LOCUS MH616677 4223 bp DNA circular ENV 05-AUG-2018 DEFINITION Microviridae sp. isolate cthi937, complete genome. ACCESSION MH616677 VERSION MH616677.1 DBLINK BioProject: PRJNA393166 BioSample: SAMN09074552 Sequence Read Archive: SRR7122375 KEYWORDS ENV. SOURCE Microviridae sp. (animal metagenome) ORGANISM Microviridae sp. Viruses; ssDNA viruses; Microviridae; unclassified Microviridae. REFERENCE 1 (bases 1 to 4223) AUTHORS Tisza,M., Buck,C., Pastrana,D., Welch,N. and Peretti,A. TITLE Uncovering a Universe of Circular DNA Viruses in Animal Metagenomes JOURNAL Unpublished REFERENCE 2 (bases 1 to 4223) AUTHORS Tisza,M., Buck,C., Pastrana,D., Welch,N. and Peretti,A. TITLE Direct Submission JOURNAL Submitted (12-JUL-2018) Lab of Cellular Oncology, National Cancer Institute, 37 Convent Dr., Room 4118, Bethesda, MD 20814, USA COMMENT ##Genome-Assembly-Data-START## Assembly Method :: SPAdes v. 3.11.0 Genome Coverage :: 171.246744x Sequencing Technology :: Illumina ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..4223 /organism="Microviridae sp." /mol_type="genomic DNA" /isolate="cthi937" /isolation_source="minnow tissue" /db_xref="taxon:2202644" /environmental_sample /country="USA" /collection_date="01-Jun-2017" /note="genome binned from sequencing reads available in SRX4043871; metagenomic; derived from metagenome: animal metagenome" CDS 1..1611 /inference="protein motif:CDD:222784" /codon_start=1 /transl_table=11 /product="major capsid protein" /protein_id="AXH73191.1" /translation="MPNIFNSVAVKKPKRSKFDLSHEVKMSMNMGTLTPCLVLPCVPG DRIKLNAQALVRMAPMIAPLMHRVNATIHYFFVPNRLIWDNWENFITKTTVGTPAVVP ELPVIYLSRNRYDLFPKMDYMGLPRPQSDAAEIAVSALPFAAYNKIYNEYYRQQYVQP EILDQLVDGSNMANLAALMTMKQRAWMHDYFTSGLPFAQKGEEVTVPLGDIELKPDWY ADDQKPTWDTHLGSGDTLSGFQPLNVSADGSGPSTSPSSDPFEAGGQYMQVENTPEDP YRRQAYDPNGSLQAAPVTINELRRAESLQAFLELLARGGSRYVEYIKNFFNVKPEDYR LQRPEFICGISSPVQISEVLNTTGNDGQLPQGNMSGHGAAVVRGNAGTYSCKEHGYII GIMSVTPVTAYQQGIPRDYLKYETEDFFVPQFEGLGEQNVWNAELYCTLDGVVDDTGT FAYVPRHSEYKYMPSRVAGDFKTSLSYWHLGRIFDELPTLSPEFLECNPRTDIFAVTD PDAQKLYVHLANMINASRPMRKYTIPTL" CDS 1830..2669 /inference="protein motif:CDD:222782" /codon_start=1 /transl_table=11 /product="putative replication initiation protein" /protein_id="AXH73192.1" /translation="MKCYFHKIVDGNYRACGKCFPCKMQRINDWTTRLHFEELNSHSA DFVTLTYNYENLKFPSGKARSRNYGTLYKRDVQLFIKRLRRSDKRPKSLLRSHPIKYI SCGEYGTKNKRPHYHLIIFNATQNGIDAAWRNGDKPMGQIHYDPVNPQTIAYVVGYIA NSWELRRDSRLRDFQLQLPFLTFSKGLGANYVTPAAVSFHTSEKNLLNRCYVTVDGQK RHMPKYLRQKIYSATAWKSIKEHLQVIAEIPPPIPVPDTDPANIKIFNDRILKTFRKE TIY" CDS 2672..3052 /inference="similar to AA sequence:UniProtKB:A0A0G2UEG1_9VIRU" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AXH73189.1" /translation="MAYKLFTPHPQQRERGFSGSCTVPDQSLTIKQILERHTRGQRIP DKLIGYYDDEQDPLGLDGTNWETLDLSEKHDIMRQQKEKVSHLKTQYESQSKKLIQSQ KDAENRKKEALKAELRAEMEAQKP" CDS 3128..3988 /inference="similar to AA sequence:INSD:AKI26950.1" /note="DNA pilot protein VP2" /codon_start=1 /transl_table=11 /product="DNA pilot protein" /protein_id="AXH73190.1" /translation="MPLPVISPLVTAGIEAGSSLLGGLFNKFFGNQQHAQSIDDWNMQ NAYNSPKAQMGRLKDAGLNPNLVYGQLQNGNAPQVPQQAPSKVNLEGIYSQAISLANQ NKQIEQTVENLKTNQQLMQANIELSKANTELRGSQGADLQSRISNRDILTPYQVQLSK SNIAKADADVMLKGISGKFQLDENERRQALTQSNLARSLETILNMRRDRARTDADILR IQQQTKNMVSQKELFDLDIALQKKGIQKSDPAYMRILTRAINGASFPELKAEIDSLGF MGAKLSNMYK" ORIGIN 1 atgcctaaca tctttaacag cgtagcggta aaaaaaccca aacgctcaaa atttgacctt 61 tctcatgagg taaaaatgtc catgaatatg ggcactctaa ccccttgtct tgttctccct 121 tgtgttccgg gtgatcgtat taaactcaat gctcaggcac tcgttcgtat ggctccaatg 181 attgcccctt taatgcaccg agtgaatgct actattcatt atttctttgt tcctaatcgc 241 cttatctggg ataattggga aaactttata acaaaaacta cggtaggtac acctgctgtt 301 gttcctgaac ttccggttat ttatctgtct cgtaaccgtt atgatctatt tcctaaaatg 361 gattatatgg gtcttcctcg tcctcagtct gatgctgcgg aaattgctgt ttctgccctt 421 ccctttgctg cctataacaa aatttacaat gaatactatc ggcagcaata cgtccagcct 481 gagatcctcg atcaattagt tgacggttct aatatggcta atcttgctgc tcttatgacc 541 atgaagcaac gggcatggat gcatgactat tttacctctg gtcttccatt cgctcagaaa 601 ggcgaagaag ttactgttcc acttggcgat attgaactaa aaccagattg gtacgcagac 661 gaccaaaaac ctacttggga cactcacttg ggttctggtg atactttaag tggtttccaa 721 cctcttaatg tttctgctga cggttcaggt ccttccactt ctccttcttc tgatcctttt 781 gaagctggtg gacaatatat gcaagttgaa aacactccag aagatcctta ccgtcgccaa 841 gcttatgacc ctaatggatc acttcaagct gctcctgtta cgatcaatga attacgtcgt 901 gctgaaagcc ttcaagcttt cttagaatta cttgctcgtg gtggttctcg ttatgttgaa 961 tacatcaaaa acttcttcaa tgttaaacct gaagattatc gcttacagcg tcctgaattt 1021 atttgtggta ttagttctcc tgtacaaatt tctgaagtac ttaatactac cggcaacgac 1081 ggtcaactgc cgcaaggtaa tatgtctggg catggtgctg ctgttgtacg tggcaacgct 1141 ggtacttata gctgtaaaga gcatggttac atcatcggaa ttatgtccgt aacccctgta 1201 actgcttatc agcaaggtat tccacgtgac tatcttaaat atgaaactga agatttcttt 1261 gtacctcaat tcgaaggact tggcgaacaa aatgtttgga atgctgaact atattgcact 1321 cttgacggtg tagttgatga cactggtacc tttgcctatg tccccagaca ttccgaatat 1381 aaatacatgc cttcgcgtgt agctggagac tttaaaacct ccttatctta ttggcatctt 1441 ggcagaatat tcgacgaact gcctaccctg tcacctgaat ttctagaatg taatcctcgt 1501 actgacatct ttgcggttac tgatcctgac gcccaaaaac tatatgtaca cctggctaac 1561 atgataaatg catctcgtcc aatgcgtaag tacacaatac ctacgctata aaaaaacgcg 1621 ttaaaacccc cttcggcttg ccgttggggg tattaatgca aaccaaccgt agggcggtag 1681 ctttcgcacc tgttaacctg tcgctctcgc acgtaaaatg aggaacgaag tgacgaataa 1741 cacaagcgga gcgacgtggg aaaaagggcg taaagctttg ctaaaaaaat tttttttgtt 1801 ttacaaaaaa atacttttaa tttaacgcca tgaaatgcta ctttcataaa atcgttgacg 1861 gaaactaccg cgcttgcggt aagtgtttcc cttgtaaaat gcaacgaata aacgattgga 1921 ctactaggct acatttcgaa gaattaaatt ctcactctgc tgactttgta actttaactt 1981 ataactatga gaacctcaaa ttcccttctg gtaaagcacg ttcaaggaat tatggaacct 2041 tatataagcg agacgtacaa ctctttatta aacgacttcg aagatcagat aaacgcccca 2101 agtccttgtt acgatctcat ccaatcaagt atatatcatg tggggaatat ggaactaaaa 2161 ataaacgccc acattaccat ctcattattt tcaatgccac ccaaaacgga attgatgcag 2221 cttggagaaa tggcgataaa cctatgggtc agatacatta tgacccagtt aatccccaaa 2281 ctatcgcata cgtcgtcgga tatatcgcta actcatggga gttacgcaga gactcacgtc 2341 ttcgtgactt tcaattacag cttcctttcc ttacattctc caaaggtctg ggagcaaatt 2401 acgttactcc cgctgctgta tccttccaca catcagaaaa aaaccttctc aaccgttgtt 2461 acgtcacagt tgacggacaa aaacgacaca tgccaaaata cttaaggcag aaaatatatt 2521 cagctactgc ttggaagtct ataaaagaac accttcaagt tattgcggag attcctccgc 2581 ctattcctgt acctgatact gacccagcta atatcaaaat ttttaatgat cgaattctta 2641 aaaccttccg aaaggaaaca atctactaat tatggcatac aaactcttta cacctcaccc 2701 acagcaacgc gaacgcggtt tctccggttc ttgcaccgtt cctgatcagt ctttaactat 2761 caaacagatc cttgaacgtc atactcgcgg tcagcgtatt cctgacaagc tcatcggcta 2821 ttatgacgat gaacaagacc ctttaggtct cgatggtaca aactgggaaa ctcttgacct 2881 atccgagaaa catgatatca tgagacaaca gaaagaaaag gttagtcatt taaagactca 2941 atatgagtct cagtctaaaa agcttataca atctcaaaag gacgctgaaa accggaaaaa 3001 agaggcttta aaagccgaat tacgggctga aatggaagcc caaaaaccat agtagttact 3061 tgataatact atggacagtt gacaccgtca actaaaaaag gtactttata acataaaatt 3121 ttaatatatg ccactccctg ttatttctcc tcttgtgacc gctggtattg aagccggttc 3181 ttctcttctc ggtggtctat ttaacaaatt ctttggtaac caacaacacg cccaatctat 3241 tgatgattgg aacatgcaaa atgcttataa ctctcctaag gcgcaaatgg gtcgcctaaa 3301 agacgctggt cttaacccta acttagttta tggacaatta caaaatggca atgcaccaca 3361 agtaccacaa caagctcctt ctaaagttaa tctcgaagga atttattctc aagcaatctc 3421 gctcgctaat cagaacaaac agattgagca aacagtcgaa aaccttaaga ctaatcaaca 3481 actcatgcaa gccaatattg agctctccaa agcaaatacg gaacttagag gctctcaagg 3541 tgctgacctc caatccagaa tttctaaccg cgatattctc actccctacc aggtgcaatt 3601 atctaaatct aacatcgcaa aagctgacgc ggacgtaatg ctcaaaggta tttccggtaa 3661 atttcagtta gatgaaaatg aacgacgtca agctctaacc caatctaatc ttgcccgttc 3721 tcttgaaact attctaaata tgcgtcgtga tcgcgctcgt actgacgctg atattcttcg 3781 tattcaacaa caaactaaaa atatggtatc tcaaaaagaa cttttcgatc tcgatatcgc 3841 attacaaaaa aagggtatac aaaaatctga ccctgcctat atgcgtattc ttactcgcgc 3901 tattaatggc gcttcatttc ctgaattaaa ggccgaaatt gattcactag gctttatggg 3961 agctaaacta agcaatatgt ataaatagct gctctgtcgt ggtgacaaca gcacccctta 4021 gctagtcccc ccgttaagtc ggggggcttt tatcccccta gctaggggaa aaaaaaccat 4081 taaaccttaa acccctatat atgaaacgtt tcaaaaaaaa atcctttgga aaaggtcgca 4141 aacgtctaag gaaaagccgt tctaagcgct tcaacactta tttaatttca cgaggcggta 4201 ctcgtctcta aaacctctcc tct //