LOCUS EF380019 5386 bp ss-DNA circular PHG 19-FEB-2007 DEFINITION Enterobacteria phage phiX174 isolate 100A90, complete genome. ACCESSION EF380019 VERSION EF380019.1 KEYWORDS . SOURCE Escherichia virus phiX174 ORGANISM Escherichia virus phiX174 Viruses; ssDNA viruses; Microviridae; Bullavirinae; Phix174microvirus. REFERENCE 1 (bases 1 to 5386) AUTHORS Silander,O.K., Tenaillon,O. and Chao,L. TITLE Understanding the Evolutionary Fate of Finite Populations: The Dynamics of Mutational Effects JOURNAL Unpublished REFERENCE 2 (bases 1 to 5386) AUTHORS Silander,O.K., Tenaillon,O. and Chao,L. TITLE Direct Submission JOURNAL Submitted (23-JAN-2007) Division of Biology, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA FEATURES Location/Qualifiers source 1..5386 /organism="Escherichia virus phiX174" /mol_type="genomic DNA" /isolate="100A90" /host="Escherichia coli" /db_xref="taxon:10847" CDS join(3981..5386,1..136) /note="viral strand synthesis" /codon_start=1 /transl_table=11 /product="protein A" /protein_id="ABN49732.1" /translation="MVRSYYLSECHADYFDFERIEALKPAIEACGISTLSQSPMLGFH KQMDNRIKLLEEILSFRMQGVEFDNGDMYVDGHKAASDVRDEFVFVTEKLMDELAQCY NVLPQLDINNTIDHRPEGDEKWFLENEKTVTQFCRKLAAERPLKDIRDEYNYPKKKGI KDECSRLLEASTMKSRRGFTIQRLMNAMRQAHADGWFIVFDTLTLADDRLEAFYDNPN ALRDYFRDIGRMVLAAEGRKANDSHADCYQYFCVPEYGTANGRLHFHAVHFMRTLPTG SVDPNFGRRVRNRRQLNSLQNTWPYGYSMPIAVRYTQDAFSRSGWLWPVDAKGEPLKA TSYMAVGFYVAKYVNKKSDMDLAAKGLGAKEWNNSLKTKLSLLPKKLFRIRMSRNFGM KMLTMTNLSTECLIQLTKLGYDATPFNQILKQNAKREMRLRLGKVTVADVLAAQPVTT NLLKFMRVSIKMIGVSNLQSFIASMTQKLTLSDISDESKNYLDKAGITTACLRIKSKW TAGGK" CDS join(4497..5386,1..136) /note="shuts off host DNA synthesis" /codon_start=1 /transl_table=11 /product="protein A*" /protein_id="ABN49733.1" /translation="MKSRRGFTIQRLMNAMRQAHADGWFIVFDTLTLADDRLEAFYDN PNALRDYFRDIGRMVLAAEGRKANDSHADCYQYFCVPEYGTANGRLHFHAVHFMRTLP TGSVDPNFGRRVRNRRQLNSLQNTWPYGYSMPIAVRYTQDAFSRSGWLWPVDAKGEPL KATSYMAVGFYVAKYVNKKSDMDLAAKGLGAKEWNNSLKTKLSLLPKKLFRIRMSRNF GMKMLTMTNLSTECLIQLTKLGYDATPFNQILKQNAKREMRLRLGKVTVADVLAAQPV TTNLLKFMRVSIKMIGVSNLQSFIASMTQKLTLSDISDESKNYLDKAGITTACLRIKS KWTAGGK" CDS join(5075..5386,1..51) /note="capsid morphogenesis" /codon_start=1 /transl_table=11 /product="protein B" /protein_id="ABN49734.1" /translation="MEQFTKNQAVATSQEAVQNQNEPQLRDENAHNDKSVHGVLNPTY QAGLRRDAVQSDIEAECKKRDEIEAGKSYCSRRFGGATCDDKSAQIYARFDKNDWRIQ PAEFYRFHDAEVNTFGYF" CDS 51..221 /codon_start=1 /transl_table=11 /product="protein K" /protein_id="ABN49735.1" /translation="MSRKIILIKQELLLLVYELNRSGLLAENEKIRPILAQFEKLLLC DLSPSTNDSVKN" CDS 133..369 /note="DNA maturation" /codon_start=1 /transl_table=11 /product="protein C" /protein_id="ABN49736.1" /translation="MRKFDLSLRSSRSSYFATFRHQLTILSKTDALDEEKWLNMLGTF VKDWFRYESHFVHGRDSLVDILKERGLLSESDAV" CDS 390..848 /note="capsid morphogenesis" /codon_start=1 /transl_table=11 /product="protein D" /protein_id="ABN49737.1" /translation="MSQVTEQSVRFQTALASIKLIQASAVLDLTEDDFDFLTSNKVWI ATDRSRARRCVEACVYGTLDFVGYPRFPAPVEFIAAVIAYYVHPVNIQTACLIMEGAE FTENIINGVERPVKAAELFAFTLRVRAGNTDVLTDAEENVRQKLRAEGVM" CDS 568..843 /note="cell lysis protein" /codon_start=1 /transl_table=11 /product="protein E" /protein_id="ABN49738.1" /translation="MVRWTLWDTLAFLLLLSLLLPSLLIMFIPSTFKRPVSSWKALNL RKTLLMASSVRLKPLNCSRLPCVYAQETLTFLLTQKKTCVKNYVQKE" CDS 848..964 /note="DNA condensation" /codon_start=1 /transl_table=11 /product="protein J" /protein_id="ABN49739.1" /translation="MSKGKKRSGARSGRPQPLRGTKGKRKGARLWYVGGQQF" CDS 1001..2284 /note="major coat protein" /codon_start=1 /transl_table=11 /product="protein F" /protein_id="ABN49740.1" /translation="MSNIQTGAERMPHDLSHLGFLAGQIGRLITISTTPVIAGDSFEM DAVGALRLSPLRRGLAIDSTVDIFTFYVPHRHVYGEQWIKFMKDGVNATPLPTVNTTG YIDHAAFLGTINPDTNKIPKHLFQGYLNIYNNYFKAPWMPDRTEANPNELNEDDARHG FRCCHLKNIWTAPLPPETELSRQMTTSTTSIDIMGLQAAYANLHTDQERDYFMQRYRD VISSFGGKTSYDADNRPLLVMRSNFWASGYDVDGTDQTSLGQFSGRVQQTYKHSVPRF FVPEHGTMFTLALVRFPPTATKEIQYLNAKGALTYTDIAGDPILYGNLPPREISMKDV FRSGDLSKKFKIAEGQWYRYAPSYVSPVYHLLEGFPFIQEPPSGDLQERVLIRHHDYD QCFQSVQLLQWNSQVKFNVTVYRNLPTTRDSIMTS" CDS 2395..2922 /note="major spike protein" /codon_start=1 /transl_table=11 /product="protein G" /protein_id="ABN49741.1" /translation="MFQTFISRHNSNFFSDKLVLTSVTPASSAPVLQTPKATSSTLYF DSLTVNAGNGGFLHCIQMDTSVNAANQVVSVGADIAFDADPKFFACLVRFESSSVPTT LPTAYDVYPLDGRHDGGYYTVKDCVTIDVLPRTSGNNVYVGFMVWSNFTATKCRGLVS LNQVIKEIICLQPLK" CDS 2931..3917 /note="minor spike protein" /codon_start=1 /transl_table=11 /product="protein H" /protein_id="ABN49742.1" /translation="MFGAIAGGIASALAGGAMSKLFGGGQKAASGGIQGDVLATDNNT VGMGDAGIKSAIQGSNVPNHDEAAPNFVSGAMAKVGKGLLEGALQAGTSAVSDKLLDL VGLGGKSAADKGKDTRDYLAAAFPELNAWERAGANASSAGMVDAGFENQKELTKMQLD NQKEIAEMQNETQKEIAGIQSATSRQNTKDQVYAQNEMLAYQQKESTARVASIMENTN LSKQQQVSEIMRQMLTQAQTAGQYFTNDQIKEMTRKVSAEVDLVHQQTQNQRYGSSHI GATAKDISNVVTDAASGVVDIFHGIDKAVADTWNNFWKDGKADGIGSNLSRK" ORIGIN 1 gagttttatc gcttccatga cgcagaagtt aacactttcg gatatttctg atgagtcgaa 61 aaattatctt gataaagcag gaattactac tgcttgttta cgaattaaat cgaagtggac 121 tgctggcgga aaatgagaaa attcgaccta tccttgcgca gttcgagaag ctcttacttt 181 gcgacctttc gccatcaact aacgattctg tcaaaaactg acgcgttgga tgaggagaag 241 tggcttaata tgcttggcac gttcgtcaag gactggttta gatatgagtc acattttgtt 301 catggtagag attctcttgt tgacatttta aaagagcgtg gattactatc tgagtccgat 361 gctgtttaac tactaatagg taagaaatca tgagtcaagt tactgaacaa tccgtacgtt 421 tccagaccgc tttggcctct attaagctca ttcaggcttc tgccgttttg gatttaaccg 481 aagatgattt cgattttctg acgagtaaca aagtttggat tgctactgac cgctctcgtg 541 ctcgtcgctg cgttgaggct tgcgtttatg gtacgctgga ctttgtggga taccctcgct 601 ttcctgctcc tgttgagttt attgctgccg tcattgctta ttatgttcat cccgtcaaca 661 ttcaaacggc ctgtctcatc atggaaggcg ctgaatttac ggaaaacatt attaatggcg 721 tcgagcgtcc ggttaaagcc gctgaattgt tcgcgtttac cttgcgtgta cgcgcaggaa 781 acactgacgt tcttactgac gcagaagaaa acgtgcgtca aaaattacgt gcagaaggag 841 tgatgtaatg tctaaaggta aaaaacgttc tggcgctcgc tctggtcgtc cgcagccgtt 901 gcgaggtact aaaggcaagc gtaaaggcgc tcgtctttgg tatgtaggtg gtcaacaatt 961 ttaattgcag gggcttcggc cccttacttg aggataaatt atgtctaata ttcaaactgg 1021 cgccgagcgt atgccgcatg acctttccca tcttggcttc cttgctggtc agattggtcg 1081 tcttattacc atttcaacta ctccggttat cgctggcgac tccttcgaga tggacgccgt 1141 tggcgctctc cgtctttctc cattgcgtcg tggccttgct attgactcta ctgtagacat 1201 ttttactttt tatgtccctc atcgtcacgt ttatggtgaa cagtggatta agttcatgaa 1261 ggatggtgtt aatgccactc ctctcccgac tgttaacact actggttata ttgaccatgc 1321 cgcttttctt ggcacgatta accctgatac caataaaatc cctaagcatt tgtttcaggg 1381 ttatttgaat atctataaca actattttaa agcgccgtgg atgcctgacc gtaccgaggc 1441 taaccctaat gagcttaatg aagatgatgc tcgtcatggt ttccgttgct gccatctcaa 1501 aaacatttgg actgctccgc ttcctcctga gactgagctt tctcgccaaa tgacgacttc 1561 taccacatct attgacatta tgggtctgca agctgcttat gctaatttgc atactgacca 1621 agaacgtgat tactttatgc agcgttaccg tgatgttatt tcttcatttg gaggtaaaac 1681 ctcttatgac gctgacaacc gtcctttact tgtcatgcgc tctaatttct gggcatctgg 1741 ctatgatgtt gatggaactg accaaacgtc gttaggccag ttttctggtc gtgttcaaca 1801 gacctataaa cattctgtgc cgcgtttctt tgttcctgag catggtacta tgtttactct 1861 tgcgcttgtt cgttttccgc ctactgcgac taaagagatt cagtacctta acgctaaagg 1921 tgctttgact tataccgata ttgctggcga ccctattttg tatggcaact tgccgccgcg 1981 tgaaatttct atgaaggatg ttttccgttc tggtgatttg tctaagaagt ttaagattgc 2041 tgagggtcag tggtatcgtt atgcgccttc gtatgtttct cctgtttatc accttcttga 2101 aggcttccca ttcattcagg aaccgccttc tggtgatttg caagaacgcg tacttattcg 2161 ccaccatgat tatgaccagt gtttccagtc cgttcagttg ttgcagtgga atagtcaggt 2221 taaatttaat gtgaccgttt atcgcaatct gccgaccact cgcgattcaa tcatgacttc 2281 gtgataaaag attgagtgtg aggttataac gtcgaagcgg taaaaatttt aatttttgcc 2341 gctgaggggt tgaccaagcg aagcgcggta ggttttttgc ttaggagttt aatcatgttt 2401 cagactttta tttctcgcca taattcaaac tttttttctg ataagctggt tctcacttct 2461 gttactccag cttcttcggc acctgtttta cagacaccta aagctacatc gtcaacgtta 2521 tattttgata gtttgacggt taatgctggt aatggtggtt ttcttcattg cattcagatg 2581 gatacatctg tcaacgccgc taatcaggtt gtttctgttg gtgctgatat tgcttttgat 2641 gccgacccta aattttttgc ctgtttggtt cgttttgagt cttcttcggt tccgactacc 2701 ctcccgactg cttatgatgt ttatcctttg gatggtcgcc atgatggtgg ttattatacc 2761 gtcaaggact gtgtgactat tgacgtcctt ccccgtacgt cgggcaataa tgtttatgtt 2821 ggtttcatgg tttggtctaa ctttaccgct actaaatgcc gcggattggt ttcgctgaat 2881 caggttatta aagagattat ttgtctccag ccacttaagt gaggtgattt atgtttggtg 2941 ctattgctgg cggtattgct tctgctcttg ctggtggcgc catgtctaaa ttgtttggag 3001 gcggtcaaaa agccgcctcc ggtggcattc aaggtgatgt gcttgctacc gataacaata 3061 ctgtaggcat gggtgatgct ggtattaaat ctgccattca aggctctaat gttcctaacc 3121 atgatgaggc cgcccctaat tttgtttctg gtgctatggc taaagttggt aaaggacttc 3181 ttgaaggtgc gttgcaggct ggcacttctg ccgtttctga taagttgctt gatttggttg 3241 gacttggtgg caagtctgcc gctgataaag gaaaggatac tcgtgattat cttgctgctg 3301 catttcctga gcttaatgct tgggagcgtg ctggtgctaa tgcttcctct gctggtatgg 3361 ttgacgccgg atttgagaat caaaaagagc ttactaaaat gcaactggac aatcagaaag 3421 agattgccga gatgcaaaat gagactcaaa aagagattgc tggcattcag tcggcgactt 3481 cacgccagaa tacgaaagac caggtatatg cacaaaatga gatgcttgct tatcaacaga 3541 aggagtctac tgctcgcgtt gcgtctatta tggaaaacac caatctttcc aagcaacagc 3601 aggtttccga gattatgcgc caaatgctta ctcaagctca aacggctggt cagtatttta 3661 ccaatgacca aatcaaagaa atgactcgca aggttagtgc tgaggttgac ttagttcatc 3721 agcaaacgca gaatcagcgg tatggctctt ctcatattgg cgctactgca aaggatattt 3781 ctaatgtcgt cactgatgct gcttctggtg tggttgatat ttttcatggt attgataaag 3841 ctgttgccga tacttggaac aatttctgga aagacggtaa agctgatggt attggctcta 3901 atttgtctag gaaataaccg tcaggattga caccctccca attgtatgtt ttcatgcctc 3961 caaatcttgg aggctttttt atggttcgtt cttattacct ttctgaatgt cacgctgatt 4021 attttgactt tgagcgtatc gaggctctta aacctgctat tgaggcttgt ggcatttcta 4081 ctctttctca atccccaatg cttggcttcc ataagcagat ggataaccgc atcaagctct 4141 tggaagagat tttgtctttt cgtatgcagg gcgttgagtt tgataatggt gatatgtatg 4201 ttgacggcca taaggctgct tctgacgttc gtgatgagtt tgtatttgtt actgagaagt 4261 taatggatga attggcacaa tgctacaatg tgctccccca acttgatatt aataacacta 4321 tagaccaccg ccccgaaggg gacgaaaaat ggtttttaga gaacgagaag acggttacgc 4381 agttttgccg caagctggct gctgaacgcc ctcttaagga tattcgcgat gagtataatt 4441 accccaaaaa gaaaggtatt aaggatgagt gttcaagatt gctggaggcc tccactatga 4501 aatcgcgtag aggctttact attcagcgtt tgatgaatgc aatgcgacag gctcatgctg 4561 atggttggtt tatcgttttt gacactctca cgttggctga cgaccgatta gaggcgtttt 4621 atgataatcc caatgctttg cgtgactatt ttcgtgatat tggtcgtatg gttcttgctg 4681 ccgagggtcg caaggctaat gattcacacg ccgactgcta tcagtatttt tgtgtgcctg 4741 agtatggtac agctaatggc cgtcttcatt tccatgcggt gcactttatg cggacacttc 4801 ctacaggtag cgttgaccct aattttggtc gtcgggtacg caatcgccgc cagttaaata 4861 gcttgcaaaa tacgtggcct tatggttaca gtatgcctat cgcagttcgc tacacgcagg 4921 acgctttttc acgttctggt tggttgtggc ctgttgatgc taaaggtgag ccgcttaaag 4981 ctactagtta tatggctgtt ggtttctatg tggctaaata cgttaacaaa aagtcagata 5041 tggaccttgc tgctaaaggt ctaggagcta aagaatggaa caattcacta aaaaccaagc 5101 tgtcgctact tcccaagaag ctgttcagaa tcagaatgag ccgcaacttc gggatgaaaa 5161 tgctcacaat gacaaatctg tccacggagt gcttaatcca acttaccaag ctgggttacg 5221 acgcgacgcc gttcaatcag atattgaagc agaatgcaaa aagagagatg agattgaggc 5281 tgggaaaagt tactgtagcc gacgttttgg cggcgcaacc tgtgacgaca aatctgctca 5341 aatttatgcg cgtttcgata aaaatgattg gcgtatccaa cctgca //