMAKNTRGLYRLEYNLLKDMLTKYMSGISQKDVGMGTSTTPTQFIQDSQV TKVTGTTTAELIENLLLNIRIYGEQFEFESDKYNSAGVMQFSPMSDTVIVTTPQVYGH IDNYLAKTYNEDRVKILNKIILIDSLPDVNVIKSGTGTTSYVNDKPIALLMDAKLLQL KPSLLEMREIENPNALAKNYFLHFHGLVGIVPFLNCKIFTATNEQLPKLEG" CDS complement(15488..15604) /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ADR30493.1" /translation="MKLFLRVSGGEEQIEDDEEMKKPEVKQTFDDFIKDWDI" CDS complement(15667..15813) /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ADR30494.1" /translation="MNVAERPIHIQRDINISNKFLGGDNMELDEALVRLQSKTTLLYL YKKK" CDS complement(15943..16314) /codon_start=1 /transl_table=11 /product="putative DNA binding protein" /protein_id="ADR30495.1" /translation="MLNLKEQLKKDQQFGFDFMANRESGKIKDWKDEIVTITNVDLME NVTDKMTGEVKDLIVFTIKEDDNHFYFGGNAITNMFRDFFEKYGKEAIQHLVQNEGLQ VEVLGKISKTFKTEYITLEIK" repeat_region complement(16724..16748) /note="ITR" /rpt_type=inverted /rpt_type=terminal ORIGIN 1 tgggtatata acaataaaaa ataaccccta accaattccc atttacagaa attggattaa 61 gggtcatctt tagaaaggag gtgtccatca tgccaagaat ttaatttaca atataattat 121 agcatagcat tacaattatg tcaatgaaaa aataaaaata attttttaaa aaaggtattg 181 acaaggggtg gttataaatg tataataaag ttaataaatg aattaatgaa acaatggaga 241 gtgattaata atgaaaagag tttttgaaac tgcacacgca ttaactagac aatttatatc 301 tacttacggt ggagattata gagggcaatt tatcgtaaca ttaaaaatgt tatgtgaaga 361 gaagaaaaat attaaattaa gacttgaaag gttaatcgag gaaaaaaaga caaagggtat 421 aacatcaatc aatgtatatt ttaaaaatgg tgtatcactt ttcggtgatg ttgattgtat 481 agatgttaac aacaataatt ttatttttag agatttttta aaggaaaaat atattaaaac 541 aactagaaga acatcatttc atagtttaat gaaaatagat tatagattag ttgattgggt 601 atcataaaat atagtaaatt ggttggtgat agagtatgag aaaaatattt gaaggtatat 661 tattatgtgt agcatttata ttgagtgcta tatcatttat atatttatat aacaatactt 721 ttaagagtac attaattttt atattgttag tattagcaat aataatttta ttaagtaata 781 ttttatatga ttaggggtga taaattgaag cttaaagagt ttattgaaaa tttttgttat 841 aaagatacag atattaaaat ccgtggaata tcaaacaaaa gtaattctat tacatatatg 901 atatttcaag ggtacgatta cgacatagca agtgaaacgg aagtatttag cttaatagag 961 ggtttaaatg taatagacgt gaaagttgag agagaatatt tagtaataat ggtagatgat 1021 atgatttaat tttaaaatgg cacttgaaaa gtgccttttt ttattgtata ataaagttag 1081 caagtgaaag gggataaata ccatgaaata taaagtaact aaattaaacg taataaatca 1141 agaacactac gatataataa aagataaatg ctttactgaa ttatctgaaa taccattaac 1201 aactcactac gtattagata cagaaacgtg ccgtacacgt aaagaaatgc ttttagatac 1261 aaacatggag caagaggtaa aaacctatgc cgtaggtgta atgagtgtag gagataaaga 1321 caataatatg tttgtatgtg aaacgtggga aatgtttttc gagaatatct ataaaactgt 1381 acaagctaac ttaaaatttt atttaaagaa caaccaaaag agagataaaa agaatttaga 1441 cgtaactatt aaggtagggg tgcataacgt aaagtatgaa aatagcttca tgcatatgtt 1501 ttaaataatt taggctatgt atattctacc ggtgtaatag catacaacga taaaaaaggt 1561 tgctctttca ataaggttat gttagatact gggtgctata atataactca agctaacggt 1621 gtacactatg gtgctaaggt ttgtttaaaa gatactatca taaatcaaaa gataatatct 1681 aaggggaaaa ataaaggaaa attagttgag caagaaataa aaatctattt agaattttgg 1741 gatacgctaa aggttgcaca aactagccta gaaaaagctc ataagttcgt tgaaatggac 1801 gaaatgtatt acaagcttaa taaagaatat gattatgaca aagttagagc taatggtcat 1861 attttggatg ccttagagct tgagtatcta tacaacgatt tatacgcaac taagaaatta 1921 gttgaagatt tttattatga cactctttta acttttaaag gtaaggagaa aactatttat 1981 ttaaatgaca atcttaaaac atctgcttca atagctttta atacggcaat agcttatact 2041 ttcagtaatg gagaaagtga aattacagat attaaaggag ctagagagct attcaatgaa 2101 cattttgaaa tagagaaaag aattgaatta aaacaagaac ttaaagatat ggagcgttta 2161 tcataccgtg gtgggtggac tcacgcaaac ctaaaacata taaataacat agtagagaaa 2221 aatggtactt ctattgataa aaactcaagt tatccgtctc aaatgtgctt tggtttatta 2281 ccatatggag caccaatgga atttaacagt atcgaagata tgagagaata catgaaagaa 2341 aatgagctta caatggacga ccatgttttt ataataaata ctggttttga ttacgtaaga 2401 ccaaaatgca aaaaatatga tttagaagtt attcaattag gttcagtaaa tgcaactatg 2461 gaatttaaga aaatgtcttt tgaacatgaa ggaaaagtag tgcaaaatgg taacacattc 2521 ataaatagaa actactggaa cgaagacaac aaaatacaaa tatataaaaa aggttgcgaa 2581 gaaattgcaa attataattt tacaataact aacgttgaat atgatttctt aaataaatat 2641 tatgagttcg gttatcatgg agaaattgat tttatagatg atgaaatcac ttttgaaggt 2701 ttaagaactg gtaaggtatt attatataaa gccgaaaagg gtcttttaag agagtatata 2761 gaagaattat atttaatgaa agatgaagta aaaagaagaa taaacaatgg agaaaagggt 2821 cttgacgctc tttattcttc tataaaagtt cttttaaatt cattgtatgg caaattcggc 2881 tcaaaggtta gaaaaacact tgacaactta tttttaaatg atgaaggtat tttctcatgg 2941 gatataggtt gtaaaaaaga gttacgccaa aatgaaaatg aaatcgagtt actggaatat 3001 gatagtgaag aatattacgt gcctttcgct tcattcgtaa cggcttatgc gagagttgaa 3061 attcaaacaa tggtaattga agcaataggc gttgaaaact tcttatatac tgatacagat 3121 agcatttatt gctccctaac taaagagcaa ttaattgaag cacttaaaaa gattaattat 3181 gagcttgacg gttacgagct aggaaaatgg gatttagaac acgtatttaa taaattcaaa 3241 tctctagggg ctaaaaagta tatgctttat gatactagga caaaaaatat atgtgttgcg 3301 tgtgccggtg tacctaaaaa agctcaatca atactagcaa aaccaaggat ttaacgagtt 3361 ttatctaggt aaagctatcg agggaaaatt acaaagtaaa caagttaaag gtggtacgct 3421 tttaactaat atagattttg taatccaaaa taccacttat atacctcatt aaaaaatttt 3481 ttaaaaaggg ggttgtaatg ctccctatat aatgctataa taaagacatg aagggggaat 3541 aaataaaatg gctaaaagaa caacaaaaca acaatttatg aaaaaatggg ccaaggctgt 3601 taaaaataac agttatatat tcttacaaga caaagacaat atgaaaaagg cacaaaaata 3661 ttttggttta tctgacgaat atgtaaccat agttggtaac aaggcaaagg ctaattatca 3721 aatagaaaat aaaaagaaaa tcgttaatga acgtaaaact aaattacgtg atagtctttt 3781 aaaggacggt acagtcgata caactgacgt taaaaagata ataaacaacg atagacttta 3841 taagaaacta aatactagaa atgtacgctc aagcgtaaac aaagcaatcc aaactgaatt 3901 ttttaatatc atatctaaca taggtctttt taaagataat ggagcattta gaagggcgtt 3961 aaaaggaaat gacacgccaa ttaaagaatt taaaaagaag gtactcgaag ctattcaaaa 4021 atcggacaag ccttttgagt tattactttc ttttagtgaa agaatagata aggctttcga 4081 gttaatatat gaagcatatc cggaaggagc tttaaattct gtacaaggtc atagagtagt 4141 aaataaattt attgatataa tagaaactga aatgttaagg agtgtgtaat catgaaaata 4201 ataaaaacaa ctagaaatgg aaaaacaaag cacgtagtat tagaaggtga acatattaca 4261 aaggagcaat taaaggagct attaaaaact actaatggtg taagattttc atattttggt 4321 tctgaatata gaaactttca agaaattaaa gatatgctag aaatcactat cggattaaaa 4381 gagaattata ttttaataac taagagaagg ggggcgaaat tctaatgaat gaatattgta 4441 aatttaaatt taaaggtgaa atatattacg gaactattac agaagaacat gtggactact 4501 atataatcaa agttaataat aaattccctc attacttttt taaagatgac gttacaataa 4561 tttataaaat gacttacgat agataaggtg gcgtatatta tgaaaggttt aatttacact 4621 ttattattta tgttttcaat aggtggtggg gtcttaatgg ctctaccaac taatgagata 4681 gaaccacaac caataaaaga aactataaca gttgaaaagg agcttaaaga aatggagaat 4741 gaattaacac ttacaaagga atttttatta aaatcttatg aaagctcttg tgaagctttt 4801 aataaattgc atttaaatga cattaacaag tttgacgacc aagtcgcatt ggaatacctt 4861 gaggaatttg actactggtt aaatgagtta aatgaactta cagaactatt aaaaaagaac 4921 ctaccatatg aagaatttaa aattatggag tttagaaaac tttcattaat gaaagataaa 4981 gaagctcaag aggaagatat aatacaccag tacgaaggaa ctcaattatt aaacttaatg 5041 ttaaataggt ttagaagtga atacgtttat gagatatgta aaatattagt taatgaatat 5101 atgtaaaaaa gagagggtta aatcctctct ttttatatag ctataacagt tccatcccag 5161 tctatcataa tagtttttac gcttccgtca ccactcataa atgtaatacc acttgtttta 5221 taaaaagtac cttttccgtt tataacattg tttgtaacat cacctcttac ttttacgacc 5281 ccgttagtgt atgaaacgtc agttataata ccaatcattt gattatttgc gttggctaat 5341 actaaaccat aaagaggact tctttttcca tcatattgaa ggtcatatgg tataacagat 5401 ttatttatat taatataggt aaatttctcg ttaaaagaac tcccagtaat aagattatca 5461 aatgaatata attcactttt acccgtagct cctatttcaa caatagaacc ataataatca 5521 gctttttgct tccaaacaca tggattactt tgaccaccac ttacattatt acttgtttta 5581 attgcataaa cattagtatc aaggatttta acttcgtctt ttccatataa attattattc 5641 attatttcaa tgttctcaat ccacccttta agtgctacac cacaactata agaattttga 5701 tttaatggtc tccagtaatt attattaatt gttaccccat tagttattct attagttggc 5761 gtttccaagt aaagtaaacc gaaagtttct tcagaataat tattattaaa aacaattgac 5821 ccacccgtga agtttgtacc gtcattatct atatttattc tacacccgta aacaatgcta 5881 tatacgtcat tacctttatg tctttgggta tcactaggtt ctccgttttg ctctaaatcc 5941 acgttttcta tatgaatact tctacctaga gaatttattt taatgccatc tttctcattc 6001 ctattgatga atgaatcttt tatagttata aaattggttt caccagtata cccaccactt 6061 tccttaccaa tataaatacc atgacgaccg ttttgatata accaagttct attatctact 6121 atagttgcaa ttaaaccata aagcgcattt acaccatctt taccacaccc atgaatccta 6181 cagttactta aatttatcaa gtcaccaccg ttcgtttgtg atacgccgtt ttggttggtt 6241 gctcttctat tacctattat acctatatta gttgcgttta aaccgttaga aaaaatattt 6301 ccgtcatagt cagaacctat tattgaccct tgacataatg aaaaatattt cttaaaatca 6361 tttaaatctg atgaaggtct tgtataatcc catgtcataa aaggtatttc aggtgtaccc 6421 acacccacaa catataaacc tttagtattt aaactttgag tgacataata tttaccattt 6481 ggtataaata aagtattata tttattagtt ttagcgaaat taattgcgtt aataattgat 6541 tgagtactat cgtaattaac atcatctttt gcaccaaaaa atgttatatc tacaccgttt 6601 ttaattttca tttcatttac aatatttaat atctgttcac ttaaaatttt taactcattt 6661 ttagcttctt ctatctttac gttaatagtt tcaatatcac tactaacaga tgatattgaa 6721 ccttcaagct ctgtatcttt agattttaaa gtttcaatat cacctagtat ctcattatct 6781 ttagtttcta aagcttgtat tttttcgtcg tgagttgtat ctttaagttg taattgctct 6841 atagcatttt tattattctc aatgttttgt tttatctctg taagtatttg ctctgaaata 6901 agctctgcaa gagttccgtt atctttccat tgagttaata atgtaacaac ttcttgcttt 6961 aatcccacct cataaagcca ttcaagaaat gttaaggatt tattacttgc ttctatacac 7021 tcatttattt tagtaaaaaa tctgcatagt aattcattta agctcataca atcgtcaaag 7081 tcataagagt tgaatacgct acaatttaaa tcccatttgt ttaatttatt tatcatttat 7141 tttccacctt tcgtgttctt gaagaataga ttaatttggt tgaataagta tttagtttca 7201 ggattattga aataaatata acctagctta ttattaagct caagttcctt aatggtataa 7261 gggttaagtt gagtgatatg cttattgtta cctttttgat attcaaagtc gaaaaagtat 7321 tcttgagtta atcctttagt agcattatca acataataat atgaaacttc tttcccttga 7381 cattcccata ttgtaattac tttgtctttg tccactctaa ctctaaattt aagtttcaat 7441 ttattagttt ctttaagctc ctttttattg acgcaattga aagtatcatt tgagatgttt 7501 tcattatcaa tgttatattt agccgttcca gttatctcca ttaatcgacc aagtttagtt 7561 tttcttacgg tttctttaaa ttcattactt gattgagctt tttgaattac agctacctca 7621 ttacttgtat atccttcttc ttttaaagtt gggtatagtt taagagcgtc aaatattgga 7681 gaatagtacc cagtattatt acccacacaa aatactttta ttctgtcctc aaatctgtca 7741 acggttgcca agaaactttc taacttttga tattctcttt ttactttatg ctcccctggt 7801 tcaggcatta tttcctcaac gactatgttc ataacttttc catatgaccc accacgacca 7861 actccactac catttaaggc taggaaggta caaaatagtt taccgtcaat atacatttta 7921 ttatgaactc ttttaaatgt gtagccgtca tatgctccac tctcaaggag cttatcgaaa 7981 taagtttctt gcatttcatc ggtttgagtt tttaaccttc taagtacaac gctttgagca 8041 ccatactcta aaaaatcatc aatcattttt tctgtaagct ccatgactta ccatatcccc 8101 taccacttat tataatatat atcagtttat cgggatattt ctcaagtaac tcatttatct 8161 caaaatattt caatttcttt cttcctcctc aatatctctg caaaaataag tcataacccc 8221 cgtctattac tttactaatt aatgaataag ttacctcaat ccaacctttc tttaaatctg 8281 acgcacttgt tacgcctatg ttacctactc catgagttac tacattttca gtaaataatt 8341 tatccctatt tgaattatca gaactatttg tattacttga attattacca gttgttgagt 8401 tgctagagcc taaattttct gatatagttt cctttgtatt atcagttaaa ccactctcaa 8461 aacctaagtt agccataccg tcatttacat tagaatttaa agtgtcagta gttgttgtat 8521 taactccact attttgactt atttctgatg acataaattc ttgaaggctt ttatttataa 8581 tttcctcttt ttccttctct gttatatccc tagttaacgt ttctgtatat tctttattgt 8641 tcataaagtc aatggcttta gttcttaatt caatttccca tagttgttta aaataatcgt 8701 tattttcttc taggtaagat tgaagtctaa atttaaactc cccaatggtt gggaatccaa 8761 tttgcctaaa agcatatctt tttataaaga gtttttcaaa ttcaggtttt aaatcctcat 8821 taaagaatgg ataatcaaaa ttaaaaaggt tatctatatg cctaagttct aagttatatt 8881 tataaaatgt catctccaat tacctcactt cttaatgatt taaaaaattg ttctaagtat 8941 tccatattta gaacgcattt tactccggga tatattttct gtaattcaat catagcgtct 9001 aatctacctt ttgctctgat actaaggttt aaattaacaa attcctcatt cccattaacc 9061 tcatctgtta ttaatctctc tttcttttcc atgttaacat tacttacacc taaataggtt 9121 aataatgcac tttcacggct tctctcttcg gctcttagtt tatccactag atattcaaca 9181 ttagccgtta acagttttaa gccgtcaaaa ccatcttccc ctaaagtctt atctattatt 9241 acaacatctt cgccgttttc gtaatcgtca atgatacttt taacgctata tttattttta 9301 tctgtagcaa tagcgaaata aggctttacc tgttttctta aatttgctct aattgccgtc 9361 tcaatgtcaa gcatttcctg aacatagtaa ttaacatatg acttagttgg tactaggttc 9421 ggattatcca tacacctaat aactttatca ctaggaattg ttttactata gtttccattt 9481 ttagagtaca tataaaattt agttgggtcg ccataaatat ttagttcccc actatttgag 9541 attggtaaac aaatcattcc taagttttca tcattataaa aacctacttg accatgttcg 9601 aataaaaaat tctctatatg cctactttct attccattcg gtagtccttc ccatttaaaa 9661 acatttgttg ctagattttt gtacagtaag aagagaagac ctcttctatc tgcacaaact 9721 ttgtttatat ttcttttctt tcccatactt ccaacctctt ttctatacct gataatgtaa 9781 cttttaaatc tgttatagtt tcagttagtt tttgattttg gttgaacatg aataaacacg 9841 caactatagg gaaacctatg tttgatatta agctaactaa atcattcatt ttcttttata 9901 tctcctctac tacgttatac gcttttaatt cttttgttat tctatcagag tatttttgtg 9961 cttcttcttt gctagtaaat gcgtaaactc taacttgcca cctttttggt ttttcttcta 10021 ttggtattga agggtcaagg gcgtttgcta tatgtctagc cataacctca tatggtgtag 10081 gcgaccatat tccaatatct ttctcattat cacaaaaaca agtttctatt attatattag 10141 gtgcatttac attcttcatt tcatataata aaccagtttt tacacctcta ttataaaaac 10201 ctaattttga aaggttactt gaaattcttg aagctatttc tttacatgat gctcttgcgt 10261 tagggtgtac ccaacactct gtaccgtgac ccttatggtc tttgcttgcg ttcatatgat 10321 aggataaaaa tatatctatg tgttgagcgt ttgctttcct acacccttcc gaaagttccc 10381 cattttctgt acttgcggag ctattacaat ctattatggt atgcccgtga gctttaagta 10441 ttttatttac ctcacgctcc aaaatattca tacactccca ctcattacgt aagcttaccg 10501 cccctaaaca ttttttacta tgtcctgttc tacttcctat aatcattcgc tcacctctgt 10561 attatccata ctataattta atggtagtac gttcgggtta tcaacgtgcc aaattgtagt 10621 accattatta tatattgctt ttaatttatc taaataaacg tttggaattg atgatgttaa 10681 aatattaact tctttagttt taatataatt gtagtattgt cgactctttg tatcaacttc 10741 cattaatcta ttttgtgcat acccatacat ggcgaaaaag tctcctataa cttccatgta 10801 ttcgtctctt tgtctaaact taaatagctt taaatatttt tccatattac gtaaaccata 10861 cataatatca gaaccttttg agattaatgt atttggtact gttttagcgt ctgcaactgt 10921 actaactaat gattttaaag ccatgttttt attcatagca ttaaccattc ttgagttcga 10981 taaactatta ttggcactaa ttgcggagct taataaattt gctccagtac ctaatagacc 11041 cgtatagtta ccacttaaaa tattactaat accatttaca ccaccactta aaattgaagt 11101 atctcttgcc gtttcgtaat tattttctgc tacggcatta ttaacaataa aattttcttg 11161 tatatttttt gagttttgta ataatgtact tcgtgtccta gaaataaaat ttgcatagtt 11221 acttgaagta gtcggtaatt ctaagttagc acctgttact aatccttcga atctcccgtg 11281 tgtatcaccc ttatagtttt gtataaatat agaagttgta agcttatcat ctaatgacat 11341 tttaacccca atatccattt tattaccttt acaatattgt ggcttaatat tgaatggttc 11401 attaatacca tcaaataaag ttaaatatgt gtatggataa gtgtgtaatt tactttcatt 11461 ttgccacctt ctttgccctt ccaatgatat tggagcgtta tatacttttg gtcttacatc 11521 gaaaaaccta ctaccatttt ttatatcttt tattctattt accatagggt catattttaa 11581 ctccggtctt acttccgtta tctttccaaa cctttgtgta tcatatggga tacgtgtata 11641 ttctaaatcc tcaatcctaa catagggagc atagaaacag gagaaaacat tatcccctga 11701 agctcctatt acacttgacc ctgttttatc attatcaact aaatataaat ataaaccata 11761 cggtaaacca cttattttac tttcccaagt atcaacccca tattatcact cctttacact 11821 ttttggttta acttataccc aggcataggg ttaacggttt ttactccgtg ttgaattatt 11881 tcaccgtttg ggttacgtat ttcccaatgt aaatgtgggc ctgatgagtt accactatta 11941 cccgaacgtg ctatcatttg accctgtttt acgttatcac cttcgtttac caatagttgg 12001 ctattatgtg cgtaaactac atcactatcc ccatgagcta taattaaata ttttccataa 12061 cttgttgtaa gctctttacg aactctaacc aatccatctt ttgaagcgta gaccggtgta 12121 cctacgggac aagcaatatc aacgccatta tgcggagaac ctgaaggata ggaagggtac 12181 atagcactta tagttccact aacaggcact aaccaatctt taccaacttc attatccacg 12241 taaagcccct cgggtttact agaacattca ggaagccaac cattaccgtt attagccgtg 12301 attgggttac catatgaacc attttgattt atggttacta ttggtctaaa ttcgtatata 12361 ttcttaaaat atatatcaca ctctgctttt cttctagctt ttaatccatt aagtacattc 12421 cccacggcgt cacttataat ataattttcc catatagggc ggatataact ttcattgaat 12481 gggtctttac gtaatgcgct cggtaatgac gtgtaagtag gtgtaccaac taccgcaccc 12541 acaccggcat taaatgctaa atcacataaa gcatcaaatt gattttgttt agttattcca 12601 atctccttac agaaatttac gataggtttt ccgtaatttg tttgttttaa tttgtaacta 12661 acttgagaag catattcctc tgaaaccggt tgtcttgcca ctaaatcatc aaattccgaa 12721 ggctctgact tagttacacc gtaaccaatg gtaggaactc caccactatc tttgtataga 12781 tatgcaccaa atccctcata gcctttcata aacctaaaac catttcttga cattactccg 12841 tttgtccagt ctccacaatc accttcacca ggtggtgggt tagtaccctc accacccgaa 12901 ctatctgtta atattcctaa aggtacggta ctagcaatta cgaccgtatc tgatagagat 12961 tcaatgttat aatcactatt accattttcc attatatatt cacctgttgg gatattttca 13021 tcggcgtata aactctcttt tgttggtgta ccgtcatcgt tccaccttct aacatgacat 13081 ctttcgacaa tactatccaa tacggaataa tcccaaaggt aagtttgcca tacatctaat 13141 tcaagtaata gctctgttct agcttgagcc gtaaagatta tatctgttat gaaataatag 13201 taataacggg agttgtcaaa ggctctaaca taattgatac tattatctaa aaagtaccta 13261 tattgatatg gacaagtaac gcttattctt gagggttgca ctacaatatc tttattaaat 13321 gatgaaataa ctttattatc aaaatagtac ctttgagtgc taggactttt aaagtctgct 13381 acgtgttcat agctattatt aaatatttgt tggtctactc taagtaaatc tattgtagcc 13441 atttttctat tcttcccctt cttccttcaa tacacaaaca tttgcaattg gatttgttgc 13501 tatgattata ttttcagtat atgtgattgt atgagttaat gagttaggat taaagaaata 13561 agtaaattga ctgtcattaa ctaagatttt taaggctctc ttatcgaaca ttatcatatc 13621 cggttttacg gcttctccat taacatttac tggtaatgta ttctcaatga ttgagttaaa 13681 ttgaagccag ttaataaatg gtgtgtcatg ttcattaagc attaaaccaa gttttttaaa 13741 atcagttaag caaattatat cactatcaaa acattgtatc tctttatctg atacgttgta 13801 atcacttgag ggtacttgaa aacctaactt tgtaattgaa gcaatatcag ttaatttaga 13861 gccttttaat actttaatct cattatcatt catatacttt gtaagtgagt ttaaaaatgc 13921 ttttcttctt tgtaatagta atgaagttct aagatttttt aacattctat cgtaaatgtc 13981 ttgcaaatct aaatcatttc taacagctct ttttatctca tgttcgttaa ataaacatga 14041 cgcttgaata tttaccgaac tatctgaaat acttccggat atattattct ctaaatctcc 14101 aaacattgaa gcctgttctt tttgttctaa aaagaaaact tcattgactt tgttccctaa 14161 gggtgaaccc ccatttgtaa tagcttcgta accttgagaa taatggttgt cataaaatag 14221 ttccttagcc actttatcaa gcatggcttt tattacttga ttagtttcaa cgggaacgga 14281 ataaccgacc cctaatagtt cctttacttt cattttctac ccctccaatt ttggtaattg 14341 ttcattagta gccgtgaata tcttacaatt taagaacggt actataccta ctaaaccgtg 14401 gaaatgtaag aagtaatttt tcgctaatgc gtttggattt tcaatctctc tcatttctaa 14461 taatgaaggt tttaattgta ataatttagc gtccattaat agagctattg gtttatcatt 14521 tacgtagcta gtagttccag taccgctctt aattacattt acatcaggta aactatctat 14581 aaggattatt ttatttaata tttttactct atcctcattg taagttttcg ctaagtagtt 14641 atctatatga ccatatactt gaggagtagt aactattacc gtatcactca tagggctaaa 14701 ttgcattacg ccggcactat tgtatttatc actttcaaac tcgaattgct ctccgtaaat 14761 tctaatattt aaaagtagat tttcaataag ctctgccgta gttgttccag taacttttgt 14821 tacttgactg tcttgtataa attgagttgg tgtagtactt gtacccattc ccacatcttt 14881 ttgtgatata ccactcatat actttgtaag catgtctttt aataagttat actctaatct 14941 atatagaccc cttgtattct tagccattaa ttgacttatc atatcaccta gaccgtactc 15001 acttgtaaac gcccttctta aatcaaagtc cttaatagta gtcttaaatt tcttttcaaa 15061 gttgatagat aaatagtttg cgtaaacctt gttgtctact aatttgaata tatcatcaca 15121 ttcagttgtg ccaccgtcaa agtgttgtga ataatcaaca gcgtcagaag cgtccacgta 15181 taaatcctcg atantataac cataagtgat gttaccgtca acaaacatct ttaaaggatt 15241 ttcccacgct ttactccaaa atctagacat aaatacttta ttaattaatg cacctatgaa 15301 cgcatttttt aatacactat atccgaataa cttcgttgat attgctctac tcttttggtc 15361 gtttgacatt gtttggtcgt ctaatattgg ttgtaattct gctgtaagtt cgcccccaac 15421 atcatttact aacgcttgag taacttcgac attcgatttc tttccagttg ccatttacat 15481 attcccccta tatatcccag tcttttataa agtcgtcaaa agtttgtttt acttcgggtt 15541 tcttcatttc ttcatcatct tcgatttgtt cttctccacc cgatactctt aaaaataact 15601 tcatgttatt ttctcttagc ctttgcgttt ctttttcttt ttcttgtaag gcttctttat 15661 ttcttcttat ttcttcttgt aaagatacaa tagtgtcgtt ttgctctgta atcttactag 15721 agcttcatct agctccatat tatcaccacc taaaaattta ttagagatat tgatatccct 15781 ttgtatatgt ataggtcgtt ccgcaacatt caaacgtcaa tagtgaaggc ggattctacc 15841 ccgtgcgtac ccttcaatct atacctatac acataaaaag agatacccta attggatatc 15901 tctgttttat taaggtacta cgtgaacttt atatattaaa ttttatttta tctctaatgt 15961 tatatattct gtcttgaatg tttttgatat cttacctaat acttcaactt gtaagccttc 16021 gttttgtact aagtgttgaa tagcttcttt accgtatttc tcaaagaaat ctctaaacat 16081 atttgttata gcgttaccac caaaataaaa gtgattgtcg tcttctttga tagtgaatac 16141 tattaagtct ttaacttctc cagtcatttt atctgttaca ttctccatta aatcaacgtt 16201 tgtgattgta acaatttcgt ctttccaatc cttaatttta ccactctctc tgttagccat 16261 aaaatcaaat ccaaattgtt ggtctttctt taattgttct tttaagttta gcattttaca 16321 ttacctcttt ctttcattta aattttttgc attttacttt tggaagtgtt ttcccttccg 16381 atactttaat tatactctag tcttatgagt ttgtcaagac ttttttacta gagttttgtt 16441 agaattttta atacattttg tacaagatat gtgcgtttat atgttttaaa tttaattttg 16501 taaggtgttc cttacattta ttattatact aaagtttata acatttgtaa agtgttttct 16561 ttaaaatttt tattttttta tttttaatat ttttattgac atggttgtac tattatgtta 16621 caataatatt gtagttagtt actgtttatt gtttttcatg aattgaatac ctccccttaa 16681 aaatagcttc tattgaaatt ttctaaatgg gaatttcata ggagttattt tttattgtta 16741 tataccca //