LOCUS MN582073 4794 bp DNA circular ENV 18-NOV-2019 DEFINITION Microviridae sp. ctbuH30, complete genome. ACCESSION MN582073 VERSION MN582073.1 DBLINK BioProject: PRJNA540674 BioSample: SAMN11552893, SAMN11552892, SAMN11552894 Sequence Read Archive: SRR8996939, SRR8996941, SRR8996944 KEYWORDS ENV. SOURCE Microviridae sp. ctbuH30 (freshwater metagenome) ORGANISM Microviridae sp. ctbuH30 Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae. REFERENCE 1 (bases 1 to 4794) AUTHORS Malki,K. and Breitbart,M. TITLE Florida's Freshwater Springs JOURNAL Unpublished REFERENCE 2 (bases 1 to 4794) AUTHORS Malki,K. and Breitbart,M. TITLE Direct Submission JOURNAL Submitted (16-OCT-2019) College of Marine Science, University of South Florida, 830 1st St S, Saint Petersburg, FL 33701, USA COMMENT Submissions were annotated with Cenote-Taker2 available at https://github.com/mtisza1/Cenote-Taker2. ##Genome-Assembly-Data-START## Assembly Method :: SPAdes v. 3.12 Genome Coverage :: 10.747393x Sequencing Technology :: Illumina ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..4794 /organism="Microviridae sp. ctbuH30" /mol_type="genomic DNA" /isolate="ctbuH30" /isolation_source="Volusia Spring" /db_xref="taxon:2656701" /environmental_sample /country="USA" /collection_date="22-May-2017" /metagenome_source="freshwater metagenome" /note="genome binned from sequencing reads available in SRX5775735,SRX5775734,SRX5775731; metagenomic" CDS 1..1587 /inference="protein motif:PHA00363" /codon_start=1 /transl_table=11 /product="major capsid protein" /protein_id="QGH72658.1" /translation="MGRPSVMGHNFSQVPQANIQRSQFNRSSGYKTTLDAGYIYPIFR DEVLPGDTFNVRANIFARLATPITPFMDNMFMDIHFFFVPMRLLWENWERFNGAQDDP GDSTDFLMPQMTAPAVTGVQVGELSDYFGIPTGIASIEFNSHFHRAYNLIYNQWYRDQ NLQNSVVVDRDDGPDTYTDYVLRRRNKRHDYFTSCLPTPQKGPAVSLPLGTEAPVKGI GKINGTWTTAQNDVYESDGTQADYWNSATNALSTIVIERQPNGSPIVDYPYIRADLSA ATAATVNDLREAFQVQRLYERDARGGTRYVELLRSHFGVISPDARLQRPEYLGGSSVP VNVHPIAQTSSTDATTPQGNLAAMGTVGNSAAGFVKSFVEHGVVLGLVSIRADLTYQQ GLERCFSRQIRWDFYWPSLAHIGEQSVLNKEIYMDGSANDDAVFGYQERFGEYRYKPS LITGKFRSTYAQSLDMWHLSQEFGSLPTLNSTFIQETPPMARVKAVTDEPDFLLDAYF NTITARPMPLFGVPGQIDRF" CDS 1660..1833 /note="No HHsearch hits" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QGH72663.1" /translation="MPKVSFTVGEFRKFLQDLPASMSASSALRAFLVARGAVNPRARI RTATLRVKKAKGV" CDS 1833..2579 /inference="similar to AA sequence:UniProtKB:A0A0C5AFV1_9VIRU" /codon_start=1 /transl_table=11 /product="putative minor capsid protein" /protein_id="QGH72659.1" /translation="MLPMLGAAAISGGMDLAGQMMTNAQNIRLAHEAQDFNRQEAEKN RDWQERMSNSAYQRATADMRAAGINPMLAYMKGGASTPGGAQASGVVARVENALGRGV SSAVAAANTAKELESKDAGIGLTNASADVARQDAKLKEVSAAKAGLEVEALASTLGAI KREGVAREKRAGVESAEADWDKDTTGVRKILDLTRSVLGVGHSAKSLMGPSLNLDIGS TPGPAKPGDKDYDKYWNTRTREKMKSQRRP" CDS 2591..3115 /inference="protein motif:PFAM:PF09675.9" /codon_start=1 /transl_table=11 /product="scaffold protein" /protein_id="QGH72660.1" /translation="MPDKRDLEELPSVIVSGAVGKKRKPGERVRVQLFCRDESRTDQQ FKKDSEITSILEKARRQGFLPGDPSRARYGDVSGFVDYQTSLETVMRGQEAFSALPAK VRARFENDPQQFVSFVMDPKNGKELVQMGLATAKVAPSGALNVQGAGTPAPKPEGAPK KAPKGESPPPKEGD" CDS complement(3169..3360) /note="No HHsearch hits" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QGH72664.1" /translation="MKKYNIELKGYEKQKLTYVLARIIHELEIRGDYPLKRQDLSILK IIFLKLTQPPVRPRKTDSK" CDS complement(3470..3787) /note="No HHsearch hits" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QGH72665.1" /translation="MHTANRPLARSLKATAYALTTSKSHRLIDSEPLTKNNTTLARSV YRLRSPPFNRVVAGQKYLQSNATAHRPREQITSVNTLSGLRENPLRLTRRHLLTLHEN LQS" CDS 3782..4543 /inference="protein motif:PFAM:PF05840.12" /codon_start=1 /transl_table=11 /product="replication associated protein" /protein_id="QGH72661.1" /translation="MHEADLYNKNCFITLTYSKRYLPPGGTLVLSHFQDFMKRLRFRF GPGIRFFHCGEYGELRGRPHYHALLFNFDFPDKYEWRRTDLDFQCWRSPALEELWRFG NSEIGSVTFESAAYVARYITKKITGPMAALAYTDFDLVTGEVFGEKKPEYCTMSRRPG IGAGYYSAFSRDIFRRSGFYIRRDDKYIKCRPPKFYDGRYEIDDVADFERVKEERGEL ARKLNTDNSPERLEVRRKVHERRARMLKRGFEDGR" CDS 4494..4793 /inference="similar to AA sequence:UniProtKB:A0A0G2UEC9_9VIRU" /note="DNA binding protein VP5" /codon_start=1 /transl_table=11 /product="DNA binding protein" /protein_id="QGH72662.1" /translation="MSVELVCLREVLKMVVKMFTVYDSKAVAFRLPMFFNSVGQAVRG FGDIARDKENEIGRHPGDFTLMQIGDFDTETGAVTMLESKINLGSALEHASQENR" ORIGIN 1 atgggtcgtc cctcagtgat gggtcataat ttttcgcagg tcccgcaagc gaatattcag 61 cgttcgcagt ttaaccgctc gtcgggttat aagactacgt tggatgcggg ttatatttat 121 cccatcttca gggacgaggt gttgcctggt gacacgttca atgttcgcgc gaacattttc 181 gctaggctcg ctaccccgat tactccgttc atggacaata tgttcatgga cattcatttt 241 ttcttcgttc cgatgcgtct cctttgggag aactgggaga ggtttaatgg agctcaggat 301 gatcctggtg attctactga tttcttgatg cctcagatga ccgctcccgc ggttactggt 361 gtgcaggttg gcgagttgtc ggattatttt ggtattccta ctggtattgc aagtattgaa 421 tttaatagtc attttcatcg tgcctataat ttgatctaca accagtggta cagggaccaa 481 aatttacaaa attctgttgt tgttgatcgt gatgatggtc ctgatacgta tactgattac 541 gtgttgaggc gtcgtaataa gcgtcatgat tattttacgt cttgtcttcc tactcctcag 601 aagggtcctg ctgtttcgtt gccgcttggt actgaggccc ctgtaaaggg tatcggtaag 661 atcaatggaa cttggactac ggctcagaat gatgtgtatg agtcggatgg tactcaggct 721 gattattgga attccgctac taatgctctt tcaacgattg ttattgagcg tcagcctaac 781 ggtagtccga tagttgatta cccttatatt cgtgccgatc ttagtgcggc cactgctgct 841 actgttaatg atttgcgtga ggcttttcag gttcagaggc tttatgagcg tgatgctcgt 901 ggtggtaccc ggtacgttga gttgttgcgt tcgcatttcg gtgtcatttc tcccgatgcg 961 cgtttgcaaa ggccagagta tttgggcggt agtagtgttc cggttaatgt tcaccccatt 1021 gcccagacaa gttcgacgga tgcgactacg ccgcagggga atcttgcggc catgggcacc 1081 gttggtaact ctgctgctgg gtttgtgaag tcttttgttg agcatggtgt tgtgcttggt 1141 cttgtttcta ttcgtgcgga tttgacttat cagcaaggtc tggagcgttg tttctctcgt 1201 cagattcgtt gggatttcta ttggccgtcg ttggctcaca ttggtgagca gtccgttctt 1261 aataaagaga tttatatgga cggtagtgcc aatgatgatg ctgttttcgg ttaccaagag 1321 aggttcggcg agtacaggta caagccgtct ttgattactg ggaagtttag gtctacttat 1381 gctcagtcgt tggatatgtg gcatctctcg caagagtttg gttctctgcc tacgttgaat 1441 tcgacgttca ttcaggaaac gccgcctatg gcgcgtgtga aggctgtgac tgatgagcct 1501 gattttctgt tggatgctta ttttaatacg attacggctc gtcctatgcc gttgtttggt 1561 gtgcctggtc agattgatcg gttttaaggc gcgcgccgtt tgagcggttc ttgtcgggcg 1621 accaaggcaa cgccctggtc gccccttttt ggaggtttta tgcccaaggt ttcttttact 1681 gttggtgagt ttcggaagtt tttgcaggat ttgcctgctt ctatgtctgc ttcgtctgct 1741 ttgcgtgctt ttttggttgc tcgtggtgct gtgaatccga gagctcggat tcgtactgct 1801 acccttcgcg ttaagaaagc gaagggtgtt tgatgctccc tatgcttggt gctgctgcca 1861 tttctggtgg tatggacttg gcaggtcaga tgatgacgaa tgctcagaac attcgtcttg 1921 ctcatgaggc tcaggatttt aatcgtcagg aggctgagaa gaatcgcgac tggcaggagc 1981 gtatgagcaa tagtgcttat cagcgtgcta cggctgatat gcgtgctgcg ggtatcaacc 2041 cgatgttggc ttatatgaaa ggtggtgctt ctactcctgg tggtgcccag gcttctggtg 2101 ttgttgctcg tgttgagaat gctttgggcc gtggtgtttc ttcggctgtg gccgcggcca 2161 atacggctaa ggagcttgag tctaaggatg ctggtattgg tctgactaat gccagtgctg 2221 atgttgctcg tcaggatgcg aagctaaagg aagtttcggc tgctaaggcc ggtttggagg 2281 ttgaggcgtt agcctctacg cttggtgcta ttaagcgtga aggtgtggcc cgcgagaagc 2341 gcgctggtgt tgaatcggct gaagccgatt gggataagga tactactggt gttcgtaaga 2401 ttcttgatct tactcgtagt gttctcggag ttggtcattc tgctaagagt ttgatgggtc 2461 cttcgttgaa tttggatatt ggttctacgc ctggtcctgc taagccaggc gataaggatt 2521 atgacaagta ttggaatacg cgtacgcgtg agaagatgaa gtctcagcgt cgtccttaag 2581 ggaggtttgt gtgcctgata agcgtgattt ggaagaattg ccgtctgtga ttgtttctgg 2641 tgctgttggt aagaagcgca agcctggcga gcgcgttcgt gttcagctgt tttgtcgtga 2701 tgagtctcgg actgatcagc agtttaagaa ggattcagag atcacttcta ttttggagaa 2761 ggctcgtcgt cagggctttc tgcctggtga tccgtctcga gctcgctacg gtgatgtttc 2821 tggtttcgtg gattaccaga cgtctcttga gactgttatg cgtggtcagg aggcgttttc 2881 ggctctgccg gctaaggttc gtgctcggtt tgagaacgat cctcagcagt ttgtttcgtt 2941 tgttatggac ccgaagaacg gcaaggagct cgttcagatg ggtcttgcga ccgctaaggt 3001 tgccccgagc ggcgcgttaa atgtgcaggg tgcaggtacc cctgcaccta agcccgaagg 3061 agcccctaaa aaggctccga agggcgagtc tccacctccg aaagaggggg attaaggggg 3121 cccgaccagt tacaacactt gttgtaactg gtcggactga cagcaaggct atttgctgtc 3181 agttttacgc ggccgcacgg gcggctgcgt taatttgaga aatatgattt tgagtattga 3241 gaggtcttgg cgtttgagcg ggtagtcccc gcggatttcg agctcgtgaa tgattcgagc 3301 taggacgtaa gtcagttttt gcttttcgta gcctttgagt tcaatattgt atttcttcat 3361 aggtcctccg ttttagctgg gccatcagcg gcccctcaca ggaaagcgcg aaggcgcggt 3421 gggggtcctg atgggttagc gcgaaacgag tggcgatgta tatacgaagt taactttgga 3481 gattttcatg aagcgtaaga agatgtcgtc tcgtaagtcg aagcgggttt tctcgaagac 3541 cgctaagcgt gttaacgctc gtaatttgct cgcgcggccg atgcgcggtg gcattcgact 3601 gtaggtattt ttgccctgct actacccggt taaagggtgg agatctaagt ctgtaaacgc 3661 ttcgggcaag cgtagtattg tttttagtca gaggctcgct atcgatgagc ctgtgactct 3721 tgcttgtggt caatgcatag gctgtcgcct tgagcgatcg cgccagtggg cgattcgctg 3781 tatgcatgag gccgatctct ataataagaa ttgttttata actctcacct attctaaaag 3841 atatttgccg ccaggtggga ctcttgttct ctctcatttc caagatttta tgaagcgtct 3901 gcggtttcgc tttggtcctg gtattcgatt ttttcactgt ggtgagtacg gtgaattgcg 3961 cgggcgtccg cattatcatg cgttgctttt taactttgat tttccggata agtatgagtg 4021 gcgtcgtact gatttagatt ttcaatgttg gcgttcgcct gctttggagg agctctggag 4081 gtttggtaat agtgagattg gtagtgttac ctttgagtct gctgcttatg ttgcccgtta 4141 tattacgaaa aagattactg gtcctatggc ggcgttggct tataccgatt ttgatttggt 4201 taccggcgag gtcttcggtg agaagaagcc ggagtattgc actatgtctc ggcgacctgg 4261 tattggtgct ggctattatt ccgctttctc tcgggatata tttaggcgga gtggttttta 4321 tatacgtcgt gatgataagt atattaagtg tcgacctccg aagttttatg atggacgtta 4381 tgagattgat gatgtagctg attttgagcg tgttaaggag gagcgtggag agttggcaag 4441 gaagcttaat actgataatt cgcctgagcg tctcgaagtg agacggaagg tgcatgagcg 4501 tagagctcgt atgcttaaga gaggttttga agatggtcgt taagatgttt acggtttatg 4561 attcgaaggc tgttgctttt aggttgccta tgttcttcaa tagtgttggc caggccgttc 4621 gtggttttgg tgatattgcc agggataagg agaatgagat tggtcgtcac cctggtgatt 4681 ttactttgat gcaaattggt gatttcgaca cggagacggg tgccgttact atgttggagt 4741 cgaagattaa tctcggcagt gctctagagc acgcctctca ggagaatcgt taag //