LOCUS MH552567 5083 bp DNA circular ENV 29-MAR-2019 DEFINITION Microviridae sp. isolate ctbj30, complete genome. ACCESSION MH552567 VERSION MH552567.2 DBLINK BioProject: PRJNA393166 BioSample: SAMN09074562 Sequence Read Archive: SRR7122355 KEYWORDS ENV. SOURCE Microviridae sp. (animal metagenome) ORGANISM Microviridae sp. Viruses; Microviridae; unclassified Microviridae. REFERENCE 1 (bases 1 to 5083) AUTHORS Tisza,M., Buck,C., Pastrana,D., Welch,N. and Peretti,A. TITLE Uncovering a Universe of Circular DNA Viruses in Animal Metagenomes JOURNAL Unpublished REFERENCE 2 (bases 1 to 5083) AUTHORS Tisza,M., Buck,C., Pastrana,D., Welch,N. and Peretti,A. TITLE Direct Submission JOURNAL Submitted (28-JUN-2018) Lab of Cellular Oncology, National Cancer Institute, 37 Convent Dr., Room 4118, Bethesda, MD 20814, USA COMMENT On Mar 29, 2019 this sequence version replaced MH552567.1. ##Genome-Assembly-Data-START## Assembly Method :: SPAdes v. 3.11.0 Genome Coverage :: 18.191226x Sequencing Technology :: Illumina ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..5083 /organism="Microviridae sp." /mol_type="genomic DNA" /isolate="ctbj30" /isolation_source="macaque stool" /db_xref="taxon:2202644" /environmental_sample /country="USA" /collection_date="01-Jun-2017" /metagenome_source="animal metagenome" /note="closest relative: Viruses; ssDNA viruses; Microviridae; Gokushovirinae; unclassified Gokushovirinae gi|971763757|ref|YP_009216768.1|-putative-major-capsid- protein-[Gokushovirinae-GNX3R]; genome binned from sequencing reads available in SRX4043891; metagenomic" CDS join(4590..5083,1..4) /inference="protein motif:CDD:164781" /codon_start=1 /transl_table=11 /product="internal scaffolding protein" /protein_id="AXF52763.1" /translation="MSSNRLVDFMKRTQYSAMEHPAANSGTREHVTYRARYDKNGHME LEESGKVDTYAEIQSHKDSCDIHFILARYANGDLEALNKRPGVYFDATELPKTFADVL NLVADAKLTFDGLPAEVRSQFNGSYEQFLAQAGSESWLRALGYVQESAVENPLDVVEK KEVAE" CDS 1..1683 /inference="protein motif:CDD:222784" /codon_start=1 /transl_table=11 /product="major capsid protein" /protein_id="AXF52764.1" /translation="MNRNQESHFSLNPTRLDIQRSRFDRSHSVKTTFNVGEIVPFYVD EVLPGDTFSVDTSKVIRMQTLITPIMDNIYLDTYYFFVPNRLTWEHWRELNGENTSSP WTQTVEYTVPQLTAPEGGWSVGTIMDYMGVPTGVSGLSVSALPVRAYGLICNEWFRDE NLSDPLSVPIDDTTVVGSNGSTFVTDVAKGGKPYIAAKYHDYFTSCLPSPQKGPDVLI PGGTGANVPVYALSSVVPSMDNTYALFGKSVPSGGGTASTVYLRSGSSTATVVGPVRA EDASAPLDIGSGVGGFSPTNLWAVGDSSVATATINQLRLAYQIQKLYERDARGGTRYI EILKSHFGITSPDARLQRPEYLGGNRIPVNINQVTQQSATQTGSTPQGTVTGMSVTTD THSDFTKSFVEHGFVIGVMVARYDHTYQQGLERFWSRKDRFDYYWPVFANIGEQAVLN KEIYAQGTSVDDEVFGYQEAWADYRYKPNHVTGEMRSTYATSLDVWHLADKYDQLPTL SDSWIREDKSVVDRVLAVQSSVSAQLFGDIYIRNRATRPMPLYSVPGLVDHH" CDS 1361..1690 /note="No conserved domains" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AXF52759.1" /translation="MHRELLLMMRCLVIKKLGLTIVTSLIMSPVKCVVLMLLLLTFGI LLISMTSCRRFRIAGFARISLLLIVSLLSSLLFPLSFLVIFTFVTVLLVLCLFTRFLV LLTTINF" CDS 1747..2802 /inference="protein motif:CDD:164789" /codon_start=1 /transl_table=11 /product="DNA pilot protein" /protein_id="AXF52765.1" /translation="MFTEMLPVLGATTAAKGFIGAIQNSPQMQAAGRASSQIQQITQT ADKNSAFNQASADKQMDFQREANRLAMEFNSREAARNREWQERMSSTAHQREVQDLIK AGLNPVLSATGGNGASTTSGATASGVSSSGAMASADTSGNAARASLMGSYMSSMASMA NAATSALSNLTVAQRNNETSKLIAEANIANSQWTTRYTAEQQKALAHIYGRYGIQQAA TSGAYNLSAAQTSALASRFSSETALAMELTRQEGLDQRQADELAHDIWMSENYPTSSV GGLSALLNGALGNSSNPILSGVAGAIGLRNDGRGSSFGGKSRDSGRPGGASTHSYTYG SGSSGTKSSGFGGKFKK" CDS 2812..3144 /note="No conserved domains" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AXF52760.1" /translation="MMTYVMACCLCSAFMSADYLRDFPGFSFMSVVPIVWNGSGDVFS TMFHTRYGAEKSVKRLRLSGVTCSLSVVHVDDAWIVVPRAWARRIRRGLSNSFDFVKT SVRSNTFS" CDS 3291..4244 /inference="protein motif:CDD:222782" /codon_start=1 /transl_table=11 /product="replication initiator protein" /protein_id="AXF52762.1" /translation="MTVNDKVQYKVTSYQTNHVELINNIWTPTHLSFRSSLATKVVSE YIEIPCGKCLGCKLDYSSRWADRCLLEMQYHSVSCFLTLTYDDEHVPYNDNGLMTLRF TDFQKFMKRLRKCISPVKVRYFACSEYGSKSLRPHYHVIIFGWEPPDKEIIRKGPAGS WYYKSCLLERLWPFGYSLVGDATWKSCAYVARYVTKKLGSHAELEDYDLEPERIVMSR KPGLAYQWFHDHPEFVEWNSIPVTLGEDGGKSIRPPKYFEAKLEEINPELAKQRRLAR QEMAKAHKMDYAHFSTQQYLDYLKSREYNLSRRTAALRREL" ORIGIN 1 atgaatcgga atcaggaatc tcatttttcg ctgaatccta cgcgacttga tattcagcgt 61 agccgttttg accgttctca cagtgtcaaa acgactttta atgttggtga gattgttcct 121 ttctatgtgg atgaggtttt gcccggtgat accttttctg tcgatacaag taaagttatc 181 cggatgcaaa cgcttatcac gcctattatg gataacattt atcttgatac ttattatttc 241 tttgtaccaa accgtctaac ttgggagcat tggcgcgagc ttaatggaga gaatacttct 301 tctccgtgga ctcagacggt cgagtatact gttcctcagc tcacggcgcc tgaaggaggt 361 tggtctgttg gcactattat ggattatatg ggtgttccta ccggtgtgtc tggcctttcc 421 gtcagtgcgc tccctgtccg cgcgtatgga ctcatctgca atgagtggtt ccgtgatgag 481 aatctttcgg atcctcttag tgttcctatc gacgacacga ctgttgttgg ctctaacggt 541 tctacttttg ttactgatgt agcgaaaggc ggtaaacctt atattgctgc aaaataccat 601 gattatttca ctagttgcct tcctagtccg cagaaaggcc ctgatgtttt gattcctggt 661 ggaactggtg ctaacgttcc tgtttatgcg ctttcttctg tcgtcccttc tatggataat 721 acttatgctc tttttggtaa gtctgttcct tctggtggtg gtactgctag taccgtttat 781 cttaggagtg gttcttcgac ggctactgtt gtaggtccgg ttcgtgctga ggatgcttct 841 gctccgcttg atattggtag tggtgtcggt ggtttttcgc ctacgaatct ttgggctgtt 901 ggtgatagta gtgtagcaac tgctactatt aatcagcttc gcctcgctta tcagattcag 961 aagttgtatg agcgtgatgc acgcggcggt actcgttata ttgagattct taaatctcat 1021 tttggtatta cttctccgga tgcacggctt cagcgtcctg agtatctagg cggcaatcgt 1081 attcctgtta atattaacca ggttacgcag cagtctgcaa ctcagaccgg ctctactcct 1141 cagggtactg ttactggtat gtctgttact acggatactc atagtgattt tactaagtct 1201 tttgttgagc acggttttgt tattggtgtt atggttgctc gttatgacca tacttatcaa 1261 cagggtcttg agcgattttg gagccgtaaa gataggttcg attattattg gccggtcttt 1321 gctaacattg gcgagcaggc tgtcctgaat aaggagattt atgcacaggg aacttctgtt 1381 gatgatgagg tgtttggtta tcaagaagct tgggctgact atcgttacaa gcctaatcat 1441 gtcaccggtg aaatgcgtag tacttatgct acttctcttg acgtttggca tcttgctgat 1501 aagtatgacc agctgccgac gctttcggat agctggattc gcgaggataa gtctgttgtt 1561 gatcgtgtcc ttgctgtcca gtcttctgtt tccgctcagc tttttggtga tatttacatt 1621 cgtaaccgtg ctactcgtcc tatgcctctt tactcggttc ctggtcttgt tgaccaccat 1681 taacttttag aataacagta taagaggggg gctcctgccc ccctctttga aataggaggt 1741 ttttttatgt ttacggagat gttgcctgtt cttggtgcta cgacagctgc taagggtttt 1801 attggcgcga ttcagaatag tcctcagatg caggctgcag gtagagcttc gtctcagatt 1861 cagcagatta cgcagacagc tgataagaat tctgctttta accaggcttc agctgataag 1921 caaatggatt ttcagcgtga agcaaatcga ctcgctatgg agtttaattc tcgtgaggct 1981 gctcgcaatc gtgagtggca ggagcgaatg tcttctactg ctcatcagcg agaagttcag 2041 gaccttatta aggccggttt gaatcctgtt ctttccgcta ctggtggtaa tggtgcctcc 2101 acgacgtcag gagcgaccgc tagcggcgtt tcttcgtctg gtgctatggc tagtgcggat 2161 acatcaggaa acgccgccag ggcgtcgctg atgggctcat atatgtcctc tatggctagt 2221 atggcaaatg cggctacttc tgctctttct aatcttactg ttgctcagcg gaacaatgag 2281 acttctaagc tcattgctga agctaatatt gctaattctc agtggactac tcggtataca 2341 gctgaacagc agaaagctct tgctcatatc tatggtcgtt atggtataca gcaggctgct 2401 acgtccggtg cttacaattt gagcgctgct cagacttcgg cgcttgcgtc tcggttttcg 2461 tctgagactg ctcttgctat ggagcttact cgtcaggaag gtcttgacca gcgccaggct 2521 gatgagcttg ctcatgatat ctggatgagt gaaaactatc ctacctcttc tgttggaggt 2581 ttgtctgctc ttttgaacgg tgcgcttggt aattctagta atcctatcct ttccggtgtt 2641 gctggtgcta tcggtcttcg taatgacggt cgtggttcta gttttggtgg taaatctcgt 2701 gattctggcc gtcctggagg tgcttctact cattcttata cttatggttc tggttcttct 2761 ggtacgaagt cttctggttt cggcggaaaa tttaagaagt aggtgtttgt tatgatgact 2821 tatgtaatgg catgctgttt gtgctctgct tttatgtcgg ctgattattt gcgcgatttt 2881 ccaggttttt cctttatgtc cgttgttcct attgtgtgga atggttctgg agatgtcttt 2941 tcgactatgt ttcatactcg ttatggagct gagaagtctg ttaagcgtct tcgtctttct 3001 ggtgttactt gttctttgtc tgttgttcat gttgatgatg cttggattgt agtacctcgt 3061 gcctgggcgc gtcgcattcg tcgtggtttg tctaattcgt ttgattttgt taagacctcg 3121 gttcgttcca atactttctc ttgaacaacc gtttttgttg gctaccctag ttctgaggtg 3181 ttttcggcca ccccggccct atatacttga tatatagggc cggagtgaca ccttcgtcag 3241 gagtgatata tttgagctgc aataatcccc ttaaagcttt tccggtagga atgaccgtta 3301 acgataaagt tcaatataaa gttacttcat atcaaactaa tcatgttgaa ttaattaata 3361 atatttggac tcctactcat ctatcattta gaagtagtct cgctactaaa gttgtttcgg 3421 aatatattga aattccatgt ggtaaatgtc ttggttgcaa gcttgactat tcttcccgct 3481 gggctgatag atgtttgctc gagatgcagt atcattctgt cagctgcttt ctcactttga 3541 cttatgatga tgagcacgtc ccttataatg ataatggtct tatgactctg cgttttactg 3601 attttcagaa gtttatgaag cgattgcgta agtgcatctc tcctgttaag gttcgttatt 3661 ttgcttgttc tgagtacggc tctaagtctc ttcgccctca ttatcatgtg attatttttg 3721 gctgggaacc tccagataag gaaattattc gtaaaggccc tgccggtagc tggtattata 3781 agtcgtgttt gcttgagcgt ctttggcctt tcggttattc ccttgtaggt gatgctactt 3841 ggaagtcttg cgcatatgtc gcgcggtatg ttactaaaaa gcttggctct catgccgagc 3901 tggaagacta tgacctggaa cctgagcgta ttgtgatgtc tcggaagcct ggcttggctt 3961 atcagtggtt tcatgaccat cctgaatttg tggaatggaa ttctattcca gttactcttg 4021 gcgaagatgg aggaaagtct attcggcctc caaagtattt tgaggctaag cttgaggaga 4081 taaatcctga gctggctaag cagcgtcgtt tggctcgtca ggaaatggct aaggctcata 4141 agatggacta tgcgcatttt tctacacagc agtatcttga ttatttaaag tctcgtgagt 4201 ataatctaag taggcgtact gctgcgctga gacgtgaact ttagaaagga gtaattgcat 4261 gtctagaagt aaggttcgag cacggaaaga ccgcaagatt ttcaagagga ccgcgactaa 4321 gagcaaggat atcaacgttt ctccgagaaa ttttagaggg ggtattcggc tgtgatgaag 4381 catatttatt ccattcgtga ttgtaaggtt ggctatatga atcctgtcgt ggatgcgaat 4441 gatgagtacg ctattcgtaa tttcaatttt gctaaaaatg actctgagac gctgttgcat 4501 gattttcctg gtgactttgt tctttgccgg attgctgatt ttgatgacca gaccggcgtt 4561 attacgccgt atcctccgga ggtgattgca tgagctcaaa tcgtcttgtc gattttatga 4621 agcggactca gtattccgcg atggagcatc ctgctgctaa ctctggtacg cgtgagcatg 4681 taacttatcg agctagatat gataagaacg ggcatatgga gcttgaagaa agcggcaagg 4741 ttgatactta tgctgagatt cagagtcaca aggattcttg tgatattcat ttcattcttg 4801 ctcgctatgc taatggtgac cttgaggcgc tgaataagcg ccctggtgtg tattttgatg 4861 ctacagagct gcctaagacg tttgccgatg ttttaaacct tgttgcggat gcaaagctaa 4921 cttttgacgg tcttcctgct gaagttcgtt cccagtttaa cggtagctat gagcagtttc 4981 ttgcccaggc tggttctgag tcttggcttc gtgctcttgg ttatgttcag gagtctgctg 5041 tggaaaaccc tcttgatgtt gtggagaaaa aggaggttgc cga //