LOCUS MN582105 4434 bp DNA circular ENV 18-NOV-2019 DEFINITION Microviridae sp. ctOsc38, complete genome. ACCESSION MN582105 VERSION MN582105.1 DBLINK BioProject: PRJNA540674 BioSample: SAMN11552893, SAMN11552892, SAMN11552894 Sequence Read Archive: SRR8996939, SRR8996941, SRR8996944 KEYWORDS ENV. SOURCE Microviridae sp. ctOsc38 (freshwater metagenome) ORGANISM Microviridae sp. ctOsc38 Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae. REFERENCE 1 (bases 1 to 4434) AUTHORS Malki,K. and Breitbart,M. TITLE Florida's Freshwater Springs JOURNAL Unpublished REFERENCE 2 (bases 1 to 4434) AUTHORS Malki,K. and Breitbart,M. TITLE Direct Submission JOURNAL Submitted (16-OCT-2019) College of Marine Science, University of South Florida, 830 1st St S, Saint Petersburg, FL 33701, USA COMMENT Submissions were annotated with Cenote-Taker2 available at https://github.com/mtisza1/Cenote-Taker2. ##Genome-Assembly-Data-START## Assembly Method :: SPAdes v. 3.12 Genome Coverage :: 17.477793x Sequencing Technology :: Illumina ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..4434 /organism="Microviridae sp. ctOsc38" /mol_type="genomic DNA" /isolate="ctOsc38" /isolation_source="Volusia Spring" /db_xref="taxon:2656698" /environmental_sample /country="USA" /collection_date="22-May-2017" /metagenome_source="freshwater metagenome" /note="genome binned from sequencing reads available in SRX5775735,SRX5775734,SRX5775731; metagenomic" CDS 1..1038 /inference="protein motif:PFAM:PF05840.12" /codon_start=1 /transl_table=11 /product="replication associated protein" /protein_id="QGH73504.1" /translation="MACHRPLYGYVSRTVNPSGKRSTVWNAREGYYDLPRTLRCGKCD GCCLDWSASWAVRCIHEASLHENNIFLTLTYADEFLPEGGTLVKADWQKFMKRLRKRY GAGARYFHCGEYGKKKGRPHYHALLFNFEFDDQVKWRKTRGGYQTFRSPSLEELWPFG HSEIGSVTFDSAAYVARYILKKVRGPEAEAHYEGRIPEYLTMSRRPGIGKDWIRKHSA DVYGRDVVVIPREGGARMLRPPRYYDAHYEVENPEGYANIRAARRLKAREAAEKAGAE RLGALEKIAALNVSRQQRGYESEAQDVFGFRWEDRGVHDPLLHAACGAGDPVFRADGE RSGDGSVEVPG" CDS 995..1177 /inference="protein motif:CDD:222780" /codon_start=1 /transl_table=11 /product="nonstructural protein" /protein_id="QGH73503.1" /translation="MVNDPETVPSKYPADFRLVELGEWDTTTGIYSENEVPKALGSAL DYKRRPQDELPLKAVQ" CDS 1179..2843 /inference="protein motif:PFAM:PHA00363" /codon_start=1 /transl_table=11 /product="major capsid protein" /protein_id="QGH73505.1" /translation="MLGRGSMGRLPSPNSHNFASVPRAEIPRSQFNRSHNFKTTLDAG YLVPIFVDEALPGDTFRLRMSTFARMATPIVPVMDNLYADFFFFAVPYRLVWNNWQKF NGEQTDPGDSTSYLVPVMTAPAVTGHAIGSLSDYFGIPTGVPLLEHNSLHHRAYNLVY NEWFRDQNLQDSVVVDKDDGPDASTDYVLLRRGKRHDYFTSCLPWPQKGTAVSIPLGT SAPVYGPTLTDGTGLALFQHWNNTDGAVTFGSLEKTAGALTVSQANLWAGDVAGDSVD SYNLGNAAQYTSAGANYTPPFADLSNATAATINSLRQAFQIQKLYERDARGGTRYIEI IRSHFGVVSPDARLQRPEFLGGGSSRITVTPIPQTSSTDATTPQGNLAAMGTSSGSGI GFVKSFTEHCLLIGMVSIRADLNYQQGLNRMWSRQTRWDFYWPALAHIGEQAVLNKEI FADASAADDEVFGYQERFAEYRYKPSVITGQFRSTAATPLDTWHLAQDFAALPTLDAD FIVEDPPVDRVIATPTEPQFLFDAYFDLVCARPMPVFSVPGLIDHF" CDS 2843..3694 /inference="similar to AA sequence:UniProtKB:A0A0C5AFV1_9VIRU" /codon_start=1 /transl_table=11 /product="putative minor capsid protein" /protein_id="QGH73506.1" /translation="MAEFMDNGGGGGGGGGGGFGIPDPFGPISAFVDAGATMGAAALQ YQGVQATNAANIQMAADANRLEKQLQLRGQKFNAAQALMQRQFEERLSNTAYQRGTAD MRAAGINPMVAFMKGGASTPGGASASSPGASAHKATVENAMGAAVGTAVQVRGLLKDL EAKTAGISLDRAATKAKEAEAEYNSNSAALAATNLKKAQAELPAVAAESKLRKKTADI DEKWAEGDAYLNRLGGVAGTLGTTAKMLREAIGKDRKEKEGRERRRTFDDWLKGTNRM PNAIPGD" CDS 3694..4197 /inference="protein motif:PFAM:PF09675.9" /codon_start=1 /transl_table=11 /product="scaffold protein" /protein_id="QGH73507.1" /translation="MEFRTRFTKKERSGKVCAGGRTLQASRDECNVNLIMARFEKTGQ LPQMIRRDPQYGEFASAPEYHEAMCLVVKAQEQFEALPAQVRKRFGNDPAAFLGFAVD PQNQRELVRMGLATLNEDHAKMESAKLAAESRKDSEQEEEPPPKKAPVRSEATRRGRA AGGGPSA" CDS 4284..4403 /note="No HHsearch hits" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QGH73508.1" /translation="MRGRRRMSKRRSRRLFTRTAKRVHRRNGGGRVMRGGVRL" ORIGIN 1 gtggcctgtc atcgccccct atatgggtac gtgtcgcgta ccgtaaatcc cagcgggaag 61 cgtagcaccg tctggaacgc tcgtgaaggg tattacgatc ttccgcgcac cctgaggtgc 121 gggaagtgcg atggctgttg cctcgattgg tccgcctcgt gggcggtccg gtgtatccac 181 gaggcatccc ttcacgagaa taatattttt ctgaccttga cttacgccga cgagttccta 241 cccgaagggg ggacactcgt taaggcggat tggcaaaagt tcatgaagcg gctgcgtaag 301 cggtatggtg ctggtgctag gtactttcat tgcggcgagt acgggaaaaa aaaaggccgc 361 ccgcattatc acgccttact gttcaatttc gagttcgacg accaagtaaa atggcgcaaa 421 actaggggcg gctatcaaac gtttcgctcg ccgtctctgg aagagctgtg gccgttcggc 481 cactcggaga taggttcggt aaccttcgat tctgccgctt acgtagctcg ctatatccta 541 aagaaagtgc gcgggccgga ggccgaggcg cactacgagg gacggatccc ggaatacctc 601 acgatgtccc ggcgtccggg gatcgggaag gactggatcc ggaagcactc agcggacgtg 661 tacggccgcg atgtggtggt gatcccgcga gagggcggag cgaggatgct tcgcccgccg 721 cgttattatg acgcacatta tgaggttgaa aatccggagg gatacgcgaa catcagggct 781 gcacggaggc tcaaggcccg agaggcagcg gagaaggccg gtgccgaaag actcggggcc 841 ttggaaaaaa tagcggcact gaatgtgtcg cggcaacaac gaggatacga gagtgaagct 901 caagatgttt tcggttttcg atgggaagat cggggcgtac atgaccccct tcttcatgca 961 gcatgcgggg caggcgatcc ggtctttcga gcagatggtg aacgatccgg agacggttcc 1021 gtcgaagtac ccggctgact tccgcctggt cgagctgggc gagtgggata cgaccacggg 1081 gatttactcg gagaacgagg tccccaaggc gctggggagt gcgctggact acaagcgccg 1141 tcctcaggac gagcttcccc tgaaggcggt gcagtaggat gctgggccgg ggttcgatgg 1201 gcaggcttcc gtcgccgaac agtcacaact tcgcctcggt acctcgcgca gagatacccc 1261 ggtcccagtt caaccgaagt cacaatttca agacgacgct cgacgccgga tacctcgttc 1321 cgattttcgt ggacgaggcg ctccccggcg acacgttccg gctacggatg agcacgttcg 1381 cccggatggc gacgccgatc gtgccggtga tggacaacct gtacgcggat ttcttcttct 1441 tcgccgttcc gtatcggctg gtgtggaaca actggcagaa gttcaacggc gagcagacgg 1501 atccgggaga ttcgacgtcg tacctggtgc ccgtgatgac ggctccggcc gtgacggggc 1561 acgcgatcgg atcgctctcg gattacttcg ggatccccac gggggtcccg cttttggagc 1621 acaattcgct tcaccaccgg gcttataacc tggtctacaa cgagtggttc agggatcaga 1681 acctccagga ttcggtggtg gtggacaagg acgatggtcc ggacgcgtcc acggactacg 1741 ttcttcttcg tcgtggcaag cgccacgact acttcacgtc gtgcctgccg tggcctcaga 1801 aaggcacggc ggtgagcatt ccgcttggta cgtcggcgcc ggtgtacggg ccgactctca 1861 ccgacgggac gggcctggcg ctgttccagc attggaacaa cacggatggg gccgtcacgt 1921 tcgggtcttt ggagaagacg gcgggggctc ttacggtgag ccaggcgaac ctgtgggccg 1981 gtgacgtggc tggcgactcg gtcgacagct acaacctggg gaacgccgca cagtacacgt 2041 cggcgggcgc gaattatacg cctccgttcg cggatctttc gaacgcgacc gccgcgacga 2101 ttaatagtct tcgtcaggcg ttccagattc agaagctcta cgagcgggac gcgcgaggcg 2161 ggacgcggta catcgagatc atcaggtctc atttcggagt ggtgagcccc gacgcccggc 2221 tacagaggcc ggagtttttg ggcggcggga gctcgcggat cacggtgact ccgattcctc 2281 agacgagctc gacggacgca accacgccgc aggggaacct ggcggcgatg gggacttcgt 2341 ccgggagcgg gatcggcttc gtgaagagtt tcacggagca ctgtcttttg atcgggatgg 2401 tgtcgattcg ggccgatctg aactaccagc aggggctcaa caggatgtgg agccgtcaga 2461 cgcgttggga tttctactgg cccgctctcg cgcacattgg cgagcaggcc gtattgaaca 2521 aggagatttt cgcggacgcg tcggcggctg atgacgaggt tttcggctat caggagcggt 2581 tcgcagagta ccgctacaag ccgtcggtca tcacggggca gtttcggtcg acggcggcga 2641 cgccgctcga tacctggcat ttggcgcagg acttcgcggc gctgccgacg ctcgacgcgg 2701 atttcatcgt ggaggatccg ccggtcgatc gcgtgattgc gacgccgacg gagcctcagt 2761 tccttttcga cgcgtatttc gatttggtct gtgcacggcc aatgccggtg ttctccgtcc 2821 cgggtctcat cgatcacttc tgatggcaga gttcatggac aacggagggg gtggaggtgg 2881 cggagggggc gggggttttg gtatccccga ccccttcggg cccatctcgg cttttgtaga 2941 cgccggcgcc acgatgggcg ccgcggctct gcagtatcag ggtgtacagg ctacgaacgc 3001 ggccaatatt cagatggccg ccgacgcgaa tcggctggag aagcagctcc agcttcgcgg 3061 tcagaagttc aatgccgctc aggcgctcat gcagcgccag ttcgaggagc gactttcgaa 3121 cacggcctac caacggggga cggccgacat gagggcggcc gggatcaacc cgatggtagc 3181 gttcatgaag ggcggcgcgt ccactcctgg tggagcgtcc gcttcgagtc cgggggcttc 3241 cgcgcacaag gctaccgtgg aaaacgcgat gggtgcggcc gtaggaacgg ccgtccaggt 3301 gcgcggcctc ctcaaggatc tggaggcgaa gacggcgggc atttctttgg atcgtgcggc 3361 tacgaaggcg aaagaggctg aggccgagta caattcgaat tctgcggctc ttgccgcgac 3421 gaacctaaag aaagcccagg ccgagctccc ggccgtggcg gctgagtcca agcttcggaa 3481 gaagacggcc gatattgatg agaagtgggc cgagggtgac gcgtacctta accgcctggg 3541 cggtgtggcg gggaccctcg ggaccacggc aaagatgctc cgtgaagcga tcgggaagga 3601 tcgcaaggag aaggaaggtc gggaacggcg tcggacgttt gacgactggc tgaagggcac 3661 caacaggatg ccgaacgcaa taccggggga ttgatggaat tccgtacgag gttcacgaag 3721 aaagagcgga gcgggaaggt gtgcgccggg gggcgcacgt tgcaagcgtc gagggacgag 3781 tgcaacgtga acctcatcat ggccaggttc gagaagaccg gccagctccc gcagatgatc 3841 cggcgggatc cgcagtacgg ggaattcgcc tcggctcctg agtaccacga ggctatgtgc 3901 ctcgtggtga aggctcagga gcaattcgag gctctcccgg cacaggttcg gaaacgcttc 3961 gggaacgatc cggcggcgtt tttgggattc gcggtggatc cgcagaacca gcgggagctg 4021 gttcggatgg gtctggcgac cctgaacgag gatcacgcga agatggaatc cgcgaagctc 4081 gcggccgagt cgcggaagga ctcggaacag gaggaggagc ctcctccgaa aaaggccccg 4141 gtgaggagcg aagcgactcg ccgggggcgc gccgcaggcg ggggccccag cgcgtagagg 4201 ggggggtcag cccgtctcta cttgttataa tagggctgac tgacaccaac cgcaggttgg 4261 tgtcttaaga ggaggacgaa gcaatgcgag gacgacgtag aatgtcgaag cgcaggagtc 4321 ggagactctt tacccggacc gcgaagcggg tccaccgccg gaacggcggg ggtcgggtga 4381 tgcgtggagg cgttcggctt taggatctac gaaaaataga aaggaggcga gaca //