LOCUS MH617432 4364 bp DNA circular ENV 29-MAR-2019 DEFINITION Microviridae sp. isolate ctfa877, complete genome. ACCESSION MH617432 VERSION MH617432.2 DBLINK BioProject: PRJNA393166 BioSample: SAMN09074552 Sequence Read Archive: SRR7122375 KEYWORDS ENV. SOURCE Microviridae sp. (animal metagenome) ORGANISM Microviridae sp. Viruses; Microviridae; unclassified Microviridae. REFERENCE 1 (bases 1 to 4364) AUTHORS Tisza,M., Buck,C., Pastrana,D., Welch,N. and Peretti,A. TITLE Uncovering a Universe of Circular DNA Viruses in Animal Metagenomes JOURNAL Unpublished REFERENCE 2 (bases 1 to 4364) AUTHORS Tisza,M., Buck,C., Pastrana,D., Welch,N. and Peretti,A. TITLE Direct Submission JOURNAL Submitted (12-JUL-2018) Lab of Cellular Oncology, National Cancer Institute, 37 Convent Dr., Room 4118, Bethesda, MD 20814, USA COMMENT On Mar 29, 2019 this sequence version replaced MH617432.1. ##Genome-Assembly-Data-START## Assembly Method :: SPAdes v. 3.11.0 Genome Coverage :: 37.300871x Sequencing Technology :: Illumina ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..4364 /organism="Microviridae sp." /mol_type="genomic DNA" /isolate="ctfa877" /isolation_source="minnow tissue" /db_xref="taxon:2202644" /environmental_sample /country="USA" /collection_date="01-Jun-2017" /metagenome_source="animal metagenome" /note="genome binned from sequencing reads available in SRX4043871; metagenomic" CDS 1..1569 /inference="protein motif:CDD:222784" /codon_start=1 /transl_table=11 /product="major capsid protein" /protein_id="AXH76658.1" /translation="MKSVMTHQFSHVPKADIPRSTFNRSHGHKTTFDAGLLIPILVDE ALPGDTFNVNLAAFARLATPIFPLMDNMHMDTHFFSVPMRLVWENWQRFNGEQTTPGA STDYLVPQQIAPAGGYLEGSIHDYMGLPTKVAGYSHSALPLRAYNLIFNEWFRDQNLV VPGAQLIDDGPDPQNAYALRSRGKRHDYFTSALPWPQKGPGVTIPLGTSAPIFGAFKV KDSAATATGKLTAAPTSGNISWTATAPGTSQDIYANNADPNGLRADLSAASAATINSL RQAFQIQKIYERDARGGSRYVEILKAHFGVTSPDARLQRPEYLGGGSTPVNITPVTQN SPTGTYANTPQGNLAAFGTAGMSQHGFTASFTEHCIIMGFVSVRADLTYQQGLNRMWS RRTRFDFYWPALSHIGEQAVLQKEIFTDGIEANDDKVFGYQERYAEYRYFPSIITGLF RSNAAASLDSWHLSQEFATAPVLDNTFIRESPPIDRVIAVPTEPHFLFDSYFDMKCTR PMPVYGVPGLIDHF" CDS 1515..2498 /inference="protein motif:CDD:164789" /codon_start=1 /transl_table=11 /product="DNA pilot protein" /protein_id="AXH76659.1" /translation="MYPANARLRRARSHRPFLMGFFSKIKKGFKKVLPYAATAAGAAF GGPIGGAIGGSLFGKTGEKYGSMLGGALGNMAGDGFSSAQQLAATQYGYDQSLKGIRE QNWSARQIASDANKLSQQQANQQMLFQAGMSNTAHQREIKDLRRAGLNPILSGGGMGA STPQGAAGDVSVAPVRSEGDAISTAMDVFRTVAQAMKTNADREFVQSVATPKAQAETA KTKSETMPRIENIAKLKTQINAIATENYKNQMDADLKEQLRIESRKVVENLEKTGKNL DVTQRQMKAELQRMLADEEIYNGEAGYWIRLIEKLAPAAGKIPNVVLKKGR" CDS 2501..2950 /inference="protein motif:CDD:164781" /codon_start=1 /transl_table=11 /product="internal scaffolding protein" /protein_id="AXH76655.1" /translation="MSMNTGKTKLHKPEPFYSAYSTKLKMPITFQENSPWTDQSQKAD ADINTIMARYQSTGELPVINAAEANFLDVSEMDFRTHMDAVREANAMFAQLPSKIRDR FGNDPGAFLGFVEDEDNLPEIAKLGLLTQEATQKILYPNQPLTDEDA" CDS 3022..3378 /note="No conserved domains" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AXH76654.1" /translation="MSQRGILMASTLIILIFILEYFHHETTQNEQPQKQKAIHSDCLQ DSQIEFPGYPDEGWYTLIKCPATIREKLGDHQKPMLPGNGHWYLTIPRDSQPIPLSSP AGGVSDADSKGPEPGP" CDS 3210..4100 /inference="protein motif:CDD:222782" /codon_start=1 /transl_table=11 /product="replication initiator protein" /protein_id="AXH76656.1" /translation="MPCYHPREAWRSSETNASGKRPLVFDHTKGLSTHPVIIPCGGCI GCRLERARAWSLRLLHEKQDHELSIFVTLTYSDQYLPINRGLNPLHTQRFLKRLRKKH DAGVGNKLRFFLCGEYGEQTLRPHYHAILFGIDFPDKRKHSTNEQGDTLWTSETLEKT WGMGHCLTGNVTKQSCEYVARYILKKVNGELAEQHYQGREPEFIRMSRRPGIGKNYYD QYQKSLYTRDFVTSNGKQAPVPKYYDRLLEKDNPQLLEQKKIQRTKSALLRKADNTPE RLTVRKEVRQARIKQLTRKL" CDS 4102..4362 /inference="protein motif:CDD:222780" /codon_start=1 /transl_table=11 /product="nonstructural protein" /protein_id="AXH76657.1" /translation="MQKLLLAIFDIKSGVYSNPYTALNNDLALRDFTYACTQESDSEL YRYTKDFDLVKIGTYDDTTLEIIPDMQILLYGADIQNLPKGQ" ORIGIN 1 atgaaatcag taatgacaca ccaattctct catgtaccta aagcggatat tccgcgctca 61 acattcaacc gttcacacgg tcataaaaca acgttcgacg caggactctt aataccaatc 121 ctcgtcgacg aagcactacc cggcgatacg ttcaacgtaa atctcgccgc gttcgcccgt 181 ctcgctacac ccatatttcc tttgatggat aatatgcaca tggatacgca ctttttcagc 241 gtaccaatgc gcctcgtatg ggaaaactgg caacgcttca atggcgaaca aaccacaccc 301 ggcgccagca ccgattacct cgtgccgcaa caaattgctc cggccggtgg gtacctggaa 361 ggctccattc acgattacat gggccttcca acaaaagtag cgggctactc acattccgca 421 ctcccactgc gggcctataa cctaattttc aatgaatggt ttagggacca aaatctcgta 481 gtaccgggcg cacaactaat cgatgacgga ccggacccgc agaatgcgta tgccttacgc 541 tcaagaggca agcgacacga ctatttcacg tcggcgttac catggcccca aaaaggacca 601 ggcgtaacaa tcccattggg aacatcagca ccaatattcg gcgcattcaa agtaaaggac 661 tctgcagcga cagcaacagg gaaactcaca gcggccccca catcaggaaa tatatcttgg 721 accgcaacgg ctcccggaac atcgcaggac atctacgcaa acaatgcaga ccctaacgga 781 ctacgcgcgg atttaagcgc agcatcagca gcaacgataa actcactgcg gcaagcattc 841 cagatccaaa aaatctacga gcgagatgca aggggggggt cacgctacgt ggaaattctc 901 aaggctcact tcggagtaac gtcccccgac gcccgtttgc aacggcctga atatctaggg 961 gggggtagta ccccagtaaa cataacccca gtcacacaaa atagtccgac tggcacttac 1021 gccaatacgc cccaaggcaa tctcgcggca ttcggaaccg cgggtatgtc tcaacacggc 1081 ttcacagcat catttaccga acactgcatc ataatgggct tcgtatccgt ccgggccgac 1141 ctcacctatc agcagggcct aaaccgcatg tggtcacgtc gaacacgttt cgacttttac 1201 tggccagccc tgtcacatat cggggaacag gctgtactgc agaaagaaat cttcacagac 1261 gggatcgagg ctaacgacga taaagtcttc ggataccagg aacgctatgc ggaataccgc 1321 tacttcccgt caataataac cggactgttc cggtcaaacg ccgcagcctc gctcgattct 1381 tggcaccttt cgcaggaatt cgcaacggca ccggtcctgg ataacacctt catcagggaa 1441 tcaccgccca tcgatcgagt aatagcggta cccactgaac cccatttcct attcgattcg 1501 tacttcgaca tgaaatgtac ccggccaatg cccgtctacg gcgtgcccgg tctcatagac 1561 catttctaat gggattcttc agcaaaatca aaaagggctt taaaaaggtc cttccctatg 1621 ccgcaaccgc tgccggggcc gcctttggcg gtcctatcgg cggggcaatc ggcggctcgc 1681 tattcggcaa aaccggtgaa aaatacggtt caatgctcgg aggcgctctc ggaaatatgg 1741 ccggagacgg ctttagctct gctcaacaac tggcggcaac gcaatacggt tacgaccagt 1801 cactaaaagg cataagggaa cagaactgga gcgcgaggca aattgcctca gatgccaaca 1861 aactatccca acaacaagcc aaccagcaaa tgctcttcca agcgggcatg agcaacaccg 1921 ctcaccaacg tgagatcaaa gacctacgcc gtgcgggcct caatccaatc ctgagcggtg 1981 gagggatggg cgctagtacc ccacagggtg ccgcaggcga cgtctccgtg gctcctgtgc 2041 ggtctgaggg ggatgcaata tccaccgcta tggacgtctt caggactgtt gcacaggcaa 2101 tgaagaccaa cgcggaccgg gaattcgtac aatccgtagc aacacccaaa gcgcaagcgg 2161 aaacagcgaa aaccaaatcc gagaccatgc cacgcataga aaacatcgca aaactcaaga 2221 cacagatcaa tgcgatagca acagaaaact ataaaaatca aatggatgcg gacttaaaag 2281 agcaattaag aatcgaatcc cgcaaagtcg tagagaacct cgaaaagacc ggcaaaaatc 2341 tggatgtaac ccaacgccag atgaaagccg aactccaaag gatgcttgca gacgaagaaa 2401 tctacaacgg cgaagccggt tactggattc gcctcataga aaaactagcc ccggcagccg 2461 ggaaaatacc caacgtagta cttaaaaaag gacgataatc atgagcatga atacaggcaa 2521 aaccaagcta cacaagccag aacccttcta cagcgcatac agcaccaagc tgaaaatgcc 2581 tataaccttc caagagaact caccttggac agaccaaagc caaaaagcgg acgcggacat 2641 aaacaccatc atggctcgct atcagtcgac cggggaacta ccggtcatca acgcagctga 2701 agcaaacttc ctggatgtct cagaaatgga cttcagaacc cacatggacg ccgtacgtga 2761 ggccaacgca atgttcgccc aactgccgtc caaaatacgc gaccgcttcg gaaacgaccc 2821 aggcgcgttc ttaggcttcg tagaagacga agacaacctg cccgaaattg caaaattagg 2881 gctactaacc caagaggcaa cccaaaaaat cctctatcca aatcagcctc ttaccgacga 2941 agatgcttga catcgatcgt caaaatggag tattgatatc ttgataccaa tactcccact 3001 gacaccaaag tcagaaaaac tatgtcacaa cgtggaatct taatggcatc aaccttaatt 3061 attctcattt ttatactgga gtactttcac catgaaacga cacaaaatga acaaccacaa 3121 aagcaaaagg ctattcacag cgactgcctc caggactcac aaattgaatt tccggggtac 3181 cccgatgagg ggtggtatac gcttataaaa tgccctgcta ccatccgaga gaagcttgga 3241 gatcatcaga aaccaatgct tccgggaaac ggccactggt atttgaccat accaagggac 3301 tctcaaccca tcccgttatc atcccctgcg gggggtgtat cggatgccga ctcgaaaggg 3361 ccagagcctg gtccttaaga ctacttcacg aaaaacagga ccacgagctg tcaatcttcg 3421 tgaccctgac atactccgat caataccttc ctatcaacag gggtttaaat cccctacaca 3481 ctcaacgctt tctcaagcgt ttaaggaaaa agcacgatgc aggagtcgga aacaaactca 3541 gattcttcct ctgtggagaa tacggagaac agacgcttcg tcctcattac cacgcaatcc 3601 ttttcgggat tgacttcccg gacaagcgca aacattcaac caatgaacaa ggagatactc 3661 tatggactag tgaaacactc gaaaaaacat ggggaatggg ccattgcctt acgggtaatg 3721 ttactaaaca atcctgcgaa tacgtagccc gttacatcct caaaaaagtg aacggcgaac 3781 tcgcagaaca acactaccaa gggcgcgaac ccgaattcat tcggatgtct cgtcgacccg 3841 gaataggaaa aaattattat gaccaatatc aaaaaagcct ctacacacgg gacttcgtca 3901 cgtccaatgg caaacaagct ccggtcccca aatactatga ccggctactt gaaaaagata 3961 atcctcaact acttgaacaa aagaaaatac aaagaactaa atcggccctt ctaagaaaag 4021 cagataacac acccgaacgg ctcaccgttc gaaaagaagt cagacaggct cgaatcaaac 4081 aactaacaag gaaactctaa aatgcaaaaa ctactactag caatattcga tatcaaatca 4141 ggcgtctatt ctaatcctta cacagcacta aacaatgacc tcgcactgcg agactttacc 4201 tacgcctgca cacaagaatc agattcagag ctgtaccgtt atactaaaga cttcgatctc 4261 gtcaaaatag gaacctatga cgacaccaca ctggaaatca ttccagacat gcaaatcctg 4321 ctctatggag cagatattca aaacctacct aaaggacaat aaac //