LOCUS MH617316 4686 bp DNA circular ENV 29-MAR-2019 DEFINITION Microviridae sp. isolate ctfh688, complete genome. ACCESSION MH617316 VERSION MH617316.2 DBLINK BioProject: PRJNA393166 BioSample: SAMN09074552 Sequence Read Archive: SRR7122375 KEYWORDS ENV. SOURCE Microviridae sp. (animal metagenome) ORGANISM Microviridae sp. Viruses; Microviridae; unclassified Microviridae. REFERENCE 1 (bases 1 to 4686) AUTHORS Tisza,M., Buck,C., Pastrana,D., Welch,N. and Peretti,A. TITLE Uncovering a Universe of Circular DNA Viruses in Animal Metagenomes JOURNAL Unpublished REFERENCE 2 (bases 1 to 4686) AUTHORS Tisza,M., Buck,C., Pastrana,D., Welch,N. and Peretti,A. TITLE Direct Submission JOURNAL Submitted (12-JUL-2018) Lab of Cellular Oncology, National Cancer Institute, 37 Convent Dr., Room 4118, Bethesda, MD 20814, USA COMMENT On Mar 29, 2019 this sequence version replaced MH617316.1. ##Genome-Assembly-Data-START## Assembly Method :: SPAdes v. 3.11.0 Genome Coverage :: 92.568502x Sequencing Technology :: Illumina ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..4686 /organism="Microviridae sp." /mol_type="genomic DNA" /isolate="ctfh688" /isolation_source="minnow tissue" /db_xref="taxon:2202644" /environmental_sample /country="USA" /collection_date="01-Jun-2017" /metagenome_source="animal metagenome" /note="genome binned from sequencing reads available in SRX4043871; metagenomic" CDS 1..1638 /inference="protein motif:CDD:222784" /codon_start=1 /transl_table=11 /product="major capsid protein" /protein_id="AXH76128.1" /translation="MFRNKSVDVHQFAMVPKSDVPRSVFNRQSTHKTTFDSAYLIPVF CDEVLPGDSFNLKMTAFARLSTPIFPIMDNLYLDSFFFFVPCRLVWNNWKKFMGEQST PGDSISYVIPQLVSPPGGFVVGSIFDYFGLPTVGQVDAGNTVSVNALPLRCYNIIYNE WFRDENLQNPNFLQLNDGPDVLTSYSLTRRGKRHDYFTSALPWPQKGTAVSLPLGTSA PIKTSSAVQQTGAQSAMGFNLASSGASAGTFQLGTVAGAASVNATAFAGSTGGVYPNN LYADLSTATAATINQLRQSFQIQKLLERDARGGTRYTELLRAHFGVMSPDARLQRPEY LGGGTTAVNISPIAQTSGSPIPSGYTTTPMGTLAAMGTALASGHGFTQSFTEHGYIIG LVSIRADLTYQQGLRKMWSRSTRYDFYWPEFAMLGEQTISNREIYCKGDQNANDALVF GYQERWAEYRYLPSMITGLFRSTSAGTIDGWHLAQNFTTLPTLNGTFISENPPLSRVL AVGAAAAGQQIIFDSFFQIKTVRPMPLYSVPGLIDHF" CDS 1648..2739 /inference="protein motif:CDD:164789" /codon_start=1 /transl_table=11 /product="DNA pilot protein" /protein_id="AXH76129.1" /translation="MGLFSSIGKALKGVAKVALPAAAAYGTAELFGAPSLFGSSMLGS TNIFGGSLYSDSLSGGIFSGIGNLLSNSTAQNALGAYGAYAGTQATNAQQLASTREQM DFQAAEAQKARDYNTTMSNTSWQRGVSDMRSAGLNPMLAYSQGGASAPTSPMGQGASP PSLRSPSLEAINTGMALKSQAANIENVSSQTDFNKANTAKSVVEANLVNAQIDKVRSE TQGQLNVNSRFDFEKAYRWFYEPNNWMSEAHVKDLDSKHAEVLLKAIDEMNEWASEQT GGRMNAAAYAESIGIRNDRADARIRELDIERAKREAQMYKSPEGKYIPYIHSGSQAAS GVGALGIGAGLFRGSRSGFEFKPSFNISK" CDS 2489..2752 /note="No conserved domains" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AXH76124.1" /translation="MLLLMLSLLVFVMIVPMRVFASWTLSVLSVRLRCISLLRVSISL IFILVLRLLLVLVLLVLVPVCFVVLVLVLNLSLLLIFLSDWSL" CDS 2752..3216 /inference="protein motif:CDD:164781" /codon_start=1 /transl_table=11 /product="internal scaffolding protein" /protein_id="AXH76130.1" /translation="MDKPTVKPVFDVVVPFIRTGFNYDMNAVSVDTGLACTDLSLAVQ SEADECDINTIVRRFGLTGQLPVGVRMPTYGDFTGVFDFASAANAIALANESFDEMPA EVRARFENDPAKFVDFCSDDGNRAEAERLGLVPVATSVSASVVSKVDTSEIS" CDS 2972..3277 /note="No conserved domains" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AXH76125.1" /translation="MVILLVYLILLLLLMLLLWLMSLLTKCLLRFVLVSRTILLSLLI FVPTTGTGLRRNASVLSPLRQALARQLFRKLTLRKFHDIRPACYLIMLVWVDVVSTF" CDS 3556..4422 /inference="protein motif:CDD:222782" /codon_start=1 /transl_table=11 /product="replication initiator protein" /protein_id="AXH76131.1" /translation="MACFHPLQAWKHFNGEVSFRKEAHALSHEISLPCGNCVGCRLER SRQWSVRIMHEASLHDKNCFITLTYDEAHVPFDSGLHYEDFQCFMRKLRRTDKVRFFM CGEYGSLRLRPHFHACLFGIDFDDGKPVAAAASGGFIYSSDKLSVLWPQGYCSVGELT HASAAYVARYTLKKSSNPRVYDFLDVSTGEVISRVPEFVRMSLKPGIGSDWYDKFKSD VYPHDRVIVNGRAVKPPKYYDRKDADFSNSITSLKRWEFAQAHLDDQTPERLSVREAV VKSRLSHLKRSL" CDS 4424..4684 /inference="protein motif:CDD:222780" /codon_start=1 /transl_table=11 /product="nonstructural protein" /protein_id="AXH76127.1" /translation="MRYQMFSVFDIALGTFGRPMFLQSTGQAIRSFSDEVNRSADDNQ MNKHPVDFSLFHLGEFDDDAAEFSIFPKPERLALAADLLIKE" ORIGIN 1 atgtttcgta ataagtctgt ggatgtgcac cagtttgcta tggtgcctaa gtccgatgtt 61 ccaaggtctg tttttaatcg tcaatctact cataagacga cttttgattc tgcgtatttg 121 atacctgttt tttgtgatga agttttgccc ggtgattctt ttaatttgaa gatgactgct 181 tttgctcggt tgtctacgcc tatttttccg atcatggata atctttattt ggattctttt 241 ttcttttttg ttccttgccg gcttgtttgg aataattgga agaagtttat gggcgaacag 301 tctactcccg gtgattctat ttcttatgtg attcctcagc ttgtttcacc tcctggtggt 361 tttgttgttg gttctatttt tgattatttt ggtttgccta ctgtaggtca agttgatgct 421 ggtaatactg tttcggttaa tgctttgcca ttacgttgtt ataacattat ttacaatgag 481 tggtttcgtg atgaaaatct tcagaatcct aactttcttc aacttaatga tgggccggat 541 gttttaacga gttattcttt gactcgtcgt ggtaaacgcc atgattattt tacatctgct 601 ttgccttggc ctcagaaagg tactgctgtc tctcttcctt taggtacttc tgctcctatt 661 aagacatctt ctgctgttca acagactggt gctcagtctg ctatgggttt taatttagct 721 tctagtggtg cttctgctgg tacttttcaa ttaggtactg ttgcaggtgc tgctagtgtt 781 aatgcgacgg cttttgctgg ttctactggt ggtgtttatc ctaataatct ttatgctgat 841 ctgagtactg cgactgctgc gacgattaat caattgcgtc aatcttttca gattcagaag 901 ttgcttgagc gtgatgcgcg tggtggtact cgctatacgg agttattgcg tgcgcatttc 961 ggtgtgatgt ctcccgatgc taggctccag cgtccagagt acttgggagg tggtactacc 1021 gctgtgaata tttctcctat tgctcaaact tctggttctc ctatcccatc tggttatacg 1081 actactccta tgggtacttt ggctgctatg ggtactgctt tggcttctgg tcatggtttt 1141 actcagtctt ttactgagca tggttatatt attggattgg tcagtattcg tgctgatctt 1201 acttatcagc agggtttgcg taagatgtgg tcgcgttcta ctcgttatga tttttattgg 1261 cctgagtttg caatgcttgg agagcaaact atttctaatc gtgagattta ttgtaagggt 1321 gatcagaatg ctaatgatgc gcttgtgttt ggttatcagg agcgctgggc tgaatatcgt 1381 tatttaccgt ctatgataac tggtcttttt cgatctactt ctgccggcac tattgacggt 1441 tggcatttgg ctcagaattt tacgactttg cctactttga atggtacttt tatttctgag 1501 aatccgcctt tgtctcgtgt tttggctgtt ggtgctgctg ctgctggtca gcagattatt 1561 tttgattcgt tttttcagat taagacagtt cgtcctatgc ctttgtattc tgttcctggt 1621 ttgattgacc atttttagga gtttattatg ggtctttttt cctctattgg taaggctctt 1681 aagggtgtcg ctaaggttgc tttgccggct gctgctgcct atggtactgc tgagttattt 1741 ggtgctcctt ctttgtttgg ttcttcgatg ttaggtagta ctaatatatt tggaggttct 1801 ttatattctg attctttatc tggtggtatt ttttctggta ttggtaattt gttgtctaat 1861 tctactgctc aaaatgcttt aggtgcttat ggtgcttatg ctggtactca ggctactaat 1921 gctcaacagt tggcttcaac tcgtgagcag atggattttc aagctgctga ggctcagaaa 1981 gcgcgtgatt ataatacaac tatgtctaat acttcttggc agcgtggtgt gtctgatatg 2041 cgttctgctg gtttgaatcc tatgttggct tattctcagg gcggtgcttc tgcgcccact 2101 tctcctatgg gtcaaggcgc gtcccccccc tccttgcggt ctccctcgct ggaagctatt 2161 aatactggta tggctttgaa gtctcaggcc gctaatattg agaatgtttc ttctcagact 2221 gattttaata aggctaatac tgctaagtct gttgttgaag ctaatttggt taatgctcag 2281 attgataagg ttagatcaga aacccagggt cagttgaatg ttaattctcg ttttgatttt 2341 gagaaagcgt atcgttggtt ttatgagcct aataattgga tgtctgaggc gcatgttaag 2401 gatttggatt ctaaacatgc cgaggttttg cttaaggcga ttgatgaaat gaatgaatgg 2461 gcttctgaac agactggtgg tcgtatgaat gctgctgctt atgctgagtc tattggtatt 2521 cgtaatgatc gtgccgatgc gcgtattcgc gagttggaca ttgagcgtgc taagcgtgag 2581 gctcagatgt ataagtctcc tgagggtaag tatatccctt atattcattc tggttctcag 2641 gctgcttctg gtgttggtgc tcttggtatt ggtgccggtt tgtttcgtgg ttctcgttct 2701 ggttttgaat ttaagccttc ttttaatatt tctaagtgat tggagtttgt aatggataag 2761 cctactgtta aacctgtttt tgatgttgtt gttcctttta ttcgtactgg ttttaattat 2821 gatatgaatg ctgtttctgt tgatacaggt cttgcctgta ctgatttgtc tttggctgtc 2881 cagtctgaag ccgatgaatg tgatattaat actatagttc ggcgttttgg tttgactggt 2941 cagttacctg ttggtgtgcg tatgcccacc tatggtgatt ttactggtgt atttgatttt 3001 gcttctgctg ctaatgctat tgctttggct aatgagtctt ttgacgaaat gcctgctgag 3061 gttcgtgctc gtttcgagaa cgatcctgct aagtttgttg atttttgttc cgacgacggg 3121 aacagggctg aggcggaacg cctcggtctt gtccccgttg cgacaagcgt tagcgcgtca 3181 gttgtttcga aagttgacac ttcggaaatt tcatgatata agaccagcgt gttacttgat 3241 tatgctggtc tgggtggacg tagtttctac gttctaaccc tgtttttagg ttaacccttt 3301 cttttaaaag gagtttttat gtgggattgt cctaatcgtg agcgttctga gatttggttt 3361 aagcgaatgt ctgagagttg tttgatcggt gattgggatt ctgtccgttt ttggttgtct 3421 aatcttgaga ggagtatttt gtatgcatcg ccatcgtgtt aataagcaca agagtgctcg 3481 taagtttcgt catcaggtag gccatactaa ggctgctaat atgcgtggtc ctatgcgtgg 3541 tggtattagg ttgtaatggc ttgttttcat cctttgcagg cgtggaagca ttttaatgga 3601 gaggtatctt ttcgtaagga ggcgcatgcg ctttcccatg agatttctct tccttgtggt 3661 aattgtgtcg gttgtcgttt ggagcgttct cgtcagtggt cggttcggat aatgcatgag 3721 gcttctcttc atgataagaa ttgttttatt acattgactt atgatgaggc tcatgtccct 3781 tttgattctg gtcttcatta tgaagatttt cagtgtttta tgcgtaagct taggcgtacc 3841 gataaggtgc gcttttttat gtgtggggag tatggctcgt taaggcttcg ccctcatttt 3901 catgcttgtc tttttggaat tgattttgat gatggtaagc ctgttgctgc tgctgcttct 3961 ggtggattta tttactcttc cgataagctc tcggttttat ggcctcaagg ttattgttct 4021 gttggtgaac tcactcatgc ttctgctgct tatgtcgctc gttacacttt gaagaagtct 4081 tctaaccctc gtgtttatga ttttttggat gtatctactg gtgaggttat ttctcgtgtt 4141 cctgagtttg ttcgtatgtc tttgaagcct ggtattggtt ctgattggta tgataagttt 4201 aagtcggatg tttatccgca tgatcgtgtg attgtgaatg gccgtgctgt taagccccct 4261 aagtattatg atcgtaagga tgctgatttt tctaattcaa tcacctcgct taaaaggtgg 4321 gagtttgctc aggcgcattt ggatgatcag acgcctgagc gtttgtctgt tcgtgaagct 4381 gttgttaaat ctcgtttatc tcatttaaag aggagtttat gatatgcgtt atcaaatgtt 4441 ttctgtcttt gacattgctt taggtacctt tggtcgtcct atgttcttgc aatctactgg 4501 tcaggctatt cgttcttttt ctgacgaggt gaatcgttct gcagatgata atcagatgaa 4561 taaacaccct gttgattttt ctttgtttca tttgggtgaa tttgatgatg atgctgcaga 4621 gttttctatt tttcctaagc ctgagcgtct ggctcttgcg gctgatttgt taattaagga 4681 gtgatc //