LOCUS MH617660 4602 bp DNA circular ENV 29-MAR-2019 DEFINITION Microviridae sp. isolate cthg720, complete genome. ACCESSION MH617660 VERSION MH617660.2 DBLINK BioProject: PRJNA393166 BioSample: SAMN09074552 Sequence Read Archive: SRR7122375 KEYWORDS ENV. SOURCE Microviridae sp. (animal metagenome) ORGANISM Microviridae sp. Viruses; Microviridae; unclassified Microviridae. REFERENCE 1 (bases 1 to 4602) AUTHORS Tisza,M., Buck,C., Pastrana,D., Welch,N. and Peretti,A. TITLE Uncovering a Universe of Circular DNA Viruses in Animal Metagenomes JOURNAL Unpublished REFERENCE 2 (bases 1 to 4602) AUTHORS Tisza,M., Buck,C., Pastrana,D., Welch,N. and Peretti,A. TITLE Direct Submission JOURNAL Submitted (12-JUL-2018) Lab of Cellular Oncology, National Cancer Institute, 37 Convent Dr., Room 4118, Bethesda, MD 20814, USA COMMENT On Mar 29, 2019 this sequence version replaced MH617660.1. ##Genome-Assembly-Data-START## Assembly Method :: SPAdes v. 3.11.0 Genome Coverage :: 34.407866x Sequencing Technology :: Illumina ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..4602 /organism="Microviridae sp." /mol_type="genomic DNA" /isolate="cthg720" /isolation_source="minnow tissue" /db_xref="taxon:2202644" /environmental_sample /country="USA" /collection_date="01-Jun-2017" /metagenome_source="animal metagenome" /note="genome binned from sequencing reads available in SRX4043871; metagenomic" CDS 1..1620 /inference="protein motif:CDD:222784" /codon_start=1 /transl_table=11 /product="major capsid protein" /protein_id="AXH77720.1" /translation="MAKYFNQPEKMNSSARFSQAPQAEIERSKFDRSHGVKTTFNAGQ LIPVYLDEVLPGDTFTMHSTGFCRMATLLKPIMDNLYLDTHYFFVPNRLVWDNWQRFM GEQDNPTDNPGDYSVPKTPFNLANGGGTLADYFGVPVGQTSSQHQISALPFRAYTLIW NEWYRDENIQDSLVFSKGDGPDAWGATMPAYRNKRKDYFTSALPWPQKGDSIFIPLGT SAPVVGDPNQAIQIAASASNNTPRTMILGPDANTSGQGNLGYGGALVTGNLIAFLADQ DSGMIADLTQATAITINDLRTAFQIQKLLERNARGGTRYIELVLAHFGVSSPDLRAIR PVYLGGGSTPISVNPVAATANVIDETIQGDLSAYATGVQNAGFSHSFTEHGYVMGIVS VRSDLTYQNGIERHWKRTTRYDYYWPATAHLGEQSIRNWELQCVGDDLVDNEVFGYQE RHAEYRYKPSRITGKFRSSDPTSLDFWHTAQDFADQPVPLNSYFLADLPAVQRVVAVP SEPHFLLDMWFSLQCARPMPVYAVPGLVDHF" CDS 1566..2459 /inference="protein motif:CDD:164789" /codon_start=1 /transl_table=11 /product="DNA pilot protein" /protein_id="AXH77721.1" /translation="MRKADAGICRAWSGRPFLMGWGEAIGSVVETGGNIWSANKANRA GKKMAREQMQFQERMSNTAHQREVKDLVAAGLNPILSADRGGASTPSGATYSPVDPKV GSSARAGYEAAVRGRTAKAQQKLLDTQVGQVESQRDLNISTAKEAEATAAKTDAERLQ IEGVTGSKTEQAASDAEYARRRISLLLEQIKNAKSARSKMAAEQELTETITGMYPTVK GIAAAVGVAGLMGGAVKGIVSARRWWKNRKEAENLKKLFDPKKKPIDITGGKKTSDGL SLKQREEMARKDWENWHNKRK" CDS 2456..2866 /inference="protein motif:CDD:164781" /codon_start=1 /transl_table=11 /product="internal scaffolding protein" /protein_id="AXH77718.1" /translation="MKMIESPFIYIDHNGEISQELPDYSATFKRRWPYQKLRVIARNP EPSVVQHSHHDQTDINLIVARFQRDGYFPPNNTNPQYADVTPLQGDLTELHNRSAQQL QEADQFAAQWKPPLEEEHHPQKSTSTSGNQSEER" CDS 3547..4371 /inference="protein motif:CDD:222782" /codon_start=1 /transl_table=11 /product="replication initiator protein" /protein_id="AXH77719.1" /translation="MSCVYPLEGWRSAKLNDNGKRPITFKVQEAFQDMPLQVPCGKCV GCARDRSMAWTVRNYHEWTQHRQSCFLTLTYQDPAPDKINKRDLQLFFKRLRESIDVP VRYFAVGEYGETTHRPHYHALLYGADLKGKSYALDDKQYGSTFIDRTWHHGSHAIGDV NYASIAYVSGYVLKKAGNEDTFNLMSKRPPIGHGWTEKYIEELERTGHVVIDGHKLPI PRKYIELYPGELQQQLTRRKRFYTTMKPDQVEHHRKARPHREANYQSAFDRRIEKL" ORIGIN 1 atggctaagt acttcaatca acccgaaaaa atgaactcca gcgcgcgctt ctcacaagcg 61 ccgcaagcag aaatcgagcg atcaaaattc gatcgttcac acggcgtaaa aacaacattc 121 aatgccgggc aactcatacc cgtctatctg gatgaagtgc tcccgggaga caccttcacc 181 atgcacagta ccggcttctg ccggatggca acactgctta agcccatcat ggacaatctt 241 tatctggata cacattactt cttcgtcccc aatcgattag tgtgggacaa ctggcaacgc 301 ttcatggggg agcaagacaa tccaacagat aatccaggtg attactccgt gccaaaaacg 361 cctttcaatt tagcgaatgg cggcggaacg ttggcggatt acttcggtgt accagtcggt 421 caaacatcaa gccagcacca gatttcagca ctaccgttca gggcatatac gctcatttgg 481 aatgagtggt accgcgatga aaacatccag gattcgctgg tattctcgaa aggagacgga 541 cccgatgcat ggggcgcaac aatgcccgca taccgcaaca agcgtaaaga ctactttaca 601 agcgcacttc cctggccaca aaaaggggat tcaatattca ttcccttggg aacatcggca 661 cccgtagttg gcgacccaaa tcaggcgata caaatagccg cgagtgcctc taataatact 721 cccaggacaa tgatcctggg accggatgcg aatacaagcg gacaaggtaa cttagggtat 781 ggcggagcat tggtaacagg aaatttgatc gcattcttgg cggatcaaga ttcagggatg 841 atagcagacc ttactcaggc aacagcaatt acaataaatg acctccggac cgcttttcaa 901 atccagaaac ttctggagcg caatgcaagg ggaggaactc gttatataga gctggtgctc 961 gcgcatttcg gcgtaagctc ccccgacctc cgggcgataa ggcccgtgta tcttggcgga 1021 gggagcacgc ccatctctgt gaaccccgtc gcagcaacag cgaacgtgat agacgaaacg 1081 attcaaggcg atctgtctgc gtatgcgaca ggtgttcaaa atgccggttt ctcgcactca 1141 ttcactgaac acggatacgt catggggatc gtgagtgtgc gttcagatct cacataccag 1201 aacggtatcg aacgccattg gaaaagaaca acgcgttacg attactactg gccagcaaca 1261 gcacacttgg gcgaacaaag cattcgcaat tgggaactgc aatgcgtagg cgatgacttg 1321 gtagataatg aagtattcgg ataccaggag cgacacgcgg aataccggta caagccatca 1381 aggataacag gcaagttcag gtcttcggat ccaacgtcgc tggatttctg gcatacagca 1441 caagatttcg ctgatcaacc ggtaccatta aattcgtact tcttagcaga cttacccgcg 1501 gtacaacgag tcgtagcagt accatcagag ccacactttc tcctggatat gtggttctca 1561 ctgcaatgcg caaggccgat gccggtatat gccgtgcctg gtctggtaga ccatttctga 1621 tgggatgggg tgaagcaatt ggcagtgtag tagaaacagg cggcaatatt tggtccgcca 1681 acaaagcgaa tcgcgccggt aaaaagatgg cgcgagagca gatgcaattc caggagagaa 1741 tgtctaatac ggcgcatcaa cgggaggtaa aagatttagt tgcagctggg ctgaatccta 1801 ttctgtcagc ggaccgcggt ggagcgagca cgccgtcagg cgccacctac tctcccgtag 1861 acccaaaagt aggaagttcg gcaagagccg ggtatgaggc cgccgtccgg ggacggacgg 1921 ccaaggccca gcagaaattg ctggataccc aggttgggca ggtggagtca cagagagacc 1981 tgaacatctc aacagcaaaa gaagcggaag caaccgccgc aaaaacggat gcggagcggt 2041 tacaaataga gggagtgacc ggcagtaaaa cagagcaagc ggctagcgat gcggaatatg 2101 ccaggcggcg cataagccta ttattggaac aaataaaaaa cgcgaagtca gccaggagta 2161 aaatggctgc tgagcaagaa ttgacagaaa caataacagg catgtacccg acagtgaaag 2221 gcattgccgc ggcagtcggc gtagccggac tgatgggagg agccgtaaaa ggaattgtct 2281 ctgccaggcg ttggtggaaa aatcgtaaag aagctgagaa tcttaaaaag ctttttgacc 2341 ccaagaaaaa gcccatagat ataacaggcg gcaagaaaac aagtgacgga ttgagcctaa 2401 agcaacgtga ggaaatggcc cgtaaagact gggaaaattg gcataacaag aggaaatgaa 2461 aatgatcgaa tcaccattca tctatattga ccacaacggg gaaatctccc aggagttacc 2521 cgattacagc gccaccttca aaagaaggtg gccgtatcaa aaattgcgag tgatcgcaag 2581 gaatcctgaa ccgtccgtgg ttcagcattc ccatcatgat cagacggata ttaatctgat 2641 cgttgctcgt tttcagcgag acggctactt tcctccaaac aacacaaatc cgcagtatgc 2701 ggatgtaaca ccacttcagg gtgatctgac agaactgcac aaccgttcgg ctcagcaact 2761 tcaggaagca gatcaatttg cagctcagtg gaaaccgccc ctggaggagg aacatcatcc 2821 acaaaagtca acgtcaacgt ctggaaacca atccgaagag cggtagcaac tgcggaatcc 2881 aatcgcttga aagacgcata gccgcctttc aagcgtttta aataatattg agtaccatct 2941 aaaccgatcg ggttcaccag aaagaacccg tacggactat gaaacacttc gacccttgaa 3001 aagaacccat ttctaaacaa aatcttagct tctttaatag tacattcaaa cataacgtaa 3061 actccggaaa ataaaccgct aaatgcggta acagcttaat aataacacaa aatcaaataa 3121 aatcaagtaa aatagtaaaa aataacaaaa atagagtaaa tcttgtaaaa tagaaaacct 3181 tcgtcctaat caaccgggtt tacgcctccg gcgaaccgca acaatgaaag cacataggtt 3241 gcgccgcgcc ggaaaaagtg agtcagtctt accactcacg ctcaaataac caatctatat 3301 atctctaacc aaacaccata actaaaaatc attatacaaa aaccaaatag gcggatagcc 3361 tgagggcttg tgacctcctc gatccaacaa gccctactga cagaagtcag taaaacaacg 3421 aaaaggagca tttatgcgac gttcacgaac caactatcgt aagtcccgcc gccagttcac 3481 ccgcacagca aagtcttctc ctagggtaaa tcgcattact ccaatgcgtg gtggaatcag 3541 gctctgatgt cctgcgtcta tccccttgag gggtggcgct cggcaaaact gaatgacaat 3601 ggcaagcggc ctattacgtt caaggtgcag gaagcattcc aggacatgcc gctacaagtc 3661 ccttgtggta aatgcgtcgg gtgtgcgcga gatagaagca tggcctggac agtcagaaat 3721 tatcatgaat ggacccagca cagacaatcc tgctttctca cccttaccta tcaagacccg 3781 gctcccgata aaattaataa gcgtgatctt cagctctttt ttaagcgctt gcgcgaatca 3841 attgacgtgc ccgttagata tttcgccgtc ggcgaatatg gagaaaccac acatcgaccc 3901 cactatcacg ccctcctata cggggcggac ctgaaaggaa aaagctatgc tctggatgat 3961 aaacaatatg gctcgacgtt tattgatcgc acgtggcatc acggctctca tgctattggc 4021 gacgtcaatt acgcttctat tgcatatgta tccggctacg tactcaaaaa agccggtaac 4081 gaagacacct tcaatctcat gtcaaaacgt ccaccgattg gccatggatg gaccgagaaa 4141 tatattgaag agctcgaacg aacaggccac gtcgttatcg acgggcataa acttccaata 4201 ccccgtaagt acatcgaact ctaccctggg gagctacagc agcaactcac caggagaaaa 4261 cgcttttaca ccaccatgaa gcccgatcag gtggagcacc atagaaaagc acggccgcac 4321 agggaagcca actaccaatc agcattcgat agaaggatag aaaaattatg aaatgcatat 4381 ttcagttaat ggaaacaagc gagggtagac gttcacagcc aatagagcta acgcctcagg 4441 aaatggcgaa agtattgagt gaaaaattca cgccagaaca gttagaaaat caggcaatac 4501 tcgtgctcat gcaaaaattt gatggccacg aggaatacga cttcagtgtc gcgccttata 4561 tgatggcgga cacctttata aatcacttcg gagtgcaata aa //