LOCUS EF380020 5386 bp ss-DNA circular PHG 19-FEB-2007 DEFINITION Enterobacteria phage phiX174 isolate 100B90, complete genome. ACCESSION EF380020 VERSION EF380020.1 KEYWORDS . SOURCE Escherichia virus phiX174 ORGANISM Escherichia virus phiX174 Viruses; ssDNA viruses; Microviridae; Bullavirinae; Phix174microvirus. REFERENCE 1 (bases 1 to 5386) AUTHORS Silander,O.K., Tenaillon,O. and Chao,L. TITLE Understanding the Evolutionary Fate of Finite Populations: The Dynamics of Mutational Effects JOURNAL Unpublished REFERENCE 2 (bases 1 to 5386) AUTHORS Silander,O.K., Tenaillon,O. and Chao,L. TITLE Direct Submission JOURNAL Submitted (23-JAN-2007) Division of Biology, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA FEATURES Location/Qualifiers source 1..5386 /organism="Escherichia virus phiX174" /mol_type="genomic DNA" /isolate="100B90" /host="Escherichia coli" /db_xref="taxon:10847" CDS join(3981..5386,1..136) /note="viral strand synthesis" /codon_start=1 /transl_table=11 /product="protein A" /protein_id="ABN49743.1" /translation="MVRSYYLSECHADYFDFERIEALKPVIEACGISTLSQSPMLGFH KQMDNRIKLLEEILSFRMQGVEFDNGDMYVDGHKAAFDVRDEFVSVTEKLMDELAQCY NVLPQLDINNTIDHCPEGDEKWFLENEKTVTQFCRKLAAERPLKDIRDEYNYPKKKGI KDECSRLLEASTMKSRRGFTIQRLMNAMRQAHADGWFIVFDTLTLADDRLEAFYDNPN ALRDYFRDIGRMVLAAEGRKANDSHADCYQYFCVPEYGTANGRLHFHAVHFMRTLPTG SVDPNFGRRVRNRRQLNSLQNTWPYGYSMPIAVRFTQDAFSRSGWLWPVDAKGEPLKA TSYMAVGFYVAKYVNKKSDMDLAAKGLGAKEWNNSLKTKLSLLPKKLFRIRMSRNFGM KMLTMTNLSTECLIQLTKLGYDATPFNQILKQNAKREMRLRLGKVTVADVLAAQPVTT NLLKFMRASIKMIGVSNLQSFIASMTQKLTLSDISDESKNYLDKAGIITACLRIKSKW TAGGK" CDS join(4497..5386,1..136) /note="shuts off host DNA synthesis" /codon_start=1 /transl_table=11 /product="protein A*" /protein_id="ABN49744.1" /translation="MKSRRGFTIQRLMNAMRQAHADGWFIVFDTLTLADDRLEAFYDN PNALRDYFRDIGRMVLAAEGRKANDSHADCYQYFCVPEYGTANGRLHFHAVHFMRTLP TGSVDPNFGRRVRNRRQLNSLQNTWPYGYSMPIAVRFTQDAFSRSGWLWPVDAKGEPL KATSYMAVGFYVAKYVNKKSDMDLAAKGLGAKEWNNSLKTKLSLLPKKLFRIRMSRNF GMKMLTMTNLSTECLIQLTKLGYDATPFNQILKQNAKREMRLRLGKVTVADVLAAQPV TTNLLKFMRASIKMIGVSNLQSFIASMTQKLTLSDISDESKNYLDKAGIITACLRIKS KWTAGGK" CDS join(5075..5386,1..51) /note="capsid morphogenesis" /codon_start=1 /transl_table=11 /product="protein B" /protein_id="ABN49745.1" /translation="MEQLTKNQAVATSQEAVQNQNEPQLRDENAHNDKSVHGVLNPTY QAGLRRDAVQPDIEAERKKRDEIEAGKSYCSRRFGGATCDDKSAQIYARFDKNDWRIQ PAEFYRFHDAEVNTFGYF" CDS 51..221 /codon_start=1 /transl_table=11 /product="protein K" /protein_id="ABN49746.1" /translation="MSRKIILIKQELLLLVYELNRSGLLAENEKIRPILAQLEKLLLC DLSPSTNDSVKN" CDS 133..369 /note="DNA maturation" /codon_start=1 /transl_table=11 /product="protein C" /protein_id="ABN49747.1" /translation="MRKFDLSLRSSRSSYFATFRHQLTILSKTDALDEEKWLNMLGTF VKDWFRYESHFVHGRDSLVDILKERGLLSESDAV" CDS 390..848 /note="capsid morphogenesis" /codon_start=1 /transl_table=11 /product="protein D" /protein_id="ABN49748.1" /translation="MSQVIEQSVRFQTALASIKLIQASAVLDLTEDDFDFLTSNKVWI ATDRSRARRCVEACVYGTLDFVGYPRFPAPVEFIAAVIAYYVHPVNIQTACLIMEGAE FTENIINGVERPVKAAELFAFTLRVRAGNIDVLTDAEENVRQKLRAEGVM" CDS 568..843 /note="cell lysis protein" /codon_start=1 /transl_table=11 /product="protein E" /protein_id="ABN49749.1" /translation="MVRWTLWDTLAFLLLLSLLLPSLLIMFIPSTFKRPVSSWKALNL RKTLLMASSVRLKPLNCSRLPCVYAQETLTFLLTQKKTCVKNYVQKE" CDS 848..964 /note="DNA condensation" /codon_start=1 /transl_table=11 /product="protein J" /protein_id="ABN49750.1" /translation="MSKGKKRSGARPGRPQPLRGTKGKRKGVRLWYVGGQQF" CDS 1001..2284 /note="major coat protein" /codon_start=1 /transl_table=11 /product="protein F" /protein_id="ABN49751.1" /translation="MSNIQTGAERMPHDLSHLGFLAGQIGRLITISTTPVIAGDSFEM DAVGALRLSPLRRGLAIDSTVDIFTFYVPHRHVYGEQWIKFMKDGVNATPLPTVNTAG YIDHAAFLGTINPDTNKIPKHLFQGYLNIYNNYFKAPWMPGRTEANPNELNEDDARYG FRCCHLKNIWTAPLPPETELSRQMTTSTTSIDIMGLQAAYANLHTDQERDYFMQRYRD VISSFGGKTSYDADNRPLLVMRSNFWASGYDVDGTDQTSLGQFSGRVQQTYKHSVPRF FVPEHGTMFTLALVRFPPTATKEIQYLNAKGALTYTDIAGDPVLYGNLPPREISMKDV FRSGDSSKKFKIAEGQWYRYAPSYVSPVYHLLEGFPFIQEPPSGDLQERVLIHHHDYD QCFQSVQLLQWNSQVKFNVTVYRNLPTTRDSIMTS" CDS 2395..2922 /note="major spike protein" /codon_start=1 /transl_table=11 /product="protein G" /protein_id="ABN49752.1" /translation="MFQTFISRHNSNFFSDKLVLTSVTPASSAPVLQTPKATSSTLYF DSLTVNAGNGGFLHCIQMDTSVNAANQVVSVGVDIAFDADPKFFACLVRFESSSVPTI LPTAYDVYPLDGRHDGGYYTVKDCVTIDVLPRTPGNNVYVGFMVWSNFTATKCRGLVS LNQVIKEIICLQPLK" CDS 2931..3917 /note="minor spike protein" /codon_start=1 /transl_table=11 /product="protein H" /protein_id="ABN49753.1" /translation="MFGAIAGGIASALAGGAMSKLFGGGQKAASGGIQGDVLATDNNT VGMGDAGIKSAIQGSNVPNHDEAAPNFVSGAMAKAGKGLLEGALQAGTSAVSDKLLDL VGLGGKSAADKGKDTRDYLAAAFPELNAWERAGANASSAGMVDAGFENQKELTKMQLD NQKEIAEMQNETQKEIAGIQSATSRQNTKDQVYAQNEMLAYQQKESTARVASIMENTN LSKQQQVSEIMRQMLTQAQTAGQYFTNDQIKEMTRKVSAEVDLVHQQTQNQRYGSSHI GATAKDISNVVTDAASGVVDIFHGIDKAVADTWNNFWKDGKADGIGSNLSRK" ORIGIN 1 gagttttatc gcttccatga cgcagaagtt aacactttcg gatatttctg atgagtcgaa 61 aaattatctt gataaagcag gaattattac tgcttgttta cgaattaaat cgaagtggac 121 tgctggcgga aaatgagaaa attcgaccta tccttgcgca gctcgagaag ctcttacttt 181 gcgacctttc gccatcaact aacgattctg tcaaaaactg acgcgttgga tgaggagaag 241 tggcttaata tgcttggcac gttcgtcaag gactggttta gatatgagtc acattttgtt 301 catggtagag attctcttgt tgacatttta aaagagcgtg gattactatc tgagtccgat 361 gctgtttaac cactaatagg taagaaatca tgagtcaagt tattgaacaa tccgtacgtt 421 tccagaccgc tttggcctct attaagctca ttcaggcttc tgccgttttg gatttaaccg 481 aagatgattt cgattttctg acgagtaaca aagtttggat tgctactgac cgctctcgtg 541 ctcgtcgctg cgttgaggct tgcgtttatg gtacgctgga ctttgtggga taccctcgct 601 ttcctgctcc tgttgagttt attgctgccg tcattgctta ttatgttcat cccgtcaaca 661 ttcaaacggc ctgtctcatc atggaaggcg ctgaatttac ggaaaacatt attaatggcg 721 tcgagcgtcc ggttaaagcc gctgaattgt tcgcgtttac cttgcgtgta cgcgcaggaa 781 acattgacgt tcttactgac gcagaagaaa acgtgcgtca aaaattacgt gcagaaggag 841 tgatgtaatg tctaaaggta aaaaacgttc tggcgctcgc cctggtcgtc cgcagccgtt 901 gcgaggtact aaaggcaagc gtaaaggcgt tcgtctttgg tatgtaggtg gtcaacaatt 961 ttaattgcag gggcttcggc cccttacttg aggataaatt atgtctaata ttcaaactgg 1021 cgccgagcgt atgccgcatg acctttccca tcttggcttc cttgctggtc agattggtcg 1081 tcttattacc atttcaacta ctccggttat cgctggcgac tccttcgaga tggacgccgt 1141 tggtgctctc cgtctttctc cattgcgtcg tggccttgct attgactcta ctgtagacat 1201 ttttactttt tatgtccctc atcgtcacgt ttatggtgaa cagtggatta agttcatgaa 1261 ggatggtgtt aatgccactc ctctcccgac tgttaacact gctggttata ttgaccatgc 1321 cgcttttctt ggcacgatta accctgatac caataaaatc cctaagcatt tgtttcaggg 1381 ttatttgaat atctataaca actattttaa agcgccgtgg atgcctggcc gtaccgaggc 1441 taaccctaat gagcttaatg aagatgatgc tcgttatggt ttccgttgct gccatctcaa 1501 aaacatttgg actgctccgc ttcctcctga gactgagctt tctcgccaaa tgacgacttc 1561 taccacatct attgacatta tgggtctgca agctgcttat gctaatttgc atactgacca 1621 agaacgtgat tacttcatgc agcgttaccg tgatgttatt tcttcatttg gaggtaaaac 1681 ctcttatgac gctgacaacc gtcctttact tgtcatgcgc tctaatttct gggcatctgg 1741 ctatgatgtt gatggaactg accaaacgtc gttaggccag ttttctggtc gtgttcaaca 1801 gacctataaa cattctgtgc cgcgtttctt tgttcctgag catggcacta tgtttactct 1861 tgcgcttgtt cgttttccgc ctactgcgac taaagagatt cagtacctta acgctaaagg 1921 tgctttgact tataccgata ttgctggcga ccctgttttg tatggcaact tgccgccgcg 1981 tgaaatttct atgaaggatg ttttccgttc tggtgattcg tctaagaagt ttaagattgc 2041 tgagggtcag tggtatcgtt atgcgccttc gtatgtttct cctgtttatc accttcttga 2101 aggcttccca ttcattcagg aaccgccttc tggtgatttg caagaacgcg tacttattca 2161 tcatcatgat tatgaccagt gtttccagtc cgttcagttg ttgcagtgga atagtcaggt 2221 taaatttaat gtgaccgttt atcgcaatct gccgaccact cgcgattcaa tcatgacttc 2281 gtgataaaag attgagtgtg aggttataac gccgaagcgg taaaaatttt aatttttgcc 2341 gctgaggggt tgaccaagcg aagcgcggta ggttttctgc ttaggagttt aatcatgttt 2401 cagactttta tttctcgcca taattcaaac tttttttctg ataagctggt tctcacttct 2461 gttactccag cttcttcggc acctgtttta cagacaccta aagctacatc gtcaacgtta 2521 tattttgata gtttgacggt taatgctggt aatggtggtt ttcttcattg cattcagatg 2581 gatacatctg tcaacgccgc taatcaggtt gtttctgttg gtgttgatat tgcttttgat 2641 gccgacccta aattttttgc ctgtttggtt cgctttgagt cttcttcggt tccgactatc 2701 ctcccgactg cttatgatgt ttatcctttg gatggtcgcc atgatggtgg ttattatacc 2761 gtcaaggact gtgtgactat tgacgtcctt ccccgtacgc cgggcaataa tgtttatgtt 2821 ggtttcatgg tttggtctaa ctttaccgct actaaatgcc gcggattggt ttcgctgaat 2881 caggttatta aagagattat ttgtctccag ccacttaagt gaggtgattt atgtttggtg 2941 ctattgctgg cggtattgct tctgctcttg ctggtggcgc catgtctaaa ttgtttggag 3001 gcggtcaaaa agccgcctcc ggtggcattc aaggtgatgt gcttgctacc gataacaata 3061 ctgtaggcat gggtgatgct ggtattaaat ctgccattca aggctctaat gttcctaacc 3121 atgatgaggc tgcccctaat tttgtttctg gtgctatggc taaagctggt aaaggacttc 3181 ttgaaggtgc gttgcaggct ggcacttctg ccgtttctga taagttgctt gatttggttg 3241 gacttggtgg caagtctgcc gctgataaag gaaaggatac tcgtgattat cttgctgctg 3301 catttcctga gcttaatgct tgggagcgtg ctggtgctaa tgcttcctct gctggtatgg 3361 ttgacgccgg atttgagaat caaaaagagc ttactaaaat gcaactggac aatcagaaag 3421 agattgccga gatgcaaaat gagactcaaa aagagattgc tggcattcag tcggcgactt 3481 cacgtcagaa tacgaaagac caggtatatg cacaaaatga gatgcttgct tatcaacaga 3541 aggagtctac tgctcgcgtt gcgtctatta tggaaaacac caatctttcc aagcaacagc 3601 aggtttccga gattatgcgc caaatgctta ctcaagctca aacggctggt cagtatttta 3661 ccaatgacca aatcaaagaa atgactcgca aggttagtgc tgaggttgac ttagttcatc 3721 agcaaacgca gaatcagcgg tatggctctt ctcatattgg cgctactgca aaggatattt 3781 ctaatgtcgt cactgatgct gcttctggtg tggttgatat ttttcatggt attgataaag 3841 ctgttgccga tacttggaac aatttctgga aagacggtaa agctgatggt attggctcta 3901 atttgtctag gaaataaccg tcaggattga caccctccca attgtatgtt ttcatgcctc 3961 taaatcttgg aggctttttt atggttcgtt cttattacct ttctgaatgt cacgctgatt 4021 attttgactt tgagcgtatc gaggctctta aacctgttat tgaggcttgt ggcatttcta 4081 ctctttctca atccccaatg cttggcttcc ataagcagat ggataaccgc atcaagcttt 4141 tggaagagat tctgtctttt cgtatgcagg gcgttgagtt cgataatggt gatatgtatg 4201 ttgacggcca taaggctgct tttgacgttc gtgatgagtt tgtatctgtt actgagaagt 4261 taatggatga attggcacaa tgctacaatg tgctccccca acttgatatt aataacacta 4321 tagaccactg ccctgaaggg gacgaaaaat ggtttttaga gaacgagaag acggttacgc 4381 agttttgccg caagctggct gctgaacgcc ctcttaagga tattcgcgat gagtataatt 4441 accccaaaaa gaaaggtatt aaggatgagt gttcaagatt gctggaggct tccactatga 4501 aatcgcgtag aggctttact attcagcgtt tgatgaatgc aatgcgacag gctcatgctg 4561 atggttggtt tatcgttttt gacactctca cgttggctga cgaccgatta gaggcgtttt 4621 atgataatcc caatgctttg cgtgactatt ttcgtgatat tggtcgtatg gttcttgctg 4681 ccgagggtcg caaggctaat gattcacacg ccgactgcta tcagtatttt tgtgtgcctg 4741 agtatggtac agctaatggc cgtcttcatt tccatgcggt gcactttatg cggacacttc 4801 ctacaggtag cgttgaccct aattttggtc gtcgggtacg caatcgccgc cagttaaata 4861 gcttgcaaaa tacgtggcct tatggttaca gtatgcccat cgcagttcgc ttcacgcagg 4921 acgctttttc acgttctggt tggttgtggc ctgttgatgc taaaggtgag ccgcttaaag 4981 ctaccagtta tatggctgtt ggtttctatg tggctaaata cgttaacaaa aagtcagata 5041 tggaccttgc tgctaaaggt ctaggagcta aagaatggaa caactcacta aaaaccaagc 5101 tgtcgctact tcccaagaag ctgttcagaa tcagaatgag ccgcaacttc gggatgaaaa 5161 tgctcacaat gacaaatctg tccacggagt gcttaatcca acttaccaag ctgggttacg 5221 acgcgacgcc gttcaaccag atattgaagc agaacgcaaa aagagagatg agattgaggc 5281 tgggaaaagt tactgtagcc gacgttttgg cggcgcaacc tgtgacgaca aatctgctca 5341 aatttatgcg cgcttcgata aaaatgattg gcgtatccaa cctgca //