LOCUS MN582063 4363 bp DNA circular ENV 18-NOV-2019 DEFINITION Microviridae sp. ctQch27, complete genome. ACCESSION MN582063 VERSION MN582063.1 DBLINK BioProject: PRJNA540674 BioSample: SAMN11552893, SAMN11552892, SAMN11552894 Sequence Read Archive: SRR8996939, SRR8996941, SRR8996944 KEYWORDS ENV. SOURCE Microviridae sp. ctQch27 (freshwater metagenome) ORGANISM Microviridae sp. ctQch27 Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae. REFERENCE 1 (bases 1 to 4363) AUTHORS Malki,K. and Breitbart,M. TITLE Florida's Freshwater Springs JOURNAL Unpublished REFERENCE 2 (bases 1 to 4363) AUTHORS Malki,K. and Breitbart,M. TITLE Direct Submission JOURNAL Submitted (16-OCT-2019) College of Marine Science, University of South Florida, 830 1st St S, Saint Petersburg, FL 33701, USA COMMENT Submissions were annotated with Cenote-Taker2 available at https://github.com/mtisza1/Cenote-Taker2. ##Genome-Assembly-Data-START## Assembly Method :: SPAdes v. 3.12 Genome Coverage :: 7.951546x Sequencing Technology :: Illumina ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..4363 /organism="Microviridae sp. ctQch27" /mol_type="genomic DNA" /isolate="ctQch27" /isolation_source="Volusia Spring" /db_xref="taxon:2656699" /environmental_sample /country="USA" /collection_date="22-May-2017" /metagenome_source="freshwater metagenome" /note="genome binned from sequencing reads available in SRX5775735,SRX5775734,SRX5775731; metagenomic" CDS 1..324 /note="No HHsearch hits" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QGH72371.1" /translation="MYFPSEYSVLSLNRRSRGLGVVVDNRRWEPPGVVRRFRDVRGQV AKVAVSLRRPFRVPSALGFVNPRAVVVCVGRKARREVLFAMRKAGRGGMKRTRRRTEA SQIHC" CDS 337..1155 /inference="similar to AA sequence:UniProtKB:A0A0C5AG36_9VIRU" /codon_start=1 /transl_table=11 /product="putative minor capsid protein" /protein_id="QGH72369.1" /translation="MSWMAAGAQAAGSLLGAGVNLWEGSKNRKLQKEFAQQGIRWKVA DAQAAGIHPLYALGAQTHSFQPFMTGAGSELANMGQDVSRAIGATQTAGERAHTQAMA ALELDRAKLENELLRSQIARANAAPNPPLPGTPYLIDGQSGSGALVRNFPLDRVAPAP GQPSAEPGAITGLGYERTPSGWAPVPSGDVKQRIEDMLIPQAAWTWRNYILPSVGFMN PPSGVPLKKGYRWVMNPYSGFNYEQWPKWEERAVRNVKAWWKQSAVGRRWRGER" CDS 1287..2189 /inference="protein motif:PFAM:PF05840.12" /codon_start=1 /transl_table=11 /product="replication associated protein" /protein_id="QGH72370.1" /translation="MMRCRKPVLFRSGYARCGQCRSCRIDRRRIWAHRIMLEAKLSSA SSFVTLTYEDGKLPKSVGSDSWTLSPEHLQGWLKRIRRKVMPSKLRFFAVGEYGETTF RPHYHAAIFGGSACMYGVSQYSKVRTECCEWCQLVTKSWGFGNTFVGQLTDRSAAYIA GYVVKKMTHRDDWRLQAGQCPEFARMSLKPGIGAGVSSEVASLILEFDYEDVLEDVPS TLQHEMKELPLGRYLRRRIRAEIGWAPQAPRVVREKLLEEMRFVQKAAEHLGVATKEL VRQLWEPLAEAQERKDEILRRRKS" CDS 2186..3688 /inference="protein motif:PFAM:PF02305.16" /codon_start=1 /transl_table=11 /product="major capsid protein" /protein_id="QGH72368.1" /translation="MKRSVFSLSHHKLLSFDMGELVPIGMTEVLPGDTMQQATTMLVR ASPLLAPVMHPVDVRIHHWFVPHRLVWADFEKFITGGPDGLDASVFPTITFGGAIPAG TVMDYLGMPLGVAGLTVSALPFRAYNLIWNNWYRDQDLETALAVSTASGNDVTTNTNL KSCSWEKDYFTSARPWEQKGPTVTVPLGTTAPVMGLGFANTPSQMAASTVMRQTTPVA NETVNNPPWWMITNAANTMGVKADSAASGTTKPSVFADLTNASAVTVNTLRQALAFQR YEEARARFGSRYIEYLRYLGVKSSDGRLQVPEYLGGGRDTIQFSEILQTAADGTNPVG TMRGHGIAGMRSNRYRRFFEEHGYVMSLASVRPKTMYMQGLARTWNRRVKEDFWQKEL QFIGQQAIKNKELYAAHASPDGTFGYQDRYDEYRRSESSVSGEFRTVLNFWHFAREFG SSPALNGTFVSCVPTERTFAVSTNDVLWVMAKHSIQARRLVAKTGRSMTF" CDS 3890..4264 /note="No HHsearch hits" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QGH72372.1" /translation="MSLKRLRKRRISRLVRTRVWRIGSLTSILLRLRSTFAGRKPASL CMRKSSLMKKVSWFLLKGILKAFPRSRPRRRLLDGPRSPSRAAVGRSSRPLTGFRARL FLRLLGGVVGNAVHSPGELKAR" ORIGIN 1 atgtactttc ctagcgagta ctctgttctt tcgctgaacc gtcgtagtcg tggtctcggt 61 gtagttgtcg ataatcgtcg gtgggaaccg ccaggcgttg tacggcggtt ccgggatgtg 121 cgaggtcagg tggctaaggt ggccgtctct ttgaggcggc cttttcgtgt tccgtctgct 181 ttaggattcg ttaacccgcg agcggttgtt gtttgcgtag ggcgcaaggc tcgtcgggag 241 gtgctttttg cgatgcggaa agcgggccgc ggagggatga agcggacgcg gcgtaggaca 301 gaggcttcac agattcattg ctgataagag gttgagatgt cttggatggc agcaggggca 361 caagcagcgg gttcgttgct cggcgcaggg gtaaacctct gggaaggttc gaagaatcgg 421 aagttgcaga aggagtttgc gcagcaaggg atccgttgga aggtggcaga tgcgcaagcg 481 gcgggaattc atccgttgta tgcgcttgga gcgcagacgc actcgtttca gccttttatg 541 acgggcgctg gttcggagtt ggcgaacatg ggtcaagatg tatcgagggc gattggagca 601 acgcagacgg ccggggaacg tgctcacacg caagctatgg cagcgttgga gcttgatcga 661 gcgaagctcg agaatgagtt gttgcggtct cagatcgcga gggcaaacgc ggctccgaat 721 ccgccgttgc cgggtactcc gtacttgatc gatgggcagt ccggttccgg tgcgttggtt 781 cggaattttc cgttggatcg tgttgcgccc gcgccaggtc aaccttctgc tgaaccgggt 841 gctattactg gtcttggcta tgagcggacg cctagtggtt gggctccagt gccgagtggt 901 gatgtgaagc agcggattga ggacatgttg attccgcagg ctgcgtggac ttggcgtaat 961 tacattttgc cgtctgttgg ttttatgaat cctccgagcg gggtcccttt gaagaaaggg 1021 tatcggtggg tgatgaaccc ttatagtggg ttcaattatg agcagtggcc taagtgggag 1081 gagagggcag tccggaatgt taaggcgtgg tggaagcaga gcgcagttgg tcgtagatgg 1141 agaggagagc ggtaaatggc ttatcggaga aggagattgg gtcgcaggat gcgggcgcga 1201 gttggtagaa ggtttgtccg taggcggagg attgtgcgcc gtgggcggag cggcgtgcgg 1261 cgattgcgta ttgggtaccg gatgtgatga tgcggtgtcg aaagccggtg cttttcagga 1321 gcggctatgc tcgttgcggg cagtgccgct cctgtcggat tgataggagg cgaatatggg 1381 cacaccggat aatgttggag gcgaagttga gttcagcgag ttcatttgtg acgttgacat 1441 acgaggacgg gaagttgccg aagagcgttg gcagcgattc gtggacgctg tcgcccgagc 1501 acttgcaagg ttggctgaaa cggattcggc gcaaagtgat gccttcgaag ttgaggttct 1561 ttgcagtcgg ggagtacgga gagacgacct ttcggccgca ttatcacgca gcgattttcg 1621 gagggtcagc atgtatgtat ggagtaagcc agtatagtaa agtgcggacg gaatgttgtg 1681 agtggtgcca gttggttacg aagtcgtggg gatttgggaa tacgtttgtt ggtcagttga 1741 cggatcgtag tgctgcgtac attgctgggt atgttgtgaa gaagatgact catcgtgatg 1801 attggcggtt gcaggcagga cagtgtcctg agttcgcgcg gatgtcgttg aagccgggta 1861 ttggtgctgg tgtgtcgagt gaggtggcct cgttgatatt ggaatttgat tacgaggatg 1921 tgttggaaga tgttccgtct acgttgcagc atgagatgaa ggaattgccg ttgggtcgtt 1981 atttgcggag gcggattcgt gctgagatcg gttgggcgcc gcaggcgccg agggtggttc 2041 gggagaagct acttgaagaa atgcgttttg tgcagaaagc tgcggagcat ttgggcgtgg 2101 cgacgaagga attggtgagg cagttgtggg agccgttggc agaagcacaa gaacggaaag 2161 atgagatctt gaggcggagg aaatcatgaa gcggtcggtg ttcagtttgt ctcatcacaa 2221 gcttttgtcg tttgacatgg gagagctggt tcctattggg atgacggagg ttttgccggg 2281 tgatacgatg cagcaagcga ctacaatgtt ggtgcgagcg tctcctttgc tggcgcctgt 2341 catgcatccg gttgatgttc ggattcacca ttggttcgtg ccgcatcggt tggtgtgggc 2401 ggattttgag aagttcataa ctggcggtcc tgacggactt gatgcgtcgg tgtttccgac 2461 gatcacgttt ggtggagcga taccggcagg gacggtgatg gattatttgg gcatgccatt 2521 gggtgttgct gggttaacgg tctcggcgtt gccgtttcgt gcgtacaact tgatatggaa 2581 taactggtat cgggatcagg atttggaaac ggcgttggcg gtttctacgg cgtcgggaaa 2641 tgatgtgacc acgaacacga acctgaagtc atgttcgtgg gagaaggatt attttacgtc 2701 tgctcggccg tgggaacaga aaggcccgac tgtgacggtg ccgttgggga cgaccgctcc 2761 tgttatgggg cttggttttg caaacacacc ttctcagatg gcggcgtcta cggtgatgcg 2821 tcagacaact ccggtggcga atgagacggt gaacaacccg ccgtggtgga tgattacgaa 2881 tgcggcgaac acgatgggtg tgaaggctga tagtgctgct agtgggacga ccaagccctc 2941 ggtgtttgct gatttgacga acgcgtctgc ggtgacggtg aacacgttgc ggcaggcttt 3001 ggcgtttcaa cggtatgagg aggctcgcgc tcggtttgga tctcggtaca tcgaatatct 3061 gcgttatctt ggtgtgaagt catcggatgg tcgtttgcag gttcccgagt atctgggagg 3121 tggtcgtgac acgattcagt tctcggagat tttgcagact gcggcggacg ggactaatcc 3181 tgttggtact atgcgcggtc acgggatcgc cggaatgcgt tctaatcgtt atcgtcgttt 3241 tttcgaggag catggttatg tgatgtctct cgcttcagtg cgtccgaaga cgatgtatat 3301 gcaagggttg gcgcggactt ggaatcggcg tgtgaaggag gatttttggc agaaggaact 3361 tcagttcatc ggtcagcaag cgattaagaa caaggagctt tatgcggctc acgcctcgcc 3421 ggatgggacg tttgggtatc aggatcggta tgatgagtat cggcgttcgg agagttcagt 3481 gtcgggtgag tttcggacgg tgctgaattt ctggcacttt gctcgtgagt tcgggtcttc 3541 tcctgctttg aatggtacgt ttgtttcgtg cgttccgacc gaacggacgt tcgcggttag 3601 tactaatgat gtgttatggg tgatggcgaa gcattcgatc caagcgcggc gcttggtggc 3661 taagactggt cggtctatga cgttttgatg gaggctgtgg tgaagatttt ggcagggaaa 3721 gaagtgcaga ctgtgagtga gaagtcgaat cttggtcgtt tggatgagcg tgggcgggag 3781 gttgttgacg ggcggcctat gcagccgcct ttgggtttga agcctacgcc tgacttgatg 3841 gaacagattc ggcggatgat cgccgtggag cgggaaatgc aagggagtga tgagcctgaa 3901 acgtttgagg aagcgtcgga tttcgagact ggtgaggaca cgggtttgga ggattgggag 3961 ccttacttcg atcctactcc ggttgaggag tacattcgcc ggcagaaagc cggcgagcct 4021 ttgcatgaga aagtcgagct tgatgaagaa ggtgagttgg ttcctgttga aggggatcct 4081 gaaagcattc ccccggagcc gcccaagaag gcgccttctg gacgggccaa gaagcccgtc 4141 tagggcggcc gtaggccgct cgtctcgacc cttgacgggc tttcgggccc gtcttttttt 4201 gcgcctgttg ggcggcgtag tggggaatgc ggtgcattcc cctggtgagc tcaaagctcg 4261 ttgacagtac gttactcgat acgtactgtg ctaggtgaca cctgattcgg aggttatcgt 4321 ggcccgaggc cggaatcggc aaggacgtgt tcggaaagtc aaa //