LOCUS MN582079 4509 bp DNA circular ENV 18-NOV-2019 DEFINITION Microviridae sp. ct0DW36, complete genome. ACCESSION MN582079 VERSION MN582079.1 DBLINK BioProject: PRJNA540674 BioSample: SAMN11552893, SAMN11552892, SAMN11552894 Sequence Read Archive: SRR8996939, SRR8996941, SRR8996944 KEYWORDS ENV. SOURCE Microviridae sp. ct0DW36 (freshwater metagenome) ORGANISM Microviridae sp. ct0DW36 Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae. REFERENCE 1 (bases 1 to 4509) AUTHORS Malki,K. and Breitbart,M. TITLE Florida's Freshwater Springs JOURNAL Unpublished REFERENCE 2 (bases 1 to 4509) AUTHORS Malki,K. and Breitbart,M. TITLE Direct Submission JOURNAL Submitted (16-OCT-2019) College of Marine Science, University of South Florida, 830 1st St S, Saint Petersburg, FL 33701, USA COMMENT Submissions were annotated with Cenote-Taker2 available at https://github.com/mtisza1/Cenote-Taker2. ##Genome-Assembly-Data-START## Assembly Method :: SPAdes v. 3.12 Genome Coverage :: 23.492570x Sequencing Technology :: Illumina ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..4509 /organism="Microviridae sp. ct0DW36" /mol_type="genomic DNA" /isolate="ct0DW36" /isolation_source="Volusia Spring" /db_xref="taxon:2656693" /environmental_sample /country="USA" /collection_date="22-May-2017" /metagenome_source="freshwater metagenome" /note="genome binned from sequencing reads available in SRX5775735,SRX5775734,SRX5775731; metagenomic" CDS 1..921 /inference="protein motif:PFAM:PF05840.12" /codon_start=1 /transl_table=11 /product="replication associated protein" /protein_id="QGH72835.1" /translation="MACYYPITGYKSRQLNSTGKRSIVFNSKFGFDDMPVVINCGRCV GCRLEYSRQWAMRCMHESQLYDDNCFLTLTYDDAHLPADCSLNMRDMQLFWKRLRKFY GKKLRYYMAGEYGSVTYRPHYHACVFGFRPDDLEIYTVRDDITLYTSAVLEDLWGHGF VTVGELNFETAAYTARYVMKKFKSSDEQAKIDHYRHVNYETGEFVDVVPEFVAMSRRP GIGRDWLSKFSADVYNHDFCVINGQKVRPPKFYDSVYELSDPDHFHLIQQARVTAGRD DSVQVNRTPERLDARRKVHEYRAKLLARTL" CDS 941..1243 /inference="similar to AA sequence:UniProtKB:A0A0G2UEC9_9VIRU" /note="DNA binding protein VP5" /codon_start=1 /transl_table=11 /product="DNA binding protein" /protein_id="QGH72836.1" /translation="MIYHIFSVFDYGAEHYMTPFYVENEKLAVRMFSDICQDSDSAIA RHPSDFSLFCLGSFNNCSGDFNIHPQPELVSKAVSFVDSRSAADHIARAVVAASDS" CDS 1265..2866 /inference="protein motif:PFAM:PHA00363" /codon_start=1 /transl_table=11 /product="major capsid protein" /protein_id="QGH72837.1" /translation="MKSVMAHNFSQVPRAEIQRSHFNRSHRHSTTFDAGYLVPVFVDE VLPGDTFNCDMTGFARLATPIHPLMDNLFMDTFYFAVPYRLVWDNWQKFNGEQTDPGD STDYVIPTMTSPVGGYLEGSLSDYFGIPTKVAGLEHSSLFHRAYNLIWNEWFRDQNLQ DSVVVDTDDGPDSPTDYALLRRGKRHDYFTSALPWPQKGPAVDLPLGTSAPVIGTSQS ITLTNGTTSAAMANFDGTSDYLASAVGQAGGAVGATLTGSAFTGSKLLGLATSDSGIV ADLSEATAATINELRQAFQIQKLYERDARGGTRYTEIIRAHFGVVSPDARLQRPEYLG GGSTPVNIHPIAQTSSTDVTTPQGNLAAFGTAMMHNHGFTKSFTEHCLVIGLISVRAD LNYQQGLNRMWSRQTRWDFYWPALSHIGEQAVLNKEIFAQGTSADEDVFGYQERYAEY RYKPSVVTGLFRSNATGTLDSWHLAQDFDTLPTLSDEFIVEDPPVDRVIAVPSQPHFL FDSYFNLNCARPMPVYGVPGLIDHF" CDS complement(2863..3168) /note="No HHsearch hits" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QGH72841.1" /translation="MRPHGLHSRGHGRSNGILVLVTSTRRSAGTTASSGQNRIESGRL EIGNVTLPCSRRHSLLKSHLGISDVRIVLISRLLTEKTSTSRQCRQSKRNPGHKITS" CDS 2980..3570 /inference="similar to AA sequence:UniProtKB:A0A0C5AFV1_9VIRU" /codon_start=1 /transl_table=11 /product="putative minor capsid protein" /protein_id="QGH72838.1" /translation="MAFQERMSSTARQRDVADLKAAGLNPILAAGGGGSSTPSGASYQ YQDPIGPSMSSAMQAMRTHAEVANMRLQNQSIAAEIARTNADTRVRDAQASNLAVQNA NILADTDVKKTQRVLNELSRANVRADAYLKSSSARSTQANAKLLEAQVPGAVTESKID QSKYGKTIRWLNRGTDAAGKLFHSAVNAARFKFFME" CDS 3567..4043 /inference="protein motif:PFAM:PF09675.9" /codon_start=1 /transl_table=11 /product="scaffold protein" /protein_id="QGH72839.1" /translation="MRFFYMSKNAVVSRFRSAYDASVRVSKTFNSPTRTHQSFKNECD INRILDRWQKTGVITHYNKYSANYSDLTSVPDYRQAMDQIIRANQAFESLPSSLRKRF GNDPAEFLSFVSSPDNVDEMVKLGLANKRDEVVTPQPFVPAPGQIPIVAPVSAPAA" CDS complement(4327..4488) /note="No HHsearch hits" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QGH72840.1" /translation="MTYDKKTTKIITGWGPNYSTLQTYTSPHGNTANISSMNTTSSCS KQPPRASTS" ORIGIN 1 atggcgtgtt attatcccat tactggttat aaatcgagac agttaaattc aactggtaaa 61 cgctcgatag ttttcaattc caaatttgga tttgatgata tgccggtagt tattaattgt 121 ggtcggtgcg ttggttgtcg tttggagtat tccaggcaat gggctatgcg ctgtatgcac 181 gaatcgcaat tgtatgatga taattgtttt cttaccttaa catacgatga tgcccatctc 241 cctgcagatt gttctctcaa tatgagggac atgcagttat tttggaaacg gttgaggaaa 301 ttttatggaa agaaattgcg atactacatg gctggggaat atggctctgt tacttatagg 361 cctcattacc atgcctgcgt ttttgggttt aggcctgatg atttggaaat ctacacggtt 421 cgagatgata ttacattata tacatcagct gttttagagg acctctgggg ccatggtttt 481 gttaccgtcg gagagctgaa ttttgagacc gctgcttaca ctgcgcggta tgttatgaaa 541 aaattcaaat cgtctgatga acaggccaaa attgatcatt atcgtcatgt taattatgag 601 actggtgaat ttgttgatgt tgttcctgag tttgttgcta tgtctcgtcg tcctgggata 661 ggtcgagact ggttatctaa attttcggcc gatgtttaca atcatgattt ttgtgtgatt 721 aatggtcaaa aagttcgtcc cccaaaattt tatgattctg tttatgagtt gagtgatcct 781 gatcattttc atttgatcca acaggctcgt gtgactgctg gtcgcgatga ttcggttcag 841 gttaatagga ctcctgaacg tttagatgct cggcgtaagg tccatgaata tagggctaaa 901 ttgttggccc gtacattata gttagagatt ggagtatttt atgatttatc atatttttag 961 tgtatttgat tatggtgcag agcattatat gaccccgttt tatgttgaga atgagaagct 1021 agctgttcgc atgttttctg atatatgtca ggattctgat tctgctattg ctcgtcatcc 1081 tagtgatttt tcgttgtttt gtttaggttc gtttaacaat tgttctggtg attttaacat 1141 tcacccccag cctgaattgg tttcaaaggc ggtttcgttt gttgattctc gatctgctgc 1201 tgatcatatt gctcgcgctg ttgttgctgc gtctgattca taattttatt tttaggagtt 1261 ttctatgaaa tcggttatgg ctcataattt tagtcaggtt cctagagctg agattcaacg 1321 ttctcatttt aaccgttcgc atcgtcattc tactacgttt gatgccggtt atctggttcc 1381 tgtttttgtt gacgaagttc tccccggtga tacatttaat tgtgatatga ccggttttgc 1441 ccgtctcgcc actcctattc atccactgat ggataatttg tttatggata cattttattt 1501 tgctgtcccc tatcgtttgg tttgggataa ctggcagaag tttaacggtg aacaaactga 1561 tccgggtgac tctacggatt atgttattcc tacgatgacc tcacctgtgg gtggttattt 1621 agaaggttct ctctcagatt attttggtat tcccactaag gttgccggtt tggaacattc 1681 tagtttgttt catcgtgctt ataatttgat ctggaatgaa tggtttcgtg atcaaaattt 1741 acaggattcc gttgttgttg atactgatga tggtccagat tctcctactg attatgcgtt 1801 gttacgtcga ggcaaacgtc atgattattt tacatctgcg ttgccatggc ctcaaaaagg 1861 ccctgccgtt gatcttccgt tgggtacatc tgcccctgta attggtacgt ctcagtctat 1921 tacgttgacg aatgggacga cttcggctgc aatggccaat tttgatggta cgtcagatta 1981 tcttgcttct gctgttggtc aggctggtgg cgctgttggt gccacgctaa ctggttctgc 2041 atttactggt tcaaaattgc ttggtttagc tacgtctgat tctggtattg ttgctgattt 2101 gtcggaggct actgcggcga ctattaatga attgcgtcag gcgtttcaga ttcagaaact 2161 gtatgaacgt gatgctcgcg gtggtactcg ttacactgag attattcgtg cacattttgg 2221 tgttgtttct cctgatgctc ggttacaacg tcccgaatat ctaggtggtg gatcaacccc 2281 tgttaatatt catccgattg cacaaaccag ttctactgat gtgactacac ctcaaggtaa 2341 tttagctgcg tttggtactg ccatgatgca taatcatggg tttacaaaat cgtttactga 2401 gcactgcctg gtcattggtc ttatttctgt tcgtgctgac ctgaattatc aacagggact 2461 taatcgtatg tggtctcgtc aaacccgttg ggatttttat tggcccgcat tgtctcatat 2521 tggcgaacag gcagttttga acaaagagat tttcgctcaa ggtacgagtg ccgatgagga 2581 tgtttttggt tatcaagaac gttatgctga gtaccgttac aaaccgtctg tcgttactgg 2641 tttgtttcgt tctaatgcga cgggtacgct cgattcctgg catttggctc aggattttga 2701 cacgcttcct actttgtctg atgaatttat tgttgaggat ccacccgtgg atcgcgttat 2761 tgctgttccg tcacagcctc actttttgtt tgattcgtat tttaatttaa attgtgctag 2821 acctatgcct gtttatggtg ttcctggtct gattgatcat ttctaggagg tgattttgtg 2881 gcctggattc cgtttgcttt gtctgcattg tctgctggtg ctagtttttt cggtcagcag 2941 tctgctaatc aaaacaattc tgacatcgct aatgcccaga tggcttttca ggagagaatg 3001 tcttctactg caaggcaacg tgacgttgcc gatctcaagg cggccggact caatccgatt 3061 ttggccgctg gaggcggtgg ttccagcact ccgtctggtg ctagttacca gtaccaggat 3121 cccattggac cgtccatgtc ctctgctatg caggccatgc ggacgcatgc cgaggttgct 3181 aatatgaggt tgcagaatca atcgattgct gctgaaattg cacgaactaa tgcggatact 3241 cgtgtacgcg atgctcaggc cagtaatttg gctgtccaaa atgctaatat tttggctgac 3301 accgacgtta aaaaaacaca acgtgttttg aatgaattat cgcgtgctaa tgttcgtgct 3361 gatgcctatc ttaagtccag ttctgcgagg tcgacgcagg ctaatgctaa gttgttggag 3421 gctcaggtac ctggtgccgt gactgaatca aaaattgatc agtctaaata tggtaaaacc 3481 attcgttggc taaatcgtgg tactgatgct gctggtaagt tgttccattc agctgttaat 3541 gctgctcgtt ttaaattttt catggaatga ggttttttta tatgtctaaa aatgctgttg 3601 tctctcgttt tcgttctgca tatgatgcta gcgtacgtgt ttctaaaacg ttcaatagtc 3661 ctactcgtac gcatcaatcg tttaaaaatg aatgtgatat taatcgcatt ttggatcgtt 3721 ggcagaagac tggtgtaata actcactata ataaatattc tgctaactac tctgatttaa 3781 cgtcggttcc tgattatcgt caagctatgg atcagatcat tagagctaat caggcgtttg 3841 agtctctccc tagttcgtta cgtaaacggt ttggtaatga tcctgcggag tttctatcat 3901 ttgtctcgtc tcctgataat gttgatgaaa tggttaaact tggtttagct aataaacgtg 3961 atgaggttgt tactcctcag ccgtttgttc ctgctcctgg gcagattcct attgttgctc 4021 ctgtttctgc ccctgcagca taattttgag gtccgaccaa ttctcttctt gatgtaattg 4081 gtcggactga caccgctagg tgtctgtcta tgtgtttcta acacattttt cacggagtga 4141 aattggtttt tttgttaccg tttttttaga cggctgtcaa ggctccgcag gacccgcagg 4201 gcttggcctt gacagccgtc taaaaaaatg ttattagttt agtatcccca atgataagcc 4261 cccgcaaatt gtggggcttt ttctaattta gttaaatagg gaggtgattt tatgcgtaga 4321 tctaaattaa ctcgtcgagg ctctcggagg ttgtttactg caactgctcg tcgtgttcat 4381 cgaactaatg ttcgcggtgt tcccatgagg ggaggtatac gtttgtaggg tggagtagtt 4441 aggaccccac cctgttatta ttttagttgt ttttttgtca tatgtcatct gagttaggcg 4501 gaggaattt //