LOCUS MH622930 4408 bp DNA circular ENV 29-MAR-2019 DEFINITION Microviridae sp. isolate ctfj846, complete genome. ACCESSION MH622930 VERSION MH622930.2 DBLINK BioProject: PRJNA393166 BioSample: SAMN09074552 Sequence Read Archive: SRR7122375 KEYWORDS ENV. SOURCE Microviridae sp. (animal metagenome) ORGANISM Microviridae sp. Viruses; Microviridae; unclassified Microviridae. REFERENCE 1 (bases 1 to 4408) AUTHORS Tisza,M., Buck,C., Pastrana,D., Welch,N. and Peretti,A. TITLE Uncovering a Universe of Circular DNA Viruses in Animal Metagenomes JOURNAL Unpublished REFERENCE 2 (bases 1 to 4408) AUTHORS Tisza,M., Buck,C., Pastrana,D., Welch,N. and Peretti,A. TITLE Direct Submission JOURNAL Submitted (12-JUL-2018) Lab of Cellular Oncology, National Cancer Institute, 37 Convent Dr., Room 4118, Bethesda, MD 20814, USA COMMENT On Mar 29, 2019 this sequence version replaced MH622930.1. ##Genome-Assembly-Data-START## Assembly Method :: SPAdes v. 3.11.0 Genome Coverage :: 18.523367x Sequencing Technology :: Illumina ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..4408 /organism="Microviridae sp." /mol_type="genomic DNA" /isolate="ctfj846" /isolation_source="minnow tissue" /db_xref="taxon:2202644" /environmental_sample /country="USA" /collection_date="01-Jun-2017" /metagenome_source="animal metagenome" /note="genome binned from sequencing reads available in SRX4043871; metagenomic" CDS 1..1578 /inference="protein motif:CDD:222784" /codon_start=1 /transl_table=11 /product="major capsid protein" /protein_id="AXH72191.1" /translation="MFGSNHRNPSVMAHTFSQVPKADIPRSSFDRSHGYKTTFDAGYL IPIFVDEALPGDTFNLRMTGFSRLATPIYPLMDNMYMDTQFFSVPVRLVWDNWQRFNG EQPNPGDSTDFTIPQMVAPAGGYAENSIHDYMGLPTGVAGISHSALWHRAYALIWNEW YRDQNMQDAIAVPKGDGPDLHTFYPLMKRGKRHDYFTSSLPWPQKGEAVQIPLGASAP INASGNFQMHSSGTPSAHGTLATNGTGQLVATGTSNNDYLVYDSGLIADLSSATAATI NSLRQAFQIQKIFERDARGGTRYTELIRSHFGVTSPDARLQRPEYLGGGSTPVNISPI PQTSPTGTYANTPQGNLAAIGTALVSGHGFTTSFTEHCLIIGIVSVRADLTYQQGLNR MWSRKTRFDFYWPALSHIGEQAVLQKEIFADGIEANDDKVFGYQERYAEYRYKPSQIT GLFRSQAATSLDAWHLSQEFATAPVLDDDFITENPPIDRVIAVPSEPHFLMDAYFKLR CTRPMPVFGVPGLIDHF" CDS 1581..2402 /inference="protein motif:CDD:164789" /codon_start=1 /transl_table=11 /product="DNA pilot protein" /protein_id="AXH72192.1" /translation="MADDSPLVIDIPGAGGTAAAAVESAFPWGPLIAGGASILGGLIS NDSSAKSAARQMQFQREANQKSMDFSASQSQIQRDYQERLSNTAHQREVSDLRAAGLN PILSVNRSGAGTPSGAAGTGVTSAGSSYKAENIASGAASTAVSAMRTMAELDQIQAIT ERTREEAALTRQNTSNASVDYNTKLEQAALTKAQTDVALKSLGYTDAQIKAIETGIPL TQAQTALTSSTAKSAAIEANLDASMRAPERGIAMAQGGTSAVRNLINPFPSFGKK" CDS 2427..3005 /inference="protein motif:CDD:164781" /codon_start=1 /transl_table=11 /product="internal scaffolding protein" /protein_id="AXH72193.1" /translation="MLNPGTGLGIQQAKRASLKSSEGSQSMSLTQRKLKNHTQQSPNL QLPYSPSTLNHSITFKPDSPFTKQSFKDECDINTIMSRYQSTGVLPDLAQTAPQYLDA SGFDFQASMDYIANAHTLFHELPSSVRTKFGNDTASFMEFVSNPNNRSEMASMGLLRP DYAPPLGDDVSKPSEALKTPLSSNPASEPKGA" CDS 3243..4151 /inference="protein motif:CDD:222782" /codon_start=1 /transl_table=11 /product="replication initiator protein" /protein_id="AXH72189.1" /translation="MPCYHPIQGYRSQTLTKNGKRKLSFSPQTGYVDLPVTVPCGQCI GCRLERSRQWAIRCVHEAQLHELKCFITLTYDDASLPPGRSLVKQDFQKFMKRLRKET GLKIRYFHCGEYGETDQRPHYHAILYGIDFPDKKFYTRNKQKDTLYTSETLDRIWSKG KCWIGNVTFESAAYVARYVLKKVTGEAAIDHYKSVDPNTGEIFDRLPEYITMSLKPGI GGDWYDKYKGDVYPSDFHVLRGKTMRPAKFYDRQLEKTDPAALKKIKYKRIRSASKHK ADQTPERLAVRKEVKLSQIKSLSRNL" CDS 4151..4405 /inference="protein motif:CDD:222780" /codon_start=1 /transl_table=11 /product="nonstructural protein" /protein_id="AXH72190.1" /translation="MLKAFSIFDNQTNVFHQPFFVEHHQVALRYFVSLCRDEKSDICK FPAAFNLFELGSFDEQSGEFLALEQKVNHGFASAYINQEK" ORIGIN 1 atgttcggct cgaatcatcg caatccttct gtgatggcac acacattcag ccaagttcca 61 aaggctgata ttcctcgatc ctccttcgat cgttctcacg gctataaaac cacattcgac 121 gccggctacc tcattccgat cttcgtcgat gaggctcttc ctggcgatac cttcaacctt 181 cgaatgaccg gcttcagccg tctcgcaaca cccatctacc ctctgatgga caatatgtat 241 atggacacgc aattcttcag tgttcctgta cgtctcgtct gggataactg gcaacgcttc 301 aatggcgaac agcccaatcc cggcgattca accgatttca ctatcccgca aatggtcgct 361 ccagctggtg gctatgccga aaactctatc cacgactaca tgggtctgcc cactggtgtc 421 gccggcatct ctcactctgc cctctggcat cgcgcttatg ccctcatctg gaatgagtgg 481 taccgcgacc aaaacatgca ggatgcaatt gccgtcccga aaggtgacgg ccccgatttg 541 cataccttct atccccttat gaaacgtggc aagcgccacg actatttcac cagttcgctc 601 ccttggccac aaaagggcga agctgttcaa atccctcttg gtgcttctgc acccatcaac 661 gctagtggca actttcaaat gcacagctcg ggaaccccgt ctgcgcatgg cactcttgcc 721 accaatggca cgggtcagct ggttgctaca ggcacatcca acaacgacta tctagtctat 781 gactctggtc tcattgctga cctcagttca gcaacagctg caactatcaa cagccttcgt 841 caagctttcc aaatccagaa aatttttgaa agggacgctc gtggcggtac tcgatacact 901 gagctcattc gctctcactt tggcgttacg tctcctgacg cacgcctcca aaggcccgaa 961 tacctgggcg gaggctcgac gccagtcaac atatctccga tcccccaaac gtcgccgaca 1021 ggcacgtacg caaacactcc tcaaggcaac ctcgccgcaa ttggcactgc ccttgtctcc 1081 ggacatggct tcacaacctc cttcaccgaa cactgcctca tcatcggcat tgtctccgtc 1141 cgcgcagacc tgacctatca acaaggtctc aaccgcatgt ggtcgcgtaa aactcgcttc 1201 gatttctact ggcctgctct ctcgcacatt ggcgagcaag cggttcttca aaaagaaatc 1261 ttcgctgatg gcatcgaagc caacgacgac aaggttttcg gctaccaaga acgctatgcc 1321 gaataccgct acaaaccctc tcaaattacg ggactgttcc gcagtcaggc ggctacgtct 1381 ctggatgctt ggcatctctc gcaagaattt gcgactgccc ctgttctcga tgacgatttc 1441 atcaccgaga acccacctat agaccgcgtc atcgcggttc cttccgaacc tcacttcctc 1501 atggacgctt atttcaagct tcgctgtact cgtcctatgc ctgtgttcgg agtccctggt 1561 cttattgacc acttttaatc atggcggacg attcccctct cgttatcgac attcccggcg 1621 ctggtggtac cgctgccgcg gctgtcgaat ccgccttccc ttggggcccc ctcattgctg 1681 ggggggcctc tatcctgggg gggcttatct ctaatgactc ttcggctaaa tctgccgcac 1741 gtcaaatgca attccagcgg gaagccaatc aaaaatcaat ggacttctcc gcgtctcaat 1801 cgcaaattca acgcgattat caagagcggc tttcaaatac cgctcatcaa cgggaagtat 1861 cggatctgcg agctgcaggt cttaatccga tcctctccgt aaacagatct ggtgctggca 1921 ccccttctgg ggctgctggt accggcgtta catccgccgg cagctcctac aaggcagaaa 1981 acatcgcctc tggcgctgcc tcaaccgccg tatccgctat gcgtactatg gcggaactcg 2041 atcaaattca agctatcacc gagcgtactc gcgaggaagc agctcttact cgtcaaaaca 2101 ccagcaatgc ctcggtcgat tacaatacga agcttgaaca agctgcgctg acgaaggctc 2161 aaactgacgt cgcgcttaag agcctcggat ataccgatgc tcaaattaaa gctatcgaga 2221 ccggcattcc cttaactcag gctcaaacag ccttaacatc ttcaaccgca aaatctgcgg 2281 ccatcgaggc taacctcgat gcttcaatgc gtgcgcctga gcgaggaatc gccatggcgc 2341 aaggcggtac ctccgccgtc cgcaacctca tcaatccttt cccgtctttc gggaaaaaat 2401 aaatcaggct ttgcgccttt agcaaaatgc tcaacccggg taccgggttg ggcattcagc 2461 aagcgaagcg cgctagtctc aaatcatctg aaggatctca atcaatgtct ctcactcaac 2521 gcaaactcaa aaaccacact caacaatcac ccaatctcca actaccttac tcaccttcaa 2581 cactcaatca ctcaatcacc ttcaaacctg attcaccatt cacaaaacaa tccttcaaag 2641 atgaatgcga catcaacacc atcatgagtc gctatcaatc aaccggggtt ctccccgatc 2701 tggcgcaaac tgcgcctcaa tatctcgatg cctctggctt cgacttccaa gcgtcgatgg 2761 actacatcgc aaacgctcat acgctctttc acgagctacc ctcttccgta cgaacgaagt 2821 tcggcaacga cacggcctcc ttcatggagt tcgtgtctaa ccctaacaac cgttcagaaa 2881 tggcctctat gggcctcctg agacccgatt acgccccccc gctgggcgac gatgtctcta 2941 aaccctccga ggccctcaaa acgcctctat cctccaatcc tgcctcggaa ccaaaaggcg 3001 cttgacaagc gcccctggca cagttatttt ccttgttgta actgtgctag gtgacaccaa 3061 cccaatgctc acctctctcc aaacgcttat cttcctgttt ttcctcctga aagggtctcc 3121 aaatgaaacg ccacaaaatg tcgtccaaat cttcccgccg gcagttctct gcgaccgctt 3181 ctcgaaccca tgtcaagaat gttcggggcg gcccgatgag gggcgggatc cgcctgtgag 3241 cgatgccctg ctaccatccg atacagggct accgctctca aaccctgacg aaaaacggta 3301 agcgcaaact ctccttcagc ccccaaactg gctatgtcga tcttcccgtt accgtcccct 3361 gcggacaatg tattggatgc cgcctcgagc ggtctcgaca atgggccatt cgatgcgtcc 3421 acgaagcaca actgcacgag ctcaagtgct tcattaccct tacctacgat gacgcgtccc 3481 ttcctcctgg gcgctctctc gttaagcaag actttcagaa attcatgaaa cgtctacgca 3541 aagaaacggg gctgaaaatc cgttacttcc actgcgggga atacggggaa acggatcaac 3601 gtcctcacta tcatgcgatc ctctacggga tcgacttccc cgacaaaaag ttttacaccc 3661 gcaacaaaca aaaggacact ctctatacgt ccgaaaccct tgaccgcatc tggtcaaaag 3721 gtaaatgctg gatcggcaat gtcacattcg aatccgctgc ctacgtcgct cgctatgtcc 3781 tcaaaaaggt cactggcgaa gctgcaatcg accactacaa atcggtcgat cctaataccg 3841 gcgaaatttt cgaccggctt cccgaataca tcaccatgtc ccttaagccg ggaatcggcg 3901 gggactggta cgacaaatac aaaggagacg tctatccgtc tgacttccat gtcctgcgcg 3961 gtaaaactat gcggcctgct aagttctatg atcggcaact tgaaaaaacc gatcctgccg 4021 ctctcaaaaa aatcaagtac aaacgcatcc gctcagcttc aaaacataaa gctgatcaaa 4081 cacccgagag gcttgctgta agaaaagaag tcaagctctc gcaaatcaaa tctctctcaa 4141 ggaatctcta atgctcaaag ccttctcgat cttcgacaat caaaccaacg ttttccatca 4201 gccgttcttc gtcgaacacc atcaagtcgc tcttcgctac tttgtttctc tctgtcgtga 4261 tgaaaaatcc gacatctgca aattccctgc tgctttcaat ctgtttgaac tcggatcttt 4321 cgatgagcag tccggcgaat tcctcgctct cgaacaaaag gtaaatcacg gctttgcctc 4381 tgcctatatc aatcaggaga aataaacc //