LOCUS MH649032 4299 bp DNA circular ENV 29-MAR-2019 DEFINITION Microviridae sp. isolate ctbf007, complete genome. ACCESSION MH649032 VERSION MH649032.2 DBLINK BioProject: PRJNA393166 BioSample: SAMN09074539 Sequence Read Archive: SRR7122366 KEYWORDS ENV. SOURCE Microviridae sp. (animal metagenome) ORGANISM Microviridae sp. Viruses; Microviridae; unclassified Microviridae. REFERENCE 1 (bases 1 to 4299) AUTHORS Tisza,M., Buck,C., Pastrana,D., Welch,N. and Peretti,A. TITLE Uncovering a Universe of Circular DNA Viruses in Animal Metagenomes JOURNAL Unpublished REFERENCE 2 (bases 1 to 4299) AUTHORS Tisza,M., Buck,C., Pastrana,D., Welch,N. and Peretti,A. TITLE Direct Submission JOURNAL Submitted (19-JUL-2018) Lab of Cellular Oncology, National Cancer Institute, 37 Convent Dr., Room 4118, Bethesda, MD 20814, USA COMMENT On Mar 29, 2019 this sequence version replaced MH649032.1. ##Genome-Assembly-Data-START## Assembly Method :: SPAdes v. 3.11.0 Genome Coverage :: 13.47x Sequencing Technology :: Illumina ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..4299 /organism="Microviridae sp." /mol_type="genomic DNA" /isolate="ctbf007" /isolation_source="red snapper tissue" /db_xref="taxon:2202644" /environmental_sample /country="USA" /collection_date="01-Jun-2017" /metagenome_source="animal metagenome" /note="genome binned from sequencing reads available in SRX4043880; metagenomic" CDS 1..1614 /inference="protein motif:CDD:222784" /codon_start=1 /transl_table=11 /product="major capsid protein" /protein_id="AXQ66006.1" /translation="MAHKSNMTHSFSNVPTADIPRSVFNRSHVHKTTFNAGDLIPIYV DEALPGDTFKLNAALLARMQTPIVPIMDNLFLETFFFAVPNRLLWDNWEKMNGARDNP TDSVDYLVPQIPIQNQPIGSMSDYFGLQIQKAGEYKVSALPFRAYNLIYNEWFRDQNI QNSEPINTGDSDDVISDYHVMKRCKRPDYFTSCLPWPQKGADVMLPLGDTAPVIGDST SLGLMDGTNALGLCSAPSGTSLTGRQTYETSTIGDVVSTGGNPVNARVLGVSDLASQS GLIADLSDATAATINNIREAFQVQRMLEKDARGGTRYVELLKQHFKVVSPDFRLQRPE YLGGGSQRISFSSVPQTSETDTTEQGRLGAFALGASPRNGFNKSFVEHCTIIGIANIR ADITYQQGVNRMWHRRTRYDFYWPVFAHLGEQEVLNKEIYMGVDPDDDKTFGYQERWS EYRYKPSIITGDFRSDSNVSLDVWHLAQDFGSRPALNDAFISDATGTVLNRTVYFPSE YQFKMDMFFDLVSVRPMPTYSVPGLVDHF" CDS 1614..2366 /inference="protein motif:CDD:164789" /codon_start=1 /transl_table=11 /product="DNA pilot protein" /protein_id="AXQ66007.1" /translation="MAWPAVAAAGLQIGSSLLSSALNVSEGRKNRRFQRRMSNTAHQR EVADLKKAGMNPTLTTGGGGSTTPSGSIIRVENPAAGAISSALDYKRTNIEKKYKGQA GSLAEESANTQRIQQGLYGAQQLKTQADTYNTLQSILESNSRVKLNAANERMILEGKL PGEKFKGSVYTKTSKGVSNLQKLFNDVRNSVSDPNTNMLEGLRLQQQKNRLDKEAARR LKLKRFKEGWKKTKGKYHWKKMKSKRNDPLHK" CDS 2560..3492 /inference="protein motif:CDD:222782" /codon_start=1 /transl_table=11 /product="replication initiator protein" /protein_id="AXQ66003.1" /translation="MTCYHPLVGYRSRAGRNPNGRWPLVFNTSEGYIDLPVTMKCGKC IGCRLAHSKEWAVRCVHEASLHTNNCFVTLTYRDEALPEYGSLNKRHFVLFMKSLRKK FGAGIRFFHCGEYGEKLRRPHHHACIFNHQFEDKKLWTVRRGVKLYRSPSLEKIWKHG YSSIGDVNFQTAAYCARYIMKKINGPLALEHYNTFDKTTGEIFKELVPEYITMSRCPG IAKEWFKKWSSDVYPSDMVKLFNQVPCKPPRYYDNLYDIENPVQMKIIKQRRLKKFNS ENNTSQRLKARKTVLQLKTKSLKRSYEQEKNSNE" CDS 3464..3754 /inference="protein motif:CDD:222780" /codon_start=1 /transl_table=11 /product="nonstructural protein" /protein_id="AXQ66004.1" /translation="MSRRKIQMNNSNNLYTIRDSKCGSYNQPFPSQHDISAKRQVSMV VNAGGTLLSEFPGEYELYKCGEFDADTGKVKTIELEHICCLSSLKTSKTEEK" CDS 3754..4191 /inference="protein motif:CDD:164781" /codon_start=1 /transl_table=11 /product="internal scaffolding protein" /protein_id="AXQ66005.1" /translation="MLKSPKRLIDRKKSQTNTGSESLVQQNQAAACNINTMMKKYQST GVLNTMQKQAQYGDFSTANDFREAKQKVLDAEENFNKLPSHIRERFNHSPFFLLDFML KEENREEAIELGFIEEPMPLEEQIVPVNDNDKLSEKAATEGTE" ORIGIN 1 atggcacata aatcaaatat gacacactcg tttagtaatg ttccaactgc ggacattccg 61 aggagtgtat ttaatcgttc tcatgtacat aaaacaacgt tcaacgctgg agacctaatc 121 cctatctatg tcgatgaagc ccttcctggg gacacattca aactgaatgc ggccttgctt 181 gctcgcatgc aaaccccgat tgtgccgatc atggataacc tttttctgga aaccttcttt 241 tttgcagtgc ccaacagact tttgtgggac aactgggaaa aaatgaatgg tgccagagat 301 aatcccaccg attcagttga ttatctagtt cctcaaattc cgattcaaaa ccagcctatc 361 ggctcaatgt ctgattattt cggattacag atacaaaagg cgggcgaata caaagtttca 421 gcccttccct tccgtgcgta taatttaata tataatgaat ggttccgaga ccaaaatatt 481 cagaattccg aacccatcaa tactggtgac tcggacgatg ttatcagtga ttatcacgtt 541 atgaaacgtt gtaaaagacc cgattatttt acctcttgtc ttccatggcc acaaaaagga 601 gccgatgtaa tgctccccct gggcgatact gcgcccgtga ttggtgactc aacctcactt 661 ggactaatgg acggtacgaa tgcccttggt ctttgctctg ctcctagtgg tactagtctt 721 accggacgac agacttatga gacatcaaca atcggtgatg tcgtctcaac gggtggaaat 781 cccgtcaatg ctagagtgct aggtgtttct gacctagcct cacagtcagg actcatagca 841 gacctgagtg atgcaacagc agctacaatt aacaatatca gagaagcgtt ccaagttcaa 901 cgaatgttag aaaaagatgc tcgaggtggt actcgatacg tcgagttatt aaaacaacat 961 ttcaaagtcg tttcacctga ttttagattg caacgaccag aatatctggg cggtggttct 1021 cagcgtatat catttagttc tgtccctcaa acttctgaga ctgatacaac cgagcaaggt 1081 cgcctaggcg catttgcact cggtgcctct ccccggaatg ggttcaataa atcgtttgtc 1141 gagcactgta ccatcattgg tatagcaaat attcgcgcag acataacata tcaacaaggc 1201 gttaacagga tgtggcacag acgcaccaga tatgattttt attggcctgt tttcgctcat 1261 cttggcgaac aggaagtatt aaacaaagaa atctatatgg gcgttgaccc tgatgacgat 1321 aaaacgttcg gttatcaaga acgttggtct gaatatcgct ataaaccgag tattattacg 1381 ggtgatttca ggagcgactc aaatgtatca cttgatgtat ggcatctagc ccaggacttt 1441 ggctctcgtc cagctctaaa tgatgcgttc atatcagacg caacaggaac cgtattaaat 1501 agaacagtct actttccaag cgaatatcaa ttcaaaatgg atatgttttt tgacttggtc 1561 tctgttcggc ctatgcccac gtatagcgtt cctggtttag tggatcattt ctgatggcat 1621 ggccagcagt agcagcggca gggcttcaaa ttggaagcag cctgctttcg tctgcattaa 1681 atgtaagtga gggtagaaaa aaccgtaggt ttcagcgtag gatgtcaaat actgctcatc 1741 aacgtgaagt tgctgattta aaaaaagctg gaatgaaccc tactttaaca accggaggtg 1801 gtggttctac aactcccagt ggctctatta taagagttga aaatccggct gccggagcta 1861 tcagctcagc gctagactac aaaagaacta atattgaaaa aaagtataaa ggtcaggctg 1921 gaagcctggc cgaggaaagc gcaaacacgc agaggattca acaaggattg tatggtgctc 1981 aacaattaaa aacacaagcc gacacgtaca acacacttca aagtatattg gaatcaaata 2041 gtcgtgtaaa gcttaatgcg gctaacgaac gaatgatact tgaaggaaaa ctgcctggag 2101 agaaattcaa gggcagtgta tacacaaaaa catctaaggg tgttagtaat ttgcaaaaac 2161 ttttcaatga tgttcgtaac tcagtaagcg atccaaatac gaacatgctt gaaggtttac 2221 gcttgcaaca acaaaaaaat cgactagata aggaagccgc tcgacgtttg aaattaaaac 2281 gctttaaaga gggctggaaa aaaacgaagg gtaaatacca ttggaaaaaa atgaagagta 2341 aacgaaatga tccacttcac aaataggaga aaatatgaaa tttagacgta aaatgaaaag 2401 acgttcaagt aaacgaaatt tcagtaaaaa tagtaaagtt aaatcacgca acacaatttc 2461 aactgtccgc cgtggcggga tacgtcttta aaatactgct gttagagact actatagata 2521 tatgcagaaa gatgtctcgt ctctagcagt agcaacgtta tgacttgcta tcatcccctg 2581 gtcggatatc gctctcgtgc ggggcggaat cccaacggcc gttggccgtt ggtattcaac 2641 acctccgagg gctatatcga cctccccgtc actatgaagt gcggaaaatg cattggctgc 2701 cgtctagctc attcgaagga gtgggccgtt cgttgtgttc atgaggcctc acttcataca 2761 aataattgtt ttgtaacact aacttatcgt gatgaggctt taccagagta cggctcttta 2821 aataaacgtc atttcgtttt atttatgaaa tccttacgga aaaaattcgg tgctggcatc 2881 cgattctttc attgtggcga gtatggggag aaacttagac gtccccatca ccacgcttgt 2941 atatttaatc atcaatttga ggataaaaaa ttatggacag tcagaagggg tgtaaaacta 3001 tatcgaagcc cgtcattaga aaaaatatgg aaacatggtt actcaagcat cggagatgtc 3061 aatttccaaa ccgctgccta ttgcgcacgc tacataatga aaaaaatcaa tggccctctg 3121 gccctcgaac attacaacac gttcgataaa acaactggag aaatattcaa agaattggtt 3181 ccagaatata ttacaatgag tagatgtcca ggtattgcga aggaatggtt taaaaaatgg 3241 tcaagtgatg tttatccaag cgacatggta aaacttttta atcaagtccc ttgcaagccc 3301 cctaggtact acgataatct atatgatatt gaaaaccccg ttcaaatgaa aataattaaa 3361 caaaggaggt taaaaaaatt caattcagaa aataatacat ctcaaagatt gaaggccaga 3421 aaaacagtcc ttcaattaaa aacaaaatca cttaaacgct cgtatgagca ggagaaaaat 3481 tcaaatgaat aattctaata atttatacac aatcagagat tctaaatgtg gctcgtataa 3541 tcagccgttc ccctctcagc atgatatcag tgctaaacgt caagtgtcga tggttgtaaa 3601 cgctgggggt acgctgttat cagagtttcc aggtgaatat gaactataca aatgcggtga 3661 attcgatgca gacacgggaa aagtgaaaac tattgaactt gaacacatct gttgtctctc 3721 atctcttaaa acgtctaaaa cggaggaaaa ataatgttaa aatccccaaa acgcttaatc 3781 gatagaaaaa agtctcaaac aaacacaggg agtgagtcac tagtgcaaca aaaccaggct 3841 gcagcgtgta atatcaatac aatgatgaaa aaatatcaaa gtactggagt actaaatacg 3901 atgcaaaaac aagcccaata cggcgacttc tcgaccgcaa acgactttag agaagcgaaa 3961 caaaaagttc tggatgctga agaaaacttc aataaacttc cgtcccacat ccgcgaacgc 4021 tttaaccata gcccgttttt cttactagac tttatgttga aagaagaaaa tagggaagag 4081 gcgatagagc tcggctttat agaagagcct atgcccttgg aggaacagat agttccggtt 4141 aatgacaatg acaaattgtc agaaaaggcg gctacggagg gtacagagta gacgcctgca 4201 catttcacta cttgatgtga aatgtgctaa ctgacacctt ttcttgaaaa tgggtcagta 4261 taatactata atatgttata gttaaaaaaa ggagtaaaa //