LOCUS MH616912 4759 bp DNA circular ENV 05-AUG-2018 DEFINITION Microviridae sp. isolate ctbh023, complete genome. ACCESSION MH616912 VERSION MH616912.1 DBLINK BioProject: PRJNA393166 BioSample: SAMN09074552 Sequence Read Archive: SRR7122375 KEYWORDS ENV. SOURCE Microviridae sp. (animal metagenome) ORGANISM Microviridae sp. Viruses; ssDNA viruses; Microviridae; unclassified Microviridae. REFERENCE 1 (bases 1 to 4759) AUTHORS Tisza,M., Buck,C., Pastrana,D., Welch,N. and Peretti,A. TITLE Uncovering a Universe of Circular DNA Viruses in Animal Metagenomes JOURNAL Unpublished REFERENCE 2 (bases 1 to 4759) AUTHORS Tisza,M., Buck,C., Pastrana,D., Welch,N. and Peretti,A. TITLE Direct Submission JOURNAL Submitted (12-JUL-2018) Lab of Cellular Oncology, National Cancer Institute, 37 Convent Dr., Room 4118, Bethesda, MD 20814, USA COMMENT ##Genome-Assembly-Data-START## Assembly Method :: SPAdes v. 3.11.0 Genome Coverage :: 7.514604x Sequencing Technology :: Illumina ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..4759 /organism="Microviridae sp." /mol_type="genomic DNA" /isolate="ctbh023" /isolation_source="minnow tissue" /db_xref="taxon:2202644" /environmental_sample /country="USA" /collection_date="01-Jun-2017" /note="genome binned from sequencing reads available in SRX4043871; metagenomic; derived from metagenome: animal metagenome" CDS 1..1632 /inference="protein motif:CDD:222784" /codon_start=1 /transl_table=11 /product="major capsid protein" /protein_id="AXH74280.1" /translation="MFRNKSVDVHRFSMIPKAEIPRSTFLIEKAHKTTFDSGYLVPVY CEEVLPGDTFKVSATMFARLATPLFPVMDNLHMDSFFFFVPNRLVWSNWKKFMGEQAN PADSISFLIPQQVSPVGGYAVGSLQDYFGLPTAGQVAGGNTVSHSALPVRAYNLIFNE WFRDENLQNSVTVDLGDGPDATSSVNYVLLRRGKRHDYFTSALPWPQKGGTSVTLPLG TTATVKTNASALFTGTQPSLLLKHIDGTLPGARVLLNLDPSGEGFAAGAGSAGGTKFY PANLYADLSTATAATINQLRQAFQIQKLLERDARGGTRYTEIVRSHFGVASPDSRLQR PEYLGGGSVPLIVNPVAQSSGTGITGGTTPLGTLGGLGTVVGRNGFSQSFTEHGHIIG LVSVRADLSYQQGLRRFWSRSTRYDFYFPAFAMLGEQSILNKEIYCDGSANDTAVFGY QERWSEYRYSPSQITGLFRSTSAGTIDPWHLAQKFTALPTLNTTFIQDTPPVSRVVAV GAAANGQQLIFDSVFSMQCTRPLPMYSVPGLIDHF" CDS 1791..2612 /inference="protein motif:CDD:164789" /codon_start=1 /transl_table=11 /product="minor capsid protein" /protein_id="AXH74281.1" /translation="MTFPILGAMAAAMPVVGDLINYRSQSNTNAASAHEAAVNRDFEE RMSNTSYQRGVADMQAAGLNPMLAYSQGGASTPSGNMASFTAPKMGSGFSEAAQNYQN LMLSSAQTKREETQSDLNAALSTKAGAEARVSNASAAQLEGEMVPQDTDDSKLPPGHT QSIFKSRAMEANLRALLTGDEEAFASKYFRSRADKAHLEYLAAQYGPGRAAQEVRKLK AEAEGEELSLPRKRAVAGVMRGLVPYGRGAGDLFSTAWDYADRADKAIGDFFRRK" CDS 2521..3078 /inference="protein motif:CDD:164781" /codon_start=1 /transl_table=11 /product="minor capsid protein" /protein_id="AXH74282.1" /translation="MVVELVTCFLRLGTMPIGPIRPSVIFSGGSKMLFAKINPFVRSA YNYDMDEASDASGLHCADPSLAVQSAAEEVDINTIVRRFGLTGQLPEDVRAPTYGDFT GIGNYHQAMNAIALANESFEEMPADVRARFGNDPSAFVDFCSDEKNRDEMEKLGLLVP AREAVAAVSEVLGTQVASEVKTPPA" CDS complement(3441..4031) /note="No HHBLITS hits" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AXH74279.1" /translation="MWEYVRLITCKPCCTNPRFKRHVSELRLQVKHLTGIGIHAPVMI VSNCTANLLESVASHVSRTLRSELSQFAIPMRPKTLKESTSVYVMTQSSTPVALTVTT ERVSENTSVIMRPTKLTAILAANKVSNTTHLPTVATKKGPEVAVIQRTPGRNMSVIVR QRDEAVFFMQARLVHNPHGPLTRPLHPAAHTAATRK" CDS 3474..4244 /inference="protein motif:CDD:222782" /codon_start=1 /transl_table=11 /product="putative replication initiation protein" /protein_id="AXH74283.1" /translation="MERSRQWSVRIMHEASLHEENCFITLTYDDAHIPARGSLDYSDF RAFLRRYRREVGRVRYFVCGEYGGQLGRPHYHAGIFGYAFRGDRKGHRRTALGHYVYR SALLERLWPHGNCELAELTAQSAAYMARYTFKKVCGAVADDHYRRVDSDTGEVFYLQP EFAHMSLKPGIGATWFARYQSDVFPHDRVVIKGVVGRPPRYYDQLLKRVDPDLLENVQ QKRILDNVDKWPDCTPERLAVREAVVNARVVHMKRNLK" CDS 4241..4510 /inference="protein motif:CDD:222780" /codon_start=1 /transl_table=11 /product="nonstructural protein" /protein_id="AXH74284.1" /translation="MSVKLVVALYDVKAELFHNPFTVPALGVAYRNLADQVAMVGDKE NVLSQHPGDFELWKLGVFDDETGFIQGEKQPAKVCVLSDLVVASE" ORIGIN 1 atgttccgta ataagtccgt tgacgttcac cgtttctcga tgattccgaa ggccgagatt 61 cctcggtcaa cgtttctgat tgagaaggct cacaaaacca cgtttgattc cggttacctg 121 gtgccggtgt attgtgagga agttcttcct ggcgacacct ttaaagtcag cgcgacgatg 181 ttcgcgcggc ttgcaactcc tcttttcccc gtgatggata atctccacat ggactctttt 241 ttcttttttg tccctaatcg tctcgtgtgg agtaactgga aaaagttcat gggcgagcag 301 gcgaatcccg ccgactctat ttcttttctc attcctcaac aggtttctcc tgtaggcggg 361 tatgccgttg gctcgctcca ggattacttt gggttgccta ctgccggcca ggtggccggc 421 ggcaatacgg tgtctcatag tgcgcttccg gttcgtgctt acaacctgat tttcaatgag 481 tggtttcgtg atgagaatct ccagaattcg gttacggtgg atcttggcga cggtcctgac 541 gctacttctt ctgttaacta tgtcctcttg cgtcgtggta agcgacacga ctatttcacc 601 tcggccttgc cctggccgca gaagggcggt acgtcggtaa ctttgccgct gggtacgacg 661 gctacggtta agacgaatgc gagcgcgctg tttacgggta ctcagccgtc gctgttgctt 721 aagcatatcg acgggacgct gcctggtgcg cgtgtgctct tgaatttgga tccctctggt 781 gagggtttcg cggccggagc tggttcggcc ggtggcacga agttctaccc ggcgaacctg 841 tatgctgatt tgtctactgc tacggcggcg acgattaatc agcttcgtca ggcgtttcag 901 attcagaagt tgcttgagcg cgatgcgcga ggtggtacgc ggtacaccga aatcgtgcgt 961 tcgcatttcg gtgttgcttc tccggattcg cgtttgcagc gtccggagta tttaggcggc 1021 ggatctgtgc cgcttattgt taatccggtt gcgcagagtt ccggcactgg tattacgggc 1081 ggcacgacgc cgcttggtac tttgggaggt cttggaaccg tagttggtcg taacggtttc 1141 agtcagtcgt tcacggagca cggccatatt attggcctgg tgagtgtgcg tgcggatcta 1201 tcgtatcagc aaggtttgcg tcgtttttgg tcgcggtcga cgcggtacga cttttacttc 1261 cctgcgtttg cgatgttagg tgagcagtct attctcaata aggagattta ctgcgatggg 1321 tcggccaacg atacggctgt atttgggtac caggagaggt ggagcgagta ccgttattct 1381 ccctctcaga ttactggttt gttccgttct acttctgccg gtacgattga tccttggcac 1441 ttggcgcaga agtttacggc tcttccgacg ctgaatacga cttttattca ggacacgcct 1501 cccgtgtctc gtgttgttgc tgttggtgct gctgccaacg ggcagcagct tatttttgat 1561 tctgtttttt ctatgcaatg tactcggccg ttgccgatgt actctgttcc tggtcttata 1621 gaccatttct aactgtgtta agggcccgac gtcttttctc gggcccgttt ttgagaggtt 1681 tgatctctta tgttcaaatt tctgttgctg tttcttgttg tgtttttgtt tttgtatgtt 1741 ccttatttgg ttgtcatgac ctttttacgt agcttgcgga ggttgtagtc atgacttttc 1801 caattttagg tgctatggct gctgccatgc ctgttgttgg tgatcttatt aactatcgtt 1861 ctcagagtaa tactaatgcc gcctcggctc atgaggcggc tgtgaatcgc gactttgagg 1921 agcggatgtc taatacgtcg tatcagcgtg gcgttgcgga tatgcaggcc gctggtttga 1981 atcctatgct tgcgtactcg caaggcggtg cctctactcc ttccgggaat atggcgtctt 2041 tcactgcgcc gaagatgggt tctggcttca gtgaagctgc gcagaactac cagaatctta 2101 tgctgtcgtc tgcgcagaca aagcgagaag agacgcagtc ggatctgaat gctgctttgt 2161 cgacgaaggc tggagcggag gcccgcgtgt ctaatgcctc tgcggctcag ctggagggcg 2221 agatggttcc gcaggatact gatgactcga agttgccgcc tggtcatacg cagtcgattt 2281 ttaagagtcg ggcgatggaa gctaatttgc gtgcgttgtt gactggtgat gaggaagctt 2341 ttgcgtctaa gtattttcgg tcgcgcgccg ataaggcgca tctggagtat ctggctgcac 2401 agtatgggcc aggtcgtgcc gctcaggaag tgcgtaagct gaaggcggaa gccgaaggtg 2461 aggagctgtc tttaccgcgt aagcgggctg ttgctggggt gatgaggggc ttggttccct 2521 atggtcgtgg agctggtgac ttgttttcta cggcttggga ctatgccgat cgggccgata 2581 aggccatcgg tgattttttc cggaggaagt aagatgttgt ttgcgaagat taatccgttt 2641 gtgcggtccg cttacaatta cgacatggat gaggcgtctg acgcctctgg tttgcattgc 2701 gcggaccctt cgttggctgt tcagtctgct gcagaggagg tcgatatcaa tacgatcgtc 2761 cgtcgatttg gcttgactgg tcagcttccg gaggatgttc gtgcgccgac gtatggcgat 2821 ttcaccggga tcggtaatta tcatcaggcg atgaatgcga ttgctttggc gaatgagtcg 2881 tttgaagaga tgcccgcgga tgtgcgggct cggttcggta atgacccttc ggcgtttgtg 2941 gatttctgtt cggatgagaa gaatcgggat gagatggaga agctgggtct tctggttcca 3001 gccagggagg ccgtggcggc ggtttctgaa gttttgggta ctcaggtagc ctcggaagtg 3061 aaaacgcctc ctgcgtgatt ctggcagctt ccacgcaggt ctttggcccg gctcccccgg 3121 gccttttttt ggccgttgct agcacaccta ttacttgttt atagtgtgct aggtggtacc 3181 gagatacttg tttcgaggtt ggtgtctttt aaatcaggag gttagcatgc ggcctttgaa 3241 gcgtcatggt gtgaacaaac atcgttcagc gcgtcgtttc cggcatcagg ccgggcggtc 3301 taaggcgccg aacgtggctg gagcgccaat gcgtggtggt tggagacttt gatttggcgt 3361 gttaccaccc gataccggct tggaggttag agtccggtga agtggttttt gtcgagcgcg 3421 gcgcggtcgc gcagtctctt ttacttccgt gtggccgctg tgtgggctgc cggatggagc 3481 ggtctcgtca gtggtccgtg cggattatgc acgaggcgag cttgcatgaa gaaaactgct 3541 tcatcacgtt gacgtacgat gacgctcata ttccggcccg gggttcgttg gattacagcg 3601 acttccgggc ctttcttcgt cgctaccgtc gggaggtggg tcgtgttaga tactttgttt 3661 gcggcgagta tggcggtcag cttggtcggc ctcattatca cgctggtatt ttcggatacg 3721 cgttccgtgg tgaccgtaag ggccaccggc gtactgcttt gggtcattac gtatacagaa 3781 gtgctctcct tgagcgtctt tggcctcatg ggaattgcga attggctgag ctcactgcgc 3841 agagtgcggc ttacatggct cgctacactt tcaagaaggt ttgcggtgca gttgctgacg 3901 atcattacag gcgcgtggat tccgataccg gtgaggtgtt ttacctgcaa ccggagttcg 3961 ctcacatgtc gcttaaacct gggattggtg caacatggtt tgcacgttat cagtcggacg 4021 tattcccaca tgatcgtgtt gtcataaaag gtgttgttgg tcgaccgccc cggtattacg 4081 accagttgct taagcgtgta gatcccgatc tcctcgagaa cgtgcagcag aagcgtattt 4141 tggacaacgt ggataagtgg cctgactgta cgcctgagcg tttagcggtt cgcgaagcgg 4201 tcgtgaatgc gcgagtagtt catatgaaga ggaatctaaa atgagcgtta agctcgtggt 4261 ggcgttgtat gacgtgaagg cggagctgtt ccataatccg ttcactgtgc cggctctcgg 4321 cgtggcgtat cggaatttgg ccgaccaggt tgctatggtc ggtgataagg agaacgtgtt 4381 gtctcagcac ccgggcgact tcgagctgtg gaagcttggt gtcttcgatg atgagacggg 4441 gttcattcag ggcgagaagc agccggcgaa ggtgtgtgtc ctatctgacc tggtggtcgc 4501 ttcggagtag gcgatagagc tgctagggcc cgctagcctg tggataactt cctggaaagc 4561 tggttttcgg accctacggt gatttgtaac gcgcgcggct cgtgcgcgag cttagtgact 4621 cgccccagaa gggcgagtgc atttggcgcc ggggcccgca aggccgaagt ttccaccgaa 4681 ggggaaactg gaggccttga aacgggcccc ggcgccaggt tttgggcgtg tttggttcgt 4741 ttccctcagg gaggtcccg //