LOCUS MH616697 5291 bp DNA circular ENV 05-AUG-2018 DEFINITION Microviridae sp. isolate ctha589, complete genome. ACCESSION MH616697 VERSION MH616697.1 DBLINK BioProject: PRJNA393166 BioSample: SAMN09074552 Sequence Read Archive: SRR7122375 KEYWORDS ENV. SOURCE Microviridae sp. (animal metagenome) ORGANISM Microviridae sp. Viruses; ssDNA viruses; Microviridae; unclassified Microviridae. REFERENCE 1 (bases 1 to 5291) AUTHORS Tisza,M., Buck,C., Pastrana,D., Welch,N. and Peretti,A. TITLE Uncovering a Universe of Circular DNA Viruses in Animal Metagenomes JOURNAL Unpublished REFERENCE 2 (bases 1 to 5291) AUTHORS Tisza,M., Buck,C., Pastrana,D., Welch,N. and Peretti,A. TITLE Direct Submission JOURNAL Submitted (12-JUL-2018) Lab of Cellular Oncology, National Cancer Institute, 37 Convent Dr., Room 4118, Bethesda, MD 20814, USA COMMENT ##Genome-Assembly-Data-START## Assembly Method :: SPAdes v. 3.11.0 Genome Coverage :: 79.539407x Sequencing Technology :: Illumina ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..5291 /organism="Microviridae sp." /mol_type="genomic DNA" /isolate="ctha589" /isolation_source="minnow tissue" /db_xref="taxon:2202644" /environmental_sample /country="USA" /collection_date="01-Jun-2017" /note="genome binned from sequencing reads available in SRX4043871; metagenomic; derived from metagenome: animal metagenome" CDS 1..339 /note="No HHBLITS hits" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AXH73269.1" /translation="MYKKNPIQQTSIERNTSYEAVTIEGKVRRMLSNKEPIKDGAQLI YTERKDGVLPSYNIRTDRWEVAVEAMSGVSKAHITKREERAKIIKMDDKKEDGKPDSI QGTDNTAEKQ" CDS 428..1213 /inference="similar to AA sequence:UniProtKB:A0A0G2UEA3_9VIRU" /note="DNA pilot protein VP2" /codon_start=1 /transl_table=11 /product="DNA pilot protein" /protein_id="AXH73270.1" /translation="MPIGELGMAGVGAAAGVVNNVMDIAFGGMRQKQQLKGQKKALEQ QNEANYDMWQKTNYGPQKEQMKAAGLNPGLMYGMGGGAGGQSMGASAMPSAGAQGGGG MDIGAAAQIALIREQARGLKIDNDKKLAETPGAGAQSDMTVQERDSRKGWTSVMSKTF GDEQEARQAIASTIGEMWKDGKLREGAEADLKEKLLKNAKTEEEKKAIMKGIEKMGVD IRTGQLNNEILELEKEMQTELGIDSKSPTWLKMIGRLFMKYFK" CDS 1168..2217 /inference="protein motif:CDD:222782" /codon_start=1 /transl_table=11 /product="putative replication initiation protein" /protein_id="AXH73274.1" /translation="MAKNDRKIIYEILQIMCLYPKLIKNPKYKMNKKNGGNIPPVNDE RVTMVPIGCQTCIECRGQKARDWNVRLQEDIKDNTNGQYWVLTYSTESIKKLTTEIQR YKDKKGKPYEGYKLDNAIAIRSMRYFLERWRKKYGKSLRHWAVTELGGKHTEHLHINI IAWTDEQEIVESIWQYGHVWRGYISKKTGKIERTYVTAKTVNYIVKYVSKVDAKHINY QAIVLTSPGIGRRYIEHGDARKNKYNEKKTEERYRAPNGAKMAIPIYWRNKIYTDEER EKLWLQKLDKNERWVCGELVAADDEKTYNNVLDYHRRRTKQIGYPDPDERWNKREYEE ARRILIQRNRIQEKE" CDS complement(1532..2233) /note="No HHBLITS hits" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AXH73272.1" /translation="MAVGYITPFPVFCFSVLVFFLLLRILFYSIAHPDPDSLFVLFVS CDNPVRCYMFFRHLLPPALHIPNVHFYPVFEAIASLFLHLYIFCFSNILVLPFLHRLV LYISPPFFSHCICFFERLHALCIFFLFLAMLVLSPGNLYALHLPYLHISLCNLLFSLL RMSVLFFLFFCLCSLSIHVRIAIYFLLFPAHLSMLLCLYAGVLYVFRLILLLPNAVGF FRIFFSIFPGSISYS" CDS complement(2695..2964) /inference="similar to AA sequence:UniProtKB:A0A0P4XNE9_9CRUS" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AXH73271.1" /translation="MWKGVPSTSVFSINCVDSCWSVSPICPFRISSFFFIVFLFFGLL LCLTILEVLSSFLLHSIECVFLPGSHSFQRCICPRASFFFLFLFS" CDS 3180..5225 /inference="similar to AA sequence:INSD:ARQ16052.1" /note="putative VP1" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AXH73273.1" /translation="MKKTLGGDRLGAGNKMTVDTHHFERSTHDTGYIWRSTMSAGTLV PFMHEVALPGDTIDIHLNCDIKTHPTIGPLFGSYKVQLDVFTVPMRLYHSALHNNRLG IGMNMAQIKLPKVRLNTNDAQVAYDPNDYTNTQIHPSCIFAYLGIRGVGTLDPADIAS NAAERSFNAIAWLAYWEIYKNYYANKQEEIGYVITKALVFNAGTTTTNVLANSTSLGT VGTPVIVNLTYNDVIHVDHTGAAPDPDSVFIQLNDGTVGNAGRLTLREITIPGSIVTE SATRIGGVYNFAKFGNKSSVYWTILTSVEQVQAPPRLEPFPLEDIDKMRDEILQSPWN VEFAIYDGSTSLLPYAYTLDNATVTGGKRYQMVMNNQEGLGVKTYQSDLFNNWLSTEW LDGTGGVNEITAIDTSSGTFSVESFVLAEKVYKMLNDIAVSGGSYNDWIGAVYDHEAF NRAESPVYMGGLIKELTFQEVVSNSAAATAGGSADNGGQPLGTLAGKGVMSGKHKGGT VVVSVSEPSYIMGIISLTPRIDYSQGNKWDVNLDTLDDLHKPHLDQIGFQELITEQMA WWTTQKNAGALNNTYTTKSAGKQPAWINYMTNVNEVHGNFAIPSNEGFMVLTRRYEPI QIAPGVVDIKDLTTYIDPVKFNHIFAETSLDAQNFWTQISVDMTSRRKMSAKLMPNL" ORIGIN 1 atgtacaaaa aaaacccgat tcaacagaca agcatagaaa ggaacacaag ctacgaggca 61 gtaacaattg aaggaaaagt aaggagaatg ctaagcaata aagaaccgat aaaagatggg 121 gcacaactaa tatacacgga aagaaaagat ggagtactac caagttacaa cataagaaca 181 gatagatggg aggtggcagt ggaagcaatg agcggagtga gcaaagcaca cataacaaag 241 agagaagaga gggctaaaat tatcaaaatg gatgataaaa aagaggacgg gaaacccgat 301 agtatacaag gaacagacaa tacggctgaa aagcagtagc agagcggcgg tacgcatgta 361 tactaatata tcaagaaggt agaggtacaa tttttaaaaa aaatcgcgaa aaacaaagga 421 ggcaaaaatg ccaataggag aactcggaat ggcgggcgtg ggagccgcag ccggagtagt 481 aaataatgtc atggacatag cattcggagg aatgagacag aaacagcagt taaaagggca 541 aaaaaaggcc ctagagcagc agaatgaagc aaactatgat atgtggcaaa aaactaatta 601 tgggccacag aaggagcaaa tgaaagccgc aggattaaat cctggactaa tgtatggcat 661 gggaggaggc gcaggaggcc aaagtatggg agcaagtgca atgccaagcg ctggagcgca 721 aggaggcgga ggcatggata taggagcagc agcgcaaata gcgctgataa gagaacaggc 781 aaggggacta aaaatagata atgataaaaa actggcagaa acaccaggtg caggagcaca 841 atcagatatg acagttcaag aaagagacag cagaaaagga tggacatcag taatgagtaa 901 aacgttcgga gacgaacaag aagcaaggca ggcaatagca agcacaatag gtgaaatgtg 961 gaaagacgga aaactaagag aaggtgctga agcagacctt aaggaaaaac tactgaagaa 1021 cgcaaaaact gaagaggaga aaaaagcaat aatgaaagga atcgaaaaaa tgggggtaga 1081 cataagaaca ggacagctaa acaacgaaat actagaactt gaaaaggaaa tgcagacaga 1141 attagggata gacagtaaat cgccaacatg gctaaaaatg ataggaagat tatttatgaa 1201 atacttcaaa taatgtgtct atacccaaaa ctaataaaga atccaaaata caagatgaac 1261 aaaaagaacg gggggaatat tccccccgtt aacgatgaaa gggtaaccat ggtacccata 1321 ggatgtcaaa cctgcatcga atgcagagga caaaaggcaa gggattggaa cgtaagacta 1381 caagaggata taaaggataa tacaaacggg caatactggg tgctgacata cagcacagaa 1441 agcataaaga aactaacgac tgaaattcag cgatataaag ataagaaagg taaaccatac 1501 gagggttata aattagataa tgcaatagcg atcaggagta tgagatactt cctggaaaga 1561 tggagaaaaa aatacggaaa aagcctacgg cattgggcag taacagaatt aggcggaaaa 1621 catacagaac acctgcatat aaacataata gcatggacag atgagcagga aatagtagaa 1681 agtatatggc aatacggaca tgtatggaga ggctacataa gcaaaaaaac aggaaaaata 1741 gaacggacat acgtaacagc gaaaacagta aattacatag tgaaatatgt aagtaaggta 1801 gatgcaaagc atataaatta ccaggcgata gtactaacat cgccaggaat aggaagaaga 1861 tacatagagc atggagacgc tcgaaaaaac aaatacaatg agaaaaaaac ggaggagaga 1921 tatagagcac caaacggtgc aaaaatggca ataccaatat attggagaaa caaaatatat 1981 acagatgaag aaagagagaa gctatggctt caaaaactgg ataaaaatga acgttgggta 2041 tgtggagagc tggtggcagc agatgacgaa aaaacatata acaacgtact ggattatcac 2101 aggagacgaa caaaacaaat aggctatccg gatccggatg agcgatggaa taaaagagaa 2161 tacgaagaag caagaagaat actaatacag agaaacagaa tacaggaaaa ggagtaatat 2221 acccaacagc catgaagggt atattacgga attaagggag gttaattcta ggattacaca 2281 agccaatccc taccaattat aacccaacag ccatgaaggg ttataatagg atcctgggat 2341 tgacttatgt aacagaagac ctcgcagggg cgcacacacc atgtcccaac agccatgaag 2401 ggactatggg tgtgagacct aaagcggtcg gccggtgagt attaaaaata caaaatacac 2461 aaaataaatt tggtaatata aaatgcaata tgtaaaattg cataaacaaa atataaaata 2521 atgagagtag aaacagtagt tcacacaaat gtaaaaggtg aacaaagaaa atacctcaga 2581 ctaacatctg agaaaggtaa aggatacctg gtaaaaataa gtgatgccca agagcgagaa 2641 atcactaaca tgatagaaga agaagaagaa agcaaaaagc agctaaaatt agaactatga 2701 aaataagaat aagaaaaaaa aagacgctcg agggcaaata catcgttgaa acgaatgact 2761 accaggtaaa aaaacgcatt caatggaatg caacagaaaa ctggaaagaa cttccagaat 2821 ggttaaacaa agcagtaaac caaaaaacaa aaatacaatg aagaaaaatg aagaaatcct 2881 aaacggacaa attggagata cactccaaca agaatcaaca cagttgattg aaaaaacaga 2941 agtggaagga acacccttcc acataataac ctaccaagat agaaactgga taggactagg 3001 aaaatacagg atcagttcaa cagaatatac tgtaaaagaa ctgaaagaaa aaatctttaa 3061 tatggactat gatttcctaa taaacgtaat atttgctgtc atcgatatga ccaggcaaat 3121 ggacaaagaa ggaatcatag taacacaaaa tccagaataa caaataaaat acaaacaaaa 3181 tgaaaaaaac gctaggcgga gaccggctag gagccggaaa caaaatgacg gttgacaccc 3241 atcatttcga aagaagtacc cacgacacgg ggtacatatg gcgctcaaca atgagcgcag 3301 gaaccctggt gccattcatg catgaagtgg cactaccagg ggatacaata gacatacatc 3361 taaattgcga catcaaaaca cacccaacta ttggccctct cttcggatca tacaaagtgc 3421 aattagatgt attcacagtg ccaatgagat tataccacag cgcactgcat aataacaggc 3481 tgggtatagg aatgaacatg gcacaaataa aattaccaaa ggtaagacta aacacaaacg 3541 atgcacaagt agcatacgac ccaaatgatt acaccaacac acaaatacat cccagctgca 3601 tattcgcata cctgggcata agaggtgttg gaacgctaga cccagcagac atagcaagca 3661 atgcagcaga aagaagcttc aacgcaatag catggctagc atattgggaa atatataaaa 3721 attactatgc taacaaacag gaagaaattg gatacgtaat aacaaaagcg ttggtattca 3781 acgcaggaac aacaactaca aacgtactag caaatagtac aagtttagga acagtaggaa 3841 caccggttat agttaaccta acctacaatg acgtaataca cgtagaccat acaggagcag 3901 caccagatcc agacagtgta ttcatacaac taaacgacgg caccgtagga aatgcaggaa 3961 gactaacatt aagagaaata accatacctg gaagtatagt aacggaaagt gcaacaagaa 4021 taggcggagt atacaacttc gcaaaattcg gaaacaaaag cagcgtatac tggacgatac 4081 taacgtcagt agaacaagta caagcacccc caaggttaga accattcccc cttgaagata 4141 tagataaaat gagggatgaa atattgcaat caccatggaa cgtggaattc gcaatatatg 4201 acggtagcac atcattacta ccgtacgcgt atacactaga taacgcaaca gtaacagggg 4261 gaaaaagata ccaaatggta atgaacaatc aggaaggact aggagtaaaa acatatcaaa 4321 gtgacctatt caacaactgg ttaagtacag aatggctgga tggaacagga ggagtaaacg 4381 aaataactgc aatagataca agctcaggaa cattcagcgt agaaagcttt gtactagcag 4441 agaaagtata taaaatgctc aacgatatag cggtgagcgg aggaagttac aatgactgga 4501 tcggagcagt atacgatcac gaagcattta acagagcaga aagtccggta tatatgggcg 4561 gactaataaa ggaattgaca ttccaagaag tagtaagcaa cagcgcagca gctacagctg 4621 gaggatcagc agataacggt ggacaaccgt taggaacact cgcaggaaaa ggggtaatga 4681 gcggaaaaca taaaggcgga acagtagtag taagcgtatc agaaccaagt tacataatgg 4741 gaataataag cctgacacca agaatagatt acagtcaggg aaataaatgg gatgtaaacc 4801 tggacacact ggatgaccta cacaaaccac acctggacca gatagggttc caggaactaa 4861 taacagaaca aatggcgtgg tggacaacac aaaaaaatgc aggagcactg aacaacacat 4921 acacaacaaa aagcgcagga aaacagccgg catggataaa ttacatgaca aatgtaaatg 4981 aggtacacgg aaacttcgca ataccaagta atgaaggatt catggtatta acaaggaggt 5041 atgaaccaat acaaatagcg ccaggagtag tggatataaa ggatctaaca acatatatag 5101 atccagtaaa attcaatcac atattcgcag aaacaagtct ggatgcacag aacttctgga 5161 cacaaataag cgtggatatg acgagcagga gaaaaatgag cgcaaaacta atgcctaacc 5221 tataaaatta ggggcacagt ggacagaaaa taagggcctg aaatggccct taaacaataa 5281 aaacaaacaa a //