LOCUS MH617735 4481 bp DNA circular ENV 29-MAR-2019 DEFINITION Microviridae sp. isolate ctbb130, complete genome. ACCESSION MH617735 VERSION MH617735.2 DBLINK BioProject: PRJNA393166 BioSample: SAMN09074552 Sequence Read Archive: SRR7122375 KEYWORDS ENV. SOURCE Microviridae sp. (animal metagenome) ORGANISM Microviridae sp. Viruses; Microviridae; unclassified Microviridae. REFERENCE 1 (bases 1 to 4481) AUTHORS Tisza,M., Buck,C., Pastrana,D., Welch,N. and Peretti,A. TITLE Uncovering a Universe of Circular DNA Viruses in Animal Metagenomes JOURNAL Unpublished REFERENCE 2 (bases 1 to 4481) AUTHORS Tisza,M., Buck,C., Pastrana,D., Welch,N. and Peretti,A. TITLE Direct Submission JOURNAL Submitted (12-JUL-2018) Lab of Cellular Oncology, National Cancer Institute, 37 Convent Dr., Room 4118, Bethesda, MD 20814, USA COMMENT On Mar 29, 2019 this sequence version replaced MH617735.1. ##Genome-Assembly-Data-START## Assembly Method :: SPAdes v. 3.11.0 Genome Coverage :: 14.750502x Sequencing Technology :: Illumina ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..4481 /organism="Microviridae sp." /mol_type="genomic DNA" /isolate="ctbb130" /isolation_source="minnow tissue" /db_xref="taxon:2202644" /environmental_sample /country="USA" /collection_date="01-Jun-2017" /metagenome_source="animal metagenome" /note="genome binned from sequencing reads available in SRX4043871; metagenomic" CDS 1..1590 /inference="protein motif:CDD:222784" /codon_start=1 /transl_table=11 /product="major capsid protein" /protein_id="AXH78032.1" /translation="MLGNRYSQHSFAQIPDVKMARSQFDRSFTVKDTFNFDYLIPIFV DEILPGDTCNVRVNTFARLATQLVPIMDNLYIDFFFFFVPNRLVWNNWEKFNGAQTNP GDSTSYVLPSLDAFPAGGPEVDTIYDKFGLPTDVSGAWTLGNTLPLRCYNKIWNAWFR DENLQNSVIDNMDDGPDQIADYTLLKRGRRHDYFTSALPWPQKGAAVSLPLGTSAPIL RTSGAPNAWIAYQKNTNTTTTVDGEIRANNAGAVYQGGSSAELSFDPMGGLYTDLSNA TAATINSLRQAFQVQGLLELDARGGTRYVEILQAHFNVISPDFRLQRPEYLGGGQTKI NSHPVAQSAPTSGSNYFAQLGAFATSSTMGSNIGFTKSFVEHGYVIGLACARADITYQ QGLERFWSRSTRYDFFWPKLQELGEQTILNKEIYLQGNGSDTAVFGYQERYAEYRYKP SQIHGQFRSTYANSIDQWHMAEEFGSLPALNSTYIVQNTPIVRALASSTAPQILFDAF FSYKHARPMMTYSVPVTLGRF" CDS 1590..2330 /inference="protein motif:CDD:164789" /codon_start=1 /transl_table=11 /product="DNA pilot protein" /protein_id="AXH78033.1" /translation="MWETALAAGASGVLGYLGQRSANDANKDIAASAQEANMGEAARN REFQQASADKAMAFSDAQASRQMAFQGSQTAQQQAFQERMSSTAYQRGVADLKAAGLN PILAGMQSGASTPSGASASGASGSASSASGAQGSAVTARMENTMGGFGAVIRDIVDIK RAEADIKLTNAQRDKTLIDAGKSKSETDLLKNQKNETDLSSDFYGFVKKALEKAKESL GSGAKSPERKRFDDNNNAIQNYNRMIRP" CDS 2327..2815 /inference="protein motif:CDD:164781" /codon_start=1 /transl_table=11 /product="internal scaffolding protein" /protein_id="AXH78034.1" /translation="MIDPVQKITKRADGRVRVQTINEEPSKTQQQFKDECDINNIVKK FSTTGEFTHLTSKEGRYADFSQITDYRDMLDTVLYAQEAFASLPADVRLRFQNNPAEL LEFVQNPKNYDEGVKLGLVQPRAQGSNVNTNDLNDLKTTEPKAQKTKSQKTNDSTPAS PE" CDS 3058..3309 /inference="protein motif:CDD:222780" /codon_start=1 /transl_table=11 /product="nonstructural protein" /protein_id="AXH78030.1" /translation="MELKMFSIRDSKGEVFNPPFYKKTHGEAERDFQTLCKDEKSTVN QYPEDFDLYYIGTYDDNSGKIQSLDTPQHLVKAVNVLNR" CDS 3534..4466 /inference="protein motif:CDD:222782" /codon_start=1 /transl_table=11 /product="replication initiator protein" /protein_id="AXH78031.1" /translation="MRCTRPRTVGFMADGRTIAWSPKQYIKEYATFQLPCGQCLSCRL EYARQWAVRCVHEAQMHERNSFITLTYSDENLKSTKLIYRDFQLFMKRLRKIQDEPIG VFVTGEYGDQTKRPHWHAIMFNYSPRDAEKKYQTAHGHQVFESESLSSLWGHGLCEFG NVTFESAGYCARYAAKKLGHGIDGSHGYDPISKKSSKYAIGKKWLEKYWYTDCFAQGE IVLPGGRGTTTIPRYYEKWLKDTHPDAWLRYVTEVKIPKMEKSALRAEQENAEYFQTL YARHPSKSNPLTSHQVRDVILKSKFKVLQDNLKL" ORIGIN 1 atgttaggaa accggtactc tcagcacagc tttgctcaaa ttcccgatgt caagatggct 61 cggtctcagt tcgatcggtc ttttacggta aaagatacgt tcaacttcga ttatttaatt 121 ccaatattcg tcgatgaaat tttgcctggc gatacgtgta acgtaagagt taacacgttc 181 gcacgtcttg caactcagct cgtaccgatt atggataatc tttatatcga tttcttcttc 241 ttcttcgtgc caaaccggct cgtatggaac aattgggaaa aattcaatgg tgcacaaaca 301 aatccgggtg attccacaag ttacgtactt ccgtcgctgg atgcgttccc agccggcggt 361 ccagaggtcg atacgatata cgataaattt ggtttaccta cggatgtttc tggagcttgg 421 acgttgggaa acactcttcc tctcagatgt tacaataaaa tctggaacgc ttggtttcgt 481 gacgagaacc ttcagaattc cgttatcgat aacatggacg acggaccgga tcagatcgcg 541 gattatactc ttctcaagcg agggcgccgc catgactatt ttacctcggc gctgccttgg 601 ccacagaagg gagctgccgt atcacttcca cttggaactt cggctcccat tcttcgtact 661 tcaggcgctc cgaatgcttg gatcgcttat caaaagaata ccaacactac gacgacagtt 721 gacggcgaga ttcgcgctaa taacgcgggc gccgtttacc aaggtggctc ttcggctgag 781 ctctcttttg atcctatggg cggcctttat actgatttgt ctaacgctac tgcggctact 841 atcaattcct taagacaagc tttccaagtt caaggtcttc ttgaactgga tgcacgtgga 901 ggcacgagat atgttgaaat tcttcaagct cacttcaacg tcattagccc agacttccgc 961 ctccagcgtc ccgaatacct cggcggcggc cagacaaaaa ttaattccca tccggtcgca 1021 cagagcgcac caacctcagg atcaaactac ttcgcacaac tcggcgcttt cgcgacttca 1081 agtacgatgg gatctaatat cggattcact aagtcgttcg tcgaacacgg atacgtcatc 1141 ggacttgcat gcgctcgagc cgacatcacg taccaacaag gcttagagag attctggtct 1201 cgttctactc gctacgactt cttctggccg aaacttcagg aacttggcga acagacgatt 1261 cttaataaag agatttatct gcaaggtaac ggttccgaca ccgcggtgtt cgggtatcag 1321 gagcggtacg ctgagtaccg ctacaagcct tcgcaaattc acggccaatt ccgctccact 1381 tatgcgaact cgatcgatca gtggcatatg gctgaagagt tcggatcttt gccggctctg 1441 aattcgacct acatcgttca gaacactccg atcgttcgcg ctctcgcgtc gagcactgct 1501 cctcagattc ttttcgatgc tttcttctcc tacaaacatg cacgccctat gatgacctac 1561 agcgtgcccg taacccttgg gaggttctaa tgtgggaaac tgctttagcc gcgggcgctt 1621 ctggcgtcct tggctatctg ggtcaaaggt ctgccaacga cgccaataaa gacattgctg 1681 cgtcggcgca agaggccaat atgggagagg cagctagaaa ccgtgagttt caacaagcct 1741 cggctgacaa agctatggct ttctcggacg cacaggcttc tcgccagatg gcctttcaag 1801 gctctcagac ggcccaacag caggccttcc aagaaaggat gagctccaca gcctatcagc 1861 gcggcgtagc cgacctgaag gctgctgggc tcaatccaat actggccggc atgcagtccg 1921 gcgcttctac tccatccggt gcttctgcga gcggtgcgtc aggctccgct tccagcgctt 1981 ctggagcaca gggaagcgct gttacggcac ggatggaaaa tacaatggga ggtttcggag 2041 cggttatccg cgatatcgtc gatattaaga gagcggaggc tgatattaag ctcaccaatg 2101 ctcagcgtga taaaacgctc atcgacgcag gaaaatctaa atccgaaact gaccttttaa 2161 aaaaccagaa gaacgagact gacttatcgt ccgattttta cgggttcgta aaaaaggcgc 2221 ttgaaaaagc aaaggagagt ctcggttctg gtgctaagag tcccgaacgc aaacgctttg 2281 atgacaacaa taacgctatt caaaactata acaggatgat tcgaccatga ttgatccagt 2341 acagaagata acaaaacgtg ccgatggcag agttcgcgta caaacaatta atgaggaacc 2401 ttcaaaaacc caacagcaat tcaaggatga atgcgacata aacaacattg taaaaaaatt 2461 ctctactacc ggtgaattca ctcaccttac atctaaagaa ggtcggtacg ccgacttctc 2521 tcaaatcact gactacagag acatgctgga cactgttctc tatgctcaag aggctttcgc 2581 ctctctacct gcagacgtac gtctgcgctt tcaaaacaac cctgccgaac ttttagagtt 2641 cgtgcaaaat ccaaaaaact acgacgaagg agtaaaacta ggattagtac aacccagggc 2701 tcaaggttca aatgtaaaca caaacgattt aaacgatcta aaaacaacag aacccaaagc 2761 ccaaaaaact aaatcccaaa aaacaaatga ctcgactccg gcttcgccgg agtgagtaag 2821 caagcggtag cgcgctagga gtgcgggcta caacagagtc aaaaccgcac aacaacgtag 2881 gcgagcttgc gaagccgact acggagtggg ttcgggagag ggcgaagctc tctcccgact 2941 cacaaaaaac caaacataaa ataaacttct aaaattaaac tagacaaaca gcctgaagtt 3001 accaaacttc aggaaccgat aaccgggcag cgatgcccat aaccgaaggt ggtcaacatg 3061 gaactgaaaa tgttctcaat acgcgattcg aagggtgaag tattcaaccc tccgttctac 3121 aaaaaaacgc acggcgaagc cgagcgtgat ttccagactc tctgcaaaga tgagaagtct 3181 accgtcaatc aatatccaga ggacttcgac ctctactaca tcggcacata tgacgataac 3241 tcagggaaga tccaatccct ggacacacca cagcatttag ttaaggctgt gaacgttctt 3301 aaccgataac gcacccgggc ctaattacgc ttccttgttg taattaggcc cactgacaca 3361 ctacactgtg tcaaacaaca aaaacgcaaa ggattccgga tgcgccgtaa gccaatgtcg 3421 aacaaatctt ctcgtaaaca tttccgcaaa aatactggcg tccataacgt caataatctg 3481 aatccgcgga aatttcgcgg cggcatcaga ctgtaaaaaa aaggcgaata cagatgcgct 3541 gtacccgccc taggaccgta ggcttcatgg ccgatggtcg gaccattgcc tggtccccaa 3601 aacaatatat caaagaatac gcaacctttc aattgccgtg cgggcagtgc ctttcctgtc 3661 gtctcgagta tgcgagacaa tgggcagtac gctgcgtaca cgaggcacaa atgcacgagc 3721 gtaattcttt cattacgctt acttattcag acgaaaatct caaatcaaca aaacttattt 3781 atagagactt tcagctattc atgaaacgtc ttagaaaaat tcaggacgaa ccaataggtg 3841 tgttcgtaac aggagaatat ggtgaccaaa cgaaaaggcc tcactggcac gccattatgt 3901 ttaactacag cccgcgggac gctgagaaaa agtatcaaac agctcatggg catcaagtat 3961 ttgaatcaga gtctctctcg agcctatggg ggcatggact ctgtgaattc ggtaacgtca 4021 cttttgaatc agcaggctat tgcgctcgat acgctgcgaa gaagcttgga catggtatcg 4081 atggatctca cggctatgat ccaatatcaa aaaaatcttc aaagtacgct atcggaaaaa 4141 aatggctcga aaagtattgg tacaccgact gcttcgctca aggagaaatc gttctcccag 4201 gtggtagagg aacaactact attcccagat actacgagaa atggctgaaa gacacgcatc 4261 cagatgcgtg gttacgttat gtaacagaag tcaaaattcc gaaaatggaa aaatccgcgc 4321 ttcgcgcgga acaggaaaac gcagagtatt tccagactct ctacgctagg catcctagta 4381 aatcaaatcc attgacctcg caccaggtgc gagatgtcat tcttaaaagt aaattcaaag 4441 tgcttcagga taatctgaag ctataaacaa cagaggtggt c //