LOCUS MH616690 5619 bp DNA circular ENV 29-MAR-2019 DEFINITION Microviridae sp. isolate ctcg539, complete genome. ACCESSION MH616690 VERSION MH616690.2 DBLINK BioProject: PRJNA393166 BioSample: SAMN09074552 Sequence Read Archive: SRR7122375 KEYWORDS ENV. SOURCE Microviridae sp. (animal metagenome) ORGANISM Microviridae sp. Viruses; Microviridae; unclassified Microviridae. REFERENCE 1 (bases 1 to 5619) AUTHORS Tisza,M., Buck,C., Pastrana,D., Welch,N. and Peretti,A. TITLE Uncovering a Universe of Circular DNA Viruses in Animal Metagenomes JOURNAL Unpublished REFERENCE 2 (bases 1 to 5619) AUTHORS Tisza,M., Buck,C., Pastrana,D., Welch,N. and Peretti,A. TITLE Direct Submission JOURNAL Submitted (12-JUL-2018) Lab of Cellular Oncology, National Cancer Institute, 37 Convent Dr., Room 4118, Bethesda, MD 20814, USA COMMENT On Mar 29, 2019 this sequence version replaced MH616690.1. ##Genome-Assembly-Data-START## Assembly Method :: SPAdes v. 3.11.0 Genome Coverage :: 16.389037x Sequencing Technology :: Illumina ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..5619 /organism="Microviridae sp." /mol_type="genomic DNA" /isolate="ctcg539" /isolation_source="minnow tissue" /db_xref="taxon:2202644" /environmental_sample /country="USA" /collection_date="01-Jun-2017" /metagenome_source="animal metagenome" /note="genome binned from sequencing reads available in SRX4043871; metagenomic" CDS 1..1947 /inference="protein motif:CDD:222784" /codon_start=1 /transl_table=11 /product="major capsid protein" /protein_id="AXH73246.1" /translation="MEKTLGGDRLGAGGKNKVELHGYGRSTHDLGYLWRSTMSAGTLV PFMCEVGLPGDTFDIDLNAVVNTHPTIGPLFGSFKMQHDIFVAPIRLYNAVLHNNALN IGRKMSNVKLPWIELTAMELGNLEALEDIDNAQTNPSCLLAYLGIRGIGVTDEPEFPR KFNATSILMYWDIYKQYYANKQEEIGAVIHTPAKPLINTVDFIDVITGASSTTIPQVP ALAVSAPLQSGTIFEIGYTGTPPLPEQIYVNVDGLGFVSLKEISSNIAPGAGTTIICQ YNHFKFGTDSVDSWRYVNANDLQQLQPEVTTFPLTNIDNMRMALLAAGNDFSINDPGI APYRYLYEINSGRYNVLSSQEGLGIKTYNSDLFNNWISTEWIDGVDGISAITAIDTSG GSFKIDTLNISNKVYNMLNRIAVADGSYQGWLDAVYTNSRHRMVETPWYAGGLIKEVI FQEVISNAENSNAGQPLGTLAGKGRLADKHKGGKIVVKIEEPSYILGIVSLTPRVDYS QGNKWDVNLRTIDDFHKPALDQIGYQELITEQMAWWETTEVSGAWVQKSAGKQPAWIN YMTNTNVVRGNFAIKRNEMFMTLNRRYETKTNGIKDLTTYIDPAKFNNIFAQTSLDAQ NFWVQIAVDIKARRVMSAKVMPNL" CDS 1999..2346 /note="No conserved domains" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AXH73244.1" /translation="MYRYQKATTTTIKRNTGYEGETIEEKIHRIVNNKEPIKDGAPRI YSERKDGVIPAYDIRTDRFEIAVDAMDKVTKEKVASRGNSLGEKAKEGMEAEAKNETN PETGANTGDSKTN" CDS 2426..3442 /inference="protein motif:CDD:164789" /codon_start=1 /transl_table=11 /product="DNA pilot protein" /protein_id="AXH73247.1" /translation="MPSGIAQLGLQTLGTAVNTGMGLLLEKHNDKRQLRQQGKLQELQ IQGDKEMSDYNYSKQLQMWKDTNYKAQQAELEKAGLNPGLIYGMSGGGATTTGSGGGN VTGGTAPSGGREVVDMQAMGLQRGLQEAQINNINADTEKKKVETAKTSGVDTELATTQ VASISQGIKNAEVQNSILKWEDNIKRIDSNVAEQTQGDRMGQIRSLAETAASTAEMIE RDNGIAAETKNEKITQIKAESIGAVIRNELTNAQIDKTGMDIKKTATEITNMVVNMGV EWAKLSIDQQKLKLQEKLVNFNTAQSQRTYENILKGINTITGSLKPGTTINQAPKETI INNY" CDS 3442..4497 /inference="protein motif:CDD:222782" /codon_start=1 /transl_table=11 /product="replication initiator protein" /protein_id="AXH73248.1" /translation="MCLYGRRIRNKKYCKNQKNGGIIPPVLDERALHIEIACGNCIEC RQKRAREWQVRLQEDIKTNKNGIFITLTLSNESYTELSGQVSNKIIRELNTLYNIGYD NQQKNRKIKKLEEKLKGYAKDNEIAKMAIRRFVERWRKKFKKSVRHWLVTELGHNGTE NIHLHGIIWTNESSETIKEIWNYGFIWTGYDRWKGKENYVNDETINYTVKYIHKVDFK HKLYKSIVLTSAGMGANYVNSINARNNKFADKETVDNYRTRNGHKLPLTTYWKNKLYT EAEKEKLWMNKLDEQKAYVCGEEINTKNGWQEYWKAIKHHRLRNNRLGYGSDKKNWQR AWYEEERRKKLQEARMI" CDS 4614..4973 /note="No conserved domains" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AXH73242.1" /translation="MDGRRKPPEEHTPSNGSRQKKKIGNKKRNRKLADYKPPKGTKLK QMKKANAEVNVTIVTQSGNDILKTKEKQMYYLKIETEAGEALLNVGEKTYNKVKFITD ENEIQKSKKLNTSSKQQ" CDS 5099..5428 /note="No conserved domains" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AXH73243.1" /translation="MESTEQKDILNMSQNVDETLKVKSGELFTRHDIQGTPFYAIEIK DKWFLALGRYKISEDFSCMEDIETYLKTNMYEVVLDMITRVWDMSLEMKMMEKPIKKP KKVTNKS" ORIGIN 1 atggaaaaaa cattaggcgg cgacagactc ggcgcaggag gaaaaaacaa agtagaacta 61 cacgggtacg gcagaagcac acacgaccta ggatacctat ggcgcagcac aatgagcgca 121 gggacactag tcccgttcat gtgcgaagta ggcctgccgg gcgacacctt cgacattgac 181 cttaacgcag tagttaacac gcatcccaca atcggaccac tgttcggatc attcaaaatg 241 caacacgaca tatttgtagc tccaataagg ctatataacg ccgtactgca taacaacgca 301 ttaaacatag gccgtaagat gagtaacgta aaactgccat ggatagagct aacagcaatg 361 gaactgggaa acctagaagc actagaagat atagacaacg cacaaacaaa cccctcatgc 421 ttattagcat acttaggaat aagagggata ggagtaacag acgaaccaga atttcccaga 481 aaattcaacg caacaagcat attaatgtat tgggatatat acaaacaata ctatgcaaac 541 aaacaggaag aaataggtgc agtaatacac acaccggcaa agccactaat aaacacagtg 601 gacttcatag atgtaataac aggtgcatcc agcacaacaa taccacaagt accggcacta 661 gcagtatcag caccattaca atcaggaaca atattcgaaa taggatacac cggaacaccg 721 ccgttaccag aacagatata tgtaaatgta gacggacttg ggttcgtatc actaaaagaa 781 atcagcagca acatagcacc gggcgcagga acaacaatca tatgccaata caaccacttc 841 aagtttggca ctgatagcgt agactcatgg agatacgtaa acgctaacga cctgcaacaa 901 ttacaaccgg aagtaacaac attcccacta acaaacatag acaacatgag aatggcgcta 961 ctggcagcag gcaacgactt ctcaatcaac gacccgggaa tagcccctta cagatacctc 1021 tacgaaataa acagtggtag gtataacgta ctaagctcac aagaagggct gggaataaaa 1081 acatacaact ccgatctgtt caacaactgg atatccacag aatggataga cggagtagac 1141 ggaataagcg ctataacagc gatagacaca tcagggggaa gcttcaaaat agacacgctt 1201 aacattagca acaaagtgta caatatgctc aacaggatag cagtagcaga cggaagctac 1261 caaggctggc tggacgcagt atacactaac agccgccaca gaatggtgga aacaccgtgg 1321 tacgcaggag gcctaataaa agaagtgata ttccaagagg taataagcaa cgcagaaaac 1381 tctaacgcag gacaaccgct gggtacactg gcaggcaaag gaagactagc agacaaacac 1441 aaaggtggta aaatagtagt aaaaatcgaa gaacccagct acatattagg aatagtaagc 1501 ctaacaccaa gggtagacta ttcacaagga aacaaatggg acgtaaacct aagaacaata 1561 gacgacttcc acaagccagc actcgaccaa ataggatacc aggaattaat aaccgaacaa 1621 atggcctggt gggaaacaac agaagtaagc ggagcttggg tacagaaaag cgcaggtaag 1681 caacccgcat ggataaacta catgacaaac acaaacgtag taagagggaa cttcgcaata 1741 aaaagaaacg aaatgtttat gacattaaac cggaggtacg aaacaaagac aaacggtata 1801 aaagacctaa caacatacat tgacccggca aaattcaaca acatattcgc acaaacatca 1861 ctggacgcac aaaatttctg ggtacaaata gcggtagaca taaaagcccg cagagtaatg 1921 agtgcaaagg taatgccaaa cctataaaca aaatttaaaa acaagggggt gggaaaccac 1981 ccccacaaaa acaaagctat gtatagatat caaaaagcaa caacaaccac gattaaaaga 2041 aacaccggat atgaaggtga aacaatcgaa gaaaaaatcc acagaatagt aaacaacaaa 2101 gaaccgatta aagacggagc gccaagaata tactccgaaa gaaaagacgg ggtaatacca 2161 gcatacgata taagaacaga caggttcgaa atagcggtag acgcaatgga caaagtaaca 2221 aaagaaaaag tggcaagcag aggtaacagc ttaggagaaa aagctaaaga aggaatggaa 2281 gctgaagcaa aaaatgaaac aaatccggaa accggagcca atacgggcga ctctaaaaca 2341 aattagaaac ttaattaatc acaagtggta cgcacgtatt catatatatg aactatggac 2401 gtaccgcttt taaaaaagcg cgaaaatgcc aagcggaata gcacaactag gactacaaac 2461 actaggaaca gcagtaaaca cagggatggg tttactacta gaaaaacata acgataagag 2521 acagcttaga caacaaggta agctacaaga gctgcaaata caaggagaca aagaaatgtc 2581 tgactacaac tacagtaaac agctacaaat gtggaaagac acaaactaca aagctcaaca 2641 agcagagcta gaaaaagcag gactaaaccc gggactgata tacggaatga gcggaggcgg 2701 agctacaaca acaggaagcg gcggaggaaa cgtaacagga ggaacagcgc caagcggggg 2761 acgagaggta gtagatatgc aagcaatggg actacaaaga gggctacaag aagctcaaat 2821 aaacaacata aacgcagata cagaaaaaaa gaaagtggaa acagctaaaa cgagcggagt 2881 tgacacagag ctagcgacaa cacaagtggc atcaataagc caaggaatca aaaacgcaga 2941 agtacaaaac tctatattaa aatgggagga caatattaag cgtatagact caaacgtagc 3001 agaacaaaca caaggagaca gaatggggca aataagaagc ctagcagaaa cggcagcaag 3061 cacggcagag atgatagaaa gggacaacgg gatagcggca gaaacgaaaa acgaaaaaat 3121 aacccaaatc aaagcagagt caataggggc ggtaataaga aacgaactga caaacgcaca 3181 gatagacaag acgggcatgg atataaaaaa gacagctaca gaaataacaa acatggtggt 3241 aaacatggga gtagaatggg caaagctatc catagaccaa cagaaactaa agctacaaga 3301 aaaactggta aacttcaata cagcacaatc gcaaagaacg tatgaaaaca tactaaaagg 3361 cataaacaca ataacaggct cactaaaacc gggaacaaca ataaaccaag cgccaaaaga 3421 aacaataata aacaattact aatgtgtctg tacggaagaa gaattagaaa caaaaaatac 3481 tgcaaaaacc aaaagaacgg ggggataatt ccccccgttc tcgatgaaag agcactacac 3541 atagaaatag cgtgtggaaa ctgcatagaa tgtagacaaa aaagagcaag agagtggcaa 3601 gtaagactac aagaagatat aaaaaccaac aaaaacggga tattcataac actaacatta 3661 agcaacgaaa gttacacaga actaagcgga caagtaagta acaagataat aagagaacta 3721 aatacactat acaacatagg gtatgataac caacaaaaaa acaggaaaat aaaaaagcta 3781 gaagaaaagt taaaggggta cgcaaaagac aatgaaatag caaaaatggc aataagaaga 3841 ttcgtagaac gatggagaaa aaaattcaaa aaaagcgtaa gacactggct agtaacagaa 3901 ctaggacaca atggtacaga aaatatacac ctgcacggaa taatatggac aaacgaaagc 3961 agtgagacaa taaaagaaat atggaactac gggttcatat ggacgggata cgacaggtgg 4021 aaagggaaag aaaactatgt aaacgatgaa acaataaact acacagttaa atacatacac 4081 aaagtagact tcaaacacaa actatataaa agcatagtac taacaagcgc aggaatggga 4141 gctaactacg ttaactcaat aaatgcaaga aacaacaaat tcgcagataa agaaacagta 4201 gacaattaca gaacaaggaa cggacacaaa ctaccattaa caacatactg gaaaaacaaa 4261 ctatacacag aagcagaaaa agaaaagcta tggatgaaca aactagacga acaaaaagca 4321 tacgtatgcg gggaggaaat aaacacaaaa aacggatggc aagaatactg gaaagcaata 4381 aaacaccata ggctaagaaa caatagactc ggatacggaa gcgataaaaa aaactggcaa 4441 agagcatggt acgaagaaga aagaagaaag aaattgcaag aagccagaat gatataaaaa 4501 atacactaaa taccagaaag acttaaaaaa ccgtaccgac gtccagctag ggaatccggg 4561 ggctcacccc cgagccccga cccggtacga ccgggagcgg atttttttag gatatggatg 4621 gccggcgaaa accgccggaa gagcatacgc ccagcaacgg ctctcgccag aaaaaaaaaa 4681 ttggaaataa aaaaagaaac cgtaaattag cggattataa accgccgaaa ggcacaaaac 4741 taaaacaaat gaaaaaagca aacgcagaag taaacgttac aatcgtaaca caatcaggta 4801 atgacattct aaaaacaaaa gaaaaacaaa tgtactacct gaaaatagaa acagaagcag 4861 gagaagccct gctaaacgtt ggtgaaaaaa cctacaataa agtaaaattc attacagatg 4921 aaaacgaaat acaaaaaagt aaaaaactca acaccagctc taaacaacag tagagaaaaa 4981 ataacatgga aaagctccag tagatatgta gacatagaca cgggagaaga aataaaaaaa 5041 gaacaagtaa aaaacaagga atatataata ataacaaaaa caaaaactca taaaatatat 5101 ggaaagcaca gaacaaaagg acatattgaa tatgtctcag aatgtagacg aaaccctcaa 5161 agtaaaatct ggtgaactat tcacaagaca cgatatacag ggaacaccat tctacgcaat 5221 agagataaaa gacaaatggt ttctagcact aggaagatac aaaataagcg aagacttctc 5281 atgcatggaa gacattgaaa catacttaaa aaccaatatg tacgaagtgg tattagacat 5341 gatcacaaga gtatgggata tgtcactaga aatgaaaatg atggaaaaac ccatcaaaaa 5401 accaaaaaaa gttacaaaca agagttaaca aaaaaaagtc cccaaacggg gactttttta 5461 ttacttagct gttcatcaaa aaaacataaa atgaaaatct tagaaggaat caaaaaagta 5521 ttaaaaggag ttcaaaaatt cctaaactgg ttattcagca acacaagcaa accagcaaaa 5581 ccagagtaaa caaaagtaat taaccaaaaa aaaagaaac //