LOCUS MN582053 4250 bp DNA circular ENV 18-NOV-2019 DEFINITION Microviridae sp. ctjWc39, complete genome. ACCESSION MN582053 VERSION MN582053.1 DBLINK BioProject: PRJNA540674 BioSample: SAMN11552893, SAMN11552892, SAMN11552894 Sequence Read Archive: SRR8996939, SRR8996941, SRR8996944 KEYWORDS ENV. SOURCE Microviridae sp. ctjWc39 (freshwater metagenome) ORGANISM Microviridae sp. ctjWc39 Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae. REFERENCE 1 (bases 1 to 4250) AUTHORS Malki,K. and Breitbart,M. TITLE Florida's Freshwater Springs JOURNAL Unpublished REFERENCE 2 (bases 1 to 4250) AUTHORS Malki,K. and Breitbart,M. TITLE Direct Submission JOURNAL Submitted (16-OCT-2019) College of Marine Science, University of South Florida, 830 1st St S, Saint Petersburg, FL 33701, USA COMMENT Submissions were annotated with Cenote-Taker2 available at https://github.com/mtisza1/Cenote-Taker2. ##Genome-Assembly-Data-START## Assembly Method :: SPAdes v. 3.12 Genome Coverage :: 16.900941x Sequencing Technology :: Illumina ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..4250 /organism="Microviridae sp. ctjWc39" /mol_type="genomic DNA" /isolate="ctjWc39" /isolation_source="Volusia Spring" /db_xref="taxon:2656702" /environmental_sample /country="USA" /collection_date="22-May-2017" /metagenome_source="freshwater metagenome" /note="genome binned from sequencing reads available in SRX5775735,SRX5775734,SRX5775731; metagenomic" CDS 1..360 /note="No HHsearch hits" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QGH72129.1" /translation="MTLTHTTNLHLTPNVRKSNIKTQSTYQYLMSPGTFTSSIYNVPG KVYWEFPVDKGGWLGASCTGALLVANPIDTNSSKFFGSKQKSKKRAGSLPNLFLTIDG KASKLALASLTWSMADL" CDS complement(138..557) /inference="protein motif:PFAM:PF09675.9" /codon_start=1 /transl_table=11 /product="scaffold protein" /protein_id="QGH72126.1" /translation="MRDSSGNYVRVRKEFSKPSMTKQSFREECDINLIMKKFKKVMGA DFLSRYSGYAGGQFGDFSQVGDYRSAIDQVNEARASFEALPSIVRKRFGNDPALFLDF CLDPKNLDELVSMGLATKSAPVQDAPSQPPLSTGNSQ" CDS complement(564..1094) /inference="similar to AA sequence:UniProtKB:A0A0C5AFV1_9VIRU" /codon_start=1 /transl_table=11 /product="putative minor capsid protein" /protein_id="QGH72125.1" /translation="MVLDTLGAFGTGALNYLGAREANKTNKKIAREQMAFQERMSNTA YQRSVQDLKAAGLNPILAYSQGGASSPGGASTSVQNEFSGASSSALDAKRMAAEVENM KVQNENLKAMNEQIRSQASLNNANTALAYKNADIIDVKLPTLKQDADIDTSTFGWIMK HAERLNPFRGIFKASK" CDS complement(1103..2773) /inference="protein motif:PFAM:PF02305.16" /codon_start=1 /transl_table=11 /product="major capsid protein" /protein_id="QGH72124.1" /translation="MNPPAKPWEENLKRGLIMLRGSVPSVDSKALHTFSRAPQAAIPR SKFNRSHGVKTTFNGGLLVPVLVDEMLPGDTFNLKMHAFARMATLHFPIMDNLYLESF FFAVPYRLVWDNWEKFNGYQKNPGDPTDFLIPQMTAPAMGGAEESGLADYMGIPTKIN SLSYNSLHFRAYNLIWNDWFRDENLQNSAPVDTDDGPDDYTDYRFCLRRGKRYDYFTS CLPFPQKGPDVTLPLGSTAPLVGNGGSTYIHTDFDTTNREMVVAGTTNPLSASVLYSN SYAGTGSFGSSLKFSTVPGDIGLDVDLTDATAATINQLRQAFQTQKLYERDARGGTRY TEIIKSHFGVTSPDMRLQRPEYLGGGSTPIMVNPVAQTSNTADSTELGRLAAYGVCAP HGHGFVYSATEHCLILGLVCVRADINYQTGLERMWSRQTRLDFYWPALSHIGEQAVLN KEIYAQGTSADDDVFGYQERYAEYRYKPSQITGLFRSNASGSLDSWHLAQDFASLPVL NDEFIVVNDPINRVVDVTPPDFLFDCFFSYHCVRPMPVYSVPGLVDHF" CDS complement(2853..3119) /inference="similar to AA sequence:UniProtKB:A0A0G2UEC9_9VIRU" /note="DNA binding protein VP5" /codon_start=1 /transl_table=11 /product="DNA binding protein" /protein_id="QGH72128.1" /translation="MLLKVFTIYDSKAEAYLQPFFLKNKGEAIRALTDIVAKPDHNFC KYPEDFTLFELGVFDDSTSRFELYDTPHSVCVLIELKRSVTPSA" CDS complement(3122..4069) /inference="protein motif:PFAM:PF05840.12" /codon_start=1 /transl_table=11 /product="replication associated protein" /protein_id="QGH72127.1" /translation="MPCYHPLQAYYSVRSDGKKDLHFSSELTKFASRSFHEGKKLPAS LAGSFLSLPCGRCMGCRLERSRQWAVRIMHEAQLYEDNCFITLTFDEDNLKKMCPNGS LDRKHMQNFLKRLRQKFGDRKIRTFYCGEYGDDLGRPHYHACLFNLDFTDKLFWKSVN GCRYYTSKSLRDVWTFGHSVVGDLTFDSAAYVARYCTKKVTGDKALIEDHYKGRLPEF CQASLKPGIGSGWLDRFGASDVFPLDEVVVRGVRCKPPRYYDKRLERDDPIAFEKLKL LRLERAKDKEDDNTHARLLVKEKCQQARFKKLIRTLERS" CDS complement(3969..4139) /note="No HHsearch hits" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QGH72130.1" /translation="MVKGIPARIPLIFINWVVKVLIVYAMLSSFASLLFRAFRRKEGF AFFIRAYEICFA" ORIGIN 1 atgacgctta ctcatacaac aaaccttcat ttaacaccaa atgttagaaa atccaacata 61 aagacacaat ctacttacca gtatcttatg tcacctggca catttacatc aagtatatat 121 aatgtgccag gaaaagtcta ctgggaattt ccagtagaca aaggaggttg gctaggcgca 181 tcttgtacgg gcgcactctt agtagccaac cccatagaca ccaactcatc caaattcttt 241 ggatctaaac aaaaatccaa gaaaagagca ggatcattac caaacctctt tcttacaatc 301 gacggcaaag cctcaaaact cgctctagct tcgttaactt ggtcaatggc agacctatag 361 tctcccactt ggctaaagtc gccaaattga ccgccagcgt atccgctata acgactcaaa 421 aaatcagcac ccatgacttt tttaaacttc ttcataatca aattaatatc acactcctca 481 cgaaaactct gcttagtcat agaaggctta gaaaactcct tcctcacacg aacataatta 541 ccactagaat ctctcataaa acctcactta cttgctttaa aaataccacg aaacggattc 601 aaacgctccg catgtttcat aatccaacca aacgtactag tatcaatatc agcatcctgc 661 tttaacgtag gtaacttaac atcaataata tcagcattct tataagctaa cgcagtgtta 721 gcattattca aagaagcctg cgaacgaatc tgctcattca tagccttcaa attctcattc 781 tgaaccttca tattctcaac ctcagcggcc atccgcttag catcaagagc agaagaagac 841 gcaccagaaa actcattctg aacactagta gaggcacccc ccggagaaga ggcaccccct 901 tgactataag ccaaaatagg attcaaaccg gcagctttca gatcttgcac actgcgctga 961 tacgcagtgt tactcatacg ctcctgaaaa gccatctgct ctctagcaat cttcttatta 1021 gtcttattag cctctctagc acccaaataa ttaagagcac cggtgccaaa agcacctaac 1081 gtatctaaaa ccatatatcc tcctaaaaat ggtcaacaag accaggaaca ctataaacag 1141 gcatcggacg cacacaatga taactaaaaa aacaatcaaa caaaaagtca ggaggcgtca 1201 catcaacaac tctattaata ggatcattaa caacaatgaa ctcatcatta agaacaggca 1261 aactggcaaa atcttgagcc aaatgccaag aatcaagaga accggaagca ttagacctaa 1321 acaatccagt aatctgggaa ggcttataac gatactccgc ataacgctct tgataaccaa 1381 aaacatcatc atcagcagac gtgccttgcg cataaatctc cttattaaga acagcttgct 1441 cacctatatg agaaagcgca ggccaataaa aatccaaacg agtctgacgc gaccacatac 1501 gctccaaacc agtctgataa ttaatatcag cacgaacaca cacaagaccc agaatcaaac 1561 aatgctcagt agcactataa acaaacccat gaccatgagg agcacacaca ccataagcag 1621 ccaatcggcc taactcagta gaatcagcag tattagaggt ctgggcaaca ggattaacca 1681 taataggggt agaaccacca ccaaggtact caggacgttg caaacgcata tcaggagatg 1741 taactccaaa atgagattta ataatctcag tataacgagt accacctcgc gcatcacgct 1801 catacaactt ctgggtctga aaagcttgac gcaactgatt aatagttgca gcagtagcat 1861 cagtcaaatc aacatctaaa ccaatatcac caggaaccgt tgaaaacttc aacgaagaac 1921 caaaactacc agtaccagca tagctattag aatacaaaac actagcactc aacggattag 1981 tagtaccagc aacaaccatc tcacgattag tagtatcaaa atccgtatga atataagtag 2041 aaccaccgtt accaactaac ggagcagtag aacctaacgg caaagtaaca tcagggcctt 2101 tctgaggaaa aggcaaacaa gacgtgaaat aatcatatct tttaccacgt ctcaaacaaa 2161 atctatagtc cgtataatca tcaggaccat catcagtatc aacaggggcg gaattctgta 2221 aattctcgtc cctaaaccaa tcattccaaa ttaaattata agcacgaaaa tgcaaagagt 2281 tataactcaa cgaattaatc ttagtaggaa tacccatata atcagctaaa ccactttcct 2341 ccgcaccgcc catagcagga gcagtcatct gaggaataag aaaatcagta ggatcaccag 2401 gattcttctg ataaccatta aacttttccc aattgtccca aaccaatcta taagggacag 2461 caaaaaaaaa agactccaaa tacaaattat ccattatcgg aaagtgaaga gttgccatac 2521 gagcaaaagc atgcatcttc aaattaaacg tatcacctgg caacatctca tcaacaagaa 2581 caggaactaa caaacctcca ttaaacgtag tcttaacacc gtgagaacga ttaaacttac 2641 tacgaggaat agcagcttga ggagcacgac taaacgtatg taaagcctta ctatcaacac 2701 taggtacgga acctcttaac ataatcaaac ctctttttaa attttcctcc cagggcttcg 2761 ccgggggatt cataaaacca atttaatcca atacaacact aaacaaaacc aagggtggcc 2821 taaactgggg ggtaaacccc ccagcccccc catcacgcag aaggagtgac actgcgctta 2881 agctctatca aaacgcaaac agagtgggga gtatcataca actcaaaccg actagtagaa 2941 tcatcaaaaa cacccaactc aaacaaagta aaatcttcag gatacttaca aaaattatga 3001 tcaggcttag caacaatatc ggttaaggct ctaatagcct ctcctttatt cttcaagaaa 3061 aacggttgca aataagcctc agccttagaa tcataaatag taaaaacttt caataacata 3121 actaactcct ttctaatgta cgaatcaatt tcttaaaacg tgcctgttgg cacttctctt 3181 taactaacaa acgagcatga gtattatcat cctctttgtc cttagcacgc tccaaacgca 3241 ataacttaag cttctcaaaa gcaataggat catcacgctc taaacgctta tcataatatc 3301 gaggaggctt acaccgcaca cctcgcacta ccacctcatc gaggggaaac acatcacttg 3361 caccaaatct atctaaccaa cctgaaccta taccaggctt caaactagct tgacaaaact 3421 caggcaaacg acccttataa tggtcttcaa tcaaagcctt atctccagtc accttcttag 3481 tacagtagcg agccacataa gcagcagagt caaaagtaag atcaccgacc acgctgtgac 3541 caaatgtcca cacatctcta agagacttag acgtataata tcgacatcca ttaacactct 3601 tccaaaacaa cttatcagta aaatcaagat taaacaaaca cgcatgataa tgcggtctac 3661 ctaaatcatc accatactca ccacaataaa aagtacgaat ctttcgatca ccaaacttct 3721 gacgcaaacg ctttaaaaaa ttctgcatat gtttgcgatc aagagaacca ttaggacaca 3781 tcttcttaag attgtcctca tcaaacgtca acgttataaa acaattgtcc tcataaagct 3841 gagcctcatg cataatacgt accgcccatt ggcgagaacg ttctaaacga caacccatac 3901 aacgaccaca aggtagagag agaaacgaac cagcaagcga agcgggcaac ttcttgccct 3961 catgaaaact acgcgaagca aatttcgtaa gctctgatga aaaatgcaaa tccttctttc 4021 cgtctgaacg cacggaataa taagcttgca aaggatgata acatggcata tactatcaga 4081 accttaacta cccagttaat aaaaatgagg ggaatccttg cagggattcc cttcaccatt 4141 taggatcaaa tcctaaaacc gcccctcatc ggatgacggt gaatattcag gggatgaacc 4201 cactgagcgg ttctggaaaa taatttctta ctaccagatc tagacatctt //