LOCUS MH617306 6009 bp DNA circular ENV 05-AUG-2018 DEFINITION Inoviridae sp. isolate ctcj20, complete genome. ACCESSION MH617306 VERSION MH617306.1 DBLINK BioProject: PRJNA393166 BioSample: SAMN07319269 Sequence Read Archive: SRR5832142 KEYWORDS ENV. SOURCE Inoviridae sp. (animal metagenome) ORGANISM Inoviridae sp. Viruses; ssDNA viruses; Inoviridae; unclassified Inoviridae. REFERENCE 1 (bases 1 to 6009) AUTHORS Tisza,M., Buck,C., Pastrana,D., Welch,N. and Peretti,A. TITLE Uncovering a Universe of Circular DNA Viruses in Animal Metagenomes JOURNAL Unpublished REFERENCE 2 (bases 1 to 6009) AUTHORS Tisza,M., Buck,C., Pastrana,D., Welch,N. and Peretti,A. TITLE Direct Submission JOURNAL Submitted (12-JUL-2018) Lab of Cellular Oncology, National Cancer Institute, 37 Convent Dr., Room 4118, Bethesda, MD 20814, United States of America COMMENT ##Genome-Assembly-Data-START## Assembly Method :: SPAdes v. 3.11.0 Genome Coverage :: 61.0664x Sequencing Technology :: Illumina ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..6009 /organism="Inoviridae sp." /mol_type="genomic DNA" /isolate="ctcj20" /isolation_source="haddock tissue" /db_xref="taxon:2219103" /environmental_sample /country="USA" /collection_date="01-Jun-2017" /note="genome binned from sequencing reads available in SRX3009445; metagenomic; derived from metagenome: animal metagenome" CDS 1..285 /inference="similar to AA sequence:UniProtKB:A0A0B0P4U3_GOSAR" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AXH76073.1" /translation="MIIYMGEILKVENNDGFKTITFEAERYDRGLNKNVPCSEQVIIT DECEHFWSNYQKHIGETVSISVSAVPKKKGGGVWFLTQSDVLDPASVLTA" CDS 505..762 /inference="similar to AA sequence:UniProtKB:A0A0R0CR59_9GAMM" /codon_start=1 /transl_table=11 /product="membrane protein" /protein_id="AXH76074.1" /translation="MSEKYDVVVTQQKKRNKGLYAAATAVAVTALAPVASNAALAEIG AELSTEMEAGKLIIVSIMTLGATLLAMFAGYRYMKRGANSA" CDS 951..2210 /inference="similar to AA sequence:UniProtKB:C8NB77_9GAMM" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AXH76075.1" /translation="MIIKQLTAFFLSFVLIFISTPSFAADPNDFRKPNQWQHERTIND AINKSSVKVEAQKTYPVSTLGPDGKPIKQIRNAKATITLGAKSANVGKSLLKRAPHIA ITQAVTALLLKSVDWVMDAENNRVKYKETDDGVKSWVIDEGGKDETWGSSPESAFRAY TSKVGVDITPKPDKKGLICTTHSNGFSSNCLVEEVASGQVWYKPQNAPPSSDEWKYIP ISDIANKMIQQAEEGQAPAMQVMNDAALDALEAGLLDAQLDAASNVPQPDPQEITQAQ YDNPDYPEAGTGSGTDPETPTDPETPTDPETPTDPDNPFQLPPFCSWASKVCDFIDWM EKDPDEDTQIELPEPDMPDVDSDIAFGGSCPDDAGFTMNIFGNQVEFIMMPFSKFCPL LDTYIKPVLVVLGSFMAVTIVGGRRDG" CDS 2203..2481 /inference="similar to AA sequence:RefSeq:WP_100936082.1" /codon_start=1 /transl_table=11 /product="DUF2523 domain-containing protein" /protein_id="AXH76077.1" /translation="MASGFAGLLKAVNKGWLKDVLSGAGLTLATSGASLFLFNQMLNI FKSNMGSVPVTILSLAHIAGFDIFFSLIFGAYVAKMTLNSGNLVLRGK" CDS 2550..3845 /inference="protein motif:CDD:283385" /note="Zonular occludens toxin (Zot)" /codon_start=1 /transl_table=11 /product="zonular occludens toxin" /protein_id="AXH76078.1" /translation="MLYLATGTPGAKKTVFVVSKLDKVERDNKVNLVKNKEYYAFNKQ LIDRFRSDFGYYESDVGSGHRLKNVIDVLPDDYFDMFAQDFDDLRPDDYFLRTTRFNE ICERINEREGEQKFKFLLPVRTIYTNIKALKIDYVRDIEYDWRDCPDGSIVVIDEVQL VEPFDQTKNKNDPIIQELTIHRHRGFDFYFITQYPSLLHPTVKDLVGVHYHLTVPYGL KTRVYQFGSTRAYPNTMANKFNCETKFYFSPPDRLFKLYKSTTINTHQKRIPYKQLFG FGILILLGVSVFLFSVFGAKDSAIFGGDTKKPAVLAAAAPVVDNPMPTATTGQPDTLT PAPVTGQPDTLTPAPVTASSGIYDPLSGGYINDINLVPVSGMQMNGRCSVYNAYGALL THISNADCNVYLSVKGMMPKVKSSTPKLSAPTAVSPASS" CDS 4315..4566 /note="No HHBLITS hits" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AXH76076.1" /translation="MHYSGVFIMSCINSWSVALSESVPSCSCPPETLPCAFKCDLDEV DGSIFFVSIPVDSDLPFCPFCELVQLDVSAPYACQCEGF" CDS 4723..5994 /inference="protein motif:CDD:177271" /codon_start=1 /transl_table=11 /product="replication initiation protein" /protein_id="AXH76079.1" /translation="MLDMLVIRLPVKPEFIVKHGDLFSVLGDVADYGLASATRFIKRD PVTKEVTHGELYHPYESLPSSYSSMAIKFYDTCRLCPPYMELKASPAKLLQGHNVYGG ESIYNGAAEMFAILESVYPWFFSECLDIEKADVRQIDTTFHACLPSQNLVLPALRFLS SVSNGQRKNDTQKRDFINTVYYGGATSRLVRSKIYAKHNEVKNTIEKLQKKSNRGCVA SFDALKIYTPELIEYSKGLLRFEHSVMARKLERSGLPTNLWRLIEYQNSNPDLLQQLW LDGFKPILKALEGEVMEHADDKEIQNLCMKKLVTITPSGKESFTKANNAFNFYQLLKM NGWLDVKSRYTVSQFNRNVKALVDIGVPKALLQNLGKERGKVILMIDLLKIDFFNQAP EGYKPPNTKHYPDYDHYFSKSVKRKKLRLVA" ORIGIN 1 atgattattt atatgggtga gattttaaaa gttgagaata acgatggttt caaaacgatt 61 acgtttgaag ctgagcgtta tgaccgtggc ttgaataaaa atgttccatg ttctgagcaa 121 gtgattatca ctgatgaatg tgagcatttt tggtcgaatt atcaaaaaca tattggtgaa 181 acagtttcta tttctgtttc tgctgtaccg aaaaagaaag gcggtggcgt ttggttttta 241 acacagtctg atgttttaga tcctgcttcc gttctcactg cttaataagg atttcccatg 301 tacgtttgca atgctctcga tgaggcgtcc aatacgtgct tggaatgggt cgcctacaat 361 gattattctt tgctttctga gttagcaata accaaagctg aaatggtcac gataggcggt 421 caagttgggg ctattttatc cattatttta gcctttgtta tggtgtttaa agcacttaaa 481 ttaatgtaat cactggagtt tgttatgtct gaaaaatacg atgttgtagt tactcaacaa 541 aaaaaacgta acaaaggttt atatgctgct gctactgctg tagctgttac cgcccttgcc 601 cctgttgcat ctaacgctgc tctagctgaa attggtgcag aattaagcac tgaaatggag 661 gcaggtaagt taatcattgt ctcaattatg acgcttggtg ctaccttgct agctatgttt 721 gcaggttatc gttacatgaa acgtggcgcg aacagtgcct aattttttat caggttattg 781 gggtcatggg cttttgctcg tggctctaat aattaagggg tttatatgtt actggctgtt 841 gtcgatcctg atgcctctat tttctggtgg ttgcctattc tgttgatatt ttttgctttt 901 atcgttctta ttcgcacttt ccgctagaaa ataataaaaa ggtttttaat atgattatca 961 aacaattaac agcctttttc ttatcttttg tccttatctt tataagtaca ccatctttcg 1021 ctgctgatcc aaacgacttc cgcaagccta accaatggca gcatgagcgt actattaacg 1081 atgctatcaa taaatcatct gttaaagttg aagcgcaaaa aacataccct gtttctactc 1141 ttggtcctga tggcaaacct attaaacaaa ttcgcaatgc aaaagccaca ataacattgg 1201 gcgctaagtc tgctaatgtc ggtaagtctt tacttaaacg tgcccctcat attgccatca 1261 ctcaagctgt caccgcttta cttttaaagt ctgttgattg ggttatggac gctgaaaata 1321 atcgcgttaa atataaagaa actgatgatg gtgttaaaag ttgggttatt gatgagggtg 1381 gtaaagatga aacttggggt tctagtcctg agtcagcttt tagggcttat acttctaaag 1441 ttggagttga tattactcca aagcctgata aaaaaggttt aatttgtact actcattcaa 1501 atggtttttc ttcaaattgt cttgtggaag aagtagcttc tggtcaagtt tggtataaac 1561 ctcaaaacgc tcctccatct tctgatgaat ggaagtatat ccctatttcc gatattgcta 1621 acaagatgat tcagcaagct gaagaaggtc aagcgcctgc aatgcaagtt atgaatgatg 1681 cagcccttga cgctctcgaa gctggtttac ttgatgctca attagacgca gcgtctaatg 1741 ttccacagcc tgacccacaa gaaattacac aagctcaata tgataaccct gattatcccg 1801 aagcaggtac tggctcgggt actgatcctg aaacacctac agaccccgaa acacctacag 1861 accccgaaac acctacagac cccgataatc cttttcaatt accgccgttt tgctcttggg 1921 cttctaaggt ttgtgatttt atagattgga tggaaaaaga ccctgacgaa gatacgcaaa 1981 tagagttgcc tgagcctgat atgcctgatg ttgattctga tatagctttt ggtggttctt 2041 gtcctgatga tgcagggttt accatgaaca tatttggtaa tcaggtcgaa tttatcatga 2101 tgccctttag taagttttgt cctttgcttg atacctatat aaagcctgtt ctcgttgttc 2161 ttggctcttt catggctgta actattgtag gaggtcgtcg cgatggctag cggttttgca 2221 ggtctattaa aagcagtaaa taaaggttgg cttaaagatg tgttatcagg tgcaggtctt 2281 acccttgcca ccagtggcgc gtctttgttt ttgtttaatc agatgcttaa tatctttaaa 2341 tcaaatatgg gctctgttcc tgtcactatc ttgtcactgg ctcatattgc aggttttgac 2401 atatttttta gtcttatctt tggcgcatac gttgcaaaaa tgaccctaaa ctcaggaaac 2461 ctagtgttaa ggggaaaata aagctgcgct acgtgtgaga aggaggcgac gacgacgagc 2521 actaagcagc gttaaattaa ggattattta tgttatatct tgctactggc acaccaggtg 2581 ctaaaaaaac tgtctttgtt gtttcaaaat tggataaggt cgagcgagat aacaaagtta 2641 atcttgttaa gaataaggaa tattatgcgt tcaataaaca gcttattgat agattccgtt 2701 ctgatttcgg ttattacgaa tctgatgtcg gttcaggtca tcgtctaaaa aacgtcattg 2761 atgttttgcc tgatgattac tttgatatgt tcgcccaaga ttttgatgat ttacgccctg 2821 atgattattt tttacgtacc actcgtttta atgagatatg tgagcgtatc aatgagcgtg 2881 agggagagca gaaatttaag tttctgctac ctgtccgtac catatacaca aacattaaag 2941 cccttaaaat agattacgtt cgtgatatag aatatgattg gcgtgattgt cctgacggct 3001 ctatcgttgt tattgatgaa gttcagcttg tcgaaccatt tgaccaaaca aagaataaaa 3061 atgaccccat tattcaagaa ttaactatcc atcgtcatag gggttttgat ttttacttta 3121 tcactcagta ccctagctta cttcacccca ctgtcaagga tttggttggc gttcactatc 3181 atttgactgt gccctatggt ttgaaaactc gtgtttatca gttcggttct actcgtgctt 3241 atccaaacac aatggccaat aagtttaact gtgaaactaa gttttatttt tccccacctg 3301 atcgtctttt caagctttat aaatctacta ccattaatac ccatcagaaa cgcataccgt 3361 ataagcagtt attcggtttt ggtatcctga ttttattagg tgtgtctgtt ttcttattct 3421 ctgtctttgg tgctaaggat tccgctattt ttggtggcga cactaagaaa cctgctgttc 3481 ttgctgctgc tgcgcctgtc gttgataatc ccatgcccac tgctactact ggacaacctg 3541 acacgctcac ccctgcgcct gttactggtc aacctgatac tcttacgcct gcgcctgtta 3601 ctgcatctag tggcatttat gaccctttaa gcggtggtta tatcaatgat attaacctcg 3661 tgcctgtgtc gggtatgcaa atgaatgggc gttgttctgt ttataatgct tatggtgctt 3721 tgcttactca tatctctaat gccgattgca atgtttattt atctgtcaaa ggtatgatgc 3781 caaaagttaa aagttctaca cctaaactgt ccgcacctac tgcggtaagt cctgcatcaa 3841 gttaaacttc tttttctcca gttaacatct ttttggtaat tggtgtctca gttttctggg 3901 tttggcgggt agtaattgat aacatctttt cgttatcaat tacgttaccg cattgcatga 3961 ccctgattgg tgccgtgaga accaagagag tccacactat ttagttgtgg actctttgcg 4021 catttttgcg ttattttaat ttttgcctct gcttattatc tctaatattt cctcatttgt 4081 tatttctttt agtccctttt ctgctgctgc ttctatcatt tgtgcttctg ttattagttc 4141 tcttttttct gccacgaaat ccgctgccct ttcttttgca tcttctgata gtctttcgtc 4201 cattctgtac gtttttgttc ccatttcttt ccccttgtta caattaaatt aagtttcttt 4261 gttgacttgt atctaaaaaa cattgtaaca tcgtttcaat gtttagaagt tttcatgcac 4321 tattctggag tgtttattat gtcatgtatt aattcttggt cggtggctct ttccgaatct 4381 gtaccctctt gctcctgtcc tccagaaact ttgccatgcg ctttcaaatg tgatcttgat 4441 gaagtggacg ggagcatctt ttttgtttct attcctgttg attctgattt gcctttttgt 4501 cctttttgtg agttggttca acttgatgtt tctgctcctt atgcttgtca atgtgagggt 4561 ttttgatatg aattatcagc cttttattgg tatgcatgtt ttgctatcta atgagtttat 4621 tgccttcttt catttttgcg gttttgttgc tcttggcgtt ttgcttttgc ttttgcttat 4681 tgcttatcaa aaacaacgtg ccactgatgt gaggtctggt tcatgttaga tatgttagtt 4741 atacgattgc cagttaagcc agaatttatt gtcaagcatg gtgatttatt ctctgttctt 4801 ggtgatgttg ctgattatgg tctggcttct gcgactcgtt ttattaaacg tgatccagtg 4861 actaaggaag tcacccacgg tgagttgtat cacccttacg agtctttgcc ttcttcctat 4921 agcagtatgg cgattaagtt ttatgatacg tgccgtttat gccctcctta tatggaattg 4981 aaggcaagcc ctgctaagtt attgcaaggt cataacgtct atggcggtga gtctatttat 5041 aacggtgctg ccgaaatgtt tgcaatttta gagtctgttt atccttggtt cttctctgaa 5101 tgtttggata ttgaaaaagc agatgtgcgc cagatagaca caactttcca tgcttgtttg 5161 ccttcacaga atttagtatt acctgcctta cgtttcttat cttctgtttc taatggtcaa 5221 agaaaaaacg atacacaaaa aagagatttt attaatactg tttattatgg tggtgctaca 5281 tcacgtttgg tgcgctcgaa gatatacgca aagcataatg aagtcaaaaa tactatagaa 5341 aaattacaga aaaaatctaa ccgtggttgt gtcgcatcat ttgacgcttt aaaaatttat 5401 acccctgagt taatagagta ttcaaaaggc ttgctgcgct ttgaacactc cgttatggct 5461 cgtaagctcg aacgctctgg attgcccaca aatttgtggc gtttgattga gtaccaaaat 5521 agtaaccctg acctattaca acaattatgg cttgacggtt ttaaacctat tcttaaagcc 5581 ttggaaggtg aagttatgga acatgctgat gataaagaga ttcaaaatct ctgtatgaaa 5641 aagttagtca cgatcactcc tagtggtaaa gagagtttta ccaaagccaa caatgctttt 5701 aacttttacc agttgttaaa aatgaacggc tggttagatg ttaaatctcg ttataccgtt 5761 tctcaattta atagaaatgt taaagctctt gttgatattg gtgtgcctaa agccctctta 5821 caaaatctag gtaaagaacg tggcaaggtt attttgatga ttgatttatt aaagattgat 5881 ttctttaatc aagcaccaga aggctataaa ccgcctaaca caaagcatta tccagactat 5941 gaccattatt ttagtaaatc agttaaacgc aaaaagctac gtctagtagc ttaaatagga 6001 gcattaaag //