LOCUS MH617237 4891 bp DNA circular ENV 29-MAR-2019 DEFINITION Microviridae sp. isolate ctbb630, complete genome. ACCESSION MH617237 VERSION MH617237.2 DBLINK BioProject: PRJNA393166 BioSample: SAMN09074552 Sequence Read Archive: SRR7122375 KEYWORDS ENV. SOURCE Microviridae sp. (animal metagenome) ORGANISM Microviridae sp. Viruses; Microviridae; unclassified Microviridae. REFERENCE 1 (bases 1 to 4891) AUTHORS Tisza,M., Buck,C., Pastrana,D., Welch,N. and Peretti,A. TITLE Uncovering a Universe of Circular DNA Viruses in Animal Metagenomes JOURNAL Unpublished REFERENCE 2 (bases 1 to 4891) AUTHORS Tisza,M., Buck,C., Pastrana,D., Welch,N. and Peretti,A. TITLE Direct Submission JOURNAL Submitted (12-JUL-2018) Lab of Cellular Oncology, National Cancer Institute, 37 Convent Dr., Room 4118, Bethesda, MD 20814, USA COMMENT On Mar 29, 2019 this sequence version replaced MH617237.1. ##Genome-Assembly-Data-START## Assembly Method :: SPAdes v. 3.11.0 Genome Coverage :: 9.296667x Sequencing Technology :: Illumina ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..4891 /organism="Microviridae sp." /mol_type="genomic DNA" /isolate="ctbb630" /isolation_source="minnow tissue" /db_xref="taxon:2202644" /environmental_sample /country="USA" /collection_date="01-Jun-2017" /metagenome_source="animal metagenome" /note="genome binned from sequencing reads available in SRX4043871; metagenomic" CDS 1..1617 /inference="protein motif:CDD:222784" /codon_start=1 /transl_table=11 /product="major capsid protein" /protein_id="AXH75807.1" /translation="MKSVMRHQFSQVPRAEIPRSSFDRSHGYKTTLDAGYLYPIFVDE ALPGDTFNLRLTTFARLATPIKPFMDNLHADVFFFAVPYRLVWDNFKKFFGEQNNPAD STSFLVPQFTAHKPVTGSLSDYMGLPVDGTSPVLAGNITYNSLHHRAYNLIWNEWFRD QNMQNSVTVDKGDGPDNIANYVLLRRGKRHDYFTSALPWPQKGVAVSIPLGTSAPVKT SSTQQFNTNGASALVFRDLNNVLPGASNSIGASNVTGAAFLSNTAATASAQFLAPSNL YADLSTATAATINSLRQAFQIQKIYERDARGGTRYTELIQSHFGVTSPDARLQRPEYL GGGSTAINVNPIAQTGQTGLTGGGTPQGNLAAMGTFHHSGPGFSKSFTEHCLILGLVN IRADLNYQQGMNRMWTRQTRFDFYWPALAHIGEQAILNQEIYCVGTPAQDTAVFGYQE RFAEYRYKPSIITGQFRSTFATTLDVWHLAQNFASLPALNSTFIVDNPPLARVVAVPT QPMFLMDCFFNLRCARPMPVYGVPGNIDRF" CDS 1627..2487 /inference="protein motif:CDD:164789" /codon_start=1 /transl_table=11 /product="DNA pilot protein" /protein_id="AXH75808.1" /translation="MEPLTTALITGGASLLGNVFSSAMNAGQSHDNTMANIANQQWAL GESERFNQEQADLQRQWSASQINQQQAFQERMSGSAFQRASADMQKAGINPIMAFGNP ASSPSGASGGGQSASVSTPSMPQFTEQSKFSGFSSLGDAVSKAMSSAVQVKTMDKMAE EMAEIKARETKEKAETLSELKRPALIEAQTSTEHKRPANIEAQTATEQKKPASVEAQT AQTLTEERLRRYGLEQAAFQEKSAKDLNQMSDEGRKTLNVTSWGMQKVMDILAPFLST ARTANQTLRR" CDS 2491..2979 /inference="protein motif:CDD:164781" /codon_start=1 /transl_table=11 /product="internal scaffolding protein" /protein_id="AXH75809.1" /translation="MSTKSFPQASHDFIAYNHTDTAVWNHEPSLTRQEFAEECDINAL MARYNNHVIGGPGNLAPSEPMYFDFADMPQDLMGYMQFMEDCEKSFMSLPAIVRKEFD NSALEFVAFASDPTNLQQMRDWGLAPPAKVNPAPGAAADASNSSPPSAAPPPSQSAPS AS" CDS 2912..3172 /note="No conserved domains" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AXH75802.1" /translation="MRLIPLRLRPPRPLLRARLARPETRQHICLLDTYVLSDSQHWSG GPIMKRHKMSSSHSKRSFSKGASYTHKKNMPSRLPMRGGIRL" CDS 3298..4107 /inference="protein motif:CDD:222782" /codon_start=1 /transl_table=11 /product="replication initiator protein" /protein_id="AXH75805.1" /translation="MDKANQWGLRCLHEKKMWKWNFFATLTYDDESLPPGGTLCLRDV QLFMKKLRFAKNSTVENPVRFFLAGEYGDVNKRPHYHALLFNCPFTDLVRWSSGKRGE PLYTSRELSELWGKGHCSLGEVTYESAVYCAKYGLKKLKVNADSPPAIREEYERRYVV YDADGVVYERVPEFAVMSRRPGIGADYYNKYGKEVRDHDSVVVSGRKVRPPRFYDGRS EVVDPERFEELKAARKREAVKEVNKADNTPERLRVKEEILKAAEKRKERNL" CDS 4107..4406 /inference="protein motif:CDD:222780" /codon_start=1 /transl_table=11 /product="nonstructural protein" /protein_id="AXH75806.1" /translation="MKLEVFTVYDRQINAHMQPFFCRTKGEAIRSFTEAVNDPSKPFG KYSTDYSLMCLGEFDDGSGTFVCHDPVRVISANEVIEATSPTAEDPPPVLRRLPM" ORIGIN 1 atgaagtccg ttatgcgtca ccagttttct caggttccgc gtgctgagat tccgcgttct 61 tcgtttgatc ggtctcatgg atataagacc acgcttgatg ctggttattt gtatccgatt 121 tttgttgacg aagccctgcc gggtgatacg ttcaatttgc gtctgacgac gtttgctcgt 181 ttggcgactc caattaagcc gtttatggat aatcttcatg ccgatgtgtt cttttttgcg 241 gtaccctatc gtcttgtgtg ggataatttt aagaagtttt ttggagagca gaacaatccg 301 gctgactcca cgtcttttct tgtgcctcag tttacggctc ataagcctgt taccggttcg 361 ctcagtgatt atatgggctt gcctgttgat ggtacctctc ctgttttggc tggtaatatt 421 acttataact cgctccatca tagggcttat aacctgattt ggaatgagtg gttccgtgat 481 cagaatatgc agaattcggt gacggtggat aaaggtgacg gtcccgataa tatcgctaat 541 tatgttctct tgcgtcgtgg taagcgccac gattacttta catctgcttt gccgtggccg 601 cagaaaggcg ttgccgtttc gatcccgctt ggtacgtctg ctccggtcaa gactagttcg 661 acgcagcagt ttaatactaa tggtgcttct gcgttggtgt ttcgtgatct taataatgtg 721 ttgccaggag ccagtaatag tattggtgct tctaatgtca ctggtgctgc atttttatct 781 aataccgctg ctacagcgtc tgctcagttt ttggcgccct ctaacttgta tgccgatctt 841 tctacagcta cggcggctac tattaactcg ctgcgtcagg cgtttcagat tcagaagatt 901 tatgagcgtg atgcgcgtgg tggtacgcgt tatacggagt tgattcaatc tcattttggc 961 gttacgtctc cggatgctag gttgcagcgt cctgagtatc ttggtggcgg ttctaccgcg 1021 attaatgtga atcctattgc tcagactggt cagactggtc tgactggtgg cggtacgccg 1081 caaggtaatc ttgctgcaat gggtacgttc caccatagtg gtccgggttt ttctaagtcg 1141 tttacggagc attgtcttat tctcggtttg gtgaatattc gtgctgatct gaattatcag 1201 cagggtatga accggatgtg gacgcgtcag acgcgttttg atttttattg gccggctctt 1261 gcgcatattg gtgagcaggc gattcttaat caggagattt attgtgttgg tactcctgct 1321 caagatactg ctgtgttcgg gtatcaggag cggttcgcgg agtatcgtta taagccgtct 1381 atcattactg gtcagttccg ttcgacgttt gcgacgacgt tggatgtgtg gcatctcgcg 1441 cagaattttg cgtcgttgcc tgctttgaac tctacgttta ttgttgacaa tccgccgctt 1501 gcgcgtgttg ttgcagtgcc tacgcagccc atgttcttaa tggattgctt ttttaatctg 1561 cgttgtgccc ggccgatgcc ggtgtatggc gttcccggta atattgatcg tttctaagga 1621 gctatcatgg aaccgttgac aactgctttg attaccggtg gtgctagtct actgggtaac 1681 gtgttttcgt cggcgatgaa tgctggtcag tctcacgata atacgatggc taatattgct 1741 aatcagcaat gggctctcgg tgagtctgag cgttttaatc aggagcaggc tgatttgcag 1801 cggcagtggt ccgctagtca gatcaatcag cagcaggcgt ttcaggagcg tatgtctggg 1861 agtgctttcc agcgtgcgtc agctgatatg cagaaagctg gtatcaatcc gattatggcg 1921 ttcggtaatc ccgcttcgtc tccgtctggt gcgtctggcg gtgggcaatc cgccagtgtg 1981 tctactccgt cgatgccgca gtttacggag caatctaagt tttctggttt ttccagtttg 2041 ggcgatgcgg tgtctaaggc tatgtcgtca gctgttcagg tgaagaccat ggataagatg 2101 gcggaggaga tggctgaaat taaggcgcgt gaaacgaaag agaaagcgga gacgctttcg 2161 gaattgaagc gtccggcgct gattgaagct cagactagta cggagcataa gcgcccggct 2221 aatattgagg ctcagactgc tactgagcag aagaagcctg ctagtgttga agctcagact 2281 gctcaaacgc tgacggaaga gcgtttgcgt cgttatggtc tggaacaggc tgcgtttcag 2341 gagaagtccg cgaaggactt gaatcaaatg tcggatgagg gccgtaagac ccttaatgtc 2401 actagttggg gtatgcagaa ggttatggat atcctagctc cgttcctgtc tacggcgaga 2461 acggctaatc aaaccttaag gaggtagaag atgtctacga agtcgtttcc tcaggccagc 2521 catgatttta tcgcgtacaa ccataccgat acggcggtgt ggaaccatga gccttcgctt 2581 acccgtcagg aatttgcgga ggagtgtgat atcaacgcgc ttatggcgcg atataataat 2641 catgtaatcg gtggtcccgg taatttggct cctagtgagc cgatgtattt cgattttgcg 2701 gatatgccgc aagatcttat gggttatatg cagtttatgg aggattgtga gaagtctttc 2761 atgtccctcc ctgctattgt tcgtaaggag tttgataact ccgcgcttga gttcgttgca 2821 tttgcctctg atcctactaa tctccagcaa atgcgtgatt ggggcttggc tccacctgcg 2881 aaggtgaacc ctgcccctgg ggcggccgct gatgcgtcta attcctctcc gccttcggcc 2941 gccccgcccc cttctcagag cgcgcctagc gcgtcctgag acccgccagc acatatgtct 3001 acttgataca tatgtgctaa gtgacagcca acattggtcc ggaggaccta tcatgaagcg 3061 acataagatg agttctagtc attctaagcg tagtttctcg aaaggtgcga gttatacgca 3121 taagaagaat atgccgtctc gtttgcccat gcgtggtggt attcgtctgt gagttgtgtg 3181 aaaccgctca ctgcgttctg gacttcccgc tcgcgggagt ccattacgtt tgacaagtca 3241 aaaagtctga ataagattgg ttttcttctg ccatgtggtc ggtgtattgg gtgtcgtatg 3301 gataaggcca atcaatgggg attgcgttgt ctccatgaga agaagatgtg gaagtggaat 3361 ttttttgcca ctctcacgta tgatgatgaa agtttgcctc ccggcggtac gttgtgtctc 3421 cgtgatgtgc agttgtttat gaagaagctt cgctttgcga agaattctac ggtagagaat 3481 ccggttcggt tttttctagc tggagagtat ggtgatgtta ataagcgtcc gcattatcat 3541 gccttgttgt ttaattgccc ctttactgat ctcgtgcgct ggagttcagg taaaagaggt 3601 gagccacttt acacgtctcg agagttgtct gaattgtggg gaaaaggtca ttgttccctc 3661 ggtgaagtca cgtacgagag tgcggtctat tgtgccaagt atggattgaa gaagttgaag 3721 gttaatgctg atagccctcc tgctattcgc gaggagtatg aaaggaggta tgtggtttat 3781 gatgctgatg gtgtcgtgta tgagagagtg ccggagtttg ctgtgatgtc gcgtcgtccg 3841 ggtatcggag ctgattatta taataagtat ggtaaggaag tgcgcgatca tgatagtgtt 3901 gttgtgagtg gtcgtaaggt tcgccctcca cggttttatg atggtcgttc tgaagttgtt 3961 gatcccgaga ggttcgagga gcttaaggcg gctcgtaagc gtgaagcggt gaaggaagtt 4021 aataaggctg ataacacgcc ggagcggctg cgtgttaagg aagagatttt gaaagccgct 4081 gagaagcgaa aagagaggaa tttgtgatga agctggaagt gtttacggtg tatgatcggc 4141 agattaatgc tcatatgcag ccgtttttct gtcgtacgaa aggtgaagcg attcgttcgt 4201 ttacggaggc tgttaatgat ccgtctaagc cctttggaaa gtattctacg gattattctc 4261 ttatgtgcct tggtgagttt gatgatggta gcggcacttt cgtgtgccat gatcctgtgc 4321 gagttatttc tgctaatgaa gtgattgaag ctacgtctcc taccgcggag gatccacccc 4381 cggttctgcg tcgtcttccc atgtagggga tgtggaaagg ggggtctttc gacccccctt 4441 ttttttatct tggtattttg attgctttta attttacgat tagtgcctcc aagtcggctt 4501 ggatcgattt gtatactggt aatagttgcg ggcttttgct actgttctgt gcccgctttt 4561 gtgattgtac tgccatttct acggcggata ctagcatgtc tcgttcttgc tctgtgaaca 4621 ttgtttaacc tttcagtagt gtgatacgtt tgcggtgtat tccgcgtggt catgtgtatc 4681 aggcatttat taataattct actgccttgt acagtttgtg tatgtatttt ttttattggt 4741 tgtcttgacg acgtttgccg aatcttggaa tcatggaggg gtgcaagggg acgaagtccc 4801 ctaagcggca ggccaaggcg tagcgcgaag cgcgtcatca cggcgattta tcgccgtgcc 4861 ctgcaggtcc cttaaccgtt ggagaaacct c //