LOCUS MN582054 4250 bp DNA circular ENV 18-NOV-2019 DEFINITION Microviridae sp. ctYqV29, complete genome. ACCESSION MN582054 VERSION MN582054.1 DBLINK BioProject: PRJNA540674 BioSample: SAMN11552893, SAMN11552892, SAMN11552894 Sequence Read Archive: SRR8996939, SRR8996941, SRR8996944 KEYWORDS ENV. SOURCE Microviridae sp. ctYqV29 (freshwater metagenome) ORGANISM Microviridae sp. ctYqV29 Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae. REFERENCE 1 (bases 1 to 4250) AUTHORS Malki,K. and Breitbart,M. TITLE Florida's Freshwater Springs JOURNAL Unpublished REFERENCE 2 (bases 1 to 4250) AUTHORS Malki,K. and Breitbart,M. TITLE Direct Submission JOURNAL Submitted (16-OCT-2019) College of Marine Science, University of South Florida, 830 1st St S, Saint Petersburg, FL 33701, USA COMMENT Submissions were annotated with Cenote-Taker2 available at https://github.com/mtisza1/Cenote-Taker2. ##Genome-Assembly-Data-START## Assembly Method :: SPAdes v. 3.12 Genome Coverage :: 93.684706x Sequencing Technology :: Illumina ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..4250 /organism="Microviridae sp. ctYqV29" /mol_type="genomic DNA" /isolate="ctYqV29" /isolation_source="Volusia Spring" /db_xref="taxon:2656700" /environmental_sample /country="USA" /collection_date="22-May-2017" /metagenome_source="freshwater metagenome" /note="genome binned from sequencing reads available in SRX5775735,SRX5775734,SRX5775731; metagenomic" CDS 1..897 /inference="protein motif:PFAM:PF05840.12" /codon_start=1 /transl_table=11 /product="replication associated protein" /protein_id="QGH72132.1" /translation="MKCSEPVCIDGKALPCGRCLCCSINRRRLWTHRLMLEAKVCIAT SFLTLTYRDESVPRLKDGSQSLCKKHLQDWLKRFRKRTSPMRWRFYGIGEYGDATQRP HYHVALFGQVSCEYGRSRYSKVQKNCCEFCDTVRDTWGHGLIDCGELNAQSAAYICGY VLKKMTKVDDVRLQGREPEFCRMSNRPGIGFDAVADVADALLSVDADEDLVDVPNVLR HGRSCYPLGRYLKGKLREQIGRCTAVPEVVKKKMEEEMRPLREAAFYASRSFAEVVAE VSAGEVARVEGRNRVFRGRKVL" CDS 894..2399 /inference="protein motif:PFAM:PF02305.16" /codon_start=1 /transl_table=11 /product="major capsid protein" /protein_id="QGH72133.1" /translation="MKRSKFSLSNYKLLSCDMGELVPCGLTEVLPGDTIQQATSCLLR ASPLLSPVMHPVDVRLHHWYVPHRLVWEDFEDFITGGSDGLDASVFPTITIGGGSGAA VGSLADYLGVPTGVNNIEVSALPFRGYAMIWNEWYRDQDLQTALAISEGSGADTTTST VLQNCSWEKDYFTSSRPWEQKGPAVTVPLGDTAPVDGISMRNLGNAANNAGQSYGAQA ASDYGPGTTPTWRADTQELYIRAQSNAVSGTGNRPQIFADLSNASAITVGALREAMAL QRYEEARARFGSRYVEYLRYLGVQSSDARLQRPEYLGGGRETIQWSEVLQTAEGTDPV GSLKGHGISSMRSNRYRRYIEEHGYVYTFLSVRPKTIYAQGLARHWNRRVKEDFWQKE LQFIGQQEVLNKEVYAAHASPNNVFGYQDRYDEYRRAESTIAGEYRSVLDFWHMARIF GSSPALNGDFVKCVPTERTFAVPSEDVLWCMVKHSIQARRLVAGVGKSFIY" CDS 2901..3287 /note="No HHsearch hits" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QGH72134.1" /translation="MASKSKSKRMRSVRRIVNVNTTRFSVLDPVLERAITQDLLDNRA FSPSPLRRLRTLLGTPARLRARLISKVDPVSYVSPYLDTVNPLKAVHCVRRKIRRQVI FALGKSGKGAGPRKRPRRTDTSDLRC" CDS 3288..4121 /inference="similar to AA sequence:UniProtKB:A0A0C5AG36_9VIRU" /codon_start=1 /transl_table=11 /product="putative minor capsid protein" /protein_id="QGH72131.1" /translation="MWEALGSVAGSLLGGLFGSSEADRQADLQKEFAQKGIQWKVADA KKAGIHPLYALGAPTMSYSPITTGMGDAIQNAGQSLGRAVDANLSAGERLDGFTKASQ ALQLERGKLENELLKVKIASETAVTAGQLGPAAPSPESAYLIPGQGQTVLKGRTYNQV QTDPLKRQSVSSVSPNQEAGAIAEAGFMRTPTGYAPIRSKDAMDRMDDDWAGSLAWNL RNRLLPMWDSSGRTPPIKLRKGYYWYWNPVLQEYQVAKRIPESVTDGPSRPLRLDYRR K" CDS 4078..4248 /note="No HHsearch hits" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QGH72135.1" /translation="MVRLGRYGWIIGGSEMRRGFKKRFARGHKRRGRGVRRRSNYQRR KGARGVRIGFRM" ORIGIN 1 atgaaatgtt cagagcccgt ttgtattgat ggaaaggcac ttccgtgtgg gaggtgcctt 61 tgttgttcaa ttaataggag acggttatgg actcatcggt tgatgttaga agcgaaggta 121 tgtattgcaa cgtcgttttt gacgttaacg taccgggacg agagtgtacc aaggttgaag 181 gacggttcgc agagcttgtg caagaagcat ttgcaagatt ggttaaagag gtttcgcaaa 241 agaacgagtc ctatgaggtg gaggttttat gggatcgggg aatatggaga cgcgacccag 301 agaccacatt accatgtggc tttatttggg caggtgagtt gcgagtatgg ccgttcaagg 361 tactcgaaag ttcagaaaaa ttgttgtgag ttttgcgata cggtcagaga tacgtggggt 421 catgggttga ttgattgtgg cgagttgaac gctcagtctg cagcttacat ttgtgggtat 481 gtattgaaga aaatgactaa ggtggatgat gttcggttgc aaggtcgaga gccagaattt 541 tgtcggatgt cgaataggcc aggtattggg tttgacgctg tggctgatgt ggctgatgct 601 ttgttgtctg tcgatgcgga cgaagatttg gtggacgttc ctaatgtgtt gcgtcatggg 661 cgttcttgtt atcctttggg acgctactta aagggaaagt tgcgtgagca aattgggcgg 721 tgcacggcag taccagaagt ggttaagaaa aaaatggaag aggaaatgcg gcctttgcga 781 gaagctgcat tttatgcttc gcggtcgttt gcggaagtgg tggcggaagt gagcgcgggt 841 gaagttgccc gcgtggaagg aagaaaccga gtttttagag gaaggaaagt tctatgaaga 901 ggtctaagtt ttcactgtcg aattataagt tgctgagttg tgatatgggg gagttggtgc 961 cctgcggact gacggaagtg ttgccagggg acacgataca gcaagcaacg tcgtgcctgt 1021 tgcgagcgag tccgttgttg tctccggtga tgcaccctgt agatgtgaga ttacatcatt 1081 ggtatgtgcc gcatcggtta gtgtgggagg attttgagga ttttattact ggtggatcgg 1141 acggtttgga cgcttcggtt ttcccaacta ttactattgg tggtggttcc ggcgcagcgg 1201 ttgggtccct ggcggattat ttaggcgtgc ctactggtgt taacaatatc gaagtgtcgg 1261 cgctgccgtt tcgtggttac gcgatgatct ggaatgaatg gtatcgcgat caggatttac 1321 aaacggcgtt ggctatttca gaggggtcgg gggcggatac tacaacgtca acggtgctgc 1381 agaattgttc atgggaaaag gattatttta catcgtcgcg accgtgggag caaaaagggc 1441 cagctgtgac agtgccgctc ggcgatacgg ctcccgttga tgggattagt atgcgtaatt 1501 tgggtaatgc ggcgaataac gcaggtcaaa gttacggcgc ccaggcggcc tcggattatg 1561 gacctggaac gacgccaacc tggcgtgcgg atacccagga gctgtatata cgtgctcaga 1621 gtaatgcggt gtctggcact ggtaatagac cgcagatatt tgcggatttg tctaacgcgt 1681 ctgcgattac tgttggagcg ttgcgagagg ctatggcgtt gcaacgatat gaagaggcaa 1741 gagccaggtt tggttctagg tatgtggagt atctcaggta tttaggtgtt cagagttcgg 1801 atgctcggct acagcgacca gagtatttag ggggtggacg tgagacgata cagtggtcgg 1861 aggtgttgca gactgctgaa gggactgatc cggtcggttc gcttaagggc catggtattt 1921 cgtctatgcg gtctaatcgg taccggcgtt acattgaaga gcatggttac gtgtatacgt 1981 ttttgtccgt gaggccgaag acgatttatg cgcaagggct tgcgagacac tggaatagac 2041 gcgtgaagga agatttttgg caaaaagaat tgcagtttat tggccagcag gaagtcctta 2101 ataaagaggt gtatgcggcg catgcgtcgc ccaataatgt ttttggctat caagataggt 2161 atgatgagta tcgtagggcc gagagcacta ttgctggcga gtataggtcc gttttggatt 2221 tttggcatat ggcaaggatt tttggttcat ccccggccct aaatggtgac tttgtaaagt 2281 gcgtgcctac agagcgtacg tttgcggttc cgtccgagga tgtgttgtgg tgtatggtga 2341 agcattctat tcaggcgcgc cggctggttg ccggtgttgg taagtcgttt atttattgag 2401 cgaggtgtat gtgatgaaga ttaagcaagg tttggaagtt gactcgaagt ccgtgaagtc 2461 caacaaaggt aggttggatg ctcgcggccg tgagttgctg gatgggaggc caatggagcc 2521 tccggttggt tttgtgcgac ggccgtccct ggcggagacc attcgggcta tggtacggca 2581 agagaagttg cagcaagagt tggcctccgc cggcgcagag acgtttgaag aggcggatga 2641 tttttacgtt gaggatggat ttgagcctag aagccaatat gagctgctag agcgggattt 2701 gctcgccgaa gaggcagaag ttcccccaga gaagccgccg gcgcaggcgg agcctccaag 2761 cgaagcgaag ccggcggccc ctacgggtga ctctaagggt taacgctgct ggtggcgacc 2821 tggctgagcc aggtcgccga tctcgcacag tacactgcct tgttgtgtac tgtgctaggt 2881 gacaccggga ccctgttaaa atggcctcaa agtctaaaag taagcgaatg cgatcggttc 2941 gacgtattgt caatgtcaat actacgcggt ttagcgtgct cgatccggta ttagagaggg 3001 ccattacaca ggatttgcta gacaataggg cgttttcgcc gtcgcccctg cggcggttgc 3061 ggacgttgtt gggtacgcct gcgcgattac gggccaggct gataagcaaa gttgatccgg 3121 tgtcttacgt aagtccttat ttagatacgg ttaatccgct gaaagcggtg cattgcgtcc 3181 ggagaaaaat ccggcgccag gtcattttcg cattgggaaa aagtggtaaa ggcgcgggac 3241 cacggaaaag accgcgccgt actgatacat cagatttgag gtgttagatg tgggaagctt 3301 taggatctgt tgcaggaagc ttgttaggtg gtcttttcgg ctcaagtgag gcggacaggc 3361 aagcggacct gcagaaggaa tttgctcaaa agggaattca gtggaaggtg gcggatgcta 3421 agaaggctgg tatccatcca ttatacgctt tgggggcacc tacgatgtcg tacagtccga 3481 ttacgactgg gatgggcgat gcgatacaga atgcgggtca gagccttggg cgagcggttg 3541 atgcaaatct tagtgctggt gagcgtttgg atggttttac gaaagcttct caggctctgc 3601 agctagagcg cggtaaactt gaaaacgagt tattgaaagt gaagatagct agtgagactg 3661 ccgtcacggc agggcagtta ggtccagctg cgcccagtcc cgagagtgcg tatttgatac 3721 cgggtcaggg tcagactgtg cttaaaggta ggacgtataa tcaggttcag actgatccct 3781 tgaaaagaca gtctgtatcg tctgtcagtc caaatcagga ggctggtgct atagctgagg 3841 cggggtttat gagaacgccg actgggtatg ctccaatacg ctcgaaagat gcaatggacc 3901 gtatggacga tgactgggcg ggttcgttgg cgtggaattt gaggaatagg ttgcttccta 3961 tgtgggattc aagtggtcga acaccgccaa ttaagttgcg taagggttat tattggtatt 4021 ggaacccagt cttgcaggaa tatcaggttg cgaagagaat tccagagtcg gtgacggatg 4081 gtccgtctcg gccgttacgg ctggattata ggaggaagtg aaatgcgtag aggatttaag 4141 aagcggtttg cgcgcgggca taagcgtcgc ggccgtggtg taagacgtcg gtcgaattat 4201 caacgccgga agggtgctcg tggagtacgc attggtttca ggatgtagta //