LOCUS MH617710 4811 bp DNA circular ENV 05-AUG-2018 DEFINITION Microviridae sp. isolate ctbc653, complete genome. ACCESSION MH617710 VERSION MH617710.1 DBLINK BioProject: PRJNA393166 BioSample: SAMN09074552 Sequence Read Archive: SRR7122375 KEYWORDS ENV. SOURCE Microviridae sp. (animal metagenome) ORGANISM Microviridae sp. Viruses; ssDNA viruses; Microviridae; unclassified Microviridae. REFERENCE 1 (bases 1 to 4811) AUTHORS Tisza,M., Buck,C., Pastrana,D., Welch,N. and Peretti,A. TITLE Uncovering a Universe of Circular DNA Viruses in Animal Metagenomes JOURNAL Unpublished REFERENCE 2 (bases 1 to 4811) AUTHORS Tisza,M., Buck,C., Pastrana,D., Welch,N. and Peretti,A. TITLE Direct Submission JOURNAL Submitted (12-JUL-2018) Lab of Cellular Oncology, National Cancer Institute, 37 Convent Dr., Room 4118, Bethesda, MD 20814, United States of America COMMENT ##Genome-Assembly-Data-START## Assembly Method :: SPAdes v. 3.11.0 Genome Coverage :: 7.297028x Sequencing Technology :: Illumina ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..4811 /organism="Microviridae sp." /mol_type="genomic DNA" /isolate="ctbc653" /isolation_source="minnow tissue" /db_xref="taxon:2202644" /environmental_sample /country="USA" /collection_date="01-Jun-2017" /note="genome binned from sequencing reads available in SRX4043871; metagenomic; derived from metagenome: animal metagenome" CDS 1..1638 /inference="protein motif:CDD:222784" /codon_start=1 /transl_table=11 /product="major capsid protein" /protein_id="AXH77910.1" /translation="MMHRNQSASTHQFAMIPKAEIPRSSFRKQHTHKTSIDASRLYPI YLDEVLPGDTFACRMTAFARMSTPIFPIMDNLHLETFFFYVPCRLLWTNWVKFMGQQD NPSDSISYTIPQIVSPPSGHPPFTLYDYFGLPTSPQIGAGNTYSHSALPLRAYNKIFK DWFKDENLYTTGVSINTGDGPDPSTDYFIRRRGKRHDYFTSALPWTQKGASVTLPLGT SAPVITLPNNITNFTGNTLQMGVVGSGASVGALYTGTNSTGKMSTGSSATGGAFTAAS QIYPMNLYADLSQATASTINQLRQSFQIQKLLERDARGGTRYTEIIRSHFGVISPDAR LQRSEYLGGGSTPINISPVAQTQATQATITPLGNLAAYGTALANNHGFTQSFTEHGYI IGLAQIRADLSYQQGMRKLWSRSTRYDFYFPVFAMLGEQAILNQELYMTGAAADTAVF GYQERWAEYRYNPNLITGIFKSTTTGTLDAWHLAQNFTALPTLNETFIAENVPISRVV AAGALADNQQFLLDIFFDVKAARPMPLYSVPGLIDHF" CDS 1648..2691 /inference="protein motif:CDD:164789" /codon_start=1 /transl_table=11 /product="minor capsid protein" /protein_id="AXH77911.1" /translation="MSWLSRATGVHINPIKGQYSGFGNLLRDAAIAGGAALTGGSLGI GGLPSWGGLGSAMGLGAGAGAGAGGGAAALTEFTPAEIAGMGTAGSSAAMGMGGMATA AGAAGAGSWLGKLGGALGSSANWLANNSGWLGPLAAGGASLYGGAQANQANSAEAAKQ RAWEEHMSSTSWQRGVNDMRAAGLNPASAYTQGGASTPGGATAQQSDFITPALQTALA TKMNSAQTASIANEQFWNTERSGAGVNATNATRDKTEAETNLLKLDAENYESTLAKRV AETKQLLAMTEKTKADTKTINALRKAQVDLAAAEAAARKYGNVEARREAELWAGKIGD KLKYILAAKNVFK" CDS 2715..3203 /inference="protein motif:CDD:164781" /codon_start=1 /transl_table=11 /product="minor capsid protein" /protein_id="AXH77912.1" /translation="MDKHVCVPVRYRSAVQGEYDTWKVSRESGLECKDPSLARQDQKD EADINVIVRDFGLTGKLPLVAVPPTYEDFAHEVDDFQTAANLMLEANRSFMAMDAKVR AKFENDPAQFVEFCSNKDNLDEMRKMGLALPREPVDGGPVQVETVVGGIQATQKGGTA AA" CDS 3082..3330 /inference="similar to AA sequence:UniProtKB:X1VYR1_9ZZZZ" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AXH77908.1" /translation="MKCGKWVWRCLGSLLMADPFRSKPSSGAFKPPRRAGRLQRKPWS PERRAAWSAAQKARWADPDFRARALAARGAKPETEVSS" CDS 3612..3887 /note="No HHBLITS hits" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AXH77909.1" /translation="MRGGGTCSNELLQTVTGMEIAQRGSRHVGPPRGGAPGSDPVRAV HWVPGGPAGGVGATDCEGGADVARAREGVCDVHVQGRKTTLGFRFTP" CDS 3637..4560 /inference="protein motif:CDD:222782" /codon_start=1 /transl_table=11 /product="putative replication initiation protein" /protein_id="AXH77913.1" /translation="MSCYKPLQAWKSLSGGPVMWDPRGAGPQVAIPCGQCIGCRVDRQ EAWALRIAKEARMWPAHEKVFATFTYKEEKLPWDLGLHHEHWQKFIRALRDYEFNKAL ASSRPARAISYFMCGEYGGLFKRPHYHACIFGAVDFMDRKDMGDTWTSPTLEKIWGNG IVRCGDINMTTARYCAAYVIKRTNGKMAEEVNPETGLKRYERFHSQTGEIVEVKPEYA KMSLRPAIGKRWYERYGIDEMKKNGLVENGREWKIPKYFWEKFKETHPEDAEVLAYDR LKKGNRKERTPERLAVRERVHKAKIQFNEER" CDS 4560..4808 /inference="protein motif:CDD:222780" /codon_start=1 /transl_table=11 /product="nonstructural protein" /protein_id="AXH77914.1" /translation="MIYRVVSIRDRVADVFAQPAFVVSAGAAVRSFGDALGKDEQLGA HPEDYDLWEIGAYDDSTAELVNLPNRQLALGKDFKKGD" ORIGIN 1 atgatgcacc gaaatcagag tgcgagtacg catcagtttg cgatgattcc gaaagcggaa 61 atcccgcgta gtagttttcg caagcagcac acgcacaaga caagcattga cgcgagtcgt 121 ttatacccca tttatttaga tgaagtattg cctggtgaca cgtttgcctg tcggatgacg 181 gcgttcgctc gtatgagcac gccgattttc ccgatcatgg acaacctgca cctggaaacg 241 tttttctttt acgttccctg caggctgcta tggaccaatt gggttaagtt catgggccag 301 caggataacc cctcggatag cattagttat acaatccccc agattgtaag cccaccgagt 361 gggcatccgc cgtttaccct ttacgattat tttgggttac cgaccagtcc gcagattggc 421 gcaggcaata cctatagcca cagcgcgcta ccgttgcgcg catacaacaa gatattcaaa 481 gattggttta aagatgaaaa cttgtataca acaggcgttt cgattaacac cggtgatggc 541 ccggacccgt caacggacta ttttatacgt cgacggggca agcggcatga ttactttacg 601 tcggctctgc cctggactca gaagggcgct agcgtcaccc tcccactagg tacgtccgcc 661 cccgtcatta cattgccgaa caacatcacc aacttcactg gcaatacgtt gcagatgggt 721 gttgtgggtt cgggtgcgtc ggtgggtgcg ctgtacacgg gtacgaattc caccggaaaa 781 atgagtaccg gcagttcagc gaccggcggc gctttcaccg ctgcttctca gatttatccg 841 atgaaccttt atgccgatct ttcccaggca acggcttcaa ccattaacca gttgcgccag 901 agtttccaga tccaaaagtt gctcgaaaga gatgcgaggg gcggaacgcg gtacacagag 961 attatccgga gtcattttgg cgttatatca ccggatgcca ggctacagcg tagcgagtat 1021 ctcggcggcg gatccactcc gattaatatc agccctgttg cgcagacaca ggcgacgcag 1081 gccactatca caccactggg taatttggcg gcctatggca ccgccttggc aaacaatcat 1141 ggatttacgc agagctttac agagcatggg tatattatcg gcttggcaca gattagagcc 1201 gaccttagtt atcaacaggg tatgcgtaag ctgtggagtc gttcgacaag gtacgatttt 1261 tacttcccag tgtttgcgat gttgggtgag caagctattt tgaatcaaga actctatatg 1321 accggagcgg cagccgacac cgccgttttc ggctaccaag agagatgggc agaatatcga 1381 tataacccaa acctgattac aggcattttc aagagcacga caacaggtac attagatgcg 1441 tggcacttgg cccagaactt tacggcactg ccgacgctaa atgaaacctt tattgccgag 1501 aatgtaccga tttcccgagt cgttgcagcc ggagcgttag cagacaacca gcaattcctg 1561 ctggatatat tctttgatgt gaaagccgcg cggccaatgc cgctctacag tgtcccgggc 1621 ctcattgacc acttctaagg aggcgttatg agttggttat cacgagcaac gggtgtgcat 1681 atcaacccga tcaaaggtca gtacagcggg ttcggaaacc tactcaggga tgcggcgatt 1741 gccgggggag ccgccctaac gggcggctcc cttgggattg gcgggctacc gagttggggc 1801 ggcttaggca gtgctatggg gttaggcgcc ggagcaggag ccggtgcggg cggcggagcg 1861 gcagcgttga ccgagttcac gccggcggaa attgcaggga tgggcacggc cggaagttcg 1921 gcggcgatgg gtatgggcgg catggcgact gctgcaggag cggccggggc aggcagttgg 1981 ctaggcaaat tgggtggagc cttaggcagt tcagcgaatt ggcttgccaa caatagcgga 2041 tggctcgggc ccttagccgc cggtggagcc agcctttacg gcggagcgca ggcgaatcaa 2101 gccaattccg cagaagcggc gaagcagcgc gcatgggaag agcatatgag ctcgaccagt 2161 tggcagcgag gcgtgaatga tatgagggcg gcgggcttaa acccggcgag cgcctacacg 2221 caaggcggag ccagtacacc aggtggagca actgcgcagc agtcagattt tattaccccg 2281 gcattacaga cagcgttagc gactaagatg aacagtgcac agaccgcgag cattgcaaac 2341 gagcagttct ggaatacgga gcgaagtgga gcgggcgtga atgcgacgaa tgcgacacga 2401 gataagaccg aggcggagac caaccttttg aagttggatg cggagaatta cgaatcgacg 2461 ttggccaaac gagtcgcgga gacgaagcag cttttggcga tgacagagaa aaccaaggcc 2521 gataccaaga ccatcaatgc gttgaggaaa gcccaagtcg acctggcggc agccgaagcc 2581 gcagcaagaa agtatgggaa tgtagaagcg agaagagaag cggagctttg ggccgggaag 2641 attggggata aattgaagta cattttggca gccaaaaacg ttttcaaata gctatatata 2701 aggagcggat ttatatggac aagcatgttt gtgtgccggt acgataccga tcagcagtac 2761 agggcgagta cgatacatgg aaggtgagca gagagagtgg gttggaatgt aaggacccga 2821 gcctagcgag gcaggaccag aaggacgagg cagacattaa cgtgattgtg agagattttg 2881 gtttgacagg aaagttaccg ttagtcgccg tgcctcccac ctacgaagat tttgcacacg 2941 aagtggacga tttccagaca gcggcgaatt tgatgctgga agcgaatcgc agcttcatgg 3001 cgatggacgc gaaggtacgg gccaagtttg agaacgatcc cgcacagttc gtcgagtttt 3061 gcagcaacaa ggacaactta gatgaaatgc ggaaaatggg tttggcgttg cctcgggagc 3121 ctgttgatgg cggacccgtt caggtcgaaa ccgtcgtcgg gggcattcaa gccacccaga 3181 agggcgggac ggctgcagcg taagccttgg agcccagagc ggcgggccgc gtggtccgcc 3241 gctcagaagg ctcgatgggc ggacccggat tttagggcgc gggcgttagc cgcgcgaggg 3301 gcgaagccgg agaccgaggt ctccagctag aacagtacat atcgagcata ggtactgttc 3361 tagggtgttt taacgaaaac aggaggttag catgcgtagg cattcagtca acaaggggcg 3421 aggcgcccgg gcgttttcca gagacgtggc cacgaccaag cggatcaacg tggcgccgcc 3481 cccgatgcgg ggcggatatc ggatgtagcg cgctatgagg gcgctagaga cggtggcgtg 3541 ggtatgggtc gccggacggg ttgggcggtg gattctcgcc gctgcgggcg ctacagagct 3601 tctgggcgag tatgaggggt ggtggcacct gctcaaatga gctgctacaa accgttacag 3661 gcatggaaat cgctcagcgg gggtcccgtc atgtgggacc cccgcggggc ggggccccag 3721 gtagcgatcc cgtgcgggca gtgcattggg tgccgggtgg accggcagga ggcgtgggcg 3781 ctacggattg cgaaggaggc gcggatgtgg cccgcgcacg agaaggtgtt tgcgacgttc 3841 acgtacaagg aagaaaaact accttgggat ttaggtttac accatgagca ttggcagaaa 3901 tttatcagag cattaagaga ctacgaattt aacaaggcac tggcgagtag ccgccctgca 3961 cgcgccatca gttatttcat gtgtggggag tacggcggct tgtttaagag accgcattac 4021 cacgcttgta tatttggcgc ggtagatttt atggaccgga aggatatggg tgacacatgg 4081 acgagtccaa cattagaaaa gatatgggga aacggcatag tgaggtgcgg cgacatcaac 4141 atgacaacag cacgttattg cgccgcctac gtgatcaaaa ggacaaacgg aaagatggcg 4201 gaagaggtca atcccgagac tggattgaag agatacgaga gattccacag ccagacgggc 4261 gaaatcgtgg aagtgaagcc agagtatgca aagatgagtt taagaccagc tattggaaag 4321 agatggtacg aaagatatgg aatagatgag atgaagaaga atggattagt agagaatggg 4381 agagaatgga agataccaaa gtatttttgg gagaagttta aagagacgca tccagaagat 4441 gcagaggtat tggcgtatga cagacttaag aaagggaata ggaaggagcg tacacctgag 4501 agattggcag ttagggaacg agtacataaa gcgaagatac aatttaacga ggagcgataa 4561 tgatttaccg tgtagtttct attcgggaca gagtagccga tgtgtttgca caaccggcct 4621 tcgttgtcag tgcgggcgca gccgtgaggt cctttggtga cgctttagga aaagatgagc 4681 aattgggtgc gcacccggaa gattatgatt tgtgggagat tggagcgtac gacgatagta 4741 ccgccgagtt ggtcaacctg ccgaatagac agttggcctt ggggaaagat tttaagaaag 4801 gagattaata c //